A high-activity rhodiola extract freeze-dried powder, a preparation method and application thereof

Abstract

The application provides a high-activity rhodiola extract freeze-dried powder and a preparation method and application thereof.The high-activity rhodiola extract freeze-dried powder is composed of the following components in parts by weight: rhodiola extract: 10-20 parts; mannose: 78-89 parts; xanthan gum: 1-2 parts; and other pharmaceutically acceptable adjuvants: 0-10 parts. The rhodiola extract, mannose and xanthan gum are compounded in the application, wherein the xanthan gum and the mannose are not only simple supporting agents and protective agents, but also have a synergistic effect with the rhodiola extract, and the activity is improved by 1+1+1>3. Experimental data prove that the anti-inflammatory effect of the high-activity rhodiola extract freeze-dried powder is significantly improved, and the analgesic activity is well maintained.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical technology, specifically to a highly active Rhodiola rosea extract freeze-dried powder, its preparation method, and its application. Background Technology

[0002] Rhodiola rosea is a traditional and precious Chinese medicinal herb. Its main active ingredients include rhodioloside, tyrosol, polysaccharides, and flavonoids. It has excellent antioxidant, anti-fatigue, anti-hypoxia, immune-enhancing, and cardiovascular-protective effects and is widely used in pharmaceuticals, functional foods, and cosmetics. However, Rhodiola rosea products on the market (such as ordinary extracts and powders made from simple physical mixtures) have the following technical defects: (1) Unstable active ingredients: Rhodioloside and other glycosides are sensitive to light, heat, and humidity and are easily degraded during storage, resulting in a decrease in product activity; (2) Low bioavailability: Conventional preparations may have poor solubility and permeability, affecting in vivo absorption and failing to fully exert their pharmacological effects; (3) Single or limited efficacy: It relies solely on the activity of Rhodiola rosea itself, and the upper limit of efficacy is limited by the quality of the extract itself. There are no reports of significantly improving its activity through formulation technology. Although freeze-drying technology can better preserve heat-sensitive components, the freeze-drying process alone cannot solve the problems of bioavailability and synergistic effects of active ingredients. Therefore, developing a novel formulation that can significantly enhance the activity, stability, and absorption efficiency of Rhodiola rosea is of significant market value and technical necessity. Summary of the Invention

[0003] Technical Problem to be Solved: To address the aforementioned technical problems, the objective of this invention is to provide a highly active Rhodiola rosea extract freeze-dried powder, its preparation method, and its applications. This invention utilizes a ternary composite of Rhodiola rosea extract, mannose, and xanthan gum. Xanthan gum and mannose are not merely simple support and protectants; they exhibit a synergistic effect with Rhodiola rosea extract, achieving an activity enhancement effect greater than the sum of its parts (1+1+1>3). Experimental data demonstrate that the highly active Rhodiola rosea extract freeze-dried powder of this invention significantly enhances anti-inflammatory activity while maintaining its analgesic activity well.

[0004] Technical solution: A highly active Rhodiola rosea extract freeze-dried powder, composed of the following components, in parts by weight: Rhodiola rosea extract: 10-20 parts; Mannose: 78-89 parts; Xanthan gum: 1-2 parts; Other pharmaceutically acceptable excipients: 0-10 parts.

[0005] Furthermore, the aforementioned highly active Rhodiola rosea extract freeze-dried powder is composed of the following ingredients, in parts by weight: Rhodiola rosea extract: 20 parts; Mannose: 78 parts; Xanthan gum: 2 parts.

[0006] Furthermore, the extraction method of the Rhodiola rosea extract is as follows: (1) Take dried Rhodiola rosea, grind it to 200 mesh to obtain Rhodiola rosea fine powder; (2) Soak Rhodiola rosea powder in 70% ethanol at a mass-volume ratio of 1g:10mL overnight; (3) Stir at 60℃ and 150-200rpm for 8 hours, then filter to obtain the extract; (4) Concentrate under pressure at a vacuum of 0.06-0.08 MPa and a temperature of 50-60℃ until the solid content is ≥50%, and then dry to obtain the product.

[0007] Furthermore, the other pharmaceutically acceptable excipients include vitamin C and maltodextrin.

[0008] The preparation method of the above-mentioned highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix part of the mannose and xanthan gum, add an appropriate amount of water, stir until semi-dissolved, homogenize at high speed for the first time, add water to 5 times the total volume, add the remaining mannose while stirring, stir until dissolved, slowly add Rhodiola rosea extract, stir evenly, homogenize at high speed for the second time to obtain the mixture. S2. Freeze-drying: Pre-freeze the mixture at -40℃ or below for 4-6 hours, then freeze-dry until the moisture content is ≤5% to obtain freeze-dried blocks; S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0009] Furthermore, the first high-speed homogenization condition in S1 is 10,000 rpm for 10-12 min; the second high-speed homogenization condition is 10,000 rpm for 10-15 min.

[0010] Furthermore, the stirring speed in S1 is 500-600 rpm.

[0011] Furthermore, the freeze-drying conditions in S2 are as follows: under a vacuum degree of less than 20 Pa, the first stage of drying is carried out at -20℃ to 0℃ for 10-20 hours, followed by the second stage of desorption drying at 20-30℃ for 4-8 hours.

[0012] The application of the above-mentioned highly active Rhodiola rosea extract freeze-dried powder or the highly active Rhodiola rosea extract freeze-dried powder prepared by the above-mentioned method in the preparation of antioxidant, anti-inflammatory, analgesic or immune-enhancing drugs. Beneficial effects

[0013] 1. This invention utilizes a ternary composite of Rhodiola rosea extract, mannose, and xanthan gum. Xanthan gum and mannose are not merely simple support and protectants; they exhibit a synergistic effect with Rhodiola rosea extract, achieving an activity enhancement effect greater than the sum of its parts (1+1+1>3). Experimental data demonstrate that the highly active Rhodiola rosea extract freeze-dried powder of this invention exhibits significantly enhanced anti-inflammatory activity while maintaining good analgesic activity.

[0014] 2. The freeze-drying process in this invention greatly improves the long-term storage stability of unstable components such as rhodioloside in Rhodiola rosea extract. The three-dimensional network structure formed by xanthan gum during freeze-drying can better "fix" and "encapsulate" active molecules, reduce their contact with oxygen and moisture, further slow down degradation, and improve stability.

[0015] 3. The freeze-dried powder of this invention has a porous and loose structure with excellent solubility. Xanthan gum, as a hydrophilic colloid, may prolong the retention time of active ingredients at the absorption site through adhesion, potentially improving bioavailability. Mannose itself has health benefits such as prebiotics, anti-inflammation, and anti-urinary tract infection, which complements the efficacy of Rhodiola rosea, resulting in multiple auxiliary effects and giving the product a richer health connotation.

[0016] 4. The preparation method of this invention is simple and mild, does not involve violent processes such as high temperature and high pressure, is easy to scale up and has high commercial value.

[0017] Instruction manual illustrations

[0018] Figure 1 Electron micrographs of the highly active Rhodiola rosea extract freeze-dried powder, mannose raw material, xanthan gum raw material, and physical mixture of mannose raw material and xanthan gum raw material (78:2) prepared in Example 2; Figure 2 Figure 1 shows the HPLC chromatograms, where Figure (A) is the HPLC chromatogram of the components of Rhodiola rosea extract; Figure (B) is the HPLC chromatogram of the components of the lyophilized powder of highly active Rhodiola rosea extract prepared in Example 2. Figure 3 A comparative graph showing the anti-inflammatory activity data of Rhodiola rosea extract and the highly active Rhodiola rosea extract lyophilized powder prepared in Example 2; Figure 4 A comparison graph showing the anti-inflammatory activity of Rhodiola rosea extract and the highly active Rhodiola rosea extract freeze-dried powder prepared in Example 2 against zebrafish. Figure 5 A comparison chart of the analgesic activity of Rhodiola rosea extract and the highly active Rhodiola rosea extract lyophilized powder prepared in Example 2. Detailed Implementation

[0019] This invention proposes a highly active Rhodiola rosea extract freeze-dried powder, its preparation method, and its applications. To make the objectives, technical solutions, and effects of this invention clearer and more explicit, the following will provide a more detailed description of the invention with reference to specific embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and are not intended to limit the invention.

[0020] Example 1 The extraction method for Rhodiola rosea extract is as follows: (1) Take dried Rhodiola rosea, grind it to 200 mesh to obtain Rhodiola rosea fine powder; (2) Soak Rhodiola rosea powder in 70% ethanol at a mass-volume ratio of 1g:10mL overnight; (3) Stir at 60℃ and 150rpm for 8 hours, then filter to obtain the extract; (4) Concentrate under pressure at a vacuum of 0.07 MPa and a temperature of 55°C until the solid content is 70%, and then dry to obtain the product.

[0021] Example 2 A method for preparing a highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix 4g of mannose and 2g of xanthan gum, add 12mL of water, stir until semi-dissolved, homogenize at 10000rpm for 10min to form a viscous transparent colloid, add water to 500mL, add 74g of mannose while stirring, stir until dissolved, slowly add 20g of Rhodiola rosea extract, stir at 500rpm for 30min, homogenize at 10000rpm for 15min to obtain a mixture; S2. Freeze-drying: Dispense the mixture into trays and pre-freeze at -40℃ for 5 hours. Then place it in a freeze dryer for freeze-drying under the following conditions: First stage drying at -10℃ for 20 hours under a vacuum of less than 20Pa, followed by second stage desorption drying at 20℃ for 6 hours to obtain freeze-dried blocks. S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0022] Example 3 A method for preparing a highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix 8g of mannose and 2g of xanthan gum, add 12mL of water, stir until semi-dissolved, homogenize at 10000rpm for 10min to form a viscous transparent colloid, add water to 500mL, add 81g of mannose while stirring, stir until dissolved, slowly add 20g of Rhodiola rosea extract, stir at 500rpm for 30min, homogenize at 10000rpm for 15min to obtain a mixture; S2. Freeze-drying: Dispense the mixture into trays and pre-freeze at -40℃ for 5 hours. Then place it in a freeze dryer for freeze-drying under the following conditions: First stage drying at -10℃ for 20 hours under a vacuum of less than 20Pa, followed by second stage desorption drying at 20℃ for 6 hours to obtain freeze-dried blocks. S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0023] Example 4 A method for preparing a highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix 4g of mannose and 2g of xanthan gum, add 12mL of water, stir until semi-dissolved, homogenize at 10000rpm for 10min to form a viscous transparent colloid, add water to 500mL, add 74g of mannose while stirring, stir until dissolved, slowly add 15g of Rhodiola rosea extract, stir at 500rpm for 30min, homogenize at 10000rpm for 15min to obtain a mixture; S2. Freeze-drying: Dispense the mixture into trays and pre-freeze at -40℃ for 5 hours. Then place it in a freeze dryer for freeze-drying under the following conditions: First stage drying at -10℃ for 20 hours under a vacuum of less than 20Pa, followed by second stage desorption drying at 20℃ for 6 hours to obtain freeze-dried blocks. S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0024] Example 5 A method for preparing a highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix 4g of mannose and 1g of xanthan gum, add 12mL of water, stir until semi-dissolved, homogenize at 10000rpm for 10min to form a viscous transparent colloid, add water to 500mL, add 74g of mannose while stirring, stir until dissolved, slowly add 20g of Rhodiola rosea extract, stir at 500rpm for 30min, homogenize at 10000rpm for 15min to obtain a mixture; S2. Freeze-drying: Dispense the mixture into trays and pre-freeze at -40℃ for 5 hours. Then place it in a freeze dryer for freeze-drying under the following conditions: First stage drying at -10℃ for 20 hours under a vacuum of less than 20Pa, followed by second stage desorption drying at 20℃ for 6 hours to obtain freeze-dried blocks. S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0025] Example 6 A method for preparing a highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix 4g of mannose and 1.5g of xanthan gum, add 12mL of water, stir until semi-dissolved, homogenize at 10000rpm for 10min to form a viscous transparent colloid, add water to 500mL, add 74g of mannose while stirring, stir until dissolved, slowly add 20g of Rhodiola rosea extract, stir at 500rpm for 30min, homogenize at 10000rpm for 15min to obtain a mixture; S2. Freeze-drying: Dispense the mixture into trays and pre-freeze at -40℃ for 5 hours. Then place it in a freeze dryer for freeze-drying under the following conditions: First stage drying at -10℃ for 20 hours under a vacuum of less than 20Pa, followed by second stage desorption drying at 20℃ for 6 hours to obtain freeze-dried blocks. S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0026] Example 7 A method for preparing a highly active Rhodiola rosea extract freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix 4g mannose and 2g xanthan gum, add 12mL water, stir until semi-dissolved, homogenize at 10000rpm for 10min to form a viscous transparent colloid, add water to 500mL, add 74g mannose while stirring, stir until dissolved, slowly add 20g Rhodiola rosea extract, stir at 500rpm for 30min, homogenize at 10000rpm for 15min, add 5g vitamin C and 3g maltodextrin, continue stirring until homogeneous to obtain a mixture; S2. Freeze-drying: Dispense the mixture into trays and pre-freeze at -40℃ for 5 hours. Then place it in a freeze dryer for freeze-drying under the following conditions: First stage drying at -10℃ for 20 hours under a vacuum of less than 20Pa, followed by second stage desorption drying at 20℃ for 6 hours to obtain freeze-dried blocks. S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

[0027] Comparative Example 1 The difference between this comparative example and Example 2 is that the amount of xanthan gum added is 0.5g.

[0028] Comparative Example 2 The difference between this comparative example and Example 2 is that xanthan gum is not added.

[0029] Comparative Example 3 The difference between this comparative example and Example 2 is that xanthan gum is replaced with hydroxypropyl methylcellulose.

[0030] Comparative Example 4 The difference between this comparative example and Example 2 is that mannose is replaced with hydroxypropyl-β-cyclodextrin.

[0031] Comparative Example 5 The difference between this comparative example and Example 2 is that mannose was replaced with maltodextrin (DE value 19).

[0032] Comparative Example 6 The difference between this comparative example and Example 2 is that no mannose was added.

[0033] Performance testing: 1. Basic antioxidant capacity test: Determination of DPPH free radical scavenging rate: the detection concentration was 2 μg / mL, the reaction time was 30 min, and the wavelength was 517 nm; Determination of ABTS free radical scavenging rate: detection concentration was 2 μg / mL, reaction time was 15 min, and wavelength was 734 nm; Determination of total reducing power: expressed as absorbance value, detection concentration was 2 μg / mL, and wavelength after reaction was 700 nm; All data are the average of three parallel experiments, and the results are shown in Table 1 below: Table 1 Antioxidant properties

[0034] As can be seen from Table 1 above, Example 2 has better overall antioxidant properties (lower than Example 7, possibly due to the addition of vitamin C), and the "Rhodiola rosea extract-xanthan gum-mannose" ternary system has a synergistic antioxidant effect.

[0035] 2. The highly active Rhodiola rosea extract freeze-dried powder prepared in Example 2 above was placed under an electron microscope and observed with mannose raw material, xanthan gum raw material, and a physical mixture of mannose raw material and xanthan gum raw material (78:2).

[0036] The results are as follows Figure 1 As shown, the freeze-dried powder formed a finer micron-sized morphology under an electron microscope; while mannitol and xanthan gum and their physical mixtures formed a larger blocky structure, indicating that the method formed a good micron-sized dispersion system.

[0037] 3. The components of the Rhodiola rosea extract and the highly active Rhodiola rosea extract lyophilized powder prepared in Example 2 were compared by HPLC. Figure 2 As shown.

[0038] 4. Comparison of the anti-inflammatory activity of Rhodiola rosea extract with that of highly active Rhodiola rosea extract freeze-dried powder prepared in Examples 2 and Comparative Examples 1-6. Zebrafish 72 hours post-fertilization (72 hpf) and in good developmental condition were selected, with 15 fish per well and three replicates per group. Experimental groups included a blank control group, a model group, an experimental group, and a positive control group. First, the buffer solution in the wells was aspirated. 1 mL of buffer solution was added to the blank control group and the model group, 1 mL of a solution containing different concentrations of the test compound was added to the experimental group, and 1 mL of the corresponding concentration of the positive control drug was added to the positive control group. The culture plates were pre-cultured in a 28.5℃ incubator for 2 hours. After 2 hours, the culture plates were removed, and each well was washed twice with Holt's buffer. Subsequently, 1 mL of Holt's buffer was added to the blank control group, and 1 mL of 20 μM copper sulfate solution (to induce an inflammatory response) was added to each well of the model group, each experimental group, and the positive control group. The culture plates were then placed back in the incubator for another 2 hours. After the incubation, the cells were observed under a microscope, images were acquired, and the neutrophil migration inhibition rate was calculated as: [1 - (experimental group - blank group) / (model group - blank group)] × 100%. The results are shown in Table 2 below. Table 2 Anti-inflammatory effects of Rhodiola rosea freeze-dried powder

[0039] As shown in Table 2 above and Figure 3 and Figure 4 As shown, at the same concentration, the lyophilized powder prepared in Example 2 (containing only 20% Rhodiola rosea extract) exhibited a significantly higher neutrophil migration inhibition rate than 100% Rhodiola rosea extract.

[0040] Synergy Factor (SC) Calculation: Actual inhibition rate: as in Example 2, it was 38.7 ± 20.7% at 0.25 μg / mL; Theoretical superposition inhibition rate: The single-component inhibition rate is weighted and superimposed according to the proportion of each component in the sample; Synergy coefficient (SC) determination criteria: SC > 1.2 is "significant synergy", 1.0 < SC ≤ 1.2 is "slight synergy", and SC ≤ 1.0 is "no synergy / antagonism".

[0041] Calculation formula: Example 2 (0.25 μg / mL) Actual inhibition rate (%) = 38.7% Theoretical superposition inhibition rate (%) = (26.5 × 20%) + (5.2 × 2%) + (3.1 × 78%) = 5.3 + 0.104 + 2.418 = 7.822% Synergy factor (SC) = 38.7% ÷ 7.822% ≈ 4.95; Example 2 (0.5 μg / mL) Actual inhibition rate (%) = 47.0% Theoretical superposition inhibition rate (%) = (32.4 × 20%) + (6.1 × 2%) + (3.8 × 78%) = 6.48 + 0.122 + 2.964 = 9.566% Synergy Factor (SC) = 47.0% ÷ 9.566% ≈ 4.91 As can be seen, Example 2 showed an anti-inflammatory inhibition rate of 38.7±20.7% at a concentration of 0.25 μg / mL, while the 100% Rhodiola rosea extract at the same concentration only showed 26.5±18.1%. The synergistic effect of the "Rhodiola rosea extract-xanthan gum-mannose" ternary system can be precisely quantified by the synergistic coefficient (SC≈4.95) – the activity is nearly 5 times higher than that of the single components, and this synergistic effect is concentration-dependent (SC≈4.91 at 0.5 μg / mL). This proves that xanthan gum is not just a carrier, but enhances the blocking ability of inflammatory signaling pathways through other means. At the same time, mannose stabilizes the structure of the complex, achieving a "significant synergy" of "1+1+1>3".

[0042] 5. Selected healthy, morphologically uniform 24hpf zebrafish embryos and randomly divided them into a blank control group, a model group, an experimental group, and a positive control group. Each group had three replicates, with five embryos placed in each well of a 96-well plate. First, remove the existing buffer solution from each well. Add 200 μL of Holt's buffer to each well in the blank control and model groups; add 200 μL of the test sample solution (7 μg / mL) to each well in the experimental group; and add 200 μL of 2 mM dipotassium glycyrrhizate solution to the positive control group. Incubate the plate at 28.5℃ for 1 hour. After incubation, remove the liquid from the wells. Add 200 μL of Holt's buffer to the blank control group; add 200 μL of 500 μM SDS solution to the model group; add 200 μL of 500 μM SDS solution containing 7 μg / mL of the test sample drug to the experimental group; and add 200 μL of 500 μM SDS solution containing 2 mM dipotassium glycyrrhizate to the positive control group. The interval between drug administrations was controlled at 30 seconds to ensure consistent treatment sequence. Recording began 15 minutes after treatment, with 30 seconds of video recorded per well, capturing the number of spontaneous embryonic turning movements. The average number of turning movements per well was calculated to assess the soothing effect of the test substance: fewer turning movements indicated a stronger soothing effect. The inhibition rate was calculated as: [1 - (Experimental group - Blank group) / (Model group - Blank group)] × 100%. The results are shown in Table 3. Table 3 Analgesic activity of Rhodiola rosea freeze-dried powder

[0043] As shown in Table 3 above and Figure 5As shown, the freeze-dried powder prepared in Example 2 at the same concentration (the content of Rhodiola rosea extract in the freeze-dried powder is only 20%) has a lower activity ratio than Rhodiola rosea extract. However, since the freeze-dried powder system contains only 20% Rhodiola rosea extract, the effect is still better than that of Rhodiola rosea extract. The system maintains good activity and also has a synergistic effect in analgesic activity.

[0044] Table 3 shows that the Rhodiola rosea extract content in the freeze-dried powder of Example 2 was only 20%, but at a concentration of 10 μg / mL, the analgesic inhibition rate reached 41.9±6.2%, which, after conversion based on content, is equivalent to 209.5% (41.9%÷20%) of 100% Rhodiola rosea extract, significantly higher than 88.1±2.2% when Rhodiola rosea extract was used alone. This indicates that the ternary system, while maintaining analgesic activity, significantly improves the utilization efficiency of Rhodiola rosea extract. The hydrophilic colloidal properties of xanthan gum prolong the retention time of the active ingredient at the painful site, and mannose helps to reduce the local inflammatory microenvironment. The two work together to enhance the analgesic effect, solving the pain point of "low utilization rate of active ingredient" in traditional formulations.

[0045] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A highly active Rhodiola rosea extract freeze-dried powder, characterized in that: Composed of the following ingredients, in parts by weight: Rhodiola rosea extract: 10-20 parts; Mannose: 78-89 parts; Xanthan gum: 1-2 parts; Other pharmaceutically acceptable excipients: 0-10 parts; The extraction method of the Rhodiola rosea extract is as follows: (1) Take dried Rhodiola rosea, grind it to 200 mesh to obtain Rhodiola rosea fine powder; (2) Soak Rhodiola rosea powder in 70% ethanol at a mass-volume ratio of 1g:10mL overnight; (3) Stir at 60℃ and 150-200rpm for 8 hours, then filter to obtain the extract; (4) Concentrate under pressure at a vacuum of 0.06-0.08 MPa and a temperature of 50-60℃ until the solid content is ≥50%, and then dry to obtain the product; The method for preparing the freeze-dried powder includes the following steps: S1. Dissolving and mixing: Mix part of the mannose and xanthan gum, add an appropriate amount of water, stir until semi-dissolved, homogenize at high speed for the first time, add water to 5 times the total volume, add the remaining mannose while stirring, stir until dissolved, slowly add Rhodiola rosea extract, stir evenly, homogenize at high speed for the second time to obtain the mixture. S2. Freeze-drying: Pre-freeze the mixture at -40℃ or below for 4-6 hours, then freeze-dry until the moisture content is ≤5% to obtain freeze-dried blocks; S3. Crushing and Packaging: Crush the freeze-dried blocks and pass them through a 200-mesh sieve to obtain freeze-dried powder.

2. The highly active Rhodiola rosea extract freeze-dried powder according to claim 1, characterized in that: Composed of the following ingredients, in parts by weight: Rhodiola rosea extract: 20 parts; Mannose: 78 parts; Xanthan gum: 2 parts.

3. The highly active Rhodiola rosea extract freeze-dried powder according to claim 1, characterized in that: Other pharmaceutically acceptable excipients include vitamin C and maltodextrin.

4. The highly active Rhodiola rosea extract freeze-dried powder according to claim 1, characterized in that, The first high-speed homogenization condition in S1 is 10,000 rpm for 10-12 min; the second high-speed homogenization condition is 10,000 rpm for 10-15 min.

5. The highly active Rhodiola rosea extract freeze-dried powder according to claim 1, characterized in that, The stirring speed in S1 is 500-600 rpm.

6. The highly active Rhodiola rosea extract freeze-dried powder according to claim 1, characterized in that, The freeze-drying conditions in S2 are as follows: under a vacuum degree of less than 20 Pa, the first stage of drying is carried out at -20℃ to 0℃ for 10-20 hours, followed by the second stage of desorption drying at 20-30℃ for 4-8 hours.

7. The use of the highly active Rhodiola rosea extract freeze-dried powder according to any one of claims 1-6 in the preparation of antioxidant, anti-inflammatory, analgesic or immune-enhancing drugs.

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