Composite microecological preparation and application thereof in feed

Abstract

The invention discloses a composite micro-ecological preparation and application thereof in feed, and belongs to the technical field of microorganisms, the composite micro-ecological preparation comprises lysinibacillus and mannan oligosaccharide, the lysinibacillus is preserved in the China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CCTCC No.M20241816. The invention further discloses a preparation method of the composite micro-ecological preparation. The compound microecological preparation disclosed by the invention can be used for more remarkably reducing the content of aflatoxin B1 and increasing the content of water-soluble polysaccharide, and is beneficial to increasing the market value of a feed containing peanut meal, corn and / or soybean meal.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a compound microecological preparation and its application in feed. Background Technology

[0002] In the applicant's earlier application CN2025106181719 (invention title: A Lysinibacillus macroides that degrades Alternaria toxins and its application), a Lysinibacillus macroides is described, which can effectively reduce and degrade Alternaria toxins. The Lysinibacillus macroides is deposited at the China Center for Type Culture Collection, located at Wuhan University, Hubei Province, with accession number CCTCC No. M20241816.

[0003] Peanut meal, corn and / or soybean meal are common feed ingredients, but they are all susceptible to aflatoxin contamination. Further research by the applicant revealed that this lysine-containing Bacillus can further reduce aflatoxin levels, thus completing this invention.

[0004] It should be noted that the entire contents of the prior application CN2025106181719 are incorporated into this invention and are part of the content of this invention. The applicant reserves the right to include the entire contents of the prior application CN2025106181719 in the specification of this invention and use it as the basis for modifications during subsequent examination. Summary of the Invention

[0005] Based on the above reasons, this invention proposes a compound microecological preparation and its application in feed, which has achieved a very beneficial effect in reducing aflatoxin B1. Specifically, in order to achieve the purpose of this invention, the following technical solution is proposed:

[0006] One aspect of this invention relates to a compound microecological preparation comprising Lysinibacillus macroides and mannan oligosaccharides, wherein the Lysinibacillus macroides is deposited at the China Center for Type Microbiology, located at Wuhan University, Hubei Province, with accession number CCTCC No. M20241816.

[0007] In a preferred embodiment of the present invention, the weight ratio of the lysine-containing Bacillus to the mannan oligosaccharide is 4-6:1; preferably 5:1.

[0008] In a preferred embodiment of the present invention, the compound microecological preparation further contains water.

[0009] Another aspect of the present invention relates to the application of the above-mentioned compound microecological preparation in reducing aflatoxin B1 in feed.

[0010] Another aspect of the present invention relates to a feed containing the above-mentioned compound microecological preparation as well as peanut meal and / or corn and / or soybean meal.

[0011] In a preferred embodiment of the present invention, the feed is obtained by fermenting peanut meal and / or corn and / or soybean meal with the above-mentioned compound microecological preparation.

[0012] Beneficial effects

[0013] The beneficial effects of this invention are: the compound microecological preparation of this invention can significantly reduce the content of aflatoxin B1 and increase the content of water-soluble polysaccharides, which helps to increase the market value of feed. Detailed Implementation

[0014] To further understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0015] Unless otherwise specified, all reagents involved in the embodiments of this invention are commercially available products and can be purchased through commercial channels.

[0016] Example 1:

[0017] 1. Isolation and preservation of Lysinibacillus macroides:

[0018] The inventors of this application discovered that Alternaria toxin was never detected in the intestines of turbot from some of the fish raised at the Yantai Laizhou Aquaculture Experimental Farm. Through further research, a strain of Lysinibacillus macroides was isolated from the intestines of the aforementioned fish and named LY015. This microbial strain is deposited at the China Center for Type Culture Collection (CCTCC), located at Wuhan University, Hubei Province, with accession number CCTCCNo.M20241816 and deposit date of August 19, 2024.

[0019] 2. Strain identification

[0020] Genomic DNA was extracted using the TIANGEN bacterial genomic DNA extraction kit. Using the genomic DNA of the test strain as a template, the target fragment was amplified using the universal primers 27F / 1492R for the bacterial 16S rRNA gene. The PCR products were detected by 1% agarose gel electrophoresis, and the PCR stock solution was sent to Bomeide Biotechnology Co., Ltd. for sequencing. 16S rRNA gene sequence analysis, after sequence comparison and identification, confirmed that strain LY015 is *Lysinibacillus macroides* using MK757660.1 (NCBI) as the standard. The 16S rDNA gene sequence is shown in SEQ ID NO. 1.

[0021]

[0022] 3. Preparation of compound microecological preparations

[0023] 500g of Bacillus lysine LY015 fermentation broth (containing 10g of Bacillus lysine LY015) 8 Add 10g of mannan oligosaccharide to (CFU / g, dry matter content of 10%) and mix evenly to obtain a compound microecological preparation.

[0024] As a control, the fermentation broth of Bacillus lysine-containing LY015 was used directly.

[0025] 4. Detection of the effect of compound microecological preparations in reducing aflatoxin B1 in peanut meal feed.

[0026] (1) Add aflatoxin B1 to peanut meal, with aflatoxin B1 content of 300 μg / kg;

[0027] (2) Mix 50g of compound microecological preparation and LY015 fermentation liquid as control with 950g of peanut meal, and then add 500g of sterilized water.

[0028] (3) After fermentation at 30℃ for 3 days, the content of aflatoxin B1 was detected, and the changes in water-soluble polysaccharides were also detected.

[0029] Table 1. Effects of compound microecological preparations on peanut meal feed

[0030]

[0031] 5. Detection of the effect of compound microecological preparations in reducing aflatoxin B1 in plant protein feed.

[0032] (1) Preparation of compound plant protein feed: peanut meal: corn: soybean meal are mixed evenly in a weight ratio of 1:1:2;

[0033] (2) Add aflatoxin B1 to the above plant protein feed, with aflatoxin B1 content of 300 μg / kg;

[0034] (3) Mix 50g of compound microecological preparation and LY015 fermentation liquid as control with 950g of plant protein feed respectively, and then add 500g of sterilized water.

[0035] (4) After fermentation at 30℃ for 3 days, the content of aflatoxin B1 was detected, and the changes in water-soluble polysaccharides were also detected.

[0036] Table 2. Effects of compound microecological preparations on plant protein feed

[0037]

[0038] The above experimental results indicate that although Bacillus lysine LY015 alone can reduce the content of aflatoxin B1 and increase the content of water-soluble polysaccharides, the compound microecological preparation combined with mannan oligosaccharides can more significantly reduce the content of aflatoxin B1 and increase the content of water-soluble polysaccharides, which helps to increase the market value of feed containing peanut meal and / or corn and / or soybean meal.

[0039] The preferred embodiments of the present invention have been described above, but are not intended to limit the invention. Those skilled in the art can make modifications and variations to the embodiments disclosed herein without departing from the scope and spirit of the invention.

Claims

1. A compound microecological preparation, characterized in that: It includes lysinibacillus macroides and mannan oligosaccharides. The lysinibacillus macroides is deposited at the China Center for Type Microbiology, located at Wuhan University, Hubei Province, with accession number CCTCC No. M20241816.

2. The compound microecological preparation according to claim 1, characterized in that: The weight ratio of the lysine-containing Bacillus to the mannan oligosaccharide is 4-6:

1.

3. The compound microecological preparation according to claim 1, characterized in that: The weight ratio of the lysine-containing Bacillus to the mannan oligosaccharide is 5:

1.

4. The compound microecological preparation according to any one of claims 1-3, characterized in that: The compound microecological preparation also contains water.

5. The application of any one of the compound microecological preparations according to claims 1-4 in reducing aflatoxin B1 in feed.

6. A feed, characterized in that: The feed contains any one of the compound microecological preparations according to claims 1-4, as well as peanut meal and / or corn and / or soybean meal.

7. The feed according to claim 6, characterized in that: The feed is obtained by fermenting peanut meal and / or corn and / or soybean meal with the above-mentioned compound microecological preparation.

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