Streptomyces mannivofaciens, culture, composition, microbial agent and application of streptomyces mannivofaciens

Abstract

The invention belongs to the technical field of microbiology. The embodiment discloses a Streptomyces manniprovofaciens strain, a culture, a composition, a microbial agent and application thereof. The bacterial strain is a bacterial strain preserved in Guangdong Microbial Culture Collection Center with a preservation number of GDMCC No.64195, or a bacterial strain with a 16S rRNA gene sequence having at least 98.65% identity with a sequence represented by SEQ ID No: 1, or a bacterial strain with a genomic sequence having at least 95% average nucleotide consistency with the bacterial strain with the preservation number of GDMCC 64195, or a bacterial strain with a comparison fraction of at least 60%. The bacterial strain MN327951 provided by the embodiment of the invention shows relatively strong antibacterial activity and has a good siderophore producing characteristic. The strain MN327951 fermentation broth can significantly weaken the pathogenicity of ralstonia solanacearum to tomato seedlings, and the supernatant of the strain MN327951 fermentation broth has strong toxicity to Frankliniella occidentalis.

Description

Technical Field

[0001] This application relates to the field of microbiology, and in particular to Streptomyces manipuli, cultures, compositions, microbial agents and their applications. Background Technology

[0002] Streptomyces sp. belongs to the kingdom Prokaryotes, order Actinobacterales, and family Streptomycetaceae.

[0003] Streptomyces have multiple functions. Some can produce antibiotics and plant growth hormones, promoting plant growth and enhancing their resistance to biotic and abiotic stresses. Streptomyces primarily promote plant growth by secreting plant growth regulators such as IAAs and ferrophosphates. They can also assist some microorganisms in nitrogen fixation and improve the absorption of elements such as phosphorus and potassium. The metabolites secreted by Streptomyces contain various types of antibacterial compounds and can help plants resist infection by pests and diseases.

[0004] Researching Streptomyces with favorable characteristics and promoting their widespread application in agricultural production is of great significance. Summary of the Invention

[0005] This application aims to at least solve one of the technical problems existing in the prior art. To this end, embodiments of the present invention provide a *Streptomyces manipur* strain selected from any one of A1) to A3): A1) a strain deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No. 64195; A2) a 16S rRNA gene sequence and SEQ ID... The sequence shown in No. 1 has at least 98.65%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, or 100% identity with the sequence shown in No. 1; strains whose genome sequence has at least 95%, 96%, 97%, 98%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, or 100% average nucleotide identity with A1); and / or strains whose genome sequence has an alignment score ≥60%, ≥65%, ≥70%, ≥78%, ≥80%, ≥85%, ≥90%, ≥95%, ≥96%, ≥97%, ≥98%, or ≥99% with the genome of A1).

[0006] According to another aspect of the invention, a culture of *Streptomyces manipulpur* strain is provided, comprising any one of B1) to B5): B1) fermentation medium of the *Streptomyces manipulpur* strain; B2) supernatant of fermentation medium of the *Streptomyces manipulpur* strain; B3) concentrated product of B1) or B2); B4) extract of B1) or B2); and B5) dried product of any one of B1) to B4).

[0007] Preferably, the fermentation medium is a liquid culture medium;

[0008] Preferably, the culture temperature is 28–30°C, and / or the culture time is 7–14 days.

[0009] According to another aspect of the invention, a composition is provided comprising at least one of the aforementioned Streptomyces mannipur strains and / or their metabolites, cultures, fermentation broths, or extracts.

[0010] Preferably, the composition further comprises additional surfactants and / or excipients;

[0011] Preferably, the adjuvant is selected from one or more other biological control agents, one or more other microbial control agents, one or more chemical agents, one or more fertilizers, one or more herbicides, one or more growth promoters, or any combination thereof;

[0012] Preferably, the excipients are selected from agriculturally or horticulturally acceptable carriers, diluents, stabilizers, fillers, wetting agents, colorants, solvents, co-solvents, film-forming agents, spontaneous growth promoters, emulsifiers, dispersants, preservatives, antifreeze agents, thickeners, adjuvants, or any combination thereof;

[0013] Preferably, the one or more additional biocontrol agents are selected from bacteria, fungi, viruses, actinomycete biocontrol agents, insect biocontrol agents, oviposition and worm biocontrol agents, nematode biocontrol agents, or any combination thereof;

[0014] Preferably, the one or more additional microbial control agents are selected from strains of Trichoderma, Bacillus, or any combination thereof, or from metabolites of Trichoderma, Bacillus, or any combination thereof, or from fermentation broth or fermentation supernatant of Trichoderma, Bacillus, or any combination thereof.

[0015] Preferably, the bacteria of the genus Bacillus are selected from: Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans, Paenibacillus polymyxa, Bacillus amyloliquefaciens, or any combination thereof;

[0016] Preferably, the one or more additional biocontrol agents are selected from at least one of Trichoderma harzianum, Purpureocillium lilacinum, Penicillium bilaiae, Bacillus subtilis, Burkholderiarinojensis, Bacillus pumilus, Bacillus velezensis, Methylobacterium rhodesianum, Methylobacterium extorquens, and Paenibacillus peoriae;

[0017] Preferably, the *Trichoderma harzianum* is *Trichoderma harzianum* with accession number CGMCC No. 15679;

[0018] Preferably, the *Paecilomyces lilacinus* is *Paecilomyces lilacinus* with accession number CGMCC No. 12773;

[0019] Preferably, the Penicillium baqensis is Penicillium baqensis with accession number CGMCC No. 12767;

[0020] Preferably, the Bacillus subtilis is Bacillus subtilis with accession number CGMCC No. 12908;

[0021] Preferably, the *Rhodotorula buergerianum* is *Rhodotorula buergerianum* with accession number GDMCC No. 61156;

[0022] Preferably, the *Bacillus pumilus* is the *Bacillus pumilus* with accession number GDMCC No. 61962;

[0023] Preferably, the Bacillus belyssus is Bacillus belyssus with accession number GDMCC No. 61434;

[0024] Preferably, the *Methylobacterium roximatee* is *Methylobacterium roximatee* with accession number GDMCC No. 60729;

[0025] Preferably, the *Methylobacterium tumefaciens* is *Methylobacterium tumefaciens* with accession number GDMCC No. 62943;

[0026] Preferably, the *Bacillus pilaris* is *Bacillus pilaris* with accession number GDMCC No. 60482;

[0027] Preferably, the composition is in solid form, liquid form, powder form, or any combination thereof;

[0028] Preferably, the composition is selected from at least one of solutions, powders, granules, emulsions, emulsions, suspensions, tablets, microparticles, and wettable powders.

[0029] According to another aspect of the present invention, a microbial inoculant is provided, comprising at least one of the aforementioned Streptomyces mannipur strains and / or their metabolites, cultures, or compositions.

[0030] Preferably, the microbial agent further comprises agriculturally or horticulturally acceptable diluents, fillers, solvents, accelerators, carriers, emulsifiers, dispersants, preservatives, antifreeze agents, thickeners, adjuvants, or any combination thereof;

[0031] Preferably, the microbial agent is a powder, granules, or liquid.

[0032] According to another aspect of the invention, a microbial insecticide, microbial fertilizer, microbial pesticide, or biostimulant is provided, comprising at least one of the aforementioned Streptomyces manipula strains and / or their metabolites, cultures, or compositions.

[0033] According to another aspect of the present invention, a plant growth regulator, seed coating agent, root drenching agent, seed soaking agent, microbial pesticide or microbial fertilizer is provided, comprising at least any one of the aforementioned Streptomyces manipula strains and / or their metabolites, cultures, or compositions.

[0034] According to another aspect of the present invention, the use of the aforementioned Streptomyces manipulpur strains, cultures, compositions, microbial agents, microbial insecticides or microbial fertilizers or microbial pesticides or biostimulants or plant growth regulators, seed coating agents, root drenching agents, seed soaking agents, microbial pesticides or microbial fertilizers in the preparation of antifungal products, antibacterial products, seed coating products, biofertilizers, root drenching agents, seed soaking agents, microbial pesticides or microbial fertilizers is provided.

[0035] According to another aspect of the present invention, the aforementioned Streptomyces manipula strains, cultures, compositions, microbial agents, microbial insecticides or microbial fertilizers or microbial pesticides or biostimulants or plant growth regulators, seed coating agents, root irrigation agents, seed soaking agents, microbial pesticides or microbial fertilizers are provided for the application in the prevention and control of plant diseases and pests.

[0036] Preferably, the plant diseases and pests include infectious and / or non-infectious diseases and pests;

[0037] Preferably, the plant diseases and pests include those caused by pathogenic bacteria, pathogenic fungi, nematodes, insect eggs and / or pests;

[0038] Preferably, the pathogenic fungi include at least one of the following: Colletotrichum, Rhizoctonia, Fusarium, Magnaporthe, and Phytophthora.

[0039] Preferably, the pathogenic fungi include at least one of the following: Colletotrichum siamense, Rhizoctonia solani, Fusarium graminearum, Magnaphortheoryzae, and Phytophthora sojae;

[0040] Preferably, the pathogenic bacteria include at least one of the following: Pectobacterium, Pseudomonas, Xanthomonas, and Ralstonia.

[0041] Preferably, the pathogenic bacteria include at least one of the following: *Pectobacterium carotovorum subsp. carotovorum*, *Pseudomonas syringae pv. tomato*, *Xanthomonas axonopodis pv. citri*, *Ralstonia solanacearum*, and *Xanthomonas oryzae pv. oryzae*.

[0042] Preferably, the pest is a thrips;

[0043] Preferably, the plant disease includes at least one of damping-off, Fusarium wilt, Fusarium head blight, root rot, stem base rot, anthracnose, rice blast, bacterial blight, and citrus canker;

[0044] Preferably, the plant is selected from food crops, cash crops, or root and tuber crops;

[0045] Preferably, the food crop is selected from at least one of cereal crops, tuber crops, and legume crops;

[0046] Preferably, the cereal crops include at least one of the following grasses: rice, wheat, corn, barley, oats, rye, sorghum, millet, foxtail millet, barnyard millet, and buckwheat; preferably, the tuber crops include at least one of the following: sweet potato, yam, and potato; preferably, the legume crops include at least one of the following: soybean, broad bean, pea, mung bean, and peanut.

[0047] Preferably, the economic crop includes at least one of the following: plants of the Solanaceae family, Rosaceae family, Rutaceae family, Musaceae family, Cucurbitaceae family, Brassicaceae family, Orchidaceae family, Fabaceae family, Asteraceae family, Liliaceae family, Zingiberaceae family, Passifloraceae family, Bromeliaceae family, Araliaceae family, and Cactaceae family;

[0048] Preferably, the Solanaceae plant includes at least one of the following: tomato, pepper, potato, eggplant; preferably, the Rosaceae plant includes at least one of the following: strawberry, papaya; preferably, the Rutaceae plant includes citrus; preferably, the Musaceae plant includes banana; preferably, the Cucurbitaceae plant includes at least one of the following: cucumber, winter melon, pumpkin, bitter melon, loofah, watermelon, monk fruit; preferably, the Cruciferae plant includes at least one of the following: Chinese cabbage, rapeseed, kale, radish, cauliflower; preferably, the Orchidaceae plant includes orchid; preferably, the Fabaceae plant includes soybean; preferably, the Asteraceae plant includes lettuce; preferably, the Liliaceae plant includes garlic; preferably, the Zingiberaceae plant includes ginger; preferably, the Passifloraceae plant includes passion fruit; preferably, the Bromeliaceae plant includes pineapple; preferably, the Araliaceae plant includes Panax notoginseng; preferably, the Cactaceae plant includes dragon fruit.

[0049] Preferably, the root and tuber crops include at least one of the following: potatoes, carrots, leafy vegetables, solanaceous vegetables, strawberries, grapes, citrus fruits, bananas, kiwifruit, dragon fruit, tomatoes, peppers, beans, ginger, Panax notoginseng, and ginseng.

[0050] According to another aspect of the present invention, the application of the aforementioned *Streptomyces manipur* strains, cultures, compositions, microbial inoculants, microbial insecticides or microbial fertilizers or microbial pesticides or biostimulants or plant growth regulators, seed coating agents, root drenching agents, seed soaking agents, microbial pesticides or microbial fertilizers in promoting plant growth is provided.

[0051] Preferably, promoting plant growth includes: promoting the absorption of nutrients by the plant.

[0052] Preferably, promoting plant growth includes promoting the absorption of iron ions by the plant.

[0053] According to another aspect of the present invention, a method for preventing and controlling plant diseases and pests, improving crop resistance to adversity and / or promoting plant growth is provided, comprising applying to plants or seeds the aforementioned Streptomyces manipulator strains, cultures, compositions, microbial agents, microbial insecticides or microbial fertilizers or microbial pesticides or biostimulants or plant growth regulators, seed coating agents, root irrigation agents, seed soaking agents, microbial pesticides or microbial fertilizers.

[0054] The strain MN327951 provided in the embodiments of the present invention exhibits strong antibacterial activity and good siderophore production characteristics. The fermentation broth of strain MN327951 can significantly weaken the pathogenicity of bacterial wilt to tomato seedlings, and the supernatant of the fermentation broth of strain MN327951 has strong virulence against western flower thrips.

[0055] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0056] Figure 1 The image shows a photograph of the colony morphology of strain MN327951;

[0057] Figure 2 Microscopic morphology photographs of strain MN327951 are shown;

[0058] Figure 3 The Gram staining results of strain MN327951 are shown;

[0059] Figure 4 A schematic diagram of the phylogenetic tree of strain MN327951 is shown;

[0060] Figure 5 A schematic diagram showing the antifungal effect of strain MN327951 against different plant pathogenic fungi is shown.

[0061] Figure 6 A schematic diagram showing the antibacterial effect of strain MN327951 against different plant pathogenic bacteria is shown.

[0062] Figure 7 A schematic diagram shows the formation of siderophore chelate rings by strain MN327951 on a CAS detection plate.

[0063] Preservation information:

[0064] Streptomyces manipurensis strain MN327951, deposited at the Guangdong Microbial Culture Collection Center (GDMCC) with accession number GDMCC No. 64195, on December 26, 2023, at the Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou. The proposed taxonomic name is Streptomyces manipurensis. Detailed Implementation

[0065] The following will clearly and completely describe the concept and technical effects of this application in conjunction with embodiments, so as to fully understand the purpose, features and effects of this application. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are all within the scope of protection of this application.

[0066] The embodiments of this application are described in detail below. The described embodiments are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0067] In the description of this application, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number, and "approximately" means within the range of ±20%, 10%, 8%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.2%, 0.1%, etc. of the stated number. The use of "first" and "second" is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0068] In the description of this application, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0069] The term "strain" refers to a member of a bacterial species that possesses genetic characteristics that distinguish it from closely related members of the same bacterial species. These genetic characteristics can include the complete or partial absence of at least one gene, the complete or partial absence of at least one regulatory region (e.g., promoter, terminator, riboswitch, ribosome binding site), the presence of at least one recombinant gene, the presence of at least one mutant gene, the presence of at least one exogenous gene (a gene from another species), the presence of at least one non-natural plasmid, the presence of at least one antibiotic resistance cassette, or a combination thereof. A pure culture of any microorganism isolated from nature can be called a strain of microorganism, and a variant of a strain obtained experimentally (e.g., through mutagenesis) can also be called a new strain to distinguish it from the original strain. The strain is the most fundamental operational entity in microbial classification and research. Genetic characteristics between different strains can be identified by PCR amplification, optionally followed by DNA sequencing of the genomic region of interest or the entire genome. In cases where a strain (compared to another strain of the same species) acquires or loses antibiotic resistance or acquires or loses biosynthetic capacity (e.g., auxotrophic strains), the strain or nutrient / metabolite can be distinguished by selection or anti-selection using antibiotics.

[0070] It is known in this art that bacterial species can be classified and identified using both traditional classification methods and molecular biological methods. Traditional classification methods include, for example, cell morphology observation, Gram staining, flagellar staining, and various metabolic experiments. Molecular biological methods include ribosomal RNA sequencing and whole-genome sequencing-based methods.

[0071] 16S rRNA is a type of ribosomal RNA found in prokaryotes. The 16S rRNA gene sequence consists of variable and conserved regions. The conserved regions are common to all bacteria, while the variable regions vary to varying degrees among different bacteria. By comparing the 16S rRNA gene sequences of bacteria and analyzing their evolutionary distances based on sequence differences, an evolutionary tree can be constructed.

[0072] When the 16S rRNA gene sequence identity between two strains is less than 98.65%, they can be identified as belonging to different species (see Kim, M., Oh, H.-S., Park, S.-C., & Chun, J. (2014). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequences similarity for species demarcation of prokaryotes. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(Pt 2), 346-351, and Liu, C., Du, M.-X., Abuduaini, R., Yu, H.-Y., Li, D.-H., Wang, Y.-J., Liu, S.-J. (2021). Enlightening the taxonomic darkness of human gut microbiomes with a culturedbiobank. Microbiome, 9(1), page P23; Pablo Yarza, Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences, Microbiology, volume 12, pages P635-P645, 2014).

[0073] When the identity between the 16S rRNA gene sequences of two strains is less than 95%, they can be determined to belong to different genera (“Agenus definition for Bacteria and Archaea based on genome relatedness and taxonomic affiliation”, RABarco, bioRxiv (doi: https: / / doi.org / 10.1101 / 392480); Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis, Jethro S. Johnson, Nature Communications 10, Article number: 5029 (2019)).

[0074] The first aspect of this application relates to Streptomyces manipurensis strain MN327951, deposited at the Guangdong Microbial Culture Collection Center (GDMCC) with accession number GDMCC No. 64195, deposited on December 26, 2023, at the Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou. The proposed taxonomic name is Streptomyces manipurensis.

[0075] The 16S rRNA gene sequence of strain MN327951 is shown in SEQ ID No. 1.

[0076] In some embodiments of this application, variants of strain MN327951 based on the aforementioned 16S rRNA gene sequence are provided. These strain variants exhibit at least 98.65%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, and 100% identity in their 16S rRNA gene sequences compared to the aforementioned strain, and are therefore strains belonging to the same species or genus but different species. It is reasonable to infer that these strains of the same species or genus but different species have similar biological activities. In other embodiments, the variants exhibit at least 98.65%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, and 100% identity in their 16S rRNA gene sequences compared to the sequence shown in SEQ ID No. 1.

[0077] The “identity” between the sequences of two nucleic acid molecules can be determined using known computer algorithms, such as at least one of the GCG package, BLASTN, or FASTA. Other commercially or publicly available programs may include, for example, the DNAStar “MegAlign” program.

[0078] Furthermore, with the rapid development of second- and third-generation sequencing technologies, bacterial species identification based on whole-genome sequencing has become possible, making the identification results more accurate. Average nucleotide identity (ANI) refers to the similarity of homologous genes between two bacterial genomes; alignment fraction (AF) refers to the percentage of orthologous regions shared between two genomes. In the field of bacterial taxonomy, it is generally believed that an ANI value of 95% or higher is required to identify bacteria as belonging to the same species (Kim, M., Oh, H.-S., Park, S.-C., & Chun, J. (2014). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(Pt 2), 346–351, and Liu, C., Du, M.-X., Abuduaini, R., Yu, H.-Y., Li, D.-H., Wang, Y.-J., Liu, S.-J. (2021). Enlightening the taxonomy darkness of human gut microbiomes with a cultured biobank. Microbiome, 9(1), p. 23; Pablo Yarza, Uniting the classification of cultured and uncultured bacteria and archaea using16SrRNA gene sequences, Microbiology, volume 12, pages P635-P645, 2014).

[0079] When the identity between the 16S rRNA gene sequences of two strains is less than 95%, they can be determined to belong to different genera (“Agenus definition for Bacteria and Archaea based on genome relatedness and taxonomic affiliation”, RABarco, bioRxiv (doi: https: / / doi.org / 10.1101 / 392480); Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis, Jethro S. Johnson, Nature Communications 10, Article number: 5029 (2019)).

[0080] Using the methods described above, those skilled in the art can determine whether an isolated strain belongs to a different species or is a new strain of the same species as strain MN327951. For example, when the average nucleotide similarity (ANI) value of strain MN327951 is at least 95%, such as at least 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, or 100%, it can be determined that the two belong to the same genus or the same species.

[0081] Currently, there are various mature tools available for calculating ANI values, such as the local calculation software Jspecies ( / jspecies) and Gegenees ( / documentation.html), and the online calculation tools ANI caculator (http: / / enveomics.gatech.edu / ), EzGenome ( / ezgenome / ani), and ANItools. Therefore, the ANI values ​​of the isolated strain and strain MN327951 can be calculated using any of the aforementioned local calculation software, online calculation tools, or other tools or methods known in the art.

[0082] Therefore, in some embodiments of this application, variants of strain MN327951 are provided. These variants have at least 95%, 96%, 97%, 98%, 98.5%, 98.65%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, and 100% average nucleotide identity with strain MN327951 in terms of genome, and are thus strains of the same species or different species within the same genus as strain MN327951. It is reasonable to infer that these strains of the same species or different species within the same genus have similar biological activities. Furthermore, this can also be determined by the alignment score of the corresponding genomes. For example, variants with an alignment score of at least 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, and 99% compared to strain MN327951 also have the same or similar biological activities.

[0083] In some embodiments, based on the alignment results of the 16S rRNA gene sequence and ANI, the strain variant has at least 98.65%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, and 100% identity with the 16S rRNA gene sequence of strain MN327951, and at the same time, has at least 95%, 96%, 97%, 98%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, and 100% average nucleotide identity with the genome of strain MN327951.

[0084] The term "variant" includes any bacteria derived from strain MN327951, such as bacteria resulting from mutations or recombinations occurring through natural mutations or recombinations (e.g., mutations caused by cell proliferation or cell division), resistance selection, or any other mechanism such as radiation, viruses, transposons, or mutagenic chemicals. Furthermore, variants also include other strains isolated from the natural environment that have the same or similar taxonomic characteristics and properties as strain MN327951, including strains of the same genus but different species, or other strains within the same species with different phenotypic variations.

[0085] The second aspect of the embodiments of this application relates to cultures of Streptomyces manipuli strains.

[0086] The term "culture" refers to a group of microbial cells or their growth within a specific time and space, such as slant cultures or shake flask cultures of microorganisms. Specifically, in this invention, it refers to the product obtained by culturing isolated bacterial strains in a fermentation medium. The fermentation medium can be natural or artificial, for example, a solid or liquid medium. In some embodiments, the fermentation medium includes at least one of peptones, such as meat peptone, casein peptone, whey peptone, or soybean peptone. Additionally, it may include other inorganic salts or organic substances used to promote bacterial growth.

[0087] The Streptomyces disclosed herein are suitable for cultivation or fermentation on common actinomycete culture media, including but not limited to commercially available Gao's No. 1 medium, ISP2 medium, ISP4 medium, R2A medium, medium No. 1, medium No. 2, YM medium, EMB (malt broth) medium, PDB (potato dextrose broth) medium, CDB (Czapek Dox broth) medium, and SDB (Sabouraud dextrose broth) medium. The components of ISP4 medium include: 10.0g soluble starch, 1.0g dipotassium hydrogen phosphate, 1g magnesium sulfate, 1g sodium chloride, 2g ammonium sulfate, 2g calcium sulfate, 0.001g ferrous sulfate, 0.001g manganese chloride, 0.001g zinc sulfate, 15g agar, and water to a final volume of 1L, pH = 7.2 ± 0.2. The components of R2A medium include: 0.25g tryptone, 0.5g acid-hydrolyzed casein, 0.5g yeast extract, 0.5g soluble starch, 0.3g dipotassium hydrogen phosphate, 0.1g magnesium sulfate, 0.3g sodium pyruvate, 0.25g peptone, 0.5g glucose, and water to a final volume of 1L, with a pH of 7.2±0.2. Medium No. 1 includes soluble starch, soybean flour, MgSO4·7H2O, NaCl, K2HPO4, and FeSO4·7H2O; preferably, medium No. 1 includes 5–30g / L soluble starch, 5–15g / L soybean flour, 0.1–0.8g / L MgSO4·7H2O, 0.1–0.8g / L NaCl, 0.1–0.8g / L K2HPO4, and 0.01–0.05g / L... FeSO4·7H2O; a more preferred culture medium No. 1 is: 20 g / L soluble starch; 10 g / L soybean flour; 0.5 g / L MgSO4·7H2O; 0.5 g / L NaCl; 0.5 g / L K2HPO4; 0.01 g / L FeSO4·7H2O.

[0088] Culture medium No. 2 comprises: sucrose; skim milk powder; soybean meal; K2HPO4; FeSO4·7H2O; CaCO3; preferably, culture medium No. 2 consists of: 50–80 g / L sucrose; 0.5–2 g / L skim milk powder; 10–30 g / L soybean meal; 0.5–2 g / L K2HPO4; 0.05–0.2 g / L FeSO4·7H2O; 1–3 g / L CaCO3; more preferably, culture medium No. 2 consists of: 80 g / L sucrose; 1 g / L skim milk powder; 20 g / L soybean meal; 1 g / L K2HPO4; 0.1 g / L FeSO4·7H2O; 3 g / L CaCO3. YM medium, EMB (malt extract broth) medium, PDB (potato glucose broth) medium, CDB (Czapek Dox broth) medium, and SDB (Sabouraud glucose broth) medium are all well-known media in the field.

[0089] In a preferred embodiment, the activation medium for Streptomyces includes Gao's No. 1 medium or ISP2 medium. Gao's No. 1 medium, as described herein, comprises soluble starch, potassium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, sodium chloride, ferrous sulfate, agar, and water. In a more specific embodiment, Gao's No. 1 medium comprises 20g soluble starch, 0.5g sodium chloride, 0.01g ferrous sulfate, 1g potassium nitrate, 0.5g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 20g agar, and water to a final volume of 1L. ISP2 medium comprises: 4.0g yeast extract, 10.0g malt extract, 4.0g glucose, 20.0g agar, water to a final volume of 1L, and a pH of 7.3.

[0090] In a preferred embodiment, the liquid culture medium used for fermentation culture is medium No. 1 or medium No. 2.

[0091] In some embodiments, the culture can be a fermentation medium after bacterial culture, or the supernatant obtained by centrifugation or other methods from the fermentation medium, or a product obtained by concentrating the fermentation medium or its supernatant through at least one of the following methods: evaporation, freeze-drying, dialysis, extraction, membrane separation, etc., or an extract obtained by at least one of the following methods from the fermentation medium or its supernatant: extraction, solvent extraction (e.g., water or organic solvent), or a dried product obtained by drying any one of the fermentation medium or its supernatant, concentrated product, or extract. Furthermore, in some specific embodiments, the extract can be an extraction targeting one or more specific components, such as components with a specific molecular weight range, specific solubility, specific isoelectric point, etc.; or it can be an extraction not targeting one or more types of components. Specific extraction methods include extraction using at least one solvent such as methanol, ethanol, chloroform, water, acid, or alkali, following a specific process sequence and under certain temperature conditions. For example, extraction in 75% v / v ethanol at 60–80°C for 1–10 minutes, or in methanol at -30–-10°C for 1–10 minutes, or in a methanol / chloroform / aqueous solution at -40–-20°C for 30–60 minutes, or in deionized water at 95°C for 1–10 minutes, or in perchloric acid, trichloroacetic acid, hydrochloric acid, or sodium hydroxide solution at 0–4°C, etc., thereby extracting the corresponding metabolites, such as short-chain fatty acids or short-chain fatty acid salts, such as at least one of acetic acid, butyric acid, valeric acid, acetate, propionate, and valerate. Ion exchange, precipitation, and adsorption methods can also be used to obtain fermentation broth extracts.

[0092] It is understood that the aforementioned cultures contain compounds and / or cell debris from the strain, and / or metabolites secreted by the strain. These metabolites include small molecules (such as amino acids, nucleosides, and nucleotides) and large molecules (such as polypeptides, carbohydrates, nucleic acids, proteoglycans, and lipids). They can be primary metabolites directly involved in normal cellular functions, or secondary metabolites not typically required for basic cellular functions, as well as metabolic intermediates generated during the synthesis of primary or secondary metabolites. Therefore, different types of cultures containing the strain's compounds and / or metabolites can achieve biological functions similar to those of the strain. The metabolites can be purified and used alone or mixed with other substances.

[0093] A third aspect of the embodiments of this application relates to a composition comprising the aforementioned *Streptomyces manipula* strain and / or its metabolites, cultures, fermentation broths, or extracts. As used herein, "metabolite," "culture," "fermentation broth," and "extract" encompass any component, compound, substance, or byproduct (including, but not limited to, small molecule secondary metabolites) produced by the *Streptomyces manipula* strain described herein that has any of the beneficial effects described in this disclosure. The beneficial effects include heat resistance, low-temperature resistance, UV resistance, control of plant diseases and pests, and promotion of plant growth.

[0094] In some embodiments, the composition further comprises additional surfactants and / or excipients;

[0095] Preferably, the adjuvant is selected from one or more other biological control agents, one or more other microbial control agents, one or more chemical agents, one or more fertilizers, one or more herbicides, one or more growth promoters, or any combination thereof;

[0096] Preferably, the excipients are selected from agriculturally or horticulturally acceptable carriers, diluents, stabilizers, fillers, wetting agents, colorants, solvents, co-solvents, film-forming agents, spontaneous growth promoters, emulsifiers, dispersants, preservatives, antifreeze agents, thickeners, adjuvants, or any combination thereof;

[0097] Preferably, the one or more additional biocontrol agents are selected from bacteria, fungi, viruses, actinomycete biocontrol agents, insect biocontrol agents, oviposition and worm biocontrol agents, nematode biocontrol agents, or any combination thereof;

[0098] Preferably, the one or more additional microbial control agents are selected from strains of Trichoderma, Bacillus, or any combination thereof, or from metabolites of Trichoderma, Bacillus, or any combination thereof, or from fermentation broth or fermentation supernatant of Trichoderma, Bacillus, or any combination thereof.

[0099] Preferably, the bacteria of the genus Bacillus are selected from: Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans, Paenibacillus polymyxa, Bacillus amyloliquefaciens, or any combination thereof;

[0100] Preferably, the one or more additional biocontrol agents are selected from at least one of Trichoderma harzianum, Purpureocillium lilacinum, Penicillium bilaiae, Bacillus subtilis, Burkholderiarinojensis, Bacillus pumilus, Bacillus velezensis, Methylobacterium rhodesianum, Methylobacterium extorquens, and Paenibacillus peoriae;

[0101] Preferably, the *Trichoderma harzianum* is *Trichoderma harzianum* with accession number CGMCC No. 15679;

[0102] Preferably, the *Paecilomyces lilacinus* is *Paecilomyces lilacinus* with accession number CGMCC No. 12773;

[0103] Preferably, the Penicillium baqensis is Penicillium baqensis with accession number CGMCC No. 12767;

[0104] Preferably, the Bacillus subtilis is Bacillus subtilis with accession number CGMCC No. 12908;

[0105] Preferably, the *Rhodotorula buergerianum* is *Rhodotorula buergerianum* with accession number GDMCC No. 61156;

[0106] Preferably, the *Bacillus pumilus* is the *Bacillus pumilus* with accession number GDMCC No. 61962;

[0107] Preferably, the Bacillus belyssus is Bacillus belyssus with accession number GDMCC No. 61434;

[0108] Preferably, the *Methylobacterium roximatee* is *Methylobacterium roximatee* with accession number GDMCC No. 60729;

[0109] Preferably, the *Methylobacterium tumefaciens* is *Methylobacterium tumefaciens* with accession number GDMCC No. 62943;

[0110] Preferably, the *Bacillus pilaris* is *Bacillus pilaris* with accession number GDMCC No. 60482;

[0111] Preferably, the composition is in solid form, liquid form, powder form, or any combination thereof;

[0112] Preferably, the composition is selected from at least one of solutions, powders, granules, emulsions, emulsions, suspensions, tablets, microparticles, and wettable powders.

[0113] The adjuvants described herein refer to other biological and / or chemical agents with specific activities that are different from the strains contained in the composition. The adjuvants described herein are adjuvants that are compatible with the metabolites, cultures, fermentation broths, or extracts of the aforementioned bacterial strains.

[0114] In some embodiments, the aforementioned adjuvants may be phytonutrients, plant growth promoters (or growth stimulants), other biostimulants (micronutrients), fertilizers, herbicides, seed coating agents, etc.

[0115] Preferably, the fertilizer includes, but is not limited to, at least one of amino acid fertilizer, humic acid fertilizer, seaweed fertilizer, humic acid fertilizer, fertilizer containing pollen polysaccharides, polypeptide fertilizer, and microbial fertilizer.

[0116] The term "microbial fertilizer" refers to products containing specific microorganisms that are applied in agricultural production and can achieve specific fertilizer effects. These effects include not only the supply of nutrients to the soil, environment, and plants, but also the beneficial effects of the metabolites produced by the microorganisms on plants.

[0117] The terms "biofertilizer" and "plant growth promoter" are used interchangeably, referring to preparations that use organisms or their derivatives as the main active ingredient and have the effect of promoting plant growth. The terms "biopesticide" and "bioinsecticide" used in this specification are used interchangeably, referring to preparations that utilize organisms or their derivatives to kill or inhibit agricultural pests such as plant pathogens that cause plant diseases and pests.

[0118] In some embodiments, the aforementioned adjuvant active agent may include one or more chemical agents. In some embodiments, the chemical agent includes, but is not limited to, insecticides, bactericides, fungicides, viricides, nematicides, insecticides, acaricides, gastropod toxicants, herbicides, etc., or combinations thereof. In preferred embodiments, the aforementioned chemical agent includes one or more of bactericides, fungicides, and nematicides. Preferably, the aforementioned bactericides include, but are not limited to, one or more of kasugamycin, thiabendazole, copper oxychloride, thiabendazole, allicin, copper hydroxide, kasugamycin, cymoxanil, copper oxychloride, copper oxychloride, chlorobromoisocyanuric acid, trichloroisocyanuric acid, copper acetate, or copper succinate. Preferably, the aforementioned fungicides include, but are not limited to, one or more of carbendazim, mancozeb, aluminum fosetyl-aluminum, metalaxyl, metalaxyl, cymoxanil, dimethomorph, flumorph, silver oxychloride, benzalkonium chloride, cymoxanil, tebuconazole, or propiconazole. Preferably, the aforementioned nematicides include, but are not limited to, one or more of halogenated hydrocarbons, methyl thioisothiocyanate, organophosphates, carbamates, or avermectin.

[0119] According to a fourth aspect of the embodiments of this application, a microbial inoculant is provided, comprising at least any one of the aforementioned Streptomyces mannipur strains and / or their metabolites, cultures, or compositions.

[0120] Preferably, the microbial agent further comprises agriculturally or horticulturally acceptable diluents, fillers, solvents, accelerators, carriers, emulsifiers, dispersants, preservatives, antifreeze agents, thickeners, adjuvants, or any combination thereof;

[0121] Preferably, the microbial agent is a powder, granules, or liquid.

[0122] According to a fifth aspect of the embodiments of this application, a microbial insecticide, microbial fertilizer, microbial pesticide, or biostimulant is provided, comprising at least one of the aforementioned *Streptomyces manipurii* strains and / or their metabolites, cultures, or compositions. Biostimulants refer to substances that, when applied to soil and crops, can improve soil ecology, stimulate crop potential, and enhance crop systemic resistance, thereby increasing yield, quality, and agricultural production efficiency.

[0123] According to a sixth aspect of the embodiments of this application, a plant growth regulator, seed coating agent, root drenching agent, seed soaking agent, microbial pesticide or microbial fertilizer is provided, which includes at least any one of the aforementioned Streptomyces manipula strains and / or their metabolites, cultures, or compositions.

[0124] According to the seventh aspect of the embodiments of this application, the aforementioned Streptomyces manipur strain and / or its metabolites, cultures or compositions or biocontrol agents or biostimulants are used in the preparation of nematicide products, seed coating products, biofertilizers, root irrigation agents, seed soaking agents, microbial pesticides or microbial fertilizers.

[0125] According to the seventh aspect of the embodiments of this application, the aforementioned Streptomyces manipulator strains, cultures, compositions, microbial agents, microbial insecticides or microbial fertilizers or microbial pesticides or biostimulants or plant growth regulators, seed coating agents, root drenching agents, seed soaking agents, microbial pesticides or microbial fertilizers are used in the preparation of antifungal products, antibacterial products, seed coating products, biofertilizers, root drenching agents, seed soaking agents, microbial pesticides or microbial fertilizers.

[0126] According to the eighth aspect of the embodiments of this application, the aforementioned Streptomyces manipulpa strains, cultures, compositions, microbial agents, microbial insecticides or microbial fertilizers or microbial pesticides or biostimulants or plant growth regulators, seed coating agents, root irrigation agents, seed soaking agents, microbial pesticides or microbial fertilizers are provided for the application in the prevention and control of plant diseases and pests.

[0127] Preferably, the plant diseases and pests include infectious and / or non-infectious diseases and pests;

[0128] Preferably, the plant diseases and pests include those caused by pathogenic bacteria, pathogenic fungi, nematodes, insect eggs and / or pests;

[0129] Preferably, the pathogenic fungi include at least one of the following: Colletotrichum, Rhizoctonia, Fusarium, Magnaporthe, and Phytophthora.

[0130] Preferably, the pathogenic fungi include at least one of the following: Colletotrichum siamense, Rhizoctonia solani, Fusarium graminearum, Magnaphortheoryzae, and Phytophthora sojae;

[0131] Preferably, the pathogenic bacteria include at least one of the following: Pectobacterium, Pseudomonas, Xanthomonas, and Ralstonia.

[0132] Preferably, the pathogenic bacteria include at least one of the following: *Pectobacterium carotovorum subsp. carotovorum*, *Pseudomonas syringae pv. tomato*, *Xanthomonas axonopodis pv. citri*, *Ralstonia solanacearum*, and *Xanthomonas oryzae pv. oryzae*.

[0133] Preferably, the pest is a thrips;

[0134] Preferably, the plant disease includes at least one of damping-off, Fusarium wilt, Fusarium head blight, root rot, stem base rot, anthracnose, rice blast, bacterial blight, and citrus canker;

[0135] Preferably, the plant is selected from food crops, cash crops, or root and tuber crops;

[0136] Preferably, the food crop is selected from at least one of cereal crops, tuber crops, and legume crops;

[0137] Preferably, the cereal crops include at least one of the following grasses: rice, wheat, corn, barley, oats, rye, sorghum, millet, foxtail millet, barnyard millet, and buckwheat; preferably, the tuber crops include at least one of the following: sweet potato, yam, and potato; preferably, the legume crops include at least one of the following: soybean, broad bean, pea, mung bean, and peanut.

[0138] Preferably, the economic crop includes at least one of the following: plants of the Solanaceae family, Rosaceae family, Rutaceae family, Musaceae family, Cucurbitaceae family, Brassicaceae family, Orchidaceae family, Fabaceae family, Asteraceae family, Liliaceae family, Zingiberaceae family, Passifloraceae family, Bromeliaceae family, Araliaceae family, and Cactaceae family;

[0139] Preferably, the Solanaceae plant includes at least one of the following: tomato, pepper, potato, eggplant; preferably, the Rosaceae plant includes at least one of the following: strawberry, papaya; preferably, the Rutaceae plant includes citrus; preferably, the Musaceae plant includes banana; preferably, the Cucurbitaceae plant includes at least one of the following: cucumber, winter melon, pumpkin, bitter melon, loofah, watermelon, monk fruit; preferably, the Cruciferae plant includes at least one of the following: Chinese cabbage, rapeseed, kale, radish, cauliflower; preferably, the Orchidaceae plant includes orchid; preferably, the Fabaceae plant includes soybean; preferably, the Asteraceae plant includes lettuce; preferably, the Liliaceae plant includes garlic; preferably, the Zingiberaceae plant includes ginger; preferably, the Passifloraceae plant includes passion fruit; preferably, the Bromeliaceae plant includes pineapple; preferably, the Araliaceae plant includes Panax notoginseng; preferably, the Cactaceae plant includes dragon fruit.

[0140] Preferably, the root and tuber crops include at least one of the following: potatoes, carrots, leafy vegetables, solanaceous vegetables, strawberries, grapes, citrus fruits, bananas, kiwifruit, dragon fruit, tomatoes, peppers, beans, ginger, Panax notoginseng, and ginseng.

[0141] In some embodiments, the plant diseases and pests include plant replanting diseases, plant bacterial diseases, plant fungal diseases, plant viral diseases, soil-borne plant diseases, plant insect pests, and / or plant oomycete diseases. In other embodiments, the plant diseases and pests are caused by one or more of plant bacterial pathogens, plant fungal pathogens, viruses, insects, insect eggs, and nematodes.

[0142] In some embodiments, the aforementioned plant diseases and pests include, but are not limited to, at least one of scab, bacterial wilt, damping-off, black shank, and black spot.

[0143] In some embodiments, the nematodes include, but are not limited to, one or more of root-knot nematodes (Meloidogyne spp.) and *Caenorhabditis elegans*. In other embodiments, the root-knot nematodes include, but are not limited to, one or more of southern root-knot nematodes (M. incognita), northern root-knot nematodes (M. hapla), peanut root-knot nematodes (M. arenaria), and Javan root-knot nematodes (M. javanica).

[0144] In some embodiments, the aforementioned insects include, but are not limited to, insects belonging to the subclass Pterygota, such as Orthoptera, Isoptera (e.g., termites), Hemiptera (e.g., bugs), Homoptera, Thysanoptera (e.g., thrips), Coleoptera (e.g., beetles), Lepidoptera (e.g., moths or butterflies), Diptera, and Hymenoptera (e.g., bees).

[0145] In other embodiments, the aforementioned plant diseases and pests may also include those caused by mites. These mites include, but are not limited to, mites belonging to the families Tetranychidae, Amanidae, Erythropodidae, and Flouridae within the order Acari.

[0146] The plants described herein include plants at various stages, including those in the seed germination stage, seedling growth stage, robust seedling development stage, mature seedling flowering stage, and seed formation stage. Therefore, the plants described herein include both seedlings and fully grown plants. Furthermore, the plants described herein also include plant tissues and plant organs. Preferably, the plant tissues include, but are not limited to, meristematic tissues, protective tissues, ground tissues, and vascular tissues. Preferably, the plant organs include, but are not limited to, roots, stems, leaves, flowers, fruits, and seeds.

[0147] The following examples involve some of the culture media:

[0148] Gao's No. 1 agar plate medium: 20 g / L soluble starch, 1 g / L KNO3, 0.5 g / L NaCl, 0.5 g / L K2HPO4·3H2O, 0.5 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 20 g / L agar powder, pH = 7.2-7.4.

[0149] ISP2 solid medium: 4 g / L yeast extract, 10 g / L malt extract, 4 g / L glucose, 20 g / L agar powder, pH = 7.2-7.4.

[0150] Culture medium No. 1 (20 g / L soluble starch; 10 g / L soybean flour; 0.5 g / L MgSO4·7H2O; 0.5 g / L NaCl; 0.5 g / L K2HPO4; 0.01 g / L FeSO4·7H2O).

[0151] TTC agar plates: glucose 5 g / L, bacteriological peptone 10 g / L, acid-hydrolyzed casein 1 g / L, 2,3,5-triphenyltetrazolium chloride 0.05 g / L, agar 15 g / L, pH 7.2-7.4, autoclaved at 121°C for 20 minutes (from Huankai Microbiology, 022020P1).

[0152] SPA medium plates: sucrose 10g / L, bacteriological peptone 10g / L, agar powder 15g / L, agar 15g / L, pH=7.2-7.4, autoclave at 121℃ for 20 minutes.

[0153] LB medium: 10g peptone, 5g yeast extract, 5g sodium chloride, 1g glucose, add water to 1L, sterilize at 121℃ for 20min.

[0154] NA (Nutrient Agar) agar plate: 3g beef extract, 5g peptone, 2.5g glucose, 15g agar, 1L distilled water, sterilized at 121℃ for 20min.

[0155] V8 agar plate: 200ml V8 juice, 3g calcium carbonate, 800ml pure water, 15g agar, pH=7.2.

[0156] MKB iron-free medium: 5g casein amino acids, 15mL glycerol, 2.5g magnesium sulfate heptahydrate, 1000mL ultrapure water, pH 7.2, autoclaved at 121℃ for 20 minutes.

[0157] PDA culture medium plates (purchased from Guangdong Huankai)

[0158] The above-mentioned culture medium can be prepared using conventional preparation methods and sterilization methods.

[0159] The above implementation methods will be described below with reference to specific examples.

[0160] Example 1

[0161] Isolation and Identification of Strain MN327951

[0162] 1. Isolation of strain MN327951

[0163] The strain MN327951 involved in this invention was isolated from a soil sample from Zhuhai City, Guangdong Province.

[0164] Collect 5-10 grams of root zone soil, seal it in a self-sealing bag, and store it at low temperature in a sample storage box with ice packs.

[0165] Dispense physiological saline in the biosafety cabinet, 9 ml / tube;

[0166] Prepare the culture medium for strain isolation: Gao's No. 1 agar plate. Mark the sample information, culture medium type, and isolation date on the back of the plate.

[0167] Weigh 2g of soil into a 50mL Erlenmeyer flask, dry heat treat at 80-120℃ for 1h, add 18mL of 0.1% sodium pyrophosphate solution, place on a shaker and shake for 60min (150rpm), then treat with a 160W ultrasonic cleaner for 40s to obtain 10 -1 Soil dilution solution: Take 1 mL of soil dilution solution and add it to 9 mL of physiological saline solution. Mix well to obtain 10. -2 The diluent was then serially diluted to obtain 10. -3 Diluent, 10 -4 Diluent.

[0168] Take 10 -3 Diluent, 10-4 Add the diluent dropwise to Gao's No. 1 agar plates at a rate of 100 μL / plate, spread evenly, and after the surface of the Gao's No. 1 agar plates has dried, invert the Gao's No. 1 agar plates and incubate at 30°C for 7-14 days.

[0169] Observe the growth of the strains on Gao's No. 1 agar plates and pick single colonies with sterile toothpicks to purify the strains. The purified strains are then placed at 28°C for aerobic culture.

[0170] The strains that have undergone systematic screening were transferred to Gao's No. 1 agar plates and cultured aerobically at 28°C. The pure culture strains were then prepared into 20% glycerol / physiological saline-cell culture and stored at -86°C.

[0171] 2. Identification of strain MN327951

[0172] 2.1 Morphological characteristics

[0173] Figure 1 A photograph of the colony morphology of strain MN327951 is shown.

[0174] Figure 2 A microscopic morphological photograph of strain MN327951 is shown.

[0175] Figure 3 The Gram staining results of strain MN327951 are shown.

[0176] Strains MN327951 were inoculated onto ISP2 plates and cultured aerobically at 30°C for 168 hours. Visible colonies formed on the plates, producing yellowish-white aerial hyphae on ISP2 medium. Substrate hyphae were orange-yellow, and no soluble pigments were observed. The colonies were rugged, with fluffy aerial hyphae exhibiting concentric rings.

[0177] The strain was Gram-positive.

[0178] Microscopic morphological observation revealed that the spore filaments were long, wavy, and cumulated; the mature mycelium was highly branched, and both aerial and substrate hyphae were broken. Strain MN327951 conforms to the typical morphological characteristics of strains of the genus Streptomyces manipurensis.

[0179] The physiological and biochemical characteristics of the strain were tested using API 20E reagent strips (API 20E). Specific experimental procedures are detailed in the API reagent operation instructions. Culture conditions: 37℃, aerobic. The API 20E test results for strain MN327951 are shown in Table 1.

[0180] Table 1

[0181]

[0182]

[0183] 2.2 16S rRNA gene sequence sequencing

[0184] The 16S rRNA gene sequence of this strain was determined (the amplification primers and sequencing primers were 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-GGTTACCTTGTTACGACTT-3'), and the results are shown in SEQ ID No. 1 of the sequence listing.

[0185] The 16S rRNA gene sequence of strain MN327951 is as follows:

[0186] CAAGTCGAACGATGAAGCCCTTCGGGGTGGATTAGTGGCGAACGGGTGAGTAACA

[0187] CGTGGGCAATCTGCCCTTCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGAT

[0188] ACCACTCCTGTCCGCATGGGCAGGGGTTGAAAGCTCCGGCGGTGAAGGATGAGCCCGC

[0189] GGCCTATCAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGACGGGTAGCCGGCC

[0190] TGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGC

[0191] AGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGG

[0192] ATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTAC

[0193] CTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCA

[0194] AGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGT

[0195] GAAAGCCCGAGGCTTAACCTCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAG

[0196] GGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGT

[0197] GGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGA

[0198] ACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTTGGCGAC

[0199] ATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCC

[0200] GCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGC

[0201] TTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATATACCGGAAAGCATTAG

[0202] AGATAGTGCCCCCCTTGTGGTCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTC

[0203] GTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTGTGTTGCCAGCATG

[0204] CCCTTCGGGGTGATGGGGACTCACAGGAGACCGCCGGGGTCAACTCGGAGGAAGGTGG

[0205] GGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCC

[0206] GGTACAATGAGCTGCGATACCGTGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTC

[0207] GGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGC

[0208] ATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGT

[0209] CGGTAACACCCGAAGCCGGTGGCCCAACCCTTGTGGAGGGA (SEQ ID No. 1).

[0210] Through BLAST homology comparison, the strain was identified as Streptomyces manipurensis and named Streptomyces manipurensis MN327951.

[0211] Figure 4 A schematic diagram of the phylogenetic tree of strain MN327951 is shown.

[0212] like Figure 4 As shown, the phylogenetic tree results are as follows: Figure 4 As shown, strain MN327951 is closest to Streptomyces manipurensis. Based on morphological characteristics, this indicates that the strain belongs to Streptomyces manipurensis.

[0213] Example 2

[0214] Antibacterial activity assay of strain MN327951

[0215] 1. Test strains

[0216] The tested plant pathogenic fungi were: Phytophthora sojae, Colletotrichumsiamense, Fusarium graminearum, and Rhizoctonia solani.

[0217] The tested plant pathogenic bacteria were: Ralstonia solanacearum, Xanthomonas oryzae pv oryzae, Pectobacterium carotovorum subsp. carotovorum, and Pseudomonas syringae pv. tomato.

[0218] 2. Activation of the strain

[0219] Activation of strain MN327951: Take the bacterial suspension of strain MN327951 preserved in glycerol in Example 1 and streak it on the surface of ISP2 medium plate.

[0220] Activation of plant pathogenic fungi: Plant pathogenic fungi agar blocks preserved in glycerol were inoculated onto the surface of the corresponding culture medium plates. Phytophthora soybeana was activated on V8 agar plates, while anthracnose fungi, Fusarium graminearum, and Rhizoctonia solani were activated on PDA agar plates.

[0221] Activation of plant pathogenic bacteria: Streaking of glycerol-preserved plant pathogenic bacteria suspensions onto the corresponding culture medium plates. Ralstonia solanacearum was activated on TTC medium plates, rice bacterial blight pathogens were activated on SPA medium plates, and carotene soft rot bacillus and syringomonas pathogenic tomato pathogens were activated on NA medium plates (purchased from Guangdong Huankai).

[0222] 3. Preparation of plant pathogenic bacteria inoculum

[0223] Plant pathogenic bacteria were cultured on culture plates for 2-4 days. Single colonies were picked up with an inoculation loop and inoculated into 10 ml of liquid culture medium (Ralstonia solanacearum was inoculated into TTC medium, Bacillus oryzae of rice was inoculated into SPA medium, and Bacillus carotene soft rot and Pseudomonas syringae, a pathogenic species of tomato, were inoculated into LB medium). The culture was carried out at 28°C with shaking at 200 rpm for 24 hours.

[0224] 4. Preparation of plant pathogenic fungal cakes

[0225] After activation, the plates that have grown to fill 90mm round culture dishes are used to prepare mycelial cakes by punching holes along the outer 1 / 3 of the colony.

[0226] 5. Preparation of mycelial cakes from strain MN327951

[0227] Use a sterile toothpick to pick up MN327951 mycelial growth and streak it densely on an ISP2 medium plate (to make the plate densely distributed with colonies). Incubate at 28°C for 3-5 days, and prepare mycelial cakes using a 7mm diameter punch.

[0228] 6. Plate confrontation of strain MN327951 against plant pathogenic fungi

[0229] A mycelial disc of the plant pathogenic fungus was inoculated in the center of a blank culture medium plate. A mycelial disc of colony MN327951 was inoculated at the midpoint of the plate's radius, allowing the pathogenic fungus and strain MN327951 to culture on the same plate. (Phytophthora soybeanis was cultured on V8 medium plates, while anthracnose fungi, Fusarium graminearum, and Rhizoctonia solani were cultured on PDA medium plates.)

[0230] 7. Plate confrontation of strain MN327951 against plant pathogenic bacteria

[0231] Take 100 μl of plant pathogenic bacteria suspension and spread it on the surface of NA medium plate. Inoculate the bacterial cake of strain MN327951 at the midpoint of the plate radius and let the pathogenic bacteria and strain MN327951 be cultured on the same plate.

[0232] 8. Statistics on the results of the standoff

[0233] Inhibition rate (%) of plant pathogenic fungi = (Coronation radius of pathogenic fungi in negative control group - Colony radius of pathogenic fungi in treatment group) / Colony radius of pathogenic fungi in negative control group × 100

[0234] The width of the inhibition zone of plant pathogenic bacteria = the radius of the transparent inhibition zone - the radius of the antagonistic bacteria colony

[0235] Figure 5 The diagram shows the antibacterial effect of strain MN327951 against different plant pathogenic fungi.

[0236] Table 2 shows the statistical data on the antibacterial effects of strain MN327951 against different plant pathogenic fungi.

[0237] Table 2

[0238] Pathogen Name Antibacterial rate (%) Soybean Phytophthora 45.45 Anthrax bacteria 17.33 Fusarium graminearum 32.00 Rhizoctonia solani 13.33

[0239] Figure 6 The diagram shows the antibacterial effect of strain MN327951 against different plant pathogenic bacteria.

[0240] Table 3 shows the statistical data on the antibacterial effects of strain MN327951 against different plant pathogenic bacteria.

[0241] Table 3

[0242] Pathogen Name Antagonism Index bacterial wilt 1.39 Rice bacterial blight fungus 2.56 Carotene soft rot bacteria 1.33 Pseudomonas syringae pathogenic strain in tomato 2.17

[0243] Strain MN327951 exhibits strong and broad-spectrum antibacterial activity against a variety of pathogens.

[0244] Example 3

[0245] Determination of the potted plant efficacy of spore suspension of strain MN327951 in preventing bacterial wilt of tomato

[0246] 1. Preparation of fermentation broth for strain MN327951

[0247] The strain MN327951, which was activated on the ISP2 medium plate in Example 2, was transferred to medium 1 and cultured in a shaker at 30°C and 200 rpm for 6 days to obtain the fermentation broth of strain MN327951.

[0248] 2. Water potted tomatoes with MN327951 fermented liquid

[0249] Two-week-old tomato seedlings were selected, one seedling per pot, and each pot was watered with 50ml of MN327951 fermentation liquid diluted 10 times. Nine pots were used as one group, with three replicates. Tomato seedlings watered with plain water were used as a negative control.

[0250] 3. Inoculate tomatoes with Tomato Bacterial Wilt Bacterium.

[0251] Tomato potted plants were treated with MN327951 fermentation broth for 7 days and then inoculated with Tomato Bacterium wilt.

[0252] The activated bacterial wilt pathogen (Ralstonia solanacearum) from Example 2 was inoculated into 200 ml of TTC liquid medium and cultured at 30°C with shaking at 200 rpm for 20 hours. The cultured bacterial wilt pathogen suspension was then diluted to OD200. 600 =0.01, water 50ml per pot.

[0253] From the first sign of symptoms (bacterial wilt in tomato seedlings) (generally, the disease begins to appear 3-4 days after root irrigation), observe and record the condition of tomato seedlings every 1 or 2 days (record the disease situation according to the grading standard) until day 7-13 (determined according to the actual disease situation), record the number of plants for each disease level in each treatment, and calculate the incidence rate and disease index according to the following formula.

[0254] The disease severity of plants is classified into the following 5 levels:

[0255] Level 0: Asymptomatic

[0256] Level 1: One leaf withered

[0257] Grade 2: 2-3 leaves wilted

[0258] Level 3: The entire plant withers

[0259] Level 4: Plant dead

[0260] Disease index (%) = (∑(number of plants at each disease level × number of levels) / (total number of plants × highest level)) × 100%

[0261] Prevention and control efficacy (%) = ((Disease index of negative control group - Disease index of experimental group) / Disease index of negative control group) × 100%

[0262] Table 4 shows the statistical data on the preventive effect of fermentation broth of strain MN327951 on bacterial wilt of tomato.

[0263] Table 4

[0264] deal with Relative efficacy (%) negative control / 10-fold dilution of fermentation broth of MN327951 33.82

[0265] Fermentation broth of strain MN327951 has a good preventive effect against bacterial wilt of tomatoes.

[0266] Example 4

[0267] The control efficacy of fermentation broth from strain MN327951 against western flower thrips

[0268] 1. Preparation of fermentation broth for strain MN327951

[0269] Three mycelial discs were formed from the grown strain MN327951 on a plate and inoculated into 200 ml of Gao's No. 1 liquid culture medium. The culture was carried out at 30°C and 200 rpm with shaking for 5 days.

[0270] 2. Virulence determination of fermentation broth supernatant of strain MN327951 against western flower thrips

[0271] After centrifuging the fermentation broth of strain MN327951, the supernatant was used for virulence determination.

[0272] The toxic activity of the tested fermentation broth against western flower thrips was tested using the immersion method.

[0273] Twenty adult western flower thrips were inoculated into 5 mL pipette tips after sterilization, with three replicates per treatment. The 5 mL pipette tips containing the thrips were used to immerse the thrips in the supernatant of the fermentation broth for 10 seconds in each treatment group. A blank culture medium was used as a negative control. The treated western flower thrips were then kept in a rearing room with a 12h:12h light cycle, a temperature of 25±2℃, and a relative humidity of 55%. Honey was added to the thrips' diet every two days, and the number of dead thrips was counted on the fourth day.

[0274] Mortality rate (%) = (Number of dead insects / Number of test insects) × 100%

[0275] Table 5 shows the statistical data of the toxicity test results of the fermentation broth supernatant of strain MN327951 against western flower thrips.

[0276] Table 5

[0277] deal with Mortality rate (%) after 4 days supernatant of fermentation broth of strain MN327951 10 negative control 0

[0278] The supernatant of the fermentation broth of strain MN327951 has strong toxicity to western flower thrips and is suitable for use as an effective ingredient in bacterial agents.

[0279] Example 5

[0280] Determination of the ability of strain MN327951 to produce siderophores

[0281] 1. Iron starvation treatment: Activate strain MN327951 on ISP2 plates. Take three 7mm diameter bacterial discs using a punch and inoculate them into 10mL of MKB iron-free medium (5g casein amino acids, 15mL glycerol, 2.5g magnesium sulfate heptahydrate, 1000mL ultrapure water, pH 7.2, autoclaved at 121℃ for 20min; 5% dipotassium hydrogen phosphate solution, autoclaved at 121℃ for 20min; add potassium dihydrogen phosphate solution to the medium to make the working concentration 0.25%). Incubate at 30℃ with shaking at 200rpm for 72h. Centrifuge to collect 1mL of bacterial cells, wash the cells three times with sterile water, and resuspend in 1mL of sterile water to obtain a bacterial suspension.

[0282] 2. CAS Plate Covering Method for Detecting Siderophores: Inoculate 10 μL of bacterial suspension in 3 drops onto an iron-free MKB plate and incubate at 30℃ for 1-3 days. After clear single colonies have grown at all 3 inoculation sites on the plate, pour a layer of CAS detection medium (melted and cooled to 60℃, purchased from Qingdao Haibo Biotechnology, sterilized at 116℃ for 30 min) onto the plate covering the colonies. Incubate the plate covered with the CAS detection medium at 30℃ and continuously observe the color change.

[0283] 3. Results Measurement and Analysis: Measure the diameter of the siderophore chelation halo and the colony diameter, and calculate the siderophore solubility index (siderophore solubility index = siderophore chelation halo diameter / colony diameter).

[0284] Figure 7 A schematic diagram shows the formation of siderophore chelate rings by strain MN327951 on a CAS detection plate.

[0285] Table 6 shows the statistical data on the ability of strain MN327951 to produce siderophores.

[0286] Table 6

[0287] strain name Diameter of the ferrocarrier chelating ring (mm) Ferric carrier soluble MN327951 16.67 1.70

[0288] Strain MN327951 has siderophore activity, which can promote plant growth by promoting the absorption of nutrients, especially iron ions.

[0289] The present application has been described in detail above with reference to the embodiments. However, the present application is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present application. Furthermore, unless otherwise specified, the embodiments and features in the embodiments of the present application can be combined with each other.

Claims

1. A strain of Streptomyces mannipur, characterized in that, Choose from any one of A1) to A3): A1) The strain is deposited at the Guangdong Provincial Center for Microbial Culture Collection, with accession number GDMCC No. 64195; A2) Strains whose 16S rRNA gene sequence has at least 98.65%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, or 100% identity with the sequence shown in SEQ ID No. 1; A3) Strains whose genome sequence has an average nucleotide identity of at least 95%, 96%, 97%, 98%, 99%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, 99.99%, or 100% with A1), and / or strains whose genome sequence has an alignment score of ≥60%, ≥65%, ≥70%, ≥78%, ≥80%, ≥85%, ≥90%, ≥95%, ≥96%, ≥97%, ≥98%, or ≥99% with A1).

2. The culture of the *Streptomyces manipur* strain according to claim 1, characterized in that, Includes any one of B1) to B5): B1) Fermentation medium for the Streptomyces manipur strain; B2) Fermentation supernatant of the Streptomyces manipur strain described above; B3) The concentrated product of B1) or B2); B4) The extract of B1) or B2); B5) The dried product of any one of B1) to B4); Preferably, the fermentation medium is a liquid culture medium; Preferably, the culture temperature is 28–30°C, and / or the culture time is 7–14 days.

3. A composition, characterized in that, It includes at least the Streptomyces manipurense strain as described in claim 1 and / or any one of its metabolites, cultures, fermentation broths, or extracts. Preferably, the composition further comprises additional surfactants and / or excipients; Preferably, the adjuvant is selected from one or more other biological control agents, one or more other microbial control agents, one or more chemical agents, one or more fertilizers, one or more herbicides, one or more growth promoters, or any combination thereof; Preferably, the excipients are selected from agriculturally or horticulturally acceptable carriers, diluents, stabilizers, fillers, wetting agents, colorants, solvents, co-solvents, film-forming agents, spontaneous growth promoters, emulsifiers, dispersants, preservatives, antifreeze agents, thickeners, adjuvants, or any combination thereof; Preferably, the one or more additional biocontrol agents are selected from bacteria, fungi, viruses, actinomycete biocontrol agents, insect biocontrol agents, oviposition and worm biocontrol agents, nematode biocontrol agents, or any combination thereof; Preferably, the one or more additional microbial control agents are selected from strains of Trichoderma, Bacillus, or any combination thereof, or from metabolites of Trichoderma, Bacillus, or any combination thereof, or from fermentation broth or fermentation supernatant of Trichoderma, Bacillus, or any combination thereof. Preferably, the bacteria of the genus Bacillus are selected from: Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans, Paenibacillus polymyxa, Bacillus myloliquefaciens, or any combination thereof; Preferably, the one or more additional biocontrol agents are selected from at least one of Trichoderma harzianum, Purpureocillium lilacinum, Penicillium bilaiae, Bacillus subtilis, Burkholderia rinojensis, Bacillus pumilus, Bacillus velezensis, Methylobacterium rhodesianum, Methylobacterium extorquens, and Paenibacillus peoriae. Preferably, the *Trichoderma harzianum* is *Trichoderma harzianum* with accession number CGMCC No. 15679; Preferably, the *Paecilomyces lilacinus* is *Paecilomyces lilacinus* with accession number CGMCC No. 12773; Preferably, the Penicillium baqensis is Penicillium baqensis with accession number CGMCC No. 12767; Preferably, the Bacillus subtilis is Bacillus subtilis with accession number CGMCC No. 12908; Preferably, the *Rhodotorula buergerianum* is *Rhodotorula buergerianum* with accession number GDMCC No. 61156; Preferably, the *Bacillus pumilus* is the *Bacillus pumilus* with accession number GDMCC No. 61962; Preferably, the Bacillus belyssus is Bacillus belyssus with accession number GDMCC No. 61434; Preferably, the *Methylobacterium roximatee* is *Methylobacterium roximatee* with accession number GDMCC No. 60729; Preferably, the *Methylobacterium tumefaciens* is *Methylobacterium tumefaciens* with accession number GDMCC No. 62943; Preferably, the *Bacillus pilaris* is *Bacillus pilaris* with accession number GDMCC No. 60482; Preferably, the composition is in solid form, liquid form, powder form, or any combination thereof; Preferably, the composition is selected from at least one of solutions, powders, granules, emulsions, emulsions, suspensions, tablets, microparticles, and wettable powders.

4. A microbial inoculant, characterized in that, It includes at least one of the following: the *Streptomyces manipur* strain and / or its metabolites as described in claim 1, the culture as described in claim 2, and the composition as described in claim 3. Preferably, the microbial agent further comprises agriculturally or horticulturally acceptable diluents, fillers, solvents, accelerators, carriers, emulsifiers, dispersants, preservatives, antifreeze agents, thickeners, adjuvants, or any combination thereof; Preferably, the microbial agent is a powder, granules, or liquid.

5. A microbial insecticide, microbial fertilizer, microbial pesticide, or biostimulant, characterized in that, It includes at least one of the following: the Streptomyces manipurii strain and / or its metabolites as described in claim 1, the culture as described in claim 2, and the composition as described in claim 3.

6. A plant growth regulator, seed coating agent, root drenching agent, seed soaking agent, microbial pesticide, or microbial fertilizer, characterized in that, It includes at least one of the following: the Streptomyces manipurii strain and / or its metabolites as described in claim 1, the culture as described in claim 2, and the composition as described in claim 3.

7. The use of the *Streptomyces manipur* strain of claim 1, the culture of claim 2, the composition of claim 3, the microbial agent of claim 4, the microbial insecticide or microbial fertilizer or microbial pesticide or biostimulant of claim 5, or the plant growth regulator, seed coating agent, root drenching agent, seed soaking agent, microbial pesticide or microbial fertilizer of claim 6 in the preparation of antifungal products, antibacterial products, seed coating products, biofertilizers, root drenching agents, seed soaking agents, microbial pesticides or microbial fertilizers.

8. The application of the *Streptomyces manipur* strain of claim 1, the culture of claim 2, the composition of claim 3, the microbial agent of claim 4, the microbial insecticide or microbial fertilizer or microbial pesticide or biostimulant of claim 5, or the plant growth regulator, seed coating agent, root irrigation agent, seed soaking agent, microbial pesticide or microbial fertilizer of claim 6 in the prevention and control of plant diseases and pests; Preferably, the plant diseases and pests include infectious and / or non-infectious diseases and pests; Preferably, the plant diseases and pests include those caused by pathogenic bacteria, pathogenic fungi, nematodes, insect eggs and / or pests; Preferably, the pathogenic fungi include: At least one of the following pathogens: Colletotrichum, Rhizoctonia, Fusarium, Magnaporthe, and Phytophthora. Preferably, the pathogenic fungi include at least one of the following: Colletotrichum siamense, Rhizoctonia solani, Fusarium graminearum, Magnaphortheoryzae, and Phytophthora sojae; Preferably, the pathogenic bacteria include at least one of the following: Pectobacterium, Pseudomonas, Xanthomonas, and Ralstonia. Preferably, the pathogenic bacteria include at least one of the following: *Pectobacterium carotovorum subsp. carotovorum*, *Pseudomonas syringae pv. tomato*, *Xanthomonas axonopodis pv. citri*, *Ralstonia solanacearum*, and *Xanthomonas oryzae pv. oryzae*. Preferably, the pest is a thrips; Preferably, the plant disease includes at least one of damping-off, Fusarium wilt, Fusarium head blight, root rot, stem base rot, anthracnose, rice blast, bacterial blight, and citrus canker; Preferably, the plant is selected from food crops, cash crops, or root and tuber crops; Preferably, the food crop is selected from at least one of cereal crops, tuber crops, and legume crops; Preferably, the cereal crops include at least one of the following grasses: rice, wheat, corn, barley, oats, rye, sorghum, millet, foxtail millet, barnyard millet, and buckwheat; preferably, the tuber crops include at least one of the following: sweet potato, yam, and potato; preferably, the legume crops include at least one of the following: soybean, broad bean, pea, mung bean, and peanut. Preferably, the economic crop includes at least one of the following: plants of the Solanaceae family, Rosaceae family, Rutaceae family, Musaceae family, Cucurbitaceae family, Brassicaceae family, Orchidaceae family, Fabaceae family, Asteraceae family, Liliaceae family, Zingiberaceae family, Passifloraceae family, Bromeliaceae family, Araliaceae family, and Cactaceae family; Preferably, the Solanaceae plant includes at least one of the following: tomato, pepper, potato, eggplant; preferably, the Rosaceae plant includes at least one of the following: strawberry, papaya; preferably, the Rutaceae plant includes citrus; preferably, the Musaceae plant includes banana; preferably, the Cucurbitaceae plant includes at least one of the following: cucumber, winter melon, pumpkin, bitter melon, loofah, watermelon, monk fruit; preferably, the Cruciferae plant includes at least one of the following: Chinese cabbage, rapeseed, kale, radish, cauliflower; preferably, the Orchidaceae plant includes orchid; preferably, the Fabaceae plant includes soybean; preferably, the Asteraceae plant includes lettuce; preferably, the Liliaceae plant includes garlic; preferably, the Zingiberaceae plant includes ginger; preferably, the Passifloraceae plant includes passion fruit; preferably, the Bromeliaceae plant includes pineapple; preferably, the Araliaceae plant includes Panax notoginseng; preferably, the Cactaceae plant includes dragon fruit. Preferably, the root and tuber crops include at least one of the following: potatoes, carrots, leafy vegetables, solanaceous vegetables, strawberries, grapes, citrus fruits, bananas, kiwifruit, dragon fruit, tomatoes, peppers, beans, ginger, Panax notoginseng, and ginseng.

9. The application of the *Streptomyces manipur* strain of claim 1, the culture of claim 2, the composition of claim 3, the microbial agent of claim 4, the microbial insecticide, microbial fertilizer, microbial pesticide, or biostimulant of claim 5, or the plant growth regulator, seed coating agent, root drenching agent, seed soaking agent, microbial pesticide, or microbial fertilizer of claim 6 in promoting plant growth. Preferably, the promotion of plant growth includes: Promotes the absorption of nutrients by plants. Preferably, promoting plant growth includes promoting the absorption of iron ions by the plant.

10. A method for preventing and controlling plant diseases and pests, improving crop resistance to adverse conditions, and / or promoting plant growth, characterized in that, The method includes applying to plants or seeds the Streptomyces manipurense strain of claim 1, the culture of claim 2, the composition of claim 3, the microbial agent of claim 4, the microbial insecticide or microbial fertilizer or microbial pesticide or biostimulant of claim 5, or the plant growth regulator of claim 6, seed coating agent, root irrigation agent, seed soaking agent, microbial pesticide or microbial fertilizer.

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