Avocado fermented composition, method for preparing the same, and use thereof

Abstract

The application belongs to the technical field of cosmetics, and discloses an avocado fermentation composition and a preparation method and application thereof. The composition comprises 1%-10% of avocado fermentation filtrate, 0.1%-2% of alpha-glucan oligosaccharide, 0.1%-2% of wild chrysanthemum extract, 3%-8% of a humectant, 0.01%-0.1% of a chelating agent, and the rest of deionized water. The avocado fermentation filtrate is obtained by complex fermentation of aphid pseudomycetes and candida fungi, and is rich in oligopeptides, free amino acids, lactic acid and polyphenols. The application scientifically compounds the filtrate obtained by fermenting avocados with specific complex strains and prebiotics with clear bacterial flora selectivity, and constructs a multifunctional composition with clear structure, synergistic function and high stability. The composition not only provides direct barrier repair and moisturizing effect, but also actively guides the skin micro-ecosystem to healthy succession through nutritional intervention, thereby synchronously improving the skin health level in two dimensions of molecules and ecology.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and relates to a fermented avocado composition and a preparation method and application thereof. BACKGROUND

[0002] With the deepening of consumers' understanding of skin health, cosmetic research and development has gradually evolved from traditional surface physicochemical intervention to deeper biological mechanism exploration. As the largest organ of the human body, the skin's homeostasis depends not only on the physical barrier function of the stratum corneum, but also on the complex microbial community inhabiting it, i.e., the skin microecology.

[0003] In recent years, the balance of skin microecology has been confirmed to play a key role in maintaining barrier integrity, regulating immune response and resisting external stimuli, which has given rise to the rise of the concept of microecologically friendly skin care. Under this background, how to effectively regulate skin flora structure, promote the proliferation of beneficial bacteria and inhibit potential pathogenic bacteria through cosmetic ingredients has become an important direction for the development of functional skin care products.

[0004] Traditional cosmetics focus on chemically caring for the skin through small molecule moisturizing agents (such as glycerol, hyaluronic acid), lipid mimics or antioxidant ingredients, and their mechanisms of action are mainly limited to surface repair pathways such as replenishing water, occlusive moisturizing or scavenging free radicals. Although such technologies can improve skin dryness, roughness and other appearance problems in the short term, they do not touch the root of skin health - the dynamic balance of the microecosystem. Moreover, a single active ingredient often cannot meet multiple functional dimensions: for example, some strong moisturizers may change the skin surface pH or osmotic pressure, indirectly interfering with microbial colonization; while some plant extracts with antibacterial activity can inhibit harmful bacteria, but may indiscriminately inhibit the symbiotic flora, thereby disrupting the microecological homeostasis. SUMMARY

[0005] To achieve the above-mentioned purposes, the present application provides a fermented avocado composition and a preparation method and application thereof. The composition is prepared by scientifically compounding the fermented avocado product obtained by fermenting specific strains with prebiotics with clear microecological regulation function, thereby constructing a synergistic system with dual functions of postbiotics and prebiotics, so as to realize skin barrier repair and moisturizing at the molecular level and active regulation of skin microecological homeostasis at the ecological level.

[0006] The avocado fermentation composition comprises the following components: avocado fermentation filtrate, alpha-glucan oligosaccharide, wild chrysanthemum extract, humectant, chelating agent and deionized water; wherein the mass fraction of the avocado fermentation filtrate is 1% to 10%, the mass fraction of the alpha-glucan oligosaccharide is 0.1% to 2%, the mass fraction of the wild chrysanthemum extract is 0.1% to 2%, the mass fraction of the humectant is 3% to 8%, the mass fraction of the chelating agent is 0.01% to 0.1%, and the balance is deionized water.

[0007] The avocado fermentation filtrate is prepared by the following steps: first, select the Hass variety avocado with maturity of 7 to 8 levels, fruit flesh complete without browning, after peeling and coring, the fruit flesh is chopped and added with 3 times the mass of deionized water, and homogenized at 4℃ to obtain an avocado homogenate; then, the avocado homogenate is filtered through a 100 mesh screen to remove coarse fiber residues to obtain a clear avocado substrate liquid; then, the avocado substrate liquid is inoculated with a complex fermentation strain, the complex fermentation strain is mixed by aphid-like yeast and Candida fungi at an active bacteria ratio of 1:1, and the inoculation amount is 2% of the total volume of the avocado substrate liquid; the fermentation process is carried out at 30℃ under anaerobic conditions for 72 hours, and the pH value is monitored every 24 hours during the period, and the fermentation is terminated when the pH value is reduced to below 4.2; after the fermentation is completed, the fermentation liquid is centrifuged at 4℃ and 8000 rpm for 15 minutes, the supernatant is taken, and then filtered through a 0.22 micron microporous filter to remove bacteria to obtain the avocado fermentation filtrate.

[0008] The aphid-like yeast is derived from China General Microbiological Culture Collection Center, numbered CGMCC No. 31644.

[0009] The Candida fungi is derived from China Industrial Microbial Culture Collection Center, numbered CICC 32834.

[0010] The avocado fermentation filtrate contains oligopeptides, free amino acids, organic acids and polyphenols with a molecular weight less than 1000 daltons; wherein the total free amino acid content is not less than 800 mg / L, mainly containing serine, proline, glycine and glutamic acid, and the molar ratio is 25%, 20%, 18% and 15% respectively; the total organic acid content is not less than 1.5 g / L, mainly lactic acid, accounting for not less than 70%; the total polyphenol content is not less than 300 mg / L, mainly catechin, epicatechin and procyanidin B2; the average molecular weight of the oligopeptide is 650 daltons, and it has the biological activity of promoting keratinocyte migration and tight junction protein expression.

[0011] The alpha-glucan oligosaccharide is a glucose polymer connected by alpha-1, 6-glycosidic bond, the polymerization degree is 2-10, the average molecular weight is 1200 Dalton, the proliferation promotion rate of Staphylococcus epidermidis is not less than 40% at a concentration of 0.5%, and the inhibition rate of Staphylococcus aureus is not less than 30%; the wild chrysanthemum extract is a linear fructan composed of beta-2, 1-fructose units, the polymerization degree is 10-30, the average molecular weight is 5000 Dalton, and the relative abundance of Bifidobacterium on the skin surface can be significantly improved by 25% or more at a concentration of 1%.

[0012] The humectant is a mixture of 1, 3-propanediol and panthenol, and the mass ratio of the two is 2:1; the purity of the 1, 3-propanediol is not less than 99.5%, and the 1, 3-propanediol has the effects of inhibiting the release of histamine and reducing the rate of transdermal water loss; the panthenol can be converted into pantothenic acid on the skin surface, participate in the synthesis of coenzyme A, promote lipid metabolism and barrier repair.

[0013] As another preferred embodiment of the present application, the humectant is avocado fruit extract, which is a natural raw material and rich in various bioactive ingredients, and has the effect of moisturizing and nourishing the skin.

[0014] The chelating agent is disodium ethylenediaminetetraacetate, which can effectively complex metal ions, prevent the oxidation and discoloration of polyphenols, and maintain the long-term stability of the composition within the pH range of 4.5-5.5 when the concentration is controlled at 0.05%.

[0015] As another preferred embodiment of the present application, the avocado fermentation composition further comprises 0.5% of ceramide NP and 0.3% of cholesterol, which together with the free fatty acids in the avocado fermentation filtrate form a biomimetic lipid system that can be embedded in the lipid bilayer of the stratum corneum, repairing the barrier structure defects caused by microecological imbalance.

[0016] As another preferred embodiment of the present application, the avocado fermentation composition further comprises tetrahydro methyl pyrimidine carboxylic acid, which has the effects of moisturizing, repairing skin barrier and enhancing skin tolerance.

[0017] The preparation method of the avocado fermentation composition of the present application comprises the following steps:

[0018] First, the deionized water is heated to 75℃, and then 1, 3-propanediol, panthenol and disodium ethylenediaminetetraacetate are added in sequence, and stirred until completely dissolved to form an aqueous phase;

[0019] Subsequently, the aqueous phase is cooled to below 40℃, and then the avocado fermentation filtrate, alpha-glucan oligosaccharide and wild chrysanthemum extract are added in sequence, and dissolved for 30 minutes under the condition of stirring at 300 rpm to ensure that the prebiotics are completely dispersed;

[0020] If the formula contains ceramide NP and cholesterol, they need to be dissolved in a small amount of 1,3-propanediol in advance to form a lipid premix, then slowly added to the above-mentioned aqueous phase at 40℃, homogenized (8000 rpm for 5 minutes), and then cooled to 25℃ to obtain the finished product.

[0021] The application of the avocado fermented composition of the present application is specifically adding it as an active ingredient in skin care lotion, serum, cream or mask, and the addition amount is 5% to 20% of the total mass of the composition; in the application, the composition plays a role through the following mechanisms:

[0022] Firstly, the oligopeptides and free amino acids in the avocado fermented filtrate can be directly absorbed by keratinocytes, up-regulating the mRNA expression levels of filaggrin and loricrin, and enhancing the structural integrity of the stratum corneum;

[0023] Secondly, the lactic acid produced by fermentation can slightly reduce the pH value of the skin surface to about 5.0, creating a slightly acidic environment that is not conducive to the colonization of pathogenic bacteria;

[0024] Thirdly, alpha-glucan oligosaccharides and wild chrysanthemum extract act as specific prebiotics, selectively promoting the proliferation of Staphylococcus epidermidis and Bifidobacterium, and inhibiting the excessive growth of Staphylococcus aureus and Propionibacterium acnes;

[0025] Fourthly, polyphenols play an anti-inflammatory role by inhibiting the NF-κB signaling pathway and reducing the secretion levels of IL-6 and TNF-α;

[0026] Fifthly, 1,3-propanediol and panthenol synergistically reduce the rate of transepidermal water loss and increase the water content of the stratum corneum.

[0027] Further, the composition of the present application controls the fermentation end point pH value and centrifugation parameters during preparation to ensure that the fermentation product does not contain live bacteria and macromolecular protein impurities, avoiding triggering skin immune response; at the same time, by limiting the polymerization degree and molecular weight range of prebiotics, it ensures that they have sufficient residence time on the skin surface to play a nutritional role, rather than being rapidly washed out or metabolized.

[0028] Compared with the prior art, the present application has the following beneficial effects:

[0029] The present application constructs a multifunctional composition with clear structure, synergistic function and high stability by scientifically compounding the filtrate obtained by fermenting avocado with specific composite strains with clear bacterial population selectivity and prebiotics with clear bacterial population selectivity; the composition not only provides direct barrier repair and moisturizing effects, but also actively guides the succession of skin microecology to a healthy steady state through nutritional intervention, thereby simultaneously improving the skin health level in two dimensions of molecules and ecology, effectively solving the technical problem of fragmented functions of moisturizing, repair and microecological regulation in the prior art. DETAILED DESCRIPTION

[0030] The application relates to an avocado fermentation composition and a preparation method and application thereof, which comprises the following steps: fermenting specific complex strains to obtain an avocado fermentation filtrate, and scientifically compounding the avocado fermentation filtrate with prebiotics with definite micro-ecological regulation functions to construct a synergistic system with double functions of postbiotics and prebiotics, so that skin barrier repair and deep moisturizing are realized at a molecular level, and active regulation of skin micro-ecological homeostasis is realized at an ecological level. The composition not only provides direct biological active substances to enhance the structural integrity of the stratum corneum, but also guides the succession of skin flora in a healthy direction through specific nutritional intervention, effectively solving the technical problem of functional fragmentation among moisturizing, repair and micro-ecological regulation of existing cosmetics.

[0031] The technical scheme of the application will be described in detail below in combination with specific examples and comparative examples, so that those skilled in the art can fully understand and realize the application.

[0032] Example 1: components and mass fractions: avocado fermentation filtrate 5%, alpha-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 86.95%; preparation process: according to the method, deionized water is heated to 75 DEG C, 1,3-propanediol, panthenol and disodium ethylenediaminetetraacetate are added and stirred and dissolved, and then cooled to below 40 DEG C, and then the avocado fermentation filtrate, alpha-glucan oligosaccharide and wild chrysanthemum extract are added, and stirred at 300 r / min for 30 min, and then obtained.

[0033] Example 2: components and mass fractions: avocado fermentation filtrate 10%, alpha-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 81.95%; preparation process: same as example 1.

[0034] Example 3: components and mass fractions: avocado fermentation filtrate 1%, alpha-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 90.95%; preparation process: same as example 1.

[0035] Example 4: components and mass fractions: avocado fermentation filtrate 5%, alpha-glucan oligosaccharide 2%, wild chrysanthemum extract 0.1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 86.85%; preparation process: same as example 1.

[0036] Example 5: Components and mass fraction: avocado fermentation filtrate 5%, a-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, ceramide NP 0.5%, cholesterol 0.3%, deionized water 86.15%; preparation process: ceramide NP and cholesterol are dissolved in part of 1,3-propanediol to form a lipid premix, and the remaining steps are the same as example 1. After adding the water phase at 40°C, homogenize at 8000 rpm for 5 minutes, and cool to 25°C.

[0037] Example 6: Components and mass fraction: avocado fermentation filtrate 5%, a-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.01%, deionized water 86.99%; preparation process: same as example 1.

[0038] Example 7: Components and mass fraction: avocado fermentation filtrate 5%, a-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.1%, deionized water 86.9%; preparation process: same as example 1.

[0039] Example 8: Components and mass fraction: avocado fermentation filtrate 5%, a-glucan oligosaccharide 0.1%, wild chrysanthemum extract 2%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 85.85%; preparation process: same as example 1.

[0040] Example 9: The difference between example 9 and example 1 is that example 9 uses an equal amount of avocado fruit extract to replace the 1,3-propanediol and panthenol mixture, and the others are the same.

[0041] Example 10: The difference between example 10 and example 1 is that 5% of tetrahydro methyl pyrimidine carboxylic acid is added in example 10, and the deionized water content is 81.95%, and the others are the same.

[0042] Comparative example 1: Components and mass fraction: avocado fermentation filtrate 0%, a-glucan oligosaccharide 1%, wild chrysanthemum extract 1%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 91.95%; preparation process: same as example 1.

[0043] Comparative example 2: Components and mass fraction: avocado fermentation filtrate 5%, a-glucan oligosaccharide 0%, wild chrysanthemum extract 0%, 1,3-propanediol 4%, panthenol 2%, disodium ethylenediaminetetraacetate 0.05%, deionized water 88.95%; preparation process: same as example 1.

[0044] Test method:

[0045] 1. Accelerated stability test:

[0046] Each composition was placed in a constant temperature environment of 45°C for 3 months to observe whether precipitation or discoloration occurred. Microbial contamination was detected using microbial culture method. The initial and 3-month pH values were measured using a pH meter, and the pH fluctuation value was calculated.

[0047] 2. Reconstructed human epidermis model test:

[0048] Each composition was applied to the reconstructed human epidermis model at a concentration of 10% and cultured for 48 hours. The expression of filaggrin was detected using immunoblotting, and the fold increase relative to the blank group was calculated. The transdermal water loss rate was measured using a transdermal water loss meter, and the reduction rate relative to the blank group was calculated.

[0049] 3. In vitro microecological co-culture test:

[0050] Each composition was added to a mixed bacterial solution containing Staphylococcus epidermidis and Staphylococcus aureus (initial bacterial amount ratio 1:1) at a concentration of 5% and cultured for 24 hours. The number of colonies of the two bacteria was counted using plate counting method, and the proportion of Staphylococcus epidermidis and Staphylococcus aureus was calculated.

[0051] 4. Human patch test:

[0052] Thirty healthy subjects were selected, and the inside of the forearm was applied with serum containing 10% of each composition once a day for 28 consecutive days. The skin moisture content before and after use was measured using a skin moisture tester, and the increase rate was calculated. The transdermal water loss rate before and after use was measured using a transdermal water loss meter, and the reduction rate was calculated. The skin surface flora was detected using 16S rRNA sequencing method, and the relative abundance increase value of Staphylococcus epidermidis and the relative abundance decrease value of Staphylococcus aureus were calculated. Skin reactions were recorded to determine irritation.

[0053] The stability, filaggrin fold increase, in vitro transdermal water loss rate reduction, Staphylococcus epidermidis proportion, and Staphylococcus aureus proportion of Comparative Examples 1-8 and Example 1-2 were tested, as shown in Table 1.

[0054] The human skin moisture content increase rate, human transdermal water loss rate reduction, Staphylococcus epidermidis abundance increase value, Staphylococcus aureus abundance decrease value, and irritation of Comparative Examples 1-8 and Example 1-2 were tested, as shown in Table 2.

[0055] Table 1

[0056]

[0057] Table 2

[0058]

[0059] The stability of Examples 1, 2, 3, 4, 5, 8, 9, and 10 is good, without precipitation, discoloration, microbial contamination, and small pH fluctuation; Example 6 has a pH fluctuation of 0.3 and a slight discoloration due to the insufficient complexation of metal ions caused by the ethylenediaminetetraacetic acid disodium content of only 0.01%; and Example 7 has a slight skin irritation in some subjects, although the stability meets the standard, because the ethylenediaminetetraacetic acid disodium content is 0.1%. Therefore, when the mass fraction of ethylenediaminetetraacetic acid disodium is 0.05%, the stability and skin safety of the composition achieve the best balance.

[0060] All examples containing avocado fermentation filtrate have significantly better silk fibroin expression fold and transdermal water loss rate reduction than Comparative Example 1 without fermentation filtrate. Example 2 has the highest content of avocado fermentation filtrate (10%), the silk fibroin is increased to 2.8 times, and the in vitro transdermal water loss rate is reduced by 38%, which is the best barrier repair effect in all examples; Example 5 has a bionic lipid system constructed by adding ceramide NP and cholesterol, and the in vitro transdermal water loss rate is reduced by 40% and the human transdermal water loss rate is reduced by 32%, which are better than other examples without this component, further proving that bionic lipids can strengthen the barrier repair effect; Comparative Example 1 has only a 1.0-fold increase in silk fibroin and a less than 15% reduction in transdermal water loss rate due to the lack of low peptides and free amino acids in avocado fermentation filtrate, indicating that avocado fermentation filtrate is the core component for achieving barrier repair and long-term moisturizing.

[0061] Examples containing α-glucan oligosaccharides and wild chrysanthemum extract have much better Staphylococcus epidermidis proportion, abundance increase value, and Staphylococcus aureus proportion, abundance decrease value than Comparative Example 2 without prebiotics. Example 4 has a 2% content of α-glucan oligosaccharides, the Staphylococcus epidermidis proportion is increased to 85%, and the abundance is increased by 34%; Example 8 has a 2% content of wild chrysanthemum extract, the Staphylococcus epidermidis abundance is increased by 35%, which reflects the positive effect of prebiotic content on microecological regulation; Comparative Example 2 has a Staphylococcus epidermidis proportion of only 55% and an abundance increase of only 5% due to the lack of prebiotics, which cannot effectively inhibit harmful bacteria, proving that α-glucan oligosaccharides and wild chrysanthemum extract are the key components for achieving microecological homeostasis regulation.

[0062] Embodiments 1, 2, 4, 5, 8, 9, and 10 have excellent barrier repair, moisturizing, and microecological regulation functions, while Comparative Example 1 can only slightly regulate microecology, and lacks barrier and moisturizing functions; Comparative Example 2 can only repair the barrier, and the microecological regulation function is ineffective, and both have the problem of fragmentation of moisturizing-repair-microecological regulation functions. The present application solves the functional fragmentation problem of the prior art by synergistically compounding avocado fermentation filtrate (providing postbiotics) and alpha-glucan oligosaccharide, wild chrysanthemum extract (providing prebiotics), which simultaneously acts at the molecular and ecological levels, effectively solving the functional fragmentation problem of the prior art, and the indicators of embodiment 1 are balanced and excellent, and it is the preferred formula with the best comprehensive performance.

[0063] The above is only a preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A fermented avocado composition, characterized in that, The composition comprises the following components by mass fraction: avocado ferment filtrate 1%-10%, alpha-glucan oligosaccharide 0.1%-2%, wild chrysanthemum extract 0.1%-2%, humectant 3%-8%, ethylenediaminetetraacetic acid disodium salt 0.05%, ceramide NP 0.5%, cholesterol 0.3%, and the balance deionized water; The avocado ferment filtrate is obtained by homogenizing avocado pulp, inoculating a complex fermentation strain composed of aphid pseudomycetes and Candida fungi at a viable bacterial number ratio of 1:1, and fermenting under anaerobic conditions at 30°C until the pH value is less than 4.2, followed by centrifugation and 0.22 micron membrane filtration; The aphid pseudomycetes is derived from the China General Microbiological Culture Collection Center, with the number CGMCC No. 31644; The Candida fungi is derived from the China Industrial Microbial Culture Collection Center, with the number CICC 32834; The alpha-glucan oligosaccharide is an alpha-1,6-glycosidic bond connected glucose polymer, and the wild chrysanthemum extract is a beta-2,1-fructose linear polymer; The humectant is a mixture of 1,3-propanediol and panthenol at a mass ratio of 2:

1.

2. The avocado fermented composition according to claim 1, characterized in that, The free amino acids in the avocado ferment filtrate include serine, proline, glycine, and glutamic acid, with molar ratios of 25%, 20%, 18%, and 15%, respectively.

3. A process for the preparation of a fermented avocado composition according to any one of claims 1 to 2, characterized in that, The method comprises the following steps: Heat the deionized water to 75°C, add 1,3-propanediol, panthenol, and ethylenediaminetetraacetic acid disodium salt, and stir to dissolve to form an aqueous phase; After cooling to below 40°C, sequentially add the avocado ferment filtrate, alpha-glucan oligosaccharide, and wild chrysanthemum extract, and stir at 300 rpm for 30 minutes; First, dissolve ceramide NP and cholesterol in part of the 1,3-propanediol to form a lipid premix, slowly add the aqueous phase at 40°C, and then homogenize at 8000 rpm for 5 minutes before cooling to 25°C.

4. Use of the avocado fermented composition according to any one of claims 1 to 3 in a skin care product, characterized in that, The composition is added as an active ingredient at a mass fraction of 5% to 20% in skin care emulsion, serum, cream, or mask, for repairing skin barrier, moisturizing, and regulating skin microecological homeostasis.

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