Accurate grafting method based on vascular bundle configuration

Abstract

The invention discloses an accurate grafting method based on vascular bundle configuration, and the method comprises the steps: firstly obtaining a microscopic image of a rootstock section, and recognizing the vascular bundle configuration features of the rootstock section; acquiring a scion section microscopic image, and identifying vascular bundle configuration characteristics of the scion section; overlapping the microscopic image of the section of the rootstock and the microscopic image of the section of the scion, and pre-matching vascular bundle configurations of the scion and the rootstock by adopting an image analysis technology by taking maximization of the overlapping area of vascular bundle configuration characteristics of the rootstock and the scion as a target; after the pre-matching is completed, marking pose characterization data in the microscopic images of the sections of the scion and the rootstock at the moment; the marked pose characterization data is subjected to coordinate change and is converted into a coordinate system of a grafting device, so that the grafting device is guided to adjust the pose of the scion and / or the stock and complete the butt joint of the scion and the stock, the vascular bundle overlapping area of the scion and the stock is maximum, the vascular bundles are accurately aligned, the contact area is maximized, and the grafting quality is improved. The grafting effect is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of grafting, and particularly relates to a precise grafting method based on vascular bundle configuration. BACKGROUND

[0002] Grafting is a key technology for improving the stress resistance, yield and quality of crops. Traditional grafting methods mainly rely on the experience of operators, and the cut surfaces of the stock and the scion are aligned by visual observation, without considering the essential difference in the vascular bundle configuration of the two, which leads to a high rate of misalignment of the vascular bundles and directly affects the transport efficiency of water and nutrients, and the contact area of the grafting layer is insufficient, which easily causes problems such as low survival rate of grafted seedlings and long hardening period. In the prior art, although there are improvements in grafting tools, the core demand of matching the cut surface vascular bundle configuration has not been met. Some precise grafting researches focus on the flatness of the cut surface, but the problem of the survival rate of the interface caused by the difference in the vascular bundle structure has not been solved. Therefore, there is an urgent need for a grafting method based on the analysis and matching of the vascular bundle configuration to realize the precise alignment of the vascular bundles and maximize the contact area, thereby improving the grafting effect. SUMMARY

[0003] The purpose of the present application is to overcome the shortcomings of the prior art and provide a precise grafting method based on the vascular bundle configuration.

[0004] The present application is realized by the following technical solutions:

[0005] A precise grafting method based on the vascular bundle configuration comprises the following steps:

[0006] Step 1: Obtain a micrograph of the cut surface of the stock, and identify the vascular bundle configuration features of the cut surface of the stock;

[0007] Step 2: Obtain a micrograph of the cut surface of the scion, and identify the vascular bundle configuration features of the cut surface of the scion;

[0008] Step 3: Superimpose the micrograph of the cut surface of the stock and the micrograph of the cut surface of the scion, and use image analysis technology to pre-match the vascular bundle configurations of the scion and the stock, with the maximum overlap area of the vascular bundle configuration features of the stock and the scion as the target;

[0009] Step 4: After pre-matching, mark the pose representation data in the micrographs of the cut surfaces of the scion and the stock at this time under the pre-matching state, which is used to provide guidance for the subsequent actual docking of the scion and the stock;

[0010] Step 5: Change the pose representation data marked in step 4 to the coordinate system of the grafting device, and then guide the grafting device to adjust the pose of the scion and / or the stock, complete the docking of the scion and the stock, and maximize the overlap area of the vascular bundles of the scion and the stock;

[0011] Step 6: After docking, the docking part is fixed.

[0012] In the above technical solution, the vascular bundle configuration characteristics of the cut surface of the stock include characteristic data of vascular bundle profile and distribution.

[0013] In the above technical solution, the vascular bundle configuration characteristics of the cut surface of the scion include characteristic data of vascular bundle profile and distribution.

[0014] In the above technical solution, during the pre-matching, the area of the overlapping region of the vascular bundle configuration characteristics between the stock and the scion accounts for more than 70% of the total area of the vascular bundle of the cut surface of the stock. If this requirement cannot be met, the subsequent grafting work is not performed, and the scion or the stock is replaced, and the pre-matching is performed again.

[0015] In the above technical solution, the pose representation data in the marked micrograph of the cut surface of the scion and the stock include the center of the elliptical cut surface of the scion and the stock, the direction of the major axis, and the position of the intersection of the two major axes of the scion and the stock in the micrograph of the cut surface of the stock.

[0016] In the above technical solution, the elastic grafting clamp or the sprayed quick-curing glue is used to fix the docking part.

[0017] The advantages and beneficial effects of the present application are as follows:

[0018] The present application realizes the maximum alignment of the vascular bundles of the stock and the scion by pre-analyzing the vascular bundle configuration characteristics of the cut surface of the stock and the scion, and then adopting the operation process of "vascular bundle configuration pre-matching-positioning marking-maximizing docking", thereby increasing the contact area of the cambium, solving the problem of low nutrient transport efficiency and unstable grafting survival rate caused by the misalignment of sieve tubes and vessels in traditional grafting, and significantly improving the survival rate of grafted seedlings and the growth performance in the later period. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is the implementation flowchart of the precise grafting method based on vascular bundle configuration.

[0020] Figure 2 It is a structural schematic diagram of a grafting device.

[0021] Figure 3 It is a structural schematic diagram of a scion clamping and docking pose adjusting unit of the grafting device.

[0022] Figure 4 It is a structural schematic diagram of a stock clamping unit of the grafting device.

[0023] For ordinary skilled persons in the art, other related drawings can be obtained according to the above drawings without creative labor. DETAILED DESCRIPTION

[0024] In order for those skilled in the art to better understand the technical scheme of the present application, the technical scheme of the present application will be further described below in combination with specific embodiments.

[0025] Embodiment one

[0026] This embodiment designs a precise grafting method based on vascular bundle configuration, see the attached Figure 1 , including the following steps:

[0027] Step 1: Selecting 3-4 leaf stage of rootstock seedlings, cutting at 45 degrees angle at 1-1.5 cm below the cotyledon node, obtaining the micrograph of the rootstock section by micro-imaging technology, and identifying the vascular bundle configuration characteristics of the rootstock section. Preferably, the vascular bundle configuration characteristics include: characteristic data of vascular bundle profile and distribution.

[0028] Step 2: Selecting the same leaf stage of scion seedlings, cutting at 45 degrees angle at 0.8-1.2 cm above the cotyledon node, obtaining the micrograph of the scion section by micro-imaging technology, and identifying the vascular bundle configuration characteristics of the scion section. Preferably, the vascular bundle configuration characteristics include: characteristic data of vascular bundle profile and distribution.

[0029] Step 3: Superimposing the micrograph of the rootstock section and the micrograph of the scion section, maximizing the overlapping area of the vascular bundle configuration characteristics of the rootstock and the scion as the target, and using image analysis technology to pre-match the vascular bundle configuration of the scion and the rootstock.

[0030] Further, during pre-matching, it is necessary to ensure that the overlapping area of the vascular bundle configuration characteristics between the rootstock and the scion accounts for more than 70% of the total area of the vascular bundle of the rootstock section. If this requirement cannot be met, the subsequent grafting work will not be carried out, and the scion seedling or the rootstock seedling will be replaced, and the pre-matching will be carried out again.

[0031] Step 4: After pre-matching is completed, in this pre-matching state, the pose representation data in the micrograph of the scion and the rootstock section at this time is marked, which is used to provide guidance for the subsequent actual butt joint of the scion and the rootstock, so that the actual butt joint of the scion and the rootstock reaches this pre-matching state.

[0032] Preferably, the marked pose representation data in the micrograph of the scion and the rootstock section includes: the center of the elliptical section of the scion and the rootstock, the direction of the major axis, and the position of the intersection of the two major axes of the scion and the rootstock in the micrograph of the rootstock section.

[0033] Step 5: The pose representation data marked in step 4 is changed in coordinates and converted to the coordinate system of the grafting device, and then the grafting device is guided to adjust the pose of the scion and / or the rootstock, and the butt joint of the scion and the rootstock is completed, so that the overlapping area of the vascular bundle of the scion and the rootstock is maximized.

[0034] Step 6: After the butt joint, use elastic grafting clamp or spray quick curing glue to fix the butt joint, ensure that the butt joint surface has no displacement, and then place it in a suitable healing environment for healing period management of the grafted seedling.

[0035] Example Two

[0036] This embodiment provides a grafting device for implementing the precise grafting method described in Example One, which is described below with reference to the accompanying Figure 2 -Appendix Figure 4 , which specifically introduces the grafting device.

[0037] The grafting device comprises a mounting frame 1, a scion clamping and butt joint position adjusting unit 2 and a stock clamping unit 3 are arranged on the mounting frame 1, and the scion clamping and butt joint position adjusting unit 2 and the stock clamping unit 3 are arranged in an up-down manner.

[0038] The scion clamping and butt joint position adjusting unit 2 comprises a scion clamp 21, a transverse adjusting mechanism 22, a longitudinal adjusting mechanism 23 and a vertical movement sliding table 24, wherein the vertical movement sliding table 24 serves as a mounting base, the scion clamp 21, the transverse adjusting mechanism 22 and the longitudinal adjusting mechanism 23 are all arranged on the vertical movement sliding table 24, the vertical movement sliding table 24 can be vertically moved up and down in a pneumatic or electric manner (for example, in a pneumatic sliding table manner or an electric screw sliding table manner), thereby realizing the vertical butt joint movement of the scion and the stock; the scion clamp 21 is used for clamping the scion clamp 100, the scion clamp 21 is mounted on the vertical movement sliding table 24 through the transverse adjusting mechanism 22 and the longitudinal adjusting mechanism 23, the transverse position of the scion clamp 21 is fine-tuned through the transverse adjusting mechanism 22, and the longitudinal position of the scion clamp 21 is fine-tuned through the longitudinal adjusting mechanism 23, so as to maximize the vascular bundle overlapping area when the scion 100 and the stock 200 are butt jointed, and improve the grafting quality.

[0039] Further, as a preferred embodiment, the transverse adjusting mechanism 22 comprises a first motor 221, a first gear 222, a transverse rack 223 and a transverse sliding plate 224, the first motor 221 is fixedly installed on the vertical movement sliding table 24, the transverse sliding plate 224 is slidably installed on the vertical movement sliding table 24 through a transverse rail 225, the transverse rack 223 is fixedly installed on the side of the transverse sliding plate 224, the first gear 222 is engaged with the transverse rack 223, and the first gear 222 is installed on the output shaft of the first motor 221; when working, the first motor 221 drives the transverse sliding plate 224 to move transversely through the first gear 222 and the transverse rack 223.

[0040] Further, as a preferred embodiment, the longitudinal adjusting mechanism 23 comprises a second motor 231, a second gear 232, a longitudinal rack 233, a longitudinal sliding plate 234 and a longitudinal fixed mounting plate 235, the longitudinal fixed mounting plate 235 is mounted on the transverse sliding plate 224 of the transverse adjusting mechanism 22 and moves synchronously therewith, the second motor 231 is fixedly mounted on the longitudinal fixed mounting plate 235, the longitudinal sliding plate 234 is longitudinally slidingly mounted on the longitudinal fixed mounting plate 235, the longitudinal rack 233 is fixedly mounted on the longitudinal sliding plate 234, the second gear 232 is engaged with the longitudinal rack 233, and the second gear 232 is mounted on the output shaft of the second motor 231, in operation, the second motor 231 drives the longitudinal sliding plate 234 to move longitudinally through the second gear 232 and the longitudinal rack 233.

[0041] The stock clamping unit 3 comprises a stock clamp 31 for clamping the stock.

[0042] The grafting device further comprises a stock rotating seat 6, the bottom of the stock is placed on the stock rotating seat 6, and the circumferential angle of the stock 200 is adjusted through the stock rotating seat 6. It should be noted that when the circumferential angle of the stock 200 is adjusted, the stock clamping unit 3 is in a relaxed state, and the stock is clamped after the adjustment angle is completed, so that the stock clamping unit 3 and the stock rotating seat 6 cooperate with each other to realize the adjustment of the stock 200 to the required angle and clamping and fixing.

[0043] The grafting device further comprises a first microscopic imaging unit 4 for microscopic imaging of the cut surface of the stock 200.

[0044] The grafting device further comprises a second microscopic imaging unit 5 for microscopic imaging of the cut surface of the scion 100.

[0045] For ease of explanation, spatially relative terms such as "upper", "lower", "left", "right", and the like, are used in the embodiments to describe one element's or feature's relationship to another element or feature as illustrated in the figures. It will be understood that the spatial terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device is inverted, elements described as being on the "lower" side of other elements or features would then be oriented on "upper" sides of the other elements or features. Accordingly, the exemplary terms "lower" and "upper" can encompass both orientations. The device can be otherwise oriented (rotated 90 degrees or located in any other orientation) and the spatially relative descriptors used herein interpreted accordingly.

[0046] Moreover, terms such as "first" and "second" are used herein only to distinguish one from another of a same name, and do not necessarily require or imply any such actual relationship or order between or among the referenced items.

[0047] It should be noted that the above-mentioned embodiments illustrate rather than limit the application, and that those skilled in the art will be able to design many alternative embodiments without departing from the scope of the application. Accordingly, the legal scope of the application is defined only by the appended claims.

Claims

1. A precise grafting method based on vascular bundle configuration, characterized in that, Includes the following steps: Step 1: Obtain microscopic images of the rootstock cross-section and identify the vascular bundle configuration features of the rootstock cross-section; Step 2: Obtain microscopic images of the scion cross section and identify the vascular bundle configuration features of the scion cross section; Step 3: Overlay the microscopic images of the rootstock section with the microscopic images of the scion section, and use image analysis technology to pre-match the vascular bundle configuration of the scion and rootstock with the goal of maximizing the overlap area of ​​the vascular bundle configuration features of the rootstock and scion. Step 4: After the pre-matching is completed, in this pre-matching state, mark the pose representation data in the cross-sectional microscopic images of the scion and rootstock at this time, so as to provide guidance for the subsequent actual grafting work of the scion and rootstock; Step 5: Transform the pose representation data marked in Step 4 into the coordinate system of the grafting device, thereby guiding the grafting device to adjust the pose of the scion and / or rootstock, and complete the docking of the scion and rootstock to maximize the overlap area of ​​the vascular bundles of the scion and rootstock. Step 6: After docking, secure the docking points.

2. The precise grafting method based on vascular bundle configuration according to claim 1, characterized in that: The vascular bundle configuration features of the rootstock cross section include characteristic data on the vascular bundle profile and distribution.

3. The precise grafting method based on vascular bundle configuration according to claim 1, characterized in that: The vascular bundle configuration features of the scion section include characteristic data on the vascular bundle outline and distribution.

4. The precise grafting method based on vascular bundle configuration according to claim 1, characterized in that: During pre-matching, it is necessary to ensure that the overlapping area of ​​the vascular bundle configuration features between the rootstock and the scion accounts for more than 70% of the total vascular bundle area of ​​the rootstock cross-section. If this requirement cannot be met, the subsequent grafting work should not be carried out, and the scion or rootstock seedling should be replaced before pre-matching.

5. The precise grafting method based on vascular bundle configuration according to claim 1, characterized in that: Pose characterization data in the labeled scion and rootstock cross-sectional micrographs include: the center of the elliptical cross-section of the scion and rootstock, the direction of the major axis, and the position of the intersection of the two major axes of the scion and rootstock in the rootstock cross-sectional micrograph.

6. The precise grafting method based on vascular bundle configuration according to claim 1, characterized in that: Use flexible grafting clips or spray-apply fast-curing adhesive to fix the joint.

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