Method and system for detecting medicament distribution on surface of modified colloid bubble

Abstract

The invention discloses a method and a system for detecting medicament distribution on the surface of modified colloid bubbles, and belongs to the field of colloid chemical analysis. According to the technical scheme, the method comprises the following steps: step 1, adding a preset mass of medicament into a surfactant solution with a certain concentration and a certain volume to prepare modified colloid bubbles containing a foam layer and tail liquid; 2, transferring a foam layer in the modified colloid bubbles into a first container, and naturally defoaming to obtain a to-be-detected liquid I; transferring the tail liquid in the modified colloid bubbles into a second container to obtain a to-be-detected liquid II; step 3, measuring the content of the medicament in the to-be-measured liquid II; step 4, based on the law of conservation of amount of substance, respectively calculating to obtain the distribution of the medicament in the modified colloidal bubble foam layer and the tail liquid so as to analyze the adsorption law of the medicament on the surface of the modified colloidal bubble and reveal the medicament distribution condition on the surface of the colloidal bubble; the method is applied to research and regulation of colloid bubble surface properties.

Description

Technical Field

[0001] This invention relates to the field of colloidal chemical analysis technology, specifically to a method and system for detecting the distribution of reagents on the surface of modified colloidal bubbles. Background Technology

[0002] Colloidal bubbles, due to their large interfacial area, high density, controllable size, and easily modifiable surface, are widely used in oilfield chemistry, biomedicine, materials synthesis, and separation and purification. Current research largely focuses on modifying the surface properties of colloidal bubbles and their impact on application performance, but the distribution patterns of reagents (such as electrolytes) within the colloidal bubbles themselves are insufficiently studied. The distribution of reagents on the bubble surface and in the liquid phase directly affects the bubble's stability, interfacial activity, and other core properties. Therefore, it is urgent to establish a precise method for detecting the distribution of reagents on the surface of modified colloidal bubbles, providing theoretical support for revealing the regulatory mechanisms of bubble surface properties. Summary of the Invention

[0003] To address the technical problem of clearly identifying the distribution of reagents on the surface of colloidal bubbles, this invention proposes a simple and reliable method and system for detecting the distribution of reagents on the surface of modified colloidal bubbles.

[0004] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: a method for detecting the distribution of reagents on the surface of modified colloidal bubbles, comprising the following steps: Step 1: Preparation of modified colloidal bubbles: Add a predetermined amount of reagent to a surfactant solution of a certain concentration and volume to prepare modified colloidal bubbles containing a foam layer and tail liquid. Step 2, separation of foam layer and tail liquid: Transfer the foam layer in the modified colloidal bubbles to the first container and allow it to defoam naturally to obtain test liquid one; transfer the tail liquid in the modified colloidal bubbles to the second container to obtain test liquid two; Step 3: Detection of reagent content in the test solution: Determine the reagent content in test solution 2; Step 4: Calculation of reagent distribution: Based on the law of conservation of mass, the distribution of the reagent in the modified colloidal bubble foam layer and the tail liquid is calculated to analyze the adsorption law of the reagent on the surface of the modified colloidal bubble.

[0005] Furthermore, the surfactant is any one of polyoxyethylene type, fatty acid ester of polyol, alkylolamide, amine oxide and sulfoxide.

[0006] Furthermore, the concentration range of the surfactant is 0.1~10 mmol·L⁻¹. -1 .

[0007] Furthermore, the preparation of the modified colloidal bubbles containing the foam layer and tailings in step one is achieved by injecting the bubble-generating liquid obtained by mixing the surfactant solution and the reagent into a bubble-generating tank at a rate of 5000~8000 r·min. -1 Stir at high speed for 0.1~10 minutes.

[0008] Furthermore, the agent is an electrolyte, and the amount of the agent is 0.1~100 mM.

[0009] Furthermore, in step two, the methods for separating the foam layer from the tail liquid include, but are not limited to, using a peristaltic pump for extraction or a foam scraping method.

[0010] Furthermore, in step three, the detection method for the content of the reagent in the test solution two includes, but is not limited to, one or more of the following: chemical titration, ultraviolet spectrophotometry, and chemical calculation.

[0011] Furthermore, in step four, the distribution of the agent in the foam layer and the tail liquid is calculated using the following formulas: Total amount of drug substance: n 药剂 =m / M, where m is the mass of the drug and M is the relative molecular mass of the drug; Gas content: H hold =H T -H0, where H T H0 is the final height of the foam layer, and H0 is the initial solution height. Tailings volume: V W =V initial *(1-H hold In the formula, V initial H is the initial volume of the bubble-generating liquid. hold Gas holdup; Amount of pharmaceutical substance in the tail liquid: n W =C W *V W In the formula, C W V represents the concentration of the reagent in the tailings liquid. W This represents the volume of the tail liquid. Amount of pharmaceutical substance in the foam layer: n CGA =n 药剂 -n W In the formula, n 药剂 n represents the total amount of substance of the pharmaceutical preparation. W The amount of pharmaceutical substance in the tail liquid; The proportion of the agent in the modified colloidal bubble foam layer: Γ CGA =n CGA / n 药剂 ; The proportion of the reagent in the modified colloidal bubble tail liquid: Γ W =nW / n 药剂 .

[0012] Furthermore, electrolytes include, but are not limited to, inorganic salt ions or their hydroxides.

[0013] A system for detecting the surface distribution of the modified colloidal bubble as described above includes: a bubble generating pool for preparing modified colloidal bubbles containing a foam layer and a tail liquid, and a third container for determining the magnesium ion concentration in the colloidal bubble tail liquid. The side wall of the bubble generating tank is provided with a first through hole and a second through hole. The first through hole is connected to the first container through a first peristaltic pump, and the second through hole is connected to the second container through a second peristaltic pump.

[0014] The beneficial effects of this invention compared to the prior art are as follows: This invention provides a method and system for detecting the distribution of reagents on the surface of modified colloidal bubbles. By separating the bubble foam layer from the tail liquid, detecting the reagent content, and calculating the distribution ratio in combination with the conservation law, the adsorption law of the reagent on the surface of modified colloidal bubbles is revealed. Attached Figure Description

[0015] The present invention will be further described below with reference to the accompanying drawings: Figure 1 This is a schematic diagram of the system structure of the present invention; Figure 2 This is a graph showing the relationship between the magnesium ion concentration and the proportion of magnesium ions on the surface of colloidal bubbles in this invention. In the diagram: 1 is the first peristaltic pump, 2 is the second peristaltic pump, 3 is the bubble generating tank, 4 is the first container, 5 is the second container, 6 is the third container, 7 is the iron stand, 8 is the butterfly clamp, and 9 is the burette. Detailed Implementation

[0016] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate relative orientations or positional relationships and are used only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0017] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art will understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0018] like Figures 1 to 2 As shown, the present invention provides a method for detecting the distribution of reagents on the surface of modified colloidal bubbles, comprising the following steps: Step 1: Preparation of modified colloidal bubbles: A predetermined amount of reagent is added to a surfactant solution of a certain concentration and volume to prepare a bubble-generating solution. This bubble-generating solution is then injected into bubble-generating tank 3 at a rate of 5000~8000 r·min. -1 The mixture is stirred at high speed for 0.1–10 min to form a modified colloidal bubble system containing a foam layer and a tailings liquid. The reagent used is an electrolyte, including but not limited to inorganic salt ions or their hydroxides, with a concentration of 0.1–100 mM. The surfactant concentration ranges from 0.1–10 mmol·L⁻¹. -1 .

[0019] Step 2: Separation of foam layer and tail liquid: The foam layer and tail liquid are separated by peristaltic pump extraction or foam scraping method. In one embodiment, the foam layer is transferred to a container by peristaltic pump and allowed to defoam naturally, which is then used as test liquid one (the reagent in the foam layer); the remaining tail liquid (liquid phase) is transferred to another container as test liquid two (the reagent in the liquid phase).

[0020] Step 3: Detection of reagent content in the test solution: For test solution 2, chemical methods such as colorimetric reaction and complexation reaction (e.g., EDTA complexometric titration) are used, combined with ultraviolet spectrophotometer or chemical calculation, to determine the concentration of the reagent.

[0021] Step 4: Calculation of reagent distribution: Based on the law of conservation of mass, the distribution of the reagent in the foam layer and the tail liquid is calculated using the following formulas: Total amount of drug substance: n 药剂 =m / M, where m is the mass of the drug and M is the relative molecular mass of the drug; Amount of pharmaceutical substance in the tail liquid: n W =C W *V W In the formula, C W V represents the concentration of the reagent in the tailings liquid. W This represents the volume of the tail liquid. Tailings volume: V W=V initial *(1-H hold In the formula, V initial H is the initial volume of the bubble-generating liquid. hold Gas holdup; Gas content: H hold =H T -H0, where H T H0 is the final height of the foam layer, and H0 is the initial solution height. Amount of pharmaceutical substance in the foam layer: n CGA =n 药剂 -n W In the formula, n 药剂 n represents the total amount of substance of the pharmaceutical preparation. W The amount of pharmaceutical substance in the tail liquid; The proportion of the agent in the modified colloidal bubble foam layer: Γ CGA =n CGA / n 药剂 ; The proportion of the reagent in the modified colloidal bubble tail liquid: Γ W =n W / n 药剂 .

[0022] The present invention will be further illustrated by the following specific embodiments, but the scope of protection of the present invention is not limited to the following embodiments. Example 1:

[0023] This embodiment detects the distribution of magnesium ions on the surface of modified colloidal bubbles, including the following steps: Step 1: Preparation of modified colloidal bubbles: Add 2g of magnesium chloride to a 135mL solution of dodecyl alcohol polyoxyethylene ether (surfactant solution) with a concentration of 0.35mmol / L, and stir to dissolve to obtain a bubble generating liquid; then transfer the obtained bubble generating liquid to bubble generating tank 3 and stir at 8000 r / min for 3min to form modified colloidal bubbles containing a foam layer and tail liquid.

[0024] Step 2: Separate the test solution Adjust the speed of peristaltic pump B to 12.3 L / min, and extract the foam layer into the first container 4. After the foam layer is defoamed in the first container 4, it is used as the test liquid one. Adjust the peristaltic pump C to extract the tail liquid into the second container 5, which is used as the test liquid two.

[0025] Step 3: Detect the magnesium ion concentration in test solution 2: Take 1 mL of the test solution and transfer it to a 25 mL volumetric flask, then add water to bring the volume to 25 mL. Transfer the diluted solution to a third container (6). Add 4 mL of NH4Cl buffer and 3 drops of Eriochrome Black T indicator to the third container (6). At this point, the solution in the third container (6) will turn purple. Then titrate with 10 mmol / L disodium EDTA solution until the solution changes from purple to bright blue. Record the volume of disodium EDTA solution consumed as ΔV.

[0026] The magnesium ion concentration in the tailings was calculated based on the volume ΔV of the consumed EDTA disodium solution: C W =C1*ΔV / V2, where C1 is the concentration of the EDTA disodium solution and V2 is the sampling volume of the test solution in mL.

[0027] Step 4: Calculation of reagent distribution: Based on the law of conservation of mass, the distribution of magnesium ions in the foam layer and the tail liquid is calculated using the following formulas: Total amount of magnesium ions: n 药剂 =m / M=20mmol; Gas content: H hold =H T -H0=60%; Tailings volume: V W =V initial *(1-H hold ) = 54 mL; Amount of magnesium ions in the tailings: n W =C W *V W =6.318±0.189mmol; The amount of magnesium ions in the foam layer: n CGA =n 药剂 -n W =13.682±0.189mmol; The proportion of magnesium ions in the modified colloidal bubble foam layer: Γ CGA =n CGA / n 药剂 =67.46%±0.95%; The proportion of magnesium ions in the modified colloidal bubble tail liquid: Γ W =n W / n 药剂 =32.53%±0.95%.

[0028] The results of this embodiment show that most magnesium ions are adsorbed in the modified colloidal bubble foam layer. Example 2:

[0029] This embodiment modifies the dosage of the reagent and detects the distribution of magnesium ions, including the following steps: Step 1: Preparation of modified colloidal bubbles: Add 4g of magnesium chloride to a 135mL solution of dodecyl alcohol polyoxyethylene ether with a concentration of 0.35mmol / L, and stir to dissolve to obtain a bubble generating liquid. Then transfer the obtained bubble generating liquid to bubble generating tank 3 and stir at 8000r / min for 3min to form modified colloidal bubbles containing a foam layer and tail liquid.

[0030] The procedure for separating the test solution and detecting the magnesium ion concentration in test solution two is the same as step two and step three in Example 1.

[0031] Step 4: Calculation of reagent distribution: Based on the law of conservation of mass, the distribution of magnesium ions in the foam layer and the tail liquid is calculated using the following formulas: Total amount of magnesium ions: n 药剂 =m / M=40mmol; Gas content: H hold =H T -H0=60%; Tailings volume: V W =V initial *(1-H hold ) = 54 mL; Amount of magnesium ions in the tailings: n W =C W *V W =12.366±0.378 mmol; The amount of magnesium ions in the foam layer: n CGA =n 药剂 -n W =27.366±0.378mmol; The proportion of magnesium ions in the modified colloidal bubble foam layer: Γ CGA =n CGA / n 药剂 =69.09%±0.95%; The proportion of magnesium ions in the modified colloidal bubble tail liquid: Γ W =n W / n 药剂 =30.91%±0.95%.

[0032] The results showed that with increasing magnesium ion concentration, the proportion of magnesium ions in the modified colloidal bubble foam layer increased. Figure 2 It can be seen that when the magnesium ion concentration exceeds 10 mM, as the magnesium ion concentration increases, the rate at which the proportion of magnesium ions in the modified colloidal bubble foam layer increases gradually decreases, the proportion of magnesium ions in the modified colloidal bubble foam layer tends to stabilize, and the proportion of magnesium ions on the surface of the colloidal bubble does not exceed 75%.

[0033] The method of this invention achieves accurate determination of the agent distribution on the surface of modified colloidal bubbles through stepwise separation, quantitative detection and conservation calculation. It is simple to operate and the results are reliable, providing key methodological support for studying the interaction between agents and bubble surfaces and optimizing bubble surface properties.

[0034] The system provided by the present invention for detecting the surface distribution of the modified colloidal bubble agent as described above includes: a bubble generating pool 3 for preparing modified colloidal bubbles containing a foam layer and a tail liquid, and a third container 6 for determining the magnesium ion concentration in the colloidal bubble tail liquid by titration; a first through hole is provided on the upper side wall of the bubble generating pool 3, the first through hole is connected to the first container through a first peristaltic pump 1, and a second through hole is provided on the lower side wall of the bubble generating pool 3, the second through hole is connected to the second container 5 through a second peristaltic pump 2.

[0035] The third container 6 is placed on an iron stand 7, and a butterfly clamp 8 is fixedly mounted on the iron stand 7. A burette 9 is arbitrarily and fixably connected to the butterfly clamp 8, and the butterfly clamp 8 controls the burette 9 to be suspended above the opening of the third container 6. In one embodiment, the third container 6 is an Erlenmeyer flask.

[0036] Regarding the specific structure of this invention, it should be noted that the connection relationships between the various component modules used in this invention are definite and achievable. Except as specifically described in the embodiments, their specific connection relationships can bring about corresponding technical effects and solve the technical problems proposed by this invention without relying on the execution of corresponding software programs. The models of the components, modules, and specific components appearing in this invention, the connection methods between them, and the conventional usage methods and expected technical effects brought about by the above technical features, unless specifically described, are all publicly disclosed content in patents, journal articles, technical manuals, technical dictionaries, and textbooks that can be obtained by those skilled in the art before the application date, or belong to conventional technology, common knowledge, and other existing technologies in this field. There is no need to elaborate, which makes the technical solution provided in this case clear, complete, and achievable, and can reproduce or obtain corresponding physical products based on this technical means.

[0037] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for detecting the distribution of reagents on the surface of modified colloidal bubbles, characterized in that: Includes the following steps: Step 1: Preparation of modified colloidal bubbles: Add a predetermined amount of reagent to a surfactant solution of a certain concentration and volume to prepare modified colloidal bubbles containing a foam layer and tail liquid. Step 2, separation of foam layer and tail liquid: transfer the foam layer in the modified colloidal bubbles to the first container (4), and after it naturally defoams, obtain test liquid one; transfer the tail liquid in the modified colloidal bubbles to the second container (5) to obtain test liquid two; Step 3: Detection of reagent content in the test solution: Determine the reagent content in test solution 2; Step 4: Calculation of reagent distribution: Based on the law of conservation of mass, the distribution of the reagent in the modified colloidal bubble foam layer and the tail liquid is calculated to analyze the adsorption law of the reagent on the surface of the modified colloidal bubble.

2. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: The surfactant is any one of polyoxyethylene type, fatty acid ester of polyol, alkylolamide, amine oxide and sulfoxide.

3. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: The concentration range of the surfactant is 0.1~10 mmol·L. -1 .

4. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: The preparation of modified colloidal bubbles containing a foam layer and tail liquid in step one is as follows: the bubble generating liquid obtained by mixing surfactant solution and reagent is injected into bubble generating tank (3) at a speed of 5000~8000 r·min -1 Stir at high speed for 0.1~10 minutes.

5. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: The reagent is an electrolyte, and the amount of the reagent is 0.1~100mM.

6. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: In step two, the methods for separating the foam layer from the tail liquid include, but are not limited to, using a peristaltic pump for extraction or a foam scraping method.

7. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: In step three, the detection method for the content of the reagent in the test solution two includes, but is not limited to, one or more of the following: chemical titration, ultraviolet spectrophotometry, and chemical calculation.

8. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 1, characterized in that: In step four, the distribution of the agent in the foam layer and the tail liquid is calculated using the following formulas: Total amount of drug substance: n 药剂 =m / M, where m is the mass of the drug and M is the relative molecular mass of the drug; Gas content: H hold =H T -H0, where H T H0 is the final height of the foam layer, and H0 is the initial solution height. Tailings volume: V W =V initial *(1-H hold In the formula, V initial H is the initial volume of the bubble-generating liquid. hold Gas holdup; Amount of pharmaceutical substance in the tail liquid: n W =C W *V W In the formula, C W V represents the concentration of the reagent in the tailings liquid. W This represents the volume of the tail liquid. Amount of pharmaceutical substance in the foam layer: n CGA =n 药剂 -n W In the formula, n 药剂 n represents the total amount of substance of the pharmaceutical preparation. W The amount of pharmaceutical substance in the tail liquid; The proportion of the agent in the modified colloidal bubble foam layer: Γ CGA =n CGA / n 药剂 ; The proportion of the reagent in the modified colloidal bubble tail liquid: Γ W =n W / n 药剂 .

9. The method for detecting the distribution of reagents on the surface of modified colloidal bubbles according to claim 5, characterized in that: Electrolytes include, but are not limited to, inorganic salt ions or their hydroxides.

10. A system for detecting the distribution of reagents on the surface of modified colloidal bubbles as described in any one of claims 1-9, characterized in that, include: A bubble generating tank (3) for preparing modified colloidal bubbles containing a foam layer and tail liquid, and a third container (6) for determining the magnesium ion concentration in the colloidal bubble tail liquid. The side wall of the bubble generating tank (3) is provided with a first through hole and a second through hole. The first through hole is connected to the first container (4) through a first peristaltic pump, and the second through hole is connected to the second container (5) through a second peristaltic pump.

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