Traditional Chinese medicine composition for preventing hair loss and nourishing hair as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention relates to the field of daily necessities, and particularly discloses an anti-alopecia hair-growing traditional Chinese medicine composition as well as a preparation method and application thereof. The anti-hair-loss hair-growing traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 30 parts of walnut shell vinegar liquid, 10 to 30 parts of black silk fructus viticis, 10 to 20 parts of Chinese prickly ash, 10 to 20 parts of chrysanthemum stem leaves, 10 to 20 parts of aloe, 10 to 20 parts of fructus cnidii, 5 to 10 parts of fructus chebulae, 10 to 20 parts of black sesame, 5 to 10 parts of black grass seed oil, 5 to 10 parts of mutton fat seed rice washing water fermentation liquid, 1 to 5 parts of herba schizonepetae and 1 to 5 parts of plant essential oil. The traditional Chinese medicine composition can be used for preparing shampoo and has the advantages of being high in infiltration capacity, promoting hair regeneration and preventing hair loss.

Description

Technical Field

[0002] This application relates to the field of daily necessities technology, and more specifically, it relates to a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, its preparation method, and its application. Background Technology

[0004] Due to the fast pace, high stress, and relatively severe environmental pollution in modern society, hair loss is becoming increasingly prevalent among younger people, affecting more and more individuals. The pathology of hair loss is complex and the course of the disease is long, remaining a challenge for the medical community.

[0005] Hair originates from hair follicles, and the growth of hair follicles is cyclical, which can be divided into the anagen (growth) phase, catagen (transitional) phase, and telogen (resting) phase. Normal hair loss is mainly due to hair follicles being in the catagen and telogen phases. However, hair follicles are in dynamic equilibrium at each stage. For patients with hair loss, their hair follicles atrophy and cannot enter the anagen phase, which leads to the gradual thinning of hair.

[0006] Currently, there are many Chinese and Western medicines for treating hair loss. Among the Western medicines approved by the FDA are minoxidil and finasteride, but they all have shortcomings. Minoxidil is prone to relapse after discontinuation, and long-term use of finasteride can cause damage to the reproductive system. There are many Chinese medicines for treating hair loss, but the effects are often unsatisfactory, and they are expensive and require a very long course of treatment. Therefore, it is very valuable to find a hair growth agent that is easy to obtain, convenient to use, and has significant effects without rebound.

[0007] In the prior art, Chinese invention patent application number CN201810728202.6 discloses a traditional Chinese medicine composition and preparation for hair growth and care, in which the Chinese medicinal materials are Sanguisorba officinalis, Taraxacum mongolicum, Ginkgo biloba leaves, and Swertia japonica. The extract is prepared using a 95% alcohol extraction method and has the effect of treating seborrheic alopecia. However, its hair growth effect is not ideal. The main reason is that the hair growth components fail to effectively penetrate into the hair follicle cells. In response to the above-mentioned related technologies, the inventors believe there is an urgent need to provide a hair growth product with significant anti-hair loss and hair regrowth effects. Summary of the Invention

[0009] In order to improve the hair growth effect of traditional Chinese medicine composition, this application provides a hair loss prevention and hair growth traditional Chinese medicine composition, its preparation method and application.

[0010] In the first aspect, this application provides a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which adopts the following technical solution:

[0011] An anti - hair - loss and hair - growth traditional Chinese medicine composition, comprising the following raw materials in parts by weight: 10 - 30 parts of walnut shell vinegar solution, 10 - 30 parts of Wusiman grass, 10 - 20 parts of Chinese prickly ash, 10 - 20 parts of chrysanthemum stem and leaf, 10 - 20 parts of aloe vera, 10 - 20 parts of cnidium fruit, 5 - 10 parts of Terminalia chebula Retz., 10 - 20 parts of black sesame, 5 - 10 parts of black grass seed oil, 5 - 10 parts of fermented liquid of rice - washing water of sheep fat seed rice, 1 - 5 parts of Schizonepeta tenuifolia Briq., 1 - 5 parts of plant essential oil.

[0012] By adopting the above - mentioned technical solution, in Uyghur medicine, the theory of four major substances (fire, qi, water and earth) is emphasized, and the main treatment direction is to balance body fluids and remove black bile. Black grass seed oil can enhance immunity, reduce inflammation and nourish hair follicles. Terminalia chebula Retz. can regulate the balance of body fluids. Schizonepeta tenuifolia Briq. can antibacterial, anti - inflammatory and promote blood circulation. Black sesame has a nourishing effect, and the oil contained in sesame can deeply nourish and promote blood circulation. The essential oil can harmonize the medicinal properties, bring a pleasant mood and has a certain blood - activating effect. Through the moisturizing and nourishing of black grass seed oil and black sesame, the moisture and metabolism of the scalp are maintained, and the conversion of nutrients is promoted. The plant essential oil can relieve depression and anxiety, reduce the generation of black bile, and Terminalia chebula Retz. and Schizonepeta tenuifolia Briq. can purify and discharge the toxins in the scalp, improve the scalp health, so as to achieve the effects of hair growth and hair nourishment.

[0013] Wusiman grass and cnidium fruit are the monarch drugs. Wusiman grass is the first choice for Polygonum multiflorum Thunb. to tonify the liver and kidney, benefit essence and blood, and blacken hair and beard. It directly aims at the fundamental reason of "hair is the surplus of blood, and the luster of the kidney is manifested in the hair". It has extremely strong effects of opening blockages, drying dampness and astringing fluid, removing abnormal black bile and phlegm, strongly stimulating hair follicles, and promoting local blood circulation. It is responsible for nourishing the root of hair follicles. Cnidium fruit has the effects of warming the kidney and strengthening yang, drying dampness and expelling wind, killing insects and relieving itching. In the hair - growth formula, it is often used to improve the scalp environment, dispel wind - evil and dampness, and inhibit the problems of scalp itching and dandruff caused by Malassezia spp., creating a healthy environment for hair growth. Together with Wusiman grass, one tonifying and one clearing, they are the core together.

[0014] Walnut shell vinegar solution and chrysanthemum stem and leaf are the ministerial drugs. The vinegar solution obtained by pyrolysis of walnut shell has the effects of softening cutin, promoting blood circulation and removing stasis, and antibacterial, which can assist cnidium fruit to clean the scalp and dredge hair follicle openings. As a ministerial drug, the stem and leaf of chrysanthemum have the effect of clearing heat and dispelling wind. Used on the scalp, it can clear heat and reduce inflammation, soothe the swelling, redness, itching and pain of the scalp caused by wind - heat, and assist the monarch drug as a ministerial drug.

[0015] Chinese prickly ash is used as an adjuvant drug. Chinese prickly ash is pungent and warm in nature, and has the effects of warming the middle - jiao to stop pain, killing insects and relieving itching. It can promote blood circulation and remove stasis, dredge meridians, stimulate local blood circulation, and promote hair follicles to obtain nutrients. At the same time, its effect of killing insects and relieving itching can assist cnidium fruit. Because of its warm nature, it may be too stimulating to people with hot body constitution, so it is used as an adjuvant drug.

[0016] Aloe vera, used as an adjuvant, has heat-clearing, moisturizing, repairing, and hydrating effects, harmonizing the effects of other herbs. Its gel-like matrix helps to mitigate the irritation of herbs like Sichuan pepper, while simultaneously providing hydration and repair to the scalp. More importantly, its natural penetration-enhancing ability allows it to act as a "guide," leading to better absorption of other active ingredients by the skin. Sichuan pepper oil and chrysanthemum stem and leaf essential oil are extracted from Sichuan pepper and chrysanthemum stems and leaves, with aloe vera promoting the penetration of these essential oils. The volatile oils in Sichuan pepper (such as linalool and sapindus mucilage) have strong blood-activating and stimulating effects, significantly increasing blood circulation in scalp capillaries and the activity of hair papilla cells, delivering more nutrients to hair follicles. It also has a good inhibitory effect on common scalp fungi and bacteria. The active ingredients in chrysanthemum have excellent anti-inflammatory and antioxidant properties, which can soothe scalp inflammation, reduce the immune response around hair follicles (a significant factor in androgenetic alopecia), and create a peaceful environment for hair growth.

[0017] In addition, polysaccharides and anthraquinone compounds in aloe vera gel have been shown to effectively enhance the transdermal absorption rate of drugs. In synergy with essential oils, they can create a strong penetration-enhancing effect. Sichuan pepper essential oil is highly irritating and may cause discomfort if used directly. The cooling, moisturizing, and repairing properties of aloe vera can perfectly neutralize this irritation, improving the safety and comfort of the formula. Aloe vera gel is an excellent natural gel matrix that can serve as a carrier for essential oils, making them easy to apply and absorb.

[0018] Optionally, the hair loss prevention and hair growth herbal composition comprises the following raw materials in parts by weight:

[0019] 20-25 parts walnut shell vinegar, 20-25 parts black vine, 13-18 parts Sichuan pepper, 12-16 parts chrysanthemum stems and leaves, 12-18 parts aloe vera, 12-16 parts Cnidium monnieri, 6-8 parts Terminalia chebula, 13-17 parts black sesame, 6-8 parts black grass seed oil, 7-8 parts fermented rice water from mutton fat rice, 2-4 parts catnip, 2-4 parts plant essential oils.

[0020] By adopting the above technical solutions, the dosage of each raw material in the traditional Chinese medicine composition can be further refined, thereby improving its transdermal penetration rate and enhancing its effects on hair growth, hair loss prevention, and hair care.

[0021] Optionally, the plant essential oil is selected from at least one of rosemary essential oil, tea tree essential oil, peppermint essential oil, lavender essential oil, cedarwood essential oil, and rose essential oil.

[0022] By adopting the above technical solutions, rosemary essential oil has anti-inflammatory and circulation-improving effects. It contains monoterpenes, which can disrupt the intercellular lipid arrangement, increase skin permeability, and is highly lipid-soluble, allowing it to act as a carrier to carry other water-soluble active ingredients through the skin. The main active ingredient in tea tree essential oil is tea tree extract, which can improve the stratum corneum barrier structure, reduce skin resistance, and promote the penetration of active ingredients. Its small molecular structure can penetrate the scalp surface and enhance the penetration efficiency of other essential oils. It also has antibacterial, oil-controlling, and soothing effects. Peppermint essential oil contains menthol, which can locally stimulate and dilate scalp microvessels, indirectly improving the delivery efficiency of active ingredients to hair follicles, and also has an invigorating and oil-controlling effect.

[0023] Optionally, the fermented rice water from mutton fat rice is prepared by mixing mutton fat rice with water, grinding and filtering, mixing the resulting filtrate with compound bacteria, fermenting and sterilizing, and inoculating the compound bacteria at a rate of 0.05-0.1% of the filtrate mass. The compound bacteria contain Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis in a mass ratio of 5:1-2:0.5-1.

[0024] By adopting the above technical solution, mutton fat rice is a type of rice with high nutritional value. Its filtrate is rich in amino acids and small molecule peptides, which can provide direct nutrition to hair follicles. It also contains B vitamins, minerals, etc., which can maintain hair health, reduce hair loss, participate in protein synthesis, and are beneficial to hair follicle growth. Moreover, by mixing the filtrate with compound bacteria for fermentation, the microorganisms break down the large molecule proteins and polysaccharides in mutton fat rice into small molecule peptides, amino acids, oligosaccharides, etc., which are more easily absorbed by the scalp and hair follicles. This results in a stronger ability to penetrate the stratum corneum and higher bioavailability. In addition, the fermentation process produces a variety of vitamins, organic acids (such as lactic acid and acetic acid), antimicrobial peptides, etc., which can regulate the pH value, maintain a healthy weakly acidic environment, inhibit the excessive proliferation of harmful bacteria, and reduce dandruff and folliculitis. Therefore, the fermented mutton fat rice filtrate is a complex rich in highly bioactive small molecule nutrients, organic acids, and prebiotics. It achieves the purpose of nourishing hair and assisting hair growth through multiple pathways such as nourishing hair follicles, regulating scalp microecology, and anti-inflammatory soothing.

[0025] Optionally, the specific method for preparing the fermented rice water from mutton fat rice is as follows:

[0026] Mix mutton fat rice and water in a ratio of 1:5-10, grind and filter to obtain filtrate;

[0027] Add brewer's yeast and Bacillus subtilis to the filtrate, ferment at 30-37℃ for 12-24 hours, then add Lactobacillus plantarum, seal and ferment for 12-24 hours, centrifuge, and sterilize at 60-70℃ for 15-30 minutes.

[0028] By adopting the above technical solution, Bacillus subtilis and Saccharomyces cerevisiae are first used for fermentation. Bacillus subtilis can grow rapidly and produce proteases and amylases with strong acid-producing properties, which break down large protein molecules and starch into small peptides, amino acids and sugars, preparing fermentation raw materials for other strains. However, excessive production will produce acidic off-flavors and excessively consume nutrients, thus inhibiting the fermentation of other bacteria. Saccharomyces cerevisiae uses sugars to produce alcohol, carbon dioxide and abundant B vitamins and amino acids, which can provide prerequisite substances and nutrients for subsequent lactic acid fermentation. Lactobacillus plantarum is the main force and colonizer, which can use sugars to produce a large amount of lactic acid, rapidly reduce the pH value, complete the fermentation and inhibit other bacteria.

[0029] Optionally, the mutton fat rice is grown using water from the Yurungkash River.

[0030] By adopting the above-mentioned technical solutions, due to the advantages of water source and soil, its nutritional composition is more prominent than that of ordinary rice. Benefiting from the mineralization effect of glacial water, its mineral content is high: the content of trace elements such as calcium (30-50 mg / kg), iron (5-8 mg / kg), and zinc (2-3 mg / kg) is 1.5-2 times that of ordinary rice. The Yulongkash River's water source is glacial meltwater from the Kunlun Mountains, which is pure (free from industrial pollution) and highly mineralized (containing trace elements such as calcium, magnesium, potassium, and iron). During rice growth, the roots absorb minerals from the water, which are ultimately concentrated in the rice grains.

[0031] Secondly, this application provides a method for preparing a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, using the following technical solution:

[0032] A method for preparing a traditional Chinese medicine composition for preventing hair loss and promoting hair growth includes the following steps:

[0033] Includes the following steps:

[0034] Cnidium monnieri, Sichuan pepper, and chrysanthemum leaves and stems were extracted separately by steam extraction to obtain Cnidium monnieri oil, Sichuan pepper oil, and chrysanthemum leaves and stems oil.

[0035] Aloe vera is washed, dried, cut into pieces, pressed, and filtered to obtain aloe vera extract.

[0036] Black sesame seeds are crushed, and the black sesame powder is sealed and swollen with ethanol solution. The mixture is then filtered and concentrated until the solid content is ≥30% to obtain black sesame extract.

[0037] Mix Terminalia chebula, Smilax china and Schizonepeta tenuifolia, extract the juice, concentrate and sterilize it, add yeast, adjust the pH to 4.5-5.5, seal and ferment for 1-2 months, filter to obtain fermentation liquid;

[0038] A blended oil is prepared by mixing Sichuan pepper oil, chrysanthemum stem and leaf oil and Cnidium monnieri oil, adding black grass seed oil and plant essential oils;

[0039] The fermentation liquid was mixed evenly with walnut shell vinegar, aloe vera extract and rice water fermentation liquid of mutton fat rice to obtain a blended liquid;

[0040] The blended oil was added to the blended liquid, homogenized, and then black sesame extract was added and ultrasonically dispersed to obtain a traditional Chinese medicine composition for preventing hair loss and promoting hair growth.

[0041] By adopting the above technical solutions, the main active ingredients in Cnidium monnieri, Sichuan pepper, and chrysanthemum stems and leaves are volatile oils. Steam extraction is highly efficient, low-cost, requires no organic solvents, and produces pure and safe products, better preserving their natural properties and bioactivity. Aloe vera's main components are polysaccharides and anthraquinone compounds, which are heat-sensitive. Therefore, physical methods such as washing, cutting, and pressing are used to avoid high-temperature heating, preventing the damage and degradation of heat-sensitive components, and maximizing the preservation of aloe vera's natural activity and freshness. Ethanol swelling is used to efficiently extract fat-soluble components from black sesame seeds, which are then concentrated and dried for subsequent use. Bio-fermentation technology is used to ferment Terminalia chebula, Smilax china, and Nepeta cataria. During fermentation, large molecules are broken down into smaller molecules that are more easily absorbed by the skin and hair follicles. Furthermore, fermentation metabolism produces new and excellent components, such as vitamins, enzymes, and antibacterial substances, enhancing the nutritional and functional properties of the composition. Fermentation also reduces the irritation of the medicinal components, making them milder.

[0042] By mixing extracted essential oils with black sesame seed oil, a complementary balance of volatile and non-volatile oils is achieved, containing both highly active essential oil components and nourishing scalp oils. A nutrient-rich, pH-balanced water-based environment is created by mixing fermented liquid with walnut shell vinegar (which has astringent properties), aloe vera extract (moisturizing and soothing), and fermented rice water from mutton fat seeds (rich in vitamin B and amino acids). The blended oils and liquids are then homogenized to form a relatively stable emulsion system, preventing oil-water separation and ensuring a uniform product texture. Ultrasonic waves are used to disperse the black sesame extract into extremely fine particles throughout the system, greatly improving the powder's solubility and stability, preventing sedimentation, and promoting the release and absorption of active ingredients.

[0043] Optionally, the black sesame extract undergoes the following pretreatment:

[0044] Polylysine is added to water to prepare a polylysine solution with a concentration of 4-5 wt%, and glacial acetic acid is prepared into an acetic acid solution with a concentration of 1-1.5%.

[0045] Mix black sesame extract, polylysine solution and acetic acid solution in a mass ratio of 1:2.5-3:1.5-2, stir evenly, adjust pH to 4.5-5, sonicate for 10 minutes, and concentrate to ≥30% solids.

[0046] By adopting the above technical solution, black sesame extract contains active ingredients such as oils and polyphenols, most of which are hydrophobic or weakly polar substances. The barrier function of the stratum corneum is hindered by the percutaneous penetration of the skin. The stratum corneum is composed of keratinocytes and intercellular lipids. The hydrophobic lipid matrix will hinder the penetration of hydrophilic components, while large molecules or polar components are difficult to break through the tight action of keratinocytes. Acetic acid (an amino acid analog that can act as a hydrogen bond donor) and polylysine (a cationic polypeptide that can act as a hydrogen bond acceptor) can regulate the skin barrier and promote penetration through chemical action. The carboxyl group of acetic acid can provide protons, acting as a hydrogen bond donor. Mechanisms that improve permeability include disrupting the hydrogen bond network of intercellular lipids in the stratum corneum, altering the hydrophilicity of keratinocytes, regulating the pH of the system, and enhancing the solubility of the extract. Meanwhile, the amino group of polylysine can accept protons, acting as a hydrogen bond acceptor. It synergistically interacts with acetic acid, forming hydrogen bond bridges with it, enhancing permeability stability, prolonging retention time in the stratum corneum, and continuously disrupting the lipid barrier. It can also form dual interactions with the stratum corneum—hydrogen bonding and electrostatic interaction—acting as a carrier to pull the hydrophobic / polar components of black sesame extract to the stratum corneum. Internally, it reduces the loss of ingredients from the skin, and the synergistic effect of polylysine also includes increasing the hydrophilicity of the system and promoting the penetration of water-soluble ingredients. The components of black sesame extract can complement the effects of acetic acid and polylysine. Its hydrophobic components, such as sesamin and sterols, are soluble in the hydrophobic region of the acetic acid-polylysine complex and penetrate into the lipid matrix of the stratum corneum with the complex. Moreover, the polar components in the extract, such as phenolic acids and small molecule peptides, can quickly pass through the loose keratinocyte channels through the hydrogen bonding between acetic acid and the stratum corneum and the electrostatic attraction of polylysine, avoiding being blocked by the skin barrier.

[0047] Optionally, the aloe vera extract is pretreated with a hyaluronic acid derivative. The pretreatment method is as follows: the aloe vera extract is dispersed in an ethanol solution to obtain an aloe vera dispersion, the hyaluronic acid derivative is added to the ethanol solution, the aloe vera dispersion is added, the mixture is stirred at room temperature, the ethanol is evaporated by rotary evaporation, and the mixture is concentrated. The mass ratio of the hyaluronic acid derivative to the aloe vera extract is 5:3.

[0048] By adopting the above technical solution, the polarity of the aloe dispersion added to ethanol is reduced, creating favorable conditions for the dissolution and subsequent self-assembly of hyaluronic acid derivatives. When hyaluronic acid derivatives are mixed with aloe dispersion in ethanol, the derivative molecules expand, the polarity of the system is enhanced, and the transparent derivative molecules begin to aggregate and self-assemble. During the self-assembly process, hydrophobic active ingredients in aloe vera extract, such as aloe-emodin and sterols, are captured into the core of the derivative, while hydrophilic components, such as aloe polysaccharides, can combine with the hydrophilic hyaluronic acid shell through hydrogen bonding or be physically encapsulated in the fence layer of micelles, thereby forming a nanocarrier structure. The resulting micelles or nanoparticles are extremely small in size. Ions of this size can pass through the intercellular spaces of the stratum corneum of the skin and are more likely to attach and enter the hair follicle opening for deep delivery. The carrier shell made of hyaluronic acid can better target and enrich these nano-ions around the cells, improving drug utilization. Encapsulating unstable aloe vera active ingredients inside the nanocarrier can protect them from degradation and inactivation during storage and scalp treatment, ensuring that sufficient activity is available before reaching the site of action.

[0049] Thirdly, this application provides an application of a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, using the following technical solution:

[0050] An application of a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, applied to a shampoo, wherein the shampoo contains the following raw materials in parts by weight: 1-60 parts of the traditional Chinese medicine composition for preventing hair loss and promoting hair growth, 1-4 parts of poloxamer 101, 0.1-5 parts of thickener, 1-5 parts of conditioning agent, 5-40 parts of surfactant, 0.5-1 part of preservative, and 5-80 parts of solvent.

[0051] By adopting the above technical solution, not only is a traditional Chinese medicine composition added to the shampoo, but also a certain amount of poloxamer 101 is used. Poloxamer is an amphiphilic polyoxyethylene-polyoxypropylene copolymer that can improve the solubility, stability, release and bioavailability of drugs. It has excellent safety, excellent wetting and solubilizing ability. After contact with the skin, it can produce a good penetration-promoting effect and improve the hair growth effect of the traditional Chinese medicine composition.

[0052] Optionally, the surfactant is selected from at least one of ammonium lauryl sulfate, sodium lauryl ether sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, cocoyl diethanolamine, and sodium lauryl ether sulfate.

[0053] The thickener is selected from at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, carbomer, gum arabic, and sodium alginate;

[0054] The conditioning agent is selected from at least one of guar gum, polyquaternium salt, polydimethylsiloxane, decamethylcyclopentasiloxane, guar gum hydroxypropyltrimethylammonium chloride, and polydimethylsiloxane alcohol;

[0055] The preservative is selected from at least one of hydroxybenzoic acid esters, methylisothiazolinone, imidazolidinyl urea, phenoxyethanol, and sodium benzoate;

[0056] The solvent is selected from at least one of ethanol, glycerol, propylene glycol, and water.

[0057] In summary, this application has the following beneficial effects:

[0058] 1. This application uses *Ulmus pumila* and *Cnidium monnieri* as principal herbs, which can stimulate hair follicles, promote local blood circulation, nourish hair follicles, and improve the scalp environment. Walnut shell vinegar and chrysanthemum leaf stems are assistant herbs, which can invigorate blood circulation, remove blood stasis, unclog hair follicles, clear heat and reduce inflammation, and soothe redness, swelling, itching, and pain. Sichuan pepper is used as an adjuvant herb, which can unclog meridians and promote the absorption of nutrients by hair follicles. Aloe vera is used as a guide herb, which can alleviate the irritation of Sichuan pepper, provide moisturizing and repairing effects. Moreover, the natural penetration-enhancing ability of aloe vera can promote the penetration effect of other ingredients, improve the effects of preventing hair loss and promoting hair growth. In addition, it is combined with the mechanism of Uyghur medicine, with black jujube, chebula, schizonepeta, and black sesame working together to achieve the effects of balancing body fluids, antibacterial, nourishing, and moisturizing, improving scalp health, preventing hair loss, and increasing new hair growth.

[0059] 2. The method of this application employs the most suitable extraction method based on the physicochemical properties of different raw materials, ensuring the full acquisition of core effective components. Furthermore, through fermentation, ultrasonic dispersion, and other technologies, macromolecules are transformed into small molecules, forming a uniform and stable system, which greatly improves the absorption efficiency of nutrients by hair follicles. Multiple traditional Chinese medicine ingredients are compounded in various forms such as oil phase, aqueous phase, and fermentation broth to produce synergistic effects, working together to promote scalp health through multiple pathways such as oil control, anti-inflammation, blood circulation promotion, and nutrition. In addition, the use of physical methods and biological fermentation avoids the use of chemical solvents and high-temperature treatment, making the product closer to nature, less irritating to the scalp, and conducive to maintaining the scalp's microecological balance.

[0060] 3. In this application, acetic acid and polylysine are preferably used to pretreat black sesame extract. Through the functions of hydrogen bond donor and hydrogen bond acceptor, the two can soften the stratum corneum barrier, improve the permeability of black sesame extract, penetrate the scalp barrier more efficiently, reach the hair follicle, and obtain a more significant anti-hair loss and hair growth effect.

[0061] 4. In this application, hyaluronic acid derivatives are preferably used to pretreat aloe vera extract, thereby encapsulating the active ingredients in the aloe vera extract and constructing the effective ingredients in the aloe vera extract into nanoparticles with targeting and penetration-enhancing capabilities. This greatly enhances the soothing, anti-inflammatory, and moisturizing effects of aloe vera, and works synergistically with other ingredients to act on hair follicles and improve the overall hair growth effect. Detailed Implementation

[0063] The following embodiments provide a further detailed description of this application.

[0064] Preparation of fermented rice water from mutton fat rice (Example 1-2)

[0065] In the following preparation examples, *Lactobacillus plantarum* was selected from Qingdao Haibo Biotechnology Co., Ltd., catalog number HBJZ066; *Saccharomyces cerevisiae* was selected from Beijing Yuwei Technology Co., Ltd., catalog number CICC30290; and *Bacillus subtilis* was selected from Qingdao Haibo Biotechnology Co., Ltd., catalog number HBJZ018.

[0066] Preparation Example 1: Mix mutton fat rice with 5 times its weight of water, grind and filter to obtain filtrate. The mutton fat rice is obtained by irrigating with Yulongkash River water.

[0067] Saccharomyces cerevisiae and Bacillus subtilis were added to the filtrate, and fermented at 37°C for 12 hours. Then, Lactobacillus plantarum was added, and the mixture was sealed and fermented for another 12 hours. After centrifugation, the mixture was sterilized at 70°C for 15 minutes. The total inoculum of Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis was 0.1% of the filtrate mass. The filtrate was prepared by mixing rice fat with water, grinding and filtering, and then mixing the resulting filtrate with the compound bacteria before fermentation and sterilization. The mass ratio of the mixture was 5:2:1.

[0068] Preparation Example 2: Mix mutton fat rice with 10 times its weight of water, grind and filter to obtain filtrate. The mutton fat rice is obtained by irrigating with Yulongkash River water.

[0069] Saccharomyces cerevisiae and Bacillus subtilis were added to the filtrate, and fermented at 30°C for 24 hours. Then, Lactobacillus plantarum was added, and the mixture was sealed and fermented for another 24 hours. After centrifugation, the mixture was sterilized at 60°C for 30 minutes. The total inoculum of Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis was 0.05% of the filtrate mass. The filtrate was prepared by mixing rice fat with water, grinding and filtering, and then mixing the resulting filtrate with the compound bacteria before fermentation and sterilization. The mass ratio of the mixture was 5:1:0.5.

[0070] Preparation examples of hyaluronic acid derivatives

[0071] In the preparation example, the hydrogen-form cation exchange resin was selected from Langfang Dingyuan Chemical Building Materials, model 001×7 (732).

[0072] Preparation Example 3: Dissolve 1g of hyaluronic acid in 100mL of pure water, sonicate for 5min to fully dissolve the hyaluronic acid, add 3g of hydrogen-form cation exchange resin, stir at room temperature for 5h, filter to obtain filtrate, adjust the pH of the filtrate to 7 with tetrabutylammonium hydroxide, freeze dry to obtain pretreated hyaluronic acid.

[0073] Valproic acid, N,N-dicyclohexylcarbodiimide, and 4-dimethylaminopyridine were dissolved in 20 ml of anhydrous dimethyl sulfoxide and reacted at 60 °C for 1 h. Then, pretreated hyaluronic acid was added and reacted at 60 °C for 2 days. The mixture was then dissolved in 0.01 mg / ml sodium chloride and dialyzed for 24 h to remove hyaluronic acid from the system. The mixture was then dialyzed with pure water for 48 h and freeze-dried. The molar ratio of hyaluronic acid to valproic acid was 5:1, and the molar ratio of valproic acid to N,N-dicyclohexylcarbodiimide and 4-dimethylaminopyridine was 1:1:1.

[0074] Example

[0075] In the following examples, black grass seed oil was selected from Jiangxi Yuanshangcao Linqin, with the product number YSC116; lavender essential oil was selected from Shanghai Yuanye Biotechnology, with the product number S25516; yeast was selected from Hubei Chengfeng Chemical, with the product number 001; and polylysine was selected from Beijing Bio-Lybo Technology, with the product number SY0521-LHK.

[0076] Example 1: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, comprising the following raw materials by weight: 25g walnut shell vinegar, 25g black sesame seed oil, 18g Sichuan pepper, 16g chrysanthemum stems and leaves, 18g aloe vera, 16g Cnidium monnieri, 8g Terminalia chebula, 17g black sesame seeds, 8g black sesame seed oil, 8g fermented rice water from mutton fat rice, 4g Schizonepeta tenuifolia, and 4g plant essential oil; wherein the walnut shell vinegar is prepared by crushing walnut shells, pressing them into rods, carbonizing them at 400℃ for 3 hours, cooling the flue gas to obtain liquid, adding 2 times the liquid weight of purified water and 0.1 times the liquid weight of ginger powder, sealing and storing for 3 months, filtering and separating the pigments, the fermented rice water from mutton fat rice is prepared from Example 1, and the plant essential oil is lavender essential oil.

[0077] The preparation method of the above-mentioned traditional Chinese medicine composition for preventing hair loss and promoting hair growth includes the following steps:

[0078] S1. Cnidium monnieri, Sichuan pepper, and chrysanthemum stem and leaf extracts were obtained separately using steam extraction to produce Cnidium monnieri oil, Sichuan pepper oil, and chrysanthemum stem and leaf oil. The specific method was as follows: Cnidium monnieri was pulverized to 40 mesh, soaked in 1.5 times its weight of water for 2 hours, then heated to 100℃ and distilled for 4 hours. After condensation and settling, the oil layer was separated. 5% anhydrous sodium sulfate (by weight of the oil layer) was added, stirred, and allowed to stand for 4 hours. The mixture was then filtered through a 0.22μm organic filter membrane to obtain Cnidium monnieri oil. Sichuan pepper was washed, and water was added... The oil layer was heated to 100℃ and kept at that temperature for 5 hours. When the oil layer stopped increasing, the heating was stopped, the mixture was condensed, and allowed to stand for 12 hours. The oil layer was collected, and 2% of the oil layer mass of activated carbon was added to it. The mixture was stirred in a water bath at 40℃ for 30 minutes, allowed to stand for 2 hours, and then filtered to obtain Sichuan pepper oil. Fresh chrysanthemum stems and leaves were washed, drained, heated to 95℃, kept at that temperature for 3 hours, condensed, allowed to stand for 12 hours, and the oil layer was separated. Anhydrous magnesium sulfate (5% of the oil layer mass) was added, and the mixture was filtered to obtain chrysanthemum stem and leaf oil.

[0079] S2. Wash, dry, cut, press, filter, and concentrate the aloe vera to a solid content of 70% to obtain aloe vera extract.

[0080] S3. Grind black sesame seeds into 100 mesh, seal and swell them in a 65% ethanol solution for 24 hours, filter and concentrate until the solid content is 50% to obtain black sesame extract.

[0081] S4. Wash and drain the water from the *Usnea indica* and *Nepeta cataria*, mix them with *Terminalia chebula*, extract the juice, concentrate it to 60% solids, add 0.05% yeast by weight of the concentrate, adjust the pH to 5.5 with acetic acid solution, seal and ferment for 2 months, filter to obtain fermented liquid;

[0082] S5. Mix Sichuan pepper oil, chrysanthemum stem and leaf oil and Cnidium monnieri oil, add black grass seed oil and plant essential oil, mix well to obtain blended oil;

[0083] S6. Mix the fermentation liquid with walnut shell vinegar, aloe vera extract and rice water fermentation liquid to obtain a blended liquid.

[0084] S7. Add the blended oil to the blended liquid, homogenize for 5 minutes, then add black sesame extract, and ultrasonically disperse for 30 minutes to obtain the hair loss prevention and hair growth traditional Chinese medicine composition.

[0085] Example 2: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, comprising the following raw materials by weight: 20g walnut shell vinegar, 20g black sesame seeds, 13g Sichuan pepper, 12g chrysanthemum stems and leaves, 12g aloe vera, 12g Cnidium monnieri, 6g Terminalia chebula, 13g black sesame seeds, 6g black sesame seed oil, 7g fermented rice water from mutton fat rice, 2g Schizonepeta tenuifolia, and 2g plant essential oil; wherein the walnut shell vinegar is prepared by crushing walnut shells, pressing them into rods, carbonizing them at 400℃ for 3 hours, cooling the flue gas to obtain liquid, adding 2 times the liquid weight of purified water and 0.1 times the liquid weight of ginger powder, sealing and storing for 3 months, filtering and separating the pigments, the fermented rice water from mutton fat rice is prepared as in Example 2, and the plant essential oil is lavender essential oil.

[0086] The preparation method of the above-mentioned traditional Chinese medicine composition for preventing hair loss and promoting hair growth includes the following steps:

[0087] S1. Cnidium monnieri, Sichuan pepper, and chrysanthemum leaves and stems are extracted separately by steam extraction to obtain Cnidium monnieri oil, Sichuan pepper oil, and chrysanthemum stem and leaf oil; the specific method is the same as in Example 1.

[0088] S2. Wash, dry, cut, press, filter, and concentrate the aloe vera to a solid content of 70% to obtain aloe vera extract.

[0089] S3. Grind black sesame seeds into 100 mesh, seal and swell them in a 65% ethanol solution for 24 hours, filter and concentrate until the solid content is 50% to obtain black sesame extract.

[0090] S4. Wash and drain the water from the *Usnea indica* and *Nepeta cataria*, mix them with *Terminalia chebula*, extract the juice, concentrate it to 70% solids, add 0.05% yeast by weight of the concentrate, adjust the pH to 4.5 with acetic acid solution, seal and ferment for 1 month, filter to obtain fermented liquid.

[0091] S5. Mix Sichuan pepper oil, chrysanthemum stem and leaf oil and Cnidium monnieri oil, add black grass seed oil and plant essential oil, mix well to obtain blended oil;

[0092] S6. Mix the fermentation liquid with walnut shell vinegar, aloe vera extract and rice water fermentation liquid to obtain a blended liquid.

[0093] S7. Add the blended oil to the blended liquid, homogenize for 3 minutes, then add black sesame extract, and ultrasonically disperse for 20 minutes to obtain the hair loss prevention and hair growth traditional Chinese medicine composition.

[0094] Example 3: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, differing from Example 1 in the following proportions of raw materials: 30g walnut shell vinegar, 30g black sesame seed oil, 20g Sichuan pepper, 20g chrysanthemum stems and leaves, 20g aloe vera, 20g Cnidium monnieri, 10g Terminalia chebula, 20g black sesame seeds, 10g black sesame seed oil, 10g fermented rice water from mutton fat rice, 5g Schizonepeta tenuifolia, and 5g plant essential oil; wherein the walnut shell vinegar is prepared by crushing walnut shells, pressing them into rods, carbonizing them at 400℃ for 3 hours, cooling the flue gas to obtain liquid, adding 2 times the liquid mass of purified water and 0.1 times the liquid mass of ginger powder, sealing and storing for 3 months, filtering and separating the pigments, the fermented rice water from mutton fat rice is prepared as in Example 1, and the plant essential oil is lavender essential oil.

[0095] Example 4: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, differing from Example 1 in the following proportions of raw materials: 100g walnut shell vinegar, 10g black sesame seeds, 10g Sichuan pepper, 10g chrysanthemum stems and leaves, 10g aloe vera, 10g Cnidium monnieri, 5g Terminalia chebula, 10g black sesame seeds, 5g black sesame seed oil, 5g fermented rice water from mutton fat rice, 1g Schizonepeta tenuifolia, and 1g plant essential oil; wherein the walnut shell vinegar is prepared by crushing walnut shells, pressing them into rods, carbonizing them at 400℃ for 3 hours, cooling the flue gas to obtain liquid, adding 2 times the liquid mass of purified water and 0.1 times the liquid mass of ginger powder, sealing and storing for 3 months, filtering and separating the pigments, the fermented rice water from mutton fat rice is prepared as in Example 1, and the plant essential oil is lavender essential oil.

[0096] Example 5: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, differing from Example 1 in that the black sesame extract is pretreated before being added to the blending liquid and the mixture of the blending liquids. The pretreatment method is as follows:

[0097] Polylysine was added to water to prepare a polylysine emulsion with a concentration of 5 wt%, and glacial acetic acid was diluted with water to prepare an acetic acid solution with a concentration of 1.5%.

[0098] The black sesame extract, polylysine solution, and acetic acid solution were mixed in a mass ratio of 1:3:2. After stirring evenly, the pH was adjusted to 5, and the mixture was sonicated for 10 minutes to concentrate it to 50% solids.

[0099] Example 6: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, differing from Example 1 in that the black sesame extract is pretreated before being added to the blending liquid and the mixture of the blending liquids. The pretreatment method is as follows:

[0100] Polylysine was added to water to prepare a polylysine emulsion with a concentration of 4 wt%, and glacial acetic acid was diluted with water to prepare an acetic acid solution with a concentration of 1.0%.

[0101] The black sesame extract, polylysine solution, and acetic acid solution were mixed in a mass ratio of 1:2.5:1.5. After stirring evenly, the pH was adjusted to 4.5, and the mixture was sonicated for 10 minutes to concentrate it to 50% solids.

[0102] Example 7: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, differing from Example 5 in that the black sesame extract is pretreated before being added to the blending liquid and the mixture thereof. The pretreatment method is as follows:

[0103] Arginine was added to water to prepare an arginine emulsion with a concentration of 4 wt%, and glacial acetic acid was diluted with water to prepare an acetic acid solution with a concentration of 1.0%.

[0104] The black sesame extract, arginine solution, and acetic acid solution were mixed in a mass ratio of 1:2.5:1.5. After stirring evenly, the pH was adjusted to 4.5, and the mixture was sonicated for 10 minutes to concentrate it to 50% solids.

[0105] Example 8: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, differing from Example 5 in that the aloe vera extract is pretreated with hyaluronic acid derivatives before being mixed with fermentation broth, walnut shell vinegar, and fermentation broth made from rice water. The specific pretreatment method is as follows: the aloe vera extract is dispersed in twice its mass of 90% ethanol to obtain an aloe vera dispersion.

[0106] The hyaluronic acid derivative was added to a 90% ethanol solution at twice its mass. The aloe vera dispersion was slowly added while stirring at 1000 r / min. After stirring at room temperature for 30 min, the ethanol was evaporated by rotary evaporation at 45℃ and the mixture was concentrated to a solid content of 70%. The mass ratio of the hyaluronic acid derivative to the aloe vera concentrate was 5:3.

[0107] Comparative Example

[0108] Comparative Example 1: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which differs from Example 1 in that it uses an equal amount of chrysanthemum stems and leaves instead of Cnidium monnieri, that is, the amount of chrysanthemum stems and leaves is 32g and the amount of Cnidium monnieri is 0g.

[0109] Comparative Example 2: A Chinese herbal composition for preventing hair loss and promoting hair growth, which differs from Example 1 in that an equal amount of Schizonepeta tenuifolia is used instead of Usnea ventricosa, i.e., the amount of Schizonepeta tenuifolia is 29g and the amount of Usnea ventricosa is 0g.

[0110] Comparative Example 3: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which differs from Example 1 in that an equal amount of walnut shell vinegar is used to replace aloe vera extract, that is, the amount of walnut shell vinegar is 43g and the amount of aloe vera is 0g.

[0111] Comparative Example 4: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which differs from Example 1 in that it does not contain black sesame extract.

[0112] Comparative Example 5: A traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which differs from Example 1 in that it does not contain Terminalia chebula, Smilax china, and Schizonepeta tenuifolia, i.e., it does not contain fermentation liquid.

[0113] Application examples

[0114] Application Example 1: A shampoo containing a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, comprising the following raw materials by weight: 40g of the traditional Chinese medicine composition for preventing hair loss and promoting hair growth prepared in Example 1, 4g of poloxamer 101, 20g of detergent, 1g of thickener, 4g of conditioning agent, 8g of surfactant, 1g of preservative, and 70g of solvent, wherein the detergent is sodium dodecylbenzenesulfonate, the thickener is carbomer, the conditioning agent is polydimethylsiloxane, the surfactant is cocamidopropyl betaine, the preservative is phenoxyethanol, and the solvent is water; the preparation method of the above shampoo includes the following steps:

[0115] (1) Add the thickener, solvent, poloxamer 101, surfactant, and hair loss prevention and hair growth Chinese medicine composition to a homogenizer, heat to 75°C, and stir for 15 minutes;

[0116] (2) Add the cleaning agent and conditioning agent to the stainless steel bucket, heat until dissolved, and add the dissolved aerogel and conditioning agent when the homogenizer is cooled to 70°C. Stir evenly at a stirring speed of 20r / min.

[0117] (3) Add the surfactant when the homogenizing pot cools down to 50°C;

[0118] (4) When the homogenizing pot cools down to 40°C, add the preservative, stir well, and vacuum for 10 minutes;

[0119] (5) After the homogenizing pot cools down to 35°C, discharge the material, seal it, and let it stand for 36 hours before filling.

[0120] Application Examples 2-8: A shampoo containing a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which differs from Application Example 1 in that the traditional Chinese medicine composition for preventing hair loss and promoting hair growth is prepared according to Examples 2-8.

[0121] Application Examples 9-13: A shampoo containing a traditional Chinese medicine composition for preventing hair loss and promoting hair growth, which differs from Application Example 1 in that the traditional Chinese medicine composition for preventing hair loss and promoting hair growth is made from Comparative Examples 1-5.

[0122] Performance testing

[0123] 1. Testing of the hair loss prevention and hair growth traditional Chinese medicine composition: The hair loss prevention and hair growth traditional Chinese medicine composition was prepared according to the methods in Examples 1-8 and Comparative Examples 1-5, and its performance was tested according to the following methods. The test results are recorded in Table 1.

[0124] 1. Detection of 5α-reductase inhibition rate

[0125] Principle: 5α-Reductase is a protease that stimulates the conversion of testosterone to dihydrotestosterone (DHT). When DHT accumulates to high levels in the skin, it causes androgenetic alopecia. The pathogenesis involves DHT inhibiting hair follicle growth and development, leading to poor follicle development, atrophy, and decreased hair quality. Furthermore, elevated DHT levels cause sebaceous gland hypertrophy and increased sebum secretion, further affecting the hair follicle's growth environment. Therefore, the higher the 5α-reductase level, the more severe the hair loss.

[0126] (1) Materials and methods: 5α-reductase; prostate tissue extracted from Mr. Zi was stored at -80℃; negative control group: finasteride (0.4μM); blank group: phosphate buffer (PBS); others: testosterone, reductase II, chloroform;

[0127] (2) Sample preparation: Take 1g of the hair loss prevention and hair growth herbal composition sample, add 2ml of water to dissolve it, and dilute it with water to a concentration of 1%;

[0128] (3) Experimental procedure: The 2ml reaction system contained: 250μL phosphate buffer (pH 6.5); 700μL enzyme extract (1mg / mL); 100μL testosterone solution (0.5mg / mL); and 350μL NADPH solution (1mg / mL).

[0129] Add 600 μL of 0.4 μM finasteride to the positive control tube;

[0130] Add 600 μL of sample solution to the sample tube;

[0131] Add 600 μL of water to the complete reaction tube;

[0132] The control tube was modified by replacing the enzyme extract with phosphate buffer (pH 6.5) and adding 2.0 mL of dichloromethane to terminate the reaction.

[0133] The sample tubes were reacted at 37℃ for 30 min. After the reaction was completed, 2.0 mL of dichloromethane was added to stop the reaction. After shaking for 1 min, the tubes were centrifuged at 3000 r / min for 10 min to remove the upper aqueous phase. About 1.0 mL of organic phase was removed and evaporated to dryness. The residue was dissolved in 1.0 mL of methanol. The peak area (T) of testosterone was determined by high performance liquid chromatography. Three parallel tubes were used for each reaction. The average value was taken and retained to one decimal place. The calculation formula is as follows: Inhibition rate (100%) = [(T sample tube - T reaction tube) / (T control tube - T reaction tube) × 100].

[0134] 2. Antibacterial rate: Pre-activated Malassezia furfur was prepared into a bacterial suspension with a concentration of 10⁶ CFU / mL using a culture medium (60g agar, 20g dried ox bile, 10g Tween 40, 2.5g glyceryl monostearate, 1000ml distilled water, sterilized at 121℃ for 15min). The hair loss prevention and hair growth herbal composition was prepared into a 15% (w / w) sample solution using sterile water. 0.5ml of the bacterial suspension was placed in a test tube, and 4.5ml of the sample solution was added. The mixture was quickly mixed and the timing was immediately started. 1.0ml of the mixture was taken at different time points, and the viable bacteria count was determined using a viable bacteria culture counting method. Each sample was inoculated into three petri dishes, and the antibacterial rate was calculated.

[0135] 3. Assay for promoting hair follicle cell proliferation in mice: Purebred C57BL / 6 mice weighing 15-20g (both male and female) were used. Under mild ether anesthesia, the hair on the backs of the mice was plucked using a mixture of rosin and paraffin, covering an area of ​​approximately 3cm × 4cm. The mice were then divided into two groups: the example group and the comparative group. The day after hair removal, the sample was evenly applied to the plucked area on the backs of each group of mice. The dosage for each experimental group was 10ml / kg, once daily, for 20 consecutive days. Afterward, the skin from the plucked area on the backs of the mice was collected, fixed with formalin, and used for pathological sectioning to observe hair follicle growth. The number of hair follicles was counted from 10 mice for each sample. The length of newly grown hair in the plucked area was measured on the second day after 20 days of application. The measurement method involved plucking the 10 longest newly grown hairs from the plucked area using a magnifying glass and tweezers, and measuring the length with calipers.

[0136] Table 1

[0137]

[0138] Table 1 shows that the traditional Chinese medicine compositions prepared using different amounts of raw materials in Examples 1-4 have a high inhibition rate of 5α-reductase, fast antibacterial speed, good antibacterial effect, can promote hair follicle proliferation, accelerate hair growth, and achieve a good anti-hair loss effect.

[0139] In Examples 5 and 6, polylysine and glacial acetic acid were used to pretreat black sesame extract. It was observed that the inhibition rate of 5α-reductase did not change significantly, but the antibacterial speed was slightly accelerated. This indicates that polylysine and glacial acetic acid can assist the penetration of black sesame extract, accelerate diffusion, assist in antibacterial activity, and accelerate hair growth and promote hair follicle proliferation.

[0140] In Example 7, arginine and glacial acetic acid were used to pretreat the black sesame extract. It can be seen that compared with Example 5, its antibacterial rate and 5α-reductase inhibition rate did not change significantly.

[0141] Compared to Example 5, Example 8 further pretreated the aloe vera extract with hyaluronic acid derivatives. As can be seen, the antibacterial rate of the traditional Chinese medicine composition was significantly increased, indicating that the aloe vera penetration rate was further increased, thus improving hair follicle proliferation.

[0142] In Comparative Example 1, Chrysanthemum leaves and stems were used instead of Cnidium monnieri, and in Comparative Example 2, Nepeta cataria was used instead of Eupatorium fortunei. Compared with Example 1, it can be seen that the inhibition rate of 5α-reductase in Comparative Example 1 decreased and the antibacterial ability weakened. Although Nepeta cataria had a better antibacterial effect in Comparative Example 2, its inhibition rate of 5α-reductase decreased.

[0143] In Comparative Example 3, walnut shell vinegar was used instead of aloe vera extract. Compared with Example 1, although the inhibition rate of 5α-reductase did not change significantly, the antibacterial speed was slower, indicating that aloe vera extract can increase the penetration rate of the traditional Chinese medicine composition and accelerate the antibacterial effect.

[0144] In Comparative Example 4, no black sesame extract was added. Compared with Example 1, the inhibition rate of 5α-reductase decreased and the antibacterial rate was slightly reduced. It can be seen that black sesame extract can increase the inhibition rate of 5α-reductase and play an auxiliary antibacterial role.

[0145] In Comparative Example 5, no Terminalia chebula, Smilax china, or Schizonepeta tenuifolia were added. Compared with Example 1, the Chinese herbal composition prepared showed a significant decrease in the inhibition rate of 5α-reductase and a significant decrease in the antibacterial rate.

[0146] II. Performance testing of shampoos containing traditional Chinese medicine compositions: Prepare shampoos according to the method in the application examples, and conduct performance tests according to the following methods. Record the test results in Table 2.

[0147] Hair density: 140 male volunteers aged 25-45 years were selected from those suffering from hair loss. The inclusion criteria were: mild to moderate hair loss, hair loss grade Norwood-Hamilton II to III, or Ludwig I to II, no other diseases related to hair loss recently, and exclusion of (1) those with atrophied hair follicles; (2) those with serious systemic diseases, immunodeficiency or autoimmune diseases; (3) those with dermatological diseases in the test area or those who are receiving drug treatment, such as scalp infection, seborrheic dermatitis, psoriasis and alopecia areata; (4) those who have used topical or systemic hair growth products or used topical or systemic drugs that affect hair growth or loss acid production in the three months prior to the start of the test; (5) those who have received hair transplantation. Students were randomly divided into groups 1-14. Groups 1-13 used the shampoo prepared in Examples 1-13, while the control group received no treatment. Shampoo was performed every other day, massaging the scalp with the shampoo for at least 3 minutes during washing. Hair density was measured before and after shampooing using the following method: ① Test area selection: To ensure accuracy, a classic, uniform scalp area was selected (avoiding the hairline, balding areas, or areas with excessively dense hair). The most commonly selected area was the central area of ​​the top of the head: A circular area with a diameter of 2cm (approximately 3.14cm²) was drawn centered on the Baihui acupoint on the top of the head. 2 Or, take a square area 2cm behind the hairline on the forehead: 2cm wide and 2cm long (area 4cm²). 2 ); ② Operating steps: Clean the scalp, wash the hair with neutral shampoo and dry it to avoid oil and dandruff interfering with observation; Fix the hair: Gently comb the hair in the measurement area with a fine-tooth comb to ensure there are no knots or frizz; Microscopic observation: Use a dermatoscope (magnification 10-100x) to aim at the measurement area and take a high-resolution image; Count the hairs: Mark and count all visible hairs in the area on the image (Note: Under the dermatoscope, "terminal hairs" (coarse and stiff hairs) and "vellus hairs" (fine and soft lanugo) are visible. Usually, the number of terminal hairs is counted because vellus hairs do not affect density assessment); Calculate the density: Hair density (roots / cm²) 2 = Number of hairs in the measurement area / area of ​​the measurement area. For example, if 400 terminal hairs are counted in a circular area with a diameter of 2cm (area ≈ 3.14cm²), the density is: 400 / 3.14 ≈ 127 hairs / cm² (close to the normal range for Caucasians). Observe continuously for 5-10 weeks, and measure hair density weekly. The test results are taken as the average of 10 people in each group.

[0148] Table 2

[0149]

[0150] As can be seen from Table 1, the traditional Chinese medicine compositions prepared using different amounts of raw materials in Examples 1-4, when made into shampoos, have a good function of preventing hair loss and promoting hair regeneration when used continuously, and can effectively improve hair quality and quantity.

[0151] In Examples 5-6, pretreatment of black sesame extract with polylysine and glacial acetic acid effectively improved hair growth rate; however, in Example 7, the use of arginine resulted in a slight decrease in hair density and a reduced improvement effect.

[0152] Compared to Example 5, Example 8 still used hyaluronic acid derivatives to pretreat aloe vera extract, and the hair density was significantly increased.

[0153] However, the hair growth effect of the traditional Chinese medicine compositions prepared in Comparative Examples 1-5, when added to shampoo and used continuously, was not as good as that in Example 1.

[0154] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A Chinese medicine composition for preventing hair loss and promoting hair growth, characterized in that, The raw materials include 10-30 parts of walnut shell vinegar, 10-30 parts of u silk grass, 10-20 parts of pepper, 10-20 parts of chrysanthemum stem and leaf, 10-20 parts of aloe, 10-20 parts of cnidium, 5-10 parts of Terminalia chebula, 10-20 parts of black sesame, 5-10 parts of black grass seed oil, 5-10 parts of sheep fat seed rice washing water fermentation liquid, 1-5 parts of catmint, and 1-5 parts of plant essential oil.

2. The anti-shedding hair growth traditional Chinese medicine composition according to claim 1, characterized in that: The anti-hair loss and hair growth traditional Chinese medicine composition contains the following raw materials by weight: 20-25 parts of walnut shell vinegar, 20-25 parts of u silk grass, 13-18 parts of pepper, 12-16 parts of chrysanthemum stem and leaf, 12-18 parts of aloe, 12-16 parts of cnidium, 6-8 parts of Terminalia chebula, 13-17 parts of black sesame, 6-8 parts of black grass seed oil, 7-8 parts of sheep fat seed rice washing water fermentation liquid, 2-4 parts of catmint, and 2-4 parts of plant essential oil.

3. The anti-shedding hair growth traditional Chinese medicine composition according to claim 1, characterized in that: The plant essential oil is at least one selected from rosemary essential oil, tea tree essential oil, mint essential oil, lavender essential oil, cedar essential oil, and rose essential oil.

4. The anti-shedding hair growth traditional Chinese medicine composition according to claim 1, characterized in that: The sheep fat seed rice washing water fermentation liquid is prepared by mixing sheep fat seed rice and water, grinding and filtering, mixing the obtained filtrate with compound bacteria, and then fermenting and sterilizing, wherein the inoculation amount of the compound bacteria is 0.05-0.1% of the mass of the filtrate, and the compound bacteria contain Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis at a mass ratio of 5:1-2:0.5-1.

5. The anti-shedding hair growth promoting traditional Chinese medicine composition according to claim 4, characterized in that: The specific preparation method of the sheep fat seed rice washing water fermentation liquid is as follows: The sheep fat seed rice and water are mixed at a ratio of 1:5-10, ground and filtered to obtain a filtrate; Saccharomyces cerevisiae and Bacillus subtilis are added to the filtrate, and after fermentation at 30-37℃ for 12-24h, Lactobacillus plantarum is added, and the mixture is sealed and fermented for 12-24h, then centrifuged and sterilized at 60-70℃ for 15-30min.

6. The preparation method of the anti-shedding hair growth traditional Chinese medicine composition according to any one of claims 1-5, characterized in that: The method comprises the following steps: Cnidium, pepper and chrysanthemum leaf stem are extracted by steam to obtain cnidium oil, pepper oil and chrysanthemum leaf stem oil, respectively; Aloe is washed, dried, cut, pressed, filtered and concentrated to obtain aloe original liquid; Black sesame is crushed, and the black sesame powder is swelled in ethanol solution, then filtered and concentrated to a solid content of ≥30% to obtain black sesame extract; Terminalia chebula, u silk grass and catmint are mixed, juiced, concentrated and sterilized, then yeast is added, the pH is adjusted to 4.5-5.5, and the mixture is sealed and fermented for 1-2 months, then filtered to obtain fermentation liquid; Pepper oil, chrysanthemum stem and leaf oil and cnidium oil are mixed, and black grass seed oil and plant essential oil are added to obtain blended oil; The fermentation liquid is mixed with walnut shell vinegar, aloe original liquid and sheep fat seed rice washing water fermentation liquid to obtain blended liquid; The blended oil is added to the blended liquid, and after homogenization, black sesame extract is added, and ultrasonic dispersion is performed to obtain the anti-hair loss and hair growth traditional Chinese medicine composition.

7. The preparation method of the anti-shedding hair growth traditional Chinese medicine composition according to claim 6, characterized in that: The black sesame extract is pretreated as follows: Polylysine is added to water to prepare a polylysine solution with a concentration of 4-5wt%, and glacial acetic acid is prepared into an acetic acid solution with a concentration of 1-1.5%; The black sesame extract, polylysine solution and acetic acid solution are mixed at a mass ratio of 1:2.5-3:1.5-2, stirred uniformly, and then the pH is adjusted to 4.5-5, ultrasonic treatment is performed for 10min, and concentration is performed to a solid content of ≥30%.

8. The preparation method of the anti-shedding hair growth traditional Chinese medicine composition according to claim 6, characterized in that: The aloe juice is pretreated by a hyaluronic acid derivative, and the pretreatment method is as follows: the aloe juice is dispersed into an ethanol solution to obtain an aloe dispersion liquid, the hyaluronic acid derivative is added into the ethanol solution, the aloe dispersion liquid is added, and then stirring is performed at room temperature, and the ethanol is removed by rotary evaporation, and concentrated, and the mass ratio of the transparent derivative to the aloe juice is 5:

3.

9. The use of the anti-hair loss and hair growth traditional Chinese medicine composition according to any one of claims 1-5 or the anti-hair loss and hair growth traditional Chinese medicine composition prepared by the method of claims 6-8, characterized in that, The hair shampoo comprises the following raw materials in parts by weight: 20-40 parts of the hair growth-promoting Chinese medicine composition, 1-4 parts of poloxamer 101, 10-30 parts of a cleaning agent, 0.1-2 parts of a thickening agent, 2-5 parts of a conditioning agent, 5-10 parts of a surfactant, 0.5-1 part of a preservative, and 60-80 parts of a solvent. 10.The use of the anti-shedding hair growth traditional Chinese medicine composition according to claim 9 in the preparation of a shampoo, characterized in that: The cleaning agent is at least one selected from the group consisting of sodium fatty alcohol ether sulfate, ammonium lauryl sulfate, coconut oil diethanolamide and sodium dodecyl benzene sulfonate; The surfactant is at least one selected from the group consisting of ammonium lauryl sulfate, sodium laureth sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, cocodihydroxyethylamine and sodium laureth sulfate; The thickening agent is at least one selected from the group consisting of sodium carboxymethyl cellulose, hydroxyethyl cellulose, carbomer, gum arabic and sodium alginate; The conditioning agent is at least one selected from the group consisting of guar gum, polyquaternary ammonium salt, dimethicone, decamethylcyclopentasiloxane, guar hydroxypropyltrimonium chloride and dimethicone alcohol; The preservative is at least one selected from the group consisting of hydroxybenzoic acid ester, methylisothiazolinone, imidazolidinyl urea, phenoxyethanol and sodium benzoate; The solvent is at least one selected from the group consisting of ethanol, glycerol, propylene glycol and water.

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