Skin repair composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and discloses a skin repair composition as well as a preparation method and application thereof. The skin repair composition is prepared from the following components in percentage by mass: 0.5 to 5 percent of herba centellae extract, 1 to 8 percent of ectoine, 1 to 3 percent of alpha-bisabolol, 0.1 to 0.5 percent of guaiacol, 0.03 to 1 percent of rhizoma curcumae longae extract, 0.2 to 0.5 percent of tetrahydrocurcumin, 0.1 to 1 percent of glycyrrhetinic acid, 0.01 to 0.2 percent of carbenoxolone sodium, 0.1 to 3 percent of ceramide, 10 to 20 percent of phospholipid, 3 to 8 percent of structured lipid MCM, 2 to 6 percent of surfactant, 15 to 30 percent of cosurfactant, 0.05 to 0.15 percent of soluble zinc salt, 0.01 to 0.1 percent of recombinant phospholipase A2 and the balance of ultrapure water. The skin repair composition disclosed by the invention can act on three levels of skin cells, epidermis and dermis, can play the effects of resisting inflammation, resisting oxidation and enhancing immunity through a multi-target action mechanism, and meanwhile, establishes a dual repair system of endogenous healing activation and external barrier reinforcement from internal and external perspectives, so that skin problems are comprehensively reduced, and the skin repair effect is improved. The skin health is obviously improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the cosmetic technology field, in particular to a skin repair composition and a preparation method and application thereof. BACKGROUND

[0002] In daily life, ultraviolet rays, environmental pollution and frequent invasion of external allergens can easily cause skin inflammation, allergic reactions and accelerate skin aging. At the same time, unhealthy habits such as staying up late, greasy diet and improper skin care also affect skin health. Globally, about 71% of people believe that their skin is less resistant, and after being stimulated by external factors, they are prone to symptoms such as stinging, burning, itching and erythema.

[0003] By repairing the skin with skin care products, the skin can return to a healthy state, greatly improving the quality of life and health status. Although there are many plant extract skin care products on the market, they have the following problems: on the one hand, the efficacy is single and it is difficult to repair various skin problems; on the other hand, some active ingredients of plant extracts are easily destroyed by light, oxygen and temperature, and have poor stability; they are difficult to disperse uniformly, affecting the compatibility of the formula. In addition, some active ingredients of plant extracts have large molecular weight and are difficult to penetrate the skin lipid barrier, and the transdermal effect is not ideal. The above leads to the existing plant extract skin care products having single efficacy and poor skin repair effect.

[0004] Therefore, it is urgent to develop a mild, safe and non-irritating skin repair composition with good skin repair effect. SUMMARY

[0005] The present application provides a skin repair composition and a preparation method and application thereof, which aims to solve the technical problems of single efficacy and poor skin repair effect of existing plant extract cosmetics.

[0006] In order to achieve the above purpose, the following technical solutions are adopted in the present application.

[0007] In a first aspect of the present application, a skin repair composition is provided, which comprises the following components in mass percentage:

[0008] Centella asiatica extract 0.5%~5%, ectoine 1%~8%, α-bisabolol 1%~3%, guaiac oil 0.1%~0.5%, turmeric extract 0.03%~1%, tetrahydrocurcumin 0.2%~0.5%, glycyrrhetinic acid 0.1%~1%, sodium glycinate 0.01%~0.2%, ceramide 0.1%~3%, phospholipids 10%~20%, structured lipid MCM 3%~8%, surfactant 2%~6%, cosurfactant 15%~30%, soluble zinc salt 0.05%~0.15%, recombinant phospholipase A2 0.01%~0.1%, balance ultrapure water.

[0009] Preferably, the structured lipid MCM is a mannose-conjugated medium-chain triglyceride derivative.

[0010] More preferably, the mannose conjugated medium-chain triglyceride derivative has a triglyceride core backbone, an ω-3 polyunsaturated fatty acid chain, and a terminal mannose group; the ω-3 polyunsaturated fatty acid chain is eicosapentaenoic acid.

[0011] Preferably, the soluble zinc salt is zinc lactate.

[0012] Preferably, the phospholipid includes at least one of soybean lecithin, cephalin, sphingomyelin, hydrogenated lecithin, or egg yolk lecithin.

[0013] Preferably, the surfactant is at least one of Tween 20, castor oil polyoxyethylene ether 40, Span 80, sorbitan monooleate, polyglycerol-10 laurate, polyglycerol-10 oleate, or polyoxyethylene hydrogenated castor oil.

[0014] The surfactant is at least one of Tween 80, castor oil polyoxyethylene ether 40, Span 80, sorbitan monooleate, polyglycerol-10 laurate, polyglycerol-3 polyricinoleate, or polyoxyethylene hydrogenated castor oil.

[0015] The co-surfactant is at least one of PEG400, 1,2-propylene glycol, glycerin, 1,3-butanediol, polypropylene glycol, ethoxydiethylene glycol, or glycerol polyether-26.

[0016] A second aspect of this application provides a method for preparing the above-mentioned skin repair composition, comprising:

[0017] S1, α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM and phospholipids are mixed and stirred until melted under an inert atmosphere to obtain the oil phase;

[0018] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate, and soluble zinc salt were added to water and ultrasonically dispersed uniformly under an inert atmosphere to obtain an aqueous phase;

[0019] S3, mix the surfactant and co-surfactant evenly, then add them to the aqueous phase and disperse them by ultrasonication to obtain a mixture;

[0020] S4. Under the magnetic stirrer, the mixture is slowly added to the oil phase, then recombinant phospholipase A2 is added, and after stirring, homogenization and shearing are performed to obtain the colostrum.

[0021] S5, the colostrum is subjected to a first homogenization process, the product is cooled to 25°C, and then subjected to a second homogenization process to obtain a skin repair composition.

[0022] Preferably, stirring is carried out in S1 at 45-55℃;

[0023] In S2, the ultrasonic dispersion time is 20~40 min, and the ultrasonic frequency is 40KHz;

[0024] In S3, the ultrasonic dispersion time is 10~20 min, and the ultrasonic frequency is 40KHz;

[0025] In S4, the mixture is slowly added to the oil phase at a dropping rate of 1-2 drops per second; the stirring rate is 1000 r / min and the stirring time is 20-40 min; the shearing speed is 8000-12000 rpm and the shearing time is 5-10 min.

[0026] In S5, the homogenization process is carried out at a pressure of 30 MPa for 7 to 12 minutes.

[0027] A third aspect of this application provides the use of the above-described skin repair composition in products for skin repair.

[0028] A fourth aspect of this application provides a cosmetic for skin repair, comprising 0.1 to 10 wt% of the above-described skin repair composition.

[0029] Compared with the prior art, the beneficial effects of this application are as follows:

[0030] The skin repair composition of this application can act at the three levels of skin cells, epidermis and dermis, and exert anti-inflammatory, antioxidant and immune-enhancing effects through a multi-target mechanism. At the same time, it establishes a dual repair system from both internal and external perspectives, which activates internal healing power and strengthens the external protective barrier, comprehensively reduces skin problems and significantly improves skin health.

[0031] At the cellular level, Centella asiatica extract and ceramides promote cell proliferation and accelerate cell renewal. Ectoin prevents stress factors from damaging cells, increases cellular stress resistance, and accelerates cell repair. In the epidermis, α-bisabolol, glycyrrhetinic acid, and sodium glycinate eliminate micro-inflammation in the skin, alleviating acne, rashes, and other skin problems related to frequent skin irritation. Turmeric extract, guaiac oil, Centella asiatica extract, and tetrahydrocurcumin exert antioxidant activity, protecting keratinocytes and fibroblasts from oxidative stress damage. In the dermis, turmeric extract inhibits collagen damage, and Centella asiatica extract promotes collagen synthesis, jointly enhancing skin elasticity and reducing wrinkles.

[0032] From the perspective of endogenous healing activation, guaiac oil inhibits mast cell degranulation, suppresses allergic reactions, stimulates the lymphatic system to fight infection, and enhances the immune system; glycyrrhetinic acid inhibits the production and release of immunoglobulin E (IgE) and prostaglandins, regulates immune function, and relieves the toxic side effects of external factors on the skin; ectoine enhances skin immunity; centella asiatica extract promotes barrier damage repair; and ceramides assist in thickening of the stratum corneum and repair of damaged skin.

[0033] From the perspective of strengthening the skin's external protective barrier, Centella asiatica extract, turmeric extract, tetrahydrocurcumin, glycyrrhetinic acid, and sodium glycinate can improve and restore the changes in the appearance and cell structure of skin fibroblasts induced by ultraviolet radiation or external stimuli. Alpha-bisabolol acts on skin exposed to ultraviolet radiation, exerting a calming and soothing effect and reducing sunburn redness. Ceramides and ectoine enhance skin hydration, forming a hydration layer to protect the skin and strengthen the skin barrier function.

[0034] To address the issue of ceramide's high crystallinity and difficulty in compatibility with essential oils, this application utilizes tetrahydrocurcumin as a "molecular bridge," with the hydrophobic end binding to the alkyl chain of ceramide and the hydrophilic end complexing with the polar group of guaiac oil, forming a stable complex. This complex maintains stability through hydrophobic interactions, hydrogen bonds, and van der Waals forces, reducing the system's free energy and preventing ceramide crystallization or essential oil stratification.

[0035] Furthermore, the skin composition prepared in this application is a nanoemulsion, which not only has high stability and does not separate into layers during autoclaving or centrifugation, but also has the characteristics of low viscosity, small and uniform particle size, which helps to disrupt the tight structure of the stratum corneum, allowing active ingredients to enter the stratum corneum smoothly, significantly improving the transdermal absorption effect, and having a certain sustained-release and targeted effect; it is both hydrophilic and lipophilic, has a high solubility for active ingredients, and also has certain antibacterial, moisturizing and repairing effects on the skin. Attached Figure Description

[0036] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments recorded in this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0037] Figure 1 The graph shows the TNF-α content detection results of the samples in the examples and comparative examples during the anti-inflammatory capacity test;

[0038] Figure 2 The graph shows the detection results of IL-6 content in the anti-inflammatory capacity test of the samples in the examples and comparative examples;

[0039] Figure 3 The image shows the cell viability test results of HaCaT cells in the protection experiment against H2O2-induced oxidative damage, for the samples in the examples and comparative examples.

[0040] Figure 4 The image shows the skin stratum corneum moisture content test results of the samples in the examples and comparative examples during human efficacy testing;

[0041] Figure 5 The image shows the skin elasticity test results of the samples in the human efficacy test for the examples and comparative examples. Detailed Implementation

[0042] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0043] In the following description of this embodiment, the terms "including", "comprising", "having", and "containing" are all open-ended terms, meaning that they include but are not limited to.

[0044] In the following description of this embodiment, the term "and / or" is used to describe the association relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, B existing alone, and A and B existing simultaneously. A and B can be singular or plural. The character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0045] In the following description of this embodiment, the term "at least one" means one or more, and "more than one" means two or more. "At least one of the following" or similar expressions refer to any combination of these items, including any combination of single or multiple items. For example, "at least one of a, b, or c", or "at least one of a, b, and c", can both mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple.

[0046] The terminology used in the embodiments of this application is for the purpose of describing particular embodiments only and is not intended to be limiting of this application. The singular forms "a" and "the" as used in the embodiments of this application and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise.

[0047] Those skilled in the art should understand that, in the following description of the embodiments of this application, the sequence of numbers does not imply the order of execution. Some or all steps may be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0048] Those skilled in the art will understand that the numerical ranges in the embodiments of this application should be understood as each intermediate value between the upper and lower limits of the specifically disclosed range. Each smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this application. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0049] Unless otherwise stated, the technical / scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. While this application describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this application. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0050] This application leverages the advantages of nanoemulsions, combining a dual repair system that activates the healing power of the repair composition from within and strengthens the external protective barrier, with the targeting properties of structured lipid MCMs, to develop an all-around repair nanoemulsion with good transdermal absorption, high skin retention, strong targeting, convenient and safe use, capable of solving various skin problems, and suitable for various skin types and usage scenarios.

[0051] In a first aspect, this application provides a skin repair composition comprising, by weight percentage, the following components:

[0052] Centella asiatica extract 0.5%~5%, ectoine 1%~8%, α-bisabolol 1%~3%, guaiac oil 0.1%~0.5%, turmeric extract 0.03%~1%, tetrahydrocurcumin 0.2%~0.5%, glycyrrhetinic acid 0.1%~1%, sodium glycinate 0.01%~0.2%, ceramide 0.1%~3%, phospholipids 10%~20%, structured lipid MCM 3%~8%, surfactant 2%~6%, cosurfactant 15%~30%, soluble zinc salt 0.05%~0.15%, recombinant phospholipase A2 0.01%~0.1%, balance ultrapure water.

[0053] In the skin repair composition of this application, the functions of the plant ingredients are as follows:

[0054] Centella asiatica is renowned for its remarkable restorative abilities, demonstrating significant efficacy in enhancing wound healing, restoring the nervous system and brain cells, and treating psoriasis, lupus, and ulcers. The abundant pentacyclic triterpenoid saponins, secondary metabolites accumulated during its growth, are crucial to its pharmacological effects. These include asiaticoside, hydroxyasiaticoside, asiaticoside, and asiaticoside acid, all of which exhibit significant biological effects in promoting skin damage repair. They also possess anti-inflammatory, antibacterial, antioxidant, and collagen-synthesizing properties. Using Centella asiatica extract for skincare can enhance skin hydration, protect the damaged skin barrier, reduce allergies, and relieve skin pain and itching; inhibit collagen breakdown and delay skin aging; and improve and restore UV-induced changes in the appearance and structure of skin fibroblasts.

[0055] Ectoin has a wide range of cell protection functions. It can strongly complex water molecules to form organized water molecule clusters, thereby increasing the fluidity of lipid head groups in biomembranes, improving the ability of biomembranes to withstand extreme environments such as temperature or osmotic pressure, and further accelerating cell repair. It also effectively protects Langerhans cells (skin's immune cells, located in the epidermis), enhances skin immunity, and resists damage from external stimuli such as ultraviolet radiation.

[0056] Alpha-bisabolol is a classic soothing and redness-reducing active ingredient, key to the anti-inflammatory effects of plants such as chamomile and chamomile. Alpha-bisabolol effectively reduces the production of peroxidase (a major substance causing inflammation in the body), inhibits NLRP3 inflammasome activity and the TLR4 / NF-κB / MAPK signaling pathway, and has a good inhibitory effect on both acute and chronic inflammation. Using alpha-bisabolol for skin care can effectively reduce acne, rashes, rosacea, psoriasis, and skin problems associated with frequent skin irritation; when applied to skin exposed to ultraviolet radiation, it has a calming and soothing effect, reducing sunburn and redness; it also helps other beneficial ingredients penetrate deeper into the skin.

[0057] The main chemical components of guaiac oil are guaiacol, rutinol, β-cineole, and α-rutinene, which can accelerate the healing process of skin wounds, reduce swelling and pain, have anti-inflammatory and antibacterial effects, provide antioxidant and anti-aging benefits, and inhibit allergies. Guaiac oil suppresses allergic reactions by inhibiting mast cell degranulation, and fights infection and enhances the immune system by scavenging free radicals and stimulating the lymphatic system. In skin care, it can improve skin texture, strengthen the skin barrier, and delay aging.

[0058] The main bioactive components of turmeric extract are curcuminoids and volatile oils, which possess strong antioxidant and anti-aging effects. They can also significantly improve rough skin and enhance skin immunity. Curcumin, in particular, effectively scavenge reactive oxygen species and resist lipid peroxidation, preventing DNA breakage, enzyme inactivation, and damage to collagen, thus protecting keratinocytes and fibroblasts from external factors such as ultraviolet (UV) radiation and environmental pollution. Tetrahydrocurcumin, a major reducing metabolite of curcumin, exhibits better chemical stability, water solubility, and lipid solubility than curcumin, and its antioxidant activity is more than three times higher than that of curcumin, remaining stable within a pH range of 3-9.

[0059] Glycyrrhetinic acid can bind to corticosteroid receptors, exhibiting mild mineralocorticoid-like effects and unique efficacy in anti-inflammation and anti-allergy. It can inhibit the production and release of immunoglobulin E (IgE) and prostaglandins, regulate skin's immune function, and relieve the toxic side effects of external factors on the skin. It can also inhibit the production and release of inflammatory mediators, eliminate micro-inflammation on the skin surface, prevent pigmentation spots caused by inflammation, and quickly brighten skin tone. Glycyrrhetinic acid is also an antioxidant, which can resist photodamage and photoaging caused by sunlight by inhibiting the production of reactive oxygen species and preventing the formation of oxidized DNA fragments. Sodium glycyrrhetinate, the disodium salt of glycyrrhetinic acid succinate, is a derivative of glycyrrhetinic acid and has good anti-inflammatory and anti-inflammatory effects. Sodium glycyrrhetinate can enhance the skin barrier function, improve immune function, and accelerate tissue regeneration. Its anti-inflammatory effect is superior to that of glycyrrhetinic acid, and it has fewer skin side effects.

[0060] Ceramides are an important component of the lipids in the stratum corneum of the skin, playing a crucial role in maintaining the homeostasis of the skin barrier function. Ceramides can form a tightly stacked network lipid structure through intermolecular non-covalent bonds, locking in skin moisture; they can significantly upregulate the transcription and protein expression levels of filaggrin mRNA, thereby promoting the synthesis and accumulation of natural moisturizing factors such as polycarboxylic acids; ω-OH-containing ceramides can covalently bind to the inner lining protein of the keratinized capsule of keratinocytes, thereby connecting the lipid matrix and keratinocytes, enhancing the skin barrier function, and reducing the penetration of external irritants into the skin. Therefore, ceramides can assist in thickening the stratum corneum, improve skin tolerance, and have moisturizing, anti-allergic, and repairing effects.

[0061] In this application, the structured lipid MCM is a mannose-conjugated medium-chain triglyceride derivative, which has a triglyceride core backbone, an ω-3 polyunsaturated fatty acid chain, and terminal mannose modification, forming a specific structure of "mannose-medium-chain fatty acid-glycerol-ω-3 fatty acid"; wherein the ω-3 polyunsaturated fatty acid chain is eicosapentaenoic acid. It can specifically target skin lymphatic vessel M cells through "mannose receptor-mediated endocytosis," releasing ω-3 fatty acids to inhibit the synthesis of pro-inflammatory factors, while the mannose regulates the antigen presentation function of M cells, reducing excessive immune responses and improving skin health.

[0062] In this application, a soluble zinc salt acts as a metal ion ligand. By forming a coordination bond with the carboxyl group of glycyrrhizic acid, glycyrrhetinic acid is embedded in the hydrophobic cavity, thereby improving the imbalance between the water solubility and lipid solubility of glycyrrhetinic acid and sodium glycyrrhizate, and optimizing the overall lipid-water partition coefficient. Simultaneously, the addition of zinc ions enhances transdermal absorption. The preferred soluble zinc salt is zinc lactate.

[0063] In this application, recombinant phospholipase A2 (rPLA2) utilizes the specific hydrolytic action of the enzyme on phospholipids to induce structural rearrangement of lipid components in the system, forming nanoscale ordered aggregates and triggering self-assembly, resulting in smaller particle size and more uniform distribution. The active ingredients in the system can embed into the hydrophobic / hydrophilic regions of the self-assembled structure, stabilizing the nanocarrier and regulating release behavior. The phospholipids are selected from at least one of soybean lecithin, cephalin, sphingomyelin, hydrogenated lecithin, or egg yolk lecithin.

[0064] In this application, the surfactant is at least one of Tween 20, castor oil polyoxyethylene ether 40, Span 80, sorbitan monooleate, polyglycerol-10 laurate, polyglycerol-10 oleate, or polyoxyethylene hydrogenated castor oil; the co-surfactant is at least one of PEG400, 1,2-propylene glycol, glycerin, 1,3-butanediol, polypropylene glycol, ethoxydiethylene glycol, or glycerol polyether-26.

[0065] The skin repair composition of this application can work at the three levels of skin cells, epidermis and dermis through the synergistic effect of each component. It exerts anti-inflammatory, antioxidant and immune-enhancing effects through a multi-target mechanism. At the same time, it establishes a dual repair system from both internal and external perspectives, which activates internal healing power and strengthens the external skin barrier, comprehensively reduces skin problems and significantly improves skin health.

[0066] Specifically, at the cellular level, Centella asiatica extract and ceramides promote cell proliferation and accelerate cell renewal; ectoine prevents stress factors from damaging cells, increases cell stress resistance, and accelerates cell repair. In the epidermis, α-bisabolol, glycyrrhetinic acid, and sodium glycerate eliminate micro-inflammation, alleviating acne, rashes, and other skin problems related to frequent skin irritation; turmeric extract, guaiac oil, Centella asiatica extract, and tetrahydrocurcumin exert antioxidant activity, protecting keratinocytes and fibroblasts from oxidative stress damage. In the dermis, turmeric extract inhibits collagen damage, and Centella asiatica extract promotes collagen synthesis, jointly enhancing skin elasticity and reducing wrinkles.

[0067] In terms of endogenous healing activation, guaiac oil inhibits mast cell degranulation, suppresses allergic reactions, stimulates the lymphatic system to fight infection, and enhances the immune system; glycyrrhetinic acid inhibits the production and release of immunoglobulin E (IgE) and prostaglandins, regulates immune function, and relieves the toxic side effects of external factors on the skin; ectoine enhances skin immunity; centella asiatica extract promotes barrier damage repair; and ceramides assist in thickening the stratum corneum and repairing skin damage.

[0068] Regarding strengthening the skin's protective barrier, Centella asiatica extract, turmeric extract, tetrahydrocurcumin, glycyrrhetinic acid, and sodium glycinate can improve and restore changes in the appearance and cell structure of skin fibroblasts induced by ultraviolet radiation or external stimuli. Alpha-bisabolol acts on skin exposed to ultraviolet radiation, providing a calming and soothing effect and reducing sunburn redness. Ceramides and ectoine enhance skin hydration, forming a hydration layer to protect the skin and strengthen the skin barrier function.

[0069] To address the issue of ceramides' high crystallinity and incompatibility with essential oils, this application utilizes tetrahydrocurcumin as a "molecular bridge," with the hydrophobic end binding to the alkyl chain of ceramides and the hydrophilic end complexing with the polar groups of guaiac oil, forming a stable complex. This complex maintains stability through hydrophobic interactions, hydrogen bonds, and van der Waals forces, reducing the system's free energy and preventing ceramide crystallization or essential oil stratification.

[0070] Based on the above synergistic effects, the skin repair composition of this application has good transdermal absorption, high skin retention, strong targeting, and is convenient and safe to use. It can solve a variety of skin problems and is suitable for a variety of skin types and usage scenarios.

[0071] A second aspect of this application provides a method for preparing the above-mentioned skin repair composition, comprising:

[0072] S1, α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM and phospholipids are mixed and stirred until melted under an inert atmosphere at a temperature of 45-55℃ and a speed of 800-1200 r / min to obtain the oil phase;

[0073] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate, and soluble zinc salt are added to water and ultrasonically dispersed evenly under an inert atmosphere to obtain an aqueous phase; wherein, the ultrasonic dispersion time is 20~40min and the ultrasonic frequency is 40KHz.

[0074] S3, mix the surfactant and co-surfactant evenly, then add them to the aqueous phase and disperse them by ultrasonication to obtain a mixture; wherein, the ultrasonic dispersion time is 10~20min and the ultrasonic frequency is 40KHz;

[0075] S4. Under stirring, the mixture is slowly added to the oil phase, then recombinant phospholipase A2 is added, and after stirring, homogenization and shearing are performed to obtain the colostrum.

[0076] Among these, a magnetic stirrer is preferably used for stirring, with a stirring speed of 600~700 r / min; the mixture is slowly added to the oil phase at a dropping rate of 1~2 drops per second.

[0077] After adding recombinant phospholipase A2, increase the stirring rate to 1000-1100 r / min and stir for 20-40 min.

[0078] The shearing speed is 8000~12000 rpm, and the shearing time is 5~10 min.

[0079] S5, the colostrum is subjected to a first homogenization process, the product is cooled to 25°C, and then subjected to a second homogenization process to obtain a skin repair composition.

[0080] Specifically, the homogenization process is carried out at a pressure of 30 MPa for 7 to 12 minutes.

[0081] The skin composition obtained by the above preparation method is a nanoemulsion, which not only has high stability and does not separate into layers during high-pressure sterilization or centrifugation, but also has the characteristics of low viscosity, small and uniform particle size, which helps to break the tight structure of the stratum corneum, allowing active ingredients to enter the stratum corneum smoothly, significantly improving the transdermal absorption effect, and has a certain sustained-release and targeted effect; it is both hydrophilic and lipophilic, has a high solubility for active ingredients, and also has certain antibacterial, moisturizing and repairing effects on the skin.

[0082] The skin repair composition of this application has good transdermal absorption, high skin retention, strong targeting, and is convenient and safe to use. It can solve a variety of skin problems, is suitable for a variety of skin types and usage scenarios, and can be used as or in the preparation of skin repair products, such as cosmetics, toners, lotions, etc.

[0083] This application also provides a cosmetic for skin repair, comprising the skin composition of this application, wherein the content is 0.1~10wt%.

[0084] The present application will be further described below through specific embodiments.

[0085] Example 1

[0086] The skin repair composition of Example 1 comprises the following components:

[0087] Centella asiatica extract 3%, ectoine 6%, α-bisabolol 2%, guaiac oil 0.2%, turmeric extract 0.5%, tetrahydrocurcumin 0.4%, glycyrrhetinic acid 0.7%, sodium gluconate 0.15%, ceramide 3%, soybean lecithin 10%, hydrogenated lecithin 5%, structured lipid MCM 6%, Tween 20 4%, PEG400 20%, 1,2-propylene glycol 5%, zinc lactate 0.1%, recombinant phospholipase A2 0.04%, balance ultrapure water.

[0088] Its preparation methods include:

[0089] S1, mix α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM, soybean lecithin, and hydrogenated lecithin evenly, and stir at 1000 r / min for 15 minutes under nitrogen atmosphere and 50℃ to completely melt it, to obtain the oil phase and keep it warm for later use.

[0090] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate and zinc lactate were added to water and ultrasonically dispersed for 30 min at an ultrasonic frequency of 40 kHz under a nitrogen atmosphere to obtain an aqueous phase;

[0091] S3, mix Tween 20, PEG400 and 1,2-propylene glycol evenly, then add to the aqueous phase, set the ultrasonic frequency to 40kHz, and ultrasonically disperse for 30min to obtain a mixture;

[0092] S4. Under a magnetic stirrer with a stirring speed of 600 r / min, the mixture is slowly added to the oil phase at a dropping rate of 1-2 drops per second, and then recombinant phospholipase A2 is added. The stirring speed is increased to 1000 r / min and stirred for 30 min. Then, the mixture is homogenized and sheared to obtain the colostrum. The shearing speed is 10000 rpm and the shearing time is 8 min.

[0093] S5, the colostrum is homogenized using a high-speed homogenizer at a pressure of 30 MPa for 10 min; the product is cooled to 25°C and then homogenized a second time to obtain a skin repair composition nanoemulsion.

[0094] Example 2

[0095] The skin repair composition of Example 2 comprises the following components:

[0096] Centella asiatica extract 2%, ectoine 4%, α-bisabolol 3%, guaiac oil 0.4%, turmeric extract 0.8%, tetrahydrocurcumin 0.3%, glycyrrhetinic acid 0.8%, sodium gluconate 0.1%, ceramide 2%, cephalin 10%, sphingomyelin 8%, structured lipid MCM 6%, castor oil polyoxyethylene ether 40 5%, glycerol 5%, 1,3-butanediol 5%, zinc lactate 0.06%, recombinant phospholipase A2 0.08%, balance ultrapure water.

[0097] Its preparation methods include:

[0098] S1, α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM, cerebroside, and sphingomyelin are mixed evenly and stirred at 900 r / min for 18 minutes under a nitrogen atmosphere and at 52°C to completely melt the mixture, and the oil phase is kept warm for later use.

[0099] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate and zinc lactate were added to water and ultrasonically dispersed for 30 min at an ultrasonic frequency of 40 kHz under a nitrogen atmosphere to obtain an aqueous phase;

[0100] S3, castor oil polyoxyethylene ether 40, glycerol and 1,3-butanediol are mixed evenly, and then added to the aqueous phase. The ultrasonic frequency is set to 40kHz and ultrasonically dispersed for 10min to obtain a mixture.

[0101] S4. Under the magnetic stirrer with a stirring speed of 600 r / min, the mixture is slowly added to the oil phase at a dropping rate of 1-2 drops per second, and then recombinant phospholipase A2 is added. The stirring speed is increased to 1000 r / min and stirred for 35 min. Then, the mixture is homogenized and sheared to obtain the colostrum. The shearing speed is 9000 rpm and the shearing time is 9 min.

[0102] S5, the colostrum is homogenized using a high-speed homogenizer at a pressure of 30 MPa for 8 minutes; the product is cooled to 25°C and then homogenized a second time to obtain a skin repair composition nanoemulsion.

[0103] Example 3

[0104] The skin repair composition of Example 3 comprises the following components:

[0105] Centella asiatica extract 5%, ectoine 8%, α-bisabolol 1%, guaiac oil 0.5%, turmeric extract 1%, tetrahydrocurcumin 0.2%, glycyrrhetinic acid 0.1%, sodium gluconate 0.01%, ceramide 0.1%, egg yolk lecithin 10%, structured lipid MCM 8%, polyglycerol-10 oleate 10%, polypropylene glycol 10%, ethoxydiethylene glycol 10%, glycerol polyether-26 10%, zinc lactate 0.05%, recombinant phospholipase A2 0.01%, balance ultrapure water.

[0106] Its preparation methods include:

[0107] S1, mix α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM and egg yolk lecithin evenly, stir at 1200 r / min for 10 minutes under nitrogen atmosphere and 45℃ to completely melt it, and keep it warm for later use.

[0108] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate and zinc lactate were added to water and ultrasonically dispersed for 40 min under a nitrogen atmosphere to obtain an aqueous phase.

[0109] S3, mix polyglycerol-10 oleate with 10% polypropylene glycol, 10% ethoxydiethylene glycol and 10% glycerol polyether-26 evenly, then add to the aqueous phase, set the ultrasonic frequency to 40 kHz, and ultrasonically disperse for 10 min to obtain a mixture;

[0110] S4. Under the magnetic stirrer with a stirring speed of 600 r / min, the mixture is slowly added to the oil phase at a dropping rate of 1 to 2 drops per second, and then recombinant phospholipase A2 is added. The stirring speed is increased to 1000 r / min and stirred for 20 min. Then, the mixture is mixed, homogenized and sheared to obtain the colostrum. The shearing speed is 12000 rpm and the shearing time is 5 min.

[0111] S5, the colostrum is homogenized using a high-speed homogenizer at a pressure of 30 MPa for 12 minutes; the product is cooled to 25°C and then homogenized a second time to obtain a skin repair composition nanoemulsion.

[0112] Example 4

[0113] The skin repair composition of Example 4 comprises the following components:

[0114] Centella asiatica extract 0.5%, ectoine 1%, α-bisabolol 3%, guaiac oil 0.1%, turmeric extract 0.03%, tetrahydrocurcumin 0.5%, glycyrrhetinic acid 1%, sodium gluconate 0.2%, ceramide 1%, hydrogenated lecithin 10%, egg yolk lecithin 10%, structured lipid MCM 3%.

[0115] The composition consists of 3% polyoxyethylene hydrogenated castor oil, 3% polyglycerol-10 laurate, 7% ethoxydiethylene glycol, 8% 1,3-butanediol, 0.15% zinc lactate, 0.1% recombinant phospholipase A2, and the balance being ultrapure water.

[0116] Its preparation methods include:

[0117] S1, mix α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM and hydrogenated lecithin 10% egg yolk lecithin evenly, stir at 800 r / min for 20 minutes under nitrogen atmosphere and 55℃ to completely melt it, and keep it warm for later use.

[0118] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate and zinc lactate were added to water and ultrasonically dispersed for 20 min at an ultrasonic frequency of 40 kHz under a nitrogen atmosphere to obtain an aqueous phase;

[0119] S3, mix polyoxyethylene hydrogenated castor oil, polyglycerol-10 laurate, ethoxydiethylene glycol, and 1,3-butanediol evenly, then add them to the aqueous phase, set the ultrasonic frequency to 40 kHz, and ultrasonically disperse for 20 min to obtain a mixture.

[0120] S4. Under the magnetic stirrer with a stirring speed of 600 r / min, the mixture is slowly added to the oil phase at a dropping rate of 1 to 2 drops per second, and then recombinant phospholipase A2 is added. The stirring speed is increased to 1000 r / min and stirred for 40 min. Then, the mixture is mixed, homogenized and sheared to obtain the colostrum. The shearing speed is 8000 rpm and the shearing time is 10 min.

[0121] S5, the colostrum is homogenized using a high-speed homogenizer at a pressure of 30 MPa for 7 minutes; the product is cooled to 25°C and then homogenized a second time to obtain a skin repair composition nanoemulsion.

[0122] Comparative Example 1

[0123] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain structured lipid MCM, but instead uses glyceryl monostearate to replace the structured lipid MCM, while the rest is the same as Example 1.

[0124] Comparative Example 2

[0125] The difference between Comparative Example 2 and Example 1 is that there is no soluble zinc salt in Comparative Example 2, while all other aspects are the same as in Example 1.

[0126] Comparative Example 3

[0127] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain recombinant phospholipase A2 (rPLA2), while all other aspects are the same as in Example 1.

[0128] Comparative Example 4

[0129] The difference between Comparative Example 4 and Example 1 is that nanoemulsion technology was not used.

[0130] Comparative Example 4's skin repair composition comprises the following components:

[0131] Centella asiatica extract 3%, ectoine 6%, α-bisabolol 2%, guaiac oil 0.2%, turmeric extract 0.5%, tetrahydrocurcumin 0.4%, glycyrrhetinic acid 0.7%, sodium gluconate 0.15%, ceramide 3%, soybean lecithin 10%, hydrogenated lecithin 5%, structured lipid MCM 6%, zinc lactate 0.1%, balance ultrapure water.

[0132] Its preparation methods include:

[0133] S1, mix α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM, soybean lecithin, and hydrogenated lecithin evenly, and stir at 1000 r / min for 15 minutes under nitrogen atmosphere and 50℃ to completely melt it, to obtain the oil phase and keep it warm for later use.

[0134] S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate and zinc lactate were added to water and ultrasonically dispersed for 30 min at an ultrasonic frequency of 40 kHz under a nitrogen atmosphere to obtain an aqueous phase;

[0135] S3. Mix Tween 20, PEG400 and 1,2-propylene glycol evenly, then add them to the aqueous phase, set the ultrasonic frequency to 40 kHz, and ultrasonically disperse for 30 min to obtain a mixture.

[0136] The performance of the skin repair composition emulsions prepared in the embodiments and comparative examples of this application was evaluated as follows:

[0137] 1. Stability Testing

[0138] The samples prepared in Examples 1-4 and Comparative Examples 2-4 of this application were placed in sealed containers and stored at -18°C, 4°C, room temperature, and 45°C for 90 days each. The samples were examined at room temperature after the initial condition and 90 days to check for precipitation or stratification, and the particle size was measured. The test results are shown in Table 1. A demulsifier (methanol / acetonitrile, volume ratio 1:1) was used for treatment, and the retention rate of the active ingredient was analyzed and calculated by high-performance liquid chromatography.

[0139] Table 1. Stability test results of samples from the examples and comparative examples.

[0140]

[0141] As shown in Table 1, the samples from Examples 1-4 and Comparative Examples 2-3 all exhibited good stability. After being placed at -18℃, 4℃, room temperature, and 45℃ for 90 days, no precipitation or stratification occurred, the particle size change was small, the uniformity of the nanoemulsion dispersion system was maintained, and no leakage of active ingredients was observed, ensuring efficacy and providing convenient storage. Specifically, the products prepared in Examples 1-4 had smaller particle sizes, smaller particle size variations, and more stable systems than those in Comparative Examples 2-4, with active ingredient retention rates all exceeding 96%. Comparative Example 4, prepared using the nanoemulsion technology of this application, presented a suspension state with poor stability, and the active ingredient retention rate was significantly lower than other samples. This indicates that the preparation process of this application is more advantageous.

[0142] 2. Anti-inflammatory capacity test

[0143] HaCaT cells with a confluence of 80-90% were treated with digestion solution, centrifuged, and then seeded into 6-well plates and incubated for 24 h. Once the cell deposition rate in the plates met the testing requirements, negative control, positive control, and test groups were set up for experiments. The negative control group received maintenance culture medium, the positive control group received maintenance culture medium containing 1 ng / mLEGF, and the test group received cell culture medium containing samples from Examples 1-4 and Comparative Examples 1-4 (diluted to 1%). All samples were diluted with purified water. Each sample group had three replicate wells, and incubation continued for 24 h. After incubation, the culture medium was aspirated from the plates, and 1 mL of PBS was added to each well. The culture was then subjected to a pulse at an intensity of 30 mJ / cm². 2 All samples were irradiated with UVB ultraviolet light for 40 min, then the liquid in the sample wells was discarded and cell culture medium was added back in. The samples were cultured for another 24 h, and the cell supernatant was collected. TNF-α and IL-6 levels were detected according to the ELISA kit instructions. The results are as follows: Figure 1 and Figure 2 As shown.

[0144] from Figure 1 and Figure 2It was found that UVB light induced a large expression of inflammatory factors (TNF-α, IL-6) in HaCaT cells, while the positive control group and Examples 1-4 all significantly reduced the expression of inflammatory factors in HaCaT cells, with statistically significant differences (P < 0.01). Furthermore, the effects of the examples were superior to those of the control group. These results indicate that the samples from Examples 1-4 of this application can significantly reduce the expression of cellular inflammatory factors, alleviate inflammatory responses, and improve skin health.

[0145] 3. Protective effect against H2O2-induced oxidative damage to HaCaT cells

[0146] HaCaT cells with a confluence of 80-90% were treated with digestion solution and then centrifuged at 5 × 10⁻⁶. 4 Cells were seeded at a density of cells / well in 96-well plates and cultured for 24 h. Experimental and control groups were set up. The experimental groups were supplemented with samples from Examples 1-4 and Comparative Examples 1-4 diluted to 1%. After 24 h, the drug culture medium was removed, the cells were washed once with PBS, and then 300 μM hydrogen peroxide was added. The control group did not receive hydrogen peroxide but received an equal volume of culture medium. After 4 h, the culture medium was removed, the cells were washed once with PBS, and then culture medium containing 10% CCK8 was added. After incubation for 1 h, the absorbance at 450 nm was measured using a microplate reader. The results are as follows: Figure 3 As shown.

[0147] Depend on Figure 3 It was found that after hydrogen peroxide damage, the cell viability in the model group was 18%, indicating that the hydrogen peroxide-damaged HaCaT model was successfully established. Compared with the blank group, the products prepared in Examples 1-4 all significantly improved the viability of oxidatively damaged HaCaT cells, and the differences were statistically significant (P < 0.01). Among them, the product prepared in Example 3 had the strongest anti-oxidative damage performance. The products prepared in the examples of this application all had better effects than the comparative examples, indicating that the samples prepared in this application can help the skin resist photodamage and photoaging caused by sunlight exposure.

[0148] 4. 24-hour closed patch test on human skin

[0149] Thirty subjects who met the voluntary inclusion criteria were recruited. Using qualified patch testing equipment, a closed patch test method was employed. Approximately 0.020g to 0.025g of the test sample (Examples 1-4, Comparative Examples 1-4) was placed in the patch device, and a hypoallergenic adhesive tape was applied to the flexor side of the subject's forearm. The patch device was removed after 24 hours, and skin reaction was observed 0.5 hours after removal. Skin reaction was observed again 24 hours after patch removal. A negative control was used as a blank control. The results were recorded according to the skin reaction grading standards in LABC-WI-HZ-033 "Instructions for 24-Hour Closed Patch Testing of Human Skin," as shown in Table 2.

[0150] Table 2 Results of 24-hour closed patch test on human skin

[0151]

[0152] Human skin patch testing reproduces the reaction process of allergic contact dermatitis on local skin. As shown in Table 2, after 24 hours of contact with human skin, 0 out of 33 people experienced adverse reactions, with no erythema, swelling, or other symptoms. The results indicate that the nanoemulsion of the repair composition described in this application is mild and low-irritant to the skin, demonstrating high safety.

[0153] 5. Human efficacy testing

[0154] Volunteers were recruited for human efficacy testing. Participants were required to have no history of allergies, good skin condition, and not have taken any medications that might affect the efficacy before the test. Thirty volunteers were recruited (10 males and 20 females), all meeting the inclusion criteria. Before the test, blank control and sample application groups were designated, and 3cm × 3cm markings were made in the corresponding test areas. Skin moisture content was measured using a Moisturemeter, and skin elasticity (R7 value) was measured using a Skin Elastomer meter as baseline values. Examples 1-4 and Comparative Examples 1-3 were evenly applied (2.0 ± 0.1 g) / cm² to the test areas. 2 No other products were allowed in the blank control and test areas. The moisture content and elasticity values ​​of the control and test groups were measured at different time points after application (14 days and 28 days) to examine the effect of the test sample on skin moisture content and skin elasticity. The stratum corneum moisture value reflects the overall moisture content of the skin, while skin elasticity is an indicator of skin health, used to assess skin firmness and suppleness. The data shown are the average test data of all subjects. Results are as follows... Figure 4 , Figure 5 As shown.

[0155] from Figure 4 , Figure 5It can be seen that after 28 days of continuous use, the moisture content of the stratum corneum of the skin in all examples increased significantly, with Example 1 showing the highest increase. Although Comparative Examples 1-3 also improved the moisture content of the stratum corneum, the increase was less than that in Examples 1-3. The repair nanoemulsion provided in this application can enhance the skin's hydration capacity and provide a stable and long-lasting moisturizing effect.

[0156] After 28 days of continuous use, skin elasticity significantly improved in all cases, with the highest increase observed in the examples. While comparative examples 1-3 also showed some improvement in skin elasticity, the increase was less pronounced than in the examples. The repair nanoemulsion provided in this application can tighten the skin, improve skin plumpness, and enhance the overall appearance of the skin.

[0157] Although this application has been described in detail in this specification with general descriptions and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, such modifications or improvements made without departing from the spirit of this application are all within the scope of protection claimed in this application.

Claims

1. A skin repair composition, characterized in that, It comprises the following components by weight percentage: Centella asiatica extract 0.5%~5%, ectoine 1%~8%, α-bisabolol 1%~3%, guaiac oil 0.1%~0.5%, turmeric extract 0.03%~1%, tetrahydrocurcumin 0.2%~0.5%, glycyrrhetinic acid 0.1%~1%, sodium glycinate 0.01%~0.2%, ceramide 0.1%~3%, phospholipids 10%~20%, structured lipid MCM 3%~8%, surfactant 2%~6%, cosurfactant 15%~30%, soluble zinc salt 0.05%~0.15%, recombinant phospholipase A2 0.01%~0.1%, balance ultrapure water.

2. The skin repair composition according to claim 1, characterized in that, The structured lipid MCM is a mannose-conjugated medium-chain triglyceride derivative.

3. The skin repair composition according to claim 2, characterized in that, The mannose conjugated medium-chain triglyceride derivative has a triglyceride core backbone, an ω-3 polyunsaturated fatty acid chain, and terminal mannose modification; the ω-3 polyunsaturated fatty acid chain is eicosapentaenoic acid.

4. The skin repair composition according to claim 1, characterized in that, The soluble zinc salt is zinc lactate.

5. The skin repair composition according to claim 1, characterized in that, The phospholipids include at least one of soybean lecithin, cephalin, sphingomyelin, hydrogenated lecithin, or egg yolk lecithin.

6. The skin repair composition according to claim 1, characterized in that, The surfactant is at least one of Tween 20, castor oil polyoxyethylene ether 40, Span 80, sorbitan monooleate, polyglycerol-10 laurate, polyglycerol-10 oleate, or polyoxyethylene hydrogenated castor oil. The co-surfactant is at least one of PEG400, 1,2-propylene glycol, glycerin, 1,3-butanediol, polypropylene glycol, ethoxydiethylene glycol, or glycerol polyether-26.

7. The method for preparing the skin repair composition according to claim 1, characterized in that, include: S1, α-bisabolol, guaiac oil, turmeric extract, glycyrrhetinic acid, ceramide, structured lipid MCM and phospholipids are mixed and stirred until melted under an inert atmosphere to obtain the oil phase; S2, Centella asiatica extract, tetrahydrocurcumin, ectoine, sodium gluconate, and soluble zinc salt were added to water and ultrasonically dispersed uniformly under an inert atmosphere to obtain an aqueous phase; S3, mix the surfactant and co-surfactant evenly, then add them to the aqueous phase and disperse by ultrasonication to obtain a mixture; S4. Under the magnetic stirrer, the mixture is slowly added to the oil phase, then recombinant phospholipase A2 is added, and after stirring, homogenization and shearing are performed to obtain the colostrum. S5, the colostrum is subjected to a first homogenization process, the product is cooled to 25°C, and then subjected to a second homogenization process to obtain a skin repair composition.

8. The preparation method according to claim 7, characterized in that, Stirring is carried out in S1 at 45-55℃; In S2, the ultrasonic dispersion time is 20~40 min, and the ultrasonic frequency is 40KHz; In S3, the ultrasonic dispersion time is 10~20 min, and the ultrasonic frequency is 40KHz; In S4, the mixture is slowly added to the oil phase at a dropping rate of 1-2 drops per second; the stirring rate is 1000 r / min and the stirring time is 20-40 min; the shearing speed is 8000-12000 rpm and the shearing time is 5-10 min. In S5, the homogenization process is carried out at a pressure of 30 MPa for 7 to 12 minutes.

9. Use of the skin repair composition according to any one of claims 1-8 in a product for skin repair.

10. A cosmetic product for skin repair, characterized in that, It contains 0.1 to 10 wt% of the skin repair composition as described in any one of claims 1-8.

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