Embryo pipetting device

Abstract

The embryo pipetting device comprises a Pasteur pipette made of glass, a base, a control piece and a connecting pipe arranged at the tail of the Pasteur pipette in a sleeving mode, a piston pipe made of a hard material is arranged at the end, away from the tail of the Pasteur pipette, of the connecting pipe, and a piston is arranged in the piston pipe; the piston comprises a sealing part which is attached to the pipe wall of the piston pipe and is made of an elastic material, the piston pipe is fixed to the base, the control piece comprises a pedal plate for controlling the piston to move in the piston pipe, and compared with a combination of a rubber head and a Pasteur pipette, the wrist leans against a table top in the operation process, so that the Pasteur pipette can be kept stable in the whole liquid suction process; compared with a combination of a mouth suction tube and a Pasteur suction tube, the suction amount is convenient to control, the fineness is higher, and pollution to a laboratory is avoided.

Description

Technical Field

[0001] This invention belongs to the field of assisted reproductive technology, specifically relating to an embryo transfer device. Background Technology

[0002] In assisted reproductive technology laboratories, pipettes are used to transfer embryos and perform other procedures. Pipettes include Bartholin's pipettes. A Bartholin's pipette is a glass tube with two ends, one end tapered to form the head and the other end forming the tail.

[0003] The embryos are first cultured in microdroplets in plastic culture dishes. Generally, each microdroplet contains 25 μL of liquid, with a liquid level of about 3 mm. The egg size is about 0.15 mm. Scratching the culture dish may release plastic components that are harmful to the embryo. In order to avoid the tip of the Pasteur pipette touching the plastic culture dish and scratching it, the transfer operation requires a very high degree of precision from the operator, who must not tremble under the microscope.

[0004] There are currently two commonly used operating methods:

[0005] 1. A pipette consists of a Pasteur pipette and a rubber tip at the end of the Pasteur pipette. During operation, the operator stabilizes the pipette by resting their elbow against the work surface and uses their thumb and forefinger to press the tip to draw liquid. This method is simple, straightforward, and convenient. However, it demands a high degree of dexterity and stability from the operator.

[0006] 2. Pipettes include glass Pasteur pipettes and oral pipettes. One end of the oral pipette is connected to the Pasteur pipette, followed by an air filter, and then an extension tube. A disposable tip can be inserted into the end of the extension tube before the pipette is held in the mouth for manipulation. This method allows for wrist support on the work surface, providing greater stability and reducing the difficulty of operation. However, the oral pipette is longer; the precision required for pipetting is between 1-2 μL, which is difficult to achieve with oral pipetting; and oral pipetting prevents masks from being worn tightly, potentially causing laboratory contamination. Summary of the Invention

[0007] In order to overcome the shortcomings of the prior art, the present invention discloses an embryo transfer device that combines the advantages of the two operating methods in the prior art and overcomes their disadvantages.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: an embryo transfer device, comprising a Bartholin's pipette made of glass, a base, a control component, and a connecting tube sleeved at the tail end of the Bartholin's pipette. The end of the connecting tube away from the tail end of the Bartholin's pipette is provided with a piston tube made of rigid material. A piston is provided inside the piston tube. The piston includes a sealing part made of elastic material that fits against the wall of the piston tube. The piston tube is fixed on the base. The control component includes a foot pedal for controlling the movement of the piston inside the piston tube.

[0009] Using the above solution, the connecting tube and piston tube can be integrated as a single unit or detachably connected. The connecting tube can be a flexible tube or a combination of a rigid tube and a flexible tube. Preferably, the piston tube is made of a transparent rigid material, such as acrylic, PC, or UPVC, for easy observation of the piston's condition. Preferably, the base has anti-slip strips on the ground-facing surface.

[0010] During operation, the operator rests their wrist on the table to control the head of the Pasteur pipette to the aspiration point of the petri dish, and then applies force to the foot pedal to control the piston movement, drawing the liquid in the petri dish into the Pasteur pipette.

[0011] Compared to the combination of a rubber tip and a Pasteur pipette, this invention allows the wrist to rest on the work surface during operation, which keeps the Pasteur pipette stable throughout the liquid aspiration process. Compared to the combination of an oral pipette and a Pasteur pipette, this invention makes it easier to control the aspiration volume, provides higher precision, and does not cause laboratory contamination.

[0012] As a further feature of the present invention, the control component also includes a large gear and a small gear, both of which are rotatably connected to the base. The outer diameter of the large gear is larger than that of the small gear, and the outer edges of the large gear and the small gear mesh with each other. The piston includes a push-pull rod, and the sealing part is linked to the large gear through the push-pull rod. The small gear is provided with a fixing rod that is linked to the foot pedal. The fixing rod is coaxially arranged with the small gear and fixedly connected to the small gear.

[0013] By using the above scheme, the piston moves slowly and uniformly inside the piston tube through the cooperation between the large gear and the small gear, which can control the precision of embryo transfer operation to 1-2 μL.

[0014] As a further feature of the present invention, the large gear is provided with an eccentric shaft, the axis of the eccentric shaft is parallel to the axis of the large gear, the two ends of the push-pull rod are respectively rotatably connected to the sealing part and the eccentric shaft, one end of the foot pedal is fixedly connected to the fixing rod, and the foot pedal includes a reset member that keeps the end of the foot pedal away from the fixing rod in a tilted state when no external force is applied.

[0015] Using the above solution, the "tilted state" refers to the situation where, in actual use, the end of the foot pedal furthest from the fixed rod is higher than the end of the foot pedal closest to the fixed rod.

[0016] During operation, the operator rests their wrist on the work surface, controls the tip of the Pasteur pipette to the aspiration point of the petri dish, and then applies force to the end of the foot pedal away from the fixing rod. This causes the fixing rod to rotate the pinion, which in turn rotates the large gear. As the large gear rotates, the push-pull rod moves the sealing part, thus achieving aspiration. After the operator reduces or removes the force applied to the foot pedal, a reset mechanism causes the foot pedal to rotate in the opposite direction, which in turn causes the fixing rod to rotate the pinion in the opposite direction, and the push-pull rod moves the sealing part back to its initial position. The reset mechanism can be a compression spring located between the foot pedal and the base.

[0017] As a further feature of the present invention, the side wall of the push-pull rod is provided with a rack that meshes with the large gear, and the fixed rod and the foot pedal are linked by a linkage.

[0018] Using the above method, after the operator applies force to the foot pedal, the fixed rod drives the small gear to rotate through the linkage, and the small gear drives the large gear to rotate, thus moving the piston.

[0019] As a further feature of the present invention, the side wall of the piston rod is provided with a notch for the large gear to be inserted, the large gear and the rack mesh through the notch, and the push-pull rod is clearance-fitted with the piston.

[0020] The above-described design allows the large gear to better control the piston's movement, while also making the overall design more compact and reducing the footprint of the invention. Preferably, the piston section is a circular tube, and the push-pull rod is a circular rod.

[0021] As a further feature of the present invention, the linkage includes a linkage rod and a connecting rod. The axis of the linkage rod is perpendicular to the axis of the fixed rod. A fixing part is provided between the linkage rod and the fixed rod to keep the linkage rod and the fixed rod relatively fixed in the circumferential direction of the fixed rod. The connecting rod is provided at the end of the linkage rod away from the fixing part and is parallel to the fixed rod. The connecting rod passes through the foot pedal and is rotatably connected to the foot pedal.

[0022] Using the above method, during operation, the operator rests their wrist on the work surface, controlling the tip of the Pasteur pipette to the aspiration point of the petri dish. Then, force is applied to the foot pedal, causing it to rotate the connecting rod and the fixing rod along the axis of the fixing rod. This, in turn, rotates the pinion gear. Through the linkage between the pinion and the large gear, the large gear drives the piston to move linearly within the piston tube, thus drawing liquid from the petri dish into the Pasteur pipette. To return the piston to its original position, simply apply a force in the opposite direction to the foot pedal, causing the connecting rod and the fixing rod to rotate in the opposite direction.

[0023] As a further feature of the present invention, the linkage includes a motor capable of driving the fixed rod to rotate, the foot pedal includes pedal one, pedal two and a reset member, pedal one and pedal two are rotatably connected, the reset member keeps pedal one and pedal two at an angle greater than 10° and less than 90° when no external force is applied, the motor includes a switch disposed on pedal one and / or pedal two, the switch is triggered when the angle between pedal one and pedal two decreases.

[0024] Using the above solution, operator control of force is unnecessary, making operation more convenient. The foot pedal can be separate from the base, or it can be integrated with the base. Preferably, the switch is continuously triggered when pedal one and pedal two are in contact. The preferred switch triggering logic is alternating triggering: when the switch is triggered and held for the first time, the motor drives the fixed rod to rotate until the switch disengages from the triggered state. When the switch is triggered and held for the second time, the motor drives the fixed rod to rotate in the opposite direction. The number of rotations of the fixed rod in the reverse direction matches the number of rotations during the first triggering. Even if the switch remains triggered, the motor automatically stops. The first and second triggers alternate, facilitating operator control.

[0025] During operation, the operator rests their wrist on the work surface, controls the tip of the Pasteur pipette to the aspiration point of the petri dish, and then applies force to pedal one, moving it towards pedal two, triggering the switch and maintaining the triggered state. The motor starts operating, causing the fixed rod to rotate, moving the piston within the piston tube. After aspiration is complete, the operator releases the force applied to pedal one, disengaging the switch from the triggered state, stopping the motor, and pedal one returns to its original position under the action of the reset mechanism. After all operations are completed, force is applied to pedal one again, triggering the switch and maintaining the triggered state, moving the piston back to its initial position. The reset mechanism can be a torsion spring located at the connection between pedal one and pedal two, or an electric telescopic rod connected to pedal one. The switch can be a push-button switch or pressure switch located on pedal one and / or pedal two, or a contact point located on pedal one and pedal two.

[0026] Depending on the operator's habits, the switch can also be designed so that when the switch is triggered, the motor drives the fixed rod to rotate, and when the switch is disconnected, the motor automatically rotates the fixed rod back to the initial position. In this setting, the number of rotations of the fixed rod after the switch is triggered is preset, and the motor stops after the fixed rod has rotated to the predetermined number of rotations. This setting can reduce the operator's operation steps and prevent the experiment from being affected by forgetting to reset, but it does not allow for free control of the number of rotations of the fixed rod.

[0027] Preferably, the second pedal and the base are separate units. The second pedal has a slot for placing the motor. The second pedal and the base are connected by a plug or a snap-fit. The operator can choose to control the rotation of the fixed rod with a motor or a linkage rod, depending on the actual usage. Motor control is convenient, labor-saving, and highly accurate, but it requires a power supply and has certain requirements for the usage site.

[0028] As a further feature of the present invention, the base is provided with a fixing groove, the bottom of the fixing groove is provided with an embedding groove for the piston to be embedded and fixed, the large gear and the small gear are both located in the fixing groove, the fixing rod passes through the fixing groove and rotates with the fixing groove, and the foot pedal and the linkage are provided outside the fixing groove.

[0029] Using the above solution, the embedded groove is used to fix the piston tube and prevent the piston tube from moving. The fixing groove also prevents the operator from touching the large gear and small gear when operating the foot pedal.

[0030] As a further feature of the present invention, the piston tube includes a sleeve portion for mounting a connecting tube and a piston portion for piston movement. The outer diameter of the sleeve portion gradually decreases from the side closer to the piston portion to the side farther from the piston portion. The inner diameters of the sleeve portion and the piston portion are the same. The maximum outer diameter of the sleeve portion is equal to the outer diameter of the piston portion. The connecting tube includes an elastic end fitted onto the sleeve portion. A fixing sleeve is fitted at the connection between the piston portion and the sleeve portion. The fixing sleeve is interference-fitted with the elastic end on the inner wall of the sleeve portion and threadedly fitted with the outer wall of the piston portion on the inner wall of the fixing sleeve.

[0031] When using the above method, to fix the connecting pipe and piston pipe, first, the fixing sleeve is placed on the connecting pipe, then the elastic end is placed on the sleeve part, and then the fixing sleeve is moved towards the piston part until the fixing sleeve and piston part are threadedly engaged. The shape of the outer wall of the sleeve part facilitates the fitting and removal of the elastic end. The threaded engagement between the fixing sleeve and the piston part facilitates assembly and disassembly. Preferably, the inner wall of the fixing sleeve that abuts against the elastic end has the same shape as the outer wall of the sleeve part, and preferably the maximum diameter of the outer wall of the sleeve part is 1.1 to 1.2 times the minimum diameter.

[0032] As a further feature of the present invention, the connecting tube includes a first flexible tube, a second flexible tube, and a rigid tube. Both the first flexible tube and the second flexible tube are elastic. The elastic end is located at the end of the first flexible tube away from the rigid tube. The rigid tube includes a first fixed end for fitting and fixing the first flexible tube and a second fixed end for fitting and fixing the second flexible tube. An air filter is provided inside the rigid tube. The end of the second flexible tube away from the rigid tube is fitted onto the Bass straw.

[0033] Using the above method, dust may enter the piston tube. The air filter ensures that no external contaminants enter the Pasteur suction tube during the operator's extraction process. After the air filter's performance deteriorates, the rigid tube can be removed and replaced, or the rigid tube can be removed, cleaned, and reinstalled to maintain the air filter's filtration effect. The presence of flexible hose two facilitates the operator's replacement of the Pasteur suction tube.

[0034] The present invention will now be further described with reference to the accompanying drawings. Attached Figure Description

[0035] Appendix Figure 1 This is a schematic diagram of a specific embodiment of the present invention;

[0036] Appendix Figure 2 This is a top view of the base in a specific embodiment of the present invention;

[0037] Appendix Figure 3 For the appendix Figure 2 AA section view;

[0038] Appendix Figure 4 This is a schematic diagram of the connection between the Pasteur straw and the connecting tube in a specific embodiment of the present invention;

[0039] Appendix Figure 5 This is a schematic diagram of the connection between the piston tube and the connecting tube in a specific embodiment of the present invention;

[0040] Appendix Figure 6 This is a schematic diagram of the base in a specific embodiment two of the present invention;

[0041] Appendix Figure 7 This is a side view of the base in a specific embodiment two of the present invention;

[0042] Appendix Figure 8 For the appendix Figure 7 BB section view;

[0043] Appendix Figure 9 For the appendix Figure 7 CC section view;

[0044] Appendix Figure 10 This is a schematic diagram of the overall structure of a specific embodiment of the present invention;

[0045] Appendix Figure 11 This is a top view of the base in a specific embodiment three of the present invention;

[0046] Appendix Figure 12 For the appendix Figure 11 DD sectional view.

[0047] Example 1 and Example 2: Base 1, Fixing groove 11, Embedding groove 12, Foot pedal 2, Pedal 1 21b, Pedal 2 22b, Placement groove 221b, Reset piece 23b, Large gear 3, Small gear 4, Fixing rod 41, Protrusion 411, Linking piece 5, Linking rod 51a, Groove 511a, Connecting rod 52a, Motor 53b, Switch 531b, Bass straw 6, Connecting tube 7, Hose 1 71, Hose 2 72, Rigid tube 73, Fixing end 1 731, Fixing end 2 732, Air filter 733, Piston tube 8, Notch 81, Sleeve part 82, Piston part 83, Fixing sleeve 84, Piston 9, Sealing part 91, Push-pull rod 92.

[0048] Example 3: Base 1c, foot pedal 2c, reset component 21c, large gear 3c, eccentric shaft 31c, small gear 4c, fixing rod 41c, Bass straw 5c, connecting tube 6c, piston tube 7c, piston 8c, sealing part 81c, push-pull rod 9c. Detailed Implementation

[0049] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0050] In the description of this invention, it should be noted that, unless otherwise specified, the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are used only for the convenience of describing the invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0051] Specific embodiments of the present invention, for example Figure 1-12 As shown.

[0052] Example 1: As shown in the attached document Figures 1-5As shown, an embryo transfer device includes a base 1, a foot pedal 2, a large gear 3, a small gear 4, a linkage 5, a glass Pasteur pipette 6, and a connecting tube 7 sleeved at the tail of the Pasteur pipette 6. A piston tube 8 made of rigid material is provided at one end of the connecting tube 7 away from the tail of the Pasteur pipette 6. A piston 9 is provided inside the piston tube 8, and the piston 9 includes a sealing part 91 made of elastic material that fits against the wall of the piston tube 8. The piston tube 8 is fixed to the base 1, and both the large gear 3 and the small gear 4 are rotatably connected to the base 1. The outer diameter of the large gear 3 is larger than that of the small gear 4, and the outer edges of the large gear 3 and the small gear 4 mesh with each other. The piston 9 includes a push-pull rod 92, which is clearance-fitted with the piston part 83. The side wall of the push-pull rod 92 is provided with a rack, and the side wall of the piston tube 8 is provided with a notch 81. The large gear 3 and the rack mesh with each other through the notch 81. The small gear 4 is provided with a fixed rod 41 that is coaxially arranged with and fixedly connected to the small gear 4. The fixed rod 41 and the foot pedal 2 are linked by a linkage 5.

[0053] The base 1 has a fixing groove 11, and the bottom of the fixing groove 11 has an embedding groove 12 for partially embedding and fixing the piston tube 8. The large gear 3 and the small gear 4 are both located in the fixing groove 11. The fixing rod 41 passes through the fixing groove 11 and rotates with the fixing groove 11. The foot pedal 2 and the linkage 5 are located outside the fixing groove 11. The embedding groove 12 is used to fix the piston tube 8 and prevent the piston tube 8 from moving. The fixing groove 11 prevents the operator from touching the large gear 3 and the small gear 4 when operating the foot pedal 2.

[0054] The linkage 5 consists of a linkage rod 51a and a connecting rod 52a. The axis of the linkage rod 51a is perpendicular to the axis of the fixed rod 41. In this embodiment, the fixed rod 41 is provided with a protrusion 411. The side edge of the protrusion 411 is parallel to the axis of the fixed rod 41. The linkage rod 51a is provided with a groove 511a for the protrusion 411 to be inserted. The extension direction of the groove 511a is perpendicular to the axis of the linkage rod 51a. The protrusion 411 is in the shape of a right hexagonal prism. The shape of the groove 511a is the same as the shape of the protrusion 411. The protrusion 411 can also be set as a right triangular prism, a right quadrangular prism or a right pentagonal prism according to the requirements. The cooperation between the protrusion 411 and the groove 511a keeps the linkage rod 51a and the fixed rod 41 relatively fixed in the circumference of the fixed rod 41. The connecting rod 52a is located at the end of the connecting rod 51a away from the fixed part and is parallel to the fixed rod 41. The connecting rod 52a passes through the foot pedal 2 and is rotatably connected to the foot pedal 2.

[0055] During operation, the operator rests their wrist on the work surface, controlling the tip of the Pasteur pipette 6 to the aspiration point of the petri dish. Then, force is applied to the foot pedal 2, causing it to rotate the connecting rod 51a and the fixed rod 41 along the axis of the fixed rod 41. This, in turn, rotates the pinion 4. Through the linkage between the pinion 4 and the large gear 3, the large gear 3 drives the piston 9 to move linearly within the piston tube 8, thus drawing liquid from the petri dish into the Pasteur pipette 6. To reset the piston 9, simply apply a force in the opposite direction to the foot pedal 2, causing the connecting rod 51a and the fixed rod 41 to rotate in the opposite direction.

[0056] Through the cooperation between the large gear 3 and the small gear 4, the piston 9 moves slowly and uniformly inside the piston tube 8, which can control the precision of embryo transfer operation within 1-2 μL.

[0057] When not in use, the linkage 51a can be removed for easy storage and subsequent maintenance.

[0058] In this embodiment, the piston tube 8 is made of a transparent, rigid material, such as acrylic, PC, or UPVC, to facilitate observation of the piston 9. In this embodiment, the base 1 has anti-slip strips on its ground-facing surface. In this embodiment, the piston part 83 is a round tube, and the push-pull rod 92 is a round rod.

[0059] Compared to the combination of a rubber tip and a Pasteur pipette 6, the present invention allows the wrist to rest on the table during operation, which keeps the Pasteur pipette 6 stable throughout the liquid aspiration process. Compared to the combination of an oral pipette and a Pasteur pipette 6, the present invention makes it easier to control the aspiration volume, provides higher precision, and does not cause laboratory contamination.

[0060] The connecting tube 7 includes a first flexible tube 71, a second flexible tube 72, and a rigid tube 73. Both the first flexible tube 71 and the second flexible tube 72 are elastic. The rigid tube 73 includes a first fixed end 731 for attaching and securing the first flexible tube 71 and a second fixed end 732 for attaching and securing the second flexible tube 72. An air filter 733 is installed inside the rigid tube 73. The end of the second flexible tube 72 furthest from the rigid tube 73 is attached to the Pasteur straw 6. Dust may enter the piston tube 8; the air filter 733 ensures that no external contaminants enter the Pasteur straw 6 during extraction. When the performance of the air filter 733 deteriorates, the rigid tube 73 can be removed and replaced, or it can be removed, cleaned, and reinstalled to maintain its filtering effect. The presence of the second flexible tube 72 facilitates the replacement of the Pasteur straw 6 by the operator.

[0061] The piston tube 8 includes a sleeve portion 82 for the connecting tube 7 to be fitted and a piston portion 83 for the piston 9 to move. The outer diameter of the sleeve portion 82 gradually decreases from the side closer to the piston portion 83 to the side farther away from the piston portion 83. The inner diameters of the sleeve portion 82 and the piston portion 83 are the same. The maximum outer diameter of the sleeve portion 82 is equal to the outer diameter of the piston portion 83. The hose 71 includes an elastic end fitted on the sleeve portion 82. A fixing sleeve 84 is fitted at the connection between the piston portion 83 and the sleeve portion 82. The fixing sleeve 84 is interference-fitted with the elastic end on the inner wall of the sleeve portion 82 and threadedly fitted with the outer wall of the piston portion 83 on the inner wall of the fixing sleeve 84.

[0062] When it is necessary to fix the connecting pipe 7 and the piston pipe 8, first, the fixing sleeve 84 is fitted onto the connecting pipe 7, then the elastic end is fitted onto the fitting part 82, and then the fixing sleeve 84 is moved toward the piston part 83 until the fixing sleeve 84 and the piston part 83 are threadedly engaged. The shape of the outer wall of the fitting part 82 facilitates the fitting and removal of the elastic end. The threaded engagement between the fixing sleeve 84 and the piston part 83 facilitates disassembly and assembly. In this embodiment, the inner wall of the fixing sleeve 84 that abuts against the elastic end has the same shape as the outer wall of the fitting part 82, and the maximum diameter of the outer wall of the fitting part 82 is 1.2 times the minimum diameter.

[0063] Example 2: As shown in the attached document Figures 6-9 As shown, Embodiment 2 is largely the same as Embodiment 1, except that the linkage 5 in Embodiment 2 is a motor 53b that can drive the fixed rod 41 to rotate, and the foot pedal 2 includes pedal 1 21b, pedal 2 22b and reset component 23b.

[0064] In this embodiment, the motor 53b includes a rotating shaft with a slot for inserting a protrusion 411. Through the cooperation of the protrusion 411 and the slot, the rotating shaft drives the fixed rod 41 to rotate. The pedal 1 21b and the pedal 2 22b are rotatably connected. The reset member 23b keeps the pedal 1 21b and the pedal 2 22b at a 20° angle when there is no external force. The motor 53b includes a switch 531b disposed on the pedal 2 22b. The switch 531b is triggered when the angle between the pedal 1 21b and the pedal 2 22b decreases.

[0065] Compared to the linkage 5 in Embodiment 1, the linkage 5 in this embodiment does not require operator control of force, making operation more convenient. In this embodiment, switch 531b is triggered when pedal one 21b and pedal two 22b are in contact. In this embodiment, the triggering logic of switch 531b is alternating triggering. That is, when switch 531b is triggered for the first time and held in the triggered state, motor 53b drives fixed rod 41 to rotate until switch 531b is released from the triggered state. When switch 531b is triggered for the second time and held in the triggered state, motor 53b drives fixed rod 41 to rotate in the opposite direction. After the number of rotations of fixed rod 41 in the opposite direction is the same as the number of rotations of fixed rod 41 when switch 531b is triggered for the first time, even if switch 531b remains in the triggered state, motor 53b will still stop automatically. The first and second triggers alternate, which is convenient for operator control.

[0066] During operation, the operator rests their wrist on the work surface, controls the head of the Pasteur pipette 6 to the aspiration area of ​​the culture dish, and then applies force to pedal 21b, causing pedal 21b to move towards pedal 22b, triggering switch 531b and maintaining the triggered state. Motor 53b starts operating, causing the fixing rod 41 to rotate, and the piston 9 to move within the piston tube 8. After aspiration is completed, the operator releases the force applied to pedal 21b, causing switch 531b to disengage from the triggered state, motor 53b stops, and pedal 21b returns to its original position under the action of reset element 23b. After all operations are completed, force is applied to pedal 21b again, triggering switch 531b and maintaining the triggered state, causing piston 9 to move to the initial position. In this embodiment, reset element 23b is a torsion spring located at the connection between pedal 21b and pedal 22b. Switch 531b is a push-button switch 531b located on pedal 22b.

[0067] In this embodiment, the second pedal 22b and the base 1 are separately set. The second pedal 22b is provided with a placement slot 221b for placing the power supply 53b. The second pedal 22b and the base 1 are plugged into each other.

[0068] Example 3: As shown in the attached document Figures 10-12 As shown, an embryo transfer device includes a base 1c, a foot pedal 2c, a large gear 3c, a small gear 4c, a glass Pasteur pipette 5c, and a connecting tube 6c sleeved at the tail of the Pasteur pipette 5c. A piston tube 7c made of rigid material is provided at one end of the connecting tube 6c away from the tail of the Pasteur pipette 5c. A piston 8c is provided inside the piston tube 7c. The piston 8c includes a sealing part 81c made of elastic material that fits against the wall of the piston tube 7c. The piston tube 7c is fixed on the base 1c. The large gear 3c and the small gear 4c are rotatably connected to the base 1c. The outer diameter of the large gear 3c is larger than the outer diameter of the small gear 4c, and the outer edges of the large gear 3c and the small gear 4c mesh with each other.

[0069] The large gear 3c is provided with an eccentric shaft 31c, which is parallel to the axis of the large gear 3c. The piston 8c includes a push-pull rod 9c. The sealing part 81c is connected to the large gear 3c through the push-pull rod 9c. The two ends of the push-pull rod 9c are rotatably connected to the piston 8c and the eccentric shaft 31c, respectively. The small gear 4c is provided with a fixing rod 41c that is coaxial with and fixedly connected to the small gear 4c. One end of the foot pedal 2c is fixedly connected to the fixing rod 41c. The foot pedal 2c includes a reset member 21c that keeps the end of the foot pedal 2c away from the fixing rod 41c in a tilted state when there is no external force. The tilted state means that in actual use, the height of the end of the foot pedal 2c away from the fixing rod 41c from the ground is greater than the height of the end of the foot pedal 2c close to the fixing rod 41c from the ground.

[0070] By coordinating the large gear 3c and the small gear 4c, the piston 8c moves slowly and uniformly within the piston tube 7c, which allows the precision of embryo transfer to be controlled within 1-2 μL.

[0071] During operation, the operator rests their wrist on the table and controls the tip of the Pasteur pipette 5c to the aspiration point of the petri dish. Force is then applied to the end of the foot pedal 2c furthest from the fixing rod 41c, causing the fixing rod 41c to rotate the pinion 4c, which in turn rotates the gear 3c. During the rotation of the gear 3c, the push-pull rod 9c moves the sealing part 81c, achieving aspiration. After operation, the operator reduces or removes the force applied to the foot pedal 2c. The reset element 21c causes the foot pedal 2c to rotate in the opposite direction, causing the fixing rod 41c to rotate the pinion 4c in the opposite direction, and the push-pull rod 9c to move the sealing part 81c back to its initial position. In this embodiment, the reset element 21c is a torsion spring located between the foot pedal 2c and the base 1c.

[0072] In this embodiment, the piston tube 7c is made of a transparent rigid material, such as acrylic, PC, or UPVC, to facilitate observation of the piston 8c. Preferably, the base 1c has anti-slip strips on the surface facing the ground.

[0073] Compared to the combination of a rubber tip and a Pasteur pipette 5c, this invention allows the wrist to rest on the work surface during operation, which keeps the Pasteur pipette 5c stable throughout the liquid aspiration process. Compared to the combination of an oral pipette and a Pasteur pipette 5c, this invention makes it easier to control the aspiration volume, provides higher precision, and does not cause laboratory contamination.

[0074] This invention is not limited to the specific embodiments described above. Those skilled in the art can implement this invention using various other specific embodiments based on the content disclosed herein. Any simple changes or modifications made to the design structure and concept of this invention fall within the protection scope of this invention.

Claims

1. An embryo transfer device comprising a glass Pasteur pipette, characterized in that: It also includes a base, a control unit, and a connecting tube fitted onto the tail of the Bass straw. The end of the connecting tube away from the tail of the Bass straw is provided with a piston tube made of a rigid material. A piston is provided inside the piston tube. The piston includes a sealing part made of an elastic material that fits against the wall of the piston tube. The piston tube is fixed on the base. The control unit includes a foot pedal for controlling the movement of the piston inside the piston tube.

2. The embryo transfer device according to claim 1, characterized in that: The control component also includes a large gear and a small gear, both of which are rotatably connected to the base. The outer diameter of the large gear is larger than that of the small gear, and the outer edges of the large gear and the small gear mesh with each other. The piston includes a push-pull rod, and the sealing part is linked to the large gear through the push-pull rod. The small gear is provided with a fixing rod that is linked to the foot pedal. The fixing rod is coaxially arranged with the small gear and fixedly connected to the small gear.

3. The embryo transfer device according to claim 2, characterized in that: The large gear is provided with an eccentric shaft, the axis of which is parallel to the axis of the large gear. The two ends of the push-pull rod are rotatably connected to the sealing part and the eccentric shaft, respectively. One end of the foot pedal is fixedly connected to the fixing rod. The foot pedal includes a reset component that keeps the end of the foot pedal away from the fixing rod in a tilted state when no external force is applied.

4. The embryo transfer device according to claim 2, characterized in that: The push-pull rod has a rack on its side wall that meshes with the large gear, and the fixed rod and the foot pedal are connected by a linkage.

5. The embryo transfer device according to claim 4, characterized in that: The piston rod has a notch on its side wall for the large gear to be inserted. The large gear and the rack mesh through the notch, and the push-pull rod is clearance-fitted with the piston.

6. The embryo transfer device according to claim 5, characterized in that: The linkage includes a linkage rod and a connecting rod. The axis of the linkage rod is perpendicular to the axis of the fixed rod. A fixing part is provided between the linkage rod and the fixed rod to keep the linkage rod and the fixed rod relatively fixed in the circumferential direction of the fixed rod. The connecting rod is located at the end of the linkage rod away from the fixing part and is parallel to the fixed rod. The connecting rod passes through the foot pedal and is rotatably connected to the foot pedal.

7. The embryo transfer device according to claim 5, characterized in that: The linkage includes a motor that can drive the fixed rod to rotate. The foot pedal includes pedal one, pedal two, and a reset component. Pedal one and pedal two are rotatably connected. The reset component keeps pedal one and pedal two at an angle greater than 10° and less than 90° when no external force is applied. The motor includes a switch installed on pedal one and / or pedal two. The switch is triggered when the angle between pedal one and pedal two decreases.

8. An embryo transfer device according to claim 3, 6, or 7, characterized in that: The base is provided with a fixing groove, and the bottom of the fixing groove is provided with an embedding groove for the piston to be embedded and fixed. The large gear and the small gear are both located in the fixing groove. The fixing rod passes through the fixing groove and rotates with the fixing groove. The foot pedal and the linkage are located outside the fixing groove.

9. The embryo transfer device according to claim 8, characterized in that: The piston tube includes a sleeve portion for mounting a connecting tube and a piston portion for piston movement. The outer diameter of the sleeve portion gradually decreases from the side closer to the piston portion to the side farther away from the piston portion. The inner diameters of the sleeve portion and the piston portion are the same. The maximum outer diameter of the sleeve portion is equal to the outer diameter of the piston portion. The connecting tube includes an elastic end fitted onto the sleeve portion. A fixing sleeve is fitted at the connection between the piston portion and the sleeve portion. The fixing sleeve is interference-fitted with the elastic end on the inner wall of the sleeve portion and threadedly fitted with the outer wall of the piston portion on the inner wall of the fixing sleeve.

10. An embryo transfer device according to claim 3, 6, 7, or 9, characterized in that: The connecting tube includes a first flexible tube, a second flexible tube, and a rigid tube. Both the first flexible tube and the second flexible tube are elastic. The elastic end is located at the end of the first flexible tube away from the rigid tube. The rigid tube includes a first fixed end for fitting and fixing the first flexible tube and a second fixed end for fitting and fixing the second flexible tube. An air filter is provided inside the rigid tube. The end of the second flexible tube away from the rigid tube is fitted onto the Bass straw.

Images