Bacteriostatic and anti-inflammatory biological polysaccharide flushing fluid and preparation method thereof

Abstract

The invention discloses a bacteriostatic and anti-inflammatory biological polysaccharide flushing fluid and a preparation method of the bacteriostatic and anti-inflammatory biological polysaccharide flushing fluid, and belongs to the technical field of flushing fluids. Comprising the following raw materials: 1.5%-8.0% of sodium carboxymethyl cellulose, 0.5%-6.5% of carboxyethyl chitosan, 0.1%-3.5% of fructose oxide, 0.1%-2.0% of sodium chloride, 0.1%-1.0% of dipotassium glycyrrhizinate and the balance of purified water. The antibacterial and anti-inflammatory effects of the biological polysaccharide flushing fluid are further improved by adding carboxyethyl chitosan and fructose oxide, meanwhile, the invention discloses a preparation process of carboxyethyl chitosan and fructose oxide, the whole preparation process is simple and efficient, finally, dipotassium glycyrrhizinate has an excellent anti-inflammatory effect, and the dipotassium glycyrrhizinate is prepared according to the formula, so that the antibacterial and anti-inflammatory effects of the biological polysaccharide flushing fluid are improved. The finally prepared biological polysaccharide flushing fluid has excellent antibacterial and anti-inflammatory effects.

Description

Technical Field

[0001] This invention belongs to the field of rinsing solution technology, specifically relating to an antibacterial and anti-inflammatory biological polysaccharide rinsing solution and its preparation method. Background Technology

[0002] In modern surgical procedures, the treatment of traumatic wounds or surgical incisions often involves rinsing the wound or incision with saline solution. However, conventional saline solution can only provide a simple rinsing and cleaning effect on the wound or incision.

[0003] To address the issue of the limited functionality of saline solution, existing technologies have made numerous improvements. For example, Chinese patent CN105126159A discloses a polysaccharide biomedical colloidal solution and its preparation method. This invention patent adds sodium carboxymethyl cellulose with different viscosity gradients to a sodium chloride solution. Sodium carboxymethyl cellulose is a commonly used dressing ingredient with antibacterial properties. Furthermore, Chinese patent CN108939056A discloses a biopolysaccharide rinsing adhesive and its preparation method. This invention patent's rinsing adhesive formulation adds polyvinyl alcohol, hyaluronic acid, and carboxymethyl cellulase to further enhance its antibacterial properties. Even further, Chinese patent CN111760018A discloses a highly effective antibacterial and anti-inflammatory biopolysaccharide rinsing solution and its preparation method. This invention patent adds lysozyme to achieve antibacterial, anti-inflammatory, and healing-promoting effects.

[0004] While adding sodium carboxymethyl cellulose, carboxymethyl cellulase, lysozyme, and other technologies can enhance the functionality of existing wound / wound irrigation solutions, this remains a conventional approach. The present invention aims to develop a biological polysaccharide irrigation solution and its preparation method to further improve the antibacterial and anti-inflammatory effects of the irrigation solution. Summary of the Invention

[0005] The purpose of this invention is to improve the antibacterial and anti-inflammatory effects of biological polysaccharide rinsing solutions, so as to solve the technical problem that the existing wound / wound rinsing solutions have relatively limited functionality.

[0006] One object of the present invention is to provide an antibacterial and anti-inflammatory biological polysaccharide rinse solution;

[0007] Another object of the present invention is to provide a method for preparing the above-mentioned biological polysaccharide rinsing solution.

[0008] The objective of this invention can be achieved through the following technical solutions:

[0009] A bio-polysaccharide rinse solution with antibacterial and anti-inflammatory properties, comprising the following ingredients by weight percentage:

[0010] Sodium carboxymethyl cellulose 1.5%-8.0%;

[0011] Carboxyethyl chitosan 0.5%-6.5%;

[0012] Oxidized fructose: 0.1%-3.5%;

[0013] Sodium chloride 0.1%-2.0%;

[0014] Dipotassium glycyrrhizate 0.1%-1.0%;

[0015] The remainder is purified water.

[0016] Furthermore, the antibacterial and anti-inflammatory biopolysaccharide rinse solution comprises, by weight percentage, the following raw materials:

[0017] Sodium carboxymethyl cellulose 6.5%;

[0018] Carboxyethyl chitosan 2.5%;

[0019] Oxidized fructose 1.2%;

[0020] Sodium chloride 0.9%;

[0021] Dipotassium glycyrrhizate 0.4%;

[0022] The remainder is purified water.

[0023] Further, the carboxyethyl chitosan is prepared by the following steps:

[0024] Chitosan was stirred in deionized water at room temperature for 1 hour to obtain a chitosan suspension. Acrylic acid was added to the chitosan suspension and the mixture was magnetically stirred at 50°C for 3 days. After stirring, 1 mol / L NaOH solution was added to adjust the pH to 10.0-12.0. The mixture was then purified by dialysis with pure water for 3 days and dried at room temperature to obtain carboxyethyl chitosan.

[0025] Furthermore, the ratio of deionized water, chitosan, and acrylic acid is 200mL:4-5g:5.8-6.2mL.

[0026] Furthermore, the oxidized fructose is prepared by the following steps:

[0027] Pectin was added to deionized water at room temperature and stirred for 1 hour to obtain a pectin suspension. Sodium periodate was added to the pectin suspension and the mixture was magnetically stirred for 12 days in the dark at room temperature. Ethylene glycol was then added and stirred for 30 minutes. The mixture was then purified by dialysis with pure water for 2 days and dried at room temperature to obtain oxidized fructose.

[0028] Furthermore, the ratio of deionized water, pectin, sodium periodate, and ethylene glycol is 100mL:3.2-3.5g:3.2-3.5g:4.5-5.0mL.

[0029] Furthermore, the preparation method of the biological polysaccharide rinsing solution includes the following steps:

[0030] Weigh the above raw materials according to the mass percentage, add sodium carboxymethyl cellulose, carboxyethyl chitosan, oxidized fructose, sodium chloride and dipotassium glycyrrhizate to purified water to obtain a mixed solution, stir the mixed solution at a speed of 1000 r / min for 1 h, let it stand for 2 h, filter it with a cellulose acetate ultrafiltration membrane to obtain a clear solution, fill the clear solution and place it in a 120℃ high-temperature steam sterilizer for sterilization, after sterilization, the biopolysaccharide rinsing solution is obtained.

[0031] Furthermore, the cellulose acetate ultrafiltration membrane has a pore size of 3 μm.

[0032] The beneficial effects of this invention are:

[0033] This invention provides a formulation for a biopolysaccharide flushing solution, comprising sodium carboxymethyl cellulose, carboxyethyl chitosan, oxidized fructose, sodium chloride, dipotassium glycyrrhizate, and purified water. Building upon existing technologies, this invention enhances the antibacterial and anti-inflammatory effects of the biopolysaccharide flushing solution by adding carboxyethyl chitosan and oxidized fructose. Furthermore, this invention discloses the preparation process of carboxyethyl chitosan and oxidized fructose, resulting in a simple and efficient overall preparation process. Finally, dipotassium glycyrrhizate possesses excellent anti-inflammatory properties; when prepared according to the formulation of this invention, the final biopolysaccharide flushing solution exhibits superior antibacterial and anti-inflammatory effects. Detailed Implementation

[0034] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1

[0036] Preparation of carboxyethyl chitosan:

[0037] At room temperature, 4 g of chitosan (degree of deacetylation ≥ 95%; viscosity 100-200 mPa·s; Shanghai Maclean) was added to 200 mL of deionized water and stirred for 1 h to obtain a chitosan suspension. 5.8 mL of acrylic acid was added to the chitosan suspension and the mixture was magnetically stirred at 50 °C for 3 days. After the stirring was completed, 1 mol / L NaOH solution was added to adjust the pH to 10.0. The mixture was then purified by dialysis with pure water for 3 days and dried at room temperature to obtain carboxyethyl chitosan.

[0038] Example 2

[0039] Preparation of carboxyethyl chitosan:

[0040] At room temperature, 4.5 g of chitosan (degree of deacetylation ≥ 95%; viscosity 100-200 mPa·s; Shanghai Maclean) was added to 200 mL of deionized water and stirred for 1 h to obtain a chitosan suspension. 6.0 mL of acrylic acid was added to the chitosan suspension and the mixture was magnetically stirred at 50 °C for 3 days. After stirring, 1 mol / L NaOH solution was added to adjust the pH to 11.0. The mixture was then purified by dialysis with pure water for 3 days and dried at room temperature to obtain carboxyethyl chitosan.

[0041] Example 3

[0042] Preparation of carboxyethyl chitosan:

[0043] At room temperature, 5g of chitosan (degree of deacetylation ≥95%; viscosity 100-200mpa.s; Shanghai Maclean) was added to 200mL of deionized water and stirred for 1h to obtain a chitosan suspension. 6.2mL of acrylic acid was added to the chitosan suspension and magnetically stirred at 50℃ for 3d. After completion, 1mol / L NaOH solution was added to adjust the pH to 12.0, and then purified by dialyzing with pure water for 3d. After drying at room temperature, carboxyethyl chitosan was obtained.

[0044] Example 4

[0045] Preparation of oxidized fructose:

[0046] At room temperature, 3.2 g of pectin (Shanghai Maclean) was added to 100 mL of deionized water and stirred for 1 h to obtain a pectin suspension. 3.2 g of sodium periodate was added to the pectin suspension and the mixture was magnetically stirred for 12 days in the dark at room temperature. Then, 4.5 mL of ethylene glycol was added and stirred for 30 min. The mixture was then purified by dialysis with pure water for 2 days and dried at room temperature to obtain oxidized fructose.

[0047] Example 5

[0048] Preparation of oxidized fructose:

[0049] At room temperature, 3.3 g of pectin (Shanghai Maclean) was added to 100 mL of deionized water and stirred for 1 h to obtain a pectin suspension. 3.4 g of sodium periodate was added to the pectin suspension and the mixture was magnetically stirred for 12 days in the dark at room temperature. Then, 4.8 mL of ethylene glycol was added and stirred for 30 min. The mixture was then purified by dialysis with pure water for 2 days and dried at room temperature to obtain oxidized fructose.

[0050] Example 6

[0051] Preparation of oxidized fructose:

[0052] At room temperature, 3.5 g of pectin (Shanghai Maclean) was added to 100 mL of deionized water and stirred for 1 h to obtain a pectin suspension. 3.5 g of sodium periodate was added to the pectin suspension and the mixture was magnetically stirred for 12 days in the dark at room temperature. Then, 5.0 mL of ethylene glycol was added and stirred for 30 min. The mixture was then purified by dialyzing with pure water for 2 days and dried at room temperature to obtain oxidized fructose.

[0053] Example 7

[0054] Preparation of antibacterial and anti-inflammatory biological polysaccharide rinse solution:

[0055] First, the above-mentioned biopolysaccharide rinsing solution comprises the following raw materials by weight percentage:

[0056] Sodium carboxymethyl cellulose 1.5%;

[0057] 0.5% of carboxyethyl chitosan prepared in Example 1;

[0058] Example 4 prepared 0.1% oxidized fructose;

[0059] Sodium chloride 0.1%;

[0060] Dipotassium glycyrrhizate 0.1%;

[0061] The remainder is purified water.

[0062] Then, the preparation method of the above-mentioned biological polysaccharide rinsing solution includes the following steps:

[0063] Weigh the above raw materials according to the mass percentage, add sodium carboxymethyl cellulose (viscosity 1500-3100 mpa.s; Shanghai Maclean), carboxyethyl chitosan prepared in Example 1, oxidized fructose prepared in Example 4, sodium chloride and dipotassium glycyrrhizate to purified water to obtain a mixed solution. Stir the mixed solution at a rate of 1000 r / min for 1 h. After the stirring is completed, let it stand for 2 h. Filter it with a cellulose acetate ultrafiltration membrane (pore size 3 μm) to obtain a clear solution. After filling the clear solution, place it in a high-temperature steam sterilizer at 120℃ for sterilization. After sterilization, the biopolysaccharide rinsing solution is obtained.

[0064] Example 8

[0065] Preparation of antibacterial and anti-inflammatory biological polysaccharide rinse solution:

[0066] First, the above-mentioned biopolysaccharide rinsing solution comprises the following raw materials by weight percentage:

[0067] Sodium carboxymethyl cellulose 6.5%;

[0068] 2.5% of carboxyethyl chitosan prepared in Example 2;

[0069] The oxidized fructose prepared in Example 5 was 1.2%;

[0070] Sodium chloride 0.9%;

[0071] Dipotassium glycyrrhizate 0.4%;

[0072] The remainder is purified water.

[0073] Then, the preparation method of the above-mentioned biological polysaccharide rinsing solution includes the following steps:

[0074] Weigh the above raw materials according to the mass percentage, add sodium carboxymethyl cellulose (viscosity 1500-3100 mpa.s; Shanghai Maclean), carboxyethyl chitosan prepared in Example 2, oxidized fructose prepared in Example 5, sodium chloride and dipotassium glycyrrhizate to purified water to obtain a mixed solution. Stir the mixed solution at a rate of 1000 r / min for 1 h. After the stirring is completed, let it stand for 2 h. Filter it with a cellulose acetate ultrafiltration membrane (pore size 3 μm) to obtain a clear solution. After filling the clear solution, place it in a high-temperature steam sterilizer at 120℃ for sterilization. After sterilization, the biopolysaccharide rinsing solution is obtained.

[0075] Example 9

[0076] Preparation of antibacterial and anti-inflammatory biological polysaccharide rinse solution:

[0077] First, the above-mentioned biopolysaccharide rinsing solution comprises the following raw materials by weight percentage:

[0078] Sodium carboxymethyl cellulose 8.0%;

[0079] 6.5% of carboxyethyl chitosan was prepared in Example 3;

[0080] The oxidized fructose prepared in Example 6 was 3.5%;

[0081] Sodium chloride 2.0%;

[0082] Dipotassium glycyrrhizate 1.0%;

[0083] The remainder is purified water.

[0084] Then, the preparation method of the above-mentioned biological polysaccharide rinsing solution includes the following steps:

[0085] Weigh the above raw materials according to the mass percentage, add sodium carboxymethyl cellulose (viscosity 1500-3100 mpa.s; Shanghai Maclean), carboxyethyl chitosan prepared in Example 3, oxidized fructose prepared in Example 6, sodium chloride and dipotassium glycyrrhizate to purified water to obtain a mixed solution. Stir the mixed solution at a rate of 1000 r / min for 1 h. After the stirring is completed, let it stand for 2 h. Filter it with a cellulose acetate ultrafiltration membrane (pore size 3 μm) to obtain a clear solution. After filling the clear solution, place it in a high-temperature steam sterilizer at 120℃ for sterilization. After sterilization, the biopolysaccharide rinsing solution is obtained.

[0086] Comparative Example 1

[0087] Comparative Example 1 served as the control group for Example 8. The carboxyethyl chitosan prepared in Example 2 of Example 8 was removed, while the remaining raw materials and preparation methods remained unchanged from those in Example 8, ultimately yielding a biopolysaccharide rinsing solution.

[0088] Comparative Example 2

[0089] Comparative Example 2 served as the control group for Example 8. The oxidized fructose prepared in Example 5 of Example 8 was removed, while the remaining raw materials and preparation methods remained unchanged from those in Example 8, ultimately yielding a biopolysaccharide rinsing solution.

[0090] Comparative Example 3

[0091] Comparative Example 3 served as the control group for Example 8. The carboxyethyl chitosan prepared in Example 2 and the oxidized fructose prepared in Example 5 of Example 8 were removed, while the remaining raw materials and preparation methods remained unchanged from those in Example 8, ultimately yielding a biopolysaccharide rinsing solution.

[0092] The performance of the biopolysaccharide rinsing solutions prepared in Examples 7-9 and Comparative Examples 1-3 was tested. The test process is as follows, and the test results are shown in Table 1.

[0093] Antibacterial performance testing:

[0094] To prepare bacterial suspensions, Escherichia coli (Gram-negative bacillus) and Staphylococcus aureus (Gram-positive cocci) were selected to study the antibacterial properties of the biopolysaccharide rinsing solution. First, the original solutions of Escherichia coli and Staphylococcus aureus were added dropwise to 20 mL of sterilized nutrient broth, and then the solution was placed in a constant temperature shaker (100 rpm, 37 °C) and cultured for 24 h to obtain bacterial suspensions of Escherichia coli and Staphylococcus aureus.

[0095] Escherichia coli and Staphylococcus aureus were mixed with culture medium to prepare identification culture medium. After solidification, holes were punched with a sterile 5 mm diameter punch. 50 μL of biological polysaccharide washing solution was dropped into the center of each hole. The medium was incubated at 37°C for 24 h, and the size of the inhibition zone was observed. The results are shown in Table 1 below.

[0096] Table 1

[0097]

[0098] As can be seen from Table 1, the biological polysaccharide rinsing solution prepared in this invention has excellent antibacterial properties.

[0099] It should be noted that, in this document, terms such as “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0100] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A biological polysaccharide rinse solution with antibacterial and anti-inflammatory properties, characterized in that, By weight percentage, it includes the following raw materials: Sodium carboxymethyl cellulose 1.5%-8.0%; Carboxyethyl chitosan 0.5%-6.5%; Oxidized fructose: 0.1%-3.5%; Sodium chloride 0.1%-2.0%; Dipotassium glycyrrhizate 0.1%-1.0%; The remainder is purified water.

2. The antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 1, characterized in that, The antibacterial and anti-inflammatory biopolysaccharide rinse solution comprises, by weight percentage, the following raw materials: Sodium carboxymethyl cellulose 6.5%; Carboxyethyl chitosan 2.5%; Oxidized fructose 1.2%; Sodium chloride 0.9%; Dipotassium glycyrrhizate 0.4%; The remainder is purified water.

3. The antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 1, characterized in that, The carboxyethyl chitosan is prepared by the following steps: Chitosan was stirred in deionized water at room temperature for 1 hour to obtain a chitosan suspension. Acrylic acid was added to the chitosan suspension and the mixture was magnetically stirred at 50°C for 3 days. After stirring, 1 mol / L NaOH solution was added to adjust the pH to 10.0-12.

0. The mixture was then purified by dialysis with pure water for 3 days and dried at room temperature to obtain carboxyethyl chitosan.

4. The antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 3, characterized in that, The ratio of deionized water, chitosan, and acrylic acid is 200mL:4-5g:5.8-6.2mL.

5. The antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 1, characterized in that, The oxidized fructose is prepared by the following steps: Pectin was added to deionized water at room temperature and stirred for 1 hour to obtain a pectin suspension. Sodium periodate was added to the pectin suspension and the mixture was magnetically stirred for 12 days in the dark at room temperature. Ethylene glycol was then added and stirred for 30 minutes. The mixture was then purified by dialysis with pure water for 2 days and dried at room temperature to obtain oxidized fructose.

6. The antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 5, characterized in that, The ratio of deionized water, pectin, sodium periodate, and ethylene glycol is 100mL:3.2-3.5g:3.2-3.5g:4.5-5.0mL.

7. The method for preparing an antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 1, characterized in that, Includes the following steps: Weigh the above raw materials according to the mass percentage, add sodium carboxymethyl cellulose, carboxyethyl chitosan, oxidized fructose, sodium chloride and dipotassium glycyrrhizate to purified water to obtain a mixed solution, stir the mixed solution at a speed of 1000 r / min for 1 h, let it stand for 2 h, filter it with a cellulose acetate ultrafiltration membrane to obtain a clear solution, fill the clear solution and place it in a 120℃ high-temperature steam sterilizer for sterilization, after sterilization, the biopolysaccharide rinsing solution is obtained.

8. The method for preparing an antibacterial and anti-inflammatory biological polysaccharide rinse solution according to claim 7, characterized in that, The cellulose acetate ultrafiltration membrane has a pore size of 3 μm.

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