Method for extracting pine nut oil from Siberia Korean pine nuts

Abstract

A method for extracting pine nut oil from Siberia Korean pine nuts is characterized in that the Siberia Korean pine nuts serve as raw materials and are smashed, a solvent and enzyme are added into the pine nuts according to a certain liquid-material ratio, the mixture is added into a homogenizer and filtered after ultrasonic extraction and incubation, after filtering, the solvent is recycled through a rotary evaporator under reduced pressure till no solvent flows out, then the temperature is increased to completely remove the solvent, and the pine nut oil is obtained. The pine nut oil is obtained.

Description

Technical Field:

[0001] This invention relates to the field of natural product chemistry, and in particular to a method for extracting pine nut oil from Siberian red pine nuts. Background technology:

[0002] Pine nuts are the seeds of pine trees (Pinaceae family). Pine nuts are a naturally occurring substance, with fatty acids comprising over 50% of their content. They contain over 60% oil, higher than oilseeds such as peanuts, soybeans, and sesame seeds. Pine nuts have high nutritional value, containing various types of unsaturated fatty acids, including linolenic acid, linoleic acid, and oleic acid, as well as the unique component pinolenic acid. Pinolenic acid is an isomer of gamma-linolenic acid (GLA), a novel polyunsaturated fatty acid belonging to the Δ5 type of polyunsaturated fatty acids, and possesses functions such as weight loss, lowering blood lipids, and inhibiting cancer cell metastasis.

[0003] Pine nuts contain 24%–30% oil, which is rich in beneficial components such as gamma-linolenic acid, making it highly valuable. Pine nut oil has various physiological functions, such as weight loss and lipid-lowering effects.

[0004] Korean Patent KR20190033335(A) relates to a method for producing pine seed oil using supercritical fluid. This method uses supercritical fluid instead of organic solvents to extract oil from pine seeds, thereby increasing the content of unsaturated fatty acids in the oil and thus improving its anti-inflammatory and moisturizing effects on the skin. The disadvantage of this method is that the experimental operation is difficult to control and is relatively complex.

[0005] Korean Patent KR102484807(B1) discloses a method for manufacturing pine nut oil, which includes a stage in the preparation of pine nuts, a stage in the extraction of pine nut oil from pulverized pine nuts using supercritical fluid extraction, and a stage in the extraction of triglycerides from pine nut oil containing German compounds, specifically a coating stage. This method is complex.

[0006] Japanese Patent HK1064700(A1) describes a method for effectively producing pine nut oil from pine nuts, comprising the following steps: First, pine nuts are crushed, and pine kernels and crushed pine shells are mixed at a volume ratio of 60±20% and 40±20%; then, they are placed in an oil press at 70-100 degrees Celsius for oil extraction. Additionally, a plate heating device with temperature control is installed between the oil press shaft and the outside of the conveyor section to assist in the oil extraction. The pine nut oil obtained by this method contains some pine nut shell components.

[0007] Chinese patent CN101050398 describes a method for extracting pine nut oil from pine nuts using an oil press. The extracted pine nuts are mixed with screened and washed rice husks, wheat husks, or small pieces of rice straw or wheat stalks cut to a certain length, in a specific volume ratio, and then fed into an oil press. The extracted pine nut oil is then allowed to settle naturally to obtain pine nut oil. However, the pine nut oil obtained by this method is not pure enough.

[0008] Chinese patent CN1465682 discloses a processing method for pine nut oil by supercritical carbon dioxide extraction. It involves processing pine nuts from red pine or dwarf pine through crushing, drying, supercritical carbon dioxide extraction, and separation. The content of unsaturated fatty acids can reach 70-90%. The disadvantage of this method is that the separation and purification technology is simplistic and lacks novelty.

[0009] Chinese patent CN109287769A discloses a method for producing pine nut oil. The method involves crushing and grinding pine nuts into a paste, then mixing egg whites from black-shelled chickens. The egg whites are added to the pine nut paste at 40-50 degrees Celsius. After 5 minutes, the egg whites bind with the protein molecules in the pine nut paste, thus separating the oil molecules. The separated pine nut oil is then heated to remove moisture, filtered, and sterilized to obtain pine nut oil. This method uses black-shelled chicken egg whites, and the separation process is relatively complex.

[0010] Chinese patent CN1132607C discloses a Yunnan pine nut extract, which can be prepared by the following method: Yunnan pine nuts are used as raw materials, screened, dried, repeatedly pressed with an oil press, filtered, and then deacidified in hot alkaline water at 70℃ to achieve an acid value of 0.8%-1.5%. The extract is then washed with hot water at 70℃-90℃ and dehydrated under vacuum to obtain the Yunnan pine nut oil extract. The drawback of this method is that the purity of the pine nut oil is not high enough.

[0011] In summary, current methods for extracting pine nut oil mainly utilize oil presses and supercritical fluid extraction. This method has the following drawbacks: complex process and delicate procedures; low purity of the crude product; and some solvents used may cause contamination. Summary of the Invention:

[0012] The purpose of this invention is to provide a method for extracting pine nut oil from Siberian red pine nuts.

[0013] To achieve the above objectives, the technical solution adopted in this invention includes: using Siberian red pine nuts as raw material, crushing them, adding solvent and enzyme to the pine nuts at a certain liquid-to-material ratio, adding them to a homogenizer, ultrasonically extracting and incubating them, filtering them, and then using a rotary evaporator to recover the solvent under reduced pressure until no solvent flows out, and then heating them to completely remove the solvent to obtain pine nut oil.

[0014] The advantages of this invention are:

[0015] 1. Made with Siberian red pine nuts, ensuring the purity of the pine nut oil.

[0016] 2. This invention changes the traditional production process by using a portion of enzymes to replace the traditional solvent as the extraction solvent. The raw materials do not need to be dried and can be used directly, resulting in low energy consumption, high pine nut oil yield, and avoidance of pollution.

[0017] 3. Extraction using a homogenizer saves on operating procedures and solvent usage, resulting in low production costs and high yield.

[0018] 4. All solvents are recyclable, facilitating large-scale industrial production. Detailed implementation method:

[0019] The embodiments of the present invention will be described in further detail below:

[0020] Using Siberian red pine nuts as raw material, the pine nuts are crushed, and solvent and enzyme are added to the pine nuts at a certain liquid-to-solid ratio. The mixture is then added to a homogenizer, ultrasonically extracted and incubated, and then filtered. The solvent after filtration is recovered by a rotary evaporator under reduced pressure until no solvent flows out. The solvent is then completely removed by heating to obtain pine nut oil.

[0021] The present invention will now be further described with reference to embodiments, but it is not limited to any one of these embodiments or similar examples.

[0022] Example 1:

[0023] Add 2% by weight of 1:1:1 (w / w) cellulase, pectinase, and hemicellulase to 10g of pine nuts; add 5 times the weight of pure water to adjust the pH to 3; homogenize in a homogenizer at 5000 rpm for 2 minutes; incubate at 45℃ under 100W ultrasonic power for 2 hours. 6g of pine nut oil is obtained.

[0024] Example 2:

[0025] Add 3% by weight (w / w) of 1:1:1 (w / w) cellulase, pectinase, and hemicellulase to 10g of pine nuts; add 20 times the weight of pure water to adjust the pH to 7; homogenize in a homogenizer at 25,000 rpm for 4 minutes; incubate at 65℃ under 200W ultrasonic power for 3 hours. 6.7g of pine nut oil was obtained.

Claims

1. A method for extracting pine nut oil from Siberian red pine nuts, characterized in that: Using Siberian red pine nuts as raw material, the pine nuts are crushed, and solvent and enzyme are added to the pine nuts at a certain liquid-to-solid ratio. The mixture is then added to a homogenizer, ultrasonically extracted and incubated, and then filtered. The solvent after filtration is recovered by a rotary evaporator under reduced pressure until no solvent flows out. The solvent is then completely removed by heating to obtain pine nut oil.

2. The method for extracting pine nut oil from Siberian red pine nuts according to claim 1, characterized in that: Siberian red pine kernels contain 1.5%-5% by weight of 1:1:1 (w / w) cellulase, pectinase and hemicellulase.

3. The method for extracting pine nut oil from Siberian red pine nuts according to claim 1, characterized in that: Add 5-20 times the weight of pine nuts in pure water to adjust the pH to 3-7.

4. The method for extracting pine nut oil from Siberian red pine nuts according to claim 1, characterized in that: Homogenize in a homogenizer at a speed of 5000-25000 rpm for 2-6 minutes.

5. The method for extracting pine nut oil from Siberian red pine nuts according to claim 1, characterized in that: Incubate at 45-65℃ for 1-6 hours under ultrasonic power of 100-600W.