Application of cle11 small peptide gene in regulating plant salt tolerance

By cloning and overexpressing the millet SiPROCLE11 gene and applying exogenous SiCLE11 peptides, the unknown mechanism of salt tolerance regulation of millet CLE peptides was solved, resulting in a significant improvement in the salt tolerance of millet, enhanced root growth and ion homeostasis, and improved plant resistance to salt stress.

CN120905250BActive Publication Date: 2026-07-10SHANDONG EXPRESSWAY URBAN & RURAL DEV GRP CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG EXPRESSWAY URBAN & RURAL DEV GRP CO LTD
Filing Date
2025-09-18
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

The salt tolerance regulation mechanism of millet CLE peptides has not yet been explored, and existing technologies are insufficient to effectively improve the salt tolerance of millet and other plants.

Method used

The SiPROCLE11 gene was cloned, and SiPROCLE11 overexpression and silencing materials were constructed. The artificially synthesized small peptide SiCLE11p was exogenously applied to promote the root growth of millet, reduce Na+ accumulation, and enhance the salt tolerance of millet.

Benefits of technology

Overexpression of the SiPROCLE11 gene and application of the small peptide SiCLE11p significantly improved the salt tolerance of millet, enhanced root growth, reduced Na+ accumulation, increased chlorophyll content, maintained ion homeostasis, and enhanced resistance to salt stress.

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Abstract

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.
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