Method for increasing xanthophyll content of marigold in harvesting period

Abstract

The invention relates to a technology for regulating and controlling the content of plant secondary metabolites, in particular to a research on the improvement of the content of Tagetes erecta L. xanthophyll and ester of the Tagetes erecta L. xanthophyll and ester of the Tagetes erecta L. xanthophyll. According to the present invention, the optimal concentration and the treatment mode of the marigold xanthophyll and the ester thereof by using the exogenous ABA are pointed out, the complete experimental method and the complete scheme of the application of the marigold exogenous ABA are provided, the application prospect is good, and the growth regulator does not produce the toxic effect on the plant and does not pollute the environment. According to the technical scheme, the problem of increasing the content of the marigold lutein and the ester thereof is solved, the content of the marigold lutein and the ester thereof treated by the exogenous ABA is increased from 11.57 g / kg to 20.13 g / kg, the content of the lutein and the ester thereof is greatly increased, and the method has important significance on increasing the production efficiency of the marigold lutein.

Description

Technical Field

[0001] This invention belongs to the field of lutein production, and specifically focuses on the research of technology to increase the content of lutein and its esters in marigolds. Background Technology

[0002] Marigolds are a major source of lutein internationally, as their petals are rich in lutein. Lutein has a wide range of uses, including food coloring, cosmetics, medicine, and livestock feed. It also plays an important role in preventing macular degeneration in humans. Lutein enhancers made from lutein extracted from marigolds can improve eyesight.

[0003] Due to the high economic value and huge market demand for lutein, agricultural production mainly involves manually harvesting fully open marigold flowers for saponification extraction. In my country, marigold breeding for pigments typically involves partial improvement of foreign varieties, and no breakthrough new varieties have been achieved. This has led to a situation of "difficulty in obtaining high-quality seeds" and "supply falling short of demand" in the industry, severely hindering its healthy development. Summary of the Invention

[0004] Based on the above, the method of the present invention aims to increase the content of lutein and its esters in marigolds, thereby improving production efficiency and enhancing the economic value of the product. To address the problems of "difficulty in obtaining high-quality seeds" and "supply falling short of demand" in the marigold lutein industry, this invention aims to provide a method for increasing the content of lutein and its esters, thereby improving production efficiency and enhancing economic benefits. To achieve the above-mentioned objective, this invention provides the following technical solution, comprising the following steps: (1) Selecting healthy, robust marigold plants in their flowering period for treatment. (2) Preparing an exogenous ABA solution before spraying marigolds with exogenous ABA. The steps include: weighing 264.32 mg of abscisic acid powder (98%, Coolaber) using an electronic balance, dissolving it in a small amount of anhydrous ethanol, and then adding distilled water to a final volume of 10 mL to obtain a 0.1 mol / L ABA stock solution. Using a pipette, 100 μL of the stock solution is taken and diluted to 100 mL with lignin sulfonate solution (2000 mg / L, Ufoxane 3A). (3) Marigold flowers 3 days before full bloom were selected and sprayed with a prepared ABA solution until the flowers were slightly dripping with water. This treatment was repeated every 24 hours for 3 consecutive times, serving as the treatment group. (4) Marigold flowers 3 days before full bloom were selected and sprayed with a lignin sulfonate solution until the flowers were slightly dripping with water. This treatment was repeated every 24 hours for 3 consecutive times, serving as the control group. (5) Marigolds treated with this solution should be protected from rain. (6) Lutein content was determined in lignin-sulfonate petals of marigolds treated with ABA solution and lignin sulfonate solution at 5, 12, and 48 hours. (7) The lutein content of marigold petals after different treatments was determined by organic solvent extraction and high performance liquid chromatography (HPLC). The differences in lutein content among the treatments were (as shown in the appendix). Figure 1 ). Attached Figure Description Figure 1 This is a comparison of lutein content in groups CK1, T1, CK2, T2, CK3, and T3 at different treatment concentrations for 5 hours, 12 hours, and 48 hours.

[0005] Figure 1As shown, CK1 consists of marigold petals treated with lignin sulfonate solution for 5 hours, with a lutein content of 10617 μg / g; T1 consists of marigold petals treated with exogenous ABA (concentration 100 μmol / L) for 5 hours, with a lutein content of 12222 μg / g; CK2 consists of marigold petals treated with lignin sulfonate solution for 12 hours, with a lutein content of 7420 μg / g; and T2 consists of marigold petals treated with exogenous ABA (concentration 100 μmol / L) for 5 hours. Marigold petals treated with BA (100 μmol / L) for 12 h had a lutein content of 11241 μg / g; CK3 consisted of marigold petals treated with lignin sulfonate solution for 48 h, with a lutein content of 11573 μg / g; and T3 consisted of marigold petals treated with exogenous ABA (abscisic acid) (100 μmol / L) for 48 h, with a lutein content of 20130 μg / g.

Claims

1. A method for increasing the lutein content of marigolds during the harvest period, characterized in that: Select healthy marigold plants that are flowering normally and plant them in suitable soil. When the ligulate petals are fully open, choose a sunny, windless day to spray them with exogenous ABA (abscisic acid).

2. The method for increasing the lutein content of marigolds during the harvest period as described in claim 1, characterized in that: The exogenous ABA concentration was 100 μmol / L, and the treatment time was 5, 12, and 48 h. Among them, the exogenous ABA treatment for 48 h increased the content of marigold lutein and its esters to 20.13 g / kg, which was 73.94% higher than that of the control group.

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