Non-targeted all-component screening and quantifying method for organic matter

Abstract

The invention discloses an organic matter non-targeted all-component screening and quantifying method, and belongs to the technical field of chemical analysis. The technical problem to be solved is to provide a non-targeted all-component screening and quantification method for organic matters, which can qualitatively detect thermally generated organic matters, especially components of human-derived organic matters as comprehensively as possible. According to the technical scheme, the method is characterized in that a solid-phase extraction method is adopted for extracting, purifying and concentrating the thermally-formed organic matter, and non-targeted all-component screening and semi-quantitative analysis are conducted on the thermally-formed organic matter in combination with a comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry technology.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of chemical analysis, and in particular to a non-targeted full-component screening and quantitative method for organic matter. BACKGROUND

[0002] For understanding the technical content of the present application:

[0003] Pyrolytic organic matter refers to a difficult-to-degrade carbon-rich mixture generated by incomplete combustion of biomass or fossil fuels, which is an important component of dissolved organic matter, and its content accounts for about 10% of total organic matter in freshwater systems, affecting the global biogeochemical cycle of carbon in the ecological system and the environment. Pyrolytic organic matter is an important component of forest fire emissions, which can be transported and deposited by the atmosphere into the soil, atmosphere and water environment. Pyrolytic organic matter has abundant oxygen-containing functional groups such as carboxyl and hydroxyl groups, and has a condensed polycyclic structure, which mediates the environmental transformation process of environmental pollutants, and can significantly affect the migration, transformation and environmental risk of environmental pollutants. Therefore, through non-targeted screening and relative quantitative research on the composition of pyrolytic organic carbon from biomass smoke sources, reference can be provided for further exploring its environmental processes and ecological effects in the environment. At present, the research on the composition and structure of pyrolytic organic matter mainly focuses on the analysis of fluorescent groups and functional groups, and the qualitative analysis of non-targeted full components is relatively less, and the quantitative data of organic compounds in pyrolytic organic matter are not clear.

[0004] At present, gas chromatography-mass spectrometry (GC-MS) is a commonly used analysis method for determining the composition of organic compounds. However, due to the complex composition of pyrolytic organic matter, traditional GC-MS mainly uses one-dimensional chromatographic columns of different polarities to separate organic compounds. Due to the limitation of chromatographic column materials, some organic compounds are difficult to separate in one-dimensional chromatographic columns and are easily co-eluted. Therefore, by connecting two chromatographic columns of different polarities, GCxGC-TOF-MS improves the detection sensitivity and can separate compounds with similar properties by connecting a specific modulator to enrich the sample. TOF-MS can scan the large capacity peak information of two-dimensional column flow at high frequency to realize the complete collection of organic matter information. At present, this technology has been widely used in the detection and analysis of different volatile organic compounds, for example, GCxGC-TOF-MS technology is used to screen trace organic pollutants in different samples at shale gas sites. Due to the low content of organic matter in the ecological environment system, it is not possible to directly use the existing GCxGC-TOF-MS technology to detect pyrolytic organic matter, therefore, the development of a rapid and efficient pre-concentration method combined with GCxGC-TOF-MS technology is the key direction for further exploring the non-targeted full-component screening and relative quantification of pyrolytic organic matter.

[0005] The retrieved relevant patent documents are:

[0006] The document discloses a method for identifying crude oil types based on full two-dimensional gas chromatography-time-of-flight mass spectrometry, which comprises the following steps: dissolving a to-be-identified crude oil sample with a diluent, then adding 5α-androsta- n standard into the system to obtain a crude oil sample; performing chromatographic analysis on the crude oil sample by using full two-dimensional gas chromatography-time-of-flight mass spectrometry, and taking the ratio of the sum of the weights of n-alkanes, isomeric alkanes, monocyclic alkanes, bicyclic alkanes, tricyclic alkanes, tetracyclic alkanes and pentacyclic alkanes in the crude oil sample to the weight of the to-be-identified crude oil sample as S; taking the ratio of the sum of the weights of monocyclic alkanes, bicyclic alkanes, tricyclic alkanes, tetracyclic alkanes and pentacyclic alkanes in the crude oil sample to the weight of the to-be-identified crude oil sample as N; and identifying the type of the crude oil sample according to the ratio of S to N.

[0007] The retrieved relevant non-patent documents are:

[0008] The document discloses a method for identifying crude oil types based on full two-dimensional gas chromatography-time-of-flight mass spectrometry, which comprises the following steps: dissolving a to-be-identified crude oil sample with a diluent, then adding 5α-androsta- n standard into the system to obtain a crude oil sample; performing chromatographic analysis on the crude oil sample by using full two-dimensional gas chromatography-time-of-flight mass spectrometry, and taking the ratio of the sum of the weights of n-alkanes, isomeric alkanes, monocyclic alkanes, bicyclic alkanes, tricyclic alkanes, tetracyclic alkanes and pentacyclic alkanes in the crude oil sample to the weight of the to-be-identified crude oil sample as S; taking the ratio of the sum of the weights of monocyclic alkanes, bicyclic alkanes, tricyclic alkanes, tetracyclic alkanes and pentacyclic alkanes in the crude oil sample to the weight of the to-be-identified crude oil sample as N; and identifying the type of the crude oil sample according to the ratio of S to N.

[0009] The existing technology represented by the foregoing documents at least has the following unsolved technical problems or defects:

[0010] The existing technology has not applied the test method combining full two-dimensional gas chromatography-time-of-flight mass spectrometry to the detection of components of thermal organic matter.

[0011] In solving the above problems or overcoming the above defects, the present application has encountered the following difficulties and obstacles:

[0012] Thermal organic matter needs to be highly extracted and purified before being tested by comprehensive two-dimensional gas chromatography-time of flight mass spectrometry. SUMMARY

[0013] The present application aims to provide:

[0014] An organic matter non-targeted full component screening and quantification method and related technologies are provided to solve the technical problems of providing an organic matter non-targeted full component screening and quantification method, which can qualitatively detect the components of thermal organic matter as comprehensively as possible, and combinations thereof.

[0015] Explanation of terms:

[0016] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the subject matter of the claims belongs. All publications, patent applications, patents, and other references cited herein are incorporated by reference in their entirety unless otherwise indicated. If there is a plurality of definitions for a term herein, those in this section prevail.

[0017] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the subject matter claimed. In this application, the use of the singular includes the plural unless specifically stated otherwise. It should also be noted that, as used in this application, the term "or" as used herein, unless otherwise indicated, means "and / or." Furthermore, the use of the term "including" as well as other forms such as "include", "includes," and "included" is not limiting.

[0018] The definitions of standard chemical terms can be found in the reference "Analytical Chemistry, edited by Wuhan University, Higher Education Press, fourth edition, 2004".

[0019] Unless otherwise specified, conventional methods within the scope of the art are used, such as crushing, elution, activation, concentration, etc.

[0020] Unless a specific definition is provided, the use of each type of commercially available product used herein is in accordance with standard techniques. For example, the use of reagent kits can be carried out according to the manufacturer's instructions, or in accordance with ways known in the art or the instructions of the present application. In general, the above-mentioned techniques and methods can be carried out according to conventional methods well known in the art, according to the descriptions in the multiple summary and more specific literatures cited and discussed in the present specification.

[0021] As used herein, the term "chromatography" refers to a physical separation method in which the components (i.e., chemical constituents) to be separated are distributed between two phases, one of which is stationary (the stationary phase) and the other of which (the mobile phase) moves in a definite direction. The mobile phase can be a gas ("gas chromatography", "GC") or a liquid ("liquid chromatography", "LC"). Chromatographic output data can be used in embodiments of the methods described herein.

[0022] As used herein, the term "scan" refers to a mass spectrum associated with a particular separation index. For example, a system using chromatographic separation techniques can generate multiple scans, each at a different retention time.

[0023] As used herein, the term "run time" refers to the time from sample injection to the production of instrument data. Total run time includes chromatography and mass spectrometry of the sample.

[0024] In a first aspect, the present application provides: a method for non-targeted full component screening and quantification of organic matter.

[0025] In which, the technical features include: biomass, pyrolysis, filter membrane, elution, solid phase extraction, two-dimensional gas chromatography-time of flight mass spectrometry, etc.

[0026] In which, the technical feature biomass is selected from: wood, branches, sawdust, sawdust, board skin, fruit shell, fruit core, corn straw, straw, bean straw, cattail.

[0027] In which, the technical feature biomass is preferably: branches, sawdust, sawdust, board skin, fruit core, corn straw, straw, cattail.

[0028] In which, the technical feature biomass is further preferably: branches, sawdust, sawdust, corn straw, straw, cattail.

[0029] In which, the technical feature biomass is more preferably: cattail.

[0030] In which, the technical feature pyrolysis is selected from: pyrolysis temperature is 450-550℃, atmosphere is inert atmosphere.

[0031] In which, the technical feature pyrolysis is preferably: pyrolysis temperature is 450-500℃, atmosphere is nitrogen or argon.

[0032] In which, the technical feature pyrolysis is further preferably: pyrolysis temperature is 500℃, atmosphere is nitrogen.

[0033] In which, the technical feature filter membrane is selected from: the pore size of the filter membrane is 0.4-0.5μm.

[0034] The technical feature filter membrane is preferably 0.4 μm, 0.41 μm, 0.42 μm, 0.43 μm, 0.44 μm, 0.45 μm, 0.46 μm, 0.47 μm, 0.48 μm, 0.49 μm, 0.50 μm.

[0035] The technical feature filter membrane is further preferably 0.4 μm, 0.41 μm, 0.42 μm, 0.43 μm, 0.44 μm, 0.45 μm, 0.46 μm, 0.47 μm, 0.48 μm.

[0036] The technical feature filter membrane is further preferably 0.4 μm, 0.41 μm, 0.42 μm, 0.43 μm, 0.44 μm, 0.45 μm, 0.46 μm, 0.47 μm, 0.48 μm.

[0037] The technical feature elution is selected from the following: the eluent is water, and the elution process is accompanied by ultrasonic.

[0038] The technical feature elution is preferably: the eluent is purified water, and the ultrasonic time is 50-70 min.

[0039] The technical feature elution is further preferably: the eluent is selected from pure water, deionized water, pure water, and distilled water; and the ultrasonic time is 50-60 min.

[0040] The technical feature elution is further preferably: the eluent is deionized water, and the ultrasonic time is 60 min.

[0041] The technical feature solid phase extraction is selected from the following: the chromatographic column is Agilent Bond Elut PPL; the chromatographic column needs to be activated and impurities removed before elution; and the eluent is an alcohol solvent.

[0042] The technical feature solid phase extraction is preferably:

[0043] The chromatographic column is Agilent Bond Elut PPL, 500 mg, 6 mL, USA;

[0044] The reagent for removing impurities is a hydrochloric acid solution with a pH of 2;

[0045] The reagent for drying is nitrogen with a concentration of 99.99%;

[0046] The eluent is methanol.

[0047] The technical feature two-dimensional gas chromatography-time-of-flight mass spectrometry is selected from the following:

[0048] (1) Two-dimensional gas chromatography:

[0049] The one-dimensional chromatographic column is DB-5MS 60 m x 0.25 mm x 0.25 μm;

[0050] Two-dimensional chromatographic column is DB-17MS 0.85m*0.25mm*0.15um;

[0051] The modulation column is: SV;

[0052] The column oven: the initial temperature is 45-55 DEG C, and is kept for 3-8 min; the temperature is raised to 280-320 DEG C at the speed of 1-2 DEG C / min, and is kept for 8-12 min;

[0053] Carrier gas: He (99.999%);

[0054] Flow rate: 1.0-1.1 mL / min;

[0055] Injection port: 280-320 DEG C;

[0056] Injection volume: 0.8-1.5 uL;

[0057] Split ratio: no split;

[0058] (2) Time-of-flight mass spectrometry conditions:

[0059] Inlet temperature: -5~5 DEG C (relative to the GC column oven temperature);

[0060] Outlet temperature: 28~32 DEG C (relative to the GC column oven temperature);

[0061] Cold zone temperature: -55~-51 DEG C;

[0062] Modulation period: 3-5 s.

[0063] Ion source temperature: 180-220 DEG C;

[0064] Mass spectrometry transmission line temperature: 260-300 DEG C;

[0065] Ionization voltage: -80~-70 eV;

[0066] Mass range: 50-1200 amu;

[0067] Scan mode: full scan

[0068] Scan rate: 4-6 maps / s;

[0069] Among them, the technical features of two-dimensional gas chromatography-time-of-flight mass spectrometry are preferably:

[0070] (1) Two-dimensional gas chromatography:

[0071] One-dimensional chromatographic column is DB-5MS 60m*0.25mm*0.25um;

[0072] Two-dimensional chromatographic column: DB-17MS 0.85m x 0.25mm x 0.15μm;

[0073] Modulation column: SV;

[0074] Column oven: initial temperature 50℃, hold for 5min, increase to 300℃ at a rate of 2℃ / min, hold for 10min;

[0075] Carrier gas: He (99.999%);

[0076] Flow rate: 1.1mL / min;

[0077] Injection port: 300℃;

[0078] Injection volume: 1.0uL;

[0079] Split ratio: no split;

[0080] (2) Time-of-flight mass spectrometry conditions:

[0081] Inlet temperature: 0℃ (relative to the temperature of GC column oven);

[0082] Outlet temperature: 30℃ (relative to the temperature of GC column oven);

[0083] Cold zone temperature: -51℃;

[0084] Modulation period: 5s;

[0085] Ion source temperature: 200℃;

[0086] Mass spectrometry transmission line temperature: 280℃;

[0087] Ionization voltage: -70eV;

[0088] Mass range: 50-1200amu;

[0089] Scan mode: full scan;

[0090] Scan rate: 4-6 spectra / s.

[0091] Based on further solving or simultaneously solving multiple technical problems of the technical problem of the present application, in the technical solution provided by the first aspect of the present application, the preferred solution includes:

[0092] The first preferred solution: in step S1, the pyrolysis temperature is 450-550℃, the time is 1.5-2.5h, and it is carried out in an inert gas atmosphere. This technical solution further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0093] The second priority scheme: in step S1, the pore size of the filter membrane is 0.4-0.5 μm, and the filter membrane is a glass fiber filter membrane. This technical scheme further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0094] The third priority scheme: in step S1, the elution solvent is water. This technical scheme further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0095] The fourth priority scheme: in step S2, the chromatographic column for solid phase extraction is Agilent Bond ElutPPL. This technical scheme further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0096] The fifth priority scheme: in step S2, the chromatographic column needs to be activated and impurities removed before use. This technical scheme further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0097] The sixth priority scheme: in step S2, the eluent for solid phase extraction is an alcohol solvent. This technical scheme further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0098] The seventh priority scheme: in step S2, the alcohol solvent is methanol. This technical scheme further solves the technical problem of "more comprehensive detection of components in organic matter" on the basis of solving the technical problem of "detection of components in organic matter".

[0099] The eighth priority scheme: in step S3, in the full two-dimensional gas chromatography-time-of-flight mass spectrometry technology,

[0100] (1) Two-dimensional gas chromatography:

[0101] The one-dimensional chromatographic column is DB-5MS 60m x 0.25mm x 0.25μm;

[0102] The two-dimensional chromatographic column is DB-17MS 0.85m x 0.25mm x 0.15μm;

[0103] The modulation column is: SV;

[0104] The column oven: the initial temperature is 50℃, and is kept for 5min; the temperature is raised to 300℃ at a rate of 2℃ / min, and is kept for 10min;

[0105] Carrier gas: He (99.999%);

[0106] Flow rate: 1.1 mL / min;

[0107] Injection port: 300℃;

[0108] Injection volume: 1.0 uL;

[0109] Split ratio: none;

[0110] (2) Time-of-flight mass spectrometry conditions:

[0111] Inlet temperature: 0℃ (relative to GC column oven temperature);

[0112] Outlet temperature: 30℃ (relative to GC column oven temperature);

[0113] Cold zone temperature: -51℃;

[0114] Modulation period: 5s;

[0115] Ion source temperature: 200℃;

[0116] Mass spectrometry transmission line temperature: 280℃;

[0117] Ionization voltage: -70eV;

[0118] Mass range: 50-1200amu;

[0119] Scan mode: full scan;

[0120] Scan rate: 4-6 spectra / s.

[0121] The technical solution solves the technical problem of detecting components in organic matter, and further solves the technical problem of more comprehensive detection of components in organic matter.

[0122] The present application has at least the following beneficial effects:

[0123] Compared with the prior art, the present application has better technical effects in detecting the number of components in thermally generated organic matter. BRIEF DESCRIPTION OF DRAWINGS

[0124] Figure 1 The present application has at least the following beneficial effects:

[0125] Figure 2 The present application has at least the following beneficial effects: DETAILED DESCRIPTION

[0126] The following non-limiting examples can provide a more complete understanding of the application to those of ordinary skill in the art, but are not intended in any way to limit the scope of the application. The following merely illustrates the scope of the application claimed, and those skilled in the art will recognize various modifications and changes that are encompassed within the scope of the application as set forth in the claims.

[0127] The application is further described in the following specific examples. The various instruments, devices, equipment, reagents, products, etc. used in the examples of the application are obtained through conventional commercial channels, unless otherwise stated.

[0128] Part of the instrument and reagent description:

[0129] 1. Instruments:

[0130] Agilent 8890 GC gas chromatography system with split / splitless injection port;

[0131] SSM1810 snow scene technology solid-state thermal modulator with SV series modulation column (C7-C40);

[0132] Agilent 7250 ATOF high-resolution time-of-flight GC-MS instrument, HES source;

[0133] Tubular atmosphere furnace;

[0134] Solid phase extraction device;

[0135] PPL solid phase extraction column (Agilent Bond Elut PPL, 500 mg, 6 mL, USA);

[0136] Glass fiber filter membrane.

[0137] Software: Canvas 2.0 two-dimensional gas chromatography data processing software (snow scene technology); NIST17 mass spectrum library.

[0138] 2. Reagents:

[0139] Methanol, chromatographically pure;

[0140] Hydrochloric acid, analytical pure;

[0141] Potassium hydroxide, analytical pure;

[0142] Deionized water.

[0143] Example 1

[0144] S1: Take a certain amount of dry and crushed biomass of Typha angustifolia, pass through a 100-mesh steel sieve, and then weigh 20 g of the biomass and place it in a crucible. Heat the biomass to 500℃ under anaerobic (nitrogen, 99.99%) conditions, collect the biomass smoke source thermal organic matter using a 0.45-μm glass fiber filter membrane, extract the biomass smoke source thermal organic matter on the glass fiber filter membrane with pure water, and then perform solid-phase extraction on the extracted organic matter to obtain a methanol solution of the biomass smoke source thermal organic matter.

[0145] S2: Perform full-component non-targeted determination of the obtained methanol solution of the biomass smoke source thermal organic matter using two-dimensional gas chromatography-time-of-flight mass spectrometry.

[0146] The two-dimensional gas chromatography-time-of-flight mass spectrometry test conditions are as follows:

[0147] (1) Chromatographic conditions:

[0148] Chromatographic column:

[0149] One-dimensional column: DB-5MS, 60 m x 0.25 mm x 0.25 μm;

[0150] Two-dimensional column: DB-17MS, 0.85 m x 0.25 mm x 0.15 μm;

[0151] Modulation column: SV.

[0152] Column oven: initial temperature of 50℃, holding for 5 min; temperature rising to 300℃ at a rate of 2℃ / min, holding for 10 min;

[0153] Carrier gas: He (99.999%); flow rate: 1.1 mL / min; injection port: 300℃; injection volume: 1.0 uL; split ratio: no split.

[0154] (2) Time-of-flight mass spectrometry conditions:

[0155] Inlet temperature: 0℃ (relative to the GC column oven temperature); outlet temperature: 30℃ (relative to the GC column oven temperature); cold zone temperature: -51℃; modulation period: 5 s.

[0156] Ion source temperature: 200℃;

[0157] Mass spectrometry transmission line temperature: 280℃;

[0158] Ionization voltage: -70 eV;

[0159] Mass range: 50-1200 amu;

[0160] Scan mode: full scan;

[0161] Scan rate: 5 spectra / s.

[0162] The two-dimensional gas chromatography-time of flight mass spectrometry data analysis: the Canvas data processing software is used for automatic peak detection and merging, all peaks with a signal-to-noise ratio greater than 10 are set as detection thresholds, after removing invalid peaks such as column loss, matching is carried out according to the NIST17 spectrum library.

[0163] The detection method uses a forward column configuration: the one-dimensional column is a weak polar column, and the two-dimensional column is a medium polar column; therefore, the stronger the polarity of the components in the sample, the longer the retention time on the two-dimensional column. According to the distribution rule (tile effect of homologous compounds) of the full two-dimensional spectrum of the sample and the mass spectrum characteristic ions, the distribution of different groups of compounds in the spectrum can be obtained Figure 1 and Figure 2 ).

[0164] The full two-dimensional chromatogram profile (total ion flow chromatogram: TIC graph) of the sample is shown in Figure 1 , the horizontal axis is the one-dimensional retention time, the vertical axis is the two-dimensional retention time, the total ion flow chromatogram corresponding signal intensity is represented by color, and blue to red represents the relative signal from weak to strong; the full two-dimensional chromatogram 3D view of the sample is shown in Figure 2 , the horizontal axis D1 is the one-dimensional retention time (min), and the vertical axis D2 is the two-dimensional retention time (s). It can be seen from Figure 1 and Figure 2 that the one-dimensional gas chromatography separation of organic compounds has a part of co-flow phenomenon, the use of GCxGC two-dimensional orthogonal separation can greatly improve the capacity of the chromatographic peak, realize the high-throughput detection of unknown organic compounds, and has good separation effect.

[0165] Through the matching results of RI correction and NIST17 library using the nC7-nC35 normal alkane series standard sample, the qualitative results of different groups of compounds are obtained, the mass spectrum information of each compound is automatically matched with the NIST17 spectrum library, and the compound with the highest similarity is listed in the peak list. Since many compounds in the sample have isomers or homologues, manual inspection is required for the qualitative identification of the compound, and after verification by comparing the two-dimensional chromatography peak rules of the homologues, the preliminary identification results are obtained.

[0166] 274 components are detected in the smoke source thermogenic organic matter by using the present application, and the specific results of the relative contents are shown in Table 1-1, Table 1-2, Table 1-3, Table 1-4, Table 1-5, Table 1-6, Table 1-7, Table 1-8, Table 1-9 and Table 1-10.

[0167] Table 1-1. Compound list of smoke source thermogenic organic matter

[0168]

[0169] Table 1-2. Compound list of smoke source thermogenic organic matter

[0170]

[0171] Table 1-3. List of thermal organic compounds from smoke sources

[0172]

[0173] Table 1-4. List of thermal organic compounds from smoke sources

[0174]

[0175] Table 1-5. List of thermal organic compounds from smoke sources

[0176]

[0177] Table 1-6. List of thermal organic compounds from smoke sources

[0178]

[0179] Table 1-7. List of thermal organic compounds from smoke sources

[0180]

[0181] Table 1-8. List of thermal organic compounds from smoke sources

[0182]

[0183] Table 1-9. List of thermal organic compounds from smoke sources

[0184]

[0185] Table 1-10. List of thermal organic compounds from smoke sources

[0186]

[0187] Finally, it should be noted that the above is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A method for non-targeted whole-organism constituent screening and quantification of organic matter, characterized in that, Comprising the following steps: S1: biomass pyrolysis, filter membrane collection, elution and extraction, to obtain biomass smoke source thermal organic matter; S2: the biomass smoke source thermal organic matter obtained in step S1 is concentrated and purified by solid phase extraction method to obtain a sample to be tested; S3: the sample to be tested is subjected to non-targeted full component screening and relative quantitative analysis by full two-dimensional gas chromatography-time of flight mass spectrometry; (1) Two-dimensional gas chromatography: One-dimensional chromatographic column: DB-5MS 60m x 0.25mm x 0.25μm; Two-dimensional chromatographic column: DB-17MS 0.85m x 0.25mm x 0.15μm; Modulation column: SV; Column oven: initial temperature 45-55℃, hold for 3-8min; temperature rising at a rate of 1-2℃ / min to 280-320℃, hold for 8-12min; Carrier gas: He; Flow rate: 1.0-1.1mL / min; Injection port: 280-320℃; Injection volume: 0.8-1.5uL; Split ratio: no split; (2) Time of flight mass spectrometry conditions: Inlet temperature: -5-5℃; Outlet temperature: 28-32℃; Cold zone temperature: -55--51℃; Modulation period: 3-5s; Ion source temperature: 180-220℃; Mass spectrometry transmission line temperature: 260-300℃; Ionization voltage: -80--70eV; Mass range: 50-1200amu; Scan mode: full scan; Scan rate: 4-6spectrum / s.

2. The method of non-targeted whole-organism constituent screening and quantification of organic matter according to claim 1, wherein, In step S1, the pyrolysis temperature is 450-550℃, and the time is 1.5-2.5h, under inert gas atmosphere.

3. The method of claim 1, wherein the organic matter is non-targeted and quantified. 3 In step S1, the pore size of the filter membrane is 0.4-0.5μm, and the filter membrane is a glass fiber filter membrane.

4. The method of non-targeted whole-organism constituent screening and quantification of organic matter of claim 1, wherein, In step S1, the elution solvent is water.

5. The method of claim 1, wherein the organic matter is non-targeted whole component screening and quantification. In step S2, the chromatographic column used in the solid phase extraction method is Agilent Bond Elut PPL.

6. The method of non-targeted whole-organism constituent screening and quantification of organic matter according to claim 5, wherein, In step S2, the chromatographic column needs to be activated and impurities removed before use.

7. The method of non-targeted whole-organism constituent screening and quantification of organic matter of claim 5, wherein, In step S2, the eluent for solid phase extraction is an alcohol solvent.

8. The method of non-targeted whole-organism composition screening and quantification of organic matter according to claim 7, wherein, In step S2, the alcohol solvent is methanol.

9. The method for non-targeted full-component screening and quantification of organic matter according to claim 1, characterized in that, In step S3, in the full two-dimensional gas chromatography-time of flight mass spectrometry, (1) Two-dimensional gas chromatography: One-dimensional chromatographic column: DB-5MS 60m x 0.25mm x 0.25μm; Two-dimensional chromatographic column: DB-17MS 0.85m x 0.25mm x 0.15μm; Modulation column: SV; Column oven: initial temperature 50℃, hold for 5min; temperature rising at a rate of 2℃ / min to 300℃, hold for 10min; Carrier gas: He; Flow rate: 1.1mL / min; Injection port: 300℃; Injection volume: 1.0uL; Split ratio: no split; (2) Time of flight mass spectrometry conditions: Inlet temperature: 0℃; Outlet temperature: 30℃; Cold zone temperature: -51℃; Modulation period: 5s; Ion source temperature: 200℃; Mass spectrometry transmission line temperature: 280℃; Ionization voltage: -70eV; Mass range: 50-1200amu; Scan mode: full scan; Scan rate: 4-6spectrum / s.

10. Use of the method of non-targeted whole composition screening and quantification of organic matter according to any one of claims 1 to 9 for the detection of thermogenic organic matter compositions.

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