Sensitive skin care composition with soothing, repairing efficacy and use thereof

Abstract

The present application relates to a kind of sensitive skin care composition with soothing, repair effect and its application, it is composed of 4-tert-butyl cyclohexanol, schisandra chinensis extract and grey hair bean seed extract, the mass ratio of 4-tert-butyl cyclohexanol, schisandra chinensis extract and grey hair bean seed extract is 1:(0.01-0.1):(0.01-0.1);Wherein, schisandra chinensis extract and grey hair bean seed extract are obtained using enzymatic hydrolysis-fermentation process, the composition of the present application has good soothing repair effect, and the active ingredient combination shows good compatibility and stability in formula system, without additional solubilizer and emulsifier, adapt more mild dosage form, more fit the needs of moderate sensitive skin.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a sensitive skin care composition with soothing and repairing effects and application thereof. BACKGROUND

[0002] In today's society, people generally bear a lot of work and study pressure. Long time in this high pressure state, combined with the continuous change and stimulation of the external climate environment, can lead to abnormal secretion of sebaceous glands and sweat glands of the skin. With age, the skin of a person will gradually age, and under the combined action of stress and environmental factors, the secretion of sebum is reduced, and the water retention capacity of the skin is also reduced, thereby causing the skin to become dry. The stratum corneum of the skin epidermis will gradually thicken, causing skin keratinization, cracking, and a series of problems such as rough skin surface. In recent years, with the continuous improvement of people's beauty consciousness, how to effectively repair and moisturize has become one of the current research hotspots in the field of skin care.

[0003] CN117357460B discloses a soothing special care composition comprising 4-tert-butylcyclohexanol, schisandra chinensis extract and milk vetch seed extract. Among them, 4-tert-butylcyclohexanol is a typical liposoluble ingredient (logP≈3.5, almost insoluble in water, easily soluble in ethanol, oil), and the residual polysaccharide and protein in schisandra chinensis extract have strong hydrophilicity, and milk vetch seed extract also contains hydrophilic components, so there is a polarity conflict between 4-tert-butylcyclohexanol, schisandra chinensis extract and milk vetch seed extract. In order to maintain compatibility, CN117357460B adds a large amount of oil components (squalane, muru muru butter) and polyols (1,2-pentanediol), which leads to inherent limitations of the formula: the increase of oil components increases the sticky feeling of the formula, which is only suitable for emulsion, cream and other oily bases, and cannot develop alcohol-free, refreshing formulations (such as toner, essence) required by sensitive skin.

[0004] CN116712342B also discloses a soothing special care composition comprising 4-tert-butylcyclohexanol, schisandra chinensis extract and milk vetch seed extract. It also faces the problem of poor compatibility of 4-tert-butylcyclohexanol, schisandra chinensis extract and milk vetch seed extract. The solution of CN116712342B is to use 4-tert-butylcyclohexanol as a capsule shell material, and schisandra chinensis extract and milk vetch seed extract as the encapsulated contents. Because it relies on a vesicular structure, the formula needs to contain a large amount of non-ionic surfactant and cholesterol, which can only be adapted to emulsion, cream and other oily bases, and the high proportion of capsule material limits the space for adding active ingredients. SUMMARY

[0005] The present application aims to overcome the deficiencies of the prior art and provide a sensitive skin care composition with soothing and repairing effects and its application. The two plant fermentation extracts and 4-tert-butylcyclohexanol have good compatibility, do not require a large amount of emulsifier or the preparation of microcapsule form, are easier to apply to various dosage forms (such as toner, serum) of water-based, alcohol-free, and refreshing types, and can adjust the formula texture according to the sensitive skin type (dry sensitive skin, oily sensitive skin).

[0006] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is:

[0007] In a first aspect, the present application provides a sensitive skin care composition with soothing and repairing effects, which is composed of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Millettia pachycarpa seed extract, and the mass ratio of the 4-tert-butylcyclohexanol, Schisandra chinensis extract and Millettia pachycarpa seed extract is 1: (0.01-0.1): (0.01-0.1).

[0008] The preparation method of the Schisandra chinensis extract includes the following steps:

[0009] S1, grinding Schisandra chinensis, adding 5-6 times the mass of water, adjusting the pH to 5.5-6.5, adding a first composite enzyme for enzymolysis, inactivating the enzyme, and cooling to room temperature to obtain an enzyme solution; wherein the first composite enzyme includes cellulase, pectinase and papain;

[0010] S2, sterilizing the enzyme solution obtained in step S1, then inoculating a first composite fermentation bacteria solution in the enzyme solution for fermentation, sterilizing, filtering to obtain a fermentation liquid and a fermentation residue; wherein the first composite fermentation bacteria solution includes Phellinus baumii, Geotrichum candidum and Lactobacillus buchneri;

[0011] S3, adding the fermentation residue obtained in step S2 to 3-4 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuging, and collecting the supernatant;

[0012] S4, mixing the fermentation liquid obtained in step S2 and the supernatant collected in step S3, freeze-drying to obtain the Schisandra chinensis extract;

[0013] The preparation method of the Millettia pachycarpa seed extract includes the following steps:

[0014] T1, grinding Millettia pachycarpa seeds, adding 5-6 times the mass of water, adjusting the pH to 5.5-6.5, adding a second composite enzyme for enzymolysis, inactivating the enzyme, and cooling to room temperature to obtain an enzyme solution; wherein the second composite enzyme is papain and cellulase;

[0015] T2, sterilizing the enzyme solution obtained in step T1, then inoculating the second complex fermentation bacteria solution into the enzyme solution to perform fermentation, sterilizing, filtering, and obtaining fermentation liquor and fermentation residue; wherein the second complex fermentation bacteria solution comprises Aspergillus coronatus and Candida lipolytica;

[0016] T3, adding the fermentation residue obtained in step T2 into 3-4 times mass of anhydrous ethanol to perform ultrasonic extraction, centrifuging, and collecting the supernatant;

[0017] T4, mixing the fermentation liquor obtained in step T2 and the supernatant collected in step T3, and freeze-drying to obtain the greyhairy soybean seed extract.

[0018] The composition of the present application only contains 4-tert-butylcyclohexanol, schisandra chinensis extract and greyhairy soybean seed extract, wherein the schisandra chinensis extract and the greyhairy soybean seed extract are obtained by the method of enzymolysis-fermentation-alcohol extraction-drying, so that the schisandra chinensis extract and the greyhairy soybean seed extract have good compatibility with 4-tert-butylcyclohexanol, and no emulsifier and solubilizer needs to be added in the composition, and the preparation principle of the schisandra chinensis extract and the greyhairy soybean seed extract is as follows:

[0019] In the preparation step S1 of the schisandra chinensis extract, the first complex enzyme including cellulase, pectinase and papain is used for enzymolysis of schisandra chinensis, through the synergistic effect of cellulase for degrading cell wall cellulose, pectinase for degrading pectin and papain for degrading structural protein, not only the cell wall and protein network of schisandra chinensis are broken, and the active ingredients wrapped are released, but also the hydrophilic impurities in the extract, i.e. cellulose and pectin (strongly hydrophilic polysaccharide) are efficiently removed, and the cellulose and pectin are degraded into small molecular monosaccharides such as glucose and galacturonic acid, and the structural protein is hydrolyzed into small molecular short peptides and amino acids, avoiding the oil-water separation of macromolecular protein and 4-tert-butylcyclohexanol. In the preparation step S2 of the schisandra chinensis extract, the first complex fermentation bacteria solution is used for fermentation of the enzyme solution, wherein the myceliopthora verrucosa can metabolize to produce polysaccharides and flavonoids, which are superimposed with the active ingredients of schisandra chinensis itself, to enhance the antioxidant capacity; the geotrichum candidum can convert the low-activity lignan precursors in schisandra chinensis into high-activity lignan derivatives (such as schisantherin A / B), to improve the anti-inflammatory effect of the composition; the lactobacillus buchneri ferments to produce lactic acid and short-chain fatty acids, which on the one hand regulate the pH of the extraction system, promote the stable dissolution of active ingredients, and on the other hand, lactic acid can assist the metabolism of the cuticle layer of the skin, to improve the penetration and absorption efficiency of the ingredients. And the short-chain fatty acids have both hydrophilic groups (such as hydroxyl and carboxyl) and lipophilic groups (such as alkyl), which can be used as amphiphilic surfactants, can be adsorbed and arranged on the oil-water interface, so that the fat-soluble ingredients can be stably dispersed in the water-soluble system, to improve the compatibility of the composition system.

[0020] The liposoluble active ingredients remaining in the fermentation residue in the preparation step S3 of the Schisandra chinensis extract are enriched again by ultrasonic extraction with anhydrous ethanol, further improving the proportion of liposoluble ingredients in the extract and making the composition more compatible with the 4-tert-butylcyclohexanol; the freeze-drying in the preparation step S4 avoids the agglomeration of ingredients caused by high-temperature drying, and the final extract is a loose and porous powder rather than a dense and massive solid as in the alcohol extract, which is easy to disperse in the formula and can quickly form a uniform suspension system when mixed with 4-tert-butylcyclohexanol, without the need for a large amount of solubilizer.

[0021] In the preparation step T1 of the Millettia pachycarpa seed extract, the second complex enzyme of papain and cellulase is selected because the cell wall of the Millettia pachycarpa seed is more tightly combined with the protein network, papain preferentially degrades seed proteins, converting them into small molecular peptides and releasing the isoflavones wrapped in proteins, and avoiding the polarity conflict between macromolecular proteins and 4-tert-butylcyclohexanol; cellulase degrades cell wall cellulose, assisting in the release of wrapped liposoluble ingredients and reducing the residual hydrophilic polysaccharides.

[0022] In the preparation step T2, Aspergillus coronatus (fungi) and Candida lipolytica (yeast) are used to synergistically ferment the enzymatic hydrolysate, β-glucosidase produced by Aspergillus coronatus hydrolyzes isoflavone glycosides into aglycones, the liposolubility of which is highly compatible with 4-tert-butylcyclohexanol, solving the problem of excessive hydrophilicity of glycosides; Candida lipolytica can metabolize fatty acids (such as oleic acid, linoleic acid) and lipase, fatty acids can form a mixed oil phase with 4-tert-butylcyclohexanol, and lipase further promotes the dispersion of liposoluble ingredients. Moreover, the polysaccharide derivatives secreted by Aspergillus coronatus and the fatty acids produced by Candida lipolytica have natural emulsifying properties, further improving the compatibility of the composition. In the preparation step T3, the isoflavone aglycone (liposoluble) remaining in the fermentation residue is fully extracted by ultrasonic extraction with anhydrous ethanol, increasing the proportion of isoflavone aglycone in the Millettia pachycarpa seed extract and further strengthening the liposolubility compatibility with 4-tert-butylcyclohexanol.

[0023] Preferably, the mass ratio of the 4-tert-butylcyclohexanol, the Schisandra chinensis extract, and the Millettia pachycarpa seed extract is 1: (0.02-0.04): (0.06-0.08).

[0024] Preferably, in the preparation step S1 of the Schisandra extract, the cellulase is added in an amount of 0.3-0.6% of the mass of the Schisandra, and the cellulase has an enzyme activity of 50000-100000 U / g; the pectinase is added in an amount of 0.2-0.5% of the mass of the Schisandra, and the pectinase has an enzyme activity of 50000-100000 U / g; the papain is added in an amount of 0.1-0.5% of the mass of the Schisandra, and the papain has an enzyme activity of 100000-200000 U / g; the enzymolysis is performed at a temperature of 40-45℃ for 1-2h; and the enzyme is inactivated at a temperature of 95-105℃ for 15-25min.

[0025] Preferably, in the preparation step S2 of the Schisandra extract, the first compound fermentation bacterial liquid is inoculated in an amount of 2-7% v / v, and the total viable bacterial count of the first compound fermentation bacterial liquid is (1-3)×10 9 CFU / mL, the viable bacterial count ratio of the Phellinus igniarius, Geotrichum candidum and Lactobacillus buchneri is (0.5-1):1:(1.5-2.5), and the fermentation is performed at a temperature of 30-35℃ for 24-72h.

[0026] Preferably, in the preparation step S3 of the Schisandra extract, the ultrasonic extraction is performed at a temperature of 35-45℃, a power of 100-300W, and for 20-40min.

[0027] Preferably, in the preparation step T1 of the greyhairy soybean seed extract, the cellulase is added in an amount of 0.2-0.5% of the mass of the greyhairy soybean seed, and the cellulase has an enzyme activity of 50000-100000 U / g; the papain is added in an amount of 0.2-0.5% of the mass of the greyhairy soybean seed, and the papain has an enzyme activity of 100000-200000 U / g; and the enzymolysis is performed at a temperature of 50-60℃ for 1-2h.

[0028] Preferably, in the preparation step T2 of the greyhairy soybean seed extract, the second compound fermentation bacterial liquid is inoculated in an amount of 3-5% v / v, and the total viable bacterial count of the second compound fermentation bacterial liquid is (1-3)×10 9 CFU / mL, the viable bacterial count ratio of the Aspergillus corntus and Candida lipolytica is (0.5-1.5):1, and the fermentation is performed at a temperature of 30-35℃ for 24-72h.

[0029] Preferably, in the preparation step T3 of the greyhairy soybean seed extract, the ultrasonic extraction is performed at a temperature of 35-45℃, a power of 100-300W, and for 20-40min.

[0030] In a second aspect, the present application provides the use of the sensitive skin care composition with soothing and repairing effects in the first aspect in the preparation of skin cosmetic products.

[0031] In a third aspect, a skin cosmetic product comprises the sensitive skin care composition with soothing and repairing efficacy in the first aspect.

[0032] Preferably, the skin cosmetic product comprises the following mass percentage of components: the sensitive skin care composition with soothing and repairing efficacy in the first aspect 0.1-5%, a cosmetic base 2-40%, and the balance water.

[0033] Preferably, the skin cosmetic product comprises any one of a cosmetic water, a cream, a mask, a serum, an essence oil, and a spray.

[0034] Compared with the prior art, the present application has the following advantages:

[0035] The composition of the present application contains 4-tert-butylcyclohexanol and Schisandra chinensis extract and Millettia pinnata seed extract obtained by a specific preparation method. The Schisandra chinensis extract and Millettia pinnata seed extract are both obtained by an enzymatic hydrolysis-fermentation process. The enzymatic hydrolysis process degrades strong hydrophilic impurities, reduces the polarity of the extract, and narrows the polarity difference with 4-tert-butylcyclohexanol. The fermentation converts the active ingredients into small liposoluble molecules, matches the solubility of 4-tert-butylcyclohexanol, and forms a self-emulsifying system with organic acids, amino acids, etc. in the fermentation product, thereby fundamentally solving the compatibility problem of 4-tert-butylcyclohexanol and hydrophilic extracts. The secondary extraction enriches the liposoluble components, further improving the compatibility of the Schisandra chinensis extract and Millettia pinnata seed extract with 4-tert-butylcyclohexanol. The freeze-drying process produces a loose and porous powder that is easy to disperse into a uniform suspension system. The composition of the present application has good soothing and repairing effects, and can also fundamentally solve the compatibility problem without the need for additional solubilizers and emulsifiers, which is suitable for more mild dosage forms and better meets the needs of moderate to severe sensitive skin. BRIEF DESCRIPTION OF DRAWINGS

[0036] Figure 1 Zebrafish tail region fluorescence images of the compositions of Example 1 and Comparative Example 1;

[0037] Figure 2 Human patch situation images of Application Examples 1-7 and Comparative Application Examples 1-10;

[0038] Figure 3 Physical images of the serums of Application Example 1, Comparative Application Example 1, and Comparative Application Example 10. DETAILED DESCRIPTION

[0039] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in conjunction with specific examples.

[0040] The raw materials used in the following examples and comparative examples are as follows:

[0041] Schisandra sphenanthera: the manufacturer is Hunan Songlingtang Traditional Chinese Medicine Decoction Co., Ltd., and the trade name is Schisandra sphenanthera;

[0042] Sanghuangporus vaninii: purchased from China General Microbiological Culture Collection Center, and the preservation number is CGMCC 5.891;

[0043] Geotrichum candidum: purchased from China General Microbiological Culture Collection Center, and the preservation number is CGMCC 2.1135;

[0044] Lactobacillus buchneri: purchased from China General Microbiological Culture Collection Center, and the preservation number is CGMCC 1.13;

[0045] Tephrosia purpurea seeds: collected in Honghe Hani and Yi Autonomous Prefecture, Yunnan Province;

[0046] Aspergillus corallinus: the manufacturer is Beijing Baoewei Biotechnology Co., Ltd., and the model is Bio-104127;

[0047] Candida lipolytica: purchased from China Industrial Microbial Culture Collection Center, and the preservation number is CICC 32696;

[0048] 4-tert-butylcyclohexanol: the manufacturer is Symrise (Shanghai) Co., Ltd., and the model is SymSitive ® 1609;

[0049] The commercially available control samples used in the comparative examples are as follows:

[0050] Schisandra sphenanthera extract: the manufacturer is Shanghai Bosik Industry Co., Ltd., and the model is Schisandra sphenanthera extract;

[0051] Tephrosia purpurea seed extract: the manufacturer is Kewei Company, and the model is NEUROPHROLINE product;

[0052] Other materials, reagents, etc. used in the examples and comparative examples can be obtained from commercial channels, unless otherwise specified.

[0053] The preparation method of Schisandra sphenanthera extract and Tephrosia purpurea seed extract in the sensitive skin care composition with soothing and repairing effects of Examples 1-5 is as follows:

[0054] The preparation method of the Schisandra sphenanthera extract comprises the following steps:

[0055] S1, the Schisandra sphenanthera is crushed, 5 times the mass of water is added, the pH is adjusted to 6, the first composite enzyme is added for enzymolysis, the enzyme is inactivated, and it is cooled to room temperature to obtain an enzymolysis liquid; wherein the first composite enzyme comprises cellulase, pectinase and papain, the cellulase is added in an amount of 0.5% of the mass of the Schisandra sphenanthera, the cellulase has an enzyme activity of 80000 U / g; the pectinase is added in an amount of 0.4% of the mass of the Schisandra sphenanthera, the pectinase has an enzyme activity of 90000 U / g; the papain is added in an amount of 0.2% of the mass of the Schisandra sphenanthera, the papain has an enzyme activity of 150000 U / g; the enzymolysis temperature is 43 DEG C, the time is 1.5 h, the inactivation temperature is 100 DEG C, and the inactivation time is 20 min;

[0056] S2, the enzymolysis liquid obtained in step S1 is sterilized, then the first composite fermentation bacterial liquid is inoculated in the enzymolysis liquid for fermentation, sterilization, and filtration to obtain a fermentation liquid and a fermentation residue; wherein the sterilization temperature is 121 DEG C, the sterilization time is 20 min, the inoculation amount of the first composite fermentation bacterial liquid is 5% v / v, the total viable bacterial count of the first composite fermentation bacterial liquid is 2x10 9 CFU / mL, the first composite fermentation bacterial liquid comprises Phellinus baumii, Geotrichum candidum and Lactobacillus buchneri, the viable bacterial count ratio of the Phellinus baumii, the Geotrichum candidum and the Lactobacillus buchneri is 0.7:1:2, the fermentation temperature is 32 DEG C, and the fermentation time is 36 h;

[0057] S3, the fermentation residue obtained in step S2 is added to 3 times the mass of anhydrous ethanol for ultrasonic extraction, centrifugation, and collection of the supernatant; wherein the ultrasonic extraction temperature is 40 DEG C, the power is 200 W, and the time is 30 min;

[0058] S4, the fermentation liquid obtained in step S2 and the supernatant collected in step S3 are mixed, and freeze-drying is performed to obtain the Schisandra sphenanthera extract;

[0059] The preparation method of the greyhairy soybean seed extract comprises the following steps:

[0060] T1, the greyhairy soybean seed is crushed, 6 times the mass of water is added, the pH is adjusted to 5.5, the second composite enzyme is added for enzymolysis, the enzyme is inactivated, and it is cooled to room temperature to obtain an enzymolysis liquid; wherein the composite enzyme is papain and cellulase, the cellulase is added in an amount of 0.3% of the mass of the greyhairy soybean seed, the cellulase has an enzyme activity of 80000 U / g; the papain is added in an amount of 0.3% of the mass of the greyhairy soybean seed, the papain has an enzyme activity of 150000 U / g; the enzymolysis temperature is 55 DEG C, the time is 1.5 h, the inactivation temperature is 100 DEG C, and the inactivation time is 20 min;

[0061] T2, sterilizing the enzyme hydrolysate obtained in step T1, then inoculating a second compound fermentation bacterial liquid into the enzyme hydrolysate to perform fermentation, sterilizing, filtering, and obtaining a fermentation liquid and a fermentation residue; wherein the sterilization temperature is 121℃, the sterilization time is 20min, the inoculation amount of the second compound fermentation bacterial liquid is 4% v / v, the total viable bacterial count of the second compound fermentation bacterial liquid is 2x10 9 CFU / mL, the second compound fermentation bacterial liquid comprises G. fimbriatum and Y. lipolytica, the viable bacterial count ratio of the G. fimbriatum and Y. lipolytica is 1:1, the fermentation temperature is 32℃, and the fermentation time is 36h;

[0062] T3, adding the fermentation residue obtained in step T2 into 4 times the mass of anhydrous ethanol to perform ultrasonic extraction, centrifuging, and collecting the supernatant; wherein the ultrasonic extraction temperature is 40℃, the power is 200W, and the time is 30min;

[0063] T4, mixing the fermentation liquid obtained in step T2 and the supernatant collected in step T3, and freeze-drying to obtain the greyhairsed bean seed extract.

[0064] The mass ratio of the components of the sensitive skin care compositions with soothing and repairing effects of Examples 1-5 is shown in Table 1, and the total mass of each composition is equal.

[0065] The preparation method of the sensitive skin care compositions with soothing and repairing effects of Examples 1-5 comprises the following steps:

[0066] The Schisandra chinensis extract and the greyhairsed bean seed extract are mixed, and 4-tert-butylcyclohexanol is slowly added, and stirred at 45℃ until a uniform mixture is formed.

[0067] Table 1 Mass ratio of each component in each group of sensitive skin care compositions with soothing and repairing effects

[0068]

[0069] Example 6

[0070] Example 6 differs from Example 1 only in that in the preparation step S2 of the Schisandra chinensis extract of Example 6, the total viable bacterial count of the first compound fermentation bacterial liquid remains unchanged, and the viable bacterial count of the Phellinus igniarius, Geotrichum candidum, and Lactobacillus buchneri is 2:0.7:1.

[0071] Example 7

[0072] Example 7 differs from Example 1 only in that in the preparation step S2 of the Schisandra chinensis extract of Example 7, the total viable bacterial count of the first compound fermentation bacterial liquid remains unchanged, and the viable bacterial count of the Phellinus igniarius, Geotrichum candidum, and Lactobacillus buchneri is 0.7:2:1.

[0073] Comparative Example 1

[0074] Comparative Example 1 differs from Example 1 only in that Comparative Example 1 uses a commercially available Schisandra phenantnera extract instead of the Schisandra extract.

[0075] Comparative Example 2

[0076] Comparative Example 2 differs from Example 1 only in that the preparation method of the Schisandra extract in Comparative Example 2 (not fermented) includes the following steps:

[0077] The preparation method of the Schisandra extract includes the following steps:

[0078] S1, grinding Schisandra, adding 5 times the mass of water, adjusting the pH to 6, adding a first composite enzyme for enzymolysis, inactivating the enzyme, and cooling to room temperature to obtain an enzymolysis solution; wherein the first composite enzyme includes cellulase, pectinase, and papain, the cellulase is added in an amount of 0.5% of the mass of the Schisandra, the cellulase has an enzyme activity of 80000 U / g; the pectinase is added in an amount of 0.4% of the mass of the Schisandra, the pectinase has an enzyme activity of 90000 U / g; the papain is added in an amount of 0.2% of the mass of the Schisandra, the papain has an enzyme activity of 150000 U / g; the enzymolysis temperature is 43°C, and the time is 1.5 h, the inactivation temperature is 100°C, and the time is 20 min;

[0079] S2, sterilizing the enzymolysis solution obtained in step S1, then adding it to 3 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuging, and collecting the supernatant; wherein the ultrasonic extraction temperature is 40°C, the power is 200 W, and the time is 30 min;

[0080] S3, freeze-drying the supernatant collected in step S2 to obtain the Schisandra extract;

[0081] Comparative Example 3

[0082] Comparative Example 3 differs from Example 1 only in that in the preparation step S2 of the Schisandra extract in Comparative Example 3, the first composite fermentation bacteria solution does not add Phaeolus schweinitzii, and the total number of viable bacteria is supplemented with white mold and lactobacillus bulgaricus at a ratio of 1:2.

[0083] Comparative Example 4

[0084] Comparative Example 4 differs from Example 1 only in that in the preparation step S2 of the Schisandra extract in Comparative Example 4, the first composite fermentation bacteria solution does not add white mold, and the total number of viable bacteria is supplemented with Phaeolus schweinitzii and lactobacillus bulgaricus at a ratio of 0.7:2.

[0085] Comparative Example 5

[0086] The difference between Comparative Example 5 and Example 1 is only that in the preparation step S2 of the Schisandra chinensis extract of Comparative Example 5, the first compound fermentation bacteria liquid is not added with Lactobacillus buchneri, and the total viable bacteria number is supplemented with Phellinus igniarius and Geotrichum candidum with a viable bacteria number ratio of 0.7:1.

[0087] Comparative Example 6

[0088] The difference between Comparative Example 6 and Example 1 is only that Comparative Example 6 uses a commercially available Tephrosia purpurea seed extract to replace the Tephrosia purpurea seed extract.

[0089] Comparative Example 7

[0090] The difference between Comparative Example 7 and Example 1 is only that the preparation method of the Tephrosia purpurea seed extract of Comparative Example 7 comprises the following steps:

[0091] T1, grinding Tephrosia purpurea seeds, adding 6 times the mass of water, adjusting the pH to 5.5, adding a second compound enzyme for enzymolysis, inactivating the enzyme, and cooling to room temperature to obtain an enzymolysis liquid; wherein the compound enzyme is papain and cellulase, the cellulase is added in an amount of 0.3% of the mass of the Tephrosia purpurea seeds, and the cellulase has an enzyme activity of 80000 U / g; the papain is added in an amount of 0.3% of the mass of the Tephrosia purpurea seeds, and the papain has an enzyme activity of 150000 U / g; the enzymolysis temperature is 55°C, the time is 1.5h, the inactivation temperature of the enzyme is 100°C, and the time is 20min;

[0092] T2, sterilizing the enzymolysis liquid obtained in step T1, adding 4 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuging, and collecting the supernatant; wherein the ultrasonic extraction temperature is 40°C, the power is 200W, and the time is 30min;

[0093] T3, freeze-drying the supernatant collected in step T2 to obtain the Tephrosia purpurea seed extract.

[0094] Comparative Example 8

[0095] The difference between Comparative Example 8 and Example 1 is only that in the preparation step T2 of the Tephrosia purpurea seed extract of Comparative Example 8, the second compound fermentation bacteria liquid is not added with Aspergillus cristatus, and the total viable bacteria number is supplemented with Candida lipolytica.

[0096] Comparative Example 9

[0097] The difference between Comparative Example 9 and Example 1 is only that in the preparation step T2 of the Tephrosia purpurea seed extract of Comparative Example 9, the second compound fermentation bacteria liquid is not added with Candida lipolytica, and the total viable bacteria number is supplemented with Aspergillus cristatus.

[0098] Comparative Example 10

[0099] Comparative Example 10 differs from Example 1 only in that Comparative Example 10 uses a commercially available extract of Schisandra sphenanthera instead of the Schisandra chinensis extract, and a commercially available extract of Tephrosia purpurea seeds instead of the Tephrosia purpurea seed extract.

[0100] Test Example 1: Inhibition Test of Composition on Matrix Metalloproteinase (MMP-1) Expression

[0101] Studies have shown that epidermal keratinocytes irradiated by UV release cytokines, which indirectly promote the expression of matrix metalloproteinase (MMP-1) in dermal fibroblasts. MMP-1 is one of the key enzymes that leads to the degradation of skin barrier structures (especially collagen), and its overactivity directly weakens the supporting structure of the skin barrier and triggers a chain reaction, indirectly damaging the barrier function. Therefore, the skin barrier repair effect of the samples prepared above is evaluated by testing the inhibition rate of the samples on MMP-1.

[0102] The specific test method is as follows:

[0103] The compositions of Examples 1-7 and Comparative Examples 1-10 above were added to the cell DMEM culture medium and mixed to prepare a test sample solution with a mass concentration of 0.05%;

[0104] The cells (human keratinocytes HaCaT, purchased from the Kunming Cell Bank of the Chinese Academy of Sciences) were inoculated into a 12-well cell culture plate, each well containing 0.75 x 10 5 cells, and were starved for 24 hours in serum-free medium. The starved cells were rinsed with PBS and exposed to UVB light for 15 min (irradiation intensity: 85 μW / cm 2 , irradiation dose: 40 mJ / cm 2 ).

[0105] Grouping: The cell-containing medium above was divided into a sample group and a control group. The sample group was added with 2 mL of DMEM high-sugar culture solution containing a test sample solution prepared from the composition of Example or Comparative Example with a mass concentration of 0.05%; the control group was only added with 2 mL of DMEM high-sugar culture solution, and was cultured for 48 h.

[0106] MMP-1 content determination: according to the kit instructions (Shanghai Enzyme-linked Bio, item number: ml038199), the cells were lysed and the supernatant was collected. The absorbance at 450 nm was detected by ELISA method, and the concentration of MMP-1 (pg / mL) was calculated.

[0107] The skin barrier repair efficacy was evaluated by calculating the expression inhibition rate of MMP-1. The higher the inhibition rate, the better the barrier repair effect. The results are shown in Table 2; the calculation formula is as follows:

[0108] The expression inhibition rate of MMP-1 = (A-B) / A x 100%; in the formula, A is the expression amount of MMP-1 of the control group; B is the expression amount of MMP-1 of the experimental group; see Table 2 for specific data.

[0109] Test Example 2: Composition Inhibition Test on Sodium Dodecyl Sulfate Induced Zebrafish Surface Inflammation

[0110] The compositions of Examples 1-7 and Comparative Examples 1-10 above were added to the zebrafish culture solution and mixed to prepare a test sample solution with a mass concentration of 2%;

[0111] Group setting: blank control group (zebrafish embryo culture solution); model control group (zebrafish embryo culture solution + 60 μg / mL sodium dodecyl sulfate); experimental group (zebrafish embryo culture solution + 60 μg / mL sodium dodecyl sulfate + test sample solution).

[0112] Zebrafish culture solution: 2940 mg of anhydrous calcium chloride, 1233 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, 55 mg of potassium chloride, 1 g of methylene blue were dissolved in deionized water to make 10 L. All chemicals are analytical grade.

[0113] Test method: healthy zebrafish developed for 3 days after fertilization (screening criteria: normal morphology and development to blastula stage). At least 10 zebrafish were transferred to a 6-well plate for feeding, exposed according to the group setting, and cultured for 24 h. Fluorescence imaging: 10 zebrafish per group were placed under a fluorescence microscope to take fluorescence images of the zebrafish tail region.

[0114] Neutrophil quantification: Image processing software ImageJ was used to analyze and collect data, and the rectangular tool was used to accurately delineate the target area, and the number of zebrafish neutrophils was counted.

[0115] Table 2: Expression inhibition rate of MMP-1 and number of neutrophil migration on zebrafish surface of each composition

[0116] Group / Performance Inhibition rate of MMP-1 expression / % Number of neutrophil migration on the surface of zebra fish Example 1 54.1 13.5 Example 2 53.6 14.0 Example 3 54.8 13.0 Example 4 50.6 17.0 Example 5 52.4 15.5 Example 6 48.7 18.0 Example 7 49.1 18.5 Comparative Example 1 30.3 28.0 Comparative Example 2 33.6 25.5 Comparative Example 3 38.3 22.0 Comparative Example 4 39.7 21.5 Comparative Example 5 40.8 20.5 Comparative Example 6 32.2 27.0 Comparative Example 7 35.6 24.0 Comparative Example 8 42.2 19.5 Comparative Example 9 41.6 20.0 Comparative Example 10 26.8 33.0 Blank control group / 8.0 Model control group / 39.5

[0117] As can be seen from Table 2, in combination with the data of Examples 1 and 2-5, when the mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Millettia pinnata seed extract is 1: (0.02-0.04): (0.06-0.08), the soothing and anti-inflammatory effect of the composition is at a relatively optimal level.

[0118] Based on the data from Examples 1 and 6-7 in Table 2, the soothing and anti-inflammatory effects of the compositions in Examples 6-7 are lower than those in Example 1. This may be because the proportion of Geotrichum candida in the fermentation process of the Schisandra chinensis extract in Example 6 was too low, resulting in insufficient production of lignan derivatives (such as schisandrin A / B), which affected the soothing and anti-inflammatory effects of the composition. In Example 7, the proportion of Geotrichum candida in the fermentation process of the Schisandra chinensis extract was too high, leading to excessive degradation of lignans and a reduction in barrier repair and anti-inflammatory raw materials. This indicates that if the ratio of viable bacteria of Phellinus linteus, Geotrichum candida, and Lactobacillus brunelli is within the range of (0.5-1):1:(1.5-2.5) during the fermentation of the Schisandra chinensis extract, the synergistic effect of microorganisms during fermentation can be guaranteed, promoting the conversion and release of effective components in Schisandra chinensis.

[0119] Combination Figure 1 It can be seen that the number of neutrophils (black spots) in the tail area of ​​zebrafish treated with the composition of Example 1 was less than that in Comparative Example 1, indicating that the anti-inflammatory effect of the composition of Example 1 was better than that of Comparative Example 1, that is, the composition containing Schisandra chinensis extract after enzymatic hydrolysis-fermentation treatment has better anti-inflammatory efficacy.

[0120] Based on the data from Example 1 and Comparative Examples 1, 6, and 10 in Table 2, it can be seen that the composition of Comparative Example 10 exhibited the worst anti-inflammatory and soothing effects. This may be because the commercially available Schisandra chinensis extract and Edamame seed extract did not undergo enzymatic hydrolysis and fermentation, resulting in high levels of residual hydrophilic impurities (polysaccharides, proteins), which encapsulate the active ingredients, leading to low dissolution rates of lignans and isoflavones. The components exist in large molecular form, unable to penetrate the stratum corneum to reach the target site. Furthermore, the lack of repair-enhancing components such as fatty acids produced through enzymatic hydrolysis and fermentation affects the performance of the composition. This indicates that both Schisandra chinensis extract and Edamame seed extract require enzymatic hydrolysis and fermentation to achieve better anti-inflammatory and soothing effects in the composition.

[0121] From the data of Example 1 and Comparative Examples 2-5 in Table 2, it can be seen that the Schisandra chinensis extract of Comparative Example 2 only underwent enzymatic hydrolysis without fermentation treatment, and the Schisandra chinensis extract of Comparative Examples 3-5 lacked one kind of fungus in the fermentation process, and the anti-inflammatory and soothing effects of the compositions were lower than that of Example 1. This may be because the Schisandra chinensis extract of Comparative Example 2 did not undergo fermentation, and there were no high-activity lignan derivatives and short peptides, and only the original lignans played a basic role, so the anti-inflammatory and soothing performance of the composition was significantly reduced. In Comparative Examples 3-5, the lack of Phanerochaete chrysosporium resulted in insufficient repair raw materials for additional polysaccharide-assisted lipid synthesis. The lack of Geotrichum candidum led to the inability of lignan precursors to be converted into high-activity derivatives, affecting the anti-inflammatory and repair effects. The lack of Lactobacillus buchneri could not provide metabolic products such as lactic acid and short-chain fatty acids, affecting the pH regulation, skin metabolism promotion and self-emulsification of the system, thereby reducing the repair effect. It is shown that the fermentation of Schisandra chinensis enzymatic hydrolysate with Phanerochaete chrysosporium, Geotrichum candidum and Lactobacillus buchneri can synergistically improve the anti-inflammatory and soothing performance of the composition.

[0122] From the data of Example 1 and Comparative Examples 7-9 in Table 2, it can be seen that the anti-inflammatory and soothing repair of the compositions of Comparative Examples 7-9 were lower than that of Example 1. This may be because the Millettia pinnata seed extract of Comparative Example 7 lacked a fermentation process, and there were no high-activity anti-inflammatory derivatives (such as isoflavone aglycone) produced by fermentation, and only the inherent repair and anti-inflammatory ingredients in the raw material, and the macromolecular components were difficult to penetrate the zebrafish body surface, resulting in a decrease in the anti-inflammatory and soothing performance of the composition. In Comparative Examples 8-9, one kind of fermentation fungus was missing in the fermentation process of the Millettia pinnata seed extract. The lack of Aspergillus candidus led to the inability of isoflavone glycosides to be hydrolyzed into lipid-soluble aglycone, and the hydrophilic glycosides were difficult to penetrate the skin, so the repair effect was limited. The lack of Candida lipolytica resulted in no fatty acid to supplement lipids. It is shown that the Aspergillus candidus in the fermentation process of the Millettia pinnata seed extract hydrolyzes isoflavone glycosides into aglycone, and Candida lipolytica generates fatty acids, which play a natural emulsifying role, promote the dispersion of effective components, and synergistically strengthen barrier repair and anti-inflammatory.

[0123] Application Examples 1-7 and Comparative Application Examples 1-10

[0124] The compositions of Examples 1-7 and Comparative Examples 1-10 were added to the serum at a concentration of 2wt%, to obtain the serums of Application Examples 1-7 and Comparative Application Examples 1-10, and the formulations are shown in Table 3.

[0125] The preparation method of the serums of Application Examples 1-7 and Comparative Application Examples 1-10 includes the following steps:

[0126] S1, mixing the humectant, thickening agent and 1 / 2 amount of deionized water, homogenizing at 80°C to obtain a mixed solution;

[0127] S2. When the temperature of the mixture in S1 drops to 60°C, add the preservative. When the temperature of the system drops to 40°C, add the composition and the remaining deionized water, stir evenly, and finally add the pH adjuster to adjust the pH to 6 to obtain the essence.

[0128] Table 3. Serum formulations for Application Examples 1-7 and Comparative Application Examples 1-10

[0129]

[0130] Test Example 3: Human Patch Test of Each Group of Essences

[0131] Twenty volunteers were recruited, ten men and ten women, aged 20-50 years. A closed patch test method was used. To eliminate the influence of arm position, equal volumes (1-1.1 mL) of test samples (the serums of Application Examples 1-7 and Control Application Examples 1-10) were randomly placed in a specific patch applicator. The applicator was then applied to the volunteers' arms with hypoallergenic adhesive tape. Ten sets of test samples were applied to one arm, and seven sets to the other arm. The samples were gently pressed to ensure even application to the skin, and left on for 24 hours. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 hours, 24 hours, and 48 hours. The severity of adverse skin reactions is shown in Table 4 below.

[0132] Table 4. Adverse skin reaction grades

[0133] Score level Skin reaction 0 Negative reaction 1 Suspected reaction, only weak erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and possibly papule 3 Strong positive reaction (vesicle reaction): erythema, infiltration, edema, and papule; reaction may exceed the test area 4 Very strong positive reaction (fusion vesicle reaction): obvious erythema, severe infiltration, edema, and fusion vesicle; reaction exceeds the test area

[0134] After testing, such as Figure 2 As shown, after the serums of Application Examples 1-7 and Comparative Application Examples 1-10 were tested on human skin patches, two weak positive reactions were observed in Comparative Application Examples 1, 6 and 10, one suspicious reaction was observed in Comparative Application Examples 2 and 7, and the remaining application examples were all negative. The serums are safe and non-irritating to human skin.

[0135] Test Example 4: Stability Test of Each Group of Serums

[0136] Test procedure: Take 20 mL of the essence from Application Examples 1-7 and Comparative Application Examples 1-10 and place them in colorless and transparent glass bottles, then seal them. Place the sealed test samples in a standard light source D65 (6500K) at 55℃ for 4 weeks. Observe the layering of the test samples in the 4th week. The test results are shown in Table 5.

[0137] Table 5. Stability test results of each group of serums

[0138] Group Layering condition Application Example 1 No layering Application Example 2 No layering Application Example 3 No layering Application Example 4 No layering Application Example 5 No layering Application Example 6 Light layering, slight precipitation on top Application Example 7 Light layering, slight precipitation on top Comparative Application Example 1 Obvious layering, with floating oil and precipitation on top Comparative Application Example 2 Obvious layering, with clear separation between upper and lower layers Comparative Application Example 3 Light layering, with slight floating oil on top Comparative Application Example 4 Light layering, with slight floating oil on top Comparative Application Example 5 Light layering, slight precipitation on top Comparative Application Example 6 Obvious layering, with floating oil and precipitation on top Comparative Application Example 7 Obvious layering, with clear separation between upper and lower layers Comparative Application Example 8 Light layering, with slight floating oil on top Comparative Application Example 9 Light layering, with slight floating oil on top Comparative Application Example 10 Severe layering, complete separation between oil and water

[0139] From Table 5, in combination with the results of the layering of the serum in application examples 1 and 6-7, it can be seen that the storage stability of the serum in application examples 6-7 is lower than that in application example 1, which may be because the proportion of B. bosis in the fermentation process of the Schisandra chinensis extract in application example 6 is insufficient, resulting in a decrease in the generation of lipophilic components such as lactic acid and short-chain fatty acids, and the inability to fully coat the surface of 4-tert-butylcyclohexanol to form a hydrophilic protective film, and part of the 4-tert-butylcyclohexanol oil droplets aggregate and float up; and the proportion of G. candidum in the fermentation process of the Schisandra chinensis extract in application example 7 is too high, resulting in excessive degradation of lignans, resulting in a slight decrease in the proportion of fat-soluble components, and a decrease in the matching degree of the components with 4-tert-butylcyclohexanol. It is shown that if the viable bacterial count ratio of Phellinus baumii, G. candidum and B. bosis in the fermentation process of the Schisandra chinensis extract is within the range of (0.5-1):1:(1.5-2.5), the stability of the serum is at a relatively optimal level.

[0140] In combination with the results of the layering of the serum in application example 1 and comparative application examples 1, 6 and 10, it can be seen that the storage stability of the serum in comparative application examples 1, 6 and 10 is lower than that in application example 1, which may be because the commercially available Schisandra chinensis extract is used in comparative application example 1, and the commercially available gray hair soybean seed extract is used in comparative application example 6. Commercially available extracts are mostly simple alcohol extraction products, and there are more strong hydrophilic cellulose, pectin and macromolecular protein residues. Moreover, the strong hydrophilic components and the fat-soluble 4-tert-butylcyclohexanol form a polarity conflict, and the active components of Schisandra chinensis and gray hair soybean seeds are macromolecules (lignan precursors, isoflavone glycosides), without small molecule modification, and the proportion of fat-soluble components is low, and the components do not match with 4-tert-butylcyclohexanol. Moreover, there is a lack of lactic acid, fatty acid and other natural stabilizing substances produced by enzymatic hydrolysis and fermentation. Due to the lack of emulsifying and solubilizing components in the serum system, 4-tert-butylcyclohexanol and the two extracts are separated into oil and water. It is shown that the key factor for improving the stability of the serum is to complex the Schisandra chinensis extract and the gray hair soybean seed extract with 4-tert-butylcyclohexanol after enzymatic hydrolysis and fermentation.

[0141] It can be known from the layering results of the serum of application example 1 and comparative application examples 2-5 that the storage stability of the serum of comparative application examples 2-5 is lower than that of application example 1, which may be because the Schisandra chinensis extract in comparative application example 2 is not subjected to fermentation treatment, and although the enzymatic hydrolysis degrades part of the macromolecular impurities, the active ingredients are not converted into liposoluble small molecules (such as lignan derivatives) by fermentation, and the extract is still hydrophilic, which conflicts with the polarity of 4-tert-butylcyclohexanol and is not fundamentally solved. In comparative application examples 3-5, the Schisandra chinensis extract lacks a certain fermentation fungus in the fermentation process, which may lead to: lacking of Phellinus igniarius, the additional polysaccharide cannot assist in stabilizing the system, and the dispersibility is reduced; lacking of Geotrichum candidum, the lignan precursors cannot be converted into highly active liposoluble derivatives, and the proportion of liposoluble components is insufficient, and the matching degree with 4-tert-butylcyclohexanol is reduced; lacking of Lactobacillus buchneri, no components with amphiphilic properties such as lactic acid and short-chain fatty acids are generated, and the oil droplets of 4-tert-butylcyclohexanol are easily aggregated. This shows that the Schisandra chinensis extract subjected to the synergistic fermentation of Geotrichum candidum, Lactobacillus buchneri and Phellinus igniarius can improve the compatibility of the composition with 4-tert-butylcyclohexanol.

[0142] It can be known from the layering results of the serum of application example 1 and comparative application examples 7-9 that the storage stability of the serum of comparative application examples 7-9 is lower than that of application example 1, which may be because the grey hair bean seed extract in comparative application example 7 is not subjected to fermentation treatment, and the impurities are still relatively high, and the polarity conflict with 4-tert-butylcyclohexanol is not completely solved. In comparative application examples 8-9, the fermentation process of the grey hair bean seed extract lacks a certain fermentation fungus, and lacking of Aspergillus corallinus, β-glucosidase is not produced, isoflavone glycosides cannot be hydrolyzed into liposoluble aglycone, the proportion of hydrophilic glycosides increases, and the polarity conflict with 4-tert-butylcyclohexanol still exists; and lacking of Candida lipolytica, no fatty acids are generated in the system, and the dispersion and mixing of oil and water are not promoted, and the dispersion stability of the serum is reduced. Therefore, Aspergillus corallinus hydrolyzes isoflavone glycosides into liposoluble aglycone to form a mixed oil phase with 4-tert-butylcyclohexanol, and Candida lipolytica produces fatty acids to play a natural emulsifying role, and the grey hair bean seed extract obtained by the synergistic fermentation of the two can further improve the stability of the serum.

[0143] It can be known from the layering results of the serum of application example 1 and comparative application examples 7-9 that the storage stability of the serum of comparative application examples 7-9 is lower than that of application example 1, which may be because the grey hair bean seed extract in comparative application example 7 is not subjected to fermentation treatment, and the impurities are still relatively high, and the polarity conflict with 4-tert-butylcyclohexanol is not completely solved. In comparative application examples 8-9, the fermentation process of the grey hair bean seed extract lacks a certain fermentation fungus, and lacking of Aspergillus corallinus, β-glucosidase is not produced, isoflavone glycosides cannot be hydrolyzed into liposoluble aglycone, the proportion of hydrophilic glycosides increases, and the polarity conflict with 4-tert-butylcyclohexanol still exists; and lacking of Candida lipolytica, no fatty acids are generated in the system, and the dispersion and mixing of oil and water are not promoted, and the dispersion stability of the serum is reduced. Therefore, Aspergillus corallinus hydrolyzes isoflavone glycosides into liposoluble aglycone to form a mixed oil phase with 4-tert-butylcyclohexanol, and Candida lipolytica produces fatty acids to play a natural emulsifying role, and the grey hair bean seed extract obtained by the synergistic fermentation of the two can further improve the stability of the serum. Figure 3

[0144] ​In summary, the present application actively modifies the Schisandra chinensis extract and the Millettia pinnata seed extract by enzymatic hydrolysis and fermentation, releases active ingredients by enzymatic hydrolysis and removes hydrophilic impurities, realizes small molecule of the ingredients, enhances liposolubility, and generates natural emulsifying ingredients at the same time, so as to realize the essential adaptation with 4-tert-butylcyclohexanol from the root, and therefore, it is not necessary to add emulsifiers, oils, polyols and other ingredients in the composition to improve the compatibility of the Schisandra chinensis extract, the Millettia pinnata seed extract and 4-tert-butylcyclohexanol, and the composition can be directly adapted to various dosage forms such as water-based, alcohol-free and refreshing types (such as toner and essence), and the formula texture can be adjusted according to the sensitive skin types (dry and oily sensitive skin).

[0145] Finally, it should be explained that the above examples are only used to illustrate the technical solutions of the present application, but not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present application can be modified or replaced equivalently without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A skincare composition for sensitive skin with soothing and repairing effects, characterized in that, It is composed of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract, wherein the mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract is 1:(0.01-0.1):(0.01-0.1). The preparation method of the Schisandra chinensis extract includes the following steps: S1. Crush Schisandra chinensis, add 5-6 times its weight of water, adjust the pH to 5.5-6.5, add the first complex enzyme for enzymatic hydrolysis, inactivate the enzyme, cool to room temperature, and obtain the enzymatic hydrolysate; wherein, the first complex enzyme includes cellulase, pectinase and papain. S2. Sterilize the enzymatic hydrolysate obtained in step S1, then inoculate the enzymatic hydrolysate with the first compound fermentation liquid for fermentation, sterilize, filter, and obtain fermentation liquid and fermentation residue; wherein, the first compound fermentation liquid includes Phellinus linteus, Geotrichum candida, and Lactobacillus brunelli, and the viable cell ratio of Phellinus linteus, Geotrichum candida, and Lactobacillus brunelli is (0.5-1):1:(1.5-2.5); S3. Add the fermentation residue obtained in step S2 to 3-4 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant. S4. Mix the fermentation broth obtained in step S2 and the supernatant collected in step S3, and freeze-dry to obtain the Schisandra chinensis extract. The preparation method of the grey soybean seed extract includes the following steps: T1. Crush the soybean seeds, add 5-6 times the weight of water, adjust the pH to 5.5-6.5, add the second complex enzyme for enzymatic hydrolysis, inactivate the enzyme, cool to room temperature, and obtain the enzymatic hydrolysate; wherein, the second complex enzyme is papain and cellulase. T2. Sterilize the enzymatic hydrolysate obtained in step T1, then inoculate the enzymatic hydrolysate with the second compound fermentation culture for fermentation, sterilize, filter, and obtain fermentation broth and fermentation residue; the second compound fermentation culture includes Aspergillus cristatus and Candida lipolyticis. T3. Add the fermentation residue obtained in step T2 to 3-4 times its weight of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant. T4. Mix the fermentation broth obtained in step T2 and the supernatant collected in step T3, and freeze-dry to obtain the gray soybean seed extract.

2. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, The mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract is 1:(0.02-0.04):(0.06-0.08).

3. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step S1 of preparing the Schisandra chinensis extract, the amount of cellulase added is 0.3-0.6% of the mass of Schisandra chinensis, and the enzyme activity of cellulase is 50,000-100,000 U / g; the amount of pectinase added is 0.2-0.5% of the mass of Schisandra chinensis, and the enzyme activity of pectinase is 50,000-100,000 U / g; the amount of papain added is 0.1-0.5% of the mass of Schisandra chinensis, and the enzyme activity of papain is 100,000-200,000 U / g; the enzymatic hydrolysis temperature is 40-45℃ and the time is 1-2 hours.

4. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step S2 of preparing the Schisandra chinensis extract, the inoculum size of the first compound fermentation broth is 2-7% v / v, and the total viable count of the first compound fermentation broth is (1-3) × 10⁻⁶. 9 The fermentation concentration was CFU / mL, with a fermentation temperature of 30-35℃ and a fermentation time of 24-72h.

5. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step T1 of preparing the grey soybean seed extract, the amount of cellulase added is 0.2-0.5% of the mass of grey soybean seeds, and the enzyme activity of cellulase is 50,000-100,000 U / g; the amount of papain added is 0.2-0.5% of the mass of grey soybean seeds, and the enzyme activity of papain is 100,000-200,000 U / g; the enzymatic hydrolysis temperature is 50-60℃ and the time is 1-2 h.

6. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step T2 of the preparation of the soybean seed extract, the inoculation amount of the second compound fermentation broth is 3-5% v / v, and the total viable count of the second compound fermentation broth is (1-3) × 10⁻⁶. 9 The viable cell count ratio of Aspergillus cristatus and Candida lipolyticis was (0.5-1.5):1, and the fermentation temperature was 30-35℃ for 24-72h.

7. The use of a skin care composition for sensitive skin with soothing and repairing effects as described in any one of claims 1-6 in the preparation of skin cosmetics.

8. A skin cosmetic product, characterized in that, The skin cosmetics include the sensitive skin skin care composition with soothing and repairing effects as described in any one of claims 1-6.

9. The skin cosmetic product as described in claim 8, characterized in that, The skin cosmetic product comprises the following components in weight percentage: 0.1-5% of the sensitive skin skin care composition with soothing and repairing effects as described in any one of claims 1-6, 2-40% of the cosmetic base, and the balance being water.

10. The skin cosmetic product as described in claim 9, characterized in that, The skin cosmetics include any one of the following: toner, cream, face mask, serum, facial oil, and spray.

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