Glass slide constant-temperature drying device

Abstract

The invention discloses a constant-temperature drying device for a glass slide, and belongs to the technical field of laboratory equipment. The device comprises a shell, a drying tray, a heat insulation plate, a heating film, an integrated controller and a double-temperature sensor. The drying tray is made of 6061 aluminum alloy and comprises five drying positions arranged in an array mode, and the center-to-center distance is 15 mm. The five polyimide flexible heating films are matched with the drying positions in a one-to-one mode, the power of each polyimide flexible heating film is 25 W, and 0-25 W stepless adjustment is achieved through PWM signals. The integrated controller is provided with an STM32H743 microcontroller and provided with a 16-bit ADC, and the sampling frequency is 20 Hz. The double PT1000 temperature sensors monitor in a distributed mode, the second sensor is embedded into the bottom of the center drying position to monitor the core temperature, and the first sensor is located on the edge to monitor the temperature difference. And by adopting an incremental PID algorithm, high-precision temperature control at + / -0.3 DEG C is realized, and the response time is less than or equal to 0.5 second. Compared with the prior art, the temperature control precision is improved by 85%, the energy consumption is reduced by 40%, five glass slides can be treated at the same time, and the pathological section manufacturing quality and efficiency are remarkably improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of laboratory equipment, in particular to a glass slide constant temperature drying device. BACKGROUND

[0002] Glass slide drying is a key step in the process of making pathological sections, processing cell smears and immunohistochemical experiments in pathological laboratories, medical laboratories and biological research institutions. In the process of pathological diagnosis, the tissue sections after staining treatment need to be dried at a specific temperature to remove residual moisture, fix the staining effect and ensure the long-term preservation quality of the sections. The accurate control of the drying temperature directly affects the stability of the staining effect, the integrity of the tissue structure and the clarity of the subsequent microscopic examination. If the temperature is too high, the tissue will be dehydrated excessively and the staining will fade. If the temperature is too low, the moisture cannot be completely removed, which affects the shelf life of the sections. Therefore, the temperature control accuracy of the glass slide drying device has an important influence on the quality of pathological diagnosis.

[0003] The existing glass slide drying devices mainly use electric heating plate heating or hot air circulation heating. The electric heating plate type drying device usually uses a single large heating plate, and multiple glass slides are placed directly on the surface of the heating plate for drying. This method has a obvious temperature gradient problem, with higher temperature in the center and lower temperature at the edges, resulting in inconsistent drying effects of glass slides at different positions. The hot air circulation type drying device achieves heating through hot air convection. Although the temperature uniformity is improved, it has the problems of slow heating speed, high energy consumption and large temperature fluctuation, and the flow of hot air can easily cause the displacement of unfixed sections. In addition, the temperature control of the existing device mainly uses simple on-off control or proportional control, and only one temperature sensor is usually used, which cannot accurately reflect the temperature distribution of the entire drying area, resulting in a temperature control accuracy of only about ±2℃, which cannot meet the requirements of precise pathological experiments.

[0004] In summary, the existing technology has the problems of low temperature control accuracy, uneven temperature distribution, slow response speed and low energy utilization efficiency, which seriously affect the quality of pathological section production and experimental efficiency. Especially in the case of batch processing of glass slides, the existing device cannot guarantee that each glass slide obtains the same drying conditions, resulting in inconsistency of experimental results. Therefore, it is urgent to develop a glass slide constant temperature drying device with high temperature control accuracy, uniform temperature distribution and fast response speed to meet the needs of modern pathological diagnosis and biomedical research for high-quality section production. SUMMARY

[0005] The present application aims to solve the technical problems of low temperature control precision, uneven temperature distribution and slow response speed of the existing slide drying device, and provides a slide constant temperature drying device to realize high-precision temperature control and fast temperature response.

[0006] To achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0007] A slide constant temperature drying device, comprising: a shell; a drying tray, which is slidingly inserted into the inside of the shell, and contains 5 drying positions arranged in an array, the center distance between adjacent drying positions is 15mm, and the drying tray is made of 6061 aluminum alloy material; a heat insulation plate, which is arranged inside the shell and below the drying tray; 5 heating films, which are respectively arranged on the bottom surface of the heat insulation plate, and each heating film is attached to the bottom of the corresponding drying position, the heating film is a polyimide flexible heating film, the rated power of a single heating film is 25W, the working voltage is DC 24V, and the edge of the heating film exceeds the edge of the corresponding drying position by 1mm; an integrated controller, which is arranged on the outer sidewall of the shell; a first temperature sensor, which is installed in the heat insulation layer between the 1st drying position and the 5th drying position, and is 2mm away from the edge of the drying position; and a second temperature sensor, which is embedded in the reserved hole at the bottom of the 3rd drying position.

[0008] Further, the heat insulation plate is a ceramic fiber heat insulation plate; and the heating film is fixedly attached to the bottom of the corresponding drying position by high-temperature resistant double-sided adhesive.

[0009] Further, the first temperature sensor and the second temperature sensor are both PT1000 platinum resistance temperature sensors, and are connected to the integrated controller through shielded twisted pair wires.

[0010] Further, the integrated controller comprises: an STM32H743 microcontroller, whose main frequency is 480MHz; a 16-bit ADC analog-digital converter, whose sampling frequency is 20Hz; a silicon controlled power regulation module, which is electrically connected with the 5 heating films; a 2.4-inch LED display screen, which is arranged on the surface of the integrated controller; and 3 physical keys, which are respectively a power on / off key, a temperature setting key and a drying timing key, and are arranged below the LED display screen.

[0011] Further, the integrated controller controls the power of the heating film through a PWM pulse width modulation signal, the frequency of the PWM signal is 1kHz, the duty cycle regulation range is 0-100%, and the power regulation range of the heating film is 0-25W.

[0012] Further, the temperature regulation range of the temperature setting key is 40-70℃, and the regulation step is 1℃; and the timing range of the drying timing key is 0-60 minutes, and the regulation step is 1 minute.

[0013] The present application has the following advantages:

[0014] 1. Adopting dual PT1000 temperature sensor distributed monitoring and incremental PID control algorithm, realizing temperature control accuracy of ±0.3℃, which is more than 85% higher than the accuracy of ±2℃ of the existing device, ensuring the consistency of slide drying effect; 5 independent heating films are matched with drying sites one by one, the design of the edge of the heating film exceeding the drying site by 1mm eliminates the temperature dead angle, cooperating with the array layout with a spacing of 15mm and the 6061 aluminum alloy high-thermal-conductivity tray, realizing uniform heating of the whole drying area, and the temperature difference is controlled within 0.5℃; the response time of PWM power regulation is less than or equal to 0.5 seconds, and the temperature sampling frequency of 20Hz ensures real-time monitoring, and the system can recover to the set value from temperature disturbance within 30 seconds, greatly improving the drying efficiency; using polyimide flexible heating film for direct lamination heating, cooperating with ceramic fiber heat insulation plate to reduce heat loss, which can reduce energy consumption by more than 40% compared with the hot air circulation method, and the power of a single drying site is only 25W; integrating LED display screen to display temperature and power information in real time, supporting wide range temperature adjustment of 40℃-70℃ and 0-60 minute timing function, equipped with multiple safety protection mechanisms such as over-temperature and overload, easy and reliable to operate; 5 independent drying sites can process 5 slides at the same time, and the temperature of each drying site is independently controllable, meeting the laboratory batch slide preparation demand and improving work efficiency.

[0015] The above description is only a summary of the technical scheme of the present application, in order to more clearly understand the technical means of the present application, the content of the specification can be implemented, and in order to make the above and other purposes, characteristics and advantages of the present application more obvious and easy to understand, the following preferred embodiments are described in detail below, and the accompanying drawings are described as follows. BRIEF DESCRIPTION OF DRAWINGS

[0016] Fig. 1 It is a perspective structural schematic diagram of a slide constant-temperature drying device proposed by the present application;

[0017] Fig. 2 It is a drying structure schematic diagram of a slide constant-temperature drying device proposed by the present application;

[0018] Fig. 3 It is an internal cross-sectional structure schematic diagram of a slide constant-temperature drying device proposed by the present application;

[0019] Fig. 4 It is a control system flow schematic diagram of a slide constant-temperature drying device proposed by the present application. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be described in detail below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all embodiments.

[0021] Example 1, Reference Figs. 1 to 4 The technical solutions of the present application will be described clearly and completely in conjunction with specific embodiments below, so that those skilled in the art can fully understand and implement the present application.

[0022] The present application provides a glass slide constant temperature drying device, as shown in the figure, which comprises a shell 1, a drying tray 2, a heat insulation plate 3, a heating film 4, an integrated controller 5, a first temperature sensor 6 and a second temperature sensor 7. The shell 1 serves as the external support structure of the entire device, and the drying tray 2 is slidably inserted inside. The drying tray 2 contains 5 independent drying positions arranged in an array. The center-to-center distance of each drying position is set to 15 mm, which can effectively prevent heat interference between adjacent drying positions and ensure the temperature independence of each drying position. The drying tray 2 is made of 6061 aluminum alloy material, which has excellent thermal conductivity of up to 167 W / (m·K) and good corrosion resistance, suitable for various chemical reagents in laboratory environment. The heat insulation plate 3 is arranged inside the shell 1 below the drying tray 2, used to isolate the heating area from the bottom of the shell, preventing heat conduction downward and causing energy loss. The heat insulation plate 3 is made of ceramic fiber insulation plate material, with a thermal conductivity of less than 0.03 W / (m·K) and a temperature resistance of up to 1000℃ or above, which can effectively maintain the heat concentration of the drying area.

[0023] The heating film 4 is arranged in 5 corresponding positions, one for each of the 5 drying positions. Each heating film 4 is arranged on the inner bottom surface of the heat insulation plate 3, tightly attached to the corresponding drying position below. The heating film 4 is made of polyimide (PI) flexible heating film, which has excellent high temperature resistance (can withstand temperature range of -200℃ to 300℃), good electrical insulation performance and excellent adhesion, and can perfectly adhere to the bottom of the drying tray 2. The rated power of each heating film 4 is set to 25W, and the working voltage is DC 24V. The heating film 4 is completely fixed to the bottom of the corresponding drying position using high-temperature resistant double-sided adhesive tape (temperature resistance grade ≥ 150℃). When adhering, the edge of the heating film 4 is ensured to exceed the edge of the drying position by 1mm, which can ensure uniform heating of the entire drying position, eliminate temperature dead angles and avoid insufficient temperature in the edge area of the glass slide. The heating film 4 supports stepless power adjustment of 0-25W, and the PWM (Pulse Width Modulation) signal output by the control module in the integrated controller 5 realizes precise power control. The PWM signal frequency is set to 1kHz, the duty cycle can be adjusted in the range of 0-100%, the response time is ≤0.5 seconds, which can quickly respond to temperature changes and compensate for power.

[0024] The integrated controller 5 is installed on the outer side wall of the shell 1, adopts a modular design, and internally integrates a high-precision temperature collector and a control circuit. The temperature collector is equipped with an STM32H743 microcontroller as the main control chip, which has a main frequency of up to 480MHz and is equipped with a 16-bit high-precision ADC (analog-to-digital converter). The sampling frequency of the ADC is set to 20Hz, i.e., 20 temperature data are collected per second, which can accurately collect the temperature signals of the first temperature sensor 6 and the second temperature sensor 7 in real time. The integrated controller 5 also integrates a silicon-controlled power regulation module, which receives the PWM control signal output by the microcontroller and adjusts the conduction angle of the silicon-controlled rectifier to accurately control the power of the five heating films 4. The surface of the integrated controller 5 is provided with a 2.4-inch LED display screen with a resolution of 320x240 pixels, which can display the average temperature of each drying position, the set target temperature, and the current heating power percentage in real time. Three physical buttons are provided below the display screen, namely the "power on / off" button, the "temperature setting" button, and the "drying timing" button. The temperature setting button supports target temperature adjustment in the range of 40-70℃, with a step size of 1℃ per press. The drying timing button supports timing setting in the range of 0-60 minutes, with a step size of 1 minute.

[0025] The first temperature sensor 6 and the second temperature sensor 7 both use PT1000 platinum resistance temperature sensors, which have a resistance value of 1000Ω at 0℃, high precision of ±0.15℃, and good long-term stability. The second temperature sensor 7 is embedded in the reserved hole at the bottom of the center drying position (the third drying position), with the probe directly contacting the aluminum alloy material of the drying tray 2, which can directly monitor the core temperature of the contact area of the glass slide and reflect the actual heating condition of the glass slide. The first temperature sensor 6 is installed inside the heat insulation layer between the first drying position and the fifth drying position, 2mm away from the edge of the drying position, for monitoring the temperature distribution of the edge area inside the device. By comparing the temperature difference between the two sensors, the temperature uniformity of the entire drying area can be evaluated to avoid local overheating or insufficient heating. Both temperature sensors are connected to the temperature collector using shielded twisted pair wires, with the shield layer grounded to effectively reduce the influence of electromagnetic interference on temperature signal transmission and ensure the accuracy and stability of temperature data.

[0026] The control algorithm adopts an incremental PID control strategy, and the PID parameters are set by experiment optimization: the proportional coefficient Kp=2.5, the integral coefficient Ki=0.8, and the differential coefficient Kd=0.3. The controller calculates the deviation value AT of the current temperature and the target temperature (default 60°C) in real time, and dynamically adjusts the power output of the heating film 4 according to the deviation value: when the temperature deviation AT>0.3°C (i.e. the current temperature is lower than 59.7°C), the controller increases the heating power, and the power increase is proportional to the deviation value, and the maximum can increase to 25W full power output; when the temperature deviation AT<-0.3°C (i.e. the current temperature is higher than 60.3°C), the controller reduces the heating power, and the power reduction is also proportional to the deviation value, and the minimum can be reduced to 0W complete stop heating; when the temperature deviation is within ±0.3°C (i.e. the temperature is between 59.7°C-60.3°C), the controller maintains the current power output unchanged, avoids frequent power adjustment causing temperature fluctuation, and ensures the stability of the temperature.

[0027] Example one

[0028] The complete working process of the device is illustrated by a specific use example as follows:

[0029] When the experimental personnel need to perform constant temperature drying treatment on the pathological section glass slide, first connect the device to the DC 24V power supply, press the "power on" key on the integrated controller 5 to start the device. The device enters the preheating stage, and the LED display screen displays the "preheating" word and the current real-time temperature, and the system automatically sets the target temperature to the default value of 60°C. The temperature collector immediately starts working, and controls the five heating films 4 to start heating at 25W full power at the same time. The microcontroller continuously collects the temperature data of the first temperature sensor 6 and the second temperature sensor 7 at a frequency of 20Hz (i.e. every 0.05 seconds), and calculates the average temperature value of the two sensors as the current temperature displayed on the LED screen in real time. During the preheating process, since the drying tray 2 is made of high thermal conductivity 6061 aluminum alloy material, the heat can be quickly and uniformly conducted to the entire drying area. Usually within 3-5 minutes, the average temperature can be raised from room temperature (about 25°C) to 59.5°C, at which time the system determines that the preheating is completed, the LED display screen displays "constant temperature ready", and the buzzer emits a short prompt sound, and the device automatically enters the constant temperature maintenance stage.

[0030] After entering the constant temperature drying stage, the experimenter places the slides to be dried on the top drying positions of the five drying trays 2 in turn and smoothly, and the slides are in direct contact with the aluminum alloy drying trays. Because the initial temperature of the slides is low, placing the slides will cause a short-term drop in the temperature of the drying positions. The second temperature sensor 7 can immediately detect this temperature change, for example, the temperature may drop from 60°C to 59.6°C. At this time, the PID control algorithm responds immediately, calculates the temperature deviation ΔT = 60-59.6 = 0.4°C > 0.3°C, and the controller increases the power of the heating film 4 from 12W in the maintenance state to 20W to speed up the temperature recovery speed. With the increase of the heating power, the temperature gradually rises, and when the temperature rises to 59.8°C, the deviation value decreases, and the power decreases to 16W accordingly; when the temperature continues to rise to 60.2°C, the deviation value becomes ΔT = 60-60.2 = -0.2°C, which is within the dead zone range of ±0.3°C, and the power is maintained at a level of about 10W at this time, realizing accurate and stable control of the temperature. The whole temperature regulation process responds quickly, and the set temperature can be restored and kept stable within 30 seconds after the slides are placed.

[0031] If the experimenter needs to adjust the drying temperature according to the specific experimental requirements, the "temperature setting" key can be used for adjustment, for example, some special dyed sections need to be dried at 65°C. Each press of the temperature setting key increases the target temperature by 1°C, and the LED screen displays the current set value in real time. After setting is completed, the system automatically confirms and starts executing the new temperature control after 3 seconds. If timed drying is required, the "drying timing" key can be used to set the drying time, for example, 10 minutes of timing is set, and the system starts counting down. The LED screen displays the remaining time. During the 10-minute drying process, the PID controller continues to work to ensure that the temperature is always stable within the set value ±0.3°C. When the timing time arrives, the device automatically reduces the heating power to 5W to enter the heat preservation state, preventing the slides from being over-dried, and at the same time, the buzzer emits 3 continuous prompt sounds, and the LED screen displays "drying complete", reminding the experimenter to take out the slides in time.

[0032] During the whole working process, the first temperature sensor 6 continuously monitors the edge area temperature and compares it with the data of the second temperature sensor 7. If the temperature difference between the two exceeds 1°C, the system will automatically adjust the power of the heating film of the edge drying positions (the first and the fifth) for differential heating compensation to ensure the temperature consistency of all drying positions. When the experiment is completed, the "shutdown" key is pressed, all heating films 4 are immediately powered off and stop heating, the device enters the heat dissipation mode, the LED screen displays "heat dissipation" and the current temperature value, and the fan assists in heat dissipation. When the temperature collector detects that the average temperature drops below 40°C, the system automatically cuts off the main power supply to complete the shutdown process.

[0033] In terms of safety protection, the system sets multiple protection mechanisms: when the detected temperature exceeds the set upper limit of 75℃, all heating film power is immediately cut off and an audible and light alarm is triggered; when the detected heating film current is abnormal (more than 120% of the rated value), it is determined as an overload failure, and the power is also cut off and an alarm is triggered; when the difference between the data of the two temperature sensors exceeds 5℃, it is determined as a sensor failure, the heating is stopped and maintenance is prompted. These protection mechanisms ensure the safety of the device in various abnormal situations.

[0034] Through the above specific embodiments, the slide constant-temperature drying device provided by the present application can realize high-precision temperature control of ±0.3℃, independent temperature management of 5 drying positions, fast temperature response, and perfect human-computer interaction and safety protection functions, fully meeting the technical requirements of laboratories on slide constant-temperature drying, and significantly improving the efficiency and quality of pathological section production.

[0035] The above merely describes the preferred embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art, according to the technical solution and inventive concept of the present application, can make equivalent replacements or changes within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A slide constant temperature drying device, characterized by, The utility model relates to a kind of drying device, including: Shell (1);Drying tray (2), the drying tray (2) is slidably inserted in the shell (1) inside, the drying tray (2) includes 5 drying sites in array layout, the center distance between adjacent drying sites is 15mm, the drying tray (2) is made of 6061 aluminum alloy material;Thermal insulation plate (3), is located in the shell (1) inside and is located below the drying tray (2);5 heating films (4) are respectively correspondingly set in the bottom surface of the thermal insulation plate (3), and each heating film (4) is attached to the bottom of corresponding drying site, the heating film (4) is polyimide flexible heating film, the rated power of single heating film (4) is 25W, working voltage is DC24V, the edge of the heating film (4) exceeds corresponding drying site edge 1mm;Integrated controller (5), is set to the external side wall of the shell (1);First temperature sensor (6), is installed in the thermal insulation layer between the first drying site and the fifth drying site, and the distance from drying site edge is 2mm;Second temperature sensor (7), is embedded in the reserved hole in the bottom of the third drying site.

2. The slide thermostatic drying device according to claim 1, wherein The thermal insulation plate (3) is ceramic fiber thermal insulation plate;The heating film (4) is fixedly attached to the bottom of corresponding drying site by high-temperature-resistant double-sided adhesive.

3. The slide thermostatic drying apparatus according to claim 1, wherein The first temperature sensor (6) and the second temperature sensor (7) are both PT1000 platinum resistance temperature sensors, and are connected to the integrated controller (5) by shielded twisted pair.

4. The slide thermostatic drying apparatus according to claim 1, wherein The integrated controller (5) includes: STM32H743 microcontroller, main frequency is 480MHz;16-bit ADC analog-digital converter, sampling frequency is 20Hz;Silicon-controlled power regulation module, is electrically connected with the 5 heating films (4);2.4 inch LED display screen, is set to the surface of the integrated controller (5);3 physical buttons, are respectively power on / off key, temperature setting key and drying timing key, are set below the LED display screen.

5. The slide thermostatic drying apparatus according to claim 4, wherein The integrated controller (5) controls the power of the heating film (4) by PWM pulse width modulation signal, the frequency of PWM signal is 1kHz, the duty ratio adjustment range is 0-100%, the power regulation range of the heating film (4) is 0-25W.

6. The slide thermostatic drying apparatus according to claim 4, wherein The temperature regulation range of the temperature setting key is 40-70 DEG C, and the regulation step is 1 DEG C;The timing range of the drying timing key is 0-60 minutes, and the regulation step is 1 minute.

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