An ectopic ossification arthritis animal model and a construction method and application thereof

Abstract

The application relates to the technical field of animal models, and discloses a construction method and application of an ectopic ossification arthritis animal model. 91‑115 After being mixed and emulsified with chicken type II collagen as an antigen, animals are immunized to obtain the animal model, which is referred to as a C2V7 model. The arthritis model constructed by the application has high success rate, short time consumption and easily-obtained modeling materials. The model can simulate the onset process of arthritis with obvious ectopic ossification characteristics such as ankylosing spondylitis, enthesial-related arthritis and psoriatic arthritis, objectively reflects the pathological process and pathological characteristics of ectopic ossification arthritis, and provides a new research platform for related research.

Claims

1. A method for constructing an animal model of heterotopic ossifying arthritis, characterized in that, This includes the peptide VG1 from chondroitin sulfate proteoglycan. 91-115 As an antigen, it was mixed with type II collagen and emulsified before being used to immunize animals; the polypeptide VG1 91 -115 The amino acid sequence is shown in SEQ ID NO: 1; the animal is a BALB / c mouse or a DBA / 1 mouse.

2. The method according to claim 1, characterized in that, include: Synthetic peptide VG1 91-115 ; The polypeptide VG1 91 -115 The mixture was mixed with chicken type II collagen solution, then emulsified with complete Freund's adjuvant to form a water-in-oil emulsion; the emulsion was then subcutaneously injected into the tail of mice.

3. The method according to claim 2, characterized in that, include: Chemically synthesized peptide VG1 91-115 VG1 91-115 Mix the emulsion with 2 mg / mL chicken type II collagen solution and 4 mg / mL complete Freund's adjuvant at a 1:1 ratio. Place the mixture in ice water and homogenize at 8000 rpm for 2 minutes, pause for 2 minutes, and repeat the homogenization process 15-20 times to form an emulsion that does not disperse when dropped into water. Administer 100 μL of the emulsion per mouse to BALB / c or DBA / 1 mice by inserting a needle 2 cm from the base of the tail until the needle tip is 0.5 cm from the tail base. Inject the emulsion intraperitoneally with 200 μL of 15 mg / mL fungal polysaccharide Curdlan on the day of injection and again 7 days later.

4. The method according to claim 1, characterized in that, The fungal polysaccharide Curdlan was injected intraperitoneally into the animals on the day of immunization and one week later.

5. The use of the animal model of heterotopic ossifying arthritis obtained by the method of any one of claims 1-4 in screening or identifying compounds for treating or alleviating heterotopic ossifying arthritis, or in studying the disease mechanism of heterotopic ossifying arthritis.

6. A method for screening candidate drugs or therapeutic agents to relieve or treat heterotopic ossifying arthritis, characterized in that, include: An animal model of heterotopic ossifying arthritis is obtained by the method according to any one of claims 1-4; the candidate drug or therapeutic agent is administered to the animal model, and if the arthritis symptoms of the animal model are relieved, the candidate drug or therapeutic agent is used as an active ingredient for relieving or treating heterotopic ossifying arthritis.

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