Insecticidal composition and application thereof

Abstract

The invention discloses an insecticidal composition and application thereof, and belongs to the technical field of pesticides. The effective components of the insecticidal composition are the isoxaflutole and the pyridalyl, and the mass ratio of the isoxaflutole to the pyridalyl is (1: 80)-(80: 1). The insecticidal composition provided by the invention is formed by compounding the effective components isoxtolfenpyrad and pyridalyl with different action mechanisms, and the two components complement each other in action and show a remarkable synergistic effect instead of simple activity superposition; the composition can effectively reduce the pesticide application frequency and the pesticide application cost, is beneficial to improvement of farmer income, reduces pesticide residues and environmental pollution, is beneficial to overcoming and delaying development of pest resistance to drugs, and has good safety to crops.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, and in particular relates to an insecticidal composition and its application. Background Technology

[0002] Leaf-eating pests are a group of agricultural and forestry pests that cause damage by feeding on plant leaf tissue during their larval stage. They belong to different taxa such as Lepidoptera, Coleoptera, and Hymenoptera. Their larvae are voracious eaters; early instars feed on the leaf mesophyll, creating window-like damage; 2nd and 3rd instars cause leaf notches; and after the 4th instar, they can devour the entire leaf, leaving only the veins. Adults generally have the ability to migrate and spread, and larvae expand their damage range through swarm migration, exhibiting periodic outbreaks under suitable climates. Typical species include the tussock moth, the tussock moth, and the tussock leafminer. The tussock moth, in particular, has been recorded causing damage in Dingbian County with a maximum of 4 larvae per plant and an average of 67 larvae per 100 plants. Control measures mainly employ a comprehensive approach combining aerial spraying with biomimetic agents, chemical spraying, and physical barriers.

[0003] With the widespread adoption and extensive use of pesticides, single-active-component insecticides have become increasingly problematic in agricultural pest control. These drawbacks include narrow pest coverage, poor efficacy, high dosage leading to resistance and phytotoxicity, frequent application, and increased environmental pollution. These issues have resulted in significant crop yield reductions and substantial increases in production costs. One approach to controlling resistant pests is to introduce new active ingredients that do not exhibit cross-resistance with existing varieties. However, developing new active ingredients is costly, time-consuming, and cannot keep pace with the rate at which pests develop resistance. Other methods, such as adjusting crop rotation patterns and rotating different pesticides, are often ineffective in practice.

[0004] Compared to the long development cycle of new pesticides, the development of highly efficient and low-toxicity pesticide compound compositions has the advantages of low investment, short cycle and easy promotion, and therefore continues to receive attention from pesticide researchers at home and abroad. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the technical problem to be solved by this invention is that single-active-component insecticides have poor control effects in agricultural insecticide application, high dosage, and are prone to resistance. This invention proposes an insecticidal composition containing isoxaflutole and trifluralin and its application, and develops it into a compound insecticide with significant synergistic effects, low cost, good efficacy, long-lasting effect, and low environmental pollution.

[0006] To solve the aforementioned technical problem, the technical solution adopted by the present invention is as follows: In one aspect, the present invention provides an insecticidal composition, the active ingredients of which are isoxazoline and trifluralin, wherein the mass ratio of isoxazoline to trifluralin is 1:80 to 80:1.

[0007] Isoxazolamide is a broad-spectrum contact insecticide intended for the control of pests in agricultural crops, lawns, and ornamental plants, as well as indoor and outdoor pest control in commercial, industrial, and household environments. Its chemical name is: 4-[(5RS)-5-(3,5-dichloro-4-fluorophenyl)-4,5-dihydro-5-(trifluoromethyl)isozol-3-yl]-N-[(4RS)-2-ethyl-3-oxoisozolidine-4-yl]-o-toluamide; molecular formula: C 23 H 19 Cl2F4N3O4; relative molecular mass: 548.314. Target pests of isoxazoline include the brown stink bug (primarily damaging cotton), the Colorado potato beetle (primarily damaging potatoes), and the diamondback moth (primarily damaging cruciferous vegetables), which are serious threats to crops. Isoxazoline is a non-competitive inhibitor of the γ-aminobutyric acid (GABA) receptor and is classified as a Group 30 (Group 1) GABACl allosteric regulator by the International Resistant Insecticide (IRAC) Committee. The structure of isoxazoline is similar to that of fluxametamide and may share the same binding site with fluralaner.

[0008] Trifluoromethylpyrimidine, also known as acetamiprid, is a highly effective and low-toxicity insecticide developed by Sumitomo Chemical Co., Ltd. of Japan. It is primarily used to control lepidopteran larvae. Its chemical name is: 2-{3-[2,3-dichloro-4-(3,3-dichloro-2-propenyloxy)phenoxy]propoxy}-5-(trifluoromethyl)pyrimidine; molecular formula: C 18 H 14 Cl4F3NO3; relative molecular mass: 491.1159. The technical grade is a yellow liquid with a boiling point of 227℃ (decomposes), is pH stable, and is readily soluble in most organic solvents. This agent primarily acts as a stomach poison and ovicidal agent, achieving its insecticidal effect by interfering with insect nerve signal transmission, impairing muscle cell function, and causing abnormal molting. It exhibits no cross-resistance with existing insecticides. Trifluralin has a unique chemical structure and a different mechanism of action than commonly used pesticides, and is mainly used to control lepidopteran larvae that damage crops.

[0009] The applicant used isoxaflutole and trifluralin in combination and found that leaf-eating pests, after being treated, initially became slow-moving, and soon showed initial symptoms, becoming completely paralyzed and immobile within 1 hour. The effect was significantly better than either of the two single agents / components, and the combination distributed slowly and evenly on the leaves, effectively and for a long time protecting plant leaves from pest infestation. This combination can be used to control leaf-eating pests. There are no existing reports or studies on the combination of these two agents in the prior art.

[0010] Preferably, the mass ratio of isoxazoline to trifluralin is 1:20 to 20:1.

[0011] Preferably, the mass ratio of isoxazoline to trifluralin is 1:10 to 10:1.

[0012] It is understood that the mass ratio of isoxazolam to trifluralin can be 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, or any value within the range thereof.

[0013] Preferably, the total mass of the isoxazoline and the trifluralin accounts for 1% to 90% of the mass of the insecticidal composition.

[0014] Preferably, the total mass of the isoxazoline and the trifluralin accounts for 10% to 60% of the mass of the insecticidal composition.

[0015] It is understood that the content of the active ingredient in the composition of the present invention depends on the application amount when used alone, the mixing ratio of one compound with another, the degree of synergistic effect, and also on the target disease. Typically, the weight percentage of the active ingredient in the composition is 1% to 90% of the total weight, preferably 5% to 80%. The range of active ingredient content varies depending on the type of formulation. Generally, liquid formulations contain 1% to 60% of the active substance by weight, preferably 5% to 50%; solid formulations contain 5% to 80% of the active substance by weight, preferably 10% to 80%.

[0016] Preferably, the insecticidal composition further includes agricultural formulation adjuvants, which include carriers, solvents, auxiliaries, and fillers.

[0017] Preferably, the carrier includes one or more mixtures of clay particles, crushed brick particles, sand, plant-derived carriers, fertilizers, etc. The solvents include one or more of the following: various solvent oils, organic solvents such as N-methylpyrrolidone, acetophenone, cyclohexanone, and dimethyl sulfoxide, as well as plant-derived environmentally friendly solvents such as turpentine, methyl oleate, soybean oil, corn oil, and rapeseed oil.

[0018] The filler is selected from one or more of the following: kaolin, diatomaceous earth, bentonite, attapulgite, silica, starch, or light calcium carbonate.

[0019] The adjuvant includes at least one surfactant, and the surfactant content accounts for 2% to 30% of the total weight of the insecticidal composition. Depending on the application and requirements, other functional adjuvants such as antifreeze, thickener, stabilizer, disintegrant, defoamer, warning color, and film-forming agent may also be added.

[0020] The surfactant is selected from one or more of emulsifiers, dispersants, wetting agents, or penetrants. The surfactants described above are common nonionic or anionic surfactants, either as single agents or in combination.

[0021] The emulsifiers are selected from Agricultural Emulsion 500# (calcium dodecylbenzenesulfonate), tristyrene phenol polyoxyethylene ether, OP series phosphate esters (nonylphenol polyoxyethylene ether phosphate ester), 600# phosphate ester (phenylphenol polyoxyethylene ether phosphate ester), styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether magnesium disulfonate, triethanolamine salt, Agricultural Emulsion 400# (benzyl dimethylphenol polyoxyethylene ether), Agricultural Emulsion 700# (alkylphenol formaldehyde resin polyoxyethylene ether), and Ningxia Emulsion 36# (phenylethylphenol formaldehyde). The mixture consists of one or more of the following: resin polyoxyethylene ether, agricultural emulsion 1600# (phenylethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agricultural emulsion 33# (alkyl aryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (dehydrated sorbitan fatty acid ester polyoxyethylene ether), or AEO series (fatty alcohol polyoxyethylene ether).

[0022] The dispersant is selected from one or more of the following: polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, sodium naphthalene sulfonate formaldehyde condensate, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, or glycerol fatty acid ester polyoxyethylene ether.

[0023] The wetting agent is selected from one or more of the following: sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, pull-opening powder BX, wetting and penetrating agent F, soapberry powder, silkworm excrement, or soapberry powder.

[0024] The penetrant is selected from one or more of the following: penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone, or organosilicon.

[0025] The antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea, or a mixture thereof.

[0026] The thickener is selected from one or more of the following: gelatin, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, xanthan gum, bentonite, silica, or magnesium aluminum silicate.

[0027] The stabilizer is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, or triphenyl phosphate.

[0028] The disintegrant is selected from one or more of the following: bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate.

[0029] The defoamer is selected from one or more of the following: silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds, or C8-C10 fatty alcohol compounds.

[0030] All of the substances mentioned above are available on the market.

[0031] The insecticidal composition of the present invention can be diluted or used directly by the user before use. Its formulation can be prepared by processing methods known to those skilled in the art, namely, mixing the active ingredient with a liquid solvent or solid carrier, and then adding one or more surfactants such as dispersants, stabilizers, wetting agents, binders, and defoamers.

[0032] The insecticidal composition of the present invention can be processed into any pesticide-acceptable formulation as needed. Preferred formulations include wettable powders, water-dispersible granules, pellets, suspensions, emulsions, microemulsions, water-in-oil emulsions, dispersible oil suspensions, seed-treated dispersible powders, or seed-treated suspensions.

[0033] When the composition is formulated into a wettable powder, it contains the following components and amounts:

[0034] When the composition is formulated into a seed treatment dispersible powder, it contains the following components and amounts:

[0035] When the composition is formulated into water-dispersible granules, it includes the following components and their contents:

[0036] When the composition is formulated into a suspension, it includes the following components and their amounts:

[0037] When the composition is formulated into a seed treatment suspension, it includes the following components and their amounts:

[0038] When the composition is formulated into a suspension emulsion, it includes the following components and their amounts:

[0039] When the composition is formulated into a soluble concentrate, it includes the following components in the following amounts:

[0040] When the composition is formulated into an aqueous emulsion, it includes the following components and their amounts:

[0041] When the composition is formulated into a microemulsion, it includes the following components and their amounts:

[0042] When the composition is formulated into a dispersible oil suspension, it includes the following components and their amounts:

[0043] When the composition is formulated into granules, it includes the following components and their amounts:

[0044] The main technical indicators of the various formulations of this invention all meet the requirements of relevant standards such as the "Specifications for the Formulation of Product Specifications for Pesticide Registration".

[0045] In another aspect, the present invention provides the application of the above-mentioned insecticidal composition in the control of leaf-eating pests, wherein the active ingredients isoxazoline and trifluralin in the insecticidal composition synergistically promote insecticidal action.

[0046] Preferably, the leaf-eating pests are leaf-eating pests on vegetables, cotton, fruit trees, corn, tobacco, flowers, melons, or legumes.

[0047] Compared with the prior art, the beneficial effects of the present invention are as follows: The insecticidal composition provided by this invention is a combination of isoxaflutole and trifluralin, which are active ingredients with different mechanisms of action. The two components have complementary effects and exhibit a significant synergistic effect, rather than a simple additive effect. This composition can effectively reduce the number of applications and the cost of pesticides, which helps to increase farmers' income. At the same time, it reduces pesticide residues and environmental pollution. In addition, it helps to overcome and delay the development of pesticide resistance in pests and has good safety for crops. Detailed Implementation The technical solutions in specific embodiments of the present invention will be described in detail and completely below. Obviously, the described embodiments are only some specific implementations of the overall technical solution of the present invention, and not all implementations. Based on the overall concept of the present invention, all other embodiments obtained by those skilled in the art fall within the protection scope of the present invention.

[0048] The efficacy of isoxaflutole and trifluralin in different combinations against leaf-eating pests in southern regions was tested. The experimental method is as follows: This invention employs a method combining indoor toxicity testing and field trials. First, indoor toxicity testing is used to determine the co-toxicity coefficient (CTC) of two agents mixed in a specific ratio. A CTC < 80 indicates antagonistic effects, 80 ≤ CTC ≤ 120 indicates additive effects, and CTC > 120 indicates synergistic effects. Based on this, field trials are then conducted.

[0049] Experimental method: During the experiment, the aqueous suspension stock solution of each mixture was diluted to five series of concentrations and placed in beakers for later use.

[0050] Culture of leaf-eating pests: Take a large number of cabbage leaves, rinse them gently with water, and use a dissecting needle to gently pick out the milky white egg sacs on the diseased leaves. Place them in a small petri dish with a diameter of 6cm, add a small amount of sterile water, and incubate them in a constant temperature incubator at 25℃ for 3-4 days. Follow Xu Jinjun's method throughout the process. Collect the second instar larvae and add sterile water to prepare a suspension of a certain concentration (about 1000 larvae / mL) for later use.

[0051] The 96-well plate method was used. A suspension of leaf-eating pests was added to each well using a continuous pipette, 30 μL per well. Different dosages of pesticide solution were then added in ascending order, resulting in five different concentration gradients. The plates were shaken well and incubated at 25°C with 60-80% humidity for observation. Each treatment was replicated in triplicate, with a water control included. Insects were considered dead if they were stiff and immobile, and alive if they were flexing or wriggling. Mortality rates were observed at 24h, 48h, and 72h. Mortality rates and corrected mortality rates were calculated. Using Finney probability analysis, the corrected mortality rate was converted to a probability value (y), and the treatment concentration (μg / ml) was converted to a logarithmic value (x). Data processing was performed using IBM SPSS Statistics software to derive toxicity regression equations, from which the LC50 of each pesticide was calculated. 50 Value. If the mortality rate in the control group is greater than 10%, the trial is considered invalid. The calculation formula is as follows: Mortality rate (%) = (Number of live worms before treatment - Number of live worms after treatment) / Number of live worms before treatment × 100%; Mortality correction rate (%) = (treatment group mortality rate - control group mortality rate) / (100 - control group mortality rate) × 100%; The co-toxicity coefficient (CTC) was calculated using Sun Yunpei's formula. The calculation formula is as follows (using isoxaflutole as the standard agent, with a toxicity index of 100): The toxicity index (TI) of trifluralin = (LC50 of isoxazolidin) 50 LC of trifluoperylate50 ) ×100; Actual Toxicity Index (ATI) of M = (LC50 of isoxazolidinyl) 50 / M of LC 50 ) × 100; Theoretical toxicity index (TTI) of M = TI of isoxazolamide × P 异噁唑虫酰胺 +Effective trifluoromethylpyridine TI×P 三氟甲吡醚 ; Cotoxicity coefficient (CTC) = (M's ATI / M's TTI) × 100 In the formula: M represents mixtures with different proportions; P 异噁唑虫酰胺 The percentage of isoxazoline in the composition; P 三氟甲吡醚 This represents the proportion of trifluoromethylpyridine in the composition.

[0052] The toxicity test results are shown in Table 1, and the synergistic effect test results are shown in Table 2. CK1 represents isoxaflutole as a single agent, and CK2 represents trifluralin as a single agent.

[0053] Table 1. Results of toxicity tests on leaf-eating pests of Chinese cabbage using different ratios of isoxazoline and trifluralin.

[0054] Table 2. Results of synergistic effects of different ratios of isoxazoline and trifluralin on leaf-eating pests of Chinese cabbage.

[0055] Tables 1 and 2 show that the co-toxicity coefficients of the isoxaflutole and trifluralin combinations are all greater than 100, indicating a synergistic effect. The synergistic effect is most pronounced at weight ratios of 1:1 and 1:2:1:5:1:10. This demonstrates that the application of isoxaflutole and trifluralin compound formulations with weight ratios of 1:1–10 for the control of leaf-eating pests on cabbage is reasonable and feasible.

[0056] The above methods were used to determine the control effects of isoxaflutole on leaf-eating pests of tomatoes, peppers, and cucumbers, and the synergistic coefficients within different ratio ranges were calculated. The results showed that the combination of isoxaflutole and trifluralin exhibited the most significant synergistic effect at ratios of 1:1, 1:2:1:5:1:10.

[0057] The present invention will be described in detail below with reference to the embodiments. All percentages in the embodiments are weight percentages, but the present invention is not limited thereto. The treatment dosages for field efficacy are all dosages of the active ingredient.

[0058] The following are wettable powders: Example 1: (40% isoxaflutole·triflumethrin wettable powder) 1:3 Components and content (mass percentage): Isoxazolamide: 10%; Trifluralin: 30%; Wetting agent: Sodium dodecyl sulfate: 5%; Dispersant: Calcium lignosulfonate: 8%; Dispersant: Naphthalenesulfonate: 4%; Filler: Silica: 5%; Defoamer: 0.2%; Kaolin: Balance.

[0059] Example 2: (70% isoxaflutole·triflumethrin wettable powder) 1:6 Components and content (mass percentage): Isoxazolamide: 10%; Trifluralin: 60%; Wetting agent sodium dodecylbenzenesulfonate: 3%; Dispersant calcium alkylbenzenesulfonate: 7%; Dispersant naphthalenesulfonate: 3%; Filler selected: silica: 5%; Defoamer: 0.2%; Light calcium carbonate: balance.

[0060] Example 3: (11% isoxaflutole·triflumethrin wettable powder) 1:0.1 Components and content (mass percentage): Isoxazolamide: 10%; Trifluralin: 1%; Wetting agent: Sodium dodecylbenzenesulfonate and Wetting and Penetrating Agent F, with dosages of 1%, 1%, and 1% respectively; Dispersant: Polycarboxylate and Naphthalenesulfonate, with dosages of 1% and 3% respectively; Silica: 3%; Defoamer: 0.2%; Filler: Diatomaceous earth: 20%, Kaolin: Balance.

[0061] The preparation methods of Examples 1-3 above are as follows: According to the components and their weight percentages provided in the examples, the active ingredients trifluralin and isoxaflutole are added to the filler, along with wetting agents, dispersants, and other additives. The mixture is then homogenized, pulverized by air jet milling, and then further mixed to obtain a wettable powder. The main equipment includes a mixer and an air jet mill.

[0062] In the above embodiments, all indicators of the formulation samples meet the requirements of the wettable powder management specifications.

[0063] Water-dispersible granules are as follows: Example 4: (30% isoxazoline·triflumethrin water-dispersible granules) 1:2 Components and content (mass percentage): Isoxazolamide: 10%; Trifluralin: 20%; Wetting agent: Sodium dodecylbenzenesulfonate: 3%; Dispersant: Alkylnaphthalenesulfonate formaldehyde condensate: 8%; Defoamer: 0.2%; Filler: Attapulgite: Balance.

[0064] Example 5: (42% isoxazoline·triflumethrin water-dispersible granules) 1:5 Components and content (mass percentage): Isoxazolamide: 7%; Trifluralin: 35%; Wetting agent: soapberry powder: 3%; Dispersant: sodium naphthalenesulfonic acid formaldehyde condensate: 2%; Dispersant: polycarboxylate: 2%; Defoamer: 0.2%; Filler: attapulgite clay balance.

[0065] The preparation methods of Examples 4-5 above are as follows: According to the components and their weight percentages provided in the examples, the active ingredients trifluralin and isoxaflutole are added to the filler, along with wetting agents, dispersants, and other additives. The mixture is then air-jet pulverized, and 10-25% water is added. The mixture is then kneaded, granulated, dried, and sieved to obtain a water-dispersible granule product. Alternatively, the pulverized powder is sprayed with water, granulated, dried, and then sieved in a fluidized bed granulator to obtain the product. The main equipment includes a mixer, air-jet pulverizer, kneader, extrusion granulator, drying oven or fluidized bed dryer, or fluidized bed granulator and sieve.

[0066] In the above examples, all the formulation samples met the requirements of the regulations for the management of water-dispersible granules.

[0067] The suspending agent is as follows: Example 6: (27% isoxaflutole·triflumethrin suspension) 1:8 Components and content (mass percentage): Isoxazolidinamide: 3%; Trifluralin: 24%; Wetting agent NP-10: 2%; Dispersant polycarboxylate: 2%; Dispersant phosphate ester: 2%; Thickeners are xanthan gum and magnesium aluminum silicate, with dosages of 0.1% and 1% respectively; Antifreeze ethylene glycol: 5%; Defoamer silicone compound: 0.2%; Water, balance.

[0068] Example 7: (5.5% isoxaflutole·triflumethrin suspension) 1:10 Components and content (mass percentage): Isoxazolidinamide: 0.5%; Trifluralin: 5%; Wetting agent NP-10: 1%; Dispersant alkylphenol polyoxyethylene ether: 3%; Dispersant polycarboxylate: 2%; Thickeners are xanthan gum and magnesium aluminum silicate, with dosages of 0.15% and 2% respectively; Antifreeze agent ethylene glycol: 5%; Defoamer silicone oil: 0.2%; Water: balance.

[0069] Comparative Example 1: (30% trifluoperyl pyridine suspension) Components and content (mass percentage): Trifluoromethylpyridine 30%; Wetting agent sodium dodecyl sulfate: 2%; Dispersant polycarboxylate: 2%; Dispersant phosphate ester: 3%; Dispersant alkylphenol polyoxyethylene ether: 2%; Thickeners selected are xanthan gum and magnesium aluminum silicate, with dosages of 0.15% and 1.5% respectively; Antifreeze agent ethylene glycol: 5%; Defoamer silicone oil: 0.2%; Water: balance.

[0070] Comparative Example 2: (30% isoxazolam suspension) Components and content (mass percentage): Isoxazolamide: 30%; Wetting agent: sodium dodecyl sulfate: 2%; Dispersant: polycarboxylate: 2%; Dispersant: phosphate ester: 3%; Dispersant: polymer: 2%; Thickeners: xanthan gum and magnesium aluminum silicate, at 0.1% and 1% respectively; Antifreeze: ethylene glycol: 5%; Defoamer: silicone oil: 0.2%; Water: balance.

[0071] The preparation methods for Examples 6-7 and Comparative Examples 1 and 2 are as follows: According to the components and their weight percentages provided in the examples, the active ingredient trifluralin, isoxaflutole, wetting agent, dispersant, thickener, antifreeze, defoamer, and other additives are sequentially placed in a reaction vessel, water is added and mixed evenly, followed by high-speed shearing, wet milling, and finally homogenization filtration to obtain the product. The main equipment includes a batching vessel, a colloid mill or homogenizer, and a sand mill.

[0072] The above examples and comparative examples show that all indicators of the formulation samples meet the requirements of the suspension management regulations.

[0073] The following are dispersible oil suspensions: Example 8 (mass percentage): (30% isoxaflutole·triflumethrin dispersible oil suspension) 1:2 Composition and content: Isoxazolidinone: 10%, Trifluralin: 10%, Calcium dodecylbenzenesulfonate: 5%, Castor oil polyoxyethylene ether: 1%, Fatty alcohol polyoxyethylene ether: 8%, Thickeners selected are organo-earth and fumed silica, each 0.7%; Soybean oil balance.

[0074] Example 9 (mass percentage): (35% isoxaflutole·triflumethrin dispersible oil suspension) 3:4 Components and content: Isoxazolidinamide: 15%, Trifluralin: 20%, Calcium dodecylbenzenesulfonate: 4%, Castor oil polyoxyethylene ether: 2%, Fatty alcohol polyoxyethylene ether: 10%, Isomerized alcohol ether: 2%, Thickeners selected are organo-earth and fumed silica, each 0.6%; Soybean oil balance.

[0075] The preparation methods of Examples 8-9 above are as follows: Soybean oil, the active ingredient trifluralin, isoxaflutole, emulsifier, dispersant, thickener, defoamer, and other additives are sequentially placed in a reaction vessel according to the components and their weight percentages provided in the examples. The mixture is then thoroughly mixed, subjected to high-speed shearing, wet milling, and finally homogenized and filtered to obtain the product. The main equipment includes a batching vessel, a colloid mill or homogenizer, and a sand mill.

[0076] In the above embodiments, all indicators of the formulation samples meet the requirements of the regulations for the management of dispersible oil suspensions.

[0077] The following field drip irrigation experiments were conducted using pesticides from Examples 1, 4, 6, and 8, and pesticides from Comparative Examples 1 and 2, at the dilution ratios shown in Table 3, to more comprehensively and accurately illustrate the effects of the present invention. The experimental field of Qingdao Hansheng Biotechnology Co., Ltd. in Laixi City, Qingdao, was selected as the experimental site. During the experiment, soil conditions, water and fertilizer management, and other management practices were kept consistent. The experimental results are shown in Table 3 below.

[0078] Example 1: 40% isoxazoline·triflumethrin wettable powder (10+30). Example 4: 30% isoxaflutole·triflumethrin water-dispersible granules (10+20); Example 5: 42% isoxaflutole·triflumethrin water-dispersible granules (7+35); Example 6: 27% isoxaflutole·triflumethrin aqueous suspension (3+24); Example 8: 30% isoxaflutole·triflumethrin dispersible oil suspension (10+20); Comparative Example 1: 30% Trifluridine aqueous suspension; Comparative Example 2: 30% isoxazolam aqueous suspension.

[0079] This experiment included eight treatments: Examples 1, 4, 5, 6, and 8 (using pesticides), Comparative Examples 1 and 2 (using pesticides), and no pesticide used. Each treatment was repeated three times, resulting in a total of 24 treatment plots, each plot measuring 30 m. 2 Isolation rows were set up between each treatment area, and the treatments in each area were randomly arranged. The dosage of the active ingredient per plant was 0.01g, diluted in 400ml of solution, and applied via drip irrigation at the early stage of leaf-eating pest infestation in cabbage. Three points were randomly selected from each treatment area, with three cabbage plants taken from each point. The leaf condition was assessed before application and at 1, 3, and 7 days after application. The grading criteria for leaf-eating pest infestation were: Grade 0 (no visible pests on leaves), Grade 1 (1%-10% of leaves affected), Grade 2 (10%-25% of leaves affected), Grade 3 (25%-50% of leaves affected), Grade 4 (50%-80% of leaves affected), and Grade 5 (over 80% of leaves affected). The effectiveness in controlling leaf-eating pests was based on two indicators: the leaf-eating pest disease severity index and the control effect. The growth of cabbage in each area and the occurrence of pesticide damage were also observed and recorded.

[0080] Methods for calculating disease index and prevention and control effectiveness: Disease index (%) = [Σ(Number of plants at each disease level × Disease level) / (Total number of plants surveyed × Highest disease level)] × 100%; Prevention and control effect (%) = [(Disease index in control area - Disease index in treatment area) / Disease index in control area] × 100%; Table 3. Control effects of different treatments on leaf-eating pests in Chinese cabbage.

[0081] As shown in Table 3, after 1, 3, and 7 days of application, the pesticides in Examples 1, 4, 5, 6, and 8 were significantly more effective than the single-agent pesticides isoxaflutole and trifluralin in Comparative Examples 1-2 against leaf-eating pests of Chinese cabbage. Furthermore, the pesticides had a longer residual effect, maintaining good control even 7 days after application. Table 3 also indicates that, for formulations with the same ratio and content, the efficacy of oil-dispersible suspensions is higher than that of water-dispersible granules, verifying that the oil in the formulation promotes its efficacy. During the experiment, no phytotoxicity was observed in the pesticides used in each example, demonstrating good safety.

[0082] In addition, referring to the above treatment methods, the 30% isoxazoline·triflumethrin dispersible oil suspension OD (1:2) prepared in Example 8 was used to conduct a life test on three crops: tomato, pepper and cucumber. The effective ingredient dosage per plant was 0.01g, and 400ml of diluted solution was used for drip irrigation. The test data are shown in Table 4.

[0083] Table 4. Control efficacy of 30% isoxaflutole·triflumethrin OD against leaf-eating pests in tomatoes, peppers, and cucumbers.

[0084] Table 4 further verifies that the combination of isoxaflutole and trifluralin has a good control effect on leaf-eating pests of different crops such as tomatoes, peppers, and cucumbers. The control effect is the highest on the 3rd day and still has a high control ability on the 7th day. The effect is long-lasting. During the application period, crop production is normal and no obvious phytotoxicity occurs.

[0085] The agent (30% isoxaflutole·triflumethrin dispersible oil suspension OD prepared in Example 8) was continued to be used to target cucumber leaf-eating pests. 0.01g, 0.012g, and 0.015g of active ingredient were used respectively, and the solution was diluted 400m for drip irrigation to further verify the control effect of different dosages on leaf-eating pests. The experimental data are shown in Table 5.

[0086] Table 5. Effects of different OD dosages of 30% isoxaflutole·triflumethrin on the control of leaf-eating pests in cucumber.

[0087] As shown in Table 5, with the increase of the dosage, the control effect of 30% isoxaflutole·triflumethrin dispersible oil suspension OD on cucumber leaf-eating pests also showed an increasing trend, reaching its peak on the 3rd day, and still having a high control effect on the 7th day. The effect lasted for a long time, and the cucumber grew normally throughout the entire treatment period without any obvious phytotoxicity.

[0088] All the above results fully demonstrate that the combination of isoxaflutole and trifluralin has the advantages of significant synergistic effect and prolonged efficacy against leaf-eating pests, and is safe throughout the entire process.

Claims

1. An insecticidal composition, characterized by comprising, The effective components are isothiazolyl amide and triflumezopyr, and the mass ratio of the isothiazolyl amide to the triflumezopyr is 1:80-80:

1.

2. The insecticidal composition according to claim 1, characterized by, The mass ratio of the isothiazolyl amide to the triflumezopyr is 1:20-20:

1.

3. The insecticidal composition according to claim 2, wherein The mass ratio of the isothiazolyl amide to the triflumezopyr is 1:10-10:

1.

4. The insecticidal composition according to claim 1, wherein The total mass of the isothiazolyl amide and the triflumezopyr accounts for 1%-90% of the mass of the insecticidal composition, preferably 1%-90%.

5. The insecticidal composition according to claim 4, wherein The insecticidal composition further comprises an agricultural formulation auxiliary component, which comprises a carrier, a solvent, an auxiliary agent and a filler; The carrier comprises a mixture of one or more of clay particles, broken brick particles, sandy soil, plant-derived carriers, fertilizers and the like; The solvent comprises a mixture of one or more of various solvent oils, N-methyl pyrrolidone, phenylacetone, cyclohexanone, dimethyl sulfoxide and other organic solvents, and a mixture of pine oil, methyl oleate, soybean oil, corn oil, rapeseed oil and other plant-derived environmentally friendly solvents; The filler is selected from a mixture of one or more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate.

6. The insecticidal composition according to claim 5, wherein The auxiliary agent at least comprises a surfactant, and the content of the surfactant accounts for 2%-30% of the total weight of the insecticidal composition.

7. The insecticidal composition according to claim 6, wherein The auxiliary agent further comprises other functional auxiliary agents selected from one or more of antifreeze agents, thickening agents, stabilizers, disintegrating agents, defoaming agents, warning colors and film-forming agents.

8. The insecticidal composition according to claim 1, wherein The dosage form is wettable powder, water dispersible granules, granules, suspension, suspoemulsion, microemulsion, emulsion, dispersible oil suspension, seed treatment dispersible powder or seed treatment suspension.

9. The insecticidal composition according to any one of claims 1 to 8, characterized in that, The effective components isothiazolyl amide and triflumezopyr in the insecticidal composition synergistically promote insecticidal effect.

10. Use according to claim 9, characterized in that, The leaf-eating pests are leaf-eating pests on vegetable, cotton, fruit trees, corn, tobacco, flowers, melons or legume crops.

Images