Donkey-hide gelatin and spina date seed wine capable of improving sleep quality and preparation method thereof

Abstract

The invention belongs to the field of wine, and provides donkey-hide gelatin and spina date seed wine capable of improving sleep quality and a preparation method of the donkey-hide gelatin and spina date seed wine. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20-30 parts of poria cocos, 20-30 parts of pericarpium citri reticulatae, 20-30 parts of astragalus membranaceus, 30-40 parts of fried spina date seed, 15-25 parts of lily, 30-40 parts of radix ophiopogonis, 15-25 parts of radix rehmanniae recen, 15-25 parts of longan aril, 15-25 parts of lophatherum gracile, 15-25 parts of oyster, 15-25 parts of rose, 15-25 parts of Chinese yam, 15-25 parts of fructus gardeniae, 10-20 parts of colla corii asini and 10-20 parts of liquorice. The colla corii asini and spina date seed wine prepared by the invention can effectively shorten the sleep latency period and further improve the sleep quality, and has a better effect compared with melatonin tablets sold in the market at present.

Description

Technical Field

[0001] This invention belongs to the field of wine, specifically a wine made from jujube seeds and donkey-hide gelatin to improve sleep quality and its preparation method. Background Technology

[0002] Insomnia has become a global health problem. According to the World Health Organization (WHO), approximately 27% of the global population suffers from varying degrees of sleep disorders, with the insomnia rate among Chinese adults reaching as high as 38.2%, and showing a trend towards affecting younger people. Chronic insomnia can lead to weakened immunity, memory loss, mood disorders (such as anxiety and depression), and metabolic syndrome (such as diabetes and obesity), significantly increasing the medical burden and social costs.

[0003] Melatonin tablets are one of the more common sleep-improving medications on the market. Melatonin is a hormone secreted by the pineal gland in the human body, and it plays an important role in regulating the human body's circadian rhythm and sleep-wake cycle.

[0004] However, melatonin tablets have many limitations in practical applications. Melatonin tablets mainly focus on regulating the biological clock, and their effect on improving sleep problems caused by psychological stress, anxiety and other factors is often unsatisfactory.

[0005] To address the problems raised in the background art, those skilled in the art have proposed a method for preparing a jujube seed and donkey-hide gelatin wine that improves sleep quality. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention provides a jujube seed wine with donkey-hide gelatin for improving sleep quality and its preparation method, thereby solving the problem that melatonin tablets in the prior art often have unsatisfactory effects on improving sleep problems caused by psychological stress, anxiety, and other factors.

[0007] A wine made from donkey-hide gelatin and jujube seeds to improve sleep quality is made from the following ingredients by weight: 1000g wine base, 20-30 parts Poria cocos, 20-30 parts dried tangerine peel, 20-30 parts Astragalus membranaceus, 30-40 parts roasted jujube seeds, 15-25 parts lily bulb, 30-40 parts Ophiopogon japonicus, 15-25 parts Rehmannia glutinosa, 15-25 parts longan pulp, 15-25 parts Lophatherum gracile, 15-25 parts oyster shell, 15-25 parts rose petals, 15-25 parts Dioscorea opposita, 15-25 parts Gardenia jasminoides, 10-20 parts donkey-hide gelatin, and 10-20 parts licorice root.

[0008] Preferably, it is made from the following ingredients by weight: 1000g wine base liquid, 25 parts Poria cocos, 25 parts dried tangerine peel, 25 parts Astragalus membranaceus, 35 parts stir-fried jujube seed, 20 parts lily bulb, 35 parts Ophiopogon japonicus, 20 parts Rehmannia glutinosa, 20 parts longan pulp, 20 parts Lophatherum gracile, 20 parts oyster shell, 20 parts rose, 20 parts yam, 20 parts gardenia, 15 parts donkey-hide gelatin, and 15 parts licorice.

[0009] Preferably, the wine base is a dry red wine with a total phenol content ≥1800mg / L, an alcohol content of 12±1%vol, and a pH value of 3.5±0.3.

[0010] Preferably, the wine also includes 0.1-0.3% of a nano-sized magnesium aluminum silicate carrier with a specific surface area ≥600 m². 2 / g.

[0011] Another aspect of the present invention provides a method for preparing a jujube seed wine with donkey-hide gelatin that improves sleep quality, as described above, comprising the following steps:

[0012] S1. The donkey-hide gelatin is pulverized separately and then activated by a low-temperature plasma treatment device;

[0013] S2. The remaining raw materials are quick-frozen and crushed with liquid nitrogen and then ultra-finely pulverized to a particle size of ≤100 mesh.

[0014] S3. Mix the pulverized medicinal materials with the wine base liquid in a shear emulsifier to obtain a mixture;

[0015] S4. Add donkey-hide gelatin to the mixture and place it in a magnetic stirrer for magnetic stirring;

[0016] S5. Place the stirred mixture into a pulsed electric field for extraction;

[0017] S6. The mixture after pulsed electric field treatment is filtered through a 0.22μm ceramic membrane to remove solids and obtain clarified wine;

[0018] S7. After pasteurizing the wine, store it.

[0019] Preferably, in step 1, the power of the low-temperature plasma processor is 300W and the processing time is 2min.

[0020] Preferably, in step S2, the liquid nitrogen quick-freezing temperature is -196°C and the time is 5 minutes.

[0021] Preferably, in step S3, the rotation speed of the emulsifying shear machine is 8000 rpm and the mixing time is 5 min.

[0022] Preferably, in step S4, the rotation speed of the magnetic stirrer is controlled at 50 rpm, and the temperature is controlled at 23-27℃.

[0023] Preferably, in step S5, the field strength of the pulsed electric field is 20 kV / cm, the pulse width is 100 μs, and the frequency is 10 Hz.

[0024] Using wine base as a solvent, fat-soluble components (such as jujube seed oil and rose volatile oil) are extracted from medicinal materials. These components contain a small amount of melatonin precursor (tryptophan), which helps regulate the sleep cycle.

[0025] Poria can strengthen the spleen and calm the mind, promote diuresis and eliminate dampness, and improve insomnia caused by spleen deficiency and dampness. Poria polysaccharides can regulate the immune system and reduce the interference of inflammatory factors on sleep.

[0026] Dried tangerine peel can regulate qi and strengthen the spleen, dry dampness and resolve phlegm, and help improve insomnia caused by qi stagnation in the spleen and stomach (such as abdominal distension and belching). It contains hesperidin, nobiletin and other components, which can promote gastrointestinal motility, reduce indigestion at night, regulate vagal nerve tone, enhance parasympathetic nerve activity and promote relaxation.

[0027] Astragalus can replenish qi and raise yang, strengthen the exterior and stop sweating, enhance the body's immunity, and improve qi deficiency-type insomnia (such as fatigue and spontaneous sweating). Astragalus polysaccharides can activate macrophages, enhance immune defense during sleep, regulate the hypothalamic thermoregulatory center, and promote deep sleep.

[0028] Roasted jujube seeds contain jujube seed saponins and flavonoids, which can regulate the activity of GABA (γ-aminobutyric acid) receptors, enhance the effects of inhibitory neurotransmitters, and shorten the time to fall asleep.

[0029] Lily bulbs can calm the mind and soothe the nerves, moisten the lungs and relieve coughs. They are suitable for those suffering from restlessness, palpitations, insomnia and excessive dreaming. They contain lily saponins and colchicine, which can reduce the excitability of the cerebral cortex and relieve anxiety.

[0030] Ophiopogon japonicus can nourish yin and promote body fluid production, clear the mind and relieve irritability, and alleviate insomnia caused by yin deficiency and excessive fire (such as irritability and night sweats). Ophiopogon japonicus saponins can reduce the activity of the hypothalamus-pituitary-adrenal axis (HPA axis), reduce stress response, improve myocardial ischemia, and indirectly improve sleep quality.

[0031] Rehmannia glutinosa can clear heat and cool the blood, nourish yin and promote body fluid production. It is effective for insomnia caused by yin deficiency and internal heat (such as dry mouth and five-center heat). It contains ingredients such as citronol and rehmannia glycoside, which can inhibit excessive excitation of the central nervous system, regulate serum cortisol levels, and relieve anxiety.

[0032] Longan pulp can nourish the heart and spleen, replenish blood and calm the mind. It is effective for insomnia caused by deficiency of both heart and spleen (such as having many dreams, waking up easily, and feeling weak). It is rich in glucose, sucrose and B vitamins, which provide energy to the brain and improve neurasthenia. It also contains adenosine, which can promote sleep induction.

[0033] Lophatherum gracile can clear heat and relieve irritability, promote urination and relieve strangury, and help improve insomnia caused by excessive heart fire (such as sores in the mouth and tongue, and dark and scanty urine). It contains ingredients such as arundoin and imperata cylindrica, which can reduce the excitation of the body temperature regulation center, promote the excretion of metabolic waste in the body, and reduce the burden on the body.

[0034] Oysters can calm the nerves and nourish yin, making them suitable for insomnia caused by liver yang hyperactivity (such as dizziness, tinnitus, and irritability). They are rich in minerals such as calcium and magnesium, which can regulate neuromuscular excitability and promote relaxation. Oyster polysaccharides have antioxidant effects, protecting neurons from oxidative damage.

[0035] Roses can soothe the liver, relieve depression, promote blood circulation, and alleviate pain. They can also relieve insomnia caused by liver qi stagnation (such as chest tightness, rib pain, and low mood). Roses contain volatile oils such as citronellol and nerol, which can stimulate the olfactory nerves and transmit relaxation signals to the brain.

[0036] Yam can nourish the spleen and stomach, promote the production of body fluids and benefit the lungs, enhance the digestive function of the spleen and stomach, and promote drug absorption. Yam polysaccharides can repair the intestinal mucosal barrier, improve the utilization rate of nutrients, regulate the balance of intestinal flora, indirectly affect the function of the brain-gut axis, and improve sleep quality.

[0037] Gardenia can clear away internal heat and relieve symptoms such as irritability, insomnia, and night sweats caused by heat disturbing the mind.

[0038] Donkey-hide gelatin can nourish blood and yin, moisten dryness and stop bleeding. It is effective for insomnia caused by blood deficiency and chlorosis (such as pale complexion and palpitations). It is rich in collagen and amino acids, which can promote hemoglobin synthesis, improve blood supply to the brain, regulate serum ferritin levels, and relieve sleep disorders caused by anemia.

[0039] Licorice can harmonize various medicines, clear heat and detoxify, and alleviate the irritation of cold medicines in the prescription. Glycyrrhizic acid can inhibit gastric acid secretion and protect the gastric mucosa (especially for cold medicines such as Rehmannia glutinosa and Gardenia jasminoides). It contains glycyrrhetinic acid, which has a glucocorticoid-like effect and reduces the interference of inflammatory response on sleep.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] 1. The donkey-hide gelatin and jujube seed wine prepared by this invention achieves the effects of calming the mind, nourishing deficiency, clearing heat, and regulating the spleen through the combination of various materials. Among them, stir-fried jujube seeds, lily bulbs, and longan pulp regulate GABA and shorten the time to fall asleep; astragalus, donkey-hide gelatin, and longan pulp nourish qi and blood and improve insomnia caused by deficiency of both heart and spleen; ophiopogon japonicus, rehmannia glutinosa, and gardenia clear away internal heat and relieve insomnia caused by yin deficiency and fire excess; poria cocos, tangerine peel, and yam strengthen the spleen and remove dampness and improve insomnia caused by stagnation of spleen and stomach qi. At the same time, the donkey-hide gelatin and jujube seed wine prepared by this invention can effectively shorten the sleep latency period and further improve sleep quality, with better effects than melatonin tablets currently sold on the market.

[0042] 2. This invention utilizes the synergistic effect of Poria cocos, stir-fried jujube seed, and donkey-hide gelatin in the acidic environment of wine to increase the GABA receptor binding rate by 2.3 times. Attached Figure Description

[0043] Figure 1 This is a flowchart illustrating the preparation process of the donkey-hide gelatin and jujube seed wine according to the present invention. Detailed Implementation

[0044] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0045] Example 1: This invention provides a jujube seed and donkey-hide gelatin wine for improving sleep quality, made from the following ingredients by weight: 1000g wine base, 20-30 parts Poria cocos, 20-30 parts dried tangerine peel, 20-30 parts Astragalus membranaceus, 30-40 parts roasted jujube seed, 15-25 parts lily bulb, 30-40 parts Ophiopogon japonicus, 15-25 parts Rehmannia glutinosa, 15-25 parts longan pulp, 15-25 parts Lophatherum gracile, 15-25 parts oyster shell, 15-25 parts rose, 15-25 parts Dioscorea opposita, 15-25 parts Gardenia jasminoides, 10-20 parts donkey-hide gelatin, and 10-20 parts licorice root.

[0046] Furthermore, it is made from the following ingredients by weight: 1000g wine base, 25 parts Poria cocos, 25 parts dried tangerine peel, 25 parts Astragalus membranaceus, 35 parts stir-fried jujube seed, 20 parts lily bulb, 35 parts Ophiopogon japonicus, 20 parts Rehmannia glutinosa, 20 parts longan pulp, 20 parts Lophatherum gracile, 20 parts oyster shell, 20 parts rose, 20 parts yam, 20 parts gardenia, 15 parts donkey-hide gelatin, and 15 parts licorice.

[0047] Furthermore, the wine base liquid is a dry red wine with a total phenol content ≥1800mg / L, an alcohol content of 12±1%vol, and a pH value of 3.5±0.3.

[0048] Among these methods, controlling the alcohol content and pH range of wine can induce the formation of α-helical structures in collagen in donkey-hide gelatin, thereby increasing the dissolution rate of jujube seed saponins.

[0049] Furthermore, this also includes adding 0.1-0.3% of nano-sized magnesium aluminum silicate carrier to the wine, with a specific surface area ≥600m². 2 / g.

[0050] Among them, by adding nano-sized magnesium aluminum silicate, the porous structure of the carrier material can avoid the mutual antagonism between the components of donkey-hide gelatin and jujube seed, thus improving the stability of the compatibility.

[0051] like Figure 1 As shown:

[0052] A method for preparing a jujube seed and donkey-hide gelatin wine for improving sleep quality, as described above, includes the following steps:

[0053] S1. The donkey-hide gelatin is pulverized separately and then activated by a low-temperature plasma treatment device;

[0054] S2. The remaining raw materials are quick-frozen and crushed with liquid nitrogen and then ultra-finely pulverized to a particle size of ≤100 mesh.

[0055] S3. Mix the pulverized medicinal materials with the wine base liquid in a shear emulsifier to obtain a mixture;

[0056] S4. Add donkey-hide gelatin to the mixture and place it in a magnetic stirrer for magnetic stirring;

[0057] S5. Place the stirred mixture into a pulsed electric field for extraction;

[0058] S6. The mixture after pulsed electric field treatment is filtered through a 0.22μm ceramic membrane to remove solids and obtain clarified wine;

[0059] S7. After pasteurizing the wine, store it.

[0060] Furthermore, in step 1, the power of the low-temperature plasma processor is 300W, and the processing time is 2 minutes.

[0061] Among them, activating donkey-hide gelatin with low-temperature plasma can break down the tight structure of collagen in donkey-hide gelatin, form an α-helical structure, promote hemoglobin synthesis, thereby enhancing the blood-nourishing and yin-tonifying effects of donkey-hide gelatin and improving blood supply to the brain.

[0062] Furthermore, in step S2, the liquid nitrogen quick-freezing temperature is -196°C and the time is 5 minutes.

[0063] Among these methods, quick freezing with liquid nitrogen can instantly destroy the cell walls of medicinal materials, releasing more active ingredients.

[0064] Furthermore, in step S3, the rotation speed of the emulsifying shear machine is 8000 rpm, and the mixing time is 5 min.

[0065] Furthermore, in step S4, the rotation speed of the magnetic stirrer is controlled at 50 rpm, and the temperature is controlled at 23-27℃.

[0066] Furthermore, in step S5, the field strength of the pulsed electric field is 20kV / cm, the pulse width is 100μs, and the frequency is 10Hz.

[0067] Example 2:

[0068] A wine made from donkey-hide gelatin and jujube seeds to improve sleep quality is made from the following ingredients by weight: 1000g wine base, 20 parts Poria cocos, 20 parts dried tangerine peel, 20 parts Astragalus membranaceus, 30 parts roasted jujube seeds, 15 parts lily bulb, 30 parts Ophiopogon japonicus, 15 parts Rehmannia glutinosa, 15 parts longan pulp, 15 parts Lophatherum gracile, 15 parts oyster shell, 15 parts rose, 15 parts Dioscorea opposita, 15 parts Gardenia jasminoides, 10 parts donkey-hide gelatin, and 10 parts licorice.

[0069] Preparation method: Same as in Example 1.

[0070] Example 3:

[0071] A wine made from donkey-hide gelatin and jujube seeds to improve sleep quality is characterized by being made from the following ingredients by weight: 1000g wine base liquid, 30 parts Poria cocos, 30 parts dried tangerine peel, 30 parts Astragalus membranaceus, 40 parts roasted jujube seeds, 25 parts lily bulb, 40 parts Ophiopogon japonicus, 25 parts Rehmannia glutinosa, 25 parts longan pulp, 25 parts Lophatherum gracile, 25 parts oyster shell, 25 parts rose, 25 parts Dioscorea opposita, 25 parts Gardenia jasminoides, 20 parts donkey-hide gelatin, and 20 parts licorice root.

[0072] Preparation method: Same as in Example 1.

[0073] Experimental Example 1:

[0074] Thirty patients with insomnia were randomly divided into three groups (experimental group, control group 1, and control group 2). The experimental group took the product of Example 1 (50ml / day), control group 1 took commercially available melatonin tablets (3mg / day), and control group 2 took ordinary red wine (50ml / day). The intervention lasted for 4 weeks. After the intervention, the sleep latency, sleep quality, and side effects of the patients were statistically analyzed. The results are shown in the table below:

[0075]

[0076] As shown in the table above, the jujube seed and donkey-hide gelatin wine prepared by this invention achieves the effects of calming the mind, replenishing deficiency, clearing heat, and regulating the spleen through the combination of various materials. Among them, stir-fried jujube seeds, lily bulbs, and longan pulp regulate GABA and shorten the time to fall asleep; astragalus, donkey-hide gelatin, and longan pulp replenish qi and blood and improve insomnia caused by deficiency of both heart and spleen; ophiopogon japonicus, rehmannia glutinosa, and gardenia clear away internal heat and relieve insomnia caused by yin deficiency and fire excess; poria cocos, tangerine peel, and yam strengthen the spleen and remove dampness and improve insomnia caused by stagnation of spleen and stomach qi. At the same time, the wine prepared by this invention can effectively shorten the sleep latency period and further improve sleep quality, and has a better effect than melatonin tablets sold on the market.

[0077] Experiment Example 2

[0078] To verify the synergistic effect of Poria cocos, stir-fried jujube seed and donkey-hide gelatin in the acidic environment of wine.

[0079] Group testing:

[0080] Single-group subgroups: The GABA receptor binding rates of Poria cocos, stir-fried jujube seed, and donkey-hide gelatin in wine base liquid at pH 3.5 were tested separately.

[0081] Two-group comparisons were conducted: the GABA receptor binding rates of Poria cocos and stir-fried jujube seed, Poria cocos and donkey-hide gelatin, and stir-fried jujube seed and donkey-hide gelatin in wine base at pH 3.5.

[0082] The three components were: the GABA receptor binding rates of Poria cocos, stir-fried jujube seed, and donkey-hide gelatin in wine base liquid at pH 3.5.

[0083] The GABA receptor binding rates of each component, as determined by HPLC, are shown below:

[0084] Single-group subgroups: jujube seed saponin A (100% baseline), poria triterpenic acid (85%), and donkey-hide gelatin peptide (92%).

[0085] Two-group subgroups: Ziziphus jujuba seed + Poria cocos (180%), Ziziphus jujuba seed + donkey-hide gelatin (175%), Poria cocos + donkey-hide gelatin (110%)

[0086] Three groups: 230%.

[0087] As can be seen from the above, this invention achieves a synergistic effect between Poria cocos, stir-fried jujube seed, and donkey-hide gelatin in an acidic environment of wine, which can increase the GABA receptor binding rate by 2.3 times.

[0088] The embodiments of the present invention are given for the purposes of illustration and description. Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A Rehmannia glutinosa and Zizyphus jujuba wine for improving sleep quality, characterized by comprising: It is made of the following raw materials by weight: 1000g of grape wine base liquid, 20-30 parts of poria cocos, 20-30 parts of dried tangerine or orange peel, 20-30 parts of radix astragali, 30-40 parts of fried jujube seed, 15-25 parts of lily, 30-40 parts of radix ophiopogonis, 15-25 parts of radix rehmanniae, 15-25 parts of longan aril, 15-25 parts of lophatheri herba, 15-25 parts of mactra, 15-25 parts of rose, 15-25 parts of yam, 15-25 parts of gardenia, 10-20 parts of donkey-hide gelatin, and 10-20 parts of licorice.

2. The E-jiao and Wu Jiaonan grape wine for improving sleep quality according to claim 1, characterized in that: It is made of the following raw materials by weight: 1000g of grape wine base liquid, 25 parts of poria cocos, 25 parts of dried tangerine or orange peel, 25 parts of radix astragali, 35 parts of fried jujube seed, 20 parts of lily, 35 parts of radix ophiopogonis, 20 parts of radix rehmanniae, 20 parts of longan aril, 20 parts of lophatheri herba, 20 parts of mactra, 20 parts of rose, 20 parts of yam, 20 parts of gardenia, 15 parts of donkey-hide gelatin, and 15 parts of licorice.

3. The E-jiao and Wu-zhao-ling grape wine for improving sleep quality according to claim 1, wherein: The grape wine base liquid is dry red grape wine with a total phenol content of ≥1800mg / L, an alcohol content of 12±1%vol, and a pH value of 3.5±0.

3. ​ 4. The E-jiao and Wu-zhao-ling grape wine for improving sleep quality according to claim 1, wherein: Also included is the addition of 0.1-0.3% of nanoscale magnesium aluminum silicate carrier with a specific surface area > 600 m 2 / g to the wine. ​ 5. The preparation method of the sleep quality improving donkey-hide gelatin and jujube seed wine according to any one of claims 1-4, characterized in that: It comprises the following steps: S1, activating the donkey-hide gelatin after being separately crushed by a low-temperature plasma treatment instrument; S2, crushing the remaining raw materials after being rapidly frozen by liquid nitrogen and then super-micro-crushing them to a particle size of ≤100 mesh; S3, mixing the crushed medicinal materials and the grape wine base liquid in a shearing emulsifier to obtain a mixed liquid; S4, adding the donkey-hide gelatin into the mixed liquid and stirring it in a magnetic stirrer; S5, putting the stirred mixed liquid into a pulsed electric field for extraction; S6, filtering the mixed liquid after being treated by the pulsed electric field through a 0.22μm ceramic membrane to remove solid substances and obtain clear grape wine; S7, pasteurizing the grape wine and then storing it.

6. The method of claim 5, wherein the preparation of the E. juccata wine for improving sleep quality is characterized by: In step 1, the power of the low-temperature plasma treatment instrument is 300W and the treatment time is 2min.

7. The method of claim 5, wherein the preparation of the E. juccata wine for improving sleep quality is characterized by: In step S2, the temperature of the liquid nitrogen rapid freezing is -196℃ and the time is 5min.

8. The preparation method of the E. J. wine for improving sleep quality according to claim 5, characterized in that: In step S3, the rotating speed of the emulsifying shearing machine is 8000rpm and the mixing time is 5min.

9. The method of claim 5, wherein the preparation of the E. juccata wine for improving sleep quality is characterized by: In step S4, the rotating speed of the magnetic stirrer is controlled at 50rpm and the temperature is controlled at 23-27℃.

10. The method of claim 5, wherein the preparation of the E. juccata wine for improving sleep quality is characterized by: In step S5, the field strength of the pulsed electric field is 20kV / cm, the pulse width is 100μs, and the frequency is 10Hz.

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