Albizia flower compound essential oil with sleep aiding and depression relieving functions and preparation method of albizia flower compound essential oil

Abstract

The invention provides albizia flower compound essential oil with sleep aiding and depression relieving functions and a preparation method of the albizia flower compound essential oil, and relates to the technical field of aromatherapy and natural product application. The compound essential oil takes an albizia flower low-temperature two-stage extract as a core carrier and is compounded with various functional essential oil components such as bitter orange leaf essential oil, FCF bergamot essential oil and Egypt nymphaea tetragona original essence, and the compound essential oil is prepared through three core innovations of albizia flower carrier synergistic technology, multi-channel nerve regulation design and safety strengthening design. The technical problems that existing sleep-aiding essential oil is single in effect and high in potential nerve irritation and the bioavailability of albizia flower flavonoid glycoside is low are solved. Experimental verification shows that the compound essential oil can shorten the sleep latency period by 52% and reduce the anxiety score by 47%, can cooperatively regulate the GABA energy system, 5-hydroxytryptamine secretion and hypothalamus-pituitary-adrenal axis through an olfaction-nerve reflex path, realizes efficient anxiety resistance, deep sleep promotion and emotion balance, and is suitable for people with various anxiety insomnia and emotional disorder.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of aromatherapy and natural product application, in particular to a compound essential oil with synergistic sleep-aiding and depression-relieving effects, taking low-temperature double-stage extract of Albizia julibrissin as the core carrier and compounding multiple functional essential oils, and simultaneously relates to a preparation method and application of the compound essential oil. BACKGROUND

[0002] With the acceleration of modern social life pace and the increase of mental stress, anxiety insomnia and emotional imbalance problems are increasingly common, and the market demand for related sleep-aiding and depression-relieving products continues to grow. Aromatherapy, as a natural and mild adjustment method, is widely used in this field, and essential oil products become one of the mainstream choices due to their rapid effect and convenient use.

[0003] However, the existing sleep-aiding essential oil products have the following significant technical defects: 1. Limited efficacy: Most products rely on a single active ingredient (such as linalool in lavender essential oil) and only target the GABA system or a single pathway of melatonin secretion, which cannot achieve the synergistic effect of anti-anxiety, sleep promotion, and emotional balance, and the adjustment effect on complex cause anxiety insomnia is not good.

[0004] 2. Potential safety risk: Some essential oils contain furanocoumarin components, which are photosensitive and can cause skin damage when exposed to ultraviolet light after use. Some essential oils contain eucalyptol and other nerve-stimulating components, which may cause nerve stimulation and exacerbate insomnia symptoms when added in excess.

[0005] 3. Low utilization efficiency of natural products: Albizia julibrissin, as a traditional medicinal and edible material, contains flavonoid glycosides (such as acacetin and apigenin) with clear liver-soothing and depression-relieving, sedative and tranquilizing potential. However, the traditional extraction method of Albizia julibrissin extract has the problem of serious loss of heat-sensitive flavonoid glycosides and low bioavailability of active ingredients, which makes it difficult to fully exert its efficacy.

[0006] In view of the above technical problems, it is urgent to develop a sleep-aiding and depression-relieving essential oil product based on multi-pathway synergistic regulation mechanism, with high efficacy, high safety, and high bioavailability, to solve the problems raised in the background art. SUMMARY

[0007] (I) Technical problems solved The purpose of the present application is to overcome the defects of single efficacy, insufficient safety of existing sleep-aiding essential oil, and low utilization efficiency of Albizia julibrissin flavonoid glycosides, and to provide an Albizia julibrissin compound essential oil with sleep-aiding and depression-relieving functions and a preparation method thereof. The compound essential oil takes low-temperature double-stage extract of Albizia julibrissin as the carrier and compounding multiple functional essential oils, achieving the synergistic effects of anti-anxiety, sleep promotion, and emotional balance through multi-pathway neural regulation, while having high safety and high bioavailability.

[0008] (II) Technical Solution To achieve the above object, the present application is implemented by the following technical solution: A compound Albizia julibrissin flower essential oil with sleep-aiding and depression-relieving functions, which is compounded with functional essential oil components in the following proportions based on 10 ml of Albizia julibrissin flower double-stage extract as base oil: The front note includes 3 drops of bitter orange leaf essential oil and 2 drops of FCF bergamot essential oil; The middle note includes 3 drops of Egyptian blue water lily essential oil, 1 drop of special grade ylang-ylang essential oil, 1 drop of real lavender essential oil and 1 drop of small flower jasmine essential oil; The back note includes 2 drops of olibanum essential oil and 1 drop of zero linaloe essential oil. The flavonoid glycoside content in the Albizia julibrissin flower double-stage extract is ≥1.2 mg / ml.

[0009] Further, the Albizia julibrissin flower double-stage extract is prepared by a low-temperature double-stage extraction process, and the specific steps are as follows: 1) Freeze-crush the dried Albizia julibrissin flowers in a-40℃ environment to destroy the cell wall structure; 2) Mix the freeze-crushed Albizia julibrissin flowers with avocado oil at a mass ratio of 1:5, and cyclically extract at 60℃ for 30 days; 3) Remove impurities from the extract liquid by vacuum filtration, and then enrich the effective components by molecular distillation technology to obtain the flavonoid glycoside, which has a 37% higher retention rate than the traditional extraction method.

[0010] Further, a molecular co-delivery system of Acacetin and Apigenin is formed in the compound essential oil, and the system can make the transdermal rate of the effective components increase by 50% compared with single component, which is detected by the Franz diffusion cell method.

[0011] Further, the FCF bergamot essential oil is treated by a furanocoumarin removal process, which meets the ISO4730 certification standard and has no photosensitivity, and the eucalyptol content in the compound essential oil is <0.1%, which is monitored in real time by GC-MS technology.

[0012] Further, the preparation method of the Albizia julibrissin flower compound essential oil with sleep-aiding and depression-relieving functions comprises the following steps: Step S1. Preparation of base oil The Albizia julibrissin flower double-stage extract is prepared by a low-temperature double-stage extraction process to ensure that the flavonoid glycoside content is ≥1.2 mg / ml; Step S2. Essential oil compounding In a 25℃ light-proof environment, the functional essential oils are added to the base oil layer by layer in the order of front note, middle note and back note according to the proportions; Step S3. Mixing and homogenization Use a magnetic stirrer to stir the mixture for 30 minutes at a speed of 300 rpm to ensure that the components are evenly dispersed. Step S4. Seal and store. After homogenization, the compound essential oil is placed in a brown glass container and stored in a cool, dry place under conditions of 15-25℃ and relative humidity <60%.

[0013] Furthermore, the specific operation of "adding layer by layer" in step S2 is as follows: First, add the top notes of bitter orange leaf essential oil and FCF bergamot essential oil, and stir for 5 minutes. Then add the middle notes of Egyptian blue water lily absolute, extra ylang-ylang essential oil, true lavender essential oil, and jasmine absolute, and continue stirring for 15 minutes. Finally, add the base notes of frankincense essential oil and tonka bean absolute, and stir for 10 minutes.

[0014] Furthermore, the sleep-aiding and mood-relieving product is used via acupoint-enhancing application, specifically as follows: 30 minutes before bedtime, apply 2-3 drops of compound essential oil to the Tanzhong acupoint (Ren meridian) and Neiguan acupoint (Pericardium meridian). After application, take 3 deep breaths, each of which involves inhaling for 4 seconds, holding your breath for 2 seconds, and exhaling for 6 seconds. According to microdialysis probe testing, this method of use can increase the transdermal penetration rate of active ingredients by 60%.

[0015] Furthermore, the compound essential oil achieves its sleep-aiding and mood-lifting effects through multi-pathway neuromodulation, specifically including: ① Rapid anti-anxiety pathway: Linalool acetate in bitter orange leaf essential oil inhibits sympathetic nerve excitation; ② Limbic system regulatory pathway: Terpineol in Egyptian blue water lily protoplast and active ingredients in mimosa flower extract synergistically activate the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus; ③ Sleep induction pathway: Coumarin in tonka bean protoporphyrin triggers melatonin secretion from the pineal gland through the olfactory cortex.

[0016] (III) Beneficial Effects This invention provides a compound essential oil of Albizia julibrissin flowers with sleep-aiding and mood-relieving functions, and its preparation method. It has the following beneficial effects: 1. This invention provides a compound essential oil of Albizia julibrissin flower with sleep-aiding and mood-relieving functions and its preparation method. Through the olfactory nerve reflex pathway of "rapid anti-anxiety in the early stage - emotional balance in the middle stage - deep sleep-promoting in the later stage", it is combined with multiple essential oils such as bitter orange leaf, blue water lily, and tonka bean to synergistically regulate the GABAergic system, 5-HT secretion and HPA axis. It can shorten the sleep latency, reduce anxiety scores and increase the proportion of slow wave sleep, thus solving the limitations of traditional single-component essential oils that "only help sleep but do not relieve mood" or "only relieve anxiety but do not promote sleep".

[0017] 2. The application provides a Sophora japonica compound essential oil with sleep-aiding and depression-relieving functions and a preparation method thereof, a low-temperature double-stage extraction process of Sophora japonica is created, flavonoid glycoside retention rate is increased by 37% compared with traditional extraction, a molecular co-delivery system of acacetin and apigenin is constructed, transdermal rate is increased by 50%, the industry pain point of low bioavailability of Sophora japonica flavonoid glycoside is solved, and natural active ingredients can efficiently play a role.

[0018] 3. The application provides a Sophora japonica compound essential oil with sleep-aiding and depression-relieving functions and a preparation method thereof, all formulas exclude photosensitivity risks, FCF furanocoumarin removal process is used for Fagara citri, nervous excitation components are strictly controlled, eucalyptol content is less than 0.1%, no skin irritation reaction is found in human patch test, and traditional essential oil safety hazards such as photosensitivity damage and nerve stimulation are avoided, and the compound essential oil is suitable for long-term use of people with different skin types. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the application will be clearly and completely described in combination with the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application. Embodiment 1

[0020] The application provides a Sophora japonica compound essential oil with sleep-aiding and depression-relieving functions, 10 ml Sophora japonica double-stage extract is used as base oil, and functional essential oil ingredients and dosages are shown in Table 1. Fragrance layer Essential oil name Addition amount (10 ml base oil) Core active ingredient Efficacy mechanism Front note Bitter orange leaf essential oil 3 drops Linalyl acetate Up-regulate GABA receptor activity, inhibit excessive release of neurotransmitters, and quickly relieve anxiety Front note FCF bergamot essential oil 2 drops Limonene Promote serotonin (5-HT) synthesis and improve mood; use furanocoumarin-free process, no photosensitivity Middle note Egyptian blue lotus essential oil 3 drops Terpineol Enhance the intensity of brain alpha waves and enhance the brain's relaxation state, laying the foundation for deep sleep Middle note Superior ylang-ylang essential oil 1 drop Benzyl benzoate Specifically improve anxiety insomnia and reduce nervousness when falling asleep Middle note Real lavender essential oil 1 drop Linalyl acetate Prolong slow wave sleep duration and improve sleep quality, verified by EEG brain wave monitoring Middle note Small flower jasmine essential oil 1 drop Phenethyl alcohol and indole Promote 5-HT secretion, improve mood, and relieve depression tendency in cooperation with bergamot essential oil Back note Frankincense essential oil 2 drops Alpha-pinene Regulate HPA axis function, reduce cortisol levels, and break the vicious cycle of "anxiety-insomnia" Back note Zero linden essential oil 1 drop Coumarin Promote the production of melatonin precursors through the olfactory-pineal pathway and induce natural sleep A molecular co-delivery system of acacetin and apigenin is formed in the compound essential oil, the system can increase the transdermal rate of effective components by 50% compared with single components by Franz diffusion cell method detection; FCF Fagara citri essential oil is treated by furanocoumarin removal process, and eucalyptol content in the compound essential oil is less than 0.1%.

[0021] The application provides a preparation method of the Sophora japonica compound essential oil with sleep-aiding and depression-relieving functions, and the method comprises the following steps: Step S1. Preparation of base oil Take 500g of dried Albizia julibrissin flower and place it in a -40℃ freeze dryer for 2h. Freeze and crush the flower to a particle size of 100-200 mesh. Mix the crushed flower with avocado oil at a mass ratio of 1:5, and load it into a sealed extraction tank. Recycle extraction in a 60℃ constant temperature water bath for 30 days (stir 2 times a day for 10min each time). After extraction, use a 0.22μm microporous filter to vacuum filter out impurities. Then, send the filtrate to a molecular distillation device (evaporation temperature 80℃, vacuum degree 10Pa) for enrichment. Collect the distillate to obtain the Albizia julibrissin flower double-stage extract, which has a flavonoid glycoside content ≥1.2mg / ml as confirmed by HPLC detection. Step S2. Essential oil compounding In a 25℃ light-proof operation room, take 10ml of the Albizia julibrissin flower double-stage extract and load it into a brown glass beaker. First, add 3 drops of bitter orange leaf essential oil and 2 drops of FCF bergamot essential oil, and stir for 5min with a magnetic stirrer. Then, add 3 drops of Egyptian blue lotus essential oil, 1 drop of top-grade ylang-ylang essential oil, 1 drop of real lavender essential oil, and 1 drop of small-flower jasmine essential oil, and continue stirring for 15min. Finally, add 2 drops of frankincense essential oil and 1 drop of ziricote essential oil, and stir for 10min to ensure uniform mixing of all ingredients. Step S3. Quality detection Take samples to detect the pH value (5.5-7.0), density (0.92-0.95g / cm³), and active ingredient content (linalyl acetate ≥0.3%, coumarin ≥0.1%) of the compound essential oil. Step S4. Sealed storage After passing the detection, load the compound essential oil into a brown glass dropper bottle, seal it, and store it in an environment with a temperature of 20℃ and a relative humidity of <60% for 12 months. Example 2:

[0022] The Albizia julibrissin flower compound essential oil is used in the preparation of a sleep-aiding and depression-relieving product. The sleep-aiding and depression-relieving product is used in an acupoint synergistic application method, which is as follows: 30min before sleep, take 2-3 drops of the compound essential oil and apply it to the Zhongwan acupoint (Ren Channel) and the Neiguan acupoint (Pericardium Channel). After application, perform 3 deep breaths, each with 4s of inhalation, 2s of breath holding, and 6s of exhalation. According to microdialysis probe detection, this usage can increase the transdermal rate of effective ingredients by 60%.

[0023] The compound essential oil achieves sleep-aiding and depression-relieving effects through multi-channel neural regulation, which includes the following: ① Rapid anti-anxiety channel: linalyl acetate in bitter orange leaf essential oil inhibits sympathetic nerve excitement (verified by heart rate variability test); ② Limbic system regulation channel: terpinen-4-ol in Egyptian blue lotus essential oil and active ingredients in Albizia julibrissin flower extract synergistically activate hippocampal brain-derived neurotrophic factor (BDNF) expression (verified by mouse model). ③Sleep induction pathway: Coumarin in zeroing lignans triggers pineal melatonin secretion through the olfactory cortex.

[0024] Efficacy comparison experiment: 1. Experimental design Select 60 patients with anxiety insomnia (meet the DSM-5 anxiety disorder diagnostic criteria, PSQI score ≥12 points, HAMA score ≥14 points), randomly divided into experimental group and control group, the experimental group uses the compound essential oil of the application, the control group uses 10% single real lavender essential oil, the base oil is sweet almond oil, 30 people in each group, continuous use for 28 days, once a day, apply at 30min before sleep.

[0025] 2. Detection index and result Sleep latency: assessed by PSQI scale, the sleep latency of the experimental group was shortened from an average of 65min to 31min, with a shortening rate of 52% (p<0.01), and that of the control group was shortened from 63min to 48min, with a shortening rate of 24% (p<0.05); Anxiety score: assessed by HAMA scale, the anxiety score of the experimental group was reduced from an average of 21 points to 11 points, with a reduction rate of 47% (p<0.05), and that of the control group was reduced from 20 points to 16 points, with a reduction rate of 20% (p>0.05); Sleep quality: monitored by EEG, the proportion of slow wave sleep time in the experimental group was increased from 18% to 32%, and that in the control group was increased from 17% to 22%, and the improvement effect of the experimental group was significantly better than that of the control group.

[0026] In this paper, the following points need attention: 1. The embodiments of the present disclosure only involve the structures involved in the embodiments of the present disclosure, and other structures can refer to the usual design.

[0027] 2. In the case of no conflict, the embodiments of the present disclosure and the features in the embodiments can be combined to obtain new embodiments.

[0028] Although the embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

Claims

1. A compound Albizia julibrissin flower essential oil with sleep-aiding and depression-relieving functions, characterized in that, Take 10 ml of acacia flower double-stage extract as base oil, and compound functional essential oil ingredients in the following proportions: The front note includes 3 drops of bitter orange leaf essential oil and 2 drops of FCF bergamot essential oil. The middle note includes 3 drops of Egyptian blue water lily crude essential oil, 1 drop of special grade ylang-ylang essential oil, 1 drop of real lavender essential oil, and 1 drop of small flower jasmine crude essential oil. The back note includes 2 drops of frankincense essential oil and 1 drop of zero lindora crude essential oil. The acacia flower double-stage extract contains flavonoid glycosides with a content of ≥1.2 mg / ml.

2. The Albizia julibrissin compound essential oil with sleep-aiding and depression-relieving functions according to claim 1, characterized in that, The acacia flower double-stage extract is prepared by a low-temperature double-stage extraction process, and the specific steps are as follows: 1) Freeze-crush dry acacia flowers in a -40℃ environment to destroy the cell wall structure. 2) Mix the freeze-crushed acacia flowers with avocado oil at a mass ratio of 1:5, and extract for 30 days at 60℃. 3) Filter the extracted liquid under vacuum to remove impurities, and then enrich the effective components by molecular distillation technology to obtain flavonoid glycosides, which have a 37% higher retention rate than traditional extraction methods.

3. The Albizia julibrissin compound essential oil with sleep-aiding and depression-relieving functions according to claim 1, characterized in that, The compound essential oil forms a molecular co-transport system of acacetin and apigenin, which can increase the transdermal rate of effective components by 50% compared with single components, as detected by the Franz diffusion cell method.

4. The Albizia julibrissin compound essential oil with sleep-aiding and depression-relieving functions according to claim 1, characterized in that, The FCF bergamot essential oil is treated by a furanocoumarin removal process, and the eucalyptol content in the compound essential oil is <0.1%.

5. The preparation method of the Albizia julibrissin compound essential oil with sleep-aiding and depression-relieving functions according to any one of claims 1-4, characterized in that, The steps include: Step S1. Preparation of base oil Prepare acacia flower double-stage extract by a low-temperature double-stage extraction process to ensure that the flavonoid glycoside content is ≥1.2 mg / ml. Step S2. Essential oil compounding At 25℃ in a dark environment, add each functional essential oil to the base oil in the order of front note, middle note, and back note. Step S3. Mixing and homogenization Use a magnetic stirrer to stir the mixture for 30 min at a speed of 300 r / min to ensure uniform dispersion of all components. Step S4. Sealed storage Store the homogenized compound essential oil in a brown glass container in a cool, dry place under the conditions of temperature 15-25℃ and relative humidity <60% after sealing.

6. The preparation method of Albizia julibrissin compound essential oil with sleep-aiding and depression-relieving functions according to claim 5, characterized in that, The specific operation of "layer-by-layer addition" in step S2 is as follows: First, add the bitter orange leaf essential oil and FCF bergamot essential oil of the front note, stir for 5 min, then add the Egyptian blue water lily crude essential oil, special grade ylang-ylang essential oil, real lavender essential oil, and small flower jasmine crude essential oil of the middle note, continue to stir for 15 min, and finally add the frankincense essential oil and zero lindora crude essential oil of the back note, and stir for 10 min.

7. The use of the Albizia julibrissin compound essential oil according to any one of claims 1-4 in the preparation of a sleep-aiding and depression-relieving product, characterized in that, The use method of the sleep-aiding and depression-relieving product is the acupoint synergistic application method, which is as follows: 30 min before sleep, apply 2-3 drops of compound essential oil to the Dazhong and Neiguan acupoints, and perform 3 deep breaths after application, with 4 s of inhalation, 2 s of breath holding, and 6 s of exhalation for each deep breath. Microdialysis probe detection shows that this use method can increase the transdermal rate of effective components by 60%.

8. The use of the Althea rosea compound essential oil according to claim 7 in the preparation of a sleep-aiding and depression-relieving product, characterized in that, The compound essential oil achieves sleep-aiding and depression-relieving effects through multi-channel neural regulation, which specifically includes: ① Rapid anti-anxiety channel: linalyl acetate in bitter orange leaf essential oil inhibits sympathetic nerve excitation; ② Limbic system regulation channel: terpinen-4-ol in Egyptian blue water lily crude essential oil and active ingredients in acacia flower extract synergistically activate hippocampal brain-derived neurotrophic factor expression; ③Sleep-inducing pathway: Coumarin in zerumbet alcohol triggers pineal melatonin secretion through the olfactory cortex.