A strain of Pediococcus pentosaceus and its application in promoting plant growth
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SICHUAN ZHONGNONG RUNZE BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-12-29
- Publication Date
- 2026-06-30
Smart Images
Figure CN121450545B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of microbial technology, specifically to a strain of Pediococcus pentosaceus and its application in promoting plant growth. Background Technology
[0002] Traditional chemical pesticides and fertilizers are important inputs in current agricultural production. Although they can directly increase crop yields by controlling pests and diseases, long-term and excessive use can easily cause multiple problems: First, they damage the soil structure, leading to soil compaction, reduced porosity, and consequently, a decline in soil fertility; second, pesticide residues can easily seep into water bodies and soil or diffuse through the atmosphere, causing environmental pollution, which contradicts the concept of sustainable development in green agriculture.
[0003] With the increasing demands for ecological protection and long-term benefits in modern agriculture, reducing the application of chemical agricultural inputs has become an industry consensus. Against this backdrop, microbial preparations, due to their advantages such as environmental friendliness, sustainability, improvement of soil microecology, and promotion of plant nutrient absorption, have become an important direction for promoting sustainable agricultural development. Lactic acid bacteria, as a common type of probiotic, have traditionally been used primarily in food fermentation and animal feed. In recent years, studies have found that some lactic acid bacteria, such as certain strains of *Lactobacillus* and *Pediococcus*, can successfully colonize the rhizosphere of plants and promote plant growth through various mechanisms, such as producing plant growth hormones (e.g., indole-3-acetic acid IAA), dissolving insoluble phosphates, producing siderophores, and inducing systemic resistance in plants. However, these lactic acid bacteria have significant limitations in practical agricultural applications: poor stress resistance, difficulty adapting to complex soil environments; growth-promoting effects greatly affected by environmental factors, resulting in insufficient stability; and a narrow range of applicable crops, making widespread promotion difficult. Therefore, screening lactic acid bacteria growth-promoting strains that combine high efficiency and stability has significant practical and theoretical value for overcoming current technological bottlenecks and promoting the development of green agriculture.
[0004] In view of this, this invention is hereby proposed. Summary of the Invention
[0005] Based on the above problems, the purpose of this invention is to provide a strain of Pediococcus pentosaceus and its application. This strain has strong stress resistance, strong adaptability to soil environment, and can effectively promote the growth of various plants.
[0006] To achieve the above objectives, the first technical solution of the present invention provides a strain of *Pediococcus pentosaceus* ZNRZ-B053, which is deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 3, 2025, with accession number CGMCC NO.36457, and is classified as *Pediococcus pentosaceus*. Pediococcuspentosaceus .
[0007] Preferably, the strain is isolated from kimchi brine.
[0008] The second technical solution of the present invention provides a microbial inoculant containing Pediococcus pentosaceus ZNRZ-B053.
[0009] Preferably, the viable count of Pediococcus pentosaceus ZNRZ-B053 in the microbial agent is ≥10. 5 CFU / mL or ≥10 5 CFU / g. In addition to Pediococcus pentosaceus ZNRZ-B053, the microbial agent may also contain some buffers or stabilizers to stabilize the pH of the system, protect the activity of live bacteria, and improve the storage and transportation performance of the agent.
[0010] Preferably, the microbial agent is in liquid, semi-liquid, or solid form.
[0011] The third technical solution of the present invention provides the application of Pediococcus pentosaceus ZNRZ-B053 or microbial agents in soil conditioning. Pediococcus pentosaceus ZNRZ-B053 or microbial agents, through the secretion of substances such as lactic acid and citric acid, can convert insoluble phosphorus and potassium in the soil into readily available phosphorus and potassium that can be absorbed by plants, exhibiting good phosphorus and potassium solubilization capabilities.
[0012] The fourth technical solution of the present invention provides the application of the Pentosacchariphyte ZNRZ-B053 or microbial inoculants in the prevention and control of plant diseases.
[0013] Preferably, the plant diseases include fungal diseases and bacterial diseases. The fungal diseases include those caused by *Phytophthora capsici*, *Pythium spp.*, *Botrytis cinerea*, *Alternaria alternata* (causing early blight in potatoes), and *Curvularia zedoaria*, etc.; the bacterial diseases include those caused by *Ralstonia solanacearum* (causing bacterial wilt in tomatoes), *Erwinia carotenoides* (causing soft rot in cabbage and kale), *Xanthomonas oryzae* (causing rice streak), and *Pseudomonas syringae* (causing bacterial leaf blight in wheat), etc.
[0014] The fifth technical solution of this application provides the application of the Pentosacchariphyte ZNRZ-B053 or microbial inoculants in promoting plant growth.
[0015] Preferably, the plants include grain crops and vegetable crops. The *Pediococcus pentosaceus* ZNRZ-B053 or the microbial inoculant is applied to seeds, seedlings, flowers, fruits, etc., and can be applied by spraying, foliar spraying, soaking, or root irrigation. The promotion of plant growth includes promoting seed germination, promoting root growth, increasing crop yield, and improving crop quality.
[0016] The beneficial effects of this invention are as follows:
[0017] The *Pediococcus pentosaceus* ZNRZ-B053 isolated from kimchi brine in this invention can grow well in environments of 15-45℃ or pH 4-8, and can grow vigorously in environments of 25-40℃ or pH 5-7, and can adapt to complex soil environments.
[0018] The *Pediococcus pentosaceus* ZNRZ-B053 provided by this invention has good phosphorus and potassium solubilizing abilities. It can attach well, survive and reproduce on the leaves and rhizosphere of plants. It also shows good antibacterial activity against a variety of fungal and bacterial pathogens. When used in the growth and development of plants such as rice, cabbage, bok choy, and cauliflower, it can promote seed germination and root growth, thereby significantly increasing plant yield and improving plant quality. Attached Figure Description
[0019] Figure 1 Comparison of rice seed germination after treatment with different concentrations of bacterial solution;
[0020] Figure 2 Comparison of rooting status of cabbage seedlings before and after application of bacterial solution;
[0021] Figure 3 A diagram showing the growth of green vegetables before and after the use of bacterial solution;
[0022] Figure 4 This is a diagram showing the growth of cauliflower before and after the use of bacterial solution. Detailed Implementation
[0023] To make the objectives, technical solutions, and advantages of this invention clearer, the following detailed description of the invention is provided in conjunction with embodiments. Those skilled in the art will understand that the following embodiments are merely exemplary and not intended to limit the scope of the invention. Furthermore, in the following description, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are followed. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.
[0024] The culture medium and preparation method used in the embodiments of the present invention are as follows:
[0025] MRS liquid medium (pH range 6.2-6.4): 10 g peptone, 10 g beef extract, 5 g yeast extract, 40 g sucrose, 2 g K₂HPO₄, 2 g C₆H₂O 14 N2O7, 5 g CH3COONa, 0.25 g MgSO4, 0.25 g MnSO4, 1 mL Tween 80, add ultrapure water to 1000 mL, and autoclave at 115℃ for 30 min.
[0026] MRS solid medium: 10 g peptone, 10 g beef extract, 5 g yeast extract, 40 g sucrose, 2 g K₂HPO₄, 2 g C₆H₂O 14 N2O7, 5 g CH3COONa, 0.25 g MgSO4, 0.25 g MnSO4, 1 mL Tween 80, 15 g agar, add ultrapure water to 1000 mL, autoclave at 115℃ for 30 min;
[0027] PDA medium: Take 6 g potato extract powder, 20 g glucose, and 15 g agar powder, add ultrapure water to 1000 mL, and autoclave at 120℃ for 20 min.
[0028] LB solid medium: Take 5 g yeast powder, 10 g tryptone, 10 g NaCl, 15 g agar powder, add ultrapure water to 1000 mL; autoclave at 120℃ for 20 min.
[0029] Example 1: Isolation and identification of Pediococcus pentosaceus ZNRZ-B053
[0030] 1. Strains screening and purification
[0031] Take a sample of pickled vegetable brine, add sterile physiological saline, shake thoroughly, and then dilute to different concentration gradients (10). -1 -10 -6 100 μL of each sample was spread onto MRS solid medium and incubated at 32°C for 48 h. Single strains with colony morphology, color and appearance consistent with the physiological morphology of Pediococcus were selected and isolated by streaking multiple times on MRS solid medium to obtain 17 purified strains.
[0032] The 17 purified strains obtained above were inoculated into MRS liquid medium at a 1% inoculum and cultured overnight. The culture medium concentration was then adjusted to 10. 6 -10 7 After CFU / mL concentration, 0.1 mL was spread onto MRS solid medium containing 0.5% calcium phosphate and incubated at 32°C for 48 h. Phosphate-solubilizing transparent zones appeared around strains with phosphate-solubilizing ability; the larger the transparent zone, the better the phosphate-solubilizing ability of the strain. Therefore, based on the size of the phosphate-solubilizing transparent zone, nine strains with good phosphate-solubilizing ability were initially screened.
[0033] Nine strains initially screened were spread (0.1 mL) onto MRS solid medium containing 0.5% calcium phosphate and MRS solid medium containing 1% potassium feldspar powder (200-300 mesh), respectively. The spread media were then subjected to high-temperature-microwave mutagenesis: the medium was first incubated at 50 °C for 2 h, then microwaved at 300 W for 2 min, and finally incubated at 32 °C for 48 h. Strains with potassium-solubilizing ability also exhibited potassium-solubilizing transparent zones; the larger the transparent zone, the better the potassium-solubilizing ability of the strain. The size of the phosphorus-solubilizing and potassium-solubilizing transparent zones on each medium was observed. Finally, the strain with the best phosphorus-solubilizing and potassium-solubilizing abilities (phosphorus-solubilizing transparent zone diameter of 10.3 mm and potassium-solubilizing transparent zone diameter of 6.6 mm) was selected as the target strain for further research.
[0034] 2. Identification of strains
[0035] The target strains were identified using the following method: genomic DNA was extracted from the strains and amplified by PCR. The primer information is shown in Table 1, and the PCR reaction system and conditions are shown in Tables 2 and 3, respectively. The PCR products obtained were purified by agarose gel electrophoresis and then sequenced. The sequencing results were compared with NCBI-BLAST. The NCBI website is: http: / / www.ncbi.nlm.gov / .
[0036] Table 1 Primer Information
[0037] .
[0038] Table 2 PCR reaction system
[0039] .
[0040] Table 3 PCR reaction conditions
[0041] .
[0042] Upon identification, the nucleotide sequence of the 16S rDNA of the target strain screened in this invention is shown in SEQ ID NO: 1, named *Pediococcus pentosaceus* ZNRZ-B053, and deposited on November 3, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO. 36457, and classified as *Pediococcus pentosaceus*. Pediococcuspentosaceus , .
[0043] Example 2: Study on strain characteristics
[0044] 1. Determination of the temperature tolerance of the strain
[0045] After activation, *Pediococcus pentosaceus* ZNRZ-B053 was inoculated into MRS liquid medium at a 1% inoculum and cultured on a shaker at 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, 40℃, 45℃, 50℃, and 55℃ for 48 h at 200 rpm. The OD of the culture medium was measured after the culture was completed. 600 The values were used to evaluate the growth of the strain at different temperatures. The results are shown in Table 4. The *Pediococcus pentosaceus* strain of the present invention can grow well in an environment of 15-45℃, and can grow vigorously in an environment of 25-40℃.
[0046] Table 4. OD values of Pediococcus pentosaceus ZNRZ-B053 culture medium at different culture temperatures 600 value
[0047] .
[0048] 2. Determination of the strain's pH tolerance
[0049] After activation, *Pediococcus pentosaceus* ZNRZ-B053 was inoculated at a 1% inoculum into MRS liquid medium at pH values of 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, and 9. The pH of the medium was adjusted using lactic acid or sodium hydroxide solution (1 mol / L). The medium was incubated at 32°C with shaking (200 rpm) for 48 h. After incubation, the OD of the culture medium was measured. 600 The values were used to evaluate the growth of the strain under different pH conditions. The results are shown in Table 5. The *Pediococcus pentosaceus* strain of the present invention can grow well in environments with pH 4-8, and can grow vigorously in environments with pH 5-7.
[0050] Table 5. OD values of Pediococcus pentosaceus ZNRZ-B053 culture medium at different culture temperatures 600 value
[0051] .
[0052] In the table above, " / " represents OD. 600 When the value is close to 0, the strain hardly grows.
[0053] 3. Determination of the colonization ability of the strain
[0054] Colonization ability refers to the ability of a bacterial strain to successfully attach, survive, and reproduce in specific parts of a plant (such as the leaf surface or rhizosphere), forming a stable population. The stronger the colonization ability of a bacterial strain, the more beneficial it is to promoting plant growth.
[0055] (1) Leaf colonization ability
[0056] After activation, *Pediococcus pentosaceus* ZNRZ-B053 was inoculated into MRS liquid medium and cultured at 32°C on a shaker (200 rpm) until the concentration of the culture medium reached 10. 8 CFU / mL was used to obtain a bacterial suspension.
[0057] Select healthy tomato seedlings that have reached the 4-leaf stage. Rinse the leaves with sterile water to remove surface impurities. Using a spray method, evenly spray the bacterial suspension onto both sides of the leaves until the leaves are completely moistened but not dripping wet. Then, incubate at 25℃. Samples are taken at 1, 3, 7, 14, and 21 days to determine the colonization rate of the bacterial strain. The determination method is as follows:
[0058] Randomly weigh 1 g of leaf, add 10 mL of sterile physiological saline, and shake for 30 min to elute colonizing bacteria, obtaining the eluent. Rehydrate the eluent with sterile physiological saline for 10 minutes. 1 -10 5 Serial dilutions were performed, with 100 μL of each dilution spread onto MRS solid medium containing 200 μg / mL rifampicin. After incubation at 32℃ for 48 h, the number of colonies on the medium was counted (selecting a medium with a colony count of 30-300). The leaf colony density was calculated using the following formula:
[0059] Leaf colony count (CFU / g) = number of colonies on plate × dilution factor × volume of elution buffer.
[0060] The colonization results of Pediococcus pentosaceus ZNRZ-B053 on tomato leaves are shown in Table 6. As the number of culture days increased, the colonization number of the strain on the leaves decreased, but after 21 days of culture, the colonization number remained at 10. 4 CFU / g or higher indicates that the *Pediococcus pentosaceus* strain of the present invention has the ability to stably colonize plant leaves.
[0061] Table 6. Results of bacterial colonization on tomato leaves at different culture days.
[0062] .
[0063] (2) Rhizosphere colonization capacity
[0064] Healthy tomato seedlings at the 4-leaf stage were selected, and the bacterial strain was inoculated using the root irrigation method. Specifically, the soil was loosened to expose part of the root system, and the above-mentioned bacterial suspension was slowly poured around the roots at a rate of 10 mL / plant. The seedlings were then cultured at 25℃. 1 g of root samples were collected at 1, 3, 7, 14, and 21 days to determine the colonization rate of the bacterial strain. The determination method was the same as that for determining the colonization rate on leaves.
[0065] The colonization results of Pediococcus pentosaceus ZNRZ-B053 in the rhizosphere of tomato are shown in Table 7. With increasing culture days, the colonization number of the strain in the rhizosphere decreased, but after 21 days of culture, the colonization number remained at 10. 5 CFU / g or higher indicates that the *Pediococcus pentosaceus* strain of the present invention also has the ability to stably colonize in the plant rhizosphere.
[0066] Table 7. Results of bacterial colonization in the rhizosphere of tomato under different culture days.
[0067] .
[0068] Example 3: Application of Pediococcus pentosaceus ZNRZ-B053 in the control of plant diseases
[0069] (1) Antimicrobial activity of Pediococcus pentosaceus ZNRZ-B053 against fungal pathogens
[0070] The inhibitory activity of Pediococcus pentosaceus ZNRZ-B053 against Phytophthora capsici, Pythium spp., Botrytis cinerea, Alternaria alternata, and Curvularia zeylans was determined using the following method:
[0071] After activation, *Pediococcus pentosaceus* ZNRZ-B053 was inoculated into MRS liquid medium at a 1% inoculum size and cultured on a shaker at 32°C (200 rpm) for 48 h. The supernatant was collected by centrifugation (5000 rpm, 10 min) and filtered through a 0.22 μm filter to obtain cell-free fermentation broth. PDA medium was poured into sterile petri dishes to prepare agar plates. A 5 mm diameter mycelial disc was taken from the edge of a pre-cultured fungal pathogen colony and inoculated into the center of the solidified plate (mycelial side down). In the experimental group, four sterilized Oxford cups were placed at equal intervals around the central mycelial disc (2.5-3 cm away), and 200 μL of cell-free fermentation broth was added to each. The control group consisted of plates inoculated only with pathogen mycelial discs. The plates of the experimental group and the control group were incubated at 28°C. When the colonies of the control group were about to fill the petri dish, the colony diameters of the control group (D1) and the experimental group (D2) were measured. Then, the mycelial growth inhibition rate of Pediococcus pentosacchari ZNRZ-B053 against various fungal pathogens was calculated according to the following formula. The results are shown in Table 8. It can be seen that Pediococcus pentosacchari of the present invention has a significant inhibitory effect on a variety of fungal pathogens.
[0072] Mycelial growth inhibition rate (%) = (D1-D2) / D1×100%.
[0073] Table 8. Results of antifungal activity assay of Pediococcus pentosaceus ZNRZ-B053 against several fungal pathogens.
[0074] .
[0075] (2) Antibacterial activity of Pediococcus pentosaceus ZNRZ-B053 against bacterial pathogens
[0076] The inhibitory activity of Pediococcus pentosaceus ZNRZ-B053 against Ralstonia solanacearum, Erwinia carotenoides, Xanthomonas oryzae (the pathogen that causes rice streak), and Pseudomonas syringae was determined using the following method:
[0077] Take 100 μL of bacterial pathogen spore suspension (10 6 -10 7 The culture medium (CFU / mL) was mixed with 20 mL of LB medium to prepare a plate. Four sterilized Oxford cups were placed at equal intervals on the solidified plate, and 100 μL of cell-free fermentation broth was added. The plates were then incubated at 30 °C for 24 h. The diameter of the inhibition zone around the Oxford cup in each plate was measured. The larger the diameter, the stronger the antibacterial activity. The results are shown in Table 9. It can be seen that the *Pediococcus pentosaceus* of this invention has a significant inhibitory effect on a variety of bacterial pathogens.
[0078] Table 9. Results of antibacterial activity assay of Pediococcus pentosaceus ZNRZ-B053 against several bacterial pathogens.
[0079] .
[0080] Example 4: Application of Pediococcus pentosaceus ZNRZ-B053 in promoting rice seed germination
[0081] Select plump, disease-free rice seeds, disinfect their surface with 75% alcohol, then completely immerse them in the treatment solution described in Table 10. After soaking for 60 minutes, culture them in alternating light and dark conditions at 25℃ for 7 days. Seed germination is as follows: Figure 1 As shown in the figure, the rice seed buds treated with Pediococcus pentosaceus ZNRZ-B053 bacterial solution were significantly longer than those of untreated seeds, especially those treated with medium and high concentrations of the bacterial solution. This indicates that Pediococcus pentosaceus of the present invention can effectively promote plant seed germination, demonstrating excellent germination-promoting effects.
[0082] Table 10 Composition of Rice Seed Soaking Solution
[0083] .
[0084] Example 5: Application of Pediococcus pentosaceus ZNRZ-B053 in promoting rooting of cabbage
[0085] Select uniformly grown cabbage seedlings (2-3 leaf stage), remove soil from the roots, trim away old fibrous roots (retain the main root, about 2 cm in length), rinse thoroughly with 75% alcohol, and then immerse the roots in a 10% alcohol solution. 6The seedlings were soaked in a solution of *Pediococcus pentosaceus* ZNRZ-B053 (CFU / mL) for 60 minutes, then removed and blotted dry with sterile filter paper. After 21 days of alternating light and dark culture at 25°C, the rooting status of the cabbage seedlings was as follows: Figure 2 As shown in the figure, "After use" indicates soaking treatment with bacterial solution, and "Before use" indicates no soaking treatment with bacterial solution. From Figure 2 It can be seen that the root length of the cabbage seedlings after use is nearly twice that of the cabbage seedlings before use, which indicates that the Pediococcus pentosaceus of the present invention has a significant root-promoting effect on plants.
[0086] Example 6: The role of Pediococcus pentosaceus ZNRZ-B053 in promoting the growth of leafy greens
[0087] Select plump, disease-free vegetable seeds, disinfect their surface with 75% alcohol, and then completely immerse them in a 10% alcohol solution. 6 After soaking in a CFU / mL solution of *Pediococcus pentosaceus* ZNRZ-B053 for 60 minutes to promote germination, the seeds were sown in substrate soil when the radicle reached 0.5 cm in length and cultured at 25℃ under alternating light and dark conditions. When the seedlings reached the 2-3 leaf stage, they were drenched once with the same concentration of bacterial solution at a rate of 10 mL per plant (experimental group). Seeds of bok choy that were not soaked or drenched served as the control group. The growth of bok choy in the experimental and control groups after 30 days of culture is shown below. Figure 3 As shown in the diagram, "After use" indicates soaking and root drenching with the bacterial solution, while "Before use" indicates no soaking or root drenching with the bacterial solution. Figure 3 It can be seen that the growth of the bok choy treated with the bacterial solution was significantly better than that of the untreated bok choy, with a significant increase in plant volume. Further measurements were taken on the average root length and yield of 100 bok choy plants. The results are shown in Table 11. The bok choy treated with the Pediococcus pentosaceus bacterial solution of this invention showed an average increase in root length of 37.96% and an increase in yield of 42.03%.
[0088] Table 11 Results of root length and yield measurement of Chinese cabbage under different treatments
[0089] .
[0090] Example 7: The role of Pediococcus pentosaceus ZNRZ-B053 in promoting cauliflower growth
[0091] Select plump, uniform cauliflower seeds free from pests and diseases. After surface disinfection with 75% alcohol, completely immerse them in a solution of 10% alcohol. 6After soaking in a CFU / mL solution of *Pediococcus pentosaceus* ZNRZ-B053 for 60 min, the cauliflower was sown in substrate soil and cultured under alternating light and dark conditions at 25℃. When the seedlings reached 3 leaves and 1 bud, seedlings of uniform growth were selected for transplanting. At transplanting, the roots were drenched once with the same concentration of bacterial solution to strengthen colonization (5 mL / plant). Fifteen days after transplanting, during the rosette stage, the roots were drenched again with the same concentration of bacterial solution (15 mL / plant) (experimental group). Cauliflower seeds that were not soaked or drenched served as the control group. Harvesting was carried out when the flower heads were fully enlarged (70 days post-sowing). The growth of cauliflower in the experimental and control groups is shown below. Figure 4 As shown in the diagram, "After use" indicates soaking and root drenching with the bacterial solution, while "Before use" indicates no soaking or root drenching with the bacterial solution. Figure 4 It can be seen that the cauliflower heads increased significantly after treatment with the bacterial solution. Further measurements were taken of the single-head weight and whiteness of 100 cauliflower heads, and the average value was used. Whiteness was measured using a colorimeter. The results of each indicator are shown in Table 12. Cauliflower treated with the *Pediococcus pentosaceus* bacterial solution of this invention not only showed a significant increase in single-head weight but also improved whiteness, resulting in a significant improvement in overall quality.
[0092] Table 12 Results of single head weight and whiteness determination of cauliflower under different treatments
[0093] .
[0094] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.
Claims
1. A strain of Pediococcus pentosaceus ( Pediococcus pentosaceus ZNRZ-B053, characterized in that, This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 3, 2025, with accession number CGMCC NO.36457. The strain has the ability to solubilize phosphorus and potassium and can stably colonize on the leaves and rhizosphere of plants.
2. A microbial inoculant, characterized in that, Contains Pediococcus pentosaceus as described in claim 1 ( Pediococcus pentosaceus ZNRZ-B053.
3. The microbial agent as described in claim 2, characterized in that, The microbial agent contains Pediococcus pentosaceus ( Pediococcus pentosaceus ZNRZ-B053 has a viable count ≥10-10 5 CFU / mL or ≥10 5 CFU / g.
4. The microbial agent as described in claim 2, characterized in that, The microbial agent is in liquid, semi-liquid, or solid form.
5. The Pediococcus pentosaceus as described in claim 1 ( Pediococcus pentosaceus The application of ZNRZ-B053, or any one of claims 2-4, in soil conditioning, is characterized in that... The soil conditioning process involves converting insoluble phosphorus and potassium in the soil into readily available phosphorus and potassium that can be absorbed by plants.
6. The Pediococcus pentosaceus as described in claim 1 ( Pediococcus pentosaceus The application of ZNRZ-B053, or any one of claims 2-4, in the control of plant diseases, is characterized in that... The plant diseases mentioned are fungal diseases and bacterial diseases. The pathogens of the fungal diseases are Phytophthora capsici, Pythium spp., Botrytis cinerea, Alternaria alternata, and Curvularia zeylanica. The pathogens of the bacterial diseases are Ralstonia solanacearum, Erwinia carotenoides, Xanthomonas oryzae, and Pseudomonas syringae.
7. The Pediococcus pentosaceus as described in claim 1 ( Pediococcus pentosaceus The application of ZNRZ-B053, or any one of claims 2-4, in promoting plant growth, is characterized in that... The promotion of plant growth refers to promoting rice seed germination, promoting root growth in vegetable crops, and increasing vegetable crop yield.