A bird's nest compound product with improved female body health effect and a preparation method and application thereof

Abstract

The application discloses a bird's nest compound product with the effect of improving female body health and a preparation method and application thereof. The preparation method comprises the following steps: mixing red ginseng, Gastrodia elata, ophiopogon, donkey-hide gelatin, eucommia and ganoderma lucidum with a solvent to form a mixed slurry; treating and extracting the mixed slurry by applying an electric field to prepare a compound liquid; and mixing the compound liquid with bird's nest to prepare the bird's nest compound product with the effect of improving female body health. The bird's nest is matched with the medicine-food homologous material, and on the basis of the theory of "suo zhuo jun chen", the bird's nest compound product capable of obviously improving menopausal syndrome is prepared, the hormone level is effectively adjusted, symptoms such as hot flashes, night sweats, sleep quality improvement, ovary and uterus repair promotion and the like are relieved, and the product is mild in action, high in safety, good in flavor and color.

Description

Technical Field

[0001] This application relates to the field of functional composition technology, and in particular to a bird's nest compound product with the effect of improving women's health, its preparation method and application. Background Technology

[0002] Bird's nest, a traditional and rare nourishing food, is rich in sialic acid, epidermal growth factor (EGF), collagen, and various amino acids and minerals. It is believed to have effects such as nourishing yin and moisturizing dryness, replenishing qi and strengthening the body, and improving overall health. Modern medical and food-related research indicates that bird's nest possesses antioxidant, immune-regulating, and anti-fatigue physiological functions. In recent years, products combining bird's nest with medicinal and edible ingredients have gained increasing attention, aiming to enhance the functional activity of products containing bird's nest through ingredient compatibility. However, the application of this technology in women's health, such as alleviating menopausal syndrome, has not yet been systematically researched and commercialized. Summary of the Invention

[0003] Therefore, it is necessary to provide a compound product containing bird's nest and medicinal and edible ingredients, as well as its preparation method, which has excellent effects in relieving menopausal syndrome in women.

[0004] In a first aspect, this application provides a method for preparing a bird's nest compound product with effects on improving women's health, comprising the following steps:

[0005] Red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides, and ganoderma lucidum are mixed with a solvent to form a mixed slurry;

[0006] The mixed slurry is treated and extracted by applying an electric field to prepare a compound solution;

[0007] The compound solution is mixed with bird's nest to prepare a bird's nest compound product that improves women's health.

[0008] In some embodiments, during the preparation process, the raw materials of the bird's nest compound product, by weight, include 3-10 parts bird's nest, 6-12 parts red ginseng, 6-12 parts gastrodia elata, 9-15 parts ophiopogon japonicus, 6-12 parts donkey-hide gelatin, 12-20 parts eucommia ulmoides, and 1-10 parts ganoderma lucidum.

[0009] In some embodiments, the solvent includes water; and / or, the total mass ratio of the red ginseng, the gastrodia elata, the ophiopogon japonicus, the donkey-hide gelatin, the eucommia ulmoides and the ganoderma lucidum to the solvent is 1:(30-50).

[0010] In some embodiments, the strength of the electric field is 4kV / m-7kV / m; and / or, the treatment time of the electric field is 30min-40min; and / or, the extraction solvent includes one or more of ethanol and water; and / or, the extraction temperature is 65℃-75℃; and / or, the extraction time is 40min-50min.

[0011] In some embodiments, the extraction step is followed by a decolorization treatment of the extract obtained by pulsed light.

[0012] In some embodiments, the light source of the pulsed light is ultraviolet light; and / or, the energy of the pulsed light is 0.1 J / cm². 2 -0.5J / cm 2 ; and / or, the frequency of the pulsed light is 50Hz-70Hz.

[0013] In some embodiments, the decolorization process is followed by a taste-correcting treatment of the decolorized liquid using plasma-activated water.

[0014] In some embodiments, the oxidation-reduction potential of the plasma-activated water is >800mV; and / or the temperature of the plasma-activated water is 40℃-60℃; and / or the flavoring treatment time is 10min-30min.

[0015] Secondly, this application also provides a bird's nest compound product that has the effect of improving women's health, which is prepared by the preparation method of the bird's nest compound product with the effect of improving women's health provided in the first aspect.

[0016] Thirdly, this application also provides the application of bird's nest compound products in the preparation of preparations for preventing or improving menopausal symptoms in women. The bird's nest compound products include bird's nest compound products prepared by the preparation method provided in the first aspect that have the effect of improving women's health, or bird's nest compound products provided in the second aspect that have the effect of improving women's health.

[0017] In some embodiments, the symptoms of female menopause include one or more of the following: uterine and ovarian insufficiency, hot flashes and night sweats, and decreased sleep quality.

[0018] Compared with traditional technologies, the beneficial effects of the technical solution in this application include:

[0019] This application provides a method for preparing a compound bird's nest product with effects on improving women's health. The method involves applying an electric field to treat and extract medicinal and edible raw materials such as red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides, and ganoderma lucidum to obtain a compound solution, which is then combined with bird's nest to form a compound bird's nest product with effects on improving women's health. This application utilizes electric field enrichment technology to pretreat the medicinal and edible raw materials, synergistically improving the extraction efficiency of these materials. This provides conditions for better dissolution of specific active ingredients while reducing the degradation of heat-sensitive active substances, fully preserving the bioactivity of specific active substances in the medicinal and edible materials. The combined extraction process specifically enriches active substances, thereby enhancing the efficacy and activity of the product. This application utilizes a "coordinating and supporting" approach to combine medicinal and edible materials, combining bird's nest with medicinal and edible materials obtained through specific processing methods to create a bird's nest compound product that can significantly improve menopausal syndrome, enhance uterine and ovarian function, effectively regulate hormone levels, improve sleep quality, promote ovarian and uterine repair, and relieve symptoms such as hot flashes and night sweats. It is also gentle, safe, and has a good flavor and color. Attached Figure Description

[0020] Figure 1 The images show actual photos of the bird's nest compound products prepared in Example 1 (left) and Comparative Example 2 (right).

[0021] Figure 2 The changes in the estrous cycle of mice in the blank group and the model group are shown (n=10), with a scale bar of 100μm for both groups.

[0022] Figure 3 This figure shows the effect of different formulations of bird's nest compound products on serum E2 levels in mice; the figure is compared with the control group. <0.001, <0.0001; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0023] Figure 4 This figure shows the effects of different formulations of bird's nest compound products on serum LH levels in mice; the figure is compared to the control group. <0.001, <0.0001; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0024] Figure 5 This figure shows the effects of different formulations of bird's nest compound products on serum FSH levels in mice; the figure is compared to the control group. <0.001, <0.0001; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0025] Figure 6 This figure shows the effect of different dosage groups of bird's nest compound products on serum E2 levels in mice; the figure is compared with the blank group. <0.001, <0.0001; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0026] Figure 7 This figure shows the effect of different dosage groups of bird's nest compound products on serum LH levels in mice; the figure shows the effect of different dosage groups on the control group. <0.001, <0.0001; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0027] Figure 8 This figure shows the effect of different dosage groups of bird's nest compound products on serum FSH levels in mice; the figure is compared with the blank group. <0.001, <0.0001; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0028] Figure 9 This study demonstrates the effects of different dosage groups of the bird's nest compound product on the sleep latency of mice; vs. the control group. <0.05; vs. model group, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001.

[0029] Figure 10 The effects of different doses of bird's nest compound products on the uterine morphology of mice are shown (n=3); in the figure, A-blank group, B-model group, C-low dose group, D-medium dose group and E-high dose group, the scale bar is 1 mm.

[0030] Figure 11 The effect of different doses of the bird's nest compound product on the ovarian morphology of mice is shown (n=3); in the figure, A-blank group, B-model group, C-low-dose group, D-medium-dose group, and E-high-dose group. The numbers in the figure are labeled as follows: 1-primordial follicle; 2-primary follicle; 3-secondary follicle; 4-mature follicle.

[0031] Figure 12 The effect of different dose groups of bird's nest compound products on sweat secretion in mice is shown (n=3); in the figure, A-blank group, B-model group, C-low dose group, D-medium dose group and E-high dose group. Detailed Implementation

[0032] To facilitate understanding of the present invention, a more complete description will be given below with reference to the accompanying drawings. Preferred embodiments of the invention are shown in the drawings. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the invention.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0034] As used herein, "optional," "optional," and "optional" refer to either "with" or "without" parallel options. If multiple "optional" entries appear in a technical solution, each "optional" entry is independent unless otherwise specified and there are no contradictions or mutual constraints. The term "and / or" as used herein includes any and all combinations of one or more related listed items. Unless otherwise specified, "multiple," "multiple," etc., as used herein refer to a quantity greater than 2 or equal to 2; for example, "one or more" indicates one, two, or more than two. In open-ended technical features or solutions described herein using words such as "containing," "including," and "comprising," unless otherwise specified, additional members beyond the listed members are not excluded. This can be considered as providing both a closed-ended feature or solution consisting of the listed members and an open-ended feature or solution that includes additional members beyond the listed members.

[0035] In this application, the terms "first aspect," "second aspect," "third aspect," "fourth aspect," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or quantity, nor should they be construed as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first," "second," "third," "fourth," etc., serve only as a non-exhaustive enumeration and should be understood not to constitute a closed limitation on quantity.

[0036] Unless otherwise specified, all embodiments and optional embodiments of this application can be combined to form new technical solutions.

[0037] The term "Maillard reaction," also known as non-enzymatic browning, refers to the Maillard reaction between amino acids (derived from proteins) and reducing sugars in food. It is also called non-enzymatic browning and is a type of non-enzymatic browning widely present in the food industry. It is a series of complex chemical reactions that occur between carbonyl compounds (reducing sugars) and amino compounds (amino acids and proteins) under heating conditions.

[0038] The term "Plasma Activated Water" (PAW) refers to an aqueous solution containing reactive oxygen species (ROS) and reactive nitrogen species (RNS) formed after water or other liquids have been treated with low-temperature plasma.

[0039] Menopause is a crucial physiological period for women transitioning from reproductive age to old age. With the gradual decline in ovarian function and estrogen levels, some women experience a cluster of clinical symptoms primarily characterized by autonomic nervous system dysfunction, collectively known as menopausal syndrome. Typical symptoms include menstrual cycle irregularities, hot flashes and night sweats (especially in the head, neck, and chest), insomnia and vivid dreams, mood swings (such as irritability, anxiety, and paranoia), memory loss, palpitations, chest tightness, lower back pain, and sensitivity to heat and cold in the extremities. These symptoms significantly impact patients' quality of life and overall well-being.

[0040] Currently, interventions for menopausal syndrome mainly include hormone replacement therapy (HRT), psychological and behavioral interventions, dietary therapy, and drug treatment. However, HRT has potential side effects such as increased risk of thrombosis and breast cancer, and some patients have low adherence rates; psychological and behavioral interventions are time-consuming, easily affected by external factors, and have limited effectiveness. Therefore, dietary therapy and drug treatment remain the primary options.

[0041] Modern medical and food studies have shown that bird's nest, as a traditional tonic food, is rich in nutrients, nourishing yin and moisturizing dryness, replenishing qi and strengthening the middle jiao (spleen and stomach), and has antioxidant, immune-regulating, anti-fatigue, and body-improving effects. However, its efficacy when used alone is limited. In recent years, research on combining bird's nest with medicinal and food homologous formulas has gradually attracted attention, aiming to enhance its efficacy through functional synergy. However, its application in regulating endocrine function and alleviating menopausal syndrome has not yet been systematically studied or commercialized.

[0042] Based on this, this application aims to propose a bird's nest product for improving menopausal symptoms in women. By scientifically combining bird's nest with medicinal and edible ingredients, it enhances the regulatory effect on the neuroendocrine system, providing a safe and effective solution for menopausal women.

[0043] In the first aspect, this application provides a bird's nest compound product with the effect of improving women's health. By weight, the raw materials of the bird's nest compound product include 3-10 parts bird's nest, 6-12 parts red ginseng, 6-12 parts gastrodia elata, 9-15 parts ophiopogon japonicus, 6-12 parts donkey-hide gelatin, 12-20 parts eucommia ulmoides and 1-10 parts lingzhi mushroom.

[0044] Guided by the traditional Chinese medicine theories of "nourishing yin and moistening dryness, calming the mind and soothing the nerves, and balancing yin and yang," and based on the theories of "medicine and food sharing the same origin" and "group health care," this formula is scientifically formulated through data mining and modern pharmacological research. Bird's nest, red ginseng, Ophiopogon japonicus, and donkey-hide gelatin are the principal ingredients. Bird's nest, as the core principal ingredient, governs the entire formula, highlighting its core effects and traditional advantages of nourishing yin and moistening the lungs, and tonifying the spleen and replenishing qi. Red ginseng greatly replenishes vital energy and enhances the body's resistance; Ophiopogon japonicus nourishes yin and clears the heart, and regulates endocrine function; donkey-hide gelatin nourishes blood and replenishes essence, replenishes blood and nourishes yin, and nourishes the ovaries, working together to replenish qi and nourish yin, replenish blood and soothe the mind. Eucommia ulmoides leaves are used as the assistant ingredient, tonifying the liver and kidneys, strengthening muscles and bones, and enhancing immunity, thus strengthening the liver and kidney tonifying effects of the principal ingredients. Gastrodia elata and Ganoderma lucidum are used as adjuvant ingredients. Gastrodia elata calms the liver and extinguishes wind, relieves dizziness, and regulates nerve function; Ganoderma lucidum replenishes qi and soothes the mind, calms the will, and strengthens the body's resistance. These ingredients assist the principal and assistant ingredients in improving symptoms such as irritability, insomnia, hot flashes, and night sweats. When combined with bird's nest, these herbs comprehensively enhance the body's adaptogenic effects and immune-endocrine regulatory functions. They work synergistically with other herbs to treat menopausal symptoms such as hot flashes, night sweats, insomnia, anxiety, lower back and knee pain, and mood swings caused by declining ovarian function and estrogen levels. Targeting the core symptoms of yin deficiency and excessive fire, and restlessness in women during menopause, they work together to regulate endocrine function and improve physical and emotional symptoms.

[0045] This application describes a compound bird's nest product formulated by combining bird's nest, red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides, and ganoderma lucidum in a specific ratio. The product has excellent efficacy and activity, and can significantly alleviate menopausal symptoms, especially for women's health. It can also improve uterine and ovarian function, and has a significant repairing effect on damage to the uterus and ovaries.

[0046] Furthermore, in selecting ingredients that are both medicinal and edible, this application considers two factors: firstly, the absence of significant acidity or astringency in the food extract; and secondly, the inclusion of ingredients with minimal impact on the expansion and rehydration of bird's nest. This aims to reduce the significant shrinkage of the bird's nest during processing and ensure the stability of its texture and shape. For example, if the selected ingredients significantly impact the expansion and rehydration of the bird's nest, the bird's nest will shrink after the extract is combined with the bird's nest for sterilization, resulting in a brittle texture and affecting both its shape and taste.

[0047] It is understood that bird's nest, red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides, and ganoderma lucidum include the corresponding raw materials and / or extracts. For example, bird's nest includes, but is not limited to, its source, type, and quality, and can be dried bird's nest. Red ginseng includes, but is not limited to, red ginseng medicinal materials and / or red ginseng extracts, and the form of red ginseng medicinal materials includes, but is not limited to, red ginseng slices and / or red ginseng powder. Gastrodia elata includes, but is not limited to, gastrodia elata medicinal materials and / or gastrodia elata extracts, and the gastrodia elata medicinal materials are not limited to its source and type, including, but not limited to, one or more of green gastrodia elata, black gastrodia elata, yellow gastrodia elata, pine gastrodia elata, red gastrodia elata, golden thread gastrodia elata, black gastrodia elata, and apocynum venetum. Ophiopogon japonicus includes, but is not limited to, ophiopogon japonicus medicinal materials and / or ophiopogon japonicus extracts, and the ophiopogon japonicus medicinal materials are not limited to its source and type, including, but not limited to, one or more of Zhejiang ophiopogon japonicus, Sichuan ophiopogon japonicus, large ophiopogon japonicus, and bamboo leaf ophiopogon japonicus. Eucommia ulmoides includes, but is not limited to, eucommia ulmoides medicinal materials and / or eucommia ulmoides extracts, and eucommia ulmoides medicinal materials and eucommia ulmoides extracts include, but are not limited to, eucommia ulmoides leaves or eu For example, Ganoderma lucidum includes, but is not limited to, Ganoderma lucidum medicinal materials and / or Ganoderma lucidum extracts. Ganoderma lucidum medicinal materials include, but are not limited to, Ganoderma lucidum body and Ganoderma lucidum spores. Ganoderma lucidum body includes, but is not limited to, its source and category, such as one or more of Ganoderma lucidum, Ganoderma lucidum var. rubrum ... and Ganoderma lucidum var. rubrum.

[0048] In some preferred embodiments, the raw materials of the bird's nest compound product include 3-5 parts bird's nest, 6-8 parts red ginseng, 6-8 parts gastrodia elata, 9-12 parts ophiopogon japonicus, 6-8 parts donkey-hide gelatin, 12-15 parts eucommia ulmoides, and 1-3 parts ganoderma lucidum.

[0049] In some embodiments, the raw materials of the bird's nest compound product also include excipients.

[0050] In some embodiments, the excipients include food excipients or pharmaceutical excipients. As a non-limiting example, the excipients include rock sugar. Further, the amount of rock sugar added to the bird's nest compound product is 5-15 parts by weight. Even further, the amount of rock sugar added to the bird's nest compound product is 5-8 parts by weight.

[0051] In the process of combining bird's nest with medicinal and edible ingredients, processing technology is also a key technical challenge affecting its efficacy and activity.

[0052] On the one hand, when bird's nest is processed in combination with ingredients that are both food and medicine, the protein in bird's nest and the active ingredients such as polysaccharides and polyphenols in the food and medicine are prone to Maillard reaction under heat treatment conditions, which leads to darkening of product color, deterioration of flavor and degradation of functional components.

[0053] On the other hand, during the processing, the active ingredients of substances that are both food and medicine are not fully extracted, the material utilization rate is extremely low, and the efficacy and activity of the product after formulation are greatly reduced. In addition, traditional separation and purification technologies have problems such as separation difficulties, low separation accuracy, loss of active ingredients due to adsorption, low production efficiency and high cost, making it difficult to achieve industrial production.

[0054] In response to the aforementioned technical problems, secondly, this application also provides a method for preparing a bird's nest compound product with effects on improving women's health, comprising the following steps:

[0055] S10. Mix red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides and ganoderma lucidum with solvent to form a mixed slurry.

[0056] S20. Apply an electric field to the mixed slurry for treatment and extraction to prepare a compound solution.

[0057] S30. The compound solution is mixed with bird's nest to prepare a bird's nest compound product that improves women's health.

[0058] This application employs electric field enrichment technology to pretreat the mixed slurry prepared from medicinal and edible materials. By using an electric field, ion cyclic enrichment and slight alteration of the permeability of the material's cell walls are achieved, thereby reducing dissolution resistance and synergistically improving the extraction efficiency of medicinal and edible materials. This provides conditions for better dissolution of specific active ingredients.

[0059] The formula in this application utilizes the principles of "nourishing yin and reducing fire, invigorating qi and calming the mind, soothing the liver and suppressing yang, and nourishing blood and tonifying the kidneys" to comprehensively regulate the core pathogenesis of yin-yang imbalance caused by "kidney essence deficiency and depletion of Tian Gui" during female menopause. Bird's nest, being neutral in nature and sweet in taste, enters the lung, stomach, and kidney meridians. Its functions of nourishing yin and moistening dryness, tonifying the lungs and kidneys, form the important foundation for nourishing yin and blood in the formula. It alleviates dry skin and deficiency heat caused by yin deficiency by moistening the lungs, and works synergistically with Ophiopogon japonicus and donkey-hide gelatin to treat the root cause of yin deficiency. Red ginseng is added to invigorate qi and consolidate essence, Gastrodia elata to soothe the liver and suppress yang, Ganoderma lucidum to nourish the heart and calm the mind, and Eucommia ulmoides leaves to tonify the liver and kidneys. The combination of these herbs allows the entire formula to nourish yin and blood, clear deficiency fire to improve hot flashes and night sweats, invigorate qi and calm the mind, soothe the liver and suppress yang to stabilize nerves, regulate emotions and sleep, aiming to alleviate menopausal symptoms by regulating the overall yin-yang balance of the body. In summary, the bird's nest in this application works synergistically with other food and medicine ingredients such as red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides, and ganoderma lucidum to alleviate menopausal symptoms and improve the user's quality of life and physical and mental health.

[0060] In some embodiments, during the preparation process, the raw materials of the bird's nest compound product with the effect of improving women's health include, by weight, 3-10 parts bird's nest, 6-12 parts red ginseng, 6-12 parts gastrodia elata, 9-15 parts ophiopogon japonicus, 6-12 parts donkey-hide gelatin, 12-20 parts eucommia ulmoides and 1-10 parts ganoderma lucidum.

[0061] In some embodiments, the excipients include food excipients or pharmaceutical excipients. As a non-limiting example, the excipients include rock sugar. Further, the amount of rock sugar added in the compound product is 5-15 parts by weight.

[0062] In some embodiments, the solvent includes water.

[0063] In some embodiments, the total mass ratio of the red ginseng, the gastrodia elata, the ophiopogon japonicus, the donkey-hide gelatin, the eucommia ulmoides and the ganoderma lucidum to the solvent is 1:(30-50), including but not limited to 1:30, 1:35, 1:40, 1:45, 1:50 or any of the foregoing ranges and values ​​within those ranges.

[0064] In some embodiments, the pH of the mixed slurry is 6-7, including but not limited to 6, 6.2, 6.4, 6.6, 6.8, 7 or any combination thereof and values ​​within that range.

[0065] In some embodiments, the electric field strength is 4kV / m-7kV / m, including but not limited to 4kV / m, 5kV / m, 6kV / m, 7kV / m, or any combination thereof, and values ​​within that range. Within this electric field strength range, the dissolution effect of specific active ingredients is more favorable.

[0066] In some embodiments, the electric field treatment time is 30 min to 40 min, including but not limited to 30 min, 32 min, 34 min, 36 min, 38 min, 40 min or any of the foregoing ranges and values ​​within that range.

[0067] In the development of the technical solution of this application, conventional dissolution or extraction processes were initially used to treat the specific formulation compound product described in this application. The effects of multiple process parameters, such as temperature, time, solvent system, and treatment method, on the stability of the active ingredients in the compound product were systematically investigated. The results showed that conventional dissolution or extraction processes severely damaged the heat-sensitive active ingredients (e.g., polyphenols, flavonoids, polysaccharides, saponins, etc.) in the specific formulation described in this application, making it difficult for the final product to achieve the expected menopausal conditioning effects in women. To solve this technical problem, the applicant creatively adopted electric field enrichment technology and conducted numerous systematic experiments around this technology, thoroughly investigating the effects of multiple process parameters, such as electric field morphology or mode, electric field strength, and treatment time, on the stability of the active ingredients in the compound product. After repeated optimization and verification, a mild and efficient dissolution process was finally determined. This process significantly reduces the degradation of heat-sensitive active substances while fully preserving the bioactivity of each component, selectively enriching active substances, and synergistically enhancing the overall conditioning effect on menopausal-related symptoms in women, achieving the expected results.

[0068] This application achieves ion cyclic enrichment and slightly alters the permeability of material cell walls by applying a low-voltage electric field to a mixed slurry, reducing dissolution resistance and promoting the dissolution of active ingredients. The extracted active ingredients (such as charged alkaloids) migrate and accumulate in the electric field towards the electrode with the opposite charge, enhancing the subsequent low-temperature extraction. This process avoids the deactivation of heat-sensitive active substances caused by traditional heating methods. Within the specified electric field strength and treatment time, the dissolution of specific active ingredients can be achieved more effectively.

[0069] The process and mechanism of enrichment of active ingredients using electric field enrichment technology in this application include:

[0070] (1) Core characteristics of the target material

[0071] ① Differences in charge: Cationic components (such as red ginsenosides) contain amino groups (-NH3) in their molecular structure. + Positively charged components (such as Ophiopogon japonicus polysaccharide) contain carboxyl groups (-COO). - ), phenolic hydroxyl group (-O) - It carries a negative charge.

[0072] ② Water solubility compatibility: The target substance has a certain water solubility (or is soluble in the solvent system of this application (water-ethanol 3:1)), can enter the extract with the solvent flow, and is stable in the "low-pressure non-thermal environment" of electric field treatment (4kV / m-7kV / m electric field without high temperature, avoiding degradation of heat-sensitive components).

[0073] (2) Distribution of charged and neutral substances in an electric field

[0074] The migration effect of an electric field on matter depends on the "charge-electric force interaction," that is, cations are attracted to the negative electrode by the electric field and migrate to the positive electrode by the electric field. However, neutral substances have no charge and are not affected by the electric field. They are only uniformly distributed in the entire mixed slurry by the natural convection of the solvent (with slight stirring during electric field treatment), and there is no "regional enrichment" phenomenon. In this application, although neutral components (such as Ganoderma triterpenes) do not migrate with the electric field, their dissolution resistance is reduced because the electric field pretreatment can slightly damage the cell wall of the raw material (increase the pore size). They can still enter the extract synchronously with the charged target substances and are subsequently retained by the "pulse light decolorization-plasma activated water taste correction" process, without the need for additional treatment designed for their electric field distribution.

[0075] (3) Unwanted impurity characteristics

[0076] Impurities that need to be removed during the electric field treatment stage have characteristics that are clearly opposed to the target substance, forming a "charge / polarity contrast" with the target substance, which facilitates subsequent separation. These impurities are mainly divided into two categories:

[0077] ① Strongly polar inorganic impurities

[0078] For example, residual calcium ions (Ca) in raw materials (Gastrodia elata, Eucommia ulmoides) 2+ ), magnesium ions (Mg 2+ Metal cations such as ions and chloride ions (Cl) - ), sulfate (SO4 2- Inorganic anions such as Ca2+ and α2+. Although these impurities are charged, they have no effect on "relieving menopausal symptoms" and may form complexes with the target ingredient (such as Ophiopogon japonicus polysaccharide), affecting efficacy. Characteristics: High charge density (small ionic radius, such as Ca2+). 2+ Its charge density is much higher than that of red ginsenosides, and its migration rate in an electric field is fast (it tends to migrate preferentially to the electrode).

[0079] ② Hydrophobic macromolecular impurities

[0080] Examples of impurities include cellulose and lignin (neutral, uncharged) in raw materials (red ginseng, Ganoderma lucidum), and denatured proteins (which may carry a weak charge, but have a large molecular weight and poor water solubility). These impurities are inactive and can cause turbidity in the extract, affecting the color of the product. Characteristics: Neutral or weakly charged, large molecular weight, unable to pass through the cell wall pores disrupted by the electric field (the electric field only slightly disrupts the cell wall, with a pore size <100nm), making it difficult to enter the extract, and mostly remaining in the residue.

[0081] (4) Differentiated treatment of the positive, negative and intermediate regions of the electric field

[0082] ① Positive region of electric field

[0083] Because the positive electrode attracts anions, it will accumulate negatively charged inorganic impurities (such as SO4) from the raw materials. 2- Cl- And a small amount of negatively charged macromolecular impurities. Due to their large molecular weight or tight binding with the raw material matrix, these impurities mostly adhere to the surface of the raw material residue. They are removed along with the drug residue in the "200-mesh filter" step after the electric field treatment, without the need for additional special treatment for the positive electrode area.

[0084] ②Negative region of electric field

[0085] Because the negative electrode attracts cations, it accumulates metal cation impurities (such as Ca). 2+ Mg 2+ This application adjusts the pH of the extract to 6-7 (neutral range) after extraction, allowing the metal cations to react with trace amounts of carbonate (CO3) in the extract. 2- ), hydroxide ions (OH) - Insoluble precipitates (such as CaCO3 and Mg(OH)2) are formed and subsequently removed by "precision filtration before decolorization (such as 0.22μm filter membrane)" to avoid affecting the decolorization effect of pulsed light.

[0086] ③ The middle region of the electric field: retains neutral target material and charged target material that has not been completely migrated, and directly enters the subsequent process.

[0087] Although there is no obvious charge accumulation in the intermediate region, the migration of cations (such as ginsenosides) and anions (such as Ophiopogon japonicus polysaccharides) takes a certain amount of time (30-40 minutes of electric field treatment, during which they are not completely migrated to the electrode). Therefore, the extract in the intermediate region has the highest content of target substances and the fewest impurities. In this application, the mixed extract after electric field treatment (including the intermediate region and the target substances that have not been completely migrated) is directly collected, filtered, and then proceeded to the pulsed light decolorization step. No additional treatment of the intermediate region is required, thus balancing efficiency and target substance retention.

[0088] In some embodiments, the extraction temperature is 65°C-70°C, including but not limited to 65°C, 66°C, 67°C, 68°C, 69°C, 70°C or any of the foregoing ranges and values ​​within those ranges.

[0089] In some embodiments, the extraction time is 40 min to 50 min, including but not limited to 40 min, 42 min, 44 min, 46 min, 48 min, 50 min or any of the foregoing ranges and values ​​within that range.

[0090] In some embodiments, extraction is performed by adding a solvent, which includes one or more of water and ethanol. Further, this application uses water and ethanol as extraction solvents, which can better extract the active substances from the electric field-enriched feed solution and improve efficacy. As a non-limiting example, the mass ratio of water to ethanol is (2-5):1; further, the mass ratio of water to ethanol is (2.8-3.2):1.

[0091] Because food and medicine ingredients contain heat-sensitive active ingredients such as polyphenols, flavonoids, polysaccharides, and saponins, this application uses low-temperature cyclic extraction on the material after electric field treatment. Low temperature combined with low-voltage electric field pretreatment can significantly reduce the loss of active ingredients, and cyclic extraction can improve extraction efficiency and ensure yield.

[0092] In some embodiments, the extraction process further includes filtering the material to separate the residue and collecting the filtrate to form the extract. As a non-limiting example, the filtration mesh size is 100-300 mesh.

[0093] In some embodiments, the extraction step is followed by a decolorization treatment of the extract using pulsed light.

[0094] In some embodiments, the light source of the pulsed light is ultraviolet light. Further, the wavelength of the ultraviolet light is 200nm-400nm.

[0095] In some embodiments, the energy of the pulsed light is 0.1 J / cm². 2 -0.5J / cm 2 including but not limited to 0.1 J / cm 2 0.2J / cm 2 0.3J / cm 2 0.4J / cm 2 0.5J / cm 2 Or the range formed by either of the above and the values ​​within that range.

[0096] In some embodiments, the frequency of the pulsed light is 50Hz-70Hz, including but not limited to 50Hz, 55Hz, 60Hz, 65Hz, 70Hz or any combination thereof and values ​​within that range.

[0097] This application utilizes pulsed light decolorization technology to precisely degrade pigment macromolecules through physical decolorization, significantly reducing the coloring power of the product and giving it a clear and bright sensory quality.

[0098] In some embodiments, the extract flows through a thin-layer channel with a thickness of 0.5 mm to 1 mm at a flow rate of 5 mL / min to 10 mL / min. When the total color difference ΔE of the outflowing extract is ≥25 (based on the unbleached extract) as monitored by the online colorimeter, the decolorization process is stopped. At this time, the decolorized liquid has a light amber color and no turbidity.

[0099] In some embodiments, plasma-activated water is used to flavor the decolorized liquid.

[0100] In some preferred embodiments, the plasma-activated water comprises vaporized plasma-activated water.

[0101] In some preferred embodiments, the process of flavor correction using plasma-activated water is called headspace treatment. To better understand, "headspace treatment" refers to introducing vaporized plasma-activated water into the upper space of the reaction chamber, where it contacts the upper surface of the decolorizing liquid but remains insoluble in the liquid. A suitable implementation involves placing the decolorizing liquid in the sample chamber of a closed gas-phase reactor, introducing vaporized plasma-activated water to fill the "headspace" above the decolorizing liquid in the sample chamber, and allowing the plasma-activated water to contact the decolorizing liquid through gas-phase diffusion, thus correcting the odor of the decolorizing liquid. This treatment method can efficiently oxidize and degrade bitter and medicinal odor molecules in decolorizing liquids (such as bird's nest compound products), avoiding direct liquid contact that may damage the target active ingredients in the decolorizing liquid, and leaving no flavoring agent residue.

[0102] In some embodiments, the oxidation-reduction potential of the plasma-activated water is >800 mV. Further, the oxidation-reduction potential of the plasma-activated water is 800 mV-1000 mV, including but not limited to 800 mV, 850 mV, 900 mV, 950 mV, 1000 mV or any combination thereof and values ​​within that range.

[0103] In some embodiments, the temperature of the plasma-activated water is 40°C-60°C, including but not limited to 40°C, 45°C, 50°C, 55°C, 60°C, or any combination thereof and values ​​within that range. It is understood that the plasma-activated water includes vaporized plasma-activated water.

[0104] In some embodiments, the flavoring treatment time is 10 min to 30 min, including but not limited to 10 min, 15 min, 20 min, 25 min, 30 min, or any range formed by both of the foregoing and values ​​within that range. It can be understood that the flavoring treatment time can be the time for the plasma-activated water to interact with the decolorizing solution.

[0105] In a preferred embodiment, the extract obtained from extraction is first decolorized using pulsed light, and then the decolorized liquid is flavored using plasma-activated water. If flavoring is performed before decolorization, the following issues arise: the flavoring process degrades a batch of desired flavor compounds, and the subsequent decolorization process results in the loss of another batch, leading to excessive loss of flavor compounds and a ultimately bland product. Furthermore, newly generated off-flavors after decolorization (such as pigment degradation byproducts) require secondary flavoring, increasing process complexity and cost. Therefore, the embodiment of this application employs decolorization followed by flavoring, which not only preserves the desired flavor compounds but also reduces process complexity and cost, improving production quality and efficiency.

[0106] In some preferred embodiments, a triple physical processing technique combining "electric field enrichment technology - pulsed light technology - plasma technology" is employed to better preserve the heat-sensitive active ingredients in the compound product and address issues related to product color and flavor. First, electric field enrichment technology is used to pretreat the mixed slurry, achieving ion cyclic enrichment and slightly altering the permeability of the material's cell walls through the electric field, thereby reducing dissolution resistance and synergistically improving extraction efficiency. Next, pulsed light technology is used to specifically degrade pigment molecules generated by Maillard reactions, achieving precise decolorization and imparting a clear and bright sensory quality to the product. Finally, an innovative plasma-activated water technology is used to efficiently degrade and mask undesirable flavors inherent in the raw materials (such as beany or herbal tastes) as well as undesirable flavor substances generated during the decolorization process (such as pigment degradation byproducts), achieving superior flavor correction.

[0107] The preparation method of this application employs non-thermal physical processing, leaving no chemical additive residues. It also ensures high retention of core functional components (such as polysaccharides and flavonoids), and the prepared compound solution exhibits no noticeable acidity or astringency, minimizing its impact on the expansion and rehydration of bird's nest. Furthermore, the bird's nest products prepared using this process have a bright color, prominent medicinal and edible characteristics, and a smooth, full-bodied texture without bitterness.

[0108] In some embodiments, the bird's nest in step S30 includes pre-treated bird's nest. The pre-treatment step includes: soaking the bird's nest raw material in water, picking out and removing impurities such as black spots, bird feathers, and eggshells, and then washing and drying to obtain bird's nest.

[0109] In some embodiments, before mixing the compound solution with bird's nest in step S30, an excipient is added to the compound solution for dissolution. As a non-limiting example, the excipient includes food excipients or pharmaceutical excipients. As a non-limiting example, the excipient includes rock sugar. Further, the amount of rock sugar added in the compound product is 5-15 parts by weight. As a non-limiting example, the dissolution temperature is 85℃-90℃.

[0110] In some embodiments, the mixture of the compound solution and bird's nest is further subjected to sterilization. As a non-limiting example, the sterilization temperature is 115°C-125°C. As a non-limiting example, the sterilization time is 15-20 minutes.

[0111] In some preferred embodiments, the mixture is further subjected to a weighing process before sterilization. It is understood that the weighing process is a process to standardize the concentration of the mixture. As a non-limiting example, the weighing process includes a concentration process. Optionally, the concentration temperature is 50°C-60°C, and the concentration vacuum degree is -0.08 MPa to -0.09 MPa.

[0112] Thirdly, this application also provides the application of a bird's nest compound product in the preparation of a formulation to relieve menopausal symptoms in women. The bird's nest compound product includes the bird's nest compound product with the effect of improving women's health provided in the first aspect or the bird's nest compound product with the effect of improving women's health prepared by the preparation method provided in the second aspect.

[0113] In some embodiments, the symptoms of female menopause include one or more of the following: uterine and ovarian insufficiency, hot flashes and night sweats, and decreased sleep quality.

[0114] The bird's nest compound product provided in this application can significantly improve menopausal symptoms in women, including but not limited to relieving hot flashes and night sweats, regulating hormone levels, improving sleep quality, enhancing ovarian function, and improving uterine function, among other symptoms, with a mild effect and high safety. The bird's nest compound product has excellent flavor, good color, and high activity, meeting the consumer demand for efficient, convenient, and natural health products.

[0115] It should be noted that the test methods in the following embodiments of this application, unless otherwise specified, are generally performed under conventional conditions or as recommended by the manufacturer. All commonly used chemical reagents used in the embodiments are commercially available products, or can be prepared by those skilled in the art using known methods.

[0116] Example 1

[0117] The ingredients for the compound bird's nest product are: 3 grams of bird's nest, 6 grams of red ginseng, 6 grams of gastrodia elata, 9 grams of ophiopogon japonicus, 6 grams of donkey-hide gelatin, 12 grams of eucommia ulmoides leaves, 1 gram of ganoderma lucidum, and 5 grams of rock sugar.

[0118] The bird's nest compound product is prepared according to the above raw material formula using the following preparation method, with the specific steps as follows:

[0119] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0120] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0121] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 7.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1) as the extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0122] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5mm-1mm, peristaltic pump driven flow rate 5mL / min, channel length 30cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0123] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0124] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0125] Example 2

[0126] The ingredients for the compound bird's nest product are: 10g bird's nest, 12g red ginseng, 12g gastrodia elata, 15g ophiopogon japonicus, 12g donkey-hide gelatin, 20g eucommia ulmoides leaves, 10g ganoderma lucidum, and 15g rock sugar.

[0127] The bird's nest compound product is prepared according to the above raw material formula using the following preparation method, with the specific steps as follows:

[0128] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0129] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0130] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 7.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0131] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5mm-1mm, peristaltic pump driven flow rate 5mL / min, channel length 30cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0132] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0133] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0134] Example 3

[0135] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0136] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0137] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0138] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 4.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0139] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5mm-1mm, peristaltic pump driven flow rate 5mL / min, channel length 30cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0140] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0141] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0142] Example 4

[0143] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0144] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0145] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0146] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 7.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0147] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 200 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0148] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0149] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0150] Example 5

[0151] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0152] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0153] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0154] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 7.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0155] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0156] Plasma-activated water vapor phase flavoring: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 40°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0157] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0158] Example 6

[0159] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0160] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0161] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0162] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber (channel thickness 0.5-1mm, peristaltic pump drive flow rate 5mL / min, channel length 30cm), and an electric field strength of 7.0 kV / m is applied for treatment for 30min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank, and water and ethanol (3:1, w / w) are used as extraction solvents for extraction. The temperature is raised to 70℃, and the extraction is carried out for 40min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0163] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 400 nm and an energy of 0.5 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0164] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0165] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0166] Example 7

[0167] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0168] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0169] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0170] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 10.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0171] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0172] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0173] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0174] Example 8

[0175] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0176] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0177] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0178] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 7.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0179] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 400 nm and an energy of 0.8 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0180] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0181] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0182] Example 9

[0183] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0184] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0185] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0186] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 7.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0187] Decolorization treatment: The above extract was passed through a pulsed light treatment device in thin-layer form, using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0188] Plasma-activated water vapor phase flavoring: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. Plasma-activated water with a pre-prepared oxidation-reduction potential (ORP) of 600 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0189] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0190] Comparative Example 1

[0191] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0192] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0193] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0194] Circulating extraction process: The mixed slurry is transferred to a circulating extraction tank, and water and ethanol (3:1, w / w) are used as extraction solvents for extraction. The temperature is raised to 70℃, and the circulating extraction is carried out for 40 minutes. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0195] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0196] Plasma-activated water vapor phase deodorization: The above decolorizing liquid is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0197] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain the compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal it, and sterilize it at 115°C for 20 minutes to obtain the bird's nest compound product.

[0198] Comparative Example 2

[0199] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0200] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0201] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0202] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 10.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0203] Plasma-activated water vapor phase flavoring: The above extract is placed in the sample chamber of a closed vapor phase reactor. The pre-prepared plasma-activated water with an oxidation-reduction potential (ORP) > 800 mV is vaporized at 60°C. The gaseous plasma-activated water is then introduced into the sample chamber of the vapor phase reactor to perform headspace treatment on the decolorizing liquid for 20 min to obtain the final extract.

[0204] Mixing, filling, and sterilization: Add rock sugar to the final extract and heat together to 90°C until completely dissolved. Determine the weight to obtain a compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to certain specifications, seal the container, and sterilize at 115°C for 20 minutes to obtain the bird's nest compound product.

[0205] Comparative Example 3

[0206] The bird's nest compound product was prepared using the raw material formula of the bird's nest compound product in Example 1, and the specific steps are as follows:

[0207] Bird's nest raw material processing: Soak the pure bird's nest raw material in pure water for 2 hours, pick out and remove impurities such as black spots, bird feathers, and eggshells, then wash, spin dry, and dry to obtain bird's nest.

[0208] After mixing the red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves and ganoderma lucidum according to the formula, add 50 times the weight of room temperature purified water and stir for 10 minutes. Adjust the pH to 6.5 to form a mixed slurry.

[0209] Low-voltage electric field enrichment technology treatment: The mixed slurry is pumped into the pulse electric field treatment chamber and treated with an electric field strength of 10.0 kV / m for 30 min. Then, the mixed slurry after electric field treatment is transferred to the circulating extraction tank and extracted with water and ethanol (3:1, w / w) as extraction solvent. The temperature is raised to 70℃ and the extraction is carried out for 40 min. After the extraction is completed, the residue is separated by filtering through a 200-mesh filter to obtain the extract.

[0210] Decolorization treatment: The above extract was flowed in thin-layer form through a pulsed light treatment device (channel thickness 0.5-1 mm, peristaltic pump driven flow rate 5 mL / min, channel length 30 cm), using a wavelength of 400 nm and an energy of 0.2 J / cm². 2 The decolorizing solution is obtained by 10 cycles of pulsed light with a pulsed light frequency of 60Hz and a single irradiation time of 7.2s. Under normal circumstances, the total color difference of the decolorizing solution obtained at this time can meet the standard for the end of the decolorization process, with a total color difference ΔE≥25.

[0211] Mixing, filling, and sterilization: Add the prescribed amount of rock sugar to the above decolorizing solution and heat together to 90°C until completely dissolved. Determine the weight to obtain a compound solution. Then, place the prepared bird's nest and the compound solution into a packaging container according to a certain specification, seal the container, and sterilize at 115°C for 20 minutes to obtain the bird's nest compound product.

[0212] Experimental Example 1: Sensory Evaluation of Compound Bird's Nest Products

[0213] Sensory evaluations were conducted on the bird's nest compound products prepared in Examples 1-9 and Comparative Examples 1-3, and taste evaluations were performed using an electronic tongue. The relative bitterness of the products was presented in data form, with higher bitterness scores indicating more pronounced bitterness. The sensory evaluation scores and results are shown in Table 1.

[0214] Table 1: Sensory evaluation scores and results of bird's nest compound products

[0215]

[0216] As shown in Table 1, within the preferred process parameters (such as Examples 1-6 and some examples), the bird's nest compound product prepared in this application has a good appearance and color, possesses the comprehensive flavor characteristics of food and medicine homology ingredients, has a mellow and smooth taste, no obvious bitterness, and a smooth and full-bodied bird's nest texture. However, when the process parameters exceed the preferred range (such as the excessively high electric field strength in Example 7, the excessive pulse light energy in Example 8, and the insufficient activation water potential in Example 9), the overall quality of the product will decline to varying degrees. The bird's nest compound products prepared in Comparative Examples 1-3 exhibited significant defects in overall quality: Comparative Example 1, lacking low-voltage electric field pretreatment, had a low cell wall breakage rate, insufficient extraction of active ingredients, resulting in a dark brown and slightly cloudy finished product with a bitterness value as high as 9.1±0.5 and a rough flavor; Comparative Example 2, lacking pulsed light decolorization treatment, had a large amount of residual pigment from the Maillard reaction, resulting in a dark brown and noticeably cloudy appearance. Although flavor correction was performed, the dull sensory effect caused by the pigment could not be improved, leading to a poor overall color; Comparative Example 3, without plasma flavor correction treatment, failed to degrade the inherent bitterness of the raw materials, causing the bitterness value to soar to 9.5±0.6, and a significant decrease in umami / richness, resulting in a dry mouthfeel. Even with a clear color, the flavor defects could not be concealed. None of the three met the basic quality requirements of "light amber color and mellow flavor." The sensory characteristics of the bird's nest compound products prepared in Examples 7-9 were slightly lower than those in Examples 1-6, but were still significantly better than those prepared in Comparative Examples 1-3.

[0217] Experimental Example 2: Detection of Active Ingredient Content in Compound Bird's Nest Products

[0218] The total polysaccharides, total flavonoids, and saponins in the bird's nest compound products prepared in Examples 1, 3, 7, and Comparative Example 1 were detected, and the percentage increase in the retention rate of total polysaccharides, total flavonoids, and saponins was calculated as follows:

[0219]

[0220] Where: m1 - the measured content of active ingredient in a certain sample, mg / g; m0 - the measured content of active ingredient in Comparative Example 1, mg / g.

[0221] Five parallel tests were conducted, and the average value was taken. The results are shown in Table 2.

[0222] Table 2: Effects of Low-Voltage Electric Field Enrichment Technology on the Content of Effective Components in Compound Bird's Nest Products

[0223]

[0224] The data in Table 2 show that the content of active ingredients in the bird's nest compound products prepared using low-voltage electric field enrichment technology in Examples 1, 3, and 7 is higher than that in Comparative Example 1, which did not use this technology. The main reason for this is that the electric field enhances the dissolution of target ingredients by inducing ion enrichment cycles and increasing cell permeability, thereby significantly improving the extraction efficiency of subsequent low-temperature extraction processes.

[0225] Example 3: Appearance evaluation of bird's nest compound product

[0226] Sensory preferences and color differences of the bird's nest compound products prepared in Examples 1, 4, 6, 8, and Comparative Example 2 were evaluated. A professional evaluation team of 10 people was formed to evaluate color preferences. The evaluation subjects were presented in the form of "random codes". The evaluation team had to independently observe the color of the samples and score them on a scale of 1-10 based on three core indicators: "clarity, color uniformity, and light amber color fit" (1 point - dark and turbid color, completely deviating from light amber color; 10 points - clear and bright color, perfectly matching the optimal light amber color). The higher the color preference score, the better the color. The total color difference ΔE was calculated with Comparative Example 2 as the baseline (0). The calculation formula is as follows:

[0227]

[0228] In the formula: - Difference in brightness; -Red-green hue; -Yellow-blue color.

[0229] The larger the total color difference value from the reference, the better the decolorization effect. Brightness was tested using a colorimeter; a higher value indicates a brighter and more transparent sample, while a lower value indicates a darker sample. Five parallel tests were performed, and the average value was taken. The results are shown in Table 3. See the product image below. Figure 1 .

[0230] Table 3: Evaluation results of the appearance of bird's nest compound products after pulsed light decolorization

[0231]

[0232] The data in Table 3 show that the pulsed light decolorization technology used in this application can effectively improve the appearance of bird's nest compound products, enhance product brightness and color appeal, and reduce brownness.

[0233] Example 4: Odor Evaluation of Bird's Nest Compound Products

[0234] The overall preference, flavor, and sensory characteristics of the bird's nest compound products prepared in Examples 1, 5, 9, and Comparative Example 3 were evaluated. The overall preference score was out of 10, with higher scores indicating better aroma. Bitterness, astringency, and umami / richness were tested using an electronic tongue. Five parallel tests were conducted, and the average value was taken. The results are shown in Table 4.

[0235] Table 4: Evaluation Results of Odor of Bird's Nest Compound Products by Plasma-Activated Water Flavoring Technology

[0236]

[0237] The data in Table 4 show that plasma-activated water vapor phase flavoring technology can effectively reduce bitterness, thereby increasing the smoothness and richness of the product's taste and achieving a higher overall preference score.

[0238] In summary, the evaluation and testing results of the comprehensive sensory properties and active ingredient content of the bird's nest compound products in Examples 1-4 show that the preparation method of this application can effectively improve the activity, appearance and sensory effects of the bird's nest compound products, and the preparation method has obvious advantages.

[0239] Experimental Example 5: Efficacy Evaluation of Compound Bird's Nest Products

[0240] This experiment evaluated the efficacy of bird's nest compound products with different ingredient formulations. Specific samples are as follows:

[0241] Formula 1: 3g bird's nest, 6g red ginseng, 6g gastrodia elata, 9g ophiopogon japonicus, 6g donkey-hide gelatin, 12g eucommia ulmoides leaves, 1g ganoderma lucidum, and 5g rock sugar.

[0242] Formula 2: 3g bird's nest, 15g prepared rehmannia root, 12g privet fruit, 12g eclipta prostrata, 9g angelica sinensis, 12g white peony root, 6g bupleurum root, 15g poria cocos, 9g salvia miltiorrhiza, 30g wheat bran, 6g prepared licorice root, and 5g rock sugar.

[0243] The preparation methods for the two groups of bird's nest compound products are the same as in Example 1.

[0244] ① Grouping of experimental animals and establishment of models:

[0245] Eighty female SPF-grade ICR mice, five per cage, were housed at a temperature of 25°C and a humidity of 50%, with a 12-hour day-night cycle. The animals had free access to food and water. In the naturally aging group, vaginal smears were examined, and the estrous cycle was determined to be 7-8 days or even more than 10 days. Vaginal exfoliative cytology was used to observe the estrous cycle and frequency to assess whether the mice had entered perimenopause.

[0246] ② General observation:

[0247] Observe behavioral changes in the model group mice, including their mental state, activity frequency, and fur color. Collect vaginal exfoliated cell smears to observe changes in the animals' estrous cycle, using this as a basis for determining the success of the model establishment.

[0248] In mice, cell types vary at different stages of the estrous cycle: prophase is dominated by nucleated cells, phase II by anucleated keratinocytes, and late II by a coexistence of all three (nucleated epithelial cells, keratinocytes, and leukocytes), while interphase is dominated by leukocytes. The control group maintained normal estrous cycle changes; however, observation of the model group (naturally aging) for 5 consecutive days showed that the mice lost their original cycle regularity and remained in the interestrous phase, marking the successful establishment of the model (see Appendix). Figure 2 ).

[0249] (1) Effects of different raw material formulations of bird's nest compound products on serum E2, LH and FSH levels

[0250] Efficacy tests were conducted on two groups of bird's nest compound products. This study measured serum estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels in mice to objectively evaluate the key endocrine indicators of the intervention's regulatory effect on the hypothalamic-pituitary-ovarian axis (HPO axis) function. The specific experimental procedures are as follows:

[0251] Mice that successfully entered the perimenopausal model were selected for the experiment. Mice in treatment group 1 were administered a self-prepared bird's nest formula (Formula 1) by gavage, while mice in treatment group 2 were administered Formula 2 by gavage. This gavage was repeated for 4 weeks. The control group and the model group were administered an equal volume of water by gavage. Blood was collected, and plasma was centrifuged at 3500 r / min for 10 min. The supernatant was collected and stored at -80 ℃ for later use. Ovarian and uterine tissues were collected, weighed, and stored at -80 ℃.

[0252] Efficacy testing indicators: Serum E2, LH, and FSH levels were measured by ELISA, strictly following the instructions for use of the kit (Jiangsu Enzyme Immunization Industry Co., Ltd.).

[0253] Estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) are key hormones for evaluating ovarian function and the regulatory status of the hypothalamus-pituitary-ovarian axis. Studies have shown that serum E2 levels decrease during menopause or in a state of ovarian dysfunction, while FSH and LH levels significantly increase.

[0254] See appendix Figures 3-5 The results showed that compared with the control group, the serum E2 level in the model group mice was significantly reduced, while the FSH and LH levels were significantly increased, indicating impaired ovarian function and successful establishment of the menopausal model. Compared with the model group, treatment groups 1 and 2 all showed varying degrees of increase in E2 and decrease in LH levels. Among them, treatment group 1 showed a greater recovery in all indicators, closer to the levels of the control group, while the FSH value in treatment group 2 was even higher than that in the model group. In conclusion, under the same process conditions, formula 1 is more effective than formula 2 in improving ovarian endocrine function and alleviating menopausal-related hormonal imbalances.

[0255] (2) Effects of different dosage groups of bird's nest compound products on serum E2, LH and FSH levels

[0256] Mice that successfully entered the perimenopausal model were randomly divided into 5 groups, with 10 mice in each group. They were administered the compound bird's nest product of Formula 1 at doses of 14.01 g / kg (low-dose group), 28.02 g / kg (medium-dose group), and 42.03 g / kg (high-dose group) via gavage for 4 consecutive weeks. The control group and the model group were administered the same amount of water via gavage. Blood was collected, and plasma was centrifuged at 3500 r / min for 10 min. The supernatant was collected and stored at -80℃ for later use. Ovarian and uterine tissues were collected, weighed, and stored at -80℃.

[0257] Serum E2, LH, and FSH levels were measured using ELISA. The serum E2, LH, and FSH levels were measured strictly according to the instructions of the kit (Jiangsu Enzyme Immunoassay Co., Ltd.). See the appendix for the results of serum E2, LH, and FSH hormone levels. Figure 6 To be continued Figure 8The results showed that medium-dose bird's nest compound products effectively reduced luteinizing hormone (LH) levels in mice, while high-dose products increased estradiol (E2) levels, and low-dose products reduced follicle-stimulating hormone (FSH) levels. In summary, compared with the model group, different doses of bird's nest compound products could regulate hormone levels to varying degrees and alleviate menopausal-related hormonal imbalances.

[0258] (3) Effects of different dosage groups of bird's nest compound products on sleep latency

[0259] A sodium pentobarbital sleep latency experiment was conducted using bird's nest compound products at different dosage groups. The bird's nest compound products were administered at doses of 14.01 g / kg (low dose), 28.02 g / kg (medium dose), and 42.03 g / kg (high dose). The control group and model group were administered the same amount of water by gavage. This gavage intervention lasted for 4 weeks. Twenty minutes after the last administration, sodium pentobarbital was injected intraperitoneally at a dose of 280 mg / kg (ensuring 100% sleep in mice). The sleep latency was defined as the time from the intraperitoneal injection to the disappearance of the righting reflex, measured by 60 seconds. The differences in sleep latency among the control group, model group, and bird's nest group were compared.

[0260] See appendix for results. Figure 9 The results showed that, compared with the model group, the bird's nest compound products of different dosage groups could shorten the sleep latency of mice. Among them, compared with the high dosage group, the low and medium dosage groups could further shorten the sleep latency of mice and effectively improve sleep quality.

[0261] (4) Effects of different dosage groups of bird's nest compound products on uterine morphology

[0262] Mouse uteri were harvested, rinsed with physiological saline, and fixed in 10% neutral formaldehyde. The morphology of the endometrium was observed using hematoxylin and eosin (HE) staining. Results are shown in the appendix. Figure 10 As shown, the results indicated that the bird's nest compound products in different dosage groups (low dose group 14.01g / kg, medium dose group 28.02g / kg, and high dose group 42.03g / kg) could thicken the endometrium, repair the endometrial and stromal layers, and increase the number of glands, thus having a repairing effect on uterine damage; among them, the medium and high doses had more obvious repair effects on uterine damage.

[0263] (5) Effects of different dosage groups of bird's nest compound products on ovarian morphology

[0264] Mouse ovaries were harvested, rinsed with physiological saline, and fixed in 10% neutral formaldehyde. The number of follicles and the morphology of the ovaries were observed using hematoxylin and eosin (HE) staining. See the appendix for the H&E staining results of ovarian sections. Figure 11(As shown in the figure, 1 represents primordial follicles; 2 represents primary follicles; 3 represents secondary follicles; and 4 represents mature follicles.) The results showed that different dose groups (low dose group 14.01g / kg, medium dose group 28.02g / kg, and high dose group 42.03g / kg) of the bird's nest compound product could increase the number of primordial, primary, and secondary follicles in the ovary and reduce the number of cystic follicles, thus improving follicular damage. Among them, the high dose could further increase the number of secondary follicles and had a certain effect on ovarian repair.

[0265] (6) Effects of different dosage groups of bird's nest compound products on sweat secretion

[0266] Sweat staining was used to determine sweat secretion in the plantar lobes of mice. Wada-Takagaki reagent A (2g iodine dissolved in 100mL anhydrous ethanol) and reagent B (50g soluble starch mixed with 100mL castor oil) were prepared. Thirty minutes after gavage administration of different dose groups (low dose 14.01g / kg, medium dose 28.02g / kg, high dose 42.03g / kg) of the bird's nest compound product, the mice were fixed and their plantar lobes were dried. Solution A was applied sequentially, allowed to dry, and then solution B was applied. The dark purple sweat spots were observed and recorded. The sweating status of the mouse plantar lobes was observed. (See Appendix) Figure 12 The results showed that the observation and analysis indicated that all dosage groups of the bird's nest formula had an inhibitory effect on sweat secretion, which alleviated the symptoms of hot flashes and night sweats to a certain extent.

[0267] In summary, the bird's nest compound product provided in this application achieves a favorable "adjuvant and principal" combination of medicinal and edible materials. By combining bird's nest with medicinal and edible materials, a bird's nest compound product is obtained that can significantly improve menopausal syndrome, enhance uterine and ovarian function, effectively regulate hormone levels, improve sleep quality, promote ovarian and uterine repair, and relieve symptoms such as hot flashes and night sweats. Moreover, it has a mild effect, high safety, good flavor, and good color.

[0268] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0269] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A method for preparing a compound bird's nest product with effects on improving women's health, characterized in that, Includes the following steps: Red ginseng, gastrodia elata, ophiopogon japonicus, donkey-hide gelatin, eucommia ulmoides leaves, and ganoderma lucidum are mixed with a solvent to form a mixed slurry; The mixed slurry is treated and extracted by applying an electric field with an intensity of 4kV / m-7kV / m. The extracted liquid is decolorized by pulsed light, and the decolorized liquid is flavored by plasma-activated water to prepare a compound solution. The compound solution is mixed with bird's nest to prepare a bird's nest compound product that improves women's health. By weight, the raw materials of the bird's nest compound product include 3-10 parts bird's nest, 6-12 parts red ginseng, 6-12 parts gastrodia elata, 9-15 parts ophiopogon japonicus, 6-12 parts donkey-hide gelatin, 12-20 parts eucommia ulmoides leaves and 1-10 parts lingzhi mushroom. The source of the pulsed light is ultraviolet light, and the energy of the pulsed light is 0.1 J / cm². 2 -0.5J / cm 2 The frequency of the pulsed light is 50Hz-70Hz.

2. The preparation method of the bird's nest compound product with the effect of improving women's health according to claim 1, characterized in that, The solvent includes water.

3. The preparation method of the bird's nest compound product with the effect of improving women's health according to claim 1, characterized in that, The total mass ratio of the red ginseng, the gastrodia elata, the ophiopogon japonicus, the donkey-hide gelatin, the eucommia ulmoides leaf, and the ganoderma lucidum to the solvent is 1:(30-50).

4. The method for preparing the bird's nest compound product with the effect of improving women's health according to any one of claims 1 to 3, characterized in that, The processing time for the electric field is 30-40 minutes.

5. The method for preparing a bird's nest compound product with effects on improving women's health according to any one of claims 1 to 3, characterized in that, The extraction solvent includes one or more of ethanol and water; and / or, the extraction temperature is 65℃-75℃; and / or, the extraction time is 40min-50min.

6. The method for preparing the bird's nest compound product with the effect of improving women's health according to any one of claims 1 to 3, characterized in that, The plasma-activated water has an oxidation-reduction potential >800mV; and / or the temperature of the plasma-activated water is 40℃-60℃; and / or the flavoring treatment time is 10min-30min.

7. A compound bird's nest product with effects on improving women's health, characterized in that, It is prepared by the preparation method of the bird's nest compound product with the effect of improving women's health as described in any one of claims 1 to 6.

8. The application of a compound bird's nest product in the preparation of formulations for preventing or improving menopausal symptoms in women, characterized in that, The bird's nest compound product includes the bird's nest compound product with the effect of improving women's health as described in claim 7, or the bird's nest compound product with the effect of improving women's health prepared by the preparation method described in any one of claims 1 to 6.

9. The application according to claim 8, characterized in that, The symptoms of menopause in women include one or more of the following: uterine and ovarian insufficiency, hot flashes and night sweats, and decreased sleep quality.

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