Oocyte collector

Abstract

The invention discloses an oocyte collector, and belongs to the technical field of medical instruments, the oocyte collector comprises a collecting tube and a rubber plug inserted in the collecting tube, the surface of the rubber plug is provided with two mounting holes, a negative pressure tube and a suction tube are respectively fixed in the two mounting holes, a handle is fixed outside the suction tube, and the negative pressure tube is connected with the suction tube. An egg taking needle is inserted into one end of the handle, an ultrasonic developing coating is arranged at the end, away from the handle, of the egg taking needle, a fixing ring is fixed to the outer wall of one side of the handle, a plurality of fixing holes are formed in the positions, corresponding to the fixing ring, of the handle, and optical fiber light transmitting bundles are installed in the fixing holes. According to the oocyte collector, the puncture accuracy and the survival rate of oocytes are remarkably improved, the operation difficulty and the complication risk can be reduced, and the clinical application prospect is wide.

Description

Technical Field

[0001] The invention belongs to the technical field of medical appliances, and in particular relates to an oocyte collector. Background Art

[0002] Successful oocyte collection is a critical step in both modern assisted reproductive medicine and livestock breeding technology. In the medical field, oocyte collection is the cornerstone of assisted reproductive technologies such as in vitro fertilization and embryo culture. The performance, ease of use, and safety of the collection device directly impact the success rate of the assisted reproductive process and the patient experience. In livestock farming, the efficient collection of oocytes from the ovaries of cattle and sheep carcasses is crucial for the rapid breeding of superior breeds and improving the economic benefits of farming. It is a prerequisite for the large-scale application of livestock breeding technologies such as in vitro fertilization and embryo transfer.

[0003] However, existing oocyte collectors still have certain deficiencies in clinical and animal husbandry practice: Low puncture positioning accuracy and high risk of tissue damage: While existing egg retrieval needles utilize ultrasound guidance (in the medical field) or rely on experience (in the livestock field), they lack specific imaging structures and rely solely on the weak ultrasonic reflection of the needle itself (in the medical field) or visual observation (in the livestock field), resulting in blurred boundaries and unclear depth markings during operation. In medical settings, doctors rely on experience to determine the angle and depth of needle insertion. This can lead to the egg retrieval needle mistakenly piercing the deep layers of the ovarian cortex or the surrounding vascular plexus due to visual deviations, causing complications such as bleeding and infection. When collecting ovaries from cattle and sheep carcasses, due to changes in the carcass ovary's structural state after removal from the body and the lack of reference such as live blood flow, puncture deviations are more likely to cause excessive damage to ovarian tissue and affect oocyte activity. This is especially true for carcass ovaries with dense follicles, where the risk of damage is significantly increased. Furthermore, the needle lacks clear axial scale markings, making it difficult to maintain a consistent depth during repeated punctures, further increasing the probability of tissue damage.

[0004] Lack of real-time vision limits collection efficiency: During traditional collection procedures, doctors in the medical field can only indirectly observe the location of follicles through in vitro ultrasound, while in the livestock sector, these procedures often rely on autopsy or blind puncture, neither of which can obtain real-time morphological information about the interior of the follicle (such as follicular wall thickness and the distribution of oocytes within the follicle). This results in the egg retrieval needle often mistakenly puncturing the extrafollicular space due to "blind puncture," or puncturing the follicle but not aiming at the area where oocytes are concentrated, resulting in "empty suction" and significantly reducing the oocyte recovery rate. In the collection of ovaries from cattle and sheep carcasses, the success rate is even lower due to the unstable state of the follicles after the ovaries are removed from the body. Multiple repeated punctures are required, which not only prolongs the operation time but also affects the quality of the oocytes due to excessive handling. Summary of the Invention

[0005] The object of the present invention is to provide an oocyte collector to solve the problems of low puncture positioning accuracy, high risk of tissue damage, lack of real-time field of view, and limited collection efficiency proposed in the above background technology.

[0006] To achieve the above object, the application provides the following technical scheme: an oocyte collector, comprising a collecting tube and a rubber plug inserted into the collecting tube, a surface of the rubber plug is provided with two mounting holes, and a negative pressure tube and a suction tube are respectively fixed in the two mounting holes, an outer part of the suction tube is fixed with a handle, one end of the handle is inserted with an oocyte extraction needle, an ultrasonic imaging plating layer is arranged at a distal end of the oocyte extraction needle away from the handle, a fixed ring is fixed to one side of the outer wall of the handle, a plurality of fixed holes are arranged at corresponding positions of the handle and the fixed ring, and an optical fiber light guide beam is mounted in the fixed holes.

[0007] In a further embodiment, an air suction head is mounted at one end of the negative pressure tube inside the collecting tube, and a baffle is fixed in the air suction head.

[0008] In a further embodiment, a discharging head is fixed at one end of the suction tube inside the collecting tube, and a discharging end of the discharging head is close to an inner wall of the collecting tube.

[0009] In a further embodiment, a flushing tube is mounted at one end of the handle, a reversing valve is mounted at a connection part of the suction tube and the flushing tube, the suction tube and the flushing tube form a suction path and a flushing path respectively by cooperating with the reversing valve, and a rotary control switch for controlling the reversing valve is mounted on one side of the outer wall of the handle.

[0010] In a further embodiment, a moving hole is arranged on the outer wall of the handle, and a pressing block is movably connected in the moving hole, a fixed ring is fixed to the outer wall of the pressing block, and an elastic pad is sleeved on the outer part of the pressing block.

[0011] In a further embodiment, an adjusting piston is fixed to an end face of the pressing block inside the handle, and a limiting ring movably connected with the handle is fixed to the outer part of the adjusting piston.

[0012] In a further embodiment, the ultrasonic imaging plating layer is tungsten alloy, and is distributed at equal intervals along an axial direction of the oocyte extraction needle.

[0013] In a further embodiment, a pointing ridge is arranged on the surface of the handle, and is parallel to an axis of the oocyte extraction needle.

[0014] The application has the following technical effects and advantages: The oocyte collector has the tungsten alloy ultrasonic imaging plating layer at the distal end of the oocyte extraction needle distributed at equal intervals along the axial direction, forms clear depth marks under the ultrasonic image, and cooperates with the optical fiber light guide beam mounted between the handle and the fixed ring to transmit the image inside the follicle in real time, realizes the double guidance of "ultrasonic positioning + real-time visual field", significantly reduces the puncture deviation, and reduces the damage to the surrounding tissues. The reversing valve and rotary control switch integrated in the handle can quickly switch between the suction and flushing pathways. This operation can be completed by a single person without plugging or unplugging tubing, significantly shortening the surgical time. The pointing ridge on the handle surface is parallel to the axis of the egg retrieval needle, allowing for quick confirmation of the needle tip direction and improving operational stability. The pressing block cooperates with the regulating piston, and the negative pressure can be fine-tuned manually by pressing to avoid cell damage caused by sudden changes in negative pressure. The discharge head at the end of the suction tube is close to the inner wall of the collection tube, reducing the impact when the oocyte enters the collection tube. The baffle inside the suction head can prevent the oocyte from being directly sucked into the negative pressure tube, further reducing the risk of damage and improving cell survival rate. The suction tube and the flushing tube are designed with independent passages through the reversing valve to avoid cross contamination. The overall structure is reliably sealed. The plug-in fit between the rubber plug and the collection tube and the fixed design of the pipeline interface reduce the leakage of egg collection fluid and the incidence of complications such as postoperative infection. The oocyte collector significantly improves the puncture accuracy and oocyte survival rate, reduces the difficulty of operation and the risk of complications, and has broad clinical application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0016] Figure 1 It is a structural schematic diagram of the present invention; Figure 2 Schematic diagram of the structure of the negative pressure tube of the present invention; Figure 3 It is a schematic structural diagram of the suction tube and handle of the present invention; Figure 4 For the present invention Figure 1 Enlarged view of point A in the middle; Figure 5 For the present invention Figure 1 Enlarged view of point B in the middle; Figure 6 It is a structural schematic diagram of the pressing block and the regulating piston of the present invention.

[0017] In the figure: 1. Collection tube; 2. Rubber stopper; 3. Negative pressure tube; 4. Suction head; 5. Baffle; 6. Suction tube; 7. Handle; 8. Egg retrieval needle; 9. Ultrasonic development coating; 10. Discharge head; 11. Pointing ridge; 12. Fixing ring; 13. Fiber optic light guide; 14. Rotary control switch; 15. Press block; 16. Fixing ring; 17. Elastic pad; 18. Adjusting piston; 19. Limiting ring; 20. Flushing tube. DETAILED DESCRIPTION

[0018] In the following description, numerous specific details are provided to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention may be practiced without one or more of these details. In other instances, certain technical features well known in the art are not described to avoid confusion with the present invention.

[0019] Unless otherwise defined, the directions of up, down, left, right, front, back, inside and outside involved in this document are based on the directions of up, down, left, right, front, back, inside and outside shown in the figures of the present invention, and are explained here together.

[0020] The present invention provides Figure 1-6 The oocyte collector shown in the figure includes a collection tube 1 and a rubber plug 2 inserted into the collection tube 1. The collection tube 1 is made of medical-grade high-density polyethylene (HDPE), which has good chemical resistance and light transmittance, making it easy to observe the state of the oocytes and follicular fluid inside. The rubber plug 2 is made of medical silicone rubber, which has excellent elasticity and sealing properties and can withstand repeated insertion and removal without generating gaps. Its diameter matches the inner diameter of the collection tube 1 (interference amount 0.1-0.2mm) to ensure complete sealing after insertion. The surface of the rubber plug 2 is provided with two mounting holes, and the two mounting holes are respectively fixed There are a negative pressure tube 3 and a suction tube 6. One end of the negative pressure tube 3 located inside the collection tube 1 is equipped with a suction head 4. The other end of the negative pressure tube 3 is connected to an external negative pressure source (such as a negative pressure pump). A baffle 5 is fixed inside the suction head 4. The suction head 4 is injection-molded with medical ABS plastic and is funnel-shaped. The baffle 5 allows gas to pass through but blocks oocytes from entering the negative pressure tube 3 to prevent cell loss. The suction tube 6 is made of medical silicone with good flexibility and biocompatibility. The inner wall is polished (roughness Ra ≤ 0.1 μm) to reduce friction damage during oocyte transmission. A handle 7 is fixed to the outside of the suction tube 6. The surface of the handle 7 is frosted for easy gripping. An arc-shaped groove is provided in the middle (fitting the curvature of the finger) to reduce grip fatigue. An egg retrieval needle 8 is plugged into one end of the handle 7. A pointing ridge 11 is provided on the surface of the handle 7, which is parallel to the axis of the egg retrieval needle 8. The direction of the needle tip can be quickly confirmed by touch. The egg retrieval needle 8 is made of medical stainless steel (316L) with good strength and corrosion resistance. The needle tip is beveled at 30° (reducing puncture resistance). The tail is a tapered plug, which has an interference fit with the channel of the handle 7 and is sealed by a medical-grade silicone sealing ring. The end of the egg retrieval needle 8 away from the handle 7 is provided with an ultrasonic development coating 9. The physical vapor deposition (PVD) process is used to plate a tungsten alloy layer within the distal range of the egg retrieval needle 8. The coating thickness is 5-10μm and is evenly spaced along the axial direction (spacing 2mm), forming a scale of alternating light and dark. It is clearly displayed under B-ultrasound imaging and is convenient for accurately controlling the puncture depth. The end of the suction tube 6 located inside the collection tube 1 is fixed with a discharge head 10. The discharge end of the discharge head 10 is close to the inner wall of the collection tube 1, which can guide the oocytes to slowly flow into the collection tube 1 along the tube wall to avoid cell damage caused by direct impact; A fixing ring 12 is fixed to the outer wall of one side of the handle 7. A plurality of fixing holes are opened at the corresponding positions of the handle 7 and the fixing ring 12. An optical fiber light guide 13 is installed in the fixing hole. The optical fiber light guide 13 adopts a multi-mode quartz optical fiber. One end is fixed to the side of the handle 7 through the fixing ring 12, and the other end is connected to an external cold light source (wavelength 400-700nm, illumination 5000lux). The end of the optical fiber extends to 1mm behind the bevel of the tip of the egg retrieval needle 8 (not shown in the figure), which can transmit the internal image of the follicle to the external display screen in real time; A flushing tube 20 is installed at one end of the handle 7. A reversing valve is installed at the connection between the suction tube 6 and the flushing tube 20. The reversing valve is a micro ball valve structure (made of medical ceramic). The suction tube 6 and the flushing tube 20 cooperate with the reversing valve to form a suction path and a flushing path respectively. A rotary control switch 14 for controlling the reversing valve is installed on the outer wall of one side of the handle 7. By rotating the control switch 14, the valve core is driven to rotate to realize the switching between the suction path (connecting the egg retrieval needle 8 and the suction tube 6) and the flushing path (connecting the egg retrieval needle 8 and the flushing tube 20). The switching response time is ≤ 1 second, ensuring convenient operation. A movable hole is provided on the outer wall of the handle 7, and a pressing block 15 is movably connected in the movable hole. A fixing ring 16 is fixed to the outer wall of the pressing block 15. The fixing ring 16 can limit the moving distance of the pressing block 15, and an elastic pad 17 is sleeved on the outside of the pressing block 15. The elastic pad 17 can support the fixing ring 16 and facilitate the movement of the pressing block 15. An adjusting piston 18 is fixed to the end surface of the pressing block 15 located inside the handle 7, and a limiting ring 19 movably connected to the handle 7 is fixed to the outside of the adjusting piston 18. The limiting ring 19 can prevent the adjusting piston 18 from slipping out of the handle 7, and the negative pressure in the suction path is adjusted by the axial movement of the pressing block 15; To meet different clinical needs, the egg retrieval needle 8 is available in two sizes: 17G (suitable for follicles with a diameter of ≥15mm) and 19G (suitable for follicles with a diameter of 10-15mm). The inner diameter of the suction tube 6 can be adjusted to match (17G corresponds to an inner diameter of 2.5mm, 19G corresponds to an inner diameter of 2mm). The optical fiber light guide 13 is compatible with mainstream cold light source equipment (such as Stryker cold light source) and is connected through a standard SMA905 interface; the negative pressure tube 3 can be adapted to most brands of negative pressure pumps (such as Kangji negative pressure aspirator), and the interface is a Luer lock type.

[0021] The standard parts used in the present invention can all be purchased from the market, and special-shaped parts can be customized according to the description in the specification and drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the existing technology. The machinery, parts and equipment all adopt conventional models in the existing technology, and the circuit connection adopts the conventional connection method in the existing technology, which will not be described in detail here. The control method of the present invention is controlled by a controller, and the control circuit of the controller can be implemented by simple programming by technicians in this field. The content not described in detail in this specification belongs to the existing technology known to professional and technical personnel in this field.

[0022] In the description of the present invention, it should be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0023] Working principle: When using the oocyte collector, the negative pressure tube 3 is first connected to an external negative pressure source, the flushing tube 20 is connected to the flushing liquid supply device, and the optical fiber light guide 13 is connected to an external cold light source. The collection tube 1 is sealed with a rubber plug 2 to ensure that there is no leakage in each tube. The doctor holds the handle 7 and quickly confirms the needle tip direction of the egg retrieval needle 8 through the surface pointing ridge 11. Combined with the tungsten alloy ultrasonic imaging coating 9 on the distal end of the egg retrieval needle 8 (clear imaging under ultrasound) and the optical fiber light guide 13 (transmitting real-time images of the interior of the follicle), the target follicle is accurately located. During collection, the rotary control switch 14 on the handle 7 is rotated to switch to the suction path through the reversing valve, and the pressing block 15 on the outer wall of the handle 7 is pressed to drive the internal regulating piston 18 to move and adjust the negative pressure of the suction path (fine-tuned according to the size of the follicle). After the egg retrieval needle 8 penetrates the follicle, the oocyte enters the suction tube 6 along with the follicular fluid through the egg retrieval needle 8, and finally flows into the collection tube 1 from the discharge head 10 at the end of the suction tube 6 (close to the inner wall of the collection tube 1), avoiding direct impact and causing cell damage; If the follicles need to be flushed to improve the recovery rate, the rotary control switch 14 is switched to the flushing path. The flushing liquid enters the egg retrieval needle 8 through the flushing tube 20 and the reversing valve. After flushing the follicles, it is switched to the suction path again to suck the flushed oocytes and liquid into the collection tube 1. The suction head 4 at one end of the negative pressure tube 3 has a built-in baffle 5 to prevent the oocytes from being directly sucked into the negative pressure tube 3 by the negative pressure, thereby ensuring the collection efficiency. After the operation is completed, the collection tube 1 is removed and the oocytes can be subsequently processed.

[0024] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An oocyte collector, comprising a collection tube (1) and a rubber plug (2) inserted into the collection tube (1), characterized in that: Two mounting holes are provided on the surface of the rubber plug (2), and a negative pressure tube (3) and a suction tube (6) are fixed in the two mounting holes respectively. A handle (7) is fixed to the outside of the suction tube (6), and an egg retrieval needle (8) is inserted into one end of the handle (7). An ultrasonic development coating (9) is provided on the end of the egg retrieval needle (8) away from the handle (7). A fixing ring (12) is fixed to the outer wall of one side of the handle (7). A plurality of fixing holes are provided at corresponding positions of the handle (7) and the fixing ring (12), and an optical fiber light guide (13) is installed in the fixing hole.

2. An oocyte collector according to claim 1, characterized in that: An air suction head (4) is installed at one end of the negative pressure pipe (3) located inside the collecting pipe (1), and a baffle (5) is fixed inside the air suction head (4).

3. The oocyte collector according to claim 1, characterized in that: A discharge head (10) is fixed to one end of the suction tube (6) located inside the collection tube (1), and the discharge end of the discharge head (10) is close to the inner wall of the collection tube (1).

4. The oocyte collector according to claim 1, characterized in that: A flushing tube (20) is installed at one end of the handle (7), and a reversing valve is installed at the connection between the suction tube (6) and the flushing tube (20). The suction tube (6) and the flushing tube (20) respectively cooperate with the reversing valve to form a suction passage and a flushing passage respectively. A rotary control switch (14) for controlling the reversing valve is installed on one side outer wall of the handle (7).

5. The oocyte collector according to claim 1, characterized in that: A movable hole is formed on the outer wall of the handle (7), and a pressing block (15) is movably connected in the movable hole. A fixing ring (16) is fixed to the outer wall of the pressing block (15), and an elastic pad (17) is sleeved on the outside of the pressing block (15).

6. The oocyte collector according to claim 5, characterized in that: An adjusting piston (18) is fixed to the end surface of the pressing block (15) located inside the handle (7), and a limiting ring (19) movably connected to the handle (7) is fixed to the outside of the adjusting piston (18).

7. The oocyte collector according to claim 1, characterized in that: The ultrasonic development coating (9) is made of tungsten alloy and is distributed at equal intervals along the axial direction of the egg retrieval needle (8).

8. The oocyte collector according to claim 1, characterized in that: The surface of the handle (7) is provided with a pointing ridge (11) which is parallel to the axis of the egg retrieval needle (8).

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