Nutrient solution capable of increasing vitamin C of plants and preparation method of nutrient solution

Abstract

The invention discloses a nutrient solution capable of increasing vitamin C of plants and a preparation method of the nutrient solution, and relates to the technical field of plant nutrition and fertilizers. The nutrient solution contains additives such as 2-keto-L-gulonic acid or 2-keto-L-gulonic acid salt and the like. According to the invention, 2-keto-L-gulonic acid or 2-keto-L-gulonic acid sodium or 2-keto-L-gulonic acid potassium and the like are used as core raw materials of the nutrient solution for plants, and are compounded with basic nutrient raw materials and additives required by the plants to prepare the plant nutrient solution and the composite nutrient solution. The 2-keto-L-gulonic acid and the 2-keto-L-gulonate in the plant body can be catalyzed and converted into the vitamin C, so that the content of the vitamin C in the tree body is remarkably increased.

Description

Technical Field

[0001] This invention relates to the field of plant nutrition and fertilizer technology, specifically to a nutrient solution for increasing vitamin C in plants and its preparation method. Background Technology

[0002] Woody plants experience significant nutrient depletion during long-distance or long-term transplantation, leading to weakened root regeneration and reduced survival rates. Furthermore, woody plants are susceptible to weakening, aging, and disease during their growth and development, requiring continuous conditioning and protection in terms of nutrition and pest control. Injectable plant nutrient solutions can rapidly replenish the nutrients needed by plants, resulting in quick effects and a huge market demand.

[0003] However, currently available injectable plant nutrient solutions mainly provide plants with nitrogen, phosphorus, potassium, trace elements, and phytostimulants, and no injectable plant nutrient solutions have been found that can increase the vitamin C content of plants.

[0004] Vitamin C is widely present in plants. It is a highly active antioxidant with multiple functions, such as: participating in the removal of reactive oxygen species; maintaining the reduced state of vitamin E to protect plants from oxidative stress; participating in the synthesis of coenzymes, chloroplasts, and electron donors or acceptors in the plasma membrane; participating in the biosynthesis of oxalic acid and tartaric acid; participating in cell elongation and division; and participating in the biosynthesis of ethylene and gibberellin.

[0005] Through stable isotope tracing, metabolomics, and genomics, we discovered that 2-keto-L-gulonic acid, after being absorbed by plants, can be converted into vitamin C as a precursor. Therefore, injecting phytonutrients containing 2-keto-L-gulonic acid increases the vitamin C content in plants, thereby significantly improving their stress resistance and growth.

[0006] 2-Keto-L-gulonic acid is an intermediate in the industrial fermentation of vitamin C, derived from sorbitol via a two-step bio-fermentation process. Therefore, this technology can obtain sufficient, high-purity 2-keto-L-gulonic acid, sodium 2-keto-L-gulonic acid, or potassium 2-keto-L-gulonic acid for the preparation of a novel plant nutrient solution that enhances vitamin C levels in plants. Furthermore, industrial vitamin C production generates a residual liquid containing a large amount of 2-keto-L-gulonic acid—gulonic acid mother liquor. Previously, this residual liquid required environmentally friendly treatment, incurring high processing costs and wasting resources. To address this, we propose a new technology for formulating and preparing a plant nutrient solution that enhances vitamin C levels in plants by secondary processing of the gulonic acid mother liquor and compounding it with basic nutrient solution raw materials and additives. Summary of the Invention

[0007] The purpose of this invention is to provide a nutrient solution for increasing vitamin C in plants and its preparation method, in order to solve the problems mentioned in the background art, such as the large amount of nutrient consumption in woody plants during long-distance or long-interval transplantation, the weakening of root regeneration ability, and the difficulty in survival; in addition, woody plants are prone to problems such as weakness, aging, and disease during growth and development, and need to be injected with plant nutrient solution to quickly replenish the nutrients required by the plants, and to continuously regulate and protect them, thereby improving the survival rate and growth status.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A nutrient solution for enhancing vitamin C in plants, the nutrient solution containing 2-keto-L-gulonic acid or a salt additive obtained by the conversion of 2-keto-L-gulonic acid.

[0010] Preferably, the nutrient solution includes a gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36%.

[0011] Preferably, the salt additives contained in the nutrient solution are sodium 2-keto-L-gulonic acid or potassium 2-keto-L-gulonic acid crystals obtained by sodium or potassium salt transformation of 2-keto-L-gulonic acid and final concentration and crystallization.

[0012] Preferably, the 2-keto-L-gulonic acid is obtained by adsorbing impurities and decolorizing gulonic acid mother liquor with activated carbon.

[0013] Preferably, the gulonic acid mother liquor is a gulonic acid mother liquor containing 2-keto-L-gulonic acid obtained from the fermentation mash of vitamin C industrial production after ultrafiltration separation, ion exchange, concentration and crystallization processes.

[0014] A compound nutrient solution for enhancing vitamin C in plants, wherein the compound nutrient solution is prepared by mixing nutrient solution and basic nutrient solution raw materials in a ratio of 1:0.1-30.

[0015] Preferably, the basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, and the balance being water.

[0016] This invention also provides a method for preparing a compound nutrient solution that enhances vitamin C in plants, comprising the following steps:

[0017] Step 1: Crush large clumps of nutrient solution raw materials by mechanical means, and then screen them through a 50-mesh sieve to leave only the nutrient solution raw materials that meet the specifications and are easy to dissolve.

[0018] Step 2: Thoroughly mix 2-keto-L-gulonic acid, sodium 2-keto-L-gulonic acid, potassium 2-keto-L-gulonic acid crystals, or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% with the screened basic nutrient solution raw material obtained in Step 1 to obtain a preliminary mixture;

[0019] Step 3: Place the preliminary mixture obtained in Step 2, along with additives (such as indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, sodium dodecylbenzenesulfonate, etc.) and sterile water into a liquid mixer and mix thoroughly for 10 to 60 minutes to obtain a semi-finished mixture.

[0020] Step 4: After pasteurizing the semi-finished mixture from Step 3, fill it using a filling machine, and then seal it to obtain the finished nutrient solution.

[0021] Preferably, the finished nutrient solution needs to be diluted with water before use, and the dilution ratio is 1:10-400. The amount of finished nutrient solution to be injected varies depending on the circumference of the seedling. Specifically, when the circumference of the seedling is 1-60 cm, the required amount of finished nutrient solution is 100 ml to 1000 ml per injection.

[0022] Preferably, the storage tank for the finished nutrient solution needs to be made of an opaque material, and the sealing material needs to be corrosion-resistant.

[0023] Compared with existing technologies, one or more of the above technical solutions have the following beneficial effects:

[0024] 1. This invention uses 2-keto-L-gulonic acid, sodium 2-keto-L-gulonic acid, or potassium 2-keto-L-gulonic acid as raw materials for plant nutrient solutions. 2-keto-L-gulonic acid can be catalyzed and converted into vitamin C in plants, thereby significantly increasing the vitamin C content in trees.

[0025] 2. This invention uses waste gulonic acid mother liquor as a raw material for plant nutrient solution, which not only solves the problem of resource utilization of gulonic acid mother liquor, but also provides high-quality raw materials for the production of plant nutrient solution, thus realizing the sustainable utilization of resources.

[0026] 3. The nutrient solution containing 2-keto-L-gulonic acid used in this invention has the ability to improve the antioxidant capacity of plants, such as resisting ozone and ultraviolet rays.

[0027] 4. The nutrient solution containing 2-keto-L-gulonic acid used in this invention has the effect of promoting plant root growth, extending the taproot, and increasing capillary roots.

[0028] 5. The nutrient solution containing 2-keto-L-gulonic acid used in this invention provides plants with a small molecule organic carbon source, which can improve plant photosynthesis and be directly absorbed and utilized by plants. It participates in the primary metabolism (sugars, proteins and fats) and secondary metabolism (oxalic acid, tartaric acid, ethylene and gibberellin, etc.) of plants, thereby improving plant survival rate and promoting plant growth. Attached Figure Description

[0029] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0030] In the attached diagram:

[0031] Figure 1 This is a schematic diagram showing the effect of nutrient solutions in each group on the vitamin C content of different plant seedlings in Experiments 1 and 2. Detailed Implementation

[0032] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0033] 2-Keto-L-gulonic acid can be transformed into sodium carbonate and finally concentrated and crystallized to obtain sodium 2-keto-L-gulonic acid crystals, with a sodium 2-keto-L-gulonic acid content of over 90%.

[0034] The main component of gulonic acid mother liquor is 2-keto-L-gulonic acid, with a content as high as 15-35%.

[0035] 2-Keto-L-gulonic acid is a precursor for vitamin C synthesis, but it cannot spontaneously form vitamin C at room temperature in the absence of a catalyst.

[0036] Example 1

[0037] A compound nutrient solution to enhance vitamin C in plants is prepared by first adding 2-keto-L-gulonic acid to a basic nutrient solution, and then adding additives and sterile water to obtain the finished nutrient solution.

[0038] The additives include indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, and sodium dodecylbenzenesulfonate;

[0039] The basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, and the remainder is water.

[0040] Example 2

[0041] A compound nutrient solution to enhance vitamin C in plants is prepared by first adding potassium 2-keto-L-gulonate crystals to a basic nutrient solution, and then adding additives and sterile water to obtain the finished nutrient solution.

[0042] The additives include indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, and sodium dodecylbenzenesulfonate;

[0043] The basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, and the remainder is water.

[0044] Example 3

[0045] A compound nutrient solution for enhancing vitamin C in plants is prepared by first adding gulonic acid stock solution containing 10% 2-keto-L-gulonic acid to a basic nutrient solution, and then adding additives and sterile water to obtain the finished nutrient solution.

[0046] The additives include indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, and sodium dodecylbenzenesulfonate;

[0047] The basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, and the remainder is water.

[0048] Example 4

[0049] A compound nutrient solution to enhance vitamin C in plants is prepared by first adding gulonic acid stock solution containing 20% ​​2-keto-L-gulonic acid to a basic nutrient solution, and then adding additives and sterile water to obtain the finished nutrient solution.

[0050] The additives include indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, and sodium dodecylbenzenesulfonate;

[0051] The basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, and the remainder is water.

[0052] Example 5

[0053] A compound nutrient solution for enhancing vitamin C in plants is prepared by first adding gulonic acid stock solution containing 36% 2-keto-L-gulonic acid to a basic nutrient solution, and then adding additives and sterile water to obtain the finished nutrient solution.

[0054] The additives include indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, and sodium dodecylbenzenesulfonate;

[0055] The basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, and the remainder is water.

[0056] Example 6

[0057] The method for preparing the compound nutrient solution for enhancing plant vitamin C in the above embodiments includes the following steps:

[0058] Step 1: Crush large clumps of nutrient solution raw materials by mechanical means, and then screen them through a 50-mesh sieve to leave only the nutrient solution raw materials that meet the specifications and are easy to dissolve.

[0059] Step 2: Thoroughly mix 2-keto-L-gulonic acid, sodium 2-keto-L-gulonic acid, potassium 2-keto-L-gulonic acid crystals, or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% with the screened basic nutrient solution raw material obtained in Step 1 to obtain a preliminary mixture;

[0060] Step 3: Place the preliminary mixture obtained in Step 2, along with additives (such as indoleacetic acid, gibberellin, deacetylated chitosan, oligochitosan, sodium dodecylbenzenesulfonate) and sterile water into a liquid mixer and mix thoroughly for 10 to 60 minutes to obtain a semi-finished mixture.

[0061] Step 4: After pasteurizing the semi-finished mixture from Step 3, fill it using a filling machine, and then seal it to obtain the finished nutrient solution.

[0062] When using the compound nutrient solution, the finished nutrient solution needs to be diluted with water at a ratio of 1:10 to 400. The amount of finished nutrient solution to be injected varies depending on the circumference of the seedling. Specifically, when the circumference of the seedling is 1 to 60 cm, the required amount of finished nutrient solution is 100 ml to 1000 ml per injection. The storage container for the finished nutrient solution needs to be made of opaque material, and the sealing material needs to be corrosion-resistant.

[0063] Experimental Example 1: The effect of compound nutrient solution on small plant seedlings (crabapple).

[0064] Control group 1

[0065] The compound nutrient solution was processed strictly according to the method in Example 6, but 2-keto-L-gulonic acid or sodium 2-keto-L-gulonic acid crystals or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% were not added in step two.

[0066] Experimental group 1

[0067] The compound nutrient solution was processed strictly according to the method in Example 6, that is, in step two, 2-keto-L-gulonic acid or sodium 2-keto-L-gulonic acid crystals or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% was added.

[0068] Reference Group 1

[0069] The compound nutrient solution was processed strictly according to the method in Example 6, but in step two, the 2-keto-L-gulonic acid or sodium 2-keto-L-gulonic acid crystals or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% was replaced with an equal amount of vitamin C as in the experimental group.

[0070] The experiment group (control group 1), experimental group 1, and reference group 1 each had five replicates, with one small plant seedling placed in each replicate. After three weeks of cultivation in an indoor greenhouse system, the plant nutrient solution of this patent was applied for three consecutive days. After a one-week break, 100g of tree leaves were collected, and the vitamin C content of the leaves from the control group, experimental group 1, and reference group 1 was measured. After a ten-week break, the growth indicators of the seedlings from the control group, experimental group 1, and reference group 1 were measured.

[0071] Table 1. Effects of nutrient solutions in each group on the growth performance of small plant seedlings (crabapple) in Experiment 1

[0072]

[0073] Combining Table 1 and Figure 1The results showed that, compared with control group 1, the nutrient solution for increasing plant vitamin C in experimental group 1, which used 2-keto-L-gulonic acid, sodium 2-keto-L-gulonic acid, potassium 2-keto-L-gulonic acid crystals, or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36%, significantly improved the growth performance of small plant seedlings, effectively increased the vitamin C content in small plant seedlings, and enhanced their growth and survival ability.

[0074] Experimental Example 2

[0075] The effects of a nutrient solution that enhances vitamin C levels in plants on medium-sized cherry seedlings, wherein:

[0076] Control group 2 strictly followed the method of any one of Examples 1-5 to process the nutrient solution, but did not add 2-keto-L-gulonic acid or sodium 2-keto-L-gulonic acid crystals or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% to the formula;

[0077] Experimental group 2 strictly followed the method of any one of Examples 1-5 to process the nutrient solution, that is, added 2-keto-L-gulonic acid or sodium 2-keto-L-gulonic acid crystals or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% to the basic nutrient solution raw materials.

[0078] Reference Group 2 strictly followed the method of any one of Examples 1-5 to process the nutrient solution, but replaced the 2-keto-L-gulonic acid or sodium 2-keto-L-gulonic acid crystals or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% with an equal amount of vitamin C as in Experimental Group 2.

[0079] Control group 2, experimental group 2, and reference group 2 each had three replicates, with one medium-sized plant seedling placed in each replicate. After 5 weeks of cultivation in an indoor greenhouse system, the plant nutrient solution of this patent was applied for 3 consecutive days. After a 1-week break, 100g of leaves were collected from the seedlings in control group 2, experimental group 2, and reference group 2 to determine the vitamin C content. After a 10-week break, the growth indicators of the seedlings in control group 2, experimental group 2, and reference group 2 were measured.

[0080] Table 2. Effects of nutrient solutions in each group on the growth performance of medium-sized cherry seedlings in Experiment 2.

[0081]

[0082] Combine Table 2 and Figure 1The results showed that, compared with control group 2, the nutrient solution for increasing plant vitamin C in experimental group 2, which used sodium 2-keto-L-gulonate crystals as an additive, significantly improved the growth performance of large plant seedlings, effectively increased the vitamin C content in the large plant seedlings, and enhanced their growth and survival ability.

[0083] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A nutrient solution for enhancing vitamin C in plants, characterized in that: The nutrient solution contains 2-keto-L-gulonic acid or a salt additive obtained by the conversion of 2-keto-L-gulonic acid.

2. The nutrient solution for enhancing vitamin C in plants according to claim 1, characterized in that: The nutrient solution includes a gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36%.

3. The nutrient solution for enhancing vitamin C in plants according to claim 1, characterized in that: The salt additives contained in the nutrient solution are sodium 2-keto-L-gulonic acid, potassium 2-keto-L-gulonic acid crystals, or 2-keto-L-gulonic acid salt solutions obtained by sodium or potassium salt transformation of 2-keto-L-gulonic acid and final concentration and crystallization.

4. The nutrient solution for enhancing vitamin C in plants according to claim 1, characterized in that: The 2-keto-L-gulonic acid can also be obtained by adsorbing impurities and decolorizing gulonic acid mother liquor with activated carbon.

5. A nutrient solution for enhancing plant vitamin C according to claim 2 or 4, characterized in that: The gulonic acid mother liquor is a gulonic acid mother liquor containing 2-keto-L-gulonic acid obtained from the fermentation mash of vitamin C industrial production through ultrafiltration separation, ion exchange, concentration and crystallization processes.

6. A compound nutrient solution for enhancing vitamin C in plants, characterized in that: The compound nutrient solution is prepared by mixing the nutrient solution according to any one of claims 1 to 5 with the basic nutrient solution raw materials in a ratio of 1:0.1 to 30.

7. The compound nutrient solution for enhancing plant vitamin C according to claim 6, characterized in that: The basic nutrient solution ingredients, by weight percentage, are: urea 0-10%, ferrous sulfate 1-9%, ammonium molybdate 0.1-2%, ammonium dihydrogen phosphate 0-6%, sodium nitrate 0.1-5%, ammonium sulfate 0.1-7%, potassium chloride 0.1-5%, potassium carbonate 0.1-5%, citric acid 0-3%, sodium alkylbenzene sulfonate 0-5%, with the remainder being water.

8. The method for preparing a compound nutrient solution for enhancing plant vitamin C according to claim 7, characterized in that, Includes the following steps: Step 1: Crush large clumps of nutrient solution raw materials by mechanical means, and then screen them through a 50-mesh sieve to leave only the nutrient solution raw materials that meet the specifications and are easy to dissolve. Step 2: Thoroughly mix 2-keto-L-gulonic acid, sodium 2-keto-L-gulonic acid, potassium 2-keto-L-gulonic acid crystals, or gulonic acid mother liquor with a 2-keto-L-gulonic acid content of 10-36% with the screened basic nutrient solution raw material obtained in Step 1 to obtain a preliminary mixture; Step 3: Place the preliminary mixture obtained in Step 2, along with the additives and sterile water, into a liquid mixer and mix thoroughly for 10 to 60 minutes to obtain a semi-finished mixture. Step 4: After pasteurizing the semi-finished mixture from Step 3, fill it using a filling machine, and then seal it to obtain the finished nutrient solution.

9. The application of the compound nutrient solution for enhancing plant vitamin C according to claim 7, characterized in that: The finished nutrient solution is diluted with water during use, with a dilution ratio of 1:10 to 400. The amount of finished nutrient solution injected varies depending on the circumference of the seedling. Specifically, when the circumference of the seedling is 1 to 60 cm, the required amount of finished nutrient solution is 100 ml to 1000 ml per injection.

10. The application of the compound nutrient solution for enhancing plant vitamin C according to claim 7, characterized in that: The storage tank for the finished nutrient solution is made of an opaque material, and the sealing material is corrosion-resistant.

Images