Traditional Chinese medicine composition for treating atherosclerosis and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine preparation, and particularly relates to a traditional Chinese medicine composition for treating atherosclerosis and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-20 parts of radix astragali, 10-15 parts of radix salviae miltiorrhizae, 10-30 parts of fructus crataegi, 6-9 parts of radix angelicae sinensis, 6-9 parts of rhizoma chuanxiong, 10-30 parts of poria cocos, 6-9 parts of pericarpium citri reticulatae, 10-30 parts of radix puerariae, 5-10 parts of fructus lycii, 3-9 parts of radix curcumae and 3-6 parts of radix glycyrrhizae. The traditional Chinese medicine composition has the effects of resisting inflammation, resisting oxidation, regulating lipid metabolism and relieving lipid plaque.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine preparation, and particularly relates to a traditional Chinese medicine composition for treating atherosclerosis and application thereof. BACKGROUND

[0002] Atherosclerosis (AS) is a systemic disease, which is common in the elderly population. Its main feature is the accumulation of lipids and sugars in the intima of the arteries, accompanied by bleeding, thrombosis and fibrosis, which further leads to calcium deposition and thickening of the arterial wall, causing vascular stenosis. Early symptoms are often nonspecific, but patients may experience physical symptoms such as fatigue, body aches, chest tightness and shortness of breath. As the disease progresses, patients may experience angina pectoris, myocardial infarction, arrhythmia, and even sudden death; mesenteric atherosclerosis can manifest as postprandial abdominal pain, indigestion, constipation, and in severe cases, intestinal wall necrosis, hematochezia or paralytic ileus; lower extremity atherosclerosis can cause severe stenosis of the vascular lumen, resulting in intermittent claudication, foot dorsal artery pulse disappearance, and even gangrene.

[0003] Hyperlipidemia, usually refers to elevated plasma triglycerides (TG) and / or total cholesterol (TC), also includes low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). Prolonged hyperlipidemia can accelerate atherosclerosis and increase the risk of complications such as coronary heart disease and stroke, and increase the risk of cerebral infarction and cerebral hemorrhage. Hyperlipidemia can cause a series of symptoms, including chest tightness, chest pain, dizziness, claudication, and further exacerbate atherosclerosis. Studies have shown that statins can significantly reduce low-density lipoprotein cholesterol (LDL-C) levels, thereby reducing the progression of atherosclerosis and helping to reduce the risk of ASCVD. However, long-term use of statins can cause liver function abnormalities, muscle pain and other side effects, so exploring safe and effective alternative treatment options is still an important direction for current research.

[0004] Traditional Chinese medicine has unique advantages in the prevention and treatment of atherosclerosis. Studies have shown that traditional Chinese medicine can achieve significant results in the prevention and treatment of AS through the methods of promoting blood circulation and removing blood stasis, regulating qi and unblocking collaterals. Recent studies have also shown that traditional Chinese medicine compositions can exert comprehensive effects such as lipid-lowering, anti-inflammatory and antioxidant through multi-target action, and are expected to provide new solutions for the treatment of atherosclerosis.

[0005] At present, the Chinese invention patent with publication number CN115645464A discloses "a traditional Chinese medicine composition for treating atherosclerosis and fatty liver and its application", which is mainly composed of pericarpium citri reticulatae extract, cortex moutan and clove extract. However, the preparation process of each component in the prescription involves complicated pretreatment, extraction and purification and multiple process steps, which significantly increases the complexity of the production process and the difficulty of quality control, and poses a challenge to the development of standardized preparations with stable quality and high batch consistency. In addition, the Chinese invention patent with publication number CN116650606A discloses "a pharmaceutical composition for preventing and treating atherosclerosis", which is composed of rhodiola 30-35g, nitraria 10-15g, siler 10-15g, amomum xanthicoides 6-8g, apricot kernel 10-15g, platycodon 10-14g and green calyx plum 10-12g by weight. However, the prescription has a warm and pungent nature, which is easy to consume and damage yin fluid. Long-term or excessive consumption, or for patients with yin deficiency and excessive heat, can further consume yin fluid, aggravate the disease or cause new discomfort, and has strong side effects, so it cannot be taken for a long time. Therefore, the prior art needs further improvement. SUMMARY

[0006] The purpose of the present application is to provide a traditional Chinese medicine composition for treating atherosclerosis and its application.

[0007] Based on the above purpose, the present application adopts the following technical scheme: A traditional Chinese medicine composition for treating atherosclerosis, which is composed of the following raw materials by weight: 15-20 parts of radix astragali, 10-15 parts of salvia miltiorrhiza, 10-30 parts of hawthorn, 6-9 parts of angelica sinensis, 6-9 parts of ligusticum wallichii, 10-30 parts of poria cocos, 6-9 parts of pericarpium citri reticulatae, 10-30 parts of pueraria, 5-10 parts of medlar, 3-9 parts of curcuma zedoary, and 3-6 parts of licorice.

[0008] Further, it is composed of the following raw materials by weight: 15 parts of radix astragali, 10 parts of salvia miltiorrhiza, 10 parts of hawthorn, 6 parts of angelica sinensis, 6 parts of ligusticum wallichii, 10 parts of poria cocos, 6 parts of pericarpium citri reticulatae, 10 parts of pueraria, 5 parts of medlar, 3 parts of curcuma zedoary, and 3 parts of licorice.

[0009] Further, it is composed of the following raw materials by weight: 18 parts of radix astragali, 13 parts of salvia miltiorrhiza, 20 parts of hawthorn, 8 parts of angelica sinensis, 8 parts of ligusticum wallichii, 20 parts of poria cocos, 8 parts of pericarpium citri reticulatae, 20 parts of pueraria, 8 parts of medlar, 6 parts of curcuma zedoary, and 5 parts of licorice.

[0010] Further, it is composed of the following raw materials by weight: 20 parts of radix astragali, 15 parts of salvia miltiorrhiza, 30 parts of hawthorn, 9 parts of angelica sinensis, 9 parts of ligusticum wallichii, 30 parts of poria cocos, 9 parts of pericarpium citri reticulatae, 30 parts of pueraria, 10 parts of medlar, 9 parts of curcuma zedoary, and 6 parts of licorice.

[0011] An application of the above-mentioned traditional Chinese medicine composition in the treatment of atherosclerotic diseases.

[0012] Astragalus: Sweet in taste and slightly warm in nature; it enters the spleen and lung meridians. It has the effects of strengthening the spleen and replenishing the middle jiao, raising yang and lifting prolapse, benefiting wei qi and consolidating the exterior, promoting diuresis, and promoting tissue regeneration and detoxification.

[0013] Salvia miltiorrhiza: bitter in taste, slightly cold in nature; enters the heart and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain, clearing the heart and relieving irritability, cooling the blood and reducing swelling.

[0014] Hawthorn: Sour, sweet, and slightly warm; it enters the spleen, stomach, and liver meridians. It has the effects of promoting digestion and strengthening the stomach, regulating qi and dispersing blood stasis, and clearing away turbidity and lowering lipids.

[0015] Angelica sinensis: sweet and pungent in taste, warm in nature, and enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and promoting bowel movements.

[0016] Chuanxiong (Ligusticum striatum): pungent and warm in nature. It enters the liver and gallbladder meridians. It has the effects of regulating qi and relieving stagnation, dispelling wind and dampness, promoting blood circulation and relieving pain. It is mainly used to treat wind-cold headache and dizziness, hypochondriac pain and abdominal pain, cold-induced muscle spasms, amenorrhea, dystocia, postpartum blood stasis and pain, carbuncles and sores. It is also used for irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to masses, chest and hypochondriac pain, traumatic swelling and pain, headache, and rheumatic pain.

[0017] Poria cocos: Sweet and bland in taste, neutral in nature, and enters the heart, spleen, and lung meridians. It has the effects of promoting diuresis and eliminating dampness, benefiting the spleen and stomach, and calming the mind and soothing the nerves. It is mainly used to treat urinary difficulty, edema, phlegm-dampness cough, vomiting, diarrhea, seminal emission, urinary tract infections, palpitations, and forgetfulness.

[0018] Dried tangerine peel: Bitter and pungent in taste, warm in nature. It enters the spleen and lung meridians. It has the effects of regulating qi, harmonizing the middle jiao, drying dampness, and resolving phlegm. It is mainly used to treat abdominal distension, loss of appetite, vomiting, hiccups, and cough with excessive phlegm. It can also detoxify fish and crab poisoning.

[0019] Kudzu root: Sweet and pungent in taste, cool in nature. It enters the spleen and stomach meridians. It has the functions of relieving muscle tension and reducing fever, promoting body fluid production, promoting rash eruption, raising yang and stopping diarrhea. It is mainly used to treat exogenous fever with headache, stiff neck and back pain, thirst, diabetes, incomplete measles eruption, dysentery, diarrhea; and hypertension with stiff neck and back pain.

[0020] Goji berries: Sweet in taste, neutral in nature. They enter the liver and kidney meridians. They nourish the liver and kidneys, benefit essence and improve eyesight. They are used to treat weakness and deficiency of essence, lower back and knee pain, dizziness and tinnitus, internal heat and thirst, blood deficiency and chlorosis, and blurred vision.

[0021] Turmeric: pungent, bitter, and cold in nature. It enters the liver, heart, and lung meridians. It possesses the functions of promoting blood circulation and relieving pain, regulating qi and relieving depression, clearing the heart and cooling the blood, and promoting bile secretion and reducing jaundice. It is mainly used to treat stabbing pain in the chest and ribs, chest pain, amenorrhea, dysmenorrhea, breast distension and pain, delirium due to febrile diseases, epilepsy, hematemesis due to blood heat, and jaundice with dark urine.

[0022] Gancao: sweet, nature flat. Heart, lung, spleen, stomach meridian. With the effect of spleen and kidney, heat-clearing and detoxifying, expectorant and antitussive, relieving pain, regulating various drugs, for treating weak and weak, tired and tired, shortness of breath, cough and sputum, abdominal, limbs, and pain, abscess, sore.

[0023] The traditional Chinese medicine composition has the effects of anti-inflammatory, antioxidant, regulating lipid metabolism, reducing lipid plaque, and also has the effects of promoting blood circulation, regulating qi and blood, reducing lipid and reducing pressure. The traditional Chinese medicine composition of the present application has no damage to liver and kidney, good safety, and is suitable for long-term use. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is the total cholesterol (TC) level detection result graph in test example 1; Figure 2 is the triglyceride (TG) level detection result graph in test example 1; Figure 3 is the low density lipoprotein cholesterol (LDL-C) level detection result graph in test example 1; Figure 4 is the high density lipoprotein cholesterol (HDL-C) level detection result graph in test example 1; Figure 5 is the interleukin-6 (IL-6) level detection result graph in test example 1; Figure 6 is the interleukin-4 (IL-4) level detection result graph in test example 1; Figure 7 is the tumor necrosis factor-α (TNF-α) level detection result graph in test example 1. DETAILED DESCRIPTION

[0025] Example 1: A traditional Chinese medicine composition for treating atherosclerosis, which is composed of the following raw materials in parts by weight: 15g of Huangqi, 10g of Danshen, 10g of Shanzha, 6g of Danggui, 6g of Chuanxiong, 10g of Fuling, 6g of Chenpi, 10g of Gegen, 5g of Gouqi, 3g of Yujin, and 3g of Gancao.

[0026] A concentrated liquid preparation method of the traditional Chinese medicine composition, which is prepared by the following steps: (1) grinding Huangqi, Danshen, Shanzha, Danggui, Chuanxiong, Fuling, Chenpi, Gegen, Gouqi, Yujin, and Gancao into 200-300 mesh powder, then adding water and decocting at 90℃ for 1h, filtering and collecting the first filtrate; (2) adding water to the filtered residue again, decocting at 90℃ for 1h, filtering and collecting the second filtrate, and concentrating the first filtrate and the second filtrate under reduced pressure to obtain a concentrated liquid 200mL.

[0027] The concentration liquid preparation method of the traditional Chinese medicine composition, the mass-volume ratio of Astragalus, Salvia miltiorrhiza, hawthorn, angelica, Chuanxiong, Poria cocos, dried tangerine or orange peel, kudzu root, medlar, curcuma zedoary, and licorice in step (1) is 15g:10g:10g:6g:6g:10g:6g:10g:5g:3g:3g:600ml. The mass-volume ratio of the filter residue and water in step (2) is 100g:600ml.

[0028] The prepared concentration liquid is a daily dose, and 200 mL is taken per day, which is taken in 2-3 times.

[0029] The prepared concentration liquid is a daily dose, and 200 mL is taken per day, which is taken in 2-3 times.

[0030] Example 2: A traditional Chinese medicine composition for treating atherosclerosis, which is composed of the following raw materials in weight fraction: Astragalus 18g, Salvia miltiorrhiza 13g, hawthorn 20g, angelica 8g, Chuanxiong 8g, Poria cocos 20g, dried tangerine or orange peel 8g, kudzu root 20g, medlar 8g, curcuma zedoary 7g, and licorice 5g.

[0031] A concentration liquid preparation method of the traditional Chinese medicine composition, which is prepared by the following steps: (1) Astragalus, Salvia miltiorrhiza, hawthorn, angelica, Chuanxiong, Poria cocos, dried tangerine or orange peel, kudzu root, medlar, curcuma zedoary, and licorice are ground into 250-mesh powder, and then water is added to decoct at 95℃ for 1.5h, and then the first filtrate is collected after filtration; (2) the filter residue after filtration is added with water again to decoct at 95℃ for 1.5h, and then the second filtrate is collected after filtration, and the first filtrate and the second filtrate are concentrated under reduced pressure to prepare a concentration liquid of 230mL.

[0032] The concentration liquid preparation method of the traditional Chinese medicine composition, the mass-volume ratio of Astragalus, Salvia miltiorrhiza, hawthorn, angelica, Chuanxiong, Poria cocos, dried tangerine or orange peel, kudzu root, medlar, curcuma zedoary, and licorice in step (1) is 18g:13g:20g:8g:8g:20g:8g:20g:8g:7g:5g:700ml; and the mass-volume ratio of the filter residue and water in step (2) is 150g:700ml.

[0033] The prepared concentration liquid is a daily dose, and 200 mL is taken per day, which is taken in 2-3 times.

[0034] The concentrated liquid of the traditional Chinese medicine composition can be freeze-dried by a freeze dryer, then 0.2 g of the freeze-dried powder is added into 0.05 g of starch and 0.05 g of dextrin, and the mixture is uniformly mixed, and then the mixture is pressed into pills by using a pill-making machine, wherein each pill weighs 0.3 g, and the daily dosage is about 65 pills, which is orally taken in 2-3 times per day.

[0035] Example 3 A traditional Chinese medicine composition for treating atherosclerosis, which is composed of the following raw materials in parts by weight: 20 parts of Astragalus, 15 parts of Salvia miltiorrhiza, 30 parts of hawthorn, 9 parts of Angelica sinensis, 9 parts of Ligusticum chuanxiong, 30 parts of Poria cocos, 9 parts of dried tangerine or orange peel, 30 parts of Pueraria, 10 parts of Fructus lycii, 9 parts of Curcuma, and 6 parts of licorice.

[0036] A preparation method of the concentrated liquid of the traditional Chinese medicine composition, which is prepared by the following steps: (1) grinding Astragalus, Salvia miltiorrhiza, hawthorn, Angelica sinensis, Ligusticum chuanxiong, Poria cocos, dried tangerine or orange peel, Pueraria, Fructus lycii, Curcuma, and licorice into 300-mesh powder, then adding water and decocting at 100 ℃ for 2 h, filtering, and collecting the first filtrate; (2) adding the filtered residue into water again, decocting at 100 ℃ for 2 h, filtering, collecting the second filtrate, and preparing the concentrated liquid of 250 mL by concentrating the first filtrate and the second filtrate under reduced pressure.

[0037] The preparation method of the concentrated liquid of the traditional Chinese medicine composition, wherein the mass-volume ratio of Astragalus, Salvia miltiorrhiza, hawthorn, Angelica sinensis, Ligusticum chuanxiong, Poria cocos, dried tangerine or orange peel, Pueraria, Fructus lycii, Curcuma, and licorice to water in step (1) is 20 g: 15 g: 30 g: 9 g: 9 g: 30 g: 9 g: 30 g: 10 g: 9 g: 6 g: 600-800 ml; and the mass-volume ratio of the residue to water in step (2) is 200 g: 800 ml.

[0038] The prepared concentrated liquid is a daily dose, and 200 mL is taken per day, which is taken in 2-3 times per day.

[0039] The concentrated liquid of the traditional Chinese medicine composition can be freeze-dried by a freeze dryer, then 0.2 g of the freeze-dried powder is added into 0.05 g of starch and 0.05 g of dextrin, and the mixture is uniformly mixed, and then the mixture is pressed into pills by using a pill-making machine, wherein each pill weighs 0.3 g, and the daily dosage is about 65 pills, which is orally taken in 2-3 times per day.

[0040] Test Example 1 Safety of the traditional Chinese medicine composition The traditional Chinese medicine composition concentrate prepared in Example 1 was administered to mice by gavage at a crude drug equivalent of 2.5 g / kg, 5.0 g / kg, 10 g / kg, 20 g / kg, and 30 g / kg, as an experimental group, and the toxicity reaction was observed after 30 days of continuous administration. The results showed that no mice died even at a dose of 20 g / kg, and the mice with normal diet were set as a control group. After 30 days, there was no significant difference in food intake, water intake, body weight gain, blood biochemical indicators, and liver and kidney function tests between the experimental group and the control group. Since the LD 50 of the prescription is greater than 20 g / kg, which is much higher than the commonly used effective dose range, therefore, it is shown that the traditional Chinese medicine composition of the present application has no obvious toxic side effects at an effective dose, and long-term use does not cause adverse effects on the health of mice.

[0041] Test Example 2: Effectiveness of the traditional Chinese medicine composition 1. Preparation for establishment of an atherosclerosis animal model: 13 SPF male C57BL / 6J mice at 8 weeks of age, 33 ApoE - / - mice with a body weight of (22±2) g were purchased from Jiangsu Jizhuangkang Biotechnology Co., Ltd. (Animal Production License No. SCXK2023-0009). They were raised in the Animal Experiment Center of Henan University of Chinese Medicine (License No. SYXK(YU)2021-0015) at a temperature of (22±2) °C, a humidity of (50±10) %, and a 12h light / dark cycle. The animals were allowed to freely eat and drink. The animal ethics review approval number was No. DWLLGZR202303167. Western diet feed and ordinary feed were provided by Beijing Huafukang Biotechnology Co., Ltd. (License No. SCXK(Jing)2019-0008). The animals were modeled, grouped, and administered: After 1 week of adaptive feeding, 13 C57BL / 6J mice were used as a control group and were fed with ordinary maintenance feed throughout the experiment. The model group consisted of 33 ApoE - / - mice, which were fed with western diet feed. After 8 weeks of modeling, 3 mice from each of the control and model groups were randomly selected for observation: The aortic gross oil red O staining showed that the aortic plaque of the model mice was obviously formed, which was significantly different from that of the control group, indicating that the AS in vivo model was successfully established.

[0042] The 30 ApoE - / -The mice were randomly divided into 3 groups: a model group, an experimental group (Example 1 group), and a positive drug group, with 10 mice in each group, and the mice were continuously fed with western diet feed. Except for the model group, all the medicated groups of mice were fed with western diet feed, and at the same time, the corresponding drugs were administered by gavage (0.2 ml per mouse per day); the control group and the model group were administered with the same volume of normal saline by gavage to ensure consistency between groups. Gavage was performed once a day for 8 consecutive weeks. The specific intervention measures are shown in Table 1 below: Table 1: Drug administration scheme used in this experiment Grouping Feeding conditions Mice Intervention Gavage amount Control group Common feed feeding Male C57BL / 6J Gavage administration of equal volume of normal saline 0.2 ml / each / day Model group Western diet feed feeding Male isogenic ApoE - / - ]] Gavage administration of equal volume of normal saline 0.2 ml / each / day Positive drug group Western diet feed feeding Male isogenic ApoE - / - ]]> Gavage administration of atorvastatin aqueous solution 10 mg / kg / d 0.2 ml / each / day Example 1 group Western diet feed feeding Male isogenic ApoE - / - ]]> Gavage administration of example 1 concentrate 0.2 ml / each / day

[0043] Note: The drug administration dose is converted into the gavage administration dose for mice according to the clinical recommended dose of oral administration for adults of 70 kg per day and the body surface area conversion factor between adults and mice.

[0044] 2. Collection of serum and tissue specimens After 8 weeks of administration, after the last administration, the mice were fasted for 12 hours without water. Anesthesia was performed by inhaling 4% isoflurane, and 150 μL of blood was taken from the retrobulbar vein (orbital venous plexus). The blood was allowed to stand at room temperature for 2 hours, then centrifuged at 4000 r / min at 4°C for 10 minutes. The upper serum was separated and stored at -80°C. After taking the blood, the mouse chest and abdominal cavity were opened, and the aorta was isolated from the aortic root to the common iliac artery bifurcation, and the surrounding connective tissue and adipose tissue were stripped. The aorta was then immersed in liquid nitrogen or tissue fixative for preservation for subsequent experiments.

[0045] 3. Enzyme-labeled method for detecting serum lipid levels in mice The levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in the serum of mice were detected using a fully automatic biochemical analyzer. The results are shown below Figure 1 As shown in Figures 1-4 Example 1 of the present application can increase the level of HDL-C, reduce the levels of TC, TG, and LDL-C in mice, and is significantly superior to the positive drug group in reducing LDL-C, thereby treating atherosclerosis.

[0046] 4. ELISA method for detecting serum inflammatory factor levels The levels of IL-6, IL-4, and tumor necrosis factor-α in the serum of mice in the control group, the model group, the positive drug group, and Example 1 group were detected using the ELISA kit instructions. The results are shown below Figures 5-7 As shown inExample 1 can increase the level of anti-inflammatory factors and reduce the level of pro-inflammatory factors, and Example 1 is superior to the positive drug group in increasing the level of anti-inflammatory factors.

[0047] 5. Gross oil red O staining to determine the whole aortic plaque area The longitudinal section of mouse aorta was fixed under optical microscope, washed with water, stained with oil red O, and then the blood vessel was laid on a black rubber plate, quickly photographed, and analyzed by ImageJ software to evaluate the severity of aortic plaque by aortic relative plaque area. Statistical analysis and statistical mapping were performed using GraphPad Prism, and the experimental results were expressed as mean ± standard deviation (± SD). The mean values between groups were compared by One-Way ANOVA analysis, and the difference was considered statistically significant when the significance probability P was less than 0.05.

[0048] The analysis results are as follows: The relative plaque area (%) = intimal plaque area of aorta / total vessel area × 100%. The results are shown in Table 2 as follows: Table 2: Relative plaque area (%) of mouse aorta by gross oil red O staining ± s, n = 4.

[0049] Grouping Plaque area Control group 4.36±0.58 Model group 15.63 ± 1.34 *△ ]] Positive drug group 13.48 ± 0.83 * ]] Example 1 group 5.17 ± 0.75 △ ]]

[0050] Among them, represent the mean, s represents the standard deviation; n represents the sample number; P represents the significance level of the test result. In Table 2, P<0.05 compared with the control group; P<0.05 compared with the model group. As can be seen from the above, the aortic total plaque area of Example 1 group can be reduced, which is better than the comparative example group and the positive drug group. Therefore, the present application has obvious effect on treating atherosclerosis.​

Claims

1. A traditional Chinese medicine composition for treating atherosclerosis, characterized in that, It is composed of the following ingredients in parts by weight: Astragalus membranaceus 15-20 parts, Salvia miltiorrhiza 10-15 parts, Crataegus pinnatifida 10-30 parts, Angelica sinensis 6-9 parts, Ligusticum chuanxiong 6-9 parts, Poria cocos 10-30 parts, Citrus reticulata 6-9 parts, Pueraria lobata 10-30 parts, Lycium barbarum 5-10 parts, Curcuma longa 3-9 parts, Glycyrrhiza uralensis 3-6 parts.

2. The traditional Chinese medicine composition for treating atherosclerosis according to claim 1, characterized in that, It is composed of the following ingredients in parts by weight: Astragalus membranaceus 15 parts, Salvia miltiorrhiza 10 parts, Crataegus pinnatifida 10 parts, Angelica sinensis 6 parts, Ligusticum chuanxiong 6 parts, Poria cocos 10 parts, Citrus reticulata 6 parts, Pueraria lobata 10 parts, Lycium barbarum 5 parts, Curcuma longa 3 parts, Glycyrrhiza uralensis 3 parts.

3. The traditional Chinese medicine composition for treating atherosclerosis according to claim 1, characterized in that, It is composed of the following ingredients in parts by weight: Astragalus membranaceus 18 parts, Salvia miltiorrhiza 13 parts, Crataegus pinnatifida 20 parts, Angelica sinensis 8 parts, Ligusticum chuanxiong 8 parts, Poria cocos 20 parts, Citrus reticulata 8 parts, Pueraria lobata 20 parts, Lycium barbarum 8 parts, Curcuma longa 6 parts, Glycyrrhiza uralensis 5 parts.

4. The traditional Chinese medicine composition for treating atherosclerosis according to claim 1, characterized in that, It is composed of the following ingredients in parts by weight: Astragalus membranaceus 20 parts, Salvia miltiorrhiza 15 parts, Crataegus pinnatifida 30 parts, Angelica sinensis 9 parts, Ligusticum chuanxiong 9 parts, Poria cocos 30 parts, Citrus reticulata 9 parts, Pueraria lobata 30 parts, Lycium barbarum 10 parts, Curcuma longa 9 parts, Glycyrrhiza uralensis 6 parts.

5. A method for preparing a concentrated solution of the traditional Chinese medicine composition according to claim 1, characterized in that, It is prepared by the following steps: (1) Astragalus membranaceus, Salvia miltiorrhiza, hawthorn, Angelica sinensis, Ligusticum chuanxiong, Poria cocos, Citrus reticulata peel, Pueraria lobata, Lycium barbarum, Curcuma longa and Glycyrrhiza uralensis are pulverized into powder of 200-300 mesh, then water is added and decocted at 90-100℃ for 1-2 hours, then filtered and the first filtrate is collected; (2) The filtered residue is added to water again and decocted at 90-100℃ for 1-2 hours, then filtered and the second filtrate is collected. The first filtrate and the second filtrate are concentrated under reduced pressure to obtain a concentrated solution of 200-250 mL.

6. The method for preparing a concentrated solution of the traditional Chinese medicine composition according to claim 1, characterized in that, In step (1), the mass-to-volume ratio of Astragalus membranaceus, Salvia miltiorrhiza, Crataegus pinnatifida, Angelica sinensis, Ligusticum chuanxiong, Poria cocos, Citrus reticulata peel, Pueraria lobata, Lycium barbarum, Curcuma longa, Glycyrrhiza uralensis and water is 15-20g: 10-15g: 10-30g: 6-9g: 6-9g: 10-30g: 6-9g: 10-30g: 5-10g: 3-9g: 3-6g: 600-800ml; in step (2), the mass-to-volume ratio of filter residue and water is 100-200g: 600-800ml.

7. An application of the traditional Chinese medicine composition according to any one of claims 1-4 in the treatment of atherosclerosis.

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