Mangosteen extract with private care function as well as preparation method and application of mangosteen extract

Abstract

The invention discloses a mangosteen extract with a private care function and a preparation method and application thereof, and belongs to the technical field of cosmetics, the mangosteen extract can be widely applied to the preparation process of female private care products, has the effects of cleaning, inhibiting bacteria and adjusting vaginal flora imbalance, and can be used for treating private parts. Further, inflammatory reaction is reduced, and the self-cleaning capacity of the vagina is improved.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, specifically to a mangosteen extract with intimate care functions, its preparation method, and its application. Background Technology

[0002] The female vagina is a physiological cavity connecting to the outside world, home to a rich variety and vast number of microorganisms. The host, normal flora, and natural environment are interdependent and mutually restrictive, forming a dynamically balanced micro-ecosystem. Under normal circumstances, vaginal microorganisms are dominated by Gram-positive lactobacilli. These existing microbiota, vaginal anatomy, local immunity, and the body's endocrine system collectively constitute the vaginal microenvironment. When the vaginal microenvironment is disrupted, a series of gynecological diseases can occur. Most of these diseases are caused by bacterial or fungal infections, leading to increased vaginal discharge, itching, pain, foul odor, and in severe cases, a series of complications.

[0003] Bacterial vaginosis is one of the most common lower genital tract infections in women, posing a significant threat to women's health. Increasing research indicates that the pathogens causing bacterial vaginosis include several anaerobic bacteria such as Gardnerella vaginalis, Prevotella vaginalis, and Atobacillus. However, Gardnerella vaginalis exhibits higher virulence potential, stronger adhesion and cytotoxicity, and is more prone to biofilm formation than other pathogens, playing a more crucial role in the pathogenesis of bacterial vaginosis.

[0004] Current treatment options for Gardnerella vaginalis primarily utilize anti-anaerobic drugs such as nitroimidazoles (metronidazole and tinidazole) and chemical drugs like clindamycin. These options suffer from high recurrence rates and can damage beneficial bacteria. Additionally, some all-natural, single-ingredient products offer limited efficacy and are prone to inducing drug resistance. While some compound products can address resistance issues, these are complex, involve numerous uncertainties, and are difficult to manufacture stably. Therefore, developing a feminine hygiene product that is effective, gentle, and stable is a crucial challenge in this field. Summary of the Invention

[0005] This invention provides a mangosteen extract with intimate care function, its preparation method and application. The mangosteen extract can be widely used in the preparation of female vaginal care products, and has the effects of cleaning and inhibiting bacteria, regulating vaginal flora imbalance, thereby reducing the occurrence of inflammatory reactions and improving the vagina's self-cleaning ability.

[0006] The present invention solves its technical problem by adopting the following technical solution: A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Mangosteen peel powder, cysteine, and citric acid were added to a solvent and subjected to reduced pressure ultrasonic extraction. The mixture was then filtered to obtain a filtrate, which was concentrated until no ethanol was present. Cysteine ​​served as a reducing agent to block the polymerization / degradation of α-dextrin. Citric acid chelated the metal ion Fe. 3+ / Cu 2+ (To avoid metal-catalyzed degradation of α-twistingin). (2) Adjust the pH of the filtrate to 4.2-4.8 with sodium citrate, add a compound enzyme for enzymatic hydrolysis, inactivate the enzyme, filter, concentrate, and obtain a concentrated solution. Add ethanol solution to a concentration of 30-40 wt%. Apply the solution to a macroporous adsorption resin at a flow rate of 1-2 BV / h. First, elute with 2-4 BV of water, then with 2-4 BV of 20-30 wt% ethanol solution, then with 3-4 BV of 50-60 wt% ethanol solution, and finally with 2-4 BV of 80-90 wt% ethanol solution. Collect the eluent, concentrate, and obtain a concentrated eluent. This step involves enzymatic hydrolysis... Specific degradation was performed to degrade the RG-I type structure and side chain of mangosteen pectin. Then, α-dextrin was dissolved and subjected to macroporous adsorption resin to remove water-soluble inorganic salts (citrate solution), sugar impurities, proteins and some pigments. Then, 20%-30% ethanol was used to remove medium polar flavonoids (such as kaempferol glycosides), phenolic acids (such as ellagic acid, gallic acid, etc.), and low molecular weight pigments (such as carotenoids and their derivatives). 50%-60% ethanol was used to remove some impurities with similar polarity to α-dextrin. 80%-90% ethanol was used to elute and obtain the α-dextrin component. (3) Add citrate buffer and chitosan to the concentrated eluent, stir evenly to obtain a complexing solution. Adjust the conductivity of the complexing solution to 78-82 μs / cm with water at 20-30℃, and crystallize by gradient cooling. Filter, wash, and dry to obtain mangosteen extract. Porous crystals of mangosteen α-dextrin can be obtained through complexation. Then, adjusting the conductivity and ordering the hydrogen bond network of water molecules will promote the formation of a stable "hydration bridge" structure of dextrin-chitosan, with the lowest lattice energy, inducing crystal nucleus generation. Gradient cooling program: 25℃→10℃ (rate 2℃ / h), hold for 2-6 hours at the endpoint, wash the crystals with 5% ethanol aqueous solution, and finally dry to obtain mangosteen α-dextrin crystals. In a preferred embodiment of the present invention, the mass ratio of mangosteen peel powder, cysteine, and citric acid is 1:(0.0005~0.002):(0.001~0.002).

[0007] In a preferred embodiment of the present invention, the solid-liquid ratio of the mangosteen peel powder and the solvent is 1g:(6~10)mL, and the solvent is 10%~20% ethanol.

[0008] In a preferred embodiment of the present invention, the temperature of the depressurized ultrasonic extraction is 40~50℃, the ultrasonic power is 400W, and the vacuum degree is -0.08 MPa.

[0009] In a preferred embodiment of the present invention, the time for depressurized ultrasonic extraction is 20-30 min.

[0010] In a preferred embodiment of the present invention, the mass ratio of the filtrate to the compound enzyme is 1:(0.002~0.004).

[0011] As a preferred embodiment of the present invention, the composite enzyme comprises cellulase, pectinase, arabinogalactanase, and xylanase in a mass ratio of (4~6):(4~6):(1~3):(0.5~2). Cellulase cleaves cell wall residues to release the embedded target substance. Pectinase can cleave the α-1,4 glycosidic bonds of mangosteen pectin and remove some colloidal impurities, but it is ineffective against rhamnogalacturonic acid polysaccharide (RG-I). Since the RG-I type structure accounts for 35% of mangosteen pectin, arabinogalactanase and xylanase are added to specifically degrade the side chains of RG-I to avoid some pectin adhering to the target component.

[0012] In a preferred embodiment of the present invention, the enzymatic hydrolysis temperature is 50~58℃ and the time is 1~4h; The enzyme inactivation temperature is 80~90℃, and the time is 6~12min.

[0013] In a preferred embodiment of the present invention, the volume ratio of the concentrated eluent to the citrate buffer is 1:(2~5). The mass ratio of the concentrated eluent to chitosan is 1:(0.0005~0.002). The molar concentration of the citrate buffer solution is 0.02~0.1 mol / L.

[0014] As a preferred embodiment of the present invention, the macroporous adsorption resin includes HZ818 macroporous adsorption resin (non-polar), HZ816 macroporous adsorption resin (non-polar), and HZ841 macroporous adsorption resin (weakly polar) in a mass ratio of (3~5):(3~5):(1~3).

[0015] The present invention also provides a mangosteen extract with intimate care function, which is prepared by the preparation method described above.

[0016] The present invention also provides the application of mangosteen extract with intimate care function in the preparation of cosmetics.

[0017] The beneficial effects of the present invention are: (1) The mangosteen extract described in the present invention is rich in α-dextrin. α-dextrin acts on the non-specific target of microbial membrane proteins, interferes with their energy metabolism, and reduces biofilm formation. Compared with the indiscriminate bactericidal effect of traditional antibiotics, α-dextrin can avoid drug resistance and has less damage to beneficial bacteria such as lactobacillus, which is conducive to maintaining the balance of vaginal microecology.

[0018] (2) This invention uses subcritical water to improve the extraction rate, and uses a compound enzyme to enzymatically hydrolyze mangosteen pectin to reduce the amount of loss caused by the adhesion and encapsulation of α-dextrin by pectin. It also uses a mixture of non-polar and weakly polar resins to enhance the adsorption and elution effect, and complexation crystallization to further improve the purity. This method can efficiently extract effective active ingredients and also easily produce products with good stability.

[0019] (3) The mangosteen extract described in this invention can be widely used in the preparation of female vaginal care products. It has the effects of cleaning and inhibiting bacteria, regulating vaginal flora imbalance, thereby reducing the occurrence of inflammatory reactions and improving the vagina's self-cleaning ability. Attached Figure Description

[0020] Figure 1 This is the HPLC chromatogram of Example 1.

[0021] Figure 2 This is the HPLC chromatogram of Comparative Example 1.

[0022] Figure 3 This is the HPLC chromatogram of Comparative Example 2.

[0023] Figure 4 This is the HPLC chromatogram of Comparative Example 3.

[0024] Figure 5 This is the HPLC chromatogram of Comparative Example 4.

[0025] Figure 6 This is the HPLC chromatogram of Comparative Example 5. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0028] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0029] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0030] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0031] Example 1 A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Dry and pulverize the mangosteen peel to 100 mesh to obtain mangosteen peel powder; Mangosteen peel powder, cysteine, and citric acid were added to a solvent and subjected to reduced pressure ultrasonic extraction. The mixture was then filtered to obtain a filtrate, which was concentrated until no ethanol was present. The mass ratio of mangosteen peel powder, cysteine, and citric acid was 1:0.0008:0.0015. The solid-liquid ratio of mangosteen peel powder to solvent was 1 g:8 mL, and the solvent was 15% ethanol.

[0032] (2) Adjust the pH of the filtrate to 4.5 with sodium citrate, add the compound enzyme and perform enzymatic hydrolysis at 55℃ for 2 hours, inactivate the enzyme at 85℃ for 8 minutes, filter, and concentrate to 10% of the original volume to obtain a concentrated solution; the mass ratio of the filtrate to the compound enzyme is 1:0.003. The compound enzyme includes cellulase, pectinase, arabinogalactanase and xylanase in a mass ratio of 5:5:2:1.

[0033] Add ethanol solution to the concentrate until the ethanol concentration is 35%, and feed it onto the macroporous adsorption resin at a rate of 1.5 BV / h. First, elute with 3 BV of water, then with 3 BV of 25 wt% ethanol solution, then with 3 BV of 60 wt% ethanol solution, and finally with 3 BV of 85 wt% ethanol solution. Collect the eluent, concentrate it, and obtain the concentrated eluent. The macroporous adsorption resins include HZ818 macroporous adsorption resin (non-polar), HZ816 macroporous adsorption resin (non-polar), and HZ841 macroporous adsorption resin (weakly polar) in a mass ratio of 4:4:2.

[0034] (3) Add 0.05 mol / L citrate buffer and chitosan to the concentrated eluent, stir well to obtain a complexing solution, adjust the conductivity of the complexing solution to 80 μS / cm with water at 25℃, cool to 10℃, keep warm for 4 h, filter, wash with 5 wt% ethanol solution, and dry to obtain mangosteen extract. The volume ratio of the concentrated eluent to the citrate buffer is 1:2; the mass ratio of the concentrated eluent to chitosan is 1:0.001.

[0035] Comparative Example 1 The difference between this comparative example and Example 1 is that depressurized ultrasonic extraction was not used.

[0036] A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Dry and pulverize the mangosteen peel to 100 mesh to obtain mangosteen peel powder; Mangosteen peel powder, cysteine, and citric acid are added to water; the mass ratio of mangosteen peel powder, cysteine, and citric acid is 1:0.0008:0.0015; the solid-liquid ratio of mangosteen peel powder to water is 1g:8mL.

[0037] (2) Adjust the pH of the filtrate to 4.5 with sodium citrate, add the compound enzyme and perform enzymatic hydrolysis at 55℃ for 2 hours, inactivate the enzyme at 85℃ for 8 minutes, filter, and concentrate to 10% of the original volume to obtain a concentrated solution; the mass ratio of the filtrate to the compound enzyme is 1:0.003. The compound enzyme includes cellulase, pectinase, arabinogalactanase and xylanase in a mass ratio of 5:5:2:1.

[0038] Add ethanol solution to the concentrate until the ethanol concentration is 35%, and feed it onto the macroporous adsorption resin at a rate of 1.5 BV / h. First, elute with 3 BV of water, then with 3 BV of 25 wt% ethanol solution, then with 3 BV of 60 wt% ethanol solution, and finally with 3 BV of 85 wt% ethanol solution. Collect the eluent, concentrate it, and obtain the concentrated eluent. The macroporous adsorption resins include HZ818 macroporous adsorption resin (non-polar), HZ816 macroporous adsorption resin (non-polar), and HZ841 macroporous adsorption resin (weakly polar) in a mass ratio of 4:4:2.

[0039] (3) Add 0.05 mol / L citrate buffer and chitosan to the concentrated eluent, stir well to obtain a complexing solution, adjust the conductivity of the complexing solution to 80 μS / cm with water at 25℃, cool to 10℃, keep warm for 4 h, filter, wash with 5 wt% ethanol solution, and dry to obtain mangosteen extract. The volume ratio of the concentrated eluent to the citrate buffer is 1:2; the mass ratio of the concentrated eluent to chitosan is 1:0.001.

[0040] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not involve enzymatic hydrolysis.

[0041] A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Dry and pulverize the mangosteen peel to 100 mesh to obtain mangosteen peel powder; Mangosteen peel powder, cysteine, and citric acid were added to a solvent and subjected to reduced pressure ultrasonic extraction. The mixture was then filtered to obtain a filtrate, which was concentrated until no ethanol was present. The mass ratio of mangosteen peel powder, cysteine, and citric acid was 1:0.0008:0.0015. The solid-liquid ratio of mangosteen peel powder to water was 1 g:8 mL, and the solvent was 15% ethanol.

[0042] (2) Add the filtrate to an ethanol solution until the ethanol concentration is 35% to obtain the sample loading solution; The sample solution was loaded onto a macroporous adsorption resin at a rate of 1.5 BV / h. It was first eluted with 3 BV of water, then with 3 BV of 25 wt% ethanol solution, then with 3 BV of 60 wt% ethanol solution, and finally with 3 BV of 85 wt% ethanol solution. The eluent was collected, concentrated, and the concentrated eluent was obtained. The macroporous adsorption resins include HZ818 macroporous adsorption resin (non-polar), HZ816 macroporous adsorption resin (non-polar), and HZ841 macroporous adsorption resin (weakly polar) in a mass ratio of 4:4:2.

[0043] (3) Add 0.05 mol / L citrate buffer and chitosan to the concentrated eluent, stir well to obtain a complexing solution, adjust the conductivity of the complexing solution to 80 μS / cm with water at 25℃, cool to 10℃, keep warm for 4 h, filter, wash with 5 wt% ethanol solution, and dry to obtain mangosteen extract. The volume ratio of the concentrated eluent to the citrate buffer is 1:2; the mass ratio of the concentrated eluent to chitosan is 1:0.001.

[0044] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 is not treated with macroporous adsorption resin.

[0045] A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Dry and pulverize the mangosteen peel to 100 mesh to obtain mangosteen peel powder; Mangosteen peel powder, cysteine, and citric acid were added to a solvent and subjected to reduced pressure ultrasonic extraction. The mixture was then filtered to obtain a filtrate, which was concentrated until no ethanol was present. The mass ratio of mangosteen peel powder, cysteine, and citric acid was 1:0.0008:0.0015. The solid-liquid ratio of mangosteen peel powder to water was 1 g:8 mL, and the solvent was 15% ethanol.

[0046] (2) Adjust the pH of the filtrate to 4.5 with sodium citrate, add the compound enzyme and perform enzymatic hydrolysis at 55℃ for 2 hours, inactivate the enzyme at 85℃ for 8 minutes, filter, and concentrate to 10% of the original volume to obtain a concentrated solution; the mass ratio of the filtrate to the compound enzyme is 1:0.003. The compound enzyme includes cellulase, pectinase, arabinogalactanase and xylanase in a mass ratio of 5:5:2:1.

[0047] (3) Add 0.05 mol / L citrate buffer and chitosan to the concentrate, stir evenly to obtain a complex solution, adjust the conductivity of the complex solution to 80 μs / cm with water at 25℃, cool to 10℃, keep warm for 4 h, filter, wash with 5 wt% ethanol solution, and dry to obtain mangosteen extract. The volume ratio of the concentrate to citrate buffer is 1:2; the mass ratio of the concentrate to chitosan is 1:0.001.

[0048] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 did not undergo complexation crystallization treatment.

[0049] A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Dry and pulverize the mangosteen peel to 100 mesh to obtain mangosteen peel powder; Mangosteen peel powder, cysteine, and citric acid were added to a solvent and subjected to reduced pressure ultrasonic extraction. The mixture was then filtered to obtain a filtrate, which was concentrated until no ethanol was present. The mass ratio of mangosteen peel powder, cysteine, and citric acid was 1:0.0008:0.0015. The solid-liquid ratio of mangosteen peel powder to water was 1 g:8 mL, and the solvent was 15% ethanol.

[0050] (2) Adjust the pH of the filtrate to 4.5 with sodium citrate, add the compound enzyme and perform enzymatic hydrolysis at 55℃ for 2 hours, inactivate the enzyme at 85℃ for 8 minutes, filter, and concentrate to 10% of the original volume to obtain a concentrated solution; the mass ratio of the filtrate to the compound enzyme is 1:0.003. The compound enzyme includes cellulase, pectinase, arabinogalactanase and xylanase in a mass ratio of 5:5:2:1.

[0051] Add ethanol solution to the concentrate until the ethanol concentration is 35%, and feed it onto the macroporous adsorption resin at a rate of 1.5 BV / h. First, elute with 3 BV of water, then with 3 BV of 25 wt% ethanol solution, then with 3 BV of 60 wt% ethanol solution, and finally with 3 BV of 85 wt% ethanol solution. Collect the eluent, concentrate it, and obtain the concentrated eluent. The macroporous adsorption resins include HZ818 macroporous adsorption resin (non-polar), HZ816 macroporous adsorption resin (non-polar), and HZ841 macroporous adsorption resin (weakly polar) in a mass ratio of 4:4:2.

[0052] (3) Dry the concentrated eluent to obtain mangosteen extract.

[0053] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that ethanol hot extraction was used instead of reduced pressure ultrasonic extraction.

[0054] A method for preparing a mangosteen extract with intimate care functions includes the following steps: (1) Dry and pulverize the mangosteen peel to 100 mesh to obtain mangosteen peel powder; Mangosteen peel powder was extracted with 70wt% ethanol solution at 60℃ for 60min. The solid-liquid ratio of mangosteen peel powder to ethanol solution was 1g:6mL. The mixture was filtered and concentrated until no ethanol was found in the filtrate to obtain a concentrated solution. (2) Adjust the pH of the concentrate to 4.5 with sodium citrate, add the compound enzyme and perform enzymatic hydrolysis at 55℃ for 2 hours, inactivate the enzyme at 85℃ for 8 minutes, filter, and concentrate to 10% of the original volume to obtain the concentrate; the mass ratio of the filtrate to the compound enzyme is 1:0.003. The compound enzyme includes cellulase, pectinase, arabinogalactanase and xylanase in a mass ratio of 5:5:2:1.

[0055] Ethanol solution was added to the concentrate until the ethanol concentration was 35%. The concentrate was fed onto a macroporous adsorption resin at a rate of 1.5 BV / h. The solution was first eluted with 3 BV of water, then with 3 BV of 25 wt% ethanol solution, then with 3 BV of 60 wt% ethanol solution, and finally with 3 BV of 85 wt% ethanol solution. The eluent was collected and concentrated to obtain the concentrated eluent. The macroporous adsorption resins include HZ818 macroporous adsorption resin (non-polar), HZ816 macroporous adsorption resin (non-polar), and HZ841 macroporous adsorption resin (weakly polar) in a mass ratio of 4:4:2.

[0056] (3) Add 0.05 mol / L buffer solution and chitosan to the concentrated eluent, stir well to obtain a complexing solution, adjust the conductivity of the complexing solution to 80 μS / cm with water at 25℃, cool to 10℃, keep warm for 4 h, filter, rinse with 5 wt% ethanol solution, and dry to obtain mangosteen extract. The volume ratio of the concentrated eluent to the buffer solution is 1:2; the mass ratio of the concentrated eluent to chitosan is 1:0.001.

[0057] To further demonstrate the effectiveness of the present invention, the following test methods are provided: 1. The content of α-dextrin in mangosteen extract was determined by HPLC. The HPLC method for the determination of α-dextrin in mangosteen extract was as follows: methanol:water = 85:15, detection wavelength 363 nm, flow rate 1 ml / min, column temperature 40 ℃, and injection volume 10 μl.

[0058] The spectra of Example 1 and Comparative Examples 1-5 are as follows: Figures 1-6 As shown.

[0059] 2. Mix mangosteen extract, water, and butylene glycol at a ratio of 1:19:80 to obtain the test sample.

[0060] Specific testing methods: The bacterial strains stored in the -80℃ bacterial culture bank were taken out, activated, and single colonies were picked and inoculated into the culture medium, and cultured until the viable count reached 1×10⁻⁶. 9 CFU / mL, then the bacterial culture was diluted with PBS to a viable count of 4 × 10⁻⁶. 4 The bacterial solution with a concentration of CFU / mL is used to prepare the inoculation solution.

[0061] Add 0.1 mL PBS and 4.9 mL inoculated bacterial solution to the control tube, and add 0.1 mL sample and 4.9 mL inoculated bacterial solution to the sample tube. After vortexing and mixing, perform 10-fold serial dilutions. Pipette 1 mL of the sample solution and diluent into a Petri dish, pour in the culture medium, rotate the dish to ensure thorough mixing, and invert the plate after the agar has solidified. Incubate at 37°C for 48 h. Select plates with appropriate dilutions and colony counts between 100 CFU for viable colony counting. Inhibition rate (%) = (average colony count of control sample × dilution factor - average colony count of test sample × dilution factor) / average colony count of control sample × dilution factor × 100.

[0062] Table 1

[0063] As can be seen from Table 1, the mangosteen extract described in this invention has the effects of cleaning and inhibiting bacteria, regulating vaginal flora imbalance, thereby reducing the occurrence of inflammatory reactions and improving the vagina's self-cleaning ability.

[0064] Based on the above-described preferred embodiments of the present invention, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the technical spirit of the present invention. The technical scope of the present invention is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A method for preparing mangosteen extract with intimate care function, characterized in that, Includes the following steps: (1) Add mangosteen peel powder, cysteine ​​and citric acid to the solvent, perform vacuum ultrasonic extraction, filter, obtain filtrate, and concentrate until the filtrate is free of ethanol; (2) Adjust the pH of the filtrate to 4.2-4.8 with sodium citrate, add a compound enzyme for enzymatic hydrolysis, inactivate the enzyme, filter, concentrate to obtain a concentrated solution, add ethanol solution, and load the solution onto a macroporous adsorption resin at 1-2 BV / h. First, elute with 2-4 BV of water, then elute with 2-4 BV of 20-30 wt% ethanol solution, then elute with 3-4 BV of 50-60 wt% ethanol solution, and finally elute with 2-4 BV of 80-90 wt% ethanol solution. Collect the eluent, concentrate it, and obtain a concentrated eluent. (3) Add citrate buffer and chitosan to the concentrated eluent, stir evenly to obtain a complex solution, adjust the conductivity of the complex solution to 78~82μs / cm with water at 20~30℃, crystallize by gradient cooling, filter, wash, and dry to obtain mangosteen extract.

2. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The mass ratio of mangosteen peel powder, cysteine, and citric acid is 1:(0.0005~0.002):(0.001~0.002).

3. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The solid-liquid ratio of the mangosteen peel powder and the solvent is 1g:(6~10)mL, and the solvent is 10%~20% ethanol.

4. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The vacuum ultrasonic extraction was performed at a temperature of 40-50°C, an ultrasonic power of 400W, a vacuum degree of -0.08 MPa, and a time of 30 minutes.

5. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The mass ratio of the filtrate to the compound enzyme is 1:(0.002~0.004).

6. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The complex enzyme comprises cellulase, pectinase, arabinogalactase and xylanase in a mass ratio of (4~6):(4~6):(1~3):(0.5~2).

7. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The enzymatic hydrolysis temperature is 50~58℃, and the time is 1~4h; The enzyme inactivation temperature is 80~90℃, and the time is 6~12min.

8. The method for preparing mangosteen extract with intimate care function according to claim 1, characterized in that, The volume ratio of the concentrated eluent to the citrate buffer is 1:(2~5). The mass ratio of the concentrated eluent to chitosan is 1:(0.0005~0.002). The molar concentration of the citrate buffer solution is 0.02~0.1 mol / L; The macroporous adsorption resin includes HZ818 macroporous adsorption resin, HZ816 macroporous adsorption resin and HZ841 macroporous adsorption resin in a mass ratio of (3~5):(3~5):(1~3).

9. A mangosteen extract with intimate care function, characterized in that, It is prepared by any of the preparation methods described in claims 1 to 8.

10. The use of the mangosteen extract with intimate care function as described in claim 9 in the preparation of cosmetics.

Images