Oral care agent containing acidic electrolyzed oxidizing water as well as preparation method and application of oral care agent

Abstract

The invention relates to the technical field of medical care materials, discloses an oral care agent containing acidic electrolyzed oxidizing water as well as a preparation method and application of the oral care agent, and aims to solve the problem that available chlorine and oxidation-reduction potential of the acidic electrolyzed oxidizing water are extremely easy to attenuate in the storage process of the acidic electrolyzed oxidizing water. The preparation method comprises the following steps: pre-dispersing chitosan and a part of glycerol, sequentially mixing with other components, homogenizing, adjusting the pH value, curing, degerming and filling, so as to obtain the high-stability and high-stability chitosan stabilizer through the synergistic effect of the components, thereby obtaining the high-stability and high-stability chitosan stabilizer. According to the present invention, the weak acid environment with the optimal sterilization effect is maintained while the chemical stability and the antibacterial function durability of the product are improved, the preparation process is simple and convenient, the property is mild, the mucous membrane repair potential is provided, and the product is not only suitable for daily oral cavity cleaning and nursing, but also can be used for auxiliary nursing of body surface mucous membranes and slight wound surfaces.

Description

Technical Field

[0001] This invention relates to the field of medical care materials technology, and in particular to an oral care agent containing acidic oxidizing potential water, its preparation method and application. Background Technology

[0002] Oxidized electrolyzed water, especially its active ingredient hypochlorous acid (HClO), has attracted widespread attention in the fields of medical disinfection and hygiene care due to its broad-spectrum, high-efficiency, and non-inducible antimicrobial resistance bactericidal properties. The bactericidal activity of hypochlorous acid molecules is closely related to their form. Hypochlorous acid molecules have the highest proportion in the pH range of 5.5-7.0. Therefore, the development of weakly acidic oxidized electrolyzed water products is of great significance for applications requiring high safety and high efficiency, such as oral care and wound irrigation. However, hypochlorous acid molecules are chemically extremely reactive in a weakly acidic environment, which causes the effective chlorine concentration and redox potential, which are mainly composed of hypochlorous acid, to decay rapidly during storage, resulting in extremely poor product stability.

[0003] Existing technologies have proposed various solutions. For example, the "Preparation method of oral antibacterial solution based on slightly acidic oxidizing potential water" published in CN118854303A involves adding a composite stabilizer containing polyhexamethylene biguanide, metal ion chelating agent and phosphate to slightly acidic oxidizing potential water. Its stabilization mechanism mainly relies on the broad-spectrum effect of the added stabilizer, and its inhibitory effect on the rapid decomposition pathway dominated by the high activity of hypochlorous acid is limited. The "Mouthwash or Toothwash Composition with Stable Chlorine Dioxide and Inorganic Stabilizer Phosphate" published in CN111494221A uses monofluorophosphate to prevent chlorine dioxide gas from escaping from the composition, thereby extending the shelf life. However, the chemical properties and instability mechanisms of chlorine dioxide and hypochlorous acid water are completely different, and this stabilization strategy cannot effectively solve the problem of maintaining the reduction potential and available chlorine in acidic oxidizing potential water.

[0004] Meanwhile, in the field of medical dressings and nursing materials, liquid or gel preparations that come into direct contact with the body surface mucosa or wounds are required to have not only long-lasting antibacterial stability, but also higher requirements for biocompatibility, mildness, and tissue repair-promoting functions. Summary of the Invention

[0005] The technical problem to be solved by this invention is: how to provide a liquid oral mucosa care agent that is simple to prepare, uses mild materials, and can maintain a high effective chlorine retention rate and redox potential stability for a long time. To this end, we propose an oral care agent containing acidic oxidizing potential water, its preparation method and application.

[0006] To achieve the above objectives, this application adopts the following technical solution: an oral care agent containing acidic oxidizing potential water, comprising weakly acidic oxidizing potential water as an active ingredient, having a pH value of 6.0-7.0, and a composite stabilizer system for stabilizing the weakly acidic oxidizing potential water, the composite stabilizer system being composed of a phosphate buffer, a small molecule amino compound, an anionic surfactant, and chitosan.

[0007] Preferably, the content of the weakly acidic oxidizing potential water is 85.0%-92.0%, the oxidation-reduction potential of the weakly acidic oxidizing potential water is ≥800mV, and the effective chlorine concentration is 80-120mg / L.

[0008] Preferably, the contents of each component in the composite stabilizer system are 1.0%-1.5% phosphate buffer, 1.0%-1.5% small molecule amino compound, 0.2%-0.6% anionic surfactant, and 1.0%-1.5% chitosan.

[0009] Preferably, the phosphate buffer is composed of sodium dihydrogen phosphate and disodium hydrogen phosphate.

[0010] Preferably, the small molecule amino compound is selected from one or more of urea, acetamide, glycine and its hydrochloride.

[0011] Preferably, the anionic surfactant is selected from one or more of sodium dodecyl sulfonate and sodium dodecyl sulfate.

[0012] Preferably, the degree of deacetylation of the chitosan is ≥85%, and the viscosity is <100 mPa·s.

[0013] Preferably, the oral care agent further includes 4.0%-6.0% by weight of the moisturizing agent glycerin, and the oral care agent has a pH value of 6.0-7.0.

[0014] A method for preparing an oral care agent containing acidic electrolyzed water includes the following steps: S1: Chitosan and a portion of glycerol are mixed and ground to form a pre-dispersion; S2: Weakly acidic electrolyzed water is mixed and dissolved with a phosphate buffer and a small molecule amino compound to obtain a main solution; S3: An anionic surfactant is added to the main solution and stirred to disperse; S4: The pre-dispersion is added, and the remaining glycerol is added, and the mixture is stirred to homogenize and form a mixture; S5: The pH of the mixture is adjusted to 6.0-7.0 and allowed to stand for aging; S6: The aged solution is filtered for sterilization and then filled to obtain the finished product.

[0015] The application of an oral care agent containing acidic oxidizing electrolyzed water in the preparation of products for cleaning, disinfecting or caring for the oral cavity and body surface mucosa.

[0016] The technical effects and advantages of this invention are as follows: In this invention, a phosphate buffer adapted to a weakly acidic environment is used to precisely stabilize the pH of the system within the range of 6.0-7.0, where hypochlorous acid has the highest proportion, to achieve optimal sterilization. Small molecule amino compounds generate chloramine derivatives in situ under this environment, and anionic surfactants achieve interfacial dispersion of active molecules. At the same time, chitosan chelates trace catalytic metal ions. The components complement each other and work synergistically under acidic conditions. With highly active and high-proportion hypochlorous acid molecules as the basis for immediate sterilization, the above-mentioned multi-target and multi-mechanism composite stabilization strategy inhibits various decomposition pathways during the storage of weakly acidic oxidizing potential water, thereby maintaining its high redox potential and effective chlorine concentration at the source. While maintaining the inherent high-efficiency sterilization potential of the acidic environment, the antibacterial function is effectively maintained for a long time. At the same time, the product also has good mucosal affinity and auxiliary repair potential. The preparation process is simple and easy to scale up. Attached Figure Description

[0017] The disclosure of this invention is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this invention. In the drawings, the same reference numerals are used to refer to the same parts: Figure 1 This is a flowchart illustrating the preparation process of the oral care agent of the present invention. Figure 2 This is a graph showing the change in the effective chlorine retention rate of the present invention over time. Figure 3 This is a graph showing the decrease in redox potential over time according to the present invention. Figure 4 This is a graph showing the pH value changing over time according to the present invention. Figure 5 This is a graph showing the minimum bactericidal concentration of Staphylococcus aureus according to the present invention as a function of time. Figure 6 This is a graph showing the minimum bactericidal concentration of Candida albicans according to the present invention as a function of time. Detailed Implementation

[0018] It is readily understood that, based on the technical solution of this invention, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of the invention. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative examples of the technical solution of this invention and should not be considered as the entirety of the invention or as limitations or restrictions on the technical solution of this invention.

[0019] This invention provides an oral care agent containing acidic electrolyzed water. The agent is a liquid composition, the core of which is to use weakly acidic electrolyzed water with specific physicochemical properties as the active base, and to synergistically stabilize and enhance its function through an original composite stabilizer system. This improves its chemical stability while maintaining its high bactericidal potential and mucosal affinity. This agent is not only suitable for daily oral care, but its gentle properties and repair function also make it suitable for the auxiliary care of skin mucosa and minor wounds where safety requirements are higher.

[0020] The composition of oral care products, by weight percentage, is shown in Table 1 below.

[0021]

[0022] Table 1 All raw materials used in this invention are commercially available industrial-grade, food-grade, or pharmaceutical-grade products. Those skilled in the art can select equivalent raw materials based on production scale and quality standards.

[0023] The technical effect of this invention depends on the unique function of each component in a weakly acidic environment and the synergistic effect they produce, rather than the simple sum of the functions of each component.

[0024] The primary destabilizing factor for weakly acidic electrolyzed water is pH drift, caused by hypochlorous acid (HClO) or hypochlorite ions (ClO). - The distribution ratio of HClO is highly dependent on pH value; any fluctuation in pH will cause HClO to convert to ClO. - Transformation, or triggering other decomposition pathways, can weaken the bactericidal efficacy.

[0025] In this invention, the phosphate buffer is specifically composed of sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen tripolyphosphate (Na2HPO4), wherein the purity of NaH2PO4 is ≥99.0% and the purity of Na2HPO4 is ≥95.0%. The second-order dissociation constant pKa2 of the sodium dihydrogen phosphate and disodium hydrogen phosphate buffer system is approximately 7.2, providing buffering capacity within the target pH range to resist H2 dissociation caused by fluctuations in raw materials, absorption of CO2 from the air, or trace reactions during storage. + The concentration changes, thereby locking the pH value within a preset range.

[0026] With pH stabilized, the next key step is to address the problem of rapid consumption caused by the high reactivity of hypochlorous acid molecules.

[0027] In this invention, a small molecule amino compound, serving as a chloramine precursor and stabilizing synergist, is selected from one or more of urea, acetamide, glycine, and their hydrochlorides. Under stable weakly acidic conditions, the amino (-NH2) or amide (-CONH2) groups carried by small molecule amino compounds react with hypochlorous acid (HClO) to generate chloramine (NH2Cl) derivatives in situ. First, it should be noted that the generated chloramine itself has mild and long-lasting antibacterial activity. Second, and more importantly, chloramine acts as an active chlorine reserve in the system. When free hypochlorous acid (HClO) is consumed due to its immediate bactericidal function or non-targeted decomposition, the chloramine (NH2Cl) derivative releases HClO again, thus converting a portion of the unstable and easily dissipated active chlorine into a relatively stable and slowly released reserve form, thereby significantly slowing down the decay rate of the total effective chlorine concentration.

[0028] In this invention, the anionic surfactant, as a charge and interface stabilizer, is selected from one or more of sodium dodecyl sulfonate and sodium dodecyl sulfate; In a weakly acidic medium, surfactant molecules interact with polar reactive substances such as HClO and chloramine derivatives in the solution through their hydrophobic chains, generating weak van der Waals forces to form a dynamic and reversible encapsulation state. This means that a dynamic steric hindrance layer is constructed around the active molecules, which can prevent direct collisions and aggregation between these highly reactive substances, thereby inhibiting the autocatalytic decomposition or mutual deactivation reactions caused by them, and allowing the active ingredients to exist in a highly dispersed and stable state in the homogeneous system. In addition, the introduction of surfactants reduces the surface tension of the system and enhances the wettability and spreading ability of the solution on biological surfaces such as oral mucosa. This allows the care solution to cover the mucosal folds or biofilm surface more quickly and evenly, forming a thinner liquid film, prolonging the residence time of the active ingredients at the site of action, and improving bioavailability.

[0029] Chitosan acts as a chemical stabilizer in this system. The amino groups abundant in its molecular chain can be partially protonated, forming positively charged centers that can control trace amounts of Fe in the solution. 2+ Cu 2+ Transition metal ions have extremely strong chelating and immobilizing abilities. These metal ions are common and highly efficient catalysts for the decomposition of hypochlorous acid. Chitosan mitigates the accelerated decay of available chlorine by capturing and isolating them on the polymer chain.

[0030] Glycerin has strong hygroscopic and moisturizing properties. Its addition can reduce the water activity of liquid systems. Since the rates of almost all unstable chemical reactions, such as hypochlorous acid decomposition, free radical chain reactions, and metal ion catalysis, are positively correlated with water activity, the addition of glycerin can generally slow down the process of the above-mentioned adverse reactions from a physicochemical perspective, providing basic support for chemically stable systems. In the preparation process, glycerol is crucial for achieving uniform dissolution of chitosan. If chitosan powder is directly added to the aqueous phase, it is very easy to agglomerate due to hydrogen bonding and is difficult to dissolve. By pre-mixing and grinding it with glycerol, glycerol molecules can be inserted between chitosan particles, preventing uncontrollable agglomeration after contact with water through steric hindrance and hydrogen bonding.

[0031] Reference Figure 1 As shown, the present invention also provides a method for preparing an oral care agent containing acidic electrolyzed water, which is used to prepare the above-mentioned oral care agent, specifically including the following steps: S1: Take half of the formula amount of glycerol and mix it with all the chitosan in a dry container. Grind and stir until a uniform paste without visible particles is formed to obtain the chitosan predispersant.

[0032] S2: Place the prescribed amount of acidic oxidizing potential water in a corrosion-resistant preparation tank, and add sodium dihydrogen phosphate and disodium hydrogen phosphate sequentially at a stirring rate of 200-4000 r / min. Stir until completely dissolved, then add the small molecule amino compound and continue stirring until dissolved to obtain a clear main solution.

[0033] S3: Keep stirring and add anionic surfactant to the main solution. Continue stirring for 20-30 minutes to ensure that the surfactant is fully dispersed and dissolved. The solution should remain clear and transparent.

[0034] S4: Add the chitosan predispersant obtained in S1 to the preparation tank, and wash the container with the remaining glycerol and add it in. Increase the stirring speed to 400-500 r / min and continue stirring for 50-60 min to completely dissolve the chitosan and mix it evenly with other components to form a homogeneous and stable mixture.

[0035] S5: Add deionized water to the mixture until the total weight of the formula is reached, stir well, adjust the pH of the system to 6.0-7.0 with dilute citric acid solution or dilute sodium bicarbonate solution, transfer the adjusted solution to a sealed, light-proof container, and let it stand at room temperature for 24 hours to mature.

[0036] S6: The matured solution is filtered through a microporous membrane with a pore size of 0.22μm for sterilization, and then filled into an opaque, sealed container to obtain the finished oral care agent.

[0037] The nursing composition prepared by the above method is stable and has both broad-spectrum and rapid bactericidal effects and good mucosal affinity.

[0038] This care product can be used directly as a mouthwash or oral rinse, or as an active ingredient in the preparation of wipes, gels, or medical dressings suitable for skin and mucous membrane care.

[0039] To further verify the technical effects of the nursing composition of the present invention, the present invention will be further described in detail below through specific embodiments and comparative examples. These examples are used to illustrate the present invention, and are not intended to limit its scope of protection.

[0040] Unless otherwise specified, all embodiments and comparative examples are prepared according to the general process. The acidic oxidizing potential water mentioned in each example refers to the raw material that meets the requirements of the present invention, with an initial pH of 6.5, an oxidation-reduction potential (ORP) of 850mV, and an effective chlorine (ACC) concentration of 100mg / L.

[0041] Example 1 This embodiment provides an oral care agent containing acidic electrolyzed water. The main components, by weight, include 88.0% weakly acidic electrolyzed water, 0.4% sodium dihydrogen phosphate, 0.8% disodium hydrogen phosphate, 1.2% urea, 0.3% sodium dodecyl sulfate, 1.0% chitosan, 5.0% glycerin, and the remainder is deionized water.

[0042] This embodiment also provides a method for preparing an oral care agent containing acidic electrolyzed water, which is carried out according to the aforementioned preparation method of the present invention, specifically as follows: First, chitosan and 2.5% glycerol are ground to form a pre-dispersed paste. Oxidized potential water is placed in a preparation tank, and sodium dihydrogen phosphate, disodium hydrogen phosphate, and urea are added in sequence and stirred to dissolve. Sodium dodecyl sulfonate is added and stirred until clear. The chitosan pre-dispersed paste and the remaining glycerol are added and stirred at high speed to homogenize. After adding water to make up the volume, the pH is adjusted to 6.5 with dilute citric acid solution. After aging in a sealed container for 24 hours, the product is filtered through a 0.22μm filter membrane and filled into bottles to obtain a clear and transparent finished product.

[0043] Example 2 This embodiment provides an oral care agent containing acidic oxidizing potential water. Except for adjusting the amount of sodium dodecyl sulfonate to 0.2%, the other components, amounts, and preparation processes are the same as in Example 1.

[0044] Example 3 This embodiment provides an oral care agent containing acidic oxidizing potential water. Except for adjusting the amount of sodium dodecyl sulfate to 0.6% and the amount of chitosan to 1.5%, the other components, amounts, and preparation process are the same as in Example 1.

[0045] Example 4 This embodiment provides an oral care agent containing acidic electrolyzed water. Except for adjusting the amount of urea to 1.0% and the amount of glycerin to 4.0%, the other components, amounts, and preparation process are the same as in Example 1.

[0046] Example 5 This embodiment provides an oral care agent containing acidic oxidized electrolyzed water. Except for replacing urea with glycine hydrochloride and adjusting its addition amount to 1.07% based on an equal molar amount of amino, the other components, dosages, and preparation processes are the same as in Example 1.

[0047] Example 6 This embodiment provides an oral care agent containing acidic oxidizing potential water. Except for adjusting the amount of sodium dihydrogen phosphate to 0.3%, the amount of disodium hydrogen phosphate to 0.7%, and using a dilute sodium bicarbonate solution to adjust the final pH to 6.2, the other components, amounts, and preparation process are the same as in Example 1.

[0048] Example 7 This embodiment provides an oral care agent containing acidic oxidizing potential water. Except for replacing the surfactant with sodium dodecyl sulfonate at a dosage of 0.25%, the other components, dosages, and preparation processes are the same as in Example 1.

[0049] Example 8 This embodiment provides an oral care agent containing acidic oxidizing potential water. Except for adjusting the amount of acidic oxidizing potential water to 90.0% and reducing the amount of deionized water accordingly, the other components, amounts, and preparation processes are the same as in Example 1.

[0050] Comparative Example 1 The difference between this comparative example and Example 1 is that chitosan is not added in the formulation and preparation process, the chitosan pre-dispersion step is omitted during preparation, and all the glycerol in the formulation is added in the main mixing stage.

[0051] Comparative Example 2 The difference between this comparative example and Example 1 is that urea is not added in the formulation and preparation process.

[0052] Comparative Example 3 The difference between this comparative example and Example 1 is that no anionic surfactant is added in the formulation and preparation process.

[0053] Comparative Example 4 The difference between this comparative example and Example 1 is that the phosphate buffer in the composite stabilizer is replaced with 0.25% sodium dihydrogen phosphate and 1.0% sodium tripolyphosphate.

[0054] Comparative Example 5 This comparative example refers to the method disclosed in the example of prior art CN118854303A to prepare a slightly acidic oxidizing potential water antibacterial solution with a pH of approximately 6.0; Specifically, after electrolyzing the sodium chloride solution, the resulting acidic water is mixed with alkaline water to adjust to a slightly acidic state, and the self-made stabilizer and peppermint extract described in the document are added. The mixture is then filtered and bottled using the same steps.

[0055] Comparative Example 6 This comparative example directly uses the same weakly acidic oxidizing potential water stock solution from the same source and batch as Example 1, without any form of additive compounding or stabilization treatment.

[0056] To objectively verify the effectiveness of the oral care agent of the present invention, clarify the performance differences among the various embodiments and comparative examples, and highlight the technical advantages of the present invention, the finished products prepared in Examples 1-8 and Comparative Examples 1-6 were selected for performance testing. The specific test plan is as follows: Test Example 1 An accelerated testing method was used. Samples from each example and comparative example were placed in a 40°C incubator and stored in the dark. Samples were taken on days 0, 15, 30, 45, 60, 75, and 90 to determine the available chlorine retention rate, the decrease in redox potential, and the pH value, as detailed below: The effective chlorine concentration was determined by titration with sodium thiosulfate standard solution, and the retention rate of ACC at each time point relative to day 0 was calculated. The results are shown in [Table missing]. Figure 2 As shown; redox potentials were measured using a calibrated platinum electrode ORP meter, and the decrease at each time point relative to day 0 was calculated. The results are shown in [Figure number missing]. Figure 3 As shown; pH values ​​were measured using a pH meter, and the results are shown in [the image / data]. Figure 4 As shown.

[0057] according to Figure 2 Data shows that the ACC retention rate of Examples 1-8 using the complete composite stabilizer system of the present invention showed a slow and steady downward trend throughout the 90-day test period. Among them, Example 1 maintained an ACC retention rate of 86.5% on the 90th day, showing the best stability. The slope of the ACC decay curves in Comparative Examples 1-4 increased significantly, and the retention rate on day 90 was much lower than that in Example 1. This comparative result proves that the composite system composed of phosphate buffer, small molecule amino compound, anionic surfactant and chitosan used in this invention plays an indispensable synergistic role in stabilizing ACC. The absence of any single component will lead to a significant decrease in stability. The ACC of Comparative Example 6 showed a sharp decline, with a retention rate of only 33.5% on day 90, demonstrating the inherent extreme instability of acidic oxidized potential water without stabilization treatment.

[0058] according to Figure 3 The data shows that the ORP decline trend in Examples 1-8 is gradual, and... Figure 2The slow decay pattern of ACC in the samples was highly consistent, indicating that its oxidation capacity was effectively maintained. The ORP of Comparative Examples 1-4 decreased significantly, with a decrease of 99-134 mV on day 90, which was much greater than that of the other examples. This once again confirms that the absence of any stabilizing component will destroy the overall stability mechanism of the system, leading to a rapid loss of oxidation potential.

[0059] according to Figure 4 The data shows that the pH curves of Examples 1-8 are the most stable, consistently remaining within the target range of 6.0-7.0. This is attributed to the efficient regulation of the sodium dihydrogen phosphate-disodium hydrogen phosphate buffer pair. A stable pH environment is a prerequisite and foundation for maintaining the dominance of hypochlorous acid and ensuring the long-term stability of ACC and ORP. Comparative Example 4 uses a sodium dihydrogen phosphate-sodium tripolyphosphate buffer system. Sodium tripolyphosphate gradually hydrolyzes in a weakly acidic or near-neutral environment, eventually forming orthophosphate. This hydrolysis process consumes hydrogen ions in the water, leading to an increase in the pH value of the solution. At the same time, since sodium tripolyphosphate itself is alkaline, the initial pH of the system is even higher when it is prepared. Comparative Example 6 completely lacks buffer components, and the acid produced by the decomposition of hypochlorous acid will directly lead to an increase in the hydrogen ion concentration. The pH should show a rapid and significant decrease, demonstrating the importance of the buffer system being adapted to the target pH environment.

[0060] Test Example 2 Referring to the test principle of GB / T 21510-2008 "Test Method for Antibacterial Properties of Nano-Inorganic Materials", and with adaptive adjustments made for the characteristics of liquid samples, two standard oral-associated pathogenic microorganisms were selected for the test: Staphylococcus aureus ( S.aureus ), ATCC 6538, as a representative of Gram-positive bacteria; Candida albicans ( C. albicans ), ATCC 10231, as a representative of fungi.

[0061] To clearly demonstrate the impact of the absence of different stable components and the key role of system acidity, representative samples were selected for testing. Samples from Example 1, Comparative Examples 1, 2, 3, and 5 were placed in a constant temperature incubator at 40°C in the dark for accelerated testing. Samples were taken on days 0, 10, 20, 30, 40, 50, 60, 70, 80, and 90 at the start of the test. Due to its inherent extreme instability, Comparative Example 6 was only tested on day 0 as a baseline reference for initial bactericidal efficacy.

[0062] The specific testing method is as follows: The activated test bacteria are inoculated into nutrient broth and cultured at 37°C with shaking until the logarithmic growth phase. The bacterial concentration is then adjusted to approximately 1×10⁻⁶ using phosphate buffer. 6 -5×10 6CFU / mL, dilute the sample to be tested with sterile deionized water; take 0.5 mL of bacterial suspension and 0.5 mL of sample solutions of different dilutions and mix them evenly in a sterile test tube to make the final sample concentration in the reaction system half of the preset dilution, and react in a water bath at 20±1℃ for 5 min; after the reaction, immediately add 4.0 mL of neutralizing broth containing 1% sodium thiosulfate, mix well, and stop the sterilization reaction; take 0.1 mL of the mixture and spread it on a nutrient agar plate, making two replicates for each dilution, and incubate upside down at 37℃ for 48 h; count the number of colonies on the plate and calculate the kill rate, where the test results for Staphylococcus aureus are shown in […]. Figure 5 As shown, the results of the Candida albicans test are as follows: Figure 6 As shown.

[0063] according to Figure 5-6 The data showed that the MBC of Example 1 showed a slow and continuous upward trend over time, with the MBC against Staphylococcus aureus increasing from 3.13% to 14.0%. This indicates that although its antibacterial efficacy decreased, it remained within the effective concentration range that could be used throughout the entire 90-day accelerated test period. This directly corresponds to the slow decrease of its ACC in Test Example 1, confirming that the composite stable system effectively maintained the long-term biological activity of the product.

[0064] In contrast, the slope of the MBC rising curves of Comparative Examples 1, 2, and 3 was significantly steeper than that of Example 1. In the later stages of storage, even when using undiluted stock solutions of these comparative examples, their bactericidal effect could not meet the standard requirements. Figure 5 and Figure 6 The study did not demonstrate that chitosan, small molecule amino compounds, and anionic surfactants all play an indispensable synergistic role in maintaining the long-lasting antibacterial properties of the product, and the absence of any one of them would lead to a significant reduction in the functional lifespan.

[0065] Throughout the testing period, the MBC value of Comparative Example 5 was consistently much higher than that of Example 1 during the same period, confirming that within the more precise optimal range of weak acidity determined by this invention, a higher proportion of hypochlorous acid molecules maintained by the composite stabilization system can provide a more advantageous starting point and persistence of bactericidal efficacy.

[0066] The technical scope of this invention is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this invention, and all such modifications and variations should fall within the protection scope of this invention.

Claims

1. An oral care agent containing acidic electrolyzed water, characterized in that, It includes weakly acidic oxidizing potential water as an active ingredient, with a pH value of 6.0-7.0, and a composite stabilizer system for stabilizing the weakly acidic oxidizing potential water, the composite stabilizer system being composed of a phosphate buffer, a small molecule amino compound, an anionic surfactant and chitosan.

2. The oral care agent containing acidic electrolyzed water according to claim 1, characterized in that, The content of the weakly acidic oxidizing potential water is 85.0%-92.0%, the oxidation-reduction potential of the weakly acidic oxidizing potential water is ≥800mV, and the effective chlorine concentration is 80-120mg / L.

3. The oral care agent containing acidic electrolyzed water according to claim 1, characterized in that, The contents of each component in the composite stabilizer system are as follows: phosphate buffer 1.0%-1.5%, small molecule amino compound 1.0%-1.5%, anionic surfactant 0.2%-0.6%, and chitosan 1.0%-1.5%.

4. An oral care agent containing acidic electrolyzed water according to claim 1 or 3, characterized in that, The phosphate buffer is composed of sodium dihydrogen phosphate and disodium hydrogen phosphate.

5. An oral care agent containing acidic electrolyzed water according to claim 1 or 3, characterized in that, The small molecule amino compound is selected from one or more of urea, acetamide, glycine and its hydrochloride.

6. An oral care agent containing acidic electrolyzed water according to claim 1 or 3, characterized in that, The anionic surfactant is selected from one or more of sodium dodecyl sulfonate and sodium dodecyl sulfate.

7. An oral care agent containing acidic electrolyzed water according to claim 1 or 3, characterized in that, The chitosan has a degree of deacetylation ≥85% and a viscosity <100 mPa·s.

8. An oral care agent containing acidic electrolyzed water according to claim 1, characterized in that, The oral care agent also includes 4.0%-6.0% by weight of the moisturizing agent glycerin, and the oral care agent has a pH value of 6.0-7.

0.

9. A method for preparing an oral care agent containing acidic oxidizing potential water as described in any one of claims 1-8, characterized in that, Includes the following steps: S1: Chitosan and a portion of glycerol are mixed and ground to form a pre-dispersion; S2: Acidic oxidizing potential water is mixed and dissolved with phosphate buffer and small molecule amino compound to obtain the main solution; S3: Add anionic surfactant to the main solution and stir to disperse; S4: Add the pre-dispersed mixture and the remaining glycerol, stir to homogenize and form a mixture; S5: Adjust the pH of the mixture to 6.0-7.0 and let it stand to mature; S6: After the matured solution is filtered to remove bacteria and then filled, the finished product is obtained.

10. The use of an oral care agent containing acidic oxidizing potential water as described in any one of claims 1-8 in the preparation of products for cleaning, disinfecting or caring for the oral cavity and body surface mucosa.

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