Pharmaceutical composition for reducing blood pressure as well as pill and application thereof

Abstract

The invention provides a pharmaceutical composition for reducing blood pressure as well as pills and application thereof, and relates to the field of medicines. The pharmaceutical composition for reducing blood pressure comprises the following raw material medicines by weight: 8-10 g of red sandalwood, 7-9 g of hedyotis diffusa, 10-12 g of emblic leafflower fruit, 7-9 g of notopterygium root, 6-8 g of myrobalan, 5-7 g of caragana euonymus, 4-6 g of agilawood, 4-6 g of lignum dalbergiae odoriferae, 5-7 g of lagotis brachystachya, 6-8 g of pomegranate rind and 3-5 g of white mulberry root-bark. The pharmaceutical composition prepared by the invention has the effects of stably reducing blood pressure, soothing the nerves and tranquilizing mind, dredging collaterals and the like, is used for treating hypertension, has a good effect, can help to recover a normal blood pressure level, has no obvious influence on the heart rate, can better realize stable blood pressure reduction, and has good safety.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medicine, in particular to a pharmaceutical composition for reducing blood pressure, a pill thereof and application. BACKGROUND

[0002] Hypertension is one of the most common cardiovascular diseases worldwide, and the prevalence continues to rise. The blood pressure of patients with hypertension is higher than that of normal people, which is a major risk factor for stroke, myocardial infarction and renal failure. At present, clinical treatment mainly relies on five categories of antihypertensive drugs: diuretics, calcium antagonists, angiotensin converting enzyme inhibitors, angiotensin II receptor antagonists and beta blockers. These drugs usually need to be used in combination to enhance efficacy, especially for patients with grade 2 or above hypertension or grade 3 or above hypertension, and the combination of drugs reduces the drug compliance and affects the treatment effect. Therefore, it is urgent to develop a new antihypertensive drug formula to improve the antihypertensive effect while achieving stable blood pressure reduction. SUMMARY

[0003] In view of this, the present application provides a pharmaceutical composition for reducing blood pressure, a pill thereof and application. The pharmaceutical composition of the present application has good antihypertensive effect and can achieve stable blood pressure reduction.

[0004] The technical scheme of the present application is as follows: A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in a weight ratio: Pterocarpus santalinus 8-10 g, Vaccinium bracteatum 7-9 g, Phyllanthus emblica 10-12 g, Notopterygium 7-9 g, Terminalia chebula 6-8 g, Caragana jubata 5-7 g, Aquilaria sinensis 4-6 g, Dalbergia odorifera 4-6 g, Lagotis brevituba 5-7 g, Punica granatum 6-8 g, and Morus alba 3-5 g.

[0005] Further, the pharmaceutical composition for reducing blood pressure comprises the following raw materials in a weight ratio: Pterocarpus santalinus 9 g, Vaccinium bracteatum 8 g, Phyllanthus emblica 11 g, Notopterygium 8 g, Terminalia chebula 7 g, Caragana jubata 6 g, Aquilaria sinensis 5 g, Dalbergia odorifera 5 g, Lagotis brevituba 6 g, Punica granatum 7 g, and Morus alba 4 g.

[0006] Further, the preparation method of the pharmaceutical composition comprises the following specific steps: (1) supercritical CO2 extraction of Pterocarpus santalinus, Aquilaria sinensis and Dalbergia odorifera to obtain a complex extract, and then β-cyclodextrin inclusion to obtain an inclusion compound; (2) Vaccinium bracteatum, Phyllanthus emblica, Notopterygium, Terminalia chebula, Caragana jubata, Lagotis brevituba, Punica granatum and Morus alba are added to 6-8 times of 65%-75% v / v ethanol for reflux extraction, the extract is collected, ethanol is recovered, concentrated, powdered and sieved to obtain a mixture; (3) mixing the inclusion compound and the mixture to obtain the pharmaceutical composition.

[0007] Further technical solutions are that in step (1), the pressure of supercritical CO2 extraction is 15-20 Mpa, the temperature is 40-45 DEG C, and the extraction time is 1-2 h.

[0008] Further technical solutions are that in step (1), the inclusion of the beta-cyclodextrin is specifically performed by adding beta-cyclodextrin into water to form a saturated solution, adding the complex extract under stirring, stirring uniformly, and then performing suction filtration to obtain the inclusion compound, and vacuum drying to obtain the inclusion compound.

[0009] Further technical solutions are that in step (2), the reflux extraction is performed for 2-3 times, and each time is 1-2 h.

[0010] Further technical solutions are that in step (2), the concentration is specifically performed by concentrating at 60-65 DEG C to a relative density of 1.2-1.3 of the extract, and the crushing and sieving use a 80-120 mesh sieve.

[0011] A pill for reducing blood pressure is prepared from the pharmaceutical composition for reducing blood pressure according to any one of the present application.

[0012] Further technical solutions are that the pill for reducing blood pressure further comprises a pharmaceutically acceptable excipient.

[0013] The pharmaceutical composition according to any one of the present application is applied to the preparation of a medicine for reducing blood pressure.

[0014] Compared with the prior art, the present application has the following beneficial effects: (1) The pharmaceutical composition prepared by the present application has good effect for reducing blood pressure, can help restore normal blood pressure level, and has no obvious effect on heart rate, can better achieve stable blood pressure reduction, and has good safety.

[0015] (2) In the pharmaceutical composition of the present application, the sandalwood activates blood circulation and dredges collaterals, the shortspur sanicle clears heat and removes dampness, and the phyllanthus emblica clears heat and cools blood, and cooperates to reduce blood pressure, and is the monarch drug; the eaglewood relieves asthma, the agarwood activates blood circulation and promotes qi, the Chinese juniper activates blood circulation and dredges channels, and the shortspur sanicle reduces blood pressure, and is the ministerial drug, and cooperates to enhance the curative effect of the monarch drug; the mulberry bark relieves lung and asthma, the Terminalia chebula astringes intestines and contracts lung, the Qianghuo removes wind and dampness, dredges collaterals and relieves pain, and the pomegranate peel astringes intestines and stops bleeding, and is the auxiliary drug, and plays the effects of stable blood pressure reduction, tranquilization, and dredging collaterals, and is used for treating hypertension, and has good effect.

[0016] (3) The pharmaceutical composition is treated by the process of the present application, and the blood pressure reduction effect is further improved, and the effects of stable blood pressure reduction are enhanced. DETAILED DESCRIPTION

[0017] In order to better understand the technical content of the present application, the following specific embodiments are provided to further illustrate the present application. The experimental methods used in the embodiments of the present application are all conventional methods unless otherwise specified. The materials, reagents, etc. used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.

[0018] Viola Yedoensis: The product is the whole plant of Viola Yedoensis. Viola yedoensis Makino of the Violaceae.

[0019] Short-bud Lagotis: The product is the whole plant of Short-bud Lagotis Lagotis brachystachya Maxim. of the Violaceae.

[0020] Example 1 A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in a weight ratio: Pterocarpus santalinus 8g, Viola Yedoensis 9g, Phyllanthus emblica 10g, Notopterygium 9g, Terminalia chebula 6g, Caragana jubata 5g, Agilawood 6g, Oud 6g, Short-bud Lagotis 5g, Punica granatum 8g, Morus alba 5g.

[0021] The preparation method of the above-mentioned pharmaceutical composition comprises the following specific steps: (1) The Pterocarpus santalinus, Agilawood and Oud are extracted by supercritical CO2 for 1.5h, and the extraction conditions are pressure 15-20Mpa and temperature 40-45℃, to obtain a complex extract. The complex extract is wrapped with β-cyclodextrin. The β-cyclodextrin is dissolved in water to form a saturated solution, and the complex extract is added under stirring. After stirring, the mixture is filtered, vacuum dried, and the wrapped product is obtained. (2) The Viola Yedoensis, Phyllanthus emblica, Notopterygium, Terminalia chebula, Caragana jubata, Short-bud Lagotis, Punica granatum and Morus alba are added to 7 times 70% v / v ethanol and refluxed for 2 times, each for 1.5h. The extract is collected, the ethanol is recovered, and the extract is concentrated to a relative density of 1.25 at 60℃. The mixture is ground through a 100 mesh sieve to obtain a mixture. (3) The wrapped product and the mixture are mixed to obtain a pharmaceutical composition, which is made into a pill.

[0022] Example 2 A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in a weight ratio: Pterocarpus santalinus 10g, Viola Yedoensis 7g, Phyllanthus emblica 12g, Notopterygium 7g, Terminalia chebula 8g, Caragana jubata 7g, Agilawood 4g, Oud 4g, Short-bud Lagotis 7g, Punica granatum 6g, Morus alba 4g.

[0023] The preparation method of the above-mentioned pharmaceutical composition is consistent with that of Example 1, comprising the following specific steps: (1) supercritical CO2 extraction of red sandalwood, eaglewood, and lycium for 1.5 hours, with extraction conditions of pressure 15-20 Mpa and temperature 40-45℃, to obtain a complex extract, which is then wrapped with β-cyclodextrin, β-cyclodextrin is dissolved in water to form a saturated solution, the complex extract is added under stirring, and stirring is performed until uniform, then filtration is performed, to obtain a wrapping product, which is dried in vacuum to obtain a wrapped product; (2) adding 7 times 70% v / v ethanol to extract the following ingredients by reflux extraction for 2 times, each for 1.5 hours: rabdosia, emblic, nuancao, harada, devil arrow juniper, short-eared rabbit, pomegranate peel, mulberry bark, the extract is collected, ethanol is recovered, and the extract is concentrated to a relative density of 1.25 at 60℃, then it is pulverized through a 100-mesh sieve to obtain a mixture; (3) mixing the wrapped product and the mixture to obtain a pharmaceutical composition, which is made into a pill.

[0024] Example 3 A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in the following proportions by weight: red sandalwood 9g, rabdosia 8g, emblic 11g, nuancao 8g, harada 7g, devil arrow juniper 6g, eaglewood 5g, lycium 5g, short-eared rabbit 6g, pomegranate peel 7g, and mulberry bark 4g.

[0025] The preparation method of the above pharmaceutical composition is consistent with that of Example 1, comprising the following specific steps: (1) supercritical CO2 extraction of red sandalwood, eaglewood, and lycium for 1.5 hours, with extraction conditions of pressure 15-20 Mpa and temperature 40-45℃, to obtain a complex extract, which is then wrapped with β-cyclodextrin, β-cyclodextrin is dissolved in water to form a saturated solution, the complex extract is added under stirring, and stirring is performed until uniform, then filtration is performed, to obtain a wrapping product, which is dried in vacuum to obtain a wrapped product; (2) adding 7 times 70% v / v ethanol to extract the following ingredients by reflux extraction for 2 times, each for 1.5 hours: rabdosia, emblic, nuancao, harada, devil arrow juniper, short-eared rabbit, pomegranate peel, mulberry bark, the extract is collected, ethanol is recovered, and the extract is concentrated to a relative density of 1.25 at 60℃, then it is pulverized through a 100-mesh sieve to obtain a mixture; (3) mixing the wrapped product and the mixture to obtain a pharmaceutical composition, which is made into a pill.

[0026] Example 4 A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in the following proportions by weight: red sandalwood 9g, rabdosia 8g, emblic 11g, nuancao 8g, harada 7g, devil arrow juniper 6g, eaglewood 5g, lycium 5g, short-eared rabbit 6g, pomegranate peel 7g, and mulberry bark 4g. The pharmaceutical composition formula is consistent with that of Example 3.

[0027] The preparation method of the above pharmaceutical composition comprises the following specific steps: (1) supercritical CO2 extraction of Pterocarpus santalinus, Lignum aquilariae resiniferum, Dalbergia odorifera, for 1 h, extraction conditions: pressure 15-20 Mpa, temperature 40-45 °C, to obtain the complex extract, and then β-cyclodextrin is used for inclusion, β-cyclodextrin is dissolved in water to form a saturated solution, and then the complex extract is added under stirring, and stirring is performed until uniform, and then filtration is performed to obtain inclusion compound, and vacuum drying is performed to obtain the inclusion compound; (2) extract Rubus corchorifolius, Phyllanthus emblica, Notopterygium incisum, Terminalia chebula, Caragana jubata, Lagotis brachyota, Punica granatum, Morus alba by adding 6 times of 75% v / v ethanol for reflux extraction for 3 times, 1 h each time, collect the extract, recover the ethanol, and concentrate at 60 °C to a relative density of 1.25 of the extract, crush and pass through a 100 mesh sieve to obtain a mixture; (3) mix the inclusion compound and the mixture to obtain a pharmaceutical composition, and prepare a pill.

[0028] Example 5 A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in a weight ratio: Pterocarpus santalinus 9 g, Rubus corchorifolius 8 g, Phyllanthus emblica 11 g, Notopterygium incisum 8 g, Terminalia chebula 7 g, Caragana jubata 6 g, Lignum aquilariae resiniferum 5 g, Dalbergia odorifera 5 g, Lagotis brachyota 6 g, Punica granatum 7 g, Morus alba 4 g. The pharmaceutical composition formula is consistent with Example 3.

[0029] The preparation method of the above pharmaceutical composition comprises the following specific steps: (1) supercritical CO2 extraction of Pterocarpus santalinus, Lignum aquilariae resiniferum, Dalbergia odorifera, for 2 h, extraction conditions: pressure 15-20 Mpa, temperature 40-45 °C, to obtain the complex extract, and then β-cyclodextrin is used for inclusion, β-cyclodextrin is dissolved in water to form a saturated solution, and then the complex extract is added under stirring, and stirring is performed until uniform, and then filtration is performed to obtain inclusion compound, and vacuum drying is performed to obtain the inclusion compound; (2) extract Rubus corchorifolius, Phyllanthus emblica, Notopterygium incisum, Terminalia chebula, Caragana jubata, Lagotis brachyota, Punica granatum, Morus alba by adding 8 times of 65% v / v ethanol for reflux extraction for 2 times, 2 h each time, collect the extract, recover the ethanol, and concentrate at 65 °C to a relative density of 1.25 of the extract, crush and pass through a 100 mesh sieve to obtain a mixture; (3) mix the inclusion compound and the mixture to obtain a pharmaceutical composition, and prepare a pill.

[0030] Comparative Example 1 A pharmaceutical composition for reducing blood pressure, comprising the following raw materials in a weight ratio: Pterocarpus santalinus 7 g, Rubus corchorifolius 7 g, Phyllanthus emblica 7 g, Notopterygium incisum 7 g, Terminalia chebula 7 g, Caragana jubata 7 g, Lignum aquilariae resiniferum 7 g, Dalbergia odorifera 7 g, Lagotis brachyota 7 g, Punica granatum 7 g, Morus alba 7 g.

[0031] The preparation method of the pharmaceutical composition of this example is consistent with Example 3, comprising the following specific steps: (1) the supercritical CO2 extraction of red sandalwood, eaglewood, and tonkin sappanwood for 1.5 hours, with the extraction conditions of pressure 15-20 Mpa and temperature 40-45℃, to obtain a compound extract, and then the compound extract is wrapped with β-cyclodextrin, the β-cyclodextrin is dissolved in water to form a saturated solution, the compound extract is added to the saturated solution under stirring, and stirring is performed until the compound extract is uniformly mixed, and then the mixture is filtered under suction to obtain a wrapping product, which is dried under vacuum to obtain a wrapping product; (2) the mixture of the following components is prepared: Indian mulberry, emblic, nuancao, chebula, devil's arrow juniper, short-eared rabbit, pomegranate peel, and mulberry bark, and then the mixture is extracted with 7 times of 70% v / v ethanol by reflux extraction for 2 times, each time for 1.5 hours, the extract is collected, the ethanol is recovered, and the extract is concentrated at 60℃ to a relative density of 1.25, and then the extract is pulverized through a 100-mesh sieve to obtain a mixture; (3) the wrapping product and the mixture are mixed to obtain a pharmaceutical composition, and the pharmaceutical composition is prepared into pills.

[0032] Test Example In the present study, male clean spontaneous hypertensive rats (SHR) are selected as the experimental model, the rats are 11 weeks old, the body weight is in the range of 200 g±10 g, and the systolic pressure is ≥140mmHg. The experimental animals are randomly divided into groups, and each group contains 10 rats. The specific grouping and treatment are as follows: (1) treatment groups 1-4: the pills of Example 1-3 and Comparative Example 1 are respectively administered by gavage, and the gavage dose is 2.5 mg / kg; (2) positive group: captopril, the gavage dose is 2.5 mg / kg; (3) blank control group: the same volume of purified water.

[0033] All groups are administered by gavage once a day, and the continuous intervention lasts for 4 weeks. After 4 weeks, the blood pressure level of each group of SHR is detected by the rat tail artery systolic pressure determination method, each rat is repeatedly measured for 3 times, and the average value is taken as the final blood pressure data; at the same time, the heart rate changes of rats in each group before and after administration are recorded to evaluate the potential influence of the drug on the heart rate.

[0034] Table 1 Influence of administration on the blood pressure of rats

[0035] The results show that, compared with the blank group, the blood pressure of rats in treatment groups 1-4 and the positive group all obviously decreases (P<0.05). Moreover, the blood pressure lowering effect of the pills of Example 1-3 is better than that of the positive group. However, the treatment effect of Comparative Example 1 is poor.

[0036] Table 2 Changes in the heart rate of rats before and after administration

[0037] The results show that the heart rate of rats changes little before and after administration (P>0.05), and the pills prepared from Example 1-3 have no obvious influence on the heart rate of rats.

[0038] The above description is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. within the scope of the present application should be included in the protection scope of the present application.

Claims

1. A pharmaceutical composition for antihypertensive effect, characterized by comprising, The raw medicinal materials include the following ingredients in the weight ratio: Radix Pterocarpus 8-10 g, Herba Clerodendri 7-9 g, Fructus Phyllanthi 10-12 g, Rhizoma Notopterygii 7-9 g, Fructus Terminaliae 6-8 g, Herba Potaniniae 5-7 g, Lignum Aquilariae Resinatum 4-6 g, Lignum Dalbergiae Odoriferae 4-6 g, Herba Euphrasiae 5-7 g, Pericarpium Punicae Granati 6-8 g, and Cortex Mori 3-5 g.

2. The pharmaceutical composition for antihypertensive according to claim 1, wherein The raw medicinal materials include the following ingredients in the weight ratio: Radix Pterocarpus 9 g, Herba Clerodendri 8 g, Fructus Phyllanthi 11 g, Rhizoma Notopterygii 8 g, Fructus Terminaliae 7 g, Herba Potaniniae 6 g, Lignum Aquilariae Resinatum 5 g, Lignum Dalbergiae Odoriferae 5 g, Herba Euphrasiae 6 g, Pericarpium Punicae Granati 7 g, and Cortex Mori 4 g.

3. The pharmaceutical composition for antihypertensive use according to claim 1 or 2, wherein The preparation method of the pharmaceutical composition includes the following specific steps: (1) supercritical CO2 extraction of Radix Pterocarpus, Lignum Aquilariae Resinatum, and Lignum Dalbergiae Odoriferae to obtain a compound extract, and then β-cyclodextrin inclusion to obtain an inclusion compound; (2) reflux extraction of Herba Clerodendri, Fructus Phyllanthi, Rhizoma Notopterygii, Fructus Terminaliae, Herba Potaniniae, Herba Euphrasiae, Pericarpium Punicae Granati, and Cortex Mori with 6-8 times of 65%-75% v / v ethanol, collection of the extract, recovery of ethanol, concentration, powdering, and sieving to obtain a mixture; (3) mixing of the inclusion compound and the mixture to obtain the pharmaceutical composition.

4. The pharmaceutical composition for antihypertensive according to claim 3, wherein In step (1), the supercritical CO2 extraction is carried out at a pressure of 15-20 Mpa and a temperature of 40-45℃ for 1-2 h.

5. The pharmaceutical composition for antihypertensive according to claim 3, wherein In step (1), the β-cyclodextrin inclusion is carried out by adding β-cyclodextrin to water to prepare a saturated solution, adding the compound extract under stirring, stirring uniformly, and then suction filtration to obtain the inclusion compound, which is vacuum dried.

6. The pharmaceutical composition for antihypertensive according to claim 3, wherein In step (2), the reflux extraction is carried out for 2-3 times, each for 1-2 h.

7. The pharmaceutical composition for antihypertensive according to claim 3, wherein In step (2), the concentration is carried out at 60-65℃ to obtain the extract with a relative density of 1.2-1.3; and the powdering is carried out using a 80-120 mesh sieve.

8. A pill for lowering blood pressure, characterized by, The pharmaceutical composition for lowering blood pressure is prepared according to any one of claims 1-7.

9. The pill for antihypertensive according to claim 8, wherein The pharmaceutical composition further includes pharmaceutically acceptable adjuvants.

10. The pharmaceutical composition according to any one of claims 1-7 for lowering blood pressure.

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