Primer set and application thereof, and training method of bacterial vaginosis diagnosis model

By combining multiplex PCR primer sets and high-throughput sequencing with machine learning models, the insufficient sensitivity and specificity of BV detection in existing technologies have been addressed, achieving efficient and low-cost BV diagnosis that is suitable for clinical applications.

CN120866543BActive Publication Date: 2026-06-19PEOPLES HOSPITAL PEKING UNIV +1
View PDF 5 Cites 0 Cited by

Patent Information

Application Number
CN202510773517.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-06-11
Publication Date
2026-06-19
Estimated Expiration
2045-06-11

AI Technical Summary

Technical Problem

Existing technologies lack sufficient sensitivity and specificity in BV detection, failing to fully reflect the vaginal microbiome structure. Furthermore, the high cost and complex operation of these technologies make them difficult to popularize in clinical practice.

Method used

We used multiplex PCR primers for targeted amplification, combined with high-throughput sequencing and machine learning models, to detect 12 target pathogens and construct a diagnostic model for bacterial vaginosis.

Benefits of technology

It achieves high sensitivity and specificity in BV detection, covers more pathogens, simplifies operation, reduces costs, and is suitable for clinical applications.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120866543B_ABST
    Figure CN120866543B_ABST
Patent Text Reader

Abstract

This invention relates to the field of gene detection technology, specifically to a primer set and its application, and a training method for a bacterial vaginosis diagnostic model. The primer set, used for diagnosing bacterial vaginosis, includes specific primers for specifically amplifying target regions of the following pathogens: *Atopobacterium vaginalis*, *Gardnerella vaginalis*, *Cyclophorus 1*, *Cyclophorus 2*, *Bacterial Vaginosis-Associated Bacteria 2*, *Animal Curvularia clarithii*, *Animal Curvularia emblica*, *Prevotella 2*, *Lactobacillus curvularia*, *Lactobacillus indolentus*, *Lactobacillus gasseri*, and *Lactobacillus janniae*. This invention also provides the application of this primer set and a training method for a bacterial vaginosis diagnostic model. Using this primer set, high-throughput sequencing can be performed on test samples, simultaneously detecting 12 target pathogens, and further constructing a bacterial vaginosis diagnostic model, which can be used for the clinical diagnosis of bacterial vaginosis with good specificity and sensitivity.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Primer group, probe group and kit for evaluating vagina health condition and bacterial vaginitis

    CN117344038A

  • Construction method of diagnostic model of bacterial vaginosis

    CN118072818A

  • Primer combination for detecting colpitis-related microorganisms and application thereof

    CN118853922A

  • Composition and kit for detecting pathogens of bacterial vaginitis and application of composition and kit

    CN116121425A

  • Primer and method for detecting pathogens related to bacterial vaginitis and application

    CN119193871A