Shampoo containing sodium hyaluronate fermentation filtrate and preparation method thereof

Abstract

The invention discloses shampoo containing sodium hyaluronate fermentation filtrate and a preparation method of the shampoo, and particularly belongs to the field of daily cosmetics. The shampoo contains a composition for relieving scalp and nourishing hair, a surfactant, a cationic component, a hair conditioner, a stabilizer, a thickener, a fat-enriching agent, a preservative, a pH regulator, essence and a solvent, the composition contains a sodium hyaluronate fermentation filtrate, a centella asiatica extract, a liquorice extract, a fructus kochiae extract and a drynaria fortunei rhizome extract, wherein the sodium hyaluronate fermentation filtrate is obtained by carrying out compound fermentation on lactobacillus brevis, lactobacillus acidophilus and lactobacillus gasseri. The shampoo provided by the invention can effectively reduce scalp itching, pricking and tightening, significantly relieve scalp, protect scalp health and maintain scalp micro-ecological balance, scalp sensitivity is effectively repaired, and the shampoo has cleaning power and maintenance functions, and is suitable for people with sensitive scalp and damaged hair quality.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of daily cosmetics, and in particular relates to a shampoo containing sodium hyaluronate fermentation filtrate and a preparation method thereof. BACKGROUND

[0002] In today's hair care market, although there are various types of shampoo products, most shampoos mainly focus on cleaning function and pay little attention to the health care of scalp and hair. Some shampoos contain strong cleaning ingredients such as sulfate, which can effectively remove oil, but excessive cleaning can damage the scalp barrier function, affect the scalp microecological environment, and cause the scalp to dry, itch, and produce more dandruff, and even cause scalp diseases. In addition, some shampoos add natural ingredients such as plant extracts, but lack the synergistic effect of multiple ingredients or have insufficient concentration, making it difficult to simultaneously solve the problems of dry scalp, itching, dandruff, and dry and brittle hair. Some shampoos overemphasize a single function, such as oil control or dandruff removal, while ignoring the nourishment and repair of hair, which cannot meet the needs of consumers for multifunctional shampoo products. Therefore, it has become a technical problem to be solved in the field of hair care to develop a shampoo that can both soothe the scalp and nourish the hair, and has multiple functions such as moisturizing and repairing. SUMMARY

[0003] In view of the deficiencies of the prior art, the present application aims to provide a composition, a shampoo and a preparation method that can effectively soothe the scalp, deeply repair the scalp and nourish the hair.

[0004] To achieve the above-mentioned purpose, the present application discloses the following technical solutions: In a first aspect, the present application provides a composition for soothing the scalp and nourishing the hair, comprising the following components in parts by mass: sodium hyaluronate fermentation filtrate 10-15 parts; gymnema extract 0.1-0.3 parts; licorice extract 2-5 parts; Chinese wolfberry extract 2-5 parts; Rhizoma Drynaria extract 0.5-1 part.

[0005] Preferably, the preparation method of the sodium hyaluronate fermentation filtrate comprises the following steps: Step 1-1. Preparation of fermentation medium: 10% sodium hyaluronate, carbon source, nitrogen source, inorganic salt, surfactant and the rest of water are mixed uniformly and sterilized at 115℃ for 10min for standby, to obtain the fermentation medium; Step 1-2. Compound bacterial solution: mix the activated Lactobacillus brevis bacterial solution, Lactobacillus acidophilus bacterial solution and Lactobacillus gasseri bacterial solution in a volume ratio of 1:1:1 to obtain the compound bacterial solution; Step 1-3. Fermentation culture: inoculate the complex bacteria liquid into the fermentation medium obtained in step 1 at an inoculation amount of 8-10 v / v%, and culture at a rotation speed of 150-200 r / min, 35-37℃, without aeration for 24-36 h, then continue to culture for 12-24 h under an aeration amount of 0.2-0.5 vvm, and then filter the fermentation liquid to remove bacteria to obtain a transparent sodium hyaluronate fermentation filtrate.

[0006] Further preferably, the fermentation medium is composed of 10% sodium hyaluronate, 1% peptone, 1% yeast extract, 0.5% beef extract, 0.05% magnesium sulfate heptahydrate, 0.005% manganese sulfate, 0.2% dipotassium hydrogen phosphate, 0.2% diammonium citrate, 0.1% Tween 80, and the balance of water.

[0007] Further preferably, the viable cell count of the activated Lactobacillus brevis liquid is 1×10 6～8 CFU / mL, the viable cell count of the activated Lactobacillus acidophilus liquid is 1×10 6～8 CFU / mL, and the viable cell count of the activated Lactobacillus gasseri liquid is 1×10 6～8 CFU / mL.

[0008] In a second aspect, the present application provides a soothing and nourishing shampoo, which contains the soothing scalp and hair-nourishing composition of the first aspect.

[0009] Preferably, the addition amount of the soothing scalp and hair-nourishing composition in the shampoo is 3-5 wt%.

[0010] Further preferably, the shampoo further contains a surfactant, a humectant, an emulsion stabilizer, an emulsifier, an antioxidant, a preservative, a pH regulator, a fragrance, and a solvent.

[0011] More preferably, the surfactant is at least one of lauryl polyether surfactants, cocoyl surfactants, sodium olefin sulfonate surfactants, alkyl glucoside surfactants, betaine surfactants, and amino acid surfactants. The pH regulator is at least one of sodium hydroxide, sodium salicylate, sodium citrate, and citric acid. The hair conditioner is polyquaternium-10. The thickening agent is at least one of PEG-160 sorbitan triisostearate, cocamide methyl MEA, PEG-55 propylene glycol oleate, butylene glycol laurate. The stabilizer is trihydroxystearin and / or hydrogenated castor oil.

[0012] In a third aspect, the present application provides a preparation method of the soothing and nourishing shampoo according to the second aspect, comprising the following steps: Step 2-1. Heat a proper amount of deionized water to 70-80℃, and then add the surfactant into the water to dissolve uniformly, to obtain a mixture A for standby; Step 2-2. Mix the hair conditioner and a proper amount of deionized water uniformly to obtain a mixture B; Step 2-3. Homogeneously mix the mixture B with the mixture A, and then sequentially add the pH regulator, the stabilizer, the fatting agent, the thickening agent and the cationic component, and mix uniformly to obtain a mixture C, and keep the mixture C at 20-30 min; Step 2-4. Cool the mixture C to 40-45℃, and then add the soothing and nourishing composition for scalp and hair, the preservative and the fragrance, and mix uniformly, and finally add the pH regulator to adjust the pH value, to obtain the soothing and nourishing shampoo.

[0013] In a fourth aspect, the present application provides an application of the soothing and nourishing composition for scalp and hair according to the first aspect in the preparation of a rinse-off shampoo product with the effects of nourishing, repairing and / or preventing hair breakage.

[0014] In the present application, The sodium hyaluronate fermentation filtrate as an excellent moisturizing agent can absorb and lock a large amount of water, form a hydration film on the surface of the hair, deeply nourish dry and frizzy hair, and improve the smoothness and luster; at the same time, the sodium hyaluronate fermentation filtrate can deeply moisturize the scalp, relieve dryness, tightness, itching and dandruff, strengthen the scalp barrier function, and maintain a healthy and moist scalp environment.

[0015] The centella asiatica extract has the abilities of repairing, anti-inflammatory and antioxidant, can soothe the redness, itching and irritation of the scalp, promote tissue healing, calm sensitive scalp and enhance the barrier function, stimulate collagen synthesis, indirectly strengthen the hair roots, and protect the hair and hair follicles from environmental damage.

[0016] The glycyrrhiza extract has the effects of soothing, anti-inflammatory and anti-allergic, can significantly relieve the itching, redness, allergy and inflammation of the scalp, calm the sensitive and easily irritated scalp, and its antioxidant properties also help to protect the hair follicles, indirectly providing a good growth foundation for the hair.

[0017] The fructus terminaliae chebulae extract has the effects of promoting water metabolism, relieving skin redness and itching, and is often used to improve eczema and urticaria, and has obvious effect of relieving head itching.

[0018] The drynaria rhizome extract can nourish the hair follicles, strengthen the hair roots and hair, reduce the fragile and broken hair, improve the strength, toughness and texture of the hair, promote the microcirculation of the scalp, transport more nutrients to the hair follicles, maintain the vitality of the scalp, and relieve the discomfort of the scalp caused by the weakening of the hair follicle function or the lack of nutrients.

[0019] Advantages of the present application: 1. The present application generates high-activity small molecules such as organic acids, short peptides, etc. through the directional fermentation of hyaluronic acid sodium by the complex flora Lactobacillus brevis, Lactobacillus acidophilus and Lactobacillus gasseri, and simultaneously compounds the plant extracts Centella asiatica extract, licorice extract, Semen Astragali extract and Pteridium aquilinum rhizome extract, significantly improving the inhibition rate of the composition on inflammatory factors and having a significant soothing effect on sensitive scalp; 2. The fermented hyaluronic acid sodium can effectively form a moisturizing film on the surface of the hair, continuously nourishing the hair, and at the same time, the fermented hyaluronic acid sodium contains rich organic acids, which can close the hair scales, reduce hair rubbing damage and water loss, and thus repair the hair and improve the hair toughness and strength; 3. The soothing and nourishing shampoo provided by the present application can effectively reduce the itching, burning, stinging and tightness of the scalp, significantly soothe the scalp, protect the scalp health, maintain the microecological balance of the scalp, and effectively repair the sensitive scalp. The product of the present application has both cleaning and maintenance functions, and is suitable for people with sensitive scalp and damaged hair. DETAILED DESCRIPTION

[0020] The technical solutions of the present application will be described in detail below in combination with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] In order to further illustrate the present application, the following examples are used for detailed description. The raw materials used in the following examples and comparative examples of the present application are all commercially available; the following listed raw material companies are one of the ways to purchase raw materials.

[0022] Lactobacillus brevis: Latin name, purchased from China General Microbiological Culture Collection Center, preservation number CGMCC 1.214; Lactobacillus acidophilus: Latin name, purchased from China General Microbiological Culture Collection Center, preservation number CGMCC 1.3342; Lactobacillus gasseri: Latin name, purchased from China General Microbiological Culture Collection Center, preservation number CGMCC No.1.3224; Centella asiatica extract: Centella asiatica extract; Licorice extract: Licorice (Glycyrrhiza uralensis) extract; Drynaria fortunei rhizome extract: Drynaria fortunei (DRYNARIA FORTUNEI) rhizome extract.

[0023] Preparation of sodium hyaluronate fermentation filtrate Step 1-1. Preparation of fermentation medium: 10% sodium hyaluronate, 1% peptone, 1% yeast extract, 0.5% beef extract, 0.05% magnesium sulfate heptahydrate, 0.005% manganese sulfate, 0.2% dipotassium hydrogen phosphate, 0.2% diamine citrate, 0.1% Tween 80, and the balance water are mixed evenly and then sterilized at 115℃ for 10 minutes to obtain the fermentation medium; Step 1-2. Compound bacterial solution: Mix the activated Lactobacillus brevis, Lactobacillus acidophilus, and Lactobacillus gasseri bacterial solutions in a volume ratio of 1:1:1 to obtain a compound bacterial solution; wherein the viable count of the Lactobacillus brevis solution is 1×10⁻⁶. 8 CFU / mL, viable count of Lactobacillus acidophilus in the culture was 1×10⁻⁶ 8 CFU / mL, viable count of Lactobacillus gasseri in the bacterial culture was 1×10⁻⁶. 8 CFU / mL; Steps 1-3. Fermentation culture: Based on the volume of the culture medium, inoculate the compound bacterial solution into the fermentation culture medium obtained in step 1 at an inoculation rate of 9v / v%. Anaerobically culture at 150 r / min and 37℃ for 24 h, and then continue to culture for 18 h at an aeration rate of 0.2 vvm. After the culture is completed, pass the fermentation broth through a 200-mesh sieve, and then pass it through a 0.22 μm microfiltration for sterilization to obtain sodium hyaluronate fermentation filtrate.

[0024] Preparation of a composition for soothing the scalp and nourishing the hair After accurately weighing and mixing the raw materials according to the mass ratio in Table 1, the composition is obtained.

[0025] Table 1. Mass ratio of each component in the composition

[0026] Performance testing 1. Anti-inflammatory performance test 1.1 Main Reagents Mouse mononuclear macrophages (RAW264.7), DMEM high glucose medium, fetal bovine serum, IL-1β ELISA kit, LPS: lipopolysaccharide.

[0027] 1.2 Sample to be tested Test sample group: Take compositions 1-9 respectively, dilute with cell culture solution to concentrations of 0.15wt%, 0.5wt%, 1wt%, 1.25wt%, 1.5wt%, and 2wt%, and then perform cytotoxicity test (MTT method) to determine the safe working concentration. Test verifies that when the concentration is ≤1.5wt%, the corresponding cell activity of compositions 1-9 is ≥90%, and the following test is performed at a concentration of 1wt%.

[0028] 1.3 Test method 1.3.1 Cell activation After the cryopreserved mouse monocyte macrophages (RAW264.7) were recovered, they were cultured in a T25 culture flask with 5mL complete culture medium (DMEM high-sugar culture medium containing 10% fetal bovine serum) and placed in a 37℃, 5v / v% CO2 incubator for 24h, then the liquid was changed and subcultured for use.

[0029] 1.3.2 Grouping and dosing RAW 264.7 cells cultured to the logarithmic growth phase were inoculated in a 96-well plate at a density of 1×10 5 cells / well, and were grouped: normal group, model control group, test sample group, with 6 holes repeated as parallel for each group. Except for the normal group cells, the cells in the remaining groups were added with complete culture medium containing a final concentration of 1µg / mL LPS, and after 24h of culture, the samples were added according to Table 2 and cultured for another 24h. Table 2 Stimulation and dosing

[0030] After 24h of culture, the cell supernatant was collected, and the content of IL-1β in the cell supernatant was determined according to the operation steps of the ELISA kit.

[0031] 1.4 Data statistics Statistical analysis was performed using SPSS19.0 statistical software package. If the test data is normally distributed, independent T test method is used for statistical analysis; if the test data is not normally distributed, rank sum test method is used for statistical analysis. The statistical method uses double-tailed test, and the test level a=0.05.

[0032] 1.5 Test results ; In the formula: A0 is the average value of the IL-1β content of the normal group; A1 is the average value of the IL-1β content of each test sample group; A2 is the average value of the IL-1β content of the model control group.

[0033] Table 3 IL-1β content and inhibition rate of each group

[0034] Note: "a" means significant difference compared with the model control group, P<0.05, "aa" P<0.01, "aaa" P<0.001; "b" means significant difference compared with sample group 3, P<0.05, "bb" P<0.01, "bbb" P<0.001.

[0035] 1.6 Result analysis According to the results in Table 3, the inhibition rates of sample groups 1, 2, 3 and 4 on inflammatory factors were 76.11%, 73.66%, 78.42% and 76.90% respectively, among which sample group 3 had the best effect and was significantly different from the model control group, which indicated that the sodium hyaluronate fermentation filtrate, centella asiatica extract, glycyrrhiza extract, kochia sieboldiana extract and drynaria rhizome extract provided by the application had good inhibitory effect on inflammatory factors when used in combination, and effectively reduced the occurrence of inflammation. Sample group 4 was sodium hyaluronate solution without complex bacterial liquid fermentation treatment, and the anti-inflammatory performance was significantly reduced, which was significantly different from sample group 3, indicating that the fermentation filtrate after complex bacterial liquid fermentation synthesized a large amount of anti-inflammatory active ingredients, and improved the anti-inflammatory and soothing effect of the overall formula. Sample groups 6-9 respectively lacked centella asiatica extract, glycyrrhiza extract, kochia sieboldiana extract and drynaria rhizome extract, which led to a significant decrease in the inhibition rate of the composition on inflammatory factors (P<0.05), which proved that the sodium hyaluronate fermentation filtrate, centella asiatica extract, glycyrrhiza extract, kochia sieboldiana extract and drynaria rhizome extract in the composition played a key role in anti-inflammatory soothing.

[0036] 2 In vitro hair strand tensile strength influence test 2.1 Test equipment Instron3400 electronic universal material testing machine 2.2 Hair strand selection A certain amount of flat black healthy in vitro hair strands were obtained, and randomly divided into 10 groups, a control group and sample groups 1-9, 1 strand per group, and 2.5g per strand.

[0037] 2.3 Test sample Sample groups 1-9 were respectively taken, mixed with deionized water at a mass ratio of 1:9, and uniformly dispersed at 5000r / min to obtain test samples 1-9.

[0038] 2.3 Hair strand pretreatment Before testing, all samples were cleaned with 1 mL of 10% sodium dodecyl sulfate, twice, and then dried and combed 10 times with a narrow-tooth comb. After pretreatment, the hair strands were hung in a constant temperature and humidity chamber at 20 ± 1 °C and RH 50 ± 5% for drying and equilibration for 24 h.

[0039] 2.4 Hair strand treatment method Test sample group: 0.1 g of the test sample per gram of hair was applied to the corresponding group of hair strands, and rubbed for 30 s. The hair strands were then washed with water at 37 °C and a flow rate of 4 L / min for 30 s, and combed every 5 s during washing. The treatment was repeated twice, and the hair strands were finally dried and combed 10 times with a narrow-tooth comb.

[0040] Control group: The hair strands in the control group were treated with deionized water instead of the test sample, according to the treatment method for the test sample group.

[0041] After treatment, the hair strands were hung in a constant temperature and humidity chamber at 20 ± 1 °C and RH 50 ± 5% for overnight equilibration, and treated once a day for 10 consecutive days.

[0042] 2.5 Test method Ten hair strands were randomly selected from each group, and an Instron 3400 electronic universal material testing machine was used to stretch the hair strands at a stretching speed of 15 mm / min until the maximum tensile force that the hair strands could withstand when broken, which was recorded as the breaking strength (N). The average value was then calculated.

[0043] 2.6 Test results The increase in breaking strength was calculated according to the following formula: ; In the formula, N1 represents the average value of the breaking strength of the test sample group. N0 represents the average value of the breaking strength of the control group.

[0044] Table 4 Performance test results Group Breaking strength / N Growth rate / % Control group 4.39 / Test sample group 1 (composition 1) 6.32 43.96% Test sample group 2 (composition 2) 6.28 43.05% Test sample group 3 (composition 3) 6.17 40.55% Test sample group 4 (composition 4) 6.23 41.91% Test sample group 5 (composition 5) 5.05 15.03% Test sample group 6 (composition 6) 6.20 41.23% Test sample group 7 (composition 7) 6.93 57.86% Test sample group 8 (composition 8) 6.91 57.40% Test sample group 9 (composition 9) 6.57 49.66%

[0045] 2.7 Result analysis According to the results of Table 4, the breaking strength of the test sample groups 1-4 and 6-9 showed a significant increase compared with the control group, among which the breaking strength growth rates of test sample groups 7 and 8 reached 57.86% and 57.40% respectively, because the test sample groups 1-4 and 6-9 all contained sodium hyaluronate fermentation filtrate, which strengthened the toughness of the hair. Although the breaking strength of the test sample group 5 also increased compared with the control group, the effect was not as good as that of the other groups, indicating that the fermented sodium hyaluronate could effectively form a moisturizing film on the surface of the hair, continuously nourishing the hair. At the same time, the fermented sodium hyaluronate contained rich organic acids, which could close the hair scales, reduce hair friction damage and water loss, and thus repair the hair and improve the toughness and strength of the hair.

[0046] Preparation of soothing and nourishing shampoo The raw materials were accurately weighed according to the mass percentage in Table 5; Step 2-1. Heat an appropriate amount of deionized water to 80℃, add the surfactant into it and dissolve uniformly to obtain mixture A for standby; Step 2-2. Mix the hair conditioner and an appropriate amount of deionized water uniformly to obtain mixture B; Step 2-3. Homogeneously mix mixture B with mixture A at 80℃, then sequentially add the pH adjuster, stabilizer, fat-riching agent, thickening agent and cationic component, mix uniformly to obtain mixture C, and keep warm for 20 min; Step 2-4. Cool mixture C to 40℃, add the soothing scalp and nourishing hair composition, preservative, fragrance and mix uniformly, finally add the pH adjuster to adjust the pH value to obtain the soothing and nourishing shampoo.

[0047] Table 5 Mass percentage of shampoo raw materials

[0048] Efficacy verification 1 Test sample The shampoo prepared in Example 3 was applied.

[0049] 2 Test method 2.1 Subjects The subjects were 30 people, including 5 men and 25 women, aged 35-45 years old.

[0050] 2.2 Inclusion criteria (1) Sensitive scalp; (2) No allergic diseases, no history of cosmetic and other external preparation allergies; (3) Voluntarily participate in the test and sign the written informed consent form.

[0051] 2.3 Exclusion criteria (1) Pregnant or lactating women, or those with recent plans for pregnancy; (2) Those with bacterial or viral infection in the head; (3) Those with eczema, psoriasis, tinea capitis, pityriasis lichenoides or obvious seborrheic dermatitis in the head; (4) Those who have used hormone products on the test site or taken hormones in the past 4 weeks; (5) Those who have applied any anti-inflammatory drugs to the test site in the past 2 months; (6) Those with highly sensitive constitution; (7) Those who are judged by the trial director to be unsuitable as subjects of this trial.

[0052] 2.4 Test Environment The subjects should not use any cosmetics on their heads on the day of the test; they should sit in a constant temperature and humidity environment (temperature 20-22°C, relative humidity 40%RH-60%RH) for more than 20 minutes before the test, during which they should not wear hats or other head coverings.

[0053] 2.5 Test Method There are a total of 3 data collection sessions during the trial, at the beginning of the use of the test sample (week 0), 2 weeks after the use of the test sample and 4 weeks after the use of the test sample.

[0054] The trial uses a combination of visual assessment and self-assessment by the subjects, and the scores are each 50%.

[0055] Visual Assessment: At each visit, the subjects' entire head is divided into 3 zones, and a skin mirror is used to take a picture of a fixed location in each zone, and a dermatologist scores the degree of redness of the scalp in the picture, with the scores being graded according to 0 = normal, 1 = slight pink, 2 = pink, 3 = deep pink, and the average of the scores of the 3 locations is the visual assessment score of the dermatologist.

[0056] Self-Assessment: The subjects score the itching, burning, stinging and tightness of the scalp (each indicator weighs 25%), with the scores being graded according to 0 = none, 1 = mild, 2 = moderate, 3 = severe, and the self-assessment score is obtained by multiplying the sum of the scores of each indicator by 25%.

[0057] 2.6 Data Statistics The rank sum test is applied to the two related samples using SPSS software, and P<0.05 is statistically significant.

[0058] 2.7 Trial Results Table 6 Statistical Results of Scalp Scores

[0059] 2.8 Analysis of Results According to the result, the soothing and nourishing shampoo can effectively reduce the itching, burning, stinging and tightness of the scalp, significantly soothe the scalp, protect the scalp health, maintain the microecological balance of the scalp, and effectively repair the scalp sensitivity.

[0060] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the foregoing detailed description of the present application is made with reference to the foregoing embodiments, for those skilled in the art, it still can be modified, or the equivalent replacement of part of the technical features recorded in the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A soothing scalp, nourishing hair composition characterized in that, The composition comprises the following components by mass fraction: Sodium hyaluronate fermentation filtrate 10-15 parts; Centella asiatica extract 0.1-0.3 parts; Glycyrrhiza extract 2-5 parts; Fructus terminaliae extract 2-5 parts; Rhizoma cyatheae extract 0.5-1 part.

2. The composition of claim 1, wherein, The preparation method of the sodium hyaluronate fermentation filtrate comprises the following steps: Step 1-1. Preparation of fermentation medium: 10% sodium hyaluronate, carbon source, nitrogen source, inorganic salt, surfactant and the rest of water are mixed uniformly, sterilized at 115℃ for 10 min and prepared for use, to obtain the fermentation medium; Step 1-2. Compound bacteria solution: the activated Lactobacillus brevis, Lactobacillus acidophilus and Lactobacillus gasseri are mixed at a volume ratio of 1:1:1 to obtain the compound bacteria solution; Step 1-3. Fermentation culture: the compound bacteria solution is inoculated into the fermentation medium obtained in step 1 at an inoculation amount of 8-10 v / v%, and cultured at a rotation speed of 150-200 r / min, 35-37℃, without aeration for 24-36 h, and then cultured for 12-24 h under an aeration amount of 0.2-0.5 vvm, and then the fermentation liquid is filtered to remove bacteria to obtain the sodium hyaluronate fermentation filtrate.

3. The composition of claim 2, wherein, The fermentation medium is composed of 10% sodium hyaluronate, 1% peptone, 1% yeast extract, 0.5% beef extract, 0.05% magnesium sulfate heptahydrate, 0.005% manganese sulfate, 0.2% dipotassium hydrogen phosphate, 0.2% diammonium citrate, 0.1% Tween 80 and the rest of water.

4. The composition of claim 2, wherein, The viable cell count of the activated Lactobacillus brevis bacterial solution is 1 x 10 6～8 CFU / mL, the viable cell count of the activated Lactobacillus acidophilus bacterial solution is 1 x 10 6～8 CFU / mL, and the viable cell count of the activated Lactobacillus gasseri bacterial solution is 1 x 10 6～8 CFU / mL.

5. A soothing and nourishing shampoo, characterized in that, The shampoo contains the soothing scalp and hair-nourishing composition according to any one of claims 1-4.

6. The soothing and nourishing shampoo according to claim 5, wherein, The addition amount of the soothing scalp and hair-nourishing composition in the shampoo is 3-5 wt%.

7. The soothing, nourishing shampoo according to claim 5 or 6, characterized in that It also contains surfactants, cationic components, hair conditioners, stabilizers, thickening agents, rich fat agents, preservatives, pH regulators, fragrances and solvents.

8. The soothing, nourishing shampoo of claim 7, wherein, The surfactant is at least one of lauryl polyether surfactant, cocoyl surfactant, sodium olefin sulfonate surfactant, alkyl glucoside surfactant, betaine surfactant and amino acid surfactant. The pH regulator is at least one of sodium hydroxide, sodium salicylate, sodium citrate and citric acid.

9. A method of preparing the soothing and nourishing shampoo according to any one of claims 5 to 8, characterized in that, The following steps are included: Step 2-1. Heat an appropriate amount of deionized water to 70-80℃, add the surfactant and dissolve uniformly to obtain mixture A for standby; Step 2-2. Mix the hair conditioner and an appropriate amount of deionized water uniformly to obtain mixture B; Step 2-3. Homogeneously mix mixture B with mixture A, then add the pH regulator, stabilizer, rich fat agent, thickening agent and cationic component in sequence, mix uniformly to obtain mixture C, and keep warm for 20-30 min; Step 2-4. Cool mixture C to 40-45℃, add the soothing scalp and hair-nourishing composition, preservative, fragrance and mix uniformly, and finally add the pH regulator to adjust the pH value to obtain the soothing and nourishing shampoo.

10. Use of a soothing scalp, nourishing hair composition according to any one of claims 1 to 4, for the manufacture of a rinse-off shampoo product having a nourishing, repairing and / or anti-breakage effect.

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