Microbial agent suitable for animal feed

Abstract

The invention relates to the technical field of microbial preparations, and provides a microbial agent suitable for animal feed, which not only can improve the litter size and live litter size, but also can improve the birth weight of piglets and improve the survival rate of the piglets on the basis of solving constipation of sows by adopting probiotics. A microbial agent suitable for animal feed comprises 15 parts of bacterial powder, 45 parts of corn starch, 12 parts of skim milk powder, 8 parts of a yeast extract, 7 parts of a soybean extract and 6 parts of a rice bran extract. The bacterial powder is pediococcus pentosaceus bacterial powder, and the viable count of pediococcus pentosaceus in the microbial agent is not less than 1 * 10 < 6 > CFU / g.

Description

Technical Field

[0001] This invention relates to the field of microbial preparation technology, and more specifically to a microbial agent suitable for animal feed. Background Technology

[0002] Constipation is common in sows in intensive poultry farms, typically affecting 20%-35% of cases, and sometimes reaching as high as 50%. It mainly occurs in late pregnancy and during the pre- and postpartum periods. Constipation in sows should not be ignored. Accumulation of feces in the intestines leads to the absorption of toxins, potentially damaging liver and kidney function and further causing a decline in cardiopulmonary function. This is particularly serious during farrowing, as decreased cardiopulmonary function can reduce oxygen supply to the uterus, increasing the risk of dystocia and stillbirth.

[0003] Existing technologies include adding magnesium sulfate to feed during gestation to reduce sow constipation rates and increase sows' lying-down time. However, this can also inhibit uterine contractions, prolong labor, lead to weak delivery, and affect farrowing success and survival rates. Other methods involve adding lactic acid bacteria and Bacillus to feed to regulate the sow's intestinal flora balance, alleviating constipation during gestation (reducing constipation incidence by 50%) and decreased feed intake. Simultaneously, by lowering the levels of inflammatory factors, it reduces the risk of abortion and stillbirth, overcoming the aforementioned technical problems caused by magnesium sulfate. Based on this, we propose a microbial agent suitable for animal feed. In addition to using probiotics to address sow constipation, it not only increases litter size and live birth rate but also increases piglet birth weight and survival rate. Summary of the Invention

[0004] This invention provides a microbial inoculant suitable for animal feed, effectively solving the above-mentioned technical problems.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A microbial agent suitable for animal feed, characterized in that its raw materials, by weight, include 15 parts of bacterial powder, 45 parts of corn starch, 12 parts of skim milk powder, 8 parts of yeast extract, 7 parts of soybean extract, and 6 parts of rice bran extract. The bacterial powder includes *Pediococcus pentosaceus* powder, and the viable count of *Pediococcus pentosaceus* in the microbial agent is not less than 1 × 10⁻⁶. 6 CFU / gram.

[0006] Furthermore, the raw materials include 5 parts of compound amino acids.

[0007] Furthermore, the raw material includes 2 parts of silicon dioxide.

[0008] Furthermore, the bacterial powder is composed of bacterial powders from three bacteria: Pediococcus pentosaceus, Bacillus subtilis, and Bacillus vesalis. The ratio of viable bacteria of Pediococcus pentosaceus, Bacillus subtilis, and Bacillus vesalis is 1:1:1, and the total number of viable bacteria of Pediococcus pentosaceus, Bacillus subtilis, and Bacillus vesalis in the microbial agent is not less than 1 × 10⁻⁶. 6 CFU / gram.

[0009] Furthermore, the above-mentioned microbial inoculant is prepared by the following method: S1. Weigh the raw materials of each component; S2. Premix the weighed bacterial powder with an equal weight of corn starch in a mixer for 5-10 minutes; S3. Add twice the weight of cornstarch to the initially mixed materials and continue mixing for 10-15 minutes. S4. Add the remaining ingredients to the mixer and mix for 45-60 minutes.

[0010] Furthermore, the bacterial powder is prepared by the following method: S11. Inoculate the bacterial cells into the fermenter, and ferment to obtain a viable cell concentration of not less than 5 × 10⁻⁶. 9 Fermentation broth with CFU / ml; S12. Centrifuge the fermentation liquid to obtain bacterial sludge; S13. Freeze-dry the mushroom mud to obtain mushroom powder.

[0011] Furthermore, in step S12, the centrifugation parameters are as follows: 4-10℃, 10000 ~ 15000 rpm, 10-20 min.

[0012] Furthermore, in step S13, the bacterial sludge obtained in step S11 is mixed with a freeze-drying protectant at a volume ratio of 1:1. The freeze-drying protectant comprises, by mass fraction, 10% skim milk powder, 3% sodium L-ascorbate, 2% trehalose, and the remainder is water. The pre-freezing temperature is -50~-40℃, the freezing temperature is -25~-20℃, the vacuum degree is 0.1 mbar, and the final freeze-drying temperature is 25~30℃.

[0013] Furthermore, the animal in question is a domestic animal or poultry.

[0014] Furthermore, the preferred type of pig among the livestock is a first-parity sow.

[0015] This invention provides a microbial inoculant suitable for animal feed, which has the following beneficial effects: 1. The core ingredient, Pediococcus pentosaceus, can promote the targeted proliferation of beneficial microorganisms in the sow's intestines, inhibit the growth of harmful bacteria, optimize the intestinal microecological environment, and enhance digestive and absorptive functions, thereby improving overall intestinal health; 2. The formula contains abundant bacterial powder and dietary fiber sources (such as corn starch, rice bran and soybean extract), which work synergistically to promote intestinal peristalsis, increase fecal moisture, reduce constipation problems common in pregnant sows, and improve the comfort and health of sows. 3. By improving nutrient absorption and metabolism, it ensures the sow's physical strength and nutritional reserves during pregnancy, supports embryo implantation and development, and thus effectively increases the number of piglets per litter, the number of live piglets, and the birth weight of piglets.

[0016] 4. The microbial agent of the present invention also shows good performance when applied to conventional livestock such as piglets, laying hens, broilers, and beef cattle, which can effectively improve the survival rate of the farmed animals, and at the same time reduce the odor of the farm and optimize the environment. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Example 1

[0019] A microbial agent suitable for animal feed, the raw materials of which, by weight, include 15 parts of bacterial powder, 45 parts of corn starch, 12 parts of skim milk powder, 8 parts of yeast extract, 7 parts of soybean extract, 6 parts of rice bran extract, 5 parts of compound amino acids, and 2 parts of silicon dioxide. The bacterial powder is composed of bacterial powders from three bacteria: Pediococcus pentosaceus, Bacillus subtilis, and Bacillus belyssus. The ratio of viable bacteria of Pediococcus pentosaceus, Bacillus subtilis, and Bacillus belyssus is 1:1:1, and the total number of viable bacteria of Pediococcus pentosaceus, Bacillus subtilis, and Bacillus belyssus in the microbial agent is not less than 1 × 10⁻⁶. 6 CFU / gram.

[0020] The above-mentioned microbial inoculant was prepared by the following method: S1. Weigh the raw materials of each component; S2. Premix the weighed bacterial powder with an equal weight of corn starch in a mixer for 5-10 minutes; S3. Add twice the weight of cornstarch to the initially mixed materials and continue mixing for 10-15 minutes. S4. Add the remaining ingredients to the mixer and mix for 45-60 minutes.

[0021] The mycelial powders of three bacteria—Pediococcus pentosaceus, Bacillus subtilis, and Bacillus belyssus—were all prepared by the following methods: S11. Inoculate the bacterial cells into the fermenter, and ferment to obtain a viable cell concentration of not less than 5 × 10⁻⁶. 9 Fermentation broth with CFU / ml; S12. Centrifuge the fermentation liquid to obtain bacterial sludge. The centrifugation parameters are as follows: 4-10℃, 10000 ~ 15000 rpm, 10-20min. S13. Mix the bacterial sludge obtained in step S11 with the freeze-drying protectant at a volume ratio of 1:1. The freeze-drying protectant, by mass fraction, includes 10% skim milk powder, 3% sodium L-ascorbate, 2% trehalose, and the remainder is water. The pre-freezing temperature is -50~-40℃, the freezing temperature is -25~-20℃, the vacuum degree is 0.1 mbar, and the final freeze-drying temperature is 25~30℃ to obtain the bacterial powder.

[0022] Then, the bacterial powders of three bacteria—Pediococcus pentosaceus, Bacillus subtilis, and Bacillus belye—were mixed together.

[0023] Comparison Example

[0024] Application Example 1 In this application example, the microbial agents from Example 1 and Control Examples 1-4 were added to the feed of first-parity sows (Landrace pigs) at a rate of 15 grams per sow per day. The agents were also added to the feed of sows preparing for pregnancy and breeding boars. The number of piglets born, the number of live piglets, and the birth weight of piglets were observed in each group of 20 first-parity sows. Control group 5 involved replacing *Pediococcus pentosaceus* in the microbial agent with *Lactobacillus acidophilus* of the same viable count.

[0025]

[0026] The effects of feed containing added microbial agents on the reproductive performance of first-parity sows were investigated. Compared with group B, groups A and CF showed significant improvements in litter size, live piglet count, and piglet birth weight. Group A had 9 more piglets and 11 more live piglets than group B, and the average birth weight of piglets in the experimental group was 0.19 kg / head higher than that in the control group. Among the A and CF groups using the same microbial strain but different formulations, group A showed better performance.

[0027] Application Example 2 In this application example, the microbial agent of Example 1 was added to the feed of laying hens. 800 healthy Hy-Line laying hens were divided into 4 groups of 200 each. The two treatment groups received 500 grams of the microbial agent of Example 1 per ton of feed, while the control group received an equal amount of the microbial agent of Control Group 5 (see Application Example 1 above).

[0028] The egg production rate of the group using this product remained at 96%, with an increase of 8.43%. At the same time, the live microorganisms have a special effect on maintaining the balance of intestinal flora, which can reduce the occurrence of diseases, reduce the incidence of disease in laying hens, and improve the laying cycle.

[0029] Application Example 3 In this application example, the microbial agent from Example 1 above is added to the feed of beef cattle. Twenty Simmental crossbred beef cattle in the fattening period were divided into two groups and raised separately in separate sheds for 60 days. The group receiving the feed received a basic diet supplemented with the microbial agent from Example 1 at each meal, while the control group was fed normally without the addition of microbial agent.

[0030] After using the powder for about 20 days, the cattle in the group that used it had a brighter coat, increased vitality, reduced fecal output, decreased odor in the farm, higher average daily weight gain, and improved overall efficiency by 16.98%.

[0031] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0032] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A microbial inoculant suitable for animal feed, characterized in that: Its raw materials, by weight, include 15 parts of bacterial powder, 45 parts of corn starch, 12 parts of skim milk powder, 8 parts of yeast extract, 7 parts of soybean extract, and 6 parts of rice bran extract. The bacterial powder includes *Pediococcus pentosaceus* powder, and the viable count of *Pediococcus pentosaceus* in the microbial agent is not less than 1 × 10⁻⁶. 6 CFU / gram.

2. The microbial inoculant for animal feed as described in claim 1, characterized in that: The raw materials include 5 parts of compound amino acids.

3. The microbial agent for animal feed as described in claim 2, characterized in that: The raw materials include 2 parts of silicon dioxide.

4. The microbial agent for animal feed as described in claim 3, characterized in that: The bacterial powder is composed of bacterial powders from three bacteria: *Pediococcus pentosaceus*, *Bacillus subtilis*, and *Bacillus belyssae*. The ratio of viable counts of *Pediococcus pentosaceus*, *Bacillus subtilis*, and *Bacillus belyssae* is 1:1:1, and the total viable count of *Pediococcus pentosaceus*, *Bacillus subtilis*, and *Bacillus belyssae* in the microbial agent is not less than 1 × 10⁻⁶. 6 CFU / gram.

5. A microbial inoculant for animal feed as described in any one of claims 1-4, characterized in that: The above-mentioned microbial inoculant was prepared by the following method: S1. Weigh the raw materials of each component; S2. Premix the weighed bacterial powder with an equal weight of corn starch in a mixer for 5-10 minutes; S3. Add twice the weight of cornstarch to the initially mixed materials and continue mixing for 10-15 minutes. S4. Add the remaining ingredients to the mixer and mix for 45-60 minutes.

6. A microbial agent suitable for animal feed as described in claim 5, characterized in that: The bacterial powder is prepared by the following method: S11. Inoculate the bacterial cells into the fermenter, and ferment to obtain a viable cell concentration of not less than 5 × 10⁻⁶. 9 Fermentation broth with CFU / ml; S12. Centrifuge the fermentation liquid to obtain bacterial sludge; S13. Freeze-dry the mushroom mud to obtain mushroom powder.

7. A microbial inoculant for animal feed as described in claim 6, characterized in that: In step S12, the centrifugation parameters are as follows: 4-10℃, 10000 ~ 15000 rpm, 10-20 min.

8. A microbial inoculant for animal feed as described in claim 6, characterized in that: In step S13, the bacterial sludge obtained in step S11 is mixed with the freeze-drying protectant at a volume ratio of 1:

1. The freeze-drying protectant, by mass fraction, comprises 10% skim milk powder, 3% sodium L-ascorbate, 2% trehalose, and the remainder is water. The pre-freezing temperature is -50~-40℃, the freezing temperature is -25~-20℃, the vacuum degree is 0.1 mbar, and the final freeze-drying temperature is 25~30℃.

9. A microbial inoculant for animal feed as described in any one of claims 1-4, characterized in that: The animals mentioned are livestock or poultry.

10. A microbial inoculant for animal feed as described in claim 9, characterized in that: The pigs mentioned are first-parity sows.

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