Bioactive peptide-nano carrier synergistic anti-wrinkle essence as well as preparation method and use method thereof

Abstract

The invention relates to a bioactive peptide-nano carrier synergistic anti-wrinkle essence and a preparation method and a use method thereof, and belongs to the field of anti-wrinkle cosmetics, the bioactive peptide-nano carrier synergistic anti-wrinkle essence comprises the following substances by weight: 0.4-0.6 part of signal peptide, 1.8-2.2 parts of neurotransmitter inhibitory peptide, 0.3-0.5 part of carrier peptide, 2.7-3.3 parts of encapsulated liposome, 0.9-1.1 parts of nano hyaluronic acid, 0.2-0.4 part of propylene glycol, 5-6 parts of ethanol, and 16-20 parts of glycerin. And 60-66 parts of deionized water. The signal peptide can stimulate fibroblasts to proliferate collagen, the neurotransmitter inhibitory peptide can block muscle contraction signals, the carrier peptide can enhance transdermal absorption of active ingredients, and collagen regeneration can be continuously stimulated through the synergistic effect of the signal peptide, the neurotransmitter inhibitory peptide and the carrier peptide, so that the anti-wrinkle, anti-aging and anti-wrinkle effects are achieved; the wrapping lipidosome can wrap the active peptide, so that the active peptide can stably reach the corium layer and is slowly released, and the absorption efficiency of the active peptide is improved.

Description

Technical Field

[0001] This invention belongs to the field of anti-wrinkle cosmetics, specifically a bioactive peptide-nanocarrier synergistic anti-wrinkle essence and its preparation and application methods. Background Technology

[0002] As people age, the skin's self-repair ability gradually declines, and the synthesis of elastic and collagen fibers decreases, leading to sagging skin and wrinkles. Furthermore, ultraviolet radiation, unhealthy lifestyle habits, and environmental pollution can accelerate the skin aging process. Traditional anti-wrinkle technologies (such as retinol, hyaluronic acid, and botulinum toxin) suffer from poor permeability, high irritation, and short-lived effects, making it difficult to achieve ideal penetration and long-lasting results. Summary of the Invention

[0003] This invention provides a bioactive peptide-nanocarrier synergistic anti-wrinkle serum, its preparation method, and its usage method, in order to overcome the deficiencies in the prior art.

[0004] This invention is achieved through the following technical solution:

[0005] A bioactive peptide-nanocarrier synergistic anti-wrinkle essence comprises the following substances in parts by weight: 0.4-0.6 parts signal peptide, 1.8-2.2 parts neurotransmitter inhibitory peptide, 0.3-0.5 parts carrier peptide, 2.7-3.3 parts encapsulated liposomes, 0.9-1.1 parts nano hyaluronic acid, 0.2-0.4 parts propylene glycol, 5-6 parts ethanol, 16-20 parts glycerin, and 60-66 parts deionized water.

[0006] The above-mentioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence, wherein the signal peptide is palmitoyl pentapeptide-4 (Matrixyl TM ).

[0007] The aforementioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence contains a neurotransmitter inhibitory peptide, acetyl hexapeptide-8.

[0008] The above-mentioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence, wherein the carrier peptide is acetyl tetrapeptide-11.

[0009] The aforementioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence contains soybean lecithin as the encapsulated liposome.

[0010] The above-mentioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence has liposomes with a particle size of 80-120nm.

[0011] The above-mentioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence contains nano-hyaluronic acid with a particle size of 60-100nm.

[0012] A method for preparing a bioactive peptide-nanocarrier synergistic anti-wrinkle serum includes the following steps:

[0013] Step 1: Weigh out the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, encapsulated liposome, nano-hyaluronic acid, propylene glycol, ethanol, glycerol, and deionized water according to the specified ratio.

[0014] Step 2: Mix nano-hyaluronic acid, propylene glycol, ethanol, glycerin and deionized water at a temperature of 40-50℃ and a speed of 360-400r / min for 8-10 minutes, then cool to room temperature to obtain a mixed solvent;

[0015] Step 3: Then add the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, and encapsulated liposomes to the mixed solvent, and mix for 16-20 minutes at a temperature of 35-45℃ and a speed of 280-340r / min.

[0016] Step 4: After mixing, dispense the bioactive peptide-nanocarrier synergistic anti-wrinkle essence under aseptic conditions.

[0017] A method for using a bioactive peptide-nanocarrier synergistic anti-wrinkle essence: Use once daily. Apply 5-10ml of the bioactive peptide-nanocarrier synergistic anti-wrinkle essence evenly to the wrinkles on the face and gently massage for 1-2 minutes.

[0018] The above-described method of using a bioactive peptide-nanocarrier synergistic anti-wrinkle serum, wherein the duration of use is 10-50 days.

[0019] The advantages of this invention are: the signal peptide can stimulate fibroblasts to proliferate collagen / elastin, the neurotransmitter inhibitory peptide can block muscle contraction signals, and the carrier peptide can enhance the transdermal absorption of active ingredients. Through the synergistic effect of these three, collagen regeneration can be continuously stimulated, thereby achieving anti-wrinkle, anti-aging, and wrinkle-reducing effects. The encapsulated liposomes can encapsulate the signal peptide, neurotransmitter inhibitory peptide, and carrier peptide, enabling them to stably reach the dermis and be released slowly, thus improving absorption efficiency. Furthermore, the encapsulated liposomes have a nano-sized particle size, allowing them to quickly penetrate the stratum corneum and improve delivery efficiency. The nano-hyaluronic acid in this invention can fill wrinkles and provide rapid results. Simultaneously, this invention is non-irritating and suitable for sensitive skin. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] A bioactive peptide-nanocarrier synergistic anti-wrinkle essence comprises the following substances in parts by weight: 0.4-0.6 parts signal peptide, 1.8-2.2 parts neurotransmitter inhibitory peptide, 0.3-0.5 parts carrier peptide, 2.7-3.3 parts encapsulated liposomes, 0.9-1.1 parts nano hyaluronic acid, 0.2-0.4 parts propylene glycol, 5-6 parts ethanol, 16-20 parts glycerin, and 60-66 parts deionized water.

[0022] Preferably, the signal peptide described in this embodiment is palmitoyl pentapeptide-4 (Matrixyl) TM ).

[0023] Preferably, the neurotransmitter inhibitory peptide described in this embodiment is acetyl hexapeptide-8.

[0024] Preferably, the carrier peptide described in this embodiment is acetyl tetrapeptide-11.

[0025] Preferably, the encapsulated liposomes described in this embodiment are soybean lecithin.

[0026] Preferably, the particle size of the encapsulated liposomes described in this embodiment is 80-120 nm.

[0027] Preferably, the particle size of the nano-hyaluronic acid described in this embodiment is 60-100 nm.

[0028] A method for preparing a bioactive peptide-nanocarrier synergistic anti-wrinkle serum includes the following steps:

[0029] Step 1: Weigh out the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, encapsulated liposome, nano-hyaluronic acid, propylene glycol, ethanol, glycerol, and deionized water according to the specified ratio.

[0030] Step 2: Mix nano-hyaluronic acid, propylene glycol, ethanol, glycerin and deionized water at a temperature of 40-50℃ and a speed of 360-400r / min for 8-10 minutes, then cool to room temperature to obtain a mixed solvent;

[0031] Step 3: Then add the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, and encapsulated liposomes to the mixed solvent, and mix for 16-20 minutes at a temperature of 35-45℃ and a speed of 280-340r / min.

[0032] Step 4: After mixing, dispense the bioactive peptide-nanocarrier synergistic anti-wrinkle essence under aseptic conditions.

[0033] A method for using a bioactive peptide-nanocarrier synergistic anti-wrinkle essence: Use once daily. Apply 5-10ml of the bioactive peptide-nanocarrier synergistic anti-wrinkle essence evenly to the wrinkles on the face and gently massage for 1-2 minutes.

[0034] Preferably, the bioactive peptide-nanocarrier synergistic anti-wrinkle serum described in this embodiment is used for 10-50 days.

[0035] Example 1

[0036] Step 1: Weigh out the following components according to the specified ratio: palmitoyl pentapeptide - 40.4 parts, acetyl hexapeptide - 81.8 parts, acetyl tetrapeptide - 110.3 parts, soybean lecithin (particle size 80-120nm) - 2.7 parts, nano hyaluronic acid (particle size 60-100nm) - 0.9 parts, propylene glycol - 0.2 parts, ethanol - 5 parts, glycerol - 16 parts, and deionized water - 60 parts.

[0037] Step 2: Mix nano-hyaluronic acid, propylene glycol, ethanol, glycerin and deionized water at 40°C and 360 r / min for 10 min, then cool to room temperature to obtain a mixed solvent;

[0038] Step 3: Then add the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, and encapsulated liposomes to the mixed solvent, and mix for 20 min at 35°C and 280 r / min.

[0039] Step 4: After mixing, dispense the bioactive peptide-nanocarrier synergistic anti-wrinkle essence under aseptic conditions.

[0040] Example 2

[0041] Step 1: Weigh out the following components according to the specified ratio: palmitoyl pentapeptide - 40.6 parts, acetyl hexapeptide - 82.2 parts, acetyl tetrapeptide - 110.5 parts, soybean lecithin (particle size 80-120nm) - 3.3 parts, nano hyaluronic acid (particle size 60-100nm) - 1.1 parts, propylene glycol - 0.4 parts, ethanol - 6 parts, glycerol - 20 parts, and deionized water - 66 parts.

[0042] Step 2: Mix nano-hyaluronic acid, propylene glycol, ethanol, glycerin and deionized water at 50°C and 400 r / min for 8 min, then cool to room temperature to obtain a mixed solvent;

[0043] Step 3: Then add the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, and encapsulated liposomes to the mixed solvent, and mix for 16 min at 45°C and 340 r / min.

[0044] Step 4: After mixing, dispense the bioactive peptide-nanocarrier synergistic anti-wrinkle essence under aseptic conditions.

[0045] Example 3

[0046] Step 1: Weigh out the following components according to the specified ratio: palmitoyl pentapeptide - 40.5 parts, acetyl hexapeptide - 82.0 parts, acetyl tetrapeptide - 110.4 parts, soybean lecithin (particle size 80-120nm) - 3.0 parts, nano hyaluronic acid (particle size 60-100nm) - 1.0 part, propylene glycol - 0.3 parts, ethanol - 5.5 parts, glycerol - 18 parts, and deionized water - 63 parts.

[0047] Step 2: Mix nano-hyaluronic acid, propylene glycol, ethanol, glycerin and deionized water at 45°C and 380 r / min for 9 min, then cool to room temperature to obtain a mixed solvent;

[0048] Step 3: Then add the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, and encapsulated liposomes to the mixed solvent, and mix for 18 minutes at 40°C and 310 r / min.

[0049] Step 4: After mixing, dispense the bioactive peptide-nanocarrier synergistic anti-wrinkle essence under aseptic conditions.

[0050] The bioactive peptide-nanocarrier synergistic anti-wrinkle essence prepared in Examples 1-3 of this invention was used on internal employees and their families with crow's feet. The method of use is as follows: once a day, take 5-10ml of the bioactive peptide-nanocarrier synergistic anti-wrinkle essence and apply it evenly to the crow's feet area and gently massage for 1-2 minutes. Take photos before use and after four weeks of use to record the results. The comparison shows that after four weeks of use, the depth of crow's feet of all users was reduced by more than 50%. Moreover, the present invention can show a smoothing effect after 30 minutes of use. The advantages of this invention compared with retinol and botulinum toxin in the prior art are shown in Table 1 after continuous monthly observation of changes after discontinuation of use.

[0051]

[0052] Table 1

[0053] User 1

[0054] Ms. Wang, female, age 54, had a crow's feet wrinkle depth of 2.8 mm before use. After using the bioactive peptide-nanocarrier synergistic anti-wrinkle essence prepared in Example 1 of this invention for four weeks, the crow's feet wrinkle depth decreased to 1.2 mm. After six months of use, the crow's feet wrinkle depth was 1.6 mm, showing good persistence.

[0055] User 2

[0056] Ms. You, female, age 61, had a crow's feet wrinkle depth of 3.1 mm before use. After using the bioactive peptide-nanocarrier synergistic anti-wrinkle essence prepared in Example 2 of this invention for four weeks, the crow's feet wrinkle depth decreased to 1.5 mm. After six months of use, the crow's feet wrinkle depth was 2.1 mm, showing good persistence.

[0057] User 3

[0058] Ms. Bu, female, age 49, had a crow's feet wrinkle depth of 3.0 mm before use. After using the bioactive peptide-nanocarrier synergistic anti-wrinkle essence prepared in Example 3 of this invention for four weeks, the crow's feet wrinkle depth decreased to 1.3 mm. After six months of use, the crow's feet wrinkle depth was 1.5 mm, showing good persistence.

[0059] As shown in Table 1 and the trial group represented by users 1-3, the bioactive peptide-nanocarrier synergistic anti-wrinkle essence prepared by this invention can quickly relieve wrinkles and effectively play an anti-wrinkle role, thus achieving a long-term good anti-wrinkle effect.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A bioactive peptide-nanocarrier synergistic anti-wrinkle essence, characterized in that: It comprises the following substances in parts by weight: signal peptide 0.4-0.6 parts, neurotransmitter inhibitory peptide 1.8-2.2 parts, carrier peptide 0.3-0.5 parts, encapsulated liposomes 2.7-3.3 parts, nano-hyaluronic acid 0.9-1.1 parts, propylene glycol 0.2-0.4 parts, ethanol 5-6 parts, glycerol 16-20 parts, and deionized water 60-66 parts.

2. The bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 1, characterized in that: The signal peptide is palmitoyl pentapeptide-4.

3. The bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 1, characterized in that: The neurotransmitter inhibitory peptide mentioned is acetyl hexapeptide-8.

4. The bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 1, characterized in that: The carrier peptide is acetyl tetrapeptide-11.

5. The bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 1, characterized in that: The encapsulated liposomes are soybean lecithin.

6. The bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 1, characterized in that: The encapsulated liposomes have a particle size of 80-120 nm.

7. The bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 1, characterized in that: The particle size of the nano-hyaluronic acid is 60-100 nm.

8. A method for preparing a bioactive peptide-nanocarrier synergistic anti-wrinkle serum, characterized in that: Includes the following steps: Step 1: Weigh out the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, encapsulated liposome, nano-hyaluronic acid, propylene glycol, ethanol, glycerol, and deionized water according to the specified ratio. Step 2: Mix nano-hyaluronic acid, propylene glycol, ethanol, glycerin and deionized water at a temperature of 40-50℃ and a speed of 360-400r / min for 8-10 minutes, then cool to room temperature to obtain a mixed solvent; Step 3: Then add the signal peptide, neurotransmitter inhibitory peptide, carrier peptide, and encapsulated liposomes to the mixed solvent, and mix for 16-20 minutes at a temperature of 35-45℃ and a speed of 280-340r / min. Step 4: After mixing, dispense the bioactive peptide-nanocarrier synergistic anti-wrinkle essence under aseptic conditions.

9. A method of using a bioactive peptide-nanocarrier synergistic anti-wrinkle serum, characterized in that: Use once daily. Apply 5-10ml of the bioactive peptide-nanocarrier synergistic anti-wrinkle essence evenly to the wrinkles on your face and gently massage for 1-2 minutes.

10. The method of using the bioactive peptide-nanocarrier synergistic anti-wrinkle essence according to claim 9, characterized in that: The aforementioned bioactive peptide-nanocarrier synergistic anti-wrinkle essence is used for 10-50 days.

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