Method for preparing radix rehmanniae recen decoction pieces from radix stephaniae tetrandrae and application thereof
By preparing Fangji-processed Rehmannia glutinosa slices, the problem of unstable medicinal properties in the synergistic effect of Rehmannia glutinosa and Fangji was solved, and a new raw material with unified medicinal properties was achieved. This enhanced the anti-inflammatory, analgesic, and antidepressant effects, and provided a highly effective treatment plan for comorbid rheumatism and depression.
Patent Information
- Application Number
- CN202511805651.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-03
- Publication Date
- 2026-01-16
AI Technical Summary
In existing technologies, the synergistic effect of Rehmannia glutinosa and Stephania tetrandra can only be achieved through temporary decoction, lacking systematic activation and enhancement of their specificity in the treatment of rheumatic depression comorbidities, and lacking new raw materials with unified medicinal properties.
By mixing Rehmannia glutinosa and Stephania tetrandra slices in a specific ratio, soaking them in Stephania tetrandra decoction and stir-frying them, Stephania tetrandra-processed Rehmannia glutinosa slices are prepared, ensuring that the characteristic components of Rehmannia glutinosa and Stephania tetrandra are deeply integrated in terms of medicinal properties.
The prepared Fangji-processed Rehmannia glutinosa slices achieve a deep integration of "dispelling wind and dampness" and "nourishing yin and blood, calming the mind" in terms of medicinal properties, significantly enhancing anti-inflammatory, analgesic and antidepressant effects, and providing highly effective treatment support for rheumatic depression comorbidity.
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Abstract
Description
TECHNICAL FIELD
[0001] The application relates to the field of processing of traditional Chinese medicinal materials, and particularly relates to a method for processing radix aconiti ruzickii and radix rehmanniae preparata and application thereof. BACKGROUND
[0002] Radix rehmanniae preparata is cold in nature and is good at clearing heat and blood and nourishing yin and generating fluid and is a main medicinal herb for nourishing yin and blood. Although there is a record of radix rehmanniae preparata "expelling blood stasis" in ancient books such as Shennong's Classic of Materia Medica, which implies that radix rehmanniae preparata has the potential of relieving arthralgia, in later clinical practice and the current Chinese Pharmacopoeia and mainstream Chinese materia medica textbooks, the core effect of radix rehmanniae preparata has been focused on "clearing heat and blood and nourishing yin and generating fluid", and the effect of "expelling wind and dampness" has been seriously marginalized and is not the core indication. Rheumatic diseases belong to the category of "bi syndrome" in traditional Chinese medicine, and the disease course is prolonged, "prolonged disease must be deficiency", "prolonged disease affects the kidney", and "prolonged pain enters the collaterals" and "prolonged pain must be blood stasis", leading to complex conditions. Clinically, the common "deficiency of yin and blood deficiency" syndrome is the root and the wind, dampness and blood stasis blocking the meridians is the branch. More difficultly, long-term joint pain, limited activity and worry about prognosis can easily lead to depression, forming "rheumatic depression comorbidity", a refractory disease, which increases the difficulty of clinical treatment.
[0003] For the complex conditions of rheumatic syndrome or comorbid depression, radix rehmanniae preparata alone has no obvious effect. Radix aconiti ruzickii is bitter and acrid and cold in nature and is good at expelling wind and relieving pain, reducing water and swelling and is a main medicinal herb for treating rheumatic arthralgia. In Zhang Zhongjing's Decoction of Radix Aconiti Ruzickii and Radix Rehmanniae (radix aconiti ruzickii 7.5g, cassia twig 22.5g, siler 22.5g, and radix rehmanniae 60-120g), radix aconiti ruzickii and radix rehmanniae preparata are combined, the combined weight is radix aconiti ruzickii: radix rehmanniae preparata = 1:8-16, radix rehmanniae preparata nourishes yin and blood, radix aconiti ruzickii expels wind and dredges collaterals, and the decoction is mainly used for treating "disease like a madman, wandering, and talking non-stop", which is related to wind and dampness entering blood and heat disturbing the spirit, indicating the application potential of the medicine in "wind and dampness disturbing the spirit".
[0004] However, there is a common understanding and technical bias in the field that the synergistic effect of rehmannia and stephania can only be achieved through the clinical compatibility of “Fangji Dihuang Decoction”. This compatibility at the prescription level is a transient interaction between the two independent medicinal materials during decoction, which is unstable in material basis and requires temporary prescription each time. Currently, the processing of rehmannia mainly focuses on steaming and stewing to change the medicinal property (such as making mature rehmannia), or there are authorized patents involving processing with lily juice and other anshen drugs to enhance its ziny and anshen effects. However, there is no report on processing with stephania juice, a drug for removing wind and dampness, to systematically activate and enhance the potential of rehmannia in removing arthralgia, and to create an integrated new decoction piece for the treatment of rheumatism and depression comorbidity. Therefore, how to “solidify” the synergistic effect of rehmannia and stephania in a single decoction piece through processing, create a new raw material with unified medicinal property, clear material basis, and direct clinical prescription, to solve the technical problem of lack of targeted and efficient drugs for rheumatism and depression comorbidity, has become the core problem to be solved by the present application. SUMMARY
[0005] In view of the above, in order to overcome the shortcomings of the prior art, the purpose of the present application is to provide a method for processing stephania-prepared rehmannia decoction pieces and its application, which can effectively process rehmannia to solve the problem of lack of targeted and efficient drugs for rheumatism and depression comorbidity.
[0006] The technical solution solved by the present application is: a method for processing stephania-prepared rehmannia decoction pieces, comprising the following steps: S1, taking rehmannia decoction pieces and cleaning; S2, taking stephania decoction pieces, decocting 2 times with 10 times the weight volume of water each time, 30 minutes each time, combining the filtrate, and concentrating to a stephania decoction liquid with a mass of 0.33-0.67 g / mL; the weight volume refers to g for solid and mL for liquid; S3, mixing the rehmannia decoction pieces with the stephania decoction liquid in a proportion of rehmannia to stephania raw medicinal materials at a mass ratio of 100:10-20 (i.e. 10:1-2), and soaking for 4 hours until the medicine is fully soaked in the juice; S4, placing the soaked rehmannia decoction pieces in a 70℃ oven and frying until the surface is dark brown and the cross section is black, and drying, to obtain the finished stephania-prepared rehmannia decoction pieces.
[0007] The application of the stephania-prepared rehmannia decoction pieces prepared by the above method in preparing drugs for treating rheumatism and depression comorbidity.
[0008] The application of the stephania-prepared rehmannia decoction pieces prepared by the above method in preparing anti-inflammatory, analgesic, joint swelling improving, and antidepressant drugs.
[0009] The preparation method is novel and unique, simple to operate, and first successfully prepares the prepared radix Stephaniae tetrandrae and radix Rehmanniae preparata with synergistic effects, realizes deep fusion of "dispelling wind and dampness (attacking evil)" and "nourishing yin and blood and calming the heart spirit (upholding the right)" in medicinal properties, surpasses simple superposition of original medicinal materials, becomes a new traditional Chinese medicine raw material with integrated effects, provides technical support for treating rheumatism and depression comorbidity, is a great innovation in the field of processing of traditional Chinese medicinal materials, and has practical popularization and application value. BRIEF DESCRIPTION OF DRAWINGS
[0010] Figure 1 A preparation process flowchart of the present application is shown in the following table. Figure 2 A comparison chart of HPLC characteristic chromatograms of the prepared products of examples 1-3 and radix Rehmanniae and radix Stephaniae tetrandrae is shown in the following table. DETAILED DESCRIPTION
[0011] The specific embodiments of the present application are described in detail below in combination with examples and specific cases.
[0012] The present application is given in the following examples in the specific implementation process: EXAMPLE
[0013] The method for preparing radix Stephaniae tetrandrae and radix Rehmanniae preparata of the present application comprises the following steps: S1, taking 100 g of radix Rehmanniae preparata, cleaning and preparing; S2, taking 20 g of radix Stephaniae tetrandrae, decocting 10 times the weight volume of water (200 mL) each time for 2 times, 30 minutes each time, combining the filtrates, and concentrating to 30 mL of radix Stephaniae tetrandrae water decoction with a mass of 0.67 g / mL; S3, uniformly mixing the radix Rehmanniae preparata with the radix Stephaniae tetrandrae water decoction, and soaking for 4 hours until the medicine is fully soaked with the juice; S4, placing the soaked radix Rehmanniae preparata at 70 DEG C under gentle frying until the surface is dark brown and the cross section is black, and drying, to obtain the radix Stephaniae tetrandrae and radix Rehmanniae preparata; S5, detection: the prepared product is a single traditional Chinese medicine entity containing two types of characteristic components of radix Rehmanniae and radix Stephaniae tetrandrae, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehderin D is not less than 3.5 mg / g, and both tetrandrine and fangchinoline are contained, which is the finished product. EXAMPLE
[0014] The method for preparing radix Stephaniae tetrandrae and radix Rehmanniae preparata of the present application comprises the following steps: S1, taking 100 g of radix Rehmanniae preparata, cleaning and preparing; S2, taking 20 g of radix Stephaniae tetrandrae, decocting 10 times the weight volume of water (200 mL) each time for 2 times, 30 minutes each time, combining the filtrates, and concentrating to 30 mL of radix Stephaniae tetrandrae water decoction with a mass of 0.67 g / mL; S3, mix the raw Rehmannia root slices with the water decoction of the said Stephania tetrandra, and soak for 4 hours until the medicine is fully soaked; S4, place the soaked raw Rehmannia root slices in a 70℃ oven and fry until the surface is dark brown and the cross section is black, and dry, to obtain the Stephania tetrandra processed Rehmannia root slices; S5, detection: the slices are single Chinese medicine entities containing two types of characteristic components of Rehmannia root and Stephania tetrandra, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehhygon D is not less than 3.5 mg / g, and both tetrandrine and fangchinoline are contained, which are the finished products. Embodiment
[0015] The method for preparing the Stephania tetrandra processed Rehmannia root slices comprises the following steps: S1, take 100g of raw Rehmannia root slices and clean them; S2, take 15g of Stephania tetrandra slices, and decoct 10 times the weight of water (200 mL) each time for 2 times, 30 minutes each time, combine the filtrates, and concentrate to 0.50g / mL of Stephania tetrandra water decoction; S3, mix the raw Rehmannia root slices with the water decoction of the said Stephania tetrandra, and soak for 4 hours until the medicine is fully soaked; S4, place the soaked raw Rehmannia root slices in a 70℃ oven and fry until the surface is dark brown and the cross section is black, and dry, to obtain the Stephania tetrandra processed Rehmannia root slices; S5, detection: the slices are single Chinese medicine entities containing two types of characteristic components of Rehmannia root and Stephania tetrandra, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehhygon D is not less than 3.5 mg / g, and both tetrandrine and fangchinoline are contained, which are the finished products.
[0016] The Stephania tetrandra processed Rehmannia root slices prepared by the above method are single Chinese medicine entities containing two types of characteristic components of Rehmannia root and Stephania tetrandra, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehhygon D is not less than 3.5 mg / g, and both tetrandrine (i.e. tetrandrine) and fangchinoline (i.e. fangchinoline) are contained.
[0017] The Stephania tetrandra processed Rehmannia root slices prepared by the above method are used for preparing a medicine for treating rheumatism, depression and other diseases.
[0018] The Stephania tetrandra processed Rehmannia root slices prepared by the above method are used for preparing an anti-inflammatory, analgesic, joint swelling improving and anti-depression medicine.
[0019] The Stephania tetrandra processed Rehmannia root slices prepared by the above method are used in combination with other Chinese medicine compositions.
[0020] The prepared Rehmannia glutinosa slices according to the above embodiments can be prepared in any quantity as needed. The embodiments given are only used to illustrate the specific implementation of the present invention, and are not intended to limit the scope of protection of the present invention. The core technology protected by the present invention is the preparation method of prepared Rehmannia glutinosa slices.
[0021] This invention presents a novel and unique preparation method that is simple to operate. It successfully prepares synergistically effective processed Rehmannia glutinosa slices containing both Rehmannia glutinosa and Stephania tetrandra (cattailol, rehmannia glycoside D, fangchinoline, and fangchinoline), while significantly altering the content of Rehmannia glutinosa's own components. This achieves a deep fusion of "dispelling wind and dampness (attacking pathogens)" and "nourishing yin and blood, calming the mind (maintaining righteousness)" in terms of medicinal properties, surpassing the simple superposition of the original medicinal materials and achieving excellent and beneficial technical effects. The experimental data are as follows: I. Model Establishment and Drug Administration Seventy male ICR mice were acclimatized for one week and then randomly divided into seven groups (n=10 per group) according to body weight: ① blank control group, ② model group, ③ Rehmannia glutinosa group, ④ Example 1 group, ⑤ Example 2 group, ⑥ Example 3 group, and ⑦ Stephania tetrandra group. A mouse model of rheumatic depression was established using bovine type II collagen induction combined with chronic unpredictable mild stress (CUMS) depression. Intervention combined with CUMS was initiated on the day of the initial immunization (two or more methods were randomly selected daily) and continued until the last day of administration (49 days). Drug intervention began after the second collagen immunization. Mice in the normal group received an equal volume of solvent at the same time point, while the other groups were administered the corresponding drug solution by gavage. The dosage was calculated based on the human-animal body surface area equivalent dose ratio: 2 g / kg of the corresponding raw herb in the Rehmannia glutinosa group and each example group. -1 Gavage administration: The Fangji group was administered Fangji raw herb at a dose of 1 g / kg. -1 Gavage.
[0022] II. Experiment 2.1 Experiment on the effect of improving the behavior of a comorbid rheumatic depression model Behavioral test results: The model group showed a significantly lower preference for sucrose water and a significantly longer immobility time during tail suspension and forced swimming. Compared with the model group and the Rehmannia glutinosa group, the sucrose water preference in Example 1 group (100:20) was significantly increased by 75.0%. P <0.01), the immobility time during tail suspension and forced swimming decreased significantly, with decrease rates of 40.9% and 33.2%, respectively. P <0.01), which is more effective than other ratio groups and single-drug groups.
[0023] Conclusion: The prepared Rehmannia glutinosa slices of the present invention, especially those prepared at the optimal ratio (100:20) protected by claim 1, produced an unexpected synergistic improvement effect on depressive-like behavior, as shown in Table 1: Table 1 Influence on mouse behavior
[0024]
[0025] Note: compared with the blank control group, * P <0.05, ** P <0.01; compared with the model group, # P <0.05, ## P <0.01; compared with the rehmannia group, & P <0.05, && P <0.01, same below.
[0026] 2.2, Wind-dampness and analgesic pharmacological experiments Anti-inflammatory (foot swelling): compared with the model group and the rehmannia group, the swelling inhibition rate of the example 1 group (100:20) was the highest (30.4%, 36.4%, 48.9% respectively, P <0.01), proving that the anti-inflammatory effect is significantly enhanced.
[0027] Analgesia (acetic acid writhing): the analgesic effect of the rehmannia alone is weak. The writhing inhibition rate of the example 1 group (100:20) is as high as 37.0% ( P <0.01), indicating that the processing process systematically adds significant analgesic efficacy to the decoction piece system.
[0028] Arthritis index (AI) and joint swelling degree: in the 49-day observation, the example 1 group (100:20) shows significant and sustained advantages in improving the AI index and joint swelling degree in the later period (35-49 days) (P<0.01), P <0.01), better than single-drug and other ratio groups, reflecting the synergistic treatment value for chronic rheumatic symptoms.
[0029] Conclusion: The new decoction piece prepared in the application not only enhances the anti-inflammatory effect, but also unexpectedly adds analgesic efficacy, and shows synergistic treatment advantages for chronic rheumatic symptoms, perfectly supporting the applications and efficacy declared in claims 4 and 5, see tables 2-5: Table 2 Influence on carrageenan-induced foot swelling
[0030]
[0031] Table 3 Influence on writhing latency and writhing times
[0032]
[0033] Table 4 Effects of prepared rehmannia prepared with fangji juice on joint score index
[0034]
[0035] Table 5 Effects of prepared rehmannia prepared with fangji juice on joint swelling
[0036]
[0037] 2.3, component content determination experiment Taking the prepared slices obtained in example 1 as an example, the characteristic components of rehmannia and fangji are stably detected and quantified in the prepared slices, and the content of the components of rehmannia itself changes significantly, and the detection results show that the content of catalpol in rehmannia is 23.9 mg / g, the content of rehmannia glycoside D is 4.9 mg / g; the content of fangchinoline in fangji is 16.9 mg / g, and the content of sinactine is 11.8 mg / g.
[0038] Conclusion: the prepared slices prepared by the method of the application stably contain and reach the limited four types of characteristic components of rehmannia and fangji and the lower limit of the content, the content of catalpol and rehmannia glycoside D is significantly improved, and the stable introduction of fangji alkaloids is combined, which directly proves from the chemical level that the application successfully creates a new traditional Chinese medicine entity instead of a simple mixture, provides a direct material basis for the new effect of the entity, and see table 6: Table 6 content of characteristic components in each sample
[0039] Note: compared with the rehmannia group, # P <0.05, ## P <0.01; Three, conclusion The preparation method of the decoction piece discloses the specific material basis of the processing process, not only promotes the dissolution and conversion of the effective components (catalpol and rehderin D) of raw rehmannia, but also stably exists the characteristic components (tetrandrine and fangchinoline) of radix stephaniae tetrandrae in the final decoction piece, which is a new entity in chemistry, and the "two-way interaction" and "steady coexistence" of the components are direct evidences of the formation of the new entity by processing, which cannot be achieved by simple water decoction and mixing. The material basis of the decoction piece comprises the characteristic components (catalpol, rehderin D, tetrandrine and fangchinoline) of raw rehmannia and radix stephaniae tetrandrae, and the content of the components of raw rehmannia is significantly changed, which not only enhances the anti-inflammatory effect, but also unexpectedly adds the analgesic effect. P <0.01>.
[0040] In summary, the preparation method of the present application is novel and unique, simple to operate, and successfully prepares the radix stephaniae tetrandrae prepared raw rehmannia decoction piece. The material basis of the decoction piece comprises the characteristic components (catalpol, rehderin D, tetrandrine and fangchinoline) of raw rehmannia and radix stephaniae tetrandrae, and the content of the components of raw rehmannia is significantly changed. In terms of medicinal properties, it realizes the deep integration of "expelling wind and dampness (attacking evil)" and "nourishing yin and blood and calming the heart spirit (upholding the right)", surpasses the simple superposition of the original medicinal materials, becomes a new traditional Chinese medicine raw material with integrated effects, not only enhances the anti-inflammatory effect, but also unexpectedly adds the analgesic effect, and shows a synergistic treatment advantage for chronic rheumatic symptoms, can be effectively used for preparing anti-inflammatory, analgesic, joint swelling improving and antidepressant drugs, and provides technical support for treating rheumatic depression, is a great innovation in the field of traditional Chinese medicine processing, and has practical popularization and application value.
[0041] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. The present application can have various changes and modifications for those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A method for preparing Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae, comprising the following steps: S1, taking Radix Rehmanniae Preparata, cleaning and preparing; S2, taking Radix Stephaniae Tetrandrae, decocting twice with 10 times of water by weight and volume, 30 minutes each time, combining the filtrates, and concentrating to a Radix Stephaniae Tetrandrae decoction with a mass of 0.33-0.67 g / mL; the weight and volume refer to g for solid and mL for liquid; S3, mixing Radix Rehmanniae Preparata with the Radix Stephaniae Tetrandrae decoction at a ratio of 100:10-20 (10:1-2) by mass of raw medicinal materials of Radix Rehmanniae and Radix Stephaniae Tetrandrae, and soaking for 4 hours until the medicine is fully soaked; S4, frying the soaked Radix Rehmanniae Preparata at 70°C until the surface is dark brown and the cross section is black, and drying, to obtain Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae; S5, detection: the detected Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae is a single traditional Chinese medicine entity containing two types of characteristic components of Radix Rehmanniae and Radix Stephaniae Tetrandrae, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehmannioside D is not less than 3.5 mg / g, and both tetrandrine and fangchinoline are contained, which is the finished product.
2. The method for preparing Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae according to claim 1, comprising the following steps: S1, taking 100 g of Radix Rehmanniae Preparata, cleaning and preparing; S2, taking 20 g of Radix Stephaniae Tetrandrae, decocting twice with 10 times of water by weight and volume, 30 minutes each time, combining the filtrates, and concentrating to a Radix Stephaniae Tetrandrae decoction with a mass of 0.67 g / mL (about 30 mL); S3, mixing Radix Rehmanniae Preparata with the Radix Stephaniae Tetrandrae decoction, and soaking for 4 hours until the medicine is fully soaked; S4, frying the soaked Radix Rehmanniae Preparata at 70°C until the surface is dark brown and the cross section is black, and drying, to obtain Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae; S5, detection: the detected Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae is a single traditional Chinese medicine entity containing two types of characteristic components of Radix Rehmanniae and Radix Stephaniae Tetrandrae, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehmannioside D is not less than 3.5 mg / g, and both tetrandrine and fangchinoline are contained, which is the finished product.
3. The method for preparing Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae according to claim 1, comprising the following steps: S1, taking 100 g of Radix Rehmanniae Preparata, cleaning and preparing; S2, taking 10 g of Radix Stephaniae Tetrandrae, decocting twice with 10 times of water by weight and volume, 30 minutes each time, combining the filtrates, and concentrating to a Radix Stephaniae Tetrandrae decoction with a mass of 0.33 g / mL; S3, mixing Radix Rehmanniae Preparata with the Radix Stephaniae Tetrandrae decoction, and soaking for 4 hours until the medicine is fully soaked; S4, frying the soaked Radix Rehmanniae Preparata at 70°C until the surface is dark brown and the cross section is black, and drying, to obtain Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae; S5, detection: the detected Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae is a single traditional Chinese medicine entity containing two types of characteristic components of Radix Rehmanniae and Radix Stephaniae Tetrandrae, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehmannioside D is not less than 3.5 mg / g, and both tetrandrine and fangchinoline are contained, which is the finished product.
4. The method for preparing Radix Rehmanniae Preparata with Radix Stephaniae Tetrandrae according to claim 1, comprising the following steps: S1, taking 100 g of Radix Rehmanniae Preparata, cleaning and preparing; S2, take 15 g of the prepared rhizoma Stephaniae tetrandrae, decoct twice with 10 times the volume of water, each time for 30 minutes, combine the filtrates, and concentrate to a mass of 0.50 g / mL of the prepared rhizoma Stephaniae tetrandrae decoction; S3, mix the prepared rehmannia root with the prepared rhizoma Stephaniae tetrandrae decoction, and soak for 4 hours until the medicine is fully soaked; S4, dry the soaked prepared rehmannia root at 70°C until the surface is dark brown and the cross section is black.
5. S5, detection: the prepared rehmannia root contains two types of characteristic ingredients, namely, the prepared rhizoma Stephaniae tetrandrae and the prepared rehmannia root, wherein the content of catalpol is not less than 20.2 mg / g, the content of rehdermin D is not less than 3.5 mg / g, and the prepared rehmannia root also contains tetrandrine and fangchinoline, which is the finished product.
6. The prepared rehmannia root prepared by the method of any one of claims 1-4 is used for the preparation of a medicine for treating rheumatism, depression, and other diseases.
7. The prepared rehmannia root prepared by the method of any one of claims 1-4 is used for the preparation of an anti-inflammatory, analgesic, and anti-depression medicine.
8. The prepared rehmannia root prepared by the method of any one of claims 1-4 is used in a traditional Chinese medicine composition.