Acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide acaricidal, and discloses an acaricidal composition and application thereof.The acaricidal composition comprises an active ingredient A and an active ingredient B. The active ingredient A is a compound shown in the formula I. The structure of the compound shown in the formula I is shown in the specification, and the active ingredient B is pyridaben or fenpyroximate. The mass ratio of the active component A to the active component B is (1: 60)-(48: 1). The acaricidal composition disclosed by the invention has an obvious synergistic effect on the prevention effect of multiple phytophagous pest mites, the use amount of pesticides is effectively reduced, and the pollution to the environment is reduced.

Description

[0001] This invention application is a divisional application of application number 202410842107.4, filed on June 27, 2024, entitled "An acaricide composition and its application". Technical Field

[0002] This invention belongs to the field of pesticide acaricide technology, and discloses an acaricide composition and its application. Background Technology

[0003] Fenpyroximate, also known as 4-[[(E)-(1,3-dimethyl-5-phenoxypyrazole-4-yl)methyleneamino]oxymethyl]tert-butyl benzoate, CAS Registry Number: 134098-61-6, is a pyrazole acaricide with good rapid-acting and long-lasting effects, effective against various spider mites and rust mites. Its structural formula is shown below:

[0004]

[0005] Pyridaben, common English name: pyridaben, chemical name: 2-tert-butyl-5-[(4-tert-butylphenyl)methylthioalkyl]-4-chloropyridazin-3-one, CAS Registry Number: 96489-71-3. Pyridaben is a broad-spectrum, fast-acting contact pyridazinone acaricide that can be used to control various plant-eating mites and is effective throughout the entire life cycle of mites. Its structural formula is shown below:

[0006]

[0007] Compound I is a novel compound independently developed by our company. It has a broad insecticidal spectrum and significant insecticidal and acaricidal effects. The structure of compound I is shown below:

[0008]

[0009] Spider mites, commonly known as red spider mites, belong to the order Tetranychidae of the subclass Acari of the class Arachnidae. They are a very important global agricultural pest that can damage fruit trees, vegetables, and flowers. They often gather on the undersides of host plant leaves, sucking sap and reducing photosynthesis, causing damage such as yellowing and wilting of leaves. In severe cases, they can cause crop death, resulting in significant economic losses to agricultural production. Gall mites are the smallest group of mites and are also an important pest affecting agriculture and forestry. They parasitize the tender leaves, branches, flowers, and fruits of plants, causing abnormal plant tissue formation. They pose a significant threat to fruit trees, affecting their health, productivity, and fruit quality, and severely impacting the economic value of fruit trees.

[0010] Integrated pest management (IPM) is commonly used to control field mite populations, with chemical control being the most important and frequently employed method. To clarify the control efficacy of compound I combined with abamectin or pyridaben against mites, we conducted indoor and field studies on different pesticide combinations and ratios targeting spider mites, citrus pterostilbene, and citrus rust mite. The aim is to provide a scientific basis for the selection and application of chemical pesticides for the control of field mites. Summary of the Invention

[0011] To address the shortcomings of existing technologies, this invention provides an acaricidal composition and its application. The acaricidal composition of this invention has excellent control effects on phytophagous mites, especially against mites such as the carmine spider mite, the two-spotted spider mite, the citrus pterostilbene mite, and the citrus rust mite, effectively reducing the amount of pesticides used and mitigating environmental pollution.

[0012] To achieve the above objectives, the present invention adopts the following technical solution: an acaricidal composition, wherein the active ingredients in the acaricidal composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I, and the structure of the compound of formula I is shown below: The active ingredient B is pyridaben or azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:60 to 48:1, or any value within the above range.

[0013] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:60 to 40:1, or any value within the above range;

[0014] Furthermore, the active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:32 to 40:1, or any value within the above range.

[0015] The active ingredient B is azoxystrobin, and the mass ratio of the compound of formula I to azoxystrobin is 1:60 to 38:1, or any value within the above range.

[0016] Furthermore, the active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:30 to 25:1, or any value within the above range.

[0017] The active ingredient B is azoxystrobin, and the mass ratio of the compound of formula I to azoxystrobin is 1:42 to 38:1, or any value within the above range.

[0018] Furthermore, the active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:25 to 25:1, or any value within the above range.

[0019] The active ingredient B is azoxystrobin, and the mass ratio of the compound of formula I to azoxystrobin is 1:36 to 38:1, or any value within the above range.

[0020] Furthermore, the total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the acaricide composition.

[0021] Furthermore, in addition to the active ingredient, the composition also contains pesticide-permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0022] The wetting agent is selected from one or more of the following: alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series, and wetting and penetrating agent F; and / or

[0023] The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0024] The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0025] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0026] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0027] Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0028] Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0029] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0030] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0031] The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0032] Synergists are selected from synergistic phosphorus, synergistic ether; and / or

[0033] The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0034] Furthermore, the acaricide composition is prepared into a pesticide-permitted formulation, wherein the formulation is a solid or liquid formulation;

[0035] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0036] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0037] Furthermore, the solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension, microemulsion, emulsifiable concentrate, water emulsion, or dispersible oil suspension.

[0038] The present invention also discloses the application of the acaricidal composition described above for the control of phytophagous mites.

[0039] Furthermore, the phytophagous mites mentioned are mites belonging to the Tetranychidae and Erythrophagidae families.

[0040] The beneficial effects of this invention are as follows:

[0041] 1) The acaricide composition of the present invention rationally combines compounds with different mechanisms of action, and has a significant synergistic effect on phytophagous mites;

[0042] 2) The acaricide composition of the present invention effectively reduces the dosage of pesticides, reduces pesticide pollution to the environment, and reduces pesticide residues in crops. Detailed Implementation

[0043] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0044] Formulation preparation example:

[0045] Preparation Example 1: 17% Compound I of Formula 1·pyridaben suspension (1:16)

[0046] Formula composition: 1% Formula I compound, 16% pyridaben, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% alkylaryl polyoxyethylene ether polyoxypropylene ether, 3% tristyrylphenol ethoxylated phosphate, 1% sodium polycarboxylate, 2% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium benzoate, 5% glycerol, 0.5% silicone oil, deionized water to make up the balance;

[0047] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0048] Preparation Example 2: 27% Compound I of Formula 1·pyridaben emulsifiable concentrate (1:8)

[0049] Formula composition: 3% Formula I compound, 24% pyridaben, 18% propylene glycol methyl ether, 15% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 12% DMF, and methyl oleate to make up the balance;

[0050] Preparation method: According to the formula ratio, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable oil required by this invention.

[0051] Preparation Example 3: 15% Compound I·pyridaben wettable powder (1:2)

[0052] Formula composition: 5% Formula I compound, 10% pyridaben, 4% sodium lignosulfonate, 5% naphthalenesulfonate formaldehyde condensate, 2% BX splitting powder, 6% silica, and kaolin to make up the balance;

[0053] Preparation method: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a stirring tank. The mixture is then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0054] Preparation Example 4: 10% Compound I·pyridaben microemulsion (4:1)

[0055] Formula composition: 8% Formula I compound, 2% pyridaben, 12% glyceryl fatty acid ester polyoxyethylene ether, 1% polyoxyethylene dehydrated sorbitan monooleate, 4% styrene-phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 2% sodium fatty alcohol polyoxyethylene ether sulfate, 20% cyclohexanone, 0.5% silicone defoamer, deionized water to make up the balance;

[0056] Preparation method: According to the formula ratio, the active ingredients are completely dissolved in the solvent, and an emulsifier is added to form an oil phase. Dispersant, deionized water and other ingredients are added and stirred evenly to form an aqueous phase. The oil phase is added to the aqueous phase and stirred evenly. The particles are sheared at high speed until the particle size is qualified. After adding the defoamer, the mixture is stirred evenly to obtain the microemulsion product.

[0057] Preparation Example 5: 18% Compound I of Formula 1·pyridaben water-dispersible granules (8:1)

[0058] Formula composition: 16% Formula I compound, 2% pyridaben, 3% styrene phenol polyoxyethylene ether sulfate, 10% naphthalene sulfonate formaldehyde condensate, 5% sodium polycarboxylate, 3% stretching powder BX, 10% silica, and kaolin to make up the balance.

[0059] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0060] Preparation Example 6: 21% Compound I of Formula 1·pyridaben dispersible oil suspension (20:1)

[0061] Formula composition: 20% Formula I compound, 1% pyridaben, 5% alkylphenol polyoxyethylene ether, 10% sorbitan oleate polyoxyethylene ether, 1% calcium dodecylbenzene sulfonate, 1% sodium polycarboxylate, 1% naphthalene sulfonate formaldehyde condensate, 0.3% organic bentonite, soybean oil to make up the balance;

[0062] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0063] Preparation Example 7: 13.6% Compound I of Formula 1·pyridaben aqueous emulsion (16:1)

[0064] Formula composition: 12.8% Formula I compound, 0.8% pyridaben, 5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 3% polyoxyethylene sorbitan monooleate, 12% cyclohexanone, 0.2% xanthan gum, 5% ethylene glycol, 1% urea, 0.5% sodium benzoate, 0.05% silicone defoamer, deionized water to make up the balance;

[0065] Preparation method: According to the formula ratio, the active ingredients are completely dissolved in the solvent, and then an emulsifier is added to form an oil phase; the dispersant, antifreeze, deionized water, etc. are stirred evenly to form an aqueous phase; the oil phase is added to the aqueous phase and stirred evenly, and the particles are sheared at high speed until the particle size is qualified. After adding the defoamer, the mixture is stirred evenly to obtain the water emulsion product.

[0066] Preparation Example 8: 24% Formula I compound pyridaben suspension (15:1)

[0067] Formula composition: 22.5% Formula I compound, 1.5% abamectin, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% tristyrylphenol ethoxylated phosphate, 1% sodium polycarboxylate, 2% magnesium aluminum silicate, 0.2% carboxyethyl cellulose, 1% sodium sorbate, 5% glycerol, 0.5% silicone oil, deionized water to make up the balance;

[0068] Preparation method: Same as in preparation example 1.

[0069] Preparation Example 9: 18% Compound I·Azoxystrobin Emulsifiable Concentrate (8:1)

[0070] Formulation composition: 16% Formula I compound, 2% abamectin, 15% DMF, 15% Gelbert alcohol polyoxyethylene ether, 3% calcium dodecylbenzenesulfonate, 22% propylene carbonate, xylene to make up the balance;

[0071] Preparation method: Same as in preparation example 2.

[0072] Preparation Example 10: 32% Formula I compound pyridaben wettable powder (1:15)

[0073] Formula composition: 2% Formula I compound, 30% abamectin, 5% sodium lignosulfonate, 5% sodium alkylphenol polyoxyethylene ether sulfonate, 2% sodium naphthalenesulfonate, 2% sodium dodecyl sulfate, 8% attapulgite, and kaolin to make up the balance;

[0074] Preparation method: Same as in preparation example 3.

[0075] Preparation Example 11: 9% Formula I compound pyridaben microemulsion (1:8)

[0076] Formula composition: 1% Formula I compound, 8% azoxystrobin, 15% xylene, 20% cyclohexanone, 15% glycerol fatty acid ester polyoxyethylene ether, 5% EO-PO block copolymer, 1% sodium octylphenol polyoxyethylene ether sulfonate, 5% ethylene glycol, 0.1% silicone defoamer, deionized water to make up the balance;

[0077] Preparation method: Same as in preparation example 4.

[0078] Preparation Example 12: 24% Formula I compound pyridaben water-dispersible granules (1:3)

[0079] Formula composition: 6% Formula I compound, 18% abamectin, 7% sodium lignosulfonate, 5% sodium alkyl polyoxyethylene ether sulfonate, 2% BX splitting powder, 3% sodium polycarboxylate, 10% ammonium sulfate, and kaolin to make up the balance;

[0080] Preparation method: Same as in preparation example 5.

[0081] Preparation Example 13: 20% Formula I compound pyridaben dispersible oil suspension (4:1)

[0082] Formula composition: 16% Formula I compound, 4% azoxystrobin, 15% phenylethylphenol polyoxyethylene polyoxypropylene ether, 5% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 1% silica, 0.5% organobentonite, 20% 200# solvent oil, methyl oleate to make up the balance;

[0083] Preparation method: Same as in preparation example 6.

[0084] Preparation Example 14: 7% Compound I·Azoxystrobin aqueous emulsion (6:1)

[0085] Formula composition: 6% Formula I compound, 1% azoxystrobin, 2% arylphenol polyoxyethylene ether phosphate, 6% alkylaryl polyoxyethylene ether polyoxypropylene ether, 20% cyclohexanone, 5% ethylene glycol, 0.05% silicone defoamer, deionized water to make up the balance;

[0086] Preparation method: Same as in preparation example 7.

[0087] Indoor bioactivity test

[0088] Example 1: Indoor bioactivity assay of spider mites

[0089] Test Basis: The test was conducted in accordance with NY / T 1154.12-2008 "Indoor Biological Activity Tests of Pesticides - Insecticides - Part 12: Tetranychus Slide Immersion Method".

[0090] Experimental targets: Tetranychus cinnabarinus and Tetranychus citrus, with female adult mites selected.

[0091] Test agents: Compound of Formula I, pyridaben, and azoxystrobin technical grade;

[0092] Drug preparation: Dissolve the above raw materials in a suitable solvent, and then dilute with a 0.1% Tween 80 aqueous solution. Set up 5 mass concentration gradients according to the drug activity.

[0093] Experimental method: Cut double-sided tape into 2cm lengths and attach them to one end of a slide. Then select healthy mites and stick their backs onto the double-sided tape, 30 mites per slide. Place the slide in a container lined with a damp sponge, cover it, and place it at (25±1)℃ for 2 hours. After 2 hours, examine it under a microscope, remove dead and injured individuals, and replenish the slide to 30 mites.

[0094] Drug treatment: Immerse the glass slides in the respective treatment solutions, gently shake for 5 seconds, remove them, absorb excess solution with absorbent paper, place them in a white porcelain dish lined with a damp sponge, cover with a transparent plastic film, and observe them at (25±1)℃. Each treatment was repeated 4 times, and a blank control without drug treatment was set up.

[0095] Experimental investigation: The mortality of test insects was checked 48 hours after treatment. The total number of insects and the number of dead insects were recorded, and the mortality rate was calculated.

[0096] Data statistics and analysis:

[0097] Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula.

[0098]

[0099] In the formula:

[0100] P – Mortality rate, expressed as a percentage (%);

[0101] K represents the number of dead insects, in heads;

[0102] N represents the total number of insects treated, in units of heads.

[0103]

[0104] In the formula:

[0105] P1—Adjusted mortality rate, in percentage (%);

[0106] P t —The mortality rate is expressed as a percentage (%).

[0107] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0108] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0109] The LC was determined using the DPS statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0110] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0111]

[0112] In the formula:

[0113] ATI – Actual Measured Toxicity Index of Mixtures;

[0114] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0115] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0116] TTI = TI A ×P A +TI B ×P B

[0117] In the formula:

[0118] TTI – Theoretical Toxicity Index of Mixtures;

[0119] TI A —A. Toxicity index of drug A;

[0120] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0121] TI B —Toxicity index of drug B;

[0122] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0123]

[0124] In the formula:

[0125] CTC – Cotoxicity Coefficient;

[0126] ATI – Actual Measured Toxicity Index of Mixtures;

[0127] TTI – Theoretical Toxicity Index of Mixtures.

[0128] The co-toxicity coefficients (CTC) of the compound formulations ≥ 120 indicate a synergistic effect; CTC ≤ 80 indicate an antagonistic effect; and 80 < CTC < 120 indicate an additive effect. Indoor activity tests are shown in the table below:

[0129] Table 1. Results of indoor bioactivity tests of compound I and azithromycin on Tetranychus cinnabarinus.

[0130]

[0131]

[0132] Table 2 shows the results of indoor bioactivity tests of compound I combined with pyridaben on Tetranychus cinnabarinus.

[0133] Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Formula I compound y = 3.1850 + 1.4629x 0.9983 17.4049 / Pyridaben y = 5.4606 + 1.5668x 0.9959 0.5082 / 1:50 y = 5.5110 + 1.4749x 0.9977 0.4503 115.048 1:30 y = 5.5560 + 1.4564x 0.9851 0.4152 126.356 1:25 y = 5.5290 + 1.1748x 0.9943 0.3546 148.875 1:15 y = 5.6600 + 1.3420x 0.9970 0.3223 167.864 1:8 y = 5.7169 + 1.3918x 0.9928 0.3054 186.525 6:1 y = 4.8024 + 1.1532x 0.9854 1.4836 204.036 10:1 y = 4.5937 + 1.2053x 0.9884 2.1732 199.099 15:1 y = 4.1933 + 1.5040x 0.9990 3.4385 164.450 20:1 y = 4.1980 + 1.1537x 0.9891 4.9562 135.943 25:1 y = 3.9201 + 1.3890x 0.9979 5.9901 127.508 40:1 y = 4.0436 + 1.0730x 0.9933 7.7871 123.422

[0134] The results of indoor toxicological and biological activity tests (see Tables 1-2) showed that compound I, when combined with abamectin or pyridaben in a reasonable ratio, exhibited a significant synergistic effect against *Tetranychus carinata*. When the mass ratio of compound I to abamectin was 1:60–32:1, the co-toxicity coefficient against *Tetranychus carinata* was greater than 120, demonstrating a synergistic effect; when the mass ratio was 1:48–16:1, the co-toxicity coefficient against *Tetranychus carinata* was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:32–8:1, the co-toxicity coefficient against *Tetranychus carinata* was greater than 140, showing a remarkable synergistic effect. Similarly, when the mass ratio of compound I to pyridaben was 1:30–40:1, the co-toxicity coefficient against *Tetranychus carinata* was greater than 120, demonstrating a synergistic effect; and when the mass ratio was 1:25–20:1, the co-toxicity coefficient against *Tetranychus carinata* was greater than 130, showing a significant synergistic effect.

[0135] Table 3. Results of indoor bioactivity tests of compound I and azithromycin on *Pseudomonas citrus*.

[0136] Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Formula I compound y = 2.0848 + 1.5613x 0.9990 73.6361 / Azoxystrobin y = 4.5681 + 1.6474x 0.9984 1.8290 / 1:42 y = 4.7240 + 1.5515x 0.9944 1.5062 124.249 1:36 y = 4.7928 + 1.5243x 0.9975 1.3675 137.368 1:18 y = 4.8568 + 1.3903x 0.9951 1.2676 152.095 1:9 y = 4.8839 + 1.5263x 0.9993 1.1913 170.119 9:1 y = 3.7709 + 1.4395x 0.9960 7.1429 209.276 18:1 y = 3.3656 + 1.4720x 0.9971 12.8901 186.301 26:1 y = 3.2313 + 1.3976x 0.9980 18.4312 162.797 38:1 y = 2.9307 + 1.4682x 0.9995 25.6709 142.946 40:1 y = 2.8518 + 1.4471x 0.9988 30.5176 123.260

[0137] Table 4. Results of indoor bioactivity tests of compound I combined with pyridaben on *Pseudomonas citrus*.

[0138]

[0139] The results of indoor toxicity and bioactivity tests (see Tables 3-4) showed that compound I, when combined with abamectin or pyridaben in a reasonable ratio, had a significant synergistic effect against *Paecilomyces citrus*. When the mass ratio of compound I to abamectin was 1:42–40:1, the co-toxicity coefficient against *Paecilomyces citrus* was greater than 120, indicating a synergistic effect; when the mass ratio was 1:36–38:1, the co-toxicity coefficient against *Paecilomyces citrus* was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:18–38:1, the co-toxicity coefficient against *Paecilomyces citrus* was greater than 140, showing a remarkable synergistic effect. When the mass ratio of compound I to pyridaben is 1:32 to 40:1, the co-toxicity coefficient against *Paecilomyces citrus* is greater than 120, indicating a synergistic effect. When the mass ratio of compound I to pyridaben is 1:16 to 32:1, the co-toxicity coefficient against *Paecilomyces citrus* is greater than 130, indicating a significant synergistic effect. When the mass ratio of compound I to pyridaben is 1:8 to 16:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect.

[0140] Field efficacy trials

[0141] Example 2: Field trial for controlling strawberry spider mites

[0142] Experimental site: Strawberry greenhouse in Xinggongshan Village, Dongpu Town, Shaoxing City, Zhejiang Province. The spider mites in the experimental site were a mixed population of two-spotted spider mites and carmine spider mites.

[0143] Experimental crop: Strawberry (Red Beauty).

[0144] Experimental Design: The experiment included 5 treatments and a blank control, for a total of 6 treatments. Each treatment plot was 20 m². 2 Each treatment was repeated 4 times, and each experimental plot was randomly arranged into blocks.

[0145] Application method: The application time is March 11, 2023. The strawberry plants are sprayed evenly with a Gongnong-16 backpack sprayer.

[0146] Survey Methods: The initial mite population was assessed before pesticide application, and the number of live mites was assessed at 3, 7, and 14 days after application. Five sampling points were used in the plot, with five leaves fixed at each point, for a total of 25 leaves surveyed.

[0147] Methods for calculating drug efficacy:

[0148]

[0149]

[0150] Experimental Results and Analysis:

[0151] Table 5 Results of field trials for controlling strawberry spider mites

[0152]

[0153] Safety survey results: Throughout the experiment, observations were conducted periodically. The strawberry growth in each treatment area was consistent with that in the control area, as were other diseases, pests, and other organisms. No abnormalities were observed, indicating that the treatment dosages in this experiment were safe.

[0154] Efficacy survey results: Field efficacy test results show that the acaricidal composition of the present invention can be used to control strawberry spider mites. 14 days after application, the compound treatment group still maintained a good control effect.

[0155] Example 2: Field trial for controlling citrus mites

[0156] Experimental site: This experiment was conducted in Dagang Town, Nansha District, Guangzhou City, Guangdong Province. The experimental site has flat terrain, moderate fertility, and convenient irrigation and drainage.

[0157] Experimental crop: Citrus.

[0158] Experimental targets: Citrus rust mite and Citrus parrot mite.

[0159] Experimental design: The experiment consisted of 6 treatments, 4 replicates, and a total of 24 plots, with 2 citrus trees in each plot. The plots were randomly assigned to blocks, and isolation zones were set between them to prevent pesticide drift or other factors that could affect the experimental results.

[0160] Application time and method: The experiment was conducted once on August 30, 2023, using a Gongnong-16 backpack sprayer. The amount of pesticide applied was enough to moisten the leaves and fruits, with a slight dripping of pesticide from the leaves.

[0161] Experimental survey: Two trees were fixed in each plot for investigation. Two affected leaves from the same shoot stage were marked on the east, south, west, north, and center of each tree. Two fields of view were investigated, and the number of live mites was recorded. The initial mite population was investigated before pesticide application, and the number of live mites was counted at 3 days, 10 days, and 20 days after pesticide application.

[0162] Safety investigation: Throughout the efficacy test, the growth of citrus fruits in each drug-treated area and the water control area was observed periodically.

[0163] Methods for calculating drug efficacy:

[0164]

[0165]

[0166] Experimental Results and Analysis:

[0167] Table 6 Results of field trials for controlling citrus mites

[0168]

[0169] Safety: During the field trial, the leaf color and plant shape of citrus plants in different pesticide treatment areas and the blank control area were observed. No pesticide damage was found to the citrus plants, nor was any effect of promoting or inhibiting citrus growth observed. This indicates that the pesticides selected in this experiment are relatively safe for citrus trees at various dosages.

[0170] Field efficacy trials showed that the combination of compound I with abamectin or pyridaben had a good effect on citrus mites. The control efficacy against citrus mites was 89.59% and 92.85% 3 days after treatment, 94.09% and 95.68% 10 days after treatment, and 95.94% and 98.25% 20 days after treatment, all of which were significantly higher than the single-agent control.

[0171] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. A mite-killing composition, characterized in that, The active ingredients in the acaricide composition include active ingredient A and active ingredient B. Active ingredient A is a compound of formula I, and the structure of the compound of formula I is shown below: (Formula I), wherein the active ingredient B is azoxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:60 to 48:

1.

2. The acaricide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:60 to 40:

1.

3. The acaricide composition according to claim 1, characterized in that, The mass ratio of the compound of formula I to azoxystrobin is 1:60 to 38:

1.

4. The acaricide composition according to claim 3, characterized in that, The mass ratio of the compound of formula I to azoxystrobin is 1:42 to 38:1; preferably 1:36 to 38:1; more preferably 1:18 to 38:

1.

5. The acaricide composition according to claim 1, characterized in that, The total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the acaricide composition.

6. The acaricide composition according to claim 1, characterized in that, In addition to the active ingredient, the acaricide composition contains pesticide-permitted auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

7. The acaricide composition according to claim 1, characterized in that, The acaricide composition is prepared into a pesticide formulation, which may be a solid or liquid formulation.

8. The acaricide composition according to claim 7, characterized in that, The solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension, microemulsion, emulsifiable concentrate, water emulsion, or dispersible oil suspension.

9. The application of the acaricidal composition according to any one of claims 1-8 for the control of phytophagous mites.

10. The application according to claim 9, characterized in that, The phytophagous mites mentioned are pests belonging to the Tetranychidae and Erythrophagidae families.

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