Traditional Chinese medicine composition with effects of diminishing inflammation and relieving cough and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects and a preparation method. The traditional Chinese medicine composition provided by the invention comprises a main medicine and an auxiliary medicine, the main medicine is prepared from nauclea officinalis, radix stemonae and pericarpium citri reticulatae; wherein the weight part of the nauclea officinalis is greater than or equal to the total weight part of the radix stemonae and the pericarpium citri reticulatae; the adjuvants comprise a first adjuvant and a second adjuvant; the first adjuvant is platycodon grandiflorum; the second adjuvant is selected from one or more of ceylon leucas herb, radix asteris, liquorice and / or honey ephedra. The invention provides a new traditional Chinese medicine formula core component with a definite and efficient inflammation eliminating effect, the nauclea officinalis, the radix stemonae and the pericarpium citri reticulatae are used as main materials, respiratory tract infection inflammations such as trachitis, bronchitis and sphagitis and cough symptoms caused by the respiratory tract infection inflammations can be relieved on the basis of eliminating the inflammations, and meanwhile the traditional Chinese medicine composition has high safety.

Description

[0001] Priority Application This application claims priority to Chinese Invention Patent Application No. 2025101046778, filed on January 22, 2025, entitled “Traditional Chinese Medicine Composition with Anti-inflammatory and Cough-Relieving Effects and Preparation Method,” which is incorporated by reference in its entirety. TECHNICAL FIELD

[0002] The present application belongs to the technical field of traditional Chinese medicine, and specifically relates to a traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects and a preparation method. BACKGROUND

[0003] Upper respiratory tract infection is the most common infectious disease of the respiratory tract. Patients are not divided by age, gender, occupation and region; not only has strong infectivity, but also can cause serious complications. In recent years, due to complex environment and rapid variation of viruses and bacteria, upper respiratory tract infection has become a factor that seriously affects normal life and health of human beings. At present, the treatment of upper respiratory tract infection still depends more on small molecule chemical drugs, such as acetaminophen. However, long-term and large-dose use of acetaminophen can cause damage to liver and kidney. Therefore, the development of effective and safer traditional Chinese medicine preparations is the future development direction.

[0004] The patent with publication number CN104721670A and the title of “A Traditional Chinese Medicine Composition for Treating Laryngopharyngitis and a Preparation Method” discloses a composition including many components such as purple thistle, hot grass, honeysuckle, white flower snake grass, poria cocos, stemona, kurchi, hu tong tears, licorice, etc. This patent controls laryngopharyngitis by clearing heat and resolving toxins, detumescence and analgesia, but does not objectively give the control effect of this traditional Chinese medicine on inflammation.

[0005] In view of the above, it is necessary to propose a new traditional Chinese medicine prescription to meet the development of modern medicine. SUMMARY

[0006] Therefore, the purpose of the present application is to provide a traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects and a preparation method, to partially solve or alleviate the above-mentioned deficiencies in the prior art. The present application specifically adopts the following technical solutions.

[0007] The first aspect of the present application is to provide a traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects composed of main drugs and auxiliary drugs.

[0008] A traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects, the traditional Chinese medicine composition comprising main drugs and auxiliary drugs; the main drugs consisting of kurchi, stemona and dried tangerine or orange peel; wherein the weight parts of the kurchi is greater than or equal to the total weight parts of the stemona and the dried tangerine or orange peel; the auxiliary drugs comprising first auxiliary drugs and second auxiliary drugs; the first auxiliary drugs being balloon-flower rhizome; the second auxiliary drugs being selected from one or more of honeycomb grass, purple thistle, licorice and / or honey ephedra.

[0009] Further, the Nauclea includes Nauclea officinalis, Nauclea officinalis leaf and / or Nauclea officinalis extract.

[0010] Further, the main medicine of the traditional Chinese medicine composition is composed of Nauclea officinalis, Stachys floridus and Pericarpium Citri Reticulatae; wherein the weight of the Nauclea officinalis is equal to the total weight of the Stachys floridus and the Pericarpium Citri Reticulatae; the first auxiliary medicine is Platycodon grandiflorum; and the second auxiliary medicine is Mellitis herba.

[0011] Preferably, the weight ratio of the main medicine and the auxiliary medicine is 1:1.

[0012] Further, the main medicine of the traditional Chinese medicine composition is composed of Nauclea officinalis, Stachys floridus and Pericarpium Citri Reticulatae; wherein the weight of the Nauclea officinalis is greater than the total weight of the Stachys floridus and the Pericarpium Citri Reticulatae; the first auxiliary medicine is Platycodon grandiflorum; and the second auxiliary medicine includes Radix Pulsatillae, Glycyrrhiza uralensis and Ephedra sinica; preferably, the weights of the Platycodon grandiflorum, the Radix Pulsatillae, the Glycyrrhiza uralensis and the Ephedra sinica are close to or equal to each other.

[0013] Preferably, the weight ratio of the Platycodon grandiflorum, the Radix Pulsatillae, the Glycyrrhiza uralensis and the Ephedra sinica is 1:1:1:1.

[0014] Preferably, the traditional Chinese medicine composition is composed of Nauclea officinalis, Stachys floridus, Pericarpium Citri Reticulatae, Platycodon grandiflorum and Mellitis herba, and specifically includes the following materials in the weight parts: Nauclea officinalis 3-7 parts, Stachys floridus 2-5 parts, Pericarpium Citri Reticulatae 1-3 parts, Platycodon grandiflorum 4-7 parts and Mellitis herba 4-7 parts.

[0015] More preferably, the traditional Chinese medicine composition is composed of Nauclea officinalis, Stachys floridus, Pericarpium Citri Reticulatae, Platycodon grandiflorum and Mellitis herba, and specifically includes the following materials in the weight parts: Nauclea officinalis 4 parts, Stachys floridus 3 parts, Pericarpium Citri Reticulatae 1 part, Platycodon grandiflorum 4 parts and Mellitis herba 4 parts.

[0016] Preferably, the traditional Chinese medicine composition is composed of Nauclea officinalis, Stachys floridus, Pericarpium Citri Reticulatae, Platycodon grandiflorum, Radix Pulsatillae, Glycyrrhiza uralensis and Ephedra sinica, and specifically includes the following materials in the weight parts: Nauclea officinalis 10-40 parts, Stachys floridus 5-15 parts, Pericarpium Citri Reticulatae 10-30 parts, Platycodon grandiflorum 10-30 parts, Radix Pulsatillae 10-30 parts, Glycyrrhiza uralensis 10-30 parts and Ephedra sinica 10-30 parts.

[0017] More preferably, the traditional Chinese medicine composition is composed of Nauclea officinalis, Stachys floridus, Pericarpium Citri Reticulatae, Platycodon grandiflorum, Radix Pulsatillae, Glycyrrhiza uralensis and Ephedra sinica, and specifically includes the following materials in the weight parts: Nauclea officinalis 30 parts, Stachys floridus 9 parts, Pericarpium Citri Reticulatae 10 parts, Platycodon grandiflorum 10 parts, Radix Pulsatillae 10 parts, Glycyrrhiza uralensis 10 parts and Ephedra sinica 10 parts.

[0018] The second aspect of the present application is to provide a traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects and with a simplified composition.

[0019] A traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects, which is composed of Nauclea officinalis, Stemona sessilifolia and Citrus reticulata.

[0020] Preferably, the weight ratio of the main drug to the auxiliary drug is 100:4, 100:5, 100:6, 100:7, 100:8, 100:9 or 100:10.

[0021] Preferably, the weight ratio of Stemona sessilifolia to Citrus reticulata in the auxiliary drug is 2:1, 3:1, 3:2, 4:1 or 4:3.

[0022] Further, the Nauclea officinalis includes Nauclea officinalis medicinal materials, Nauclea officinalis leaves and / or Nauclea officinalis extracts.

[0023] In some preferred modes, 10 parts of Nauclea officinalis medicinal materials are extracted to obtain 1 part of Nauclea officinalis extract.

[0024] The third aspect of the present application is to provide a preparation method of a traditional Chinese medicine preparation with anti-inflammatory and cough-relieving effects.

[0025] The preparation method of the traditional Chinese medicine preparation with anti-inflammatory and cough-relieving effects comprises the following steps: S01: crushing and sieving the traditional Chinese medicinal materials in the traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects composed of the main drug and the auxiliary drug or the traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects with simplified components according to the prescription to obtain materials; S02: adding 8-20 times the volume of pure water to the materials, fully soaking, then extracting at 90-100 DEG C for 30-60 min, filtering to obtain the first extract; adding 8-10 times the volume of pure water to the materials again, continuing to extract at 90-100 DEG C for 60-100 min to obtain the second extract, filtering and combining the two extracts, concentrating, drying to obtain dry extract; S03: crushing the dry extract to obtain fine powder, adding a food science or pharmaceutically acceptable carrier and / or adjuvant, and preparing into a traditional Chinese medicine preparation.

[0026] The fourth aspect of the present application is to provide a traditional Chinese medicine preparation with anti-inflammatory and cough-relieving effects.

[0027] The traditional Chinese medicine preparation with anti-inflammatory and cough-relieving effects comprises the traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects composed of the main drug and the auxiliary drug or the traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects with simplified components.

[0028] Further, the dosage form of the traditional Chinese medicine preparation includes powder, tablet, pill, capsule, granule and / or oral solution.

[0029] Further, the components involved in the above-mentioned traditional Chinese medicine composition generally include medicinal materials or decoction pieces processed from medicinal materials.

[0030] On the basis of the above-mentioned traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects, three specific traditional Chinese medicine composition compounds are provided.

[0031] Formula 1: A traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects, which is composed of Radix Toxicodendri, Stemona and Pericarpium Citri Reticulatae; the Radix Toxicodendri is the main drug, and the Stemona and Pericarpium Citri Reticulatae are auxiliary drugs; the weight ratio of the main drug to the auxiliary drugs is 100:4.

[0032] Formula 2: A traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects, characterized in that the traditional Chinese medicine composition is composed of Radix Toxicodendri, Stemona, Pericarpium Citri Reticulatae, Platycodon and Herba Melicis.

[0033] Formula 3: A traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects, characterized in that the traditional Chinese medicine composition is composed of Radix Toxicodendri, Stemona, Pericarpium Citri Reticulatae, Platycodon, Radix Pulsatillae, Radix Glycyrrhizae and Honeyed Ephedra.

[0034] Specifically, preparation methods of the above-mentioned three specific traditional Chinese medicine composition compounds are provided.

[0035] Method 1: 100 kg of Radix Toxicodendri, 3 kg of Stemona and 1 kg of Pericarpium Citri Reticulatae are processed into fine powder. The Radix Toxicodendri is extracted with water, and is boiled twice, 2-3 h after the first boiling and 2 h after the second boiling. The filtrates are combined and concentrated to 20 L. 2 times of 95% ethanol is added for stirring, and is left to stand for 6-8 h. The precipitate is added with 2 times of 95% ethanol again, and is treated according to the previous process. The filtrate is filtered to remove the residue, and the ethanol is recovered. The concentration is continued to form a thick paste (about 5 kg). The fine powder of Stemona and Pericarpium Citri Reticulatae and 1.1 kg of starch are added for granulation, drying, addition of auxiliary materials, tabletting and coating to obtain the product.

[0036] Method 2: 4 kg of Herba Melici, 3 kg of Stemona, 4 kg of Platycodon, 1 kg of Pericarpium Citri Reticulatae and 4 kg of Radix Toxicodendri are boiled twice with water. 1-2 h after the first boiling and 1 h after the second boiling, the filtrates are combined and concentrated to 4 L. 2 times of 95% ethanol is added for stirring, and is left to stand for 6-8 h. The precipitate is added with 2 times of 95% ethanol again, and is treated according to the previous process. The filtrate is filtered to remove the residue, and the ethanol is recovered. The concentration is continued to form 1 L. The auxiliary materials are added to prepare a syrup.

[0037] Method 3: 30 kg of chunmu, 9 kg of baibu, 10 kg of chenpi, 10 kg of jiegeng, 10 kg of zhiwan, 10 kg of gancao and 10 kg of honey ephedra are decocted with water for 2 times. The first time is 1-2 hours after boiling, and the second time is 1 hour. The filtrate is combined and concentrated to 4L. 2 times of 95% ethanol is added for stirring. After 6-8 hours, filtration is performed. The precipitate is added with 2 times of 95% ethanol for the previous treatment. The residue is removed by filtration. The ethanol is recovered. The concentration is 1L. The excipients are added to prepare a syrup. A traditional Chinese medicine preparation with anti-inflammatory and cough-relieving effects includes the traditional Chinese medicine composition of any one of the preceding.

[0038] The dosage form of the traditional Chinese medicine preparation includes powder, tablet, pill, capsule, granule and / or oral solution.

[0039] Solution 1: Chunmu is the monarch drug, which is bitter and cold in nature, and is distributed in the lung and large intestine channels. It is a good product for clearing heat and resolving toxins, reducing swelling and relieving pain in the Lahu medicine. Baibu enters the lung channel and is used for treating cough and upper respiratory as a ministerial drug. Chenpi can reduce turbidity and yin to stop vomiting and belching, and can also remove stagnation and relieve fullness, open the chest and diaphragm, and sweep sputum and phlegm as an auxiliary drug.

[0040] Solution 2: Chunmu is the monarch drug, which is bitter and cold in nature, and is distributed in the lung and large intestine channels. It is a good product for clearing heat and resolving toxins, reducing swelling and relieving pain in the Lahu medicine. Baibu enters the lung channel and is used for treating cough and upper respiratory as a ministerial drug. Chenpi can reduce turbidity and yin to stop vomiting and belching, and can also remove stagnation and relieve fullness, open the chest and diaphragm, and sweep sputum and phlegm. The combination of chenpi and honey ephedra can stop cough and phlegm as an auxiliary drug. Jiegeng is pungent and is used for treating lung diseases, has the effect of clearing the throat and relieving the diaphragm, and can carry other drugs into the lung as a ministerial drug.

[0041] Solution 3: Chunmu is the monarch drug, which is bitter and cold in nature, and is distributed in the lung and large intestine channels. It is a good product for clearing heat and resolving toxins, reducing swelling and relieving pain in the Lahu medicine. Baibu enters the lung channel and is used for treating cough and upper respiratory, zhiwan is used for treating cough and upper respiratory, and honey ephedra is good at stopping cough and upper respiratory, which are all ministerial drugs. Chenpi can reduce turbidity and yin to stop vomiting and belching, and can also remove stagnation and relieve fullness, open the chest and diaphragm, and sweep sputum and phlegm. Jiegeng is pungent and is used for treating lung diseases, has the effect of clearing the throat and relieving the diaphragm, and can carry other drugs into the lung as an auxiliary drug. Gancao adjusts the drugs as a ministerial drug.

[0042] Beneficial technical effects: The application provides a new traditional Chinese medicine composition core component with clear and efficient inflammation elimination effect, taking chunmu, baibu and chenpi as the main components, which can realize the relief of respiratory tract infection inflammation such as tracheitis, bronchitis and pharyngolaryngitis and the cough symptoms caused by the inflammation on the basis of inflammation elimination, and has high safety.

[0043] The present application actually comprises three specific traditional Chinese medicine formulas: the first one only contains three ingredients of Dalbergia hupeana Hance, Stemona tuberosa Lour and Citrus reticulata, which has a simple formula and obvious effect. In the traditional Chinese medicine formula, Dalbergia hupeana Hance accounts for an absolute proportion, and comparative tests show that the expression of NO content, TNF-α and IL-6 can be significantly reduced by only compounding a relatively small amount of Stemona tuberosa Lour and Citrus reticulata on the basis of Dalbergia hupeana Hance, thereby inhibiting inflammation and having a better effect than pure Dalbergia hupeana Hance water extract, which proves that the new traditional Chinese medicine formula has better curative effect and value. The second one is based on Dalbergia hupeana Hance, Stemona tuberosa Lour and Citrus reticulata, and compounding Platycodon grandiflorum and Herba Hypochaeridis as adjuvants, which also has a simple formula and obvious effect. The third one is based on Dalbergia hupeana Hance, Stemona tuberosa Lour and Citrus reticulata, and compounding Platycodon grandiflorum, Radix Pulsatillae, Radix Glycyrrhizae and honeyed Ephedra as adjuvants, which has a better anti-inflammatory effect than the existing formula containing no Dalbergia hupeana Hance, Stemona tuberosa Lour and Citrus reticulata. In addition, the new traditional Chinese medicine formula provided by the present application can also be used for actual clinical treatment and has obvious effects of relieving inflammation and cough. BRIEF DESCRIPTION OF DRAWINGS

[0044] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. In all the drawings, similar elements or parts are generally identified by similar reference signs. In the drawings, each element or part is not necessarily drawn according to the actual proportion. Obviously, the drawings described below are some embodiments of the present application, and those skilled in the art can obtain other drawings according to these drawings without any creative labor.

[0045] Figure 1 The state of bronchus and lung tissue of an animal model in one of the embodiments of the present application. DETAILED DESCRIPTION

[0046] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without any creative labor are within the protection scope of the present application.

[0047] In this document, “and / or” includes any and all combinations of one or more of the associated items.

[0048] In this document, “a plurality of” means two or more, that is, it includes two, three, four, five, etc.

[0049] As used in this specification, the term "about", when used in reference to a particular value, typically means + / - 5% of the stated value, more typically + / - 4% of the stated value, more typically + / - 3% of the stated value, more typically + / - 2% of the stated value, even more typically + / - 1% of the stated value, and even more typically + / - 0.5% of the stated value.

[0050] In this specification, certain embodiments can be disclosed in one specific format in a range. It should be understood that such "in a range" description is merely for the convenience and brevity, and should not be interpreted as a rigid limitation to the disclosed range. Therefore, the description of the range should be considered to have disclosed all possible sub-ranges and individual numerical values within the range. For example, the description of the range 1-6 should be considered to have disclosed the sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as individual numbers within the range, such as 1, 2, 3, 4, 5, and 6. The above rule applies regardless of the breadth of the range.

[0051] Embodiment 1 This embodiment provides a first traditional Chinese medicine composition with anti-inflammatory and cough-relieving effects.

[0052] The first traditional Chinese medicine composition: 100 parts of Kadsurae Cacumen, 3 parts of Farfarae Flos, and 1 part of Citri Reticulatae Pericarpium. Optionally, the Kadsurae Cacumen in this embodiment can be replaced by Kadsurae Cacumen extract, and when the added material is Kadsurae Cacumen extract, the weight part added is about 10 parts.

[0053] This embodiment provides the above-mentioned first traditional Chinese medicine composition, its preparation method and effect verification. The preparation method includes pretreatment, extraction and post-treatment.

[0054] S01: Pretreatment: crush the raw materials of Kadsurae Cacumen, Farfarae Flos and Citri Reticulatae Pericarpium in the formula ratio to pass through a 40-60 mesh sieve to obtain the material.

[0055] S02: Extraction: add 10 times the volume of pure water to the material, soak for 30 min, then extract at 95-100°C for 60 min, filter to obtain the first extract, add 8 times the volume of pure water to the material again, continue to extract at 95-100°C for 90 min to obtain the second extract, filter and combine the two extracts, concentrate and dry to obtain the dry extract.

[0056] S03: Post-treatment: crush the dry extract to obtain fine powder, add appropriate amount of excipients and mix uniformly to obtain the target traditional Chinese medicine composition which helps to lower blood lipids.

[0057] Further add food or pharmaceutical processing acceptable excipients or adjuvants to the composition in this embodiment to prepare the target traditional Chinese medicine preparation.

[0058] The survival rate of LPS-induced RAW264.7 inflammatory model (classical inflammatory model) cells after administration, the content changes of NO, TNF-α and IL-6 in the cell supernatant were determined, and the pharmacodynamic comparison was carried out with the cortex euonymi water extract (100 parts of cortex euonymi water extract), and the results are shown in Tables 1-3.

[0059] Table 1 is the survival rate of cells after stimulation with lipopolysaccharide (LPS), which is generally considered to be low when the survival rate is less than 70%. The damage degree of the compound to RAW264.7 cells was detected by CCK8 experiment to evaluate the in vitro anti-inflammatory activity of the compound. After LPS stimulation, the cell viability of RAW264.7 cells decreased, and after LPS stimulation, the NO release amount and the expression amount of inflammatory mediator TNF-α were significantly increased. The LPS concentration of 1 μg / ml with a cell survival rate of about 75% was selected for subsequent pharmacodynamic experiments. Tables 2 and 3 show that compared with the LPS group, the first traditional Chinese medicine composition experimental group (the first composition) can significantly reduce the content of NO and the expression of TNF-α and IL-6. The above results show that the traditional Chinese medicine composition can inhibit the content of inflammatory factors in LPS-induced RAW264.7 cells, reduce the accumulation of pro-inflammatory factors in RAW264.7 cells, and improve the accumulation of anti-inflammatory factors in RAW264.7 cells, and has good in vitro anti-inflammatory activity; and the effect is significantly better than that of the cortex euonymi water extract. In addition, the cell survival experiment shows that the safety of the first composition is higher.

[0060] Table 1 LPS CCK-8 determination of cell experiment Table 2 CCK-8 determination of cell survival rate of the first traditional Chinese medicine composition Table 3 Pharmacodynamic determination of the first traditional Chinese medicine composition Example 2 The second traditional Chinese medicine composition provided in this embodiment has the effects of relieving inflammation and coughing.

[0061] The second traditional Chinese medicine composition: cortex euonymi 4 parts, stemona 3 parts, dried tangerine or orange peel 1 part, platycodon 4 parts, and honeycomb grass 4 parts.

[0062] This embodiment provides the above-mentioned second traditional Chinese medicine composition, its preparation method and effect verification. The preparation method includes pretreatment, extraction and post-treatment.

[0063] S01: Pretreatment: The raw materials of cortex euonymi, stemona, dried tangerine or orange peel, platycodon and honeycomb grass in the formula are crushed to pass through a 40-60 mesh sieve to obtain the material.

[0064] S02: Extraction: 10 times the weight of the material in pure water was added to the material, soaked for 30 min, then decocted and extracted at 95-100°C for 60 min, filtered to obtain the first extract, 8 times the weight of the material in pure water was added again, and the decoction and extraction were continued at 95-100°C for 90 min to obtain the second extract, the two extracts were combined, concentrated, and dried to obtain a dry extract.

[0065] S03: Post-treatment: The dry extract was crushed to obtain a fine powder, and an appropriate amount of excipient was added to obtain a target traditional Chinese medicine composition that helps lower blood lipids.

[0066] In the composition of the present embodiment, food or pharmaceutical processing acceptable excipients or adjuvants are further added to prepare the target traditional Chinese medicine preparation.

[0067] The effects of the survival rate of LPS-induced RAW264.7 inflammatory model (classical inflammatory model) cells, the content changes of NO, TNF-α, and IL-6 in the cell supernatant after administration were determined, and the pharmacodynamic comparison was made with the Nauclea diderreri water extract (100 parts of Nauclea diderreri water extract), and the results are shown in Tables 4-6.

[0068] Table 4 is the survival rate of cells after LPS stimulation. The damage degree of the compound to RAW264.7 cells was detected by CCK8 experiment to evaluate the in vitro anti-inflammatory activity of the compound. After LPS stimulation, the cell viability of RAW264.7 cells decreased, and after LPS stimulation, the NO release amount and the expression amount of inflammatory mediator TNF-α were significantly increased. The LPS concentration of 1 μg / ml with a cell survival rate of about 75% was selected for subsequent pharmacodynamic experiments. Tables 5 and 6 show that compared with the LPS group, the second traditional Chinese medicine composition experimental group (the second composition) can significantly reduce the content of NO and the expression of TNF-α and IL-6. The above results show that the traditional Chinese medicine composition can inhibit the content of inflammatory factors in LPS-induced RAW264.7 cells, reduce the accumulation of pro-inflammatory factors in RAW264.7 cells, and improve the accumulation of anti-inflammatory factors in RAW264.7 cells, and has good in vitro anti-inflammatory activity; and the effect is significantly better than that of the Nauclea diderreri water extract. In addition, the cell survival experiment shows that the safety of the second composition is higher.

[0069] Table 4 LPS CCK-8 determination of cell experiment Table 5 CCK-8 determination of cell survival rate of the second traditional Chinese medicine composition Table 6 Pharmacodynamic determination of the second traditional Chinese medicine composition Example 3 The third Chinese medicine composition provided in the embodiment has anti-inflammatory and cough-relieving effects.

[0070] The third Chinese medicine composition comprises 30 parts of Cortex Toxicodendri, 9 parts of Radix Stachydis, 10 parts of Pericarpium Citri Reticulatae, 10 parts of Rhizoma Platycodi, 10 parts of Radix Pseudoginseng, 10 parts of Radix Glycyrrhizae, and 10 parts of Herba Ephedrae.

[0071] The third Chinese medicine composition provided in the embodiment and a preparation method and effect verification thereof are provided. The preparation method comprises pretreatment, extraction, and post-treatment.

[0072] S01: Pretreatment: The raw materials of Rhizoma Platycodi, Radix Pseudoginseng, Radix Stachydis, Radix Glycyrrhizae, Pericarpium Citri Reticulatae, Cortex Toxicodendri, and Herba Ephedrae in the formula are crushed to pass through a 40-60 mesh sieve to obtain the material.

[0073] S02: Extraction: 10 times the volume of pure water based on the weight of the material is added to the material, and the material is soaked for 30 min. Then, the material is decocted and extracted at 95-100°C for 60 min to obtain the first extract. 8 times the volume of pure water based on the weight of the material is added again, and the material is continuously decocted and extracted at 95-100°C for 90 min to obtain the second extract. The two extracts are combined, concentrated, and dried to obtain the dry extract.

[0074] S03: Post-treatment: The dry extract is crushed to obtain fine powder, and appropriate excipients are added to obtain the target Chinese medicine composition that helps reduce blood lipids.

[0075] In the composition of the embodiment, food or pharmaceutical processing acceptable excipients or adjuvants are further added to prepare the target Chinese medicine preparation.

[0076] The effects of the composition on the survival rate of LPS-induced RAW264.7 inflammatory model (a classical inflammatory model) cells, the content changes of NO, TNF-α, and IL-6 in the cell supernatant after administration are determined, and the pharmacodynamic comparison with the existing formula is performed. The results are shown in Tables 7-9.

[0077] Table 7 is the survival rate of cells after stimulation with LPS. The damage degree of the compound to RAW264.7 cells was detected by CCK8 experiment, and the in vitro anti-inflammatory activity of the compound was evaluated. After LPS stimulation, the cell viability of RAW264.7 cells decreased, and after LPS stimulation, the NO release amount and the expression amount of inflammatory mediator TNF-α of RAW264.7 cells were significantly increased. The LPS concentration of 1 μg / ml with a cell survival rate of about 75% was selected for subsequent pharmacodynamic experiment. Tables 8 and 9 show that compared with the LPS group, the third traditional Chinese medicine composition experimental group (the third composition) can significantly reduce the content of NO and the expression of TNF-α and IL-6. The above results show that the traditional Chinese medicine composition can inhibit the content of inflammatory factors in LPS-induced RAW264.7 cells, reduce the accumulation of pro-inflammatory factors in RAW264.7 cells, and improve the accumulation of anti-inflammatory factors in RAW264.7 cells, and has good in vitro anti-inflammatory activity. In addition, the cell survival experiment shows that the safety of the third composition is higher.

[0078] The existing formula adopted by the present application is: 8 parts of Platycodon grandiflorum, 8 parts of Schizonepeta, 8 parts of Allium bidentatum, 8 parts of Stachys asiatica, 8 parts of Reineckea, 3 parts of Glycyrrhiza, and 4 parts of Pericarpium Citri Reticulatae. The results prove that the third traditional Chinese medicine composition has better ability to inhibit inflammatory factors than the existing formula. Compared with the following, the third traditional Chinese medicine composition can more significantly reduce the content of NO and the expression of TNF-α and IL-6, and has better pharmacodynamic effect.

[0079] Table 7 LPS CCK-8 determination of cell experiment Table 8 CCK-8 determination of cell survival rate of the third traditional Chinese medicine composition Table 9 Pharmacodynamic determination of the third traditional Chinese medicine composition Example 4 This example provides animal experiment verification of the traditional Chinese medicine composition of example 1 and example 3.

[0080] The mice were randomly divided into groups, 6 in each group, and blank control group, model group, administration group 1 (100 parts of Chuan-Hong, 3 parts of Bai-Bu, 1 part of Chen-Pei), administration group 2 (30 parts of Chuan-Hong, 9 parts of Bai-Bu, 10 parts of Chen-Pei, 10 parts of Jie-Geng, 10 parts of Zi-Phuang, 10 parts of Gan-Cao, 10 parts of Mi-Ma-Huang) were set in turn. The mice were modeled by LPS-induced respiratory tract inflammation and administered, and then the content of inflammatory factors in the serum of the mice was detected. Compared with the blank group, the contents of inflammatory factors TNF-α, IL-6 and IL-8 in the model group were significantly increased, compared with the model group, the contents of inflammatory factors TNF-α and IL-6 in the positive drug group and administration groups 1 and 2 were significantly reduced, and the content of inflammatory factor IL-8 in the serum of the positive drug group and administration groups 1 and 2 was significantly reduced. The experimental results prove that administration groups 1 and 2 have a therapeutic effect on respiratory tract inflammation, and compared with the positive drug, the results show that administration groups 1 and 2 have a better therapeutic effect than the positive drug, and the experimental results are shown in Figure 1 . Figure 1 As the positive administration group 1 tissue, it can be seen that the tracheal and bronchial mucosa of the model group presents a state of hyperemia and edema, with white sticky secretions on the surface. The mucosal surface has a punctate bleeding condition. The volume of the diseased lung lobe increases, the weight increases, and the color dark red gradually changes to gray white. The lung, trachea and bronchus are significantly improved after administration.

[0081] Table 9 Inflammatory factor detection of mice in each group Note: compared with the blank group a p<0.05, b p<0.01; compared with the model group c p<0.05, d p<0.01 The embodiments of the application are described above in combination with the drawings, but the application is not limited to the specific embodiments described above, which are only illustrative and not restrictive, and those of ordinary skill in the art can make many forms under the inspiration of the application without departing from the purpose of the application and the scope protected by the claims.

Claims

1. A traditional Chinese medicine composition with anti-inflammatory and antitussive effects, characterized in that, The traditional Chinese medicine composition comprises a main medicine and an auxiliary medicine; the main medicine is composed of Cortex Ilicis Latifoliae, Stemona and Pericarpium Citri Reticulatae; wherein the weight of the Cortex Ilicis Latifoliae is greater than or equal to the total weight of the Stemona and the Pericarpium Citri Reticulatae; the auxiliary medicine comprises a first auxiliary medicine and a second auxiliary medicine; the first auxiliary medicine is Platycodon Grandiflorum; and the second auxiliary medicine is selected from one or more of Herba Melicis, Radix Pulsatillae, Radix Glycyrrhizae and / or Herba Ephedrae.

2. The traditional Chinese medicine composition of claim 1, characterized in that, The Cortex Ilicis Latifoliae comprises Cortex Ilicis Latifoliae medicinal materials, Cortex Ilicis Latifoliae leaves and / or Cortex Ilicis Latifoliae extracts.

3. The traditional Chinese medicine composition of claim 1, wherein, The main medicine of the traditional Chinese medicine composition is composed of Cortex Ilicis Latifoliae, Stemona and Pericarpium Citri Reticulatae; wherein the weight of the Cortex Ilicis Latifoliae is equal to the total weight of the Stemona and the Pericarpium Citri Reticulatae; the first auxiliary medicine is Platycodon Grandiflorum; and the second auxiliary medicine is Herba Melicis; and the weight ratio of the main medicine to the auxiliary medicine is 2-1:

1.

4. The traditional Chinese medicine composition of claim 1, wherein, The main medicine of the traditional Chinese medicine composition is composed of Cortex Ilicis Latifoliae, Stemona and Pericarpium Citri Reticulatae; wherein the weight of the Cortex Ilicis Latifoliae is greater than the total weight of the Stemona and the Pericarpium Citri Reticulatae; the first auxiliary medicine is Platycodon Grandiflorum; and the second auxiliary medicine comprises Radix Pulsatillae, Radix Glycyrrhizae and Herba Ephedrae; and the weight of the Platycodon Grandiflorum, the Radix Pulsatillae, the Radix Glycyrrhizae and the Herba Ephedrae is close to or equal to each other.

5. A traditional Chinese medicine composition having anti-inflammatory and anti-tussive effects, characterized in that, The traditional Chinese medicine composition is composed of Cortex Ilicis Latifoliae, Stemona and Pericarpium Citri Reticulatae; the Cortex Ilicis Latifoliae is a main medicine, and the Stemona and the Pericarpium Citri Reticulatae are auxiliary medicines; and the weight ratio of the main medicine to the auxiliary medicine is 100:4-10.

6. The traditional Chinese medicine composition of claim 5, wherein, The Cortex Ilicis Latifoliae comprises Cortex Ilicis Latifoliae medicinal materials, Cortex Ilicis Latifoliae leaves and / or Cortex Ilicis Latifoliae extracts.

7. The traditional Chinese medicine composition of claim 6, wherein, 10 parts of Cortex Ilicis Latifoliae medicinal materials extract 1 part of Cortex Ilicis Latifoliae extract.

8. A method for preparing a traditional Chinese medicine preparation with anti-inflammatory and cough-relieving effects, characterized in that, The method comprises the following steps: S01: crushing and sieving traditional Chinese medicinal materials in the traditional Chinese medicine composition of any one of claims 1-4 or the traditional Chinese medicine composition of any one of claims 5-7 according to the prescription to obtain a material; S02: adding 8-20 times the volume of pure water to the weight of the material, soaking thoroughly, then extracting at 90-100°C for 30-60 min, filtering to obtain a first extract; adding 8-10 times the volume of pure water to the weight of the material again, continuing to extract at 90-100°C for 60-100 min to obtain a second extract, filtering and combining the two extracts, concentrating, and drying to obtain a dry extract; S03: crushing the dry extract to obtain a fine powder, adding a food science or pharmaceutically acceptable carrier and / or adjuvant, and preparing a traditional Chinese medicine preparation.

9. A traditional Chinese medicine preparation with anti-inflammatory and anti-tussive effects, characterized in that, The traditional Chinese medicine preparation comprises the traditional Chinese medicine composition of any one of claims 1-4 or the traditional Chinese medicine composition of any one of claims 5-7.

10. The traditional Chinese medicine preparation of claim 9, characterized in that, The dosage form of the traditional Chinese medicine preparation comprises a powder, a tablet, a pill, a capsule, a granule and / or an oral solution.

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