Formulations and compositions for inhibiting skin darkness or preventing wrinkle formation

Abstract

The present invention provides a preparation which can use silver skin as a functional material for cosmetic effect-related applications. The preparation contains an extract derived from silver skin as an active ingredient, and is intended at least to inhibit skin darkness or prevent wrinkle formation. Wherein the preparation contains an elastin inhibitor component derived from a silver skin extract, and is used for preventing the formation of wrinkles. In addition, the preparation contains at least one of a glycosylation inhibitor component derived from a silver skin extract, a carbonylation inhibitor component of a protein, a nitration inhibitor component of a protein, and a nitrotyrosine decomposer component, and is used for inhibiting darkness.

Description

TECHNICAL FIELD

[0001] The present application relates to effective use of silver skin, and particularly to a preparation that can at least inhibit dullness of skin and prevent wrinkle formation. BACKGROUND

[0002] Silver skin (mass-produced at coffee roasting plants) refers to a thin skin that covers coffee beans. The fruit of coffee is botanically referred to as a drupe, as shown in Figure 6 The coffee fruit is composed of an exocarp, mesocarp, pectin (mucilage), parchment (endocarp), silver skin, and seed, in order from the outside. The seed contains an embryo and endosperm, which are arranged opposite to each other in one coffee fruit. As the coffee fruit matures, the endosperm side plane, which faces each other, is gradually recessed and folded into the endosperm, and a groove called a raphe is formed at the center of the plane portion.

[0003] Coffee beans from which the exocarp portion of the seed is removed are green beans, and the process of extracting green beans from the coffee fruit is referred to as polishing. In the polishing process of coffee green beans, part of the silver skin is removed, and the remaining silver skin is mostly removed during roasting and is usually disposed as industrial waste.

[0004] In the past, part of the silver skin has been used as a material for the production of allulose (see Patent Document 1), paper (see Patent Document 2), company envelopes, business cards, greeting cards, wrapping paper, toys, and the like.

[0005] In addition, it is known that silver skin is effective as a functional material such as a hyaluronidase inhibitor (see Patent Document 3 and Non-Patent Document 1).

[0006]

Patent Document

[0007]

Patent Document 1

[0008]

Patent Document 2

[0009]

Patent Document 3

[0010]

Non-Patent Document

[0011]

Non-Patent Document 1

[0012] Technical problem to be solved by the invention

[0013] The inventors of this invention conducted extensive research to find more effective uses for silver bark, traditionally treated primarily as industrial waste. They discovered that in addition to using silver bark as a hyaluronidase inhibitor, it can also be used to inhibit skin dullness and prevent wrinkle formation. Silver bark is a natural product, highly safe for human consumption, and can be applied or ingested daily.

[0014] In view of the above circumstances, an object of the present invention is to provide a preparation that can use silver peel as a functional material for applications related to cosmetic effects.

[0015] The preparation of the present invention can at least inhibit skin dullness or prevent wrinkle formation, and its active ingredient is an extract from silver bark. In other words, the preparation of the present invention can be used as a preparation for inhibiting skin dullness, a preparation for preventing wrinkle formation, or a preparation for both.

[0016] As used herein, silverskin refers to the thin skin that surrounds the endosperm of plants in the Rubiaceae family (e.g., Coffea or Mascaro), such as coffee beans. This skin is produced in large quantities in coffee roasting plants. Silverskin can be obtained from both green and roasted coffee beans.

[0017] The silver skin of coffee beans contains components such as caffeine, 5-hydroxymethylfurfural, and chlorogenic acid. The caffeine content of roasted silver skin ranges from approximately 0.8 to 1.4 g / 100 g. The silver skin of coffee beans is primarily composed of dietary fiber (60-80%), consisting of cellulose, hemicellulose, and lignin. The main monosaccharides found in the silver skin are xylose, arabinose, rhamnose, fucose, mannose, glucose, galactose, and uronic acid (a derivative of oxidized monosaccharides).

[0018] The protein content of coffee beans' silver skin is approximately 18%, with lower levels of other nutrients such as fat and reducing sugars. Furthermore, the fat content is approximately 7.5g per 100g, with the fatty acids primarily being saturated (64%), followed by polyunsaturated (30%) and monounsaturated (6%). Furthermore, the silver skin contains phenolic compounds, which have a high antioxidant effect.

[0019] The present invention discloses a preparation containing a silver bark extract containing an elastase inhibitor, which can be used to prevent wrinkle formation. This preparation has the cosmetic benefits of preventing wrinkles, improving skin elasticity, and combating aging. Alternatively, the silver bark extract can be used as an elastase inhibitor.

[0020] Furthermore, the preparation of the present invention includes a silver bark extract containing a glycation inhibitor, which can be used to inhibit skin dullness. This preparation has the cosmetic effects of inhibiting skin dullness, improving skin elasticity, and combating aging. From another perspective, the silver bark extract can be used as a glycation inhibitor.

[0021] Additionally, the preparation of the present invention includes a silver bark extract containing a protein carbonylation inhibitor, which can be used to inhibit skin dullness. Protein carbonylation is a protein denaturation reaction that reduces skin transparency. By inhibiting carbonylation, the preparation has cosmetic benefits such as inhibiting skin dullness, improving skin elasticity, and providing anti-aging benefits. From another perspective, the silver bark extract can be used as a protein carbonylation inhibitor.

[0022] In addition, the preparation of the present invention includes a silver bark extract containing a protein nitration inhibitor, which can be used to inhibit skin dullness. Protein nitration can cause yellowing and dullness in the skin. By inhibiting nitration, this preparation has cosmetic benefits such as inhibiting skin dullness, improving skin elasticity, and anti-aging. From another perspective, the silver bark extract can be used as a protein nitration inhibitor.

[0023] In addition, the preparation of the present invention includes a silver bark extract containing a nitrotyrosine decomposition agent, which can be used to inhibit skin dullness. Nitrotyrosine (3-nitrotyrosine) is a nitrated modification of protein, produced by the nitration of tyrosine residues by reactive nitrogen species such as nitric oxide, nitrogen dioxide, and peroxynitrite. Nitrotyrosine can cause yellowing and dullness of the skin, and this preparation can decompose nitrotyrosine, thereby having cosmetic effects such as inhibiting skin dullness, improving skin elasticity, and anti-aging. From another perspective, the extract from silver bark can be used as a nitrotyrosine decomposition agent.

[0024] In the preparation of the present invention, the amount of the extraction solvent is preferably 10 to 20 times the amount of the silver bark extract. Furthermore, the content of the silver bark extract in the preparation of the present invention is preferably 1000 ppm (0.1%) or higher. In this specification, the units of % and ppm are by mass.

[0025] The preparation of the present invention can be used as an anti-aging agent. By inhibiting skin dullness or preventing wrinkle formation, it has a skin-beautifying and cosmetic effect, and can be used as an anti-aging agent to maintain a youthful and healthier appearance than one's actual age. Furthermore, the preparation has the efficacy of reducing disease risk and extending healthy lifespan.

[0026] The preparation of the present invention can be used as a cosmetic composition for suppressing skin dullness and preventing wrinkle formation, as a food composition for use as a food, as a pharmaceutical composition for use as a pharmaceutical, and as a quasi-drug composition for use as a quasi-drug.

[0027] In any of the methods of the present invention for suppressing skin dullness or preventing wrinkle formation, the preparation of the present invention can be used as an oral agent or a skin application agent.

[0028] Effects of the Invention

[0029] According to the present invention, silver skin can be effectively utilized as a functional material, is highly safe for the human body, and has the effects of inhibiting skin dullness and preventing wrinkle formation. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a comparison curve of glycosylation inhibition rate

[0031] Figure 2 Comparison of carbonylation inhibition rates

[0032] Figure 3 Comparison chart of nitrification inhibition rate

[0033] Figure 4 This is a comparison chart of the decomposition rate of nitrotyrosine

[0034] Figure 5 This is a comparison chart of the inhibition rate of elastase activity

[0035] Figure 6 This is the illustration of the silver skin DETAILED DESCRIPTION

[0036] (Silverbark Extraction Steps)

[0037] Extracts were prepared from the silverskin obtained from roasted coffee beans and their effectiveness on the skin was evaluated.

[0038] The extraction method was as follows: Silver bark was weighed and placed in three beakers. Distilled water was added to the beakers at 10, 20, and 30 times the amount of silver bark, respectively. Each mixture was then heated at 60°C for 4 hours and filtered using filter paper and a membrane filter to obtain an extract. Each extract solution was concentrated using an evaporator and then freeze-dried to obtain a dry product (extract).

[0039] The extraction results are shown in Table 1. When the amount of the extraction solvent is small (10 times), it is difficult to obtain the extraction solution without pressing because most of the added solvent is absorbed by the silver skin. The results shown in Table 1 (1) are obtained after pressing extraction, but the yield of the extraction solution is only 43%, which is small. On the other hand, as shown in Table 1 (2), when the amount of the extraction solvent is 20 times or more, there is no problem in operability as in the case of obtaining an extraction solution from other ordinary plants, and the yield of the extraction solution is 66.5%. In addition, as shown in Table 1 (3), the yield of the extraction solution is also improved (43%→66.5%→78%) by increasing the amount of the solvent. However, as the yield of the extraction solution is improved, the ratio of solid matter in the extraction solution decreases (1.53%→0.77%→0.54%). Therefore, for the subsequent test, the extract (dry matter) obtained by extraction with 20 times the amount of distilled water shown in Table 1 (2) was used.

[0040] [Table 1]

[0041] Solvent ratio relative to raw materials Yield relative to the amount of added solvent (%) Solid matter ratio in extract (%) (1) ×10 (10 times the amount) 43.0 1.53 (2) ×20 (20 times the amount) 66.5 0.77 (3) ×30 (30 times the amount) 78.0 0.54

[0042] As described above, it was found that when the extract was obtained from the silver skin, the yield of the extraction solution was low (less than 50%) and there was a problem in operability when the ratio of the solvent to the silver skin (raw material) was low (10 times, for example). However, when the amount of the extraction solvent was 20 times or more, the extract could be obtained smoothly.

[0043] Since the silver skin has a strong water absorption property, if 10 times the amount of water is added (water amount is 10 times), the water is absorbed by the silver skin, and it is difficult to extract without pressing. Therefore, there are problems in extraction time and yield if a solid-liquid separation method such as natural sedimentation (decantation) and gravity filtration is performed. On the other hand, even when 10 times the amount of water is added (water amount is 10 times), by performing a pressing operation such as compression, centrifugal separation, or vacuum filtration without performing solid-liquid separation, the extraction time can be shortened and the yield can be improved.

[0044] Therefore, when the amount of the extraction solvent is 20 times or more, the extract can be obtained smoothly. However, when the amount of the extraction solvent is 20 times or more, the concentration process requires more cost and time. Therefore, it is also a good choice to perform extraction at a low water addition ratio.

[0045] [Example 1]

[0046] (Regarding the Glycosylation Inhibition Test)

[0047] Evaluation results showing whether the silver skin extract has a glycosylation inhibition effect are shown.

[0048] It is well known that glycosylation in the skin is associated with various skin aging processes, and the glycosylation of skin proteins and the accumulation of advanced glycation end products (AGEs: advanced glycation end products) due to age growth or glycosylation stress cause the skin tone to become yellowish, resulting in dullness. Since the half-life of proteins such as collagen or elastin, which are the main components of the dermis, is long, they are easily affected by glycosylation. Therefore, glycosylation of proteins in the dermis layer has an important influence on maintaining the stability of fibrous tissue and can cause a decrease in skin firmness and elasticity.

[0049] Therefore, inhibition of glycosylation is an effective method for maintaining skin health.

[0050] The test sample was silver skin extract, and aminoguanidine hydrochloride was used as a positive control. The silver skin extract was dissolved in distilled water so that the final concentration of the silver skin extract could be adjusted to 100 ppm or 1000 ppm in the test system to prepare a sample solution. In addition, the final concentration of aminoguanidine hydrochloride (positive control) was 40 mM.

[0051] Regarding the test method, D-glucose, D-ribose, and bovine serum albumin were each dissolved in 1 M phosphate buffer so that the final concentration was 10%, 1%, and 1%, respectively. The sample solution was mixed in this solution so that the final concentration of the silver skin extract was 100 ppm and 1000 ppm, respectively, and incubated at 60°C for 3 days. The control group was replaced with a phosphate buffer instead of the sample solution. After the reaction was completed, 200 μL of each reaction solution was dispensed into a 96-well plate, and the fluorescence intensity at 465 nm upon excitation at 360 nm was measured by a fluorescence microplate reader. The amount of advanced glycation end products produced by the reaction was calculated, and based on the amount of advanced glycation end products of the control group, the glycosylation inhibition rate of the silver skin extract at each concentration was calculated.

[0052] Figure 1 The test results of the glycosylation inhibition test are illustrated. When 100 ppm and 1000 ppm of silver skin (CS: coffee silver skin) extract was added, respectively, no glycosylation inhibition was confirmed at 100 ppm, but glycosylation inhibition was confirmed at 1000 ppm.

[0053] From the above results, it can be confirmed that the silver skin extract has a glycosylation inhibition effect at a prescribed concentration or higher.

[0054]

Example 2

[0055] (Regarding the carbonylation inhibition test)

[0056] The following are the evaluation results of whether the silver skin extract has an inhibition effect on carbonylation.

[0057] Since aldehydes such as acrolein, which is a degradation product of lipid peroxide, are attached to proteins to carbonylate the proteins, it is known that many carbonylated proteins exist in the stratum corneum, epidermis and dermis tissues of the skin portion exposed to light.

[0058] Carbonylation in the skin is associated with various factors, such as yellowing of dermal proteins caused by carbonylation, reduction in optical transparency in the stratum corneum caused by carbonylated proteins after sunlight irradiation, and dryness of the skin caused by carbonylation of keratin in the stratum corneum. Therefore, inhibition of carbonylation in the skin is effective for maintaining healthy skin.

[0059] The test sample was a silver bark extract, and aminoguanidine hydrochloride was used as a positive control. The silver bark extract was dissolved in distilled water so that the final concentration of the silver bark extract could be adjusted to 100 ppm or 1000 ppm in the test system to prepare a sample solution. In addition, the final concentration of aminoguanidine hydrochloride was 40 mM.

[0060] As to the test method, a 96-well plate coated with Cellmatrix (registered trademark) Type I-A collagen was prepared, and 100 μL of the sample solution was dispensed into each well so that the final concentrations of 0.5 mM acrolein and the silver bark extract were 100 ppm and 1000 ppm, respectively.

[0061] In addition, distilled water was added to the wells of the control group. The number n of wells for each sample was set to 6 wells. After reacting for one day at 37°C, the reaction solution was removed, and the plate was washed with distilled water, 200 μL of 20 μM fluorescein-5-aminothiourea / 0.1 M MES-Na (pH 5.5) was added, and the plate was stained at room temperature. After staining, the staining solution was removed, the plate was washed with T-PBS, and the fluorescence intensity at 535 nm under excitation at 465 nm was measured by a fluorescence microplate reader. The amount of carbonylation produced by the reaction was calculated, and the carbonylation inhibition rate of the silver bark extract at each concentration was calculated based on the amount of carbonylation of the control group.

[0062] The test results of the carbonylation inhibition test are shown in Table 1. Figure 2 When the silver bark (CS) extract was added at 100 ppm and 1000 ppm, respectively, no carbonylation inhibition was observed at 100 ppm, but weak carbonylation inhibition was confirmed at 1000 ppm.

[0063] From the above results, it was confirmed that the silver bark extract has a carbonylation inhibition effect at a prescribed concentration or higher.

[0064] [Example 3]

[0065] (Regarding nitration inhibition test)

[0066] The following are the results of an evaluation of whether the silver bark extract has an inhibitory effect on nitrification.

[0067] Nitration is a post-translational modification of proteins in which reactive nitrogen species produced in the body impart nitro groups to the benzene rings of tyrosine or tryptophan residues that constitute proteins. Protein nitration is known to affect cellular function by impairing the function of enzymes and tyrosine kinase receptors. Accumulation of nitrotyrosine in proteins is associated with numerous diseases, including arteriosclerosis and ischemic brain disease. Furthermore, protein nitration is implicated in a wide range of diseases and symptoms in various tissues within the body. Furthermore, studies have shown that protein nitration can cause yellowing of the skin, and that nitration of stratum corneum cell adhesion factors in the stratum corneum is associated with poor stratum corneum exfoliation. Therefore, preventing skin nitration is crucial.

[0068] The test sample was an extract of Aralia argentea, and ferulic acid was used as a positive control. The sample solution was prepared by dissolving the Aralia argentea extract in distilled water to adjust the final concentration of Aralia argentea extract to 100 ppm or 1000 ppm in the test system. The final concentration of ferulic acid was 3 mM.

[0069] Regarding the test method, the tyrosine solution was dispensed into 1.5 mL test tubes, and the sample solution was added and mixed to give final concentrations of the silver bark extract of 100 ppm and 1000 ppm, respectively. Simultaneously, distilled water was added to the control group instead of the sample. Peroxynitrite was added to a concentration of 100 μM, and the resulting mixture was incubated at 37°C for 24 hours. The reaction solution was then filtered through a filter, and the amount of nitrotyrosine generated by the reaction was quantitatively analyzed by high-performance liquid chromatography (HPLC). The amount of nitrotyrosine generated by the reaction was calculated, and the carbonylation inhibition rate of the silver bark extract at each concentration was calculated based on the amount of nitrotyrosine in the control group.

[0070] The results of the nitrification inhibition test were as follows Figure 3 When 100 ppm and 1000 ppm of the silver bark (CS) extract were added, almost no nitrification inhibitory effect was observed at 100 ppm, but a strong nitrification inhibitory effect was observed at 1000 ppm.

[0071] According to the above results, the silver bark extract has a strong inhibitory effect on nitrogenation at a specified concentration or higher.

[0072] [Example 4]

[0073] (About nitro compound decomposition test)

[0074] The following are the results of an evaluation of whether the silver bark extract has the ability to decompose nitro compounds (here, nitrotyrosine).

[0075] Research has shown that the adverse effects of nitration in the skin, particularly yellowing and dulling of the skin, are caused not only by glycation and carbonylation of proteins in the dermis, but also by nitrated proteins formed by nitration of amino acids that make up proteins in the stratum corneum. Therefore, if the nitrated proteins produced by nitration can be broken down, there is hope for improving the various symptoms caused by protein nitration in the skin, such as yellowing and dullness.

[0076] The test sample was an extract of Aralia arvensis, and the positive control used sodium dithionite. The sample solution was prepared by dissolving the Aralia arvensis extract in distilled water to adjust the final concentration of Aralia arvensis extract to 100 ppm or 1000 ppm in the test system. The final concentration of sodium dithionite was 1 mM.

[0077] Regarding the test method, 25 μL of sample solution was added to each well of a 96-well plate to a final concentration of 100 ppm and 1000 ppm of silver bark extract. Then, 225 μL of a 0.005% aqueous nitrotyrosine solution was added, respectively. A control group used distilled water instead of sample. The 96-well plate was sealed and incubated at 37°C for 72 hours. After the reaction was completed, the absorbance at 450 nm was measured using a microplate reader. The ratio of the nitrotyrosine content after adding silver bark extract to the nitrotyrosine content in the control group was calculated, and the ratio of nitrotyrosine reduction was used as the nitrotyrosine degradation rate.

[0078] Figure 4 The results of the nitrate decomposition test are shown. When 100 ppm and 1000 ppm of silver bark (CS) extract were added, no decomposition of p-nitrotyrosine was observed at 100 ppm, but p-nitrotyrosine decomposition was observed at 1000 ppm.

[0079] The above results indicate that the silver bark extract has a decomposition effect on nitro compounds at the specified concentration or higher.

[0080] [Example 5]

[0081] (About Elastase Activity Inhibition Test)

[0082] The following are the results of an evaluation of whether the silver bark extract has an inhibitory effect on the enzymatic activity of elastase (ie, elastase).

[0083] It is well known that wrinkles develop due to long-term exposure to ultraviolet light, known as photoaging, and changes in the dermal matrix are generally considered a major factor. It is also known that in photoaged skin, changes in the elastic fiber elastin, a component of the dermal matrix, occur through an increase in normal fibers and thickening of the fibers, resulting in the formation of amorphous masses.

[0084] On the other hand, it is known that the activity of elastase, an enzyme that degrades elastin, increases significantly in skin irradiated with ultraviolet rays. Therefore, as a method for preventing wrinkles due to photoaging, it is important to inhibit the elastase activity that increases after ultraviolet irradiation.

[0085] The test sample was an extract of silver bark, and ethylenediaminetetraacetic acid (EDTA) was used as a positive control. The sample solution was prepared by dissolving the silver bark extract in distilled water to adjust the final concentration of silver bark extract to 100 ppm or 1000 ppm in the test system. The final concentration of EDTA was 250 μg.

[0086] Regarding the test method, 8 mM elastase substrate (methoxysuccinyl-alanine-alanine-propyl-valine-p-nitroaniline) was dispensed into each well of a 96-well plate, and the sample solution was added to achieve a final concentration of silver bark extract of 100 ppm and 1000 ppm. The control group used distilled water instead of the sample. Then, 0.25 units / mL of human leukocyte elastase was added and mixed, and the mixture was incubated at 37°C for 20 minutes. The absorbance at 405 nm was then measured using a microplate reader. Based on the absorbance of the absorbing substance produced by the decomposition of the elastase substrate, the amount of substrate decomposition by elastase in the control group and after the addition of silver bark extract was calculated. Furthermore, the ratio of the amount of substrate decomposition by elastase in the control group to the amount of substrate decomposition after the addition of silver bark extract was calculated, and the ratio of the reduced substrate decomposition amount was used as the elastase activity inhibition rate.

[0087] The results of the elastase activity inhibition test were as follows Figure 5 When 100 ppm and 1000 ppm of the silver bark (CS) extract were added, almost no inhibitory effect on elastase activity was observed at 100 ppm, but an inhibitory effect on elastase activity was observed at 1000 ppm.

[0088] These results indicate that the silver bark extract has an effect of inhibiting elastase activity at a predetermined concentration or higher.

[0089] (Cosmetic composition, food composition, pharmaceutical composition, quasi-drug composition)

[0090] 1) Cosmetic compositions can be appropriately selected depending on the intended use, and include, for example, lotions, cosmetic serums, creams, and emulsions. 2) Food compositions can be appropriately selected depending on the intended use, and include, for example, beverages such as coffee, black tea, soft drinks, carbonated beverages, nutritional beverages, fruit drinks, and lactic acid beverages, and foods such as candy, jelly, chewing gum, and chocolate.

[0091] 3) Pharmaceutical compositions can be appropriately selected according to the intended use, and include, for example, tablets, capsules, beverage preparations, lozenges, and the like.

[0092] 4) Quasi-drug compositions can be appropriately selected according to the intended use, and include, for example, medicated soaps, bath preparations, and the like.

[0093] Industrial Applicability

[0094] The present invention is useful as a cosmetic composition, food composition, etc. for suppressing skin dullness or preventing wrinkle formation.

Claims

1. A preparation whose active ingredient is an extract of silver bark, used to inhibit skin dullness or prevent wrinkle formation.

2. The preparation according to claim 1, wherein the extract contains an elastase inhibitor component for preventing wrinkle formation.

3. The preparation according to claim 1, wherein the extract contains a glycation inhibitor component for suppressing dullness.

4. The preparation according to claim 1, wherein the extract contains a protein carbonylation inhibitor component for suppressing dullness.

5. The preparation according to claim 1, wherein the extract contains a protein nitration inhibitor component for suppressing dullness.

6. The preparation according to claim 1, wherein the extract contains a nitrotyrosine decomposing agent component for suppressing dullness.

7. An elastase inhibitor, the active ingredient of which is derived from an extract of silver bark.

8. A glycation inhibitor, the active ingredient of which is derived from an extract of silver bark.

9. A protein carbonylation inhibitor, the active ingredient of which is derived from an extract of silver bark.

10. A protein nitration inhibitor, the active ingredient of which is derived from an extract of silver bark.

11. A nitrotyrosine decomposing agent, the active ingredient of which is derived from an extract of silver bark. 12 . The preparation according to claim 1 , wherein the amount of the extraction solvent is 10 to 20 times that of the extract.

13. The preparation according to any one of claims 1 to 11, wherein the extract is contained in an amount of 1000 ppm or more.

14. The preparation according to any one of claims 1 to 11, which is used as an anti-aging agent.

15. A cosmetic comprising the preparation according to any one of claims 1 to 11, which is used as a cosmetic composition.

16. A food comprising the preparation according to any one of claims 1 to 11, which is used as a food composition.

17. A pharmaceutical product comprising the preparation according to any one of claims 1 to 11, which is used as a pharmaceutical composition.

18. A quasi-drug comprising the preparation according to any one of claims 1 to 11, which is used as a quasi-drug composition.

19. A method for inhibiting skin dullness and preventing wrinkle formation, which comprises using the preparation according to any one of claims 1 to 11 as an oral agent.

20. A method for suppressing skin dullness and preventing wrinkle formation, the method comprising using the preparation according to any one of claims 1 to 11 as a skin coating agent.

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