Method for increasing rhizome germination rate of carex baileyi
Soaking white sedge seedlings in IBA, NAA, and 6-BA hormone solutions solved the problem of low germination rate caused by seed dormancy, achieving a highly efficient and stable increase in rhizome germination rate, which is suitable for the field of lawn and ground cover plant propagation.
Patent Information
- Application Number
- CN202511946166.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-22
- Publication Date
- 2026-02-27
AI Technical Summary
The dormancy of white sedge seeds leads to slow germination, and traditional treatment methods have limited effectiveness. This results in a long waiting period after sowing, susceptibility to weed invasion, drought stress, and soil compaction. Even after high-density emergence, the seedlings grow slowly, making it difficult to apply them on a large scale in landscaping. Furthermore, the operation is extensive and lacks quantitative standards.
White sedge seedlings were soaked in hormone solutions such as IBA, NAA, and 6-BA at a concentration of 20-80 mg/L for 15-60 minutes. Afterward, the roots were washed and trimmed to a specific length, and then placed on a seedbed for germination rate statistics.
Significantly improved the germination rate of *Carex glabra* rhizomes. IBA, NAA, and 6-BA at a concentration of 60 mg/L and a soaking time of 15 min increased the germination rate by 66.3% to 136.3%, and different hormone combinations increased the germination rate by 136.3% to 231.9%, thus solving the technical bottleneck in seedling propagation.
Smart Images
Figure CN121569748A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of lawn and ground cover plant propagation technology, and in particular to a method for improving the germination rate of white sedge rhizomes. Background Technology
[0002] White-winged sedge (Carex rigescens), also known as small goat's beard grass, is a subspecies of the genus Carex in the family Cyperaceae. It is an important native grass species in my country, possessing excellent traits such as early greening, long green period, tolerance to trampling, low maintenance, and high ornamental value in northern my country. Furthermore, its slender leaves and attractive appearance provide good coverage after establishment, and its tolerance to trampling and relatively low management make it suitable for use as a lawn grass in open forests and recreational lawns in northern my country. However, the seeds of white-winged sedge have a certain degree of dormancy, resulting in slow germination, which limits its large-scale application in landscaping.
[0003] In actual production, after sowing, a long waiting period is required for the grass to turn green. During this period, it is susceptible to weed invasion, drought stress, and soil compaction, resulting in a high risk of turf establishment failure and high reseeding costs. This has become a technical bottleneck in landscaping projects and ecological restoration, making it a "want to use but dare not use" approach. Even more challenging is that traditional pre-sowing treatments such as cold water soaking, short-term gibberellin treatment, or mechanical bark breaking have limited effectiveness in breaking the dormancy of *Carex glabra*, and are often crude in operation, with vague parameters and a lack of replicable quantitative standards. Furthermore, in order to save time, production often blindly increases the sowing rate, resulting in high-density seedlings that, due to slow early growth and weak competitiveness, still fall into a vicious cycle of "not germinating, not growing fast, and not establishing a turf." This wastes precious native seed resources, delays landscaping projects, and doubles maintenance costs. Ultimately, this causes designers and construction teams to lose confidence in native grass species, opting instead for imported cool-season turfgrasses that consume more water and require more maintenance, further exacerbating the dual pressures of water conservation and ecological adaptability in northern urban green spaces.
[0004] Therefore, developing a quantitative, efficient, and stable method to improve the germination rate of white sedge rhizomes is of great significance for promoting the application of native grass species and alleviating the ecological pressure on green spaces in northern cities. Summary of the Invention
[0005] The purpose of this invention is to provide a method for improving the germination rate of white sedge rhizomes. This invention quantitatively analyzes the germination rate of white sedge seedlings under different concentrations, different soaking times and different hormones, aiming to break through the technical bottleneck in seedling propagation, provide key support for establishing an efficient and stable white sedge propagation technology system, and help improve the rapid establishment of turfgrass.
[0006] To achieve the above objectives, the present invention adopts the following technical solution:
[0007] This invention provides a method for improving the germination rate of *Carex glabra* rhizomes, comprising the following steps:
[0008] Soak the white sedge seedlings in a hormone solution;
[0009] Place the soaked white sedge seedlings flat on the seedbed;
[0010] The hormones mentioned include at least one of IBA, NAA, and 6-BA.
[0011] Preferably, if the hormone includes IBA, the concentration of IBA is 20-80 mg / L;
[0012] If the hormone includes NAA, then the concentration of NAA is 20~80 mg / L;
[0013] If the hormone includes 6-BA, the concentration of 6-BA is 20~80 mg / L.
[0014] The concentration of the hormone solution is 20~80 mg / L.
[0015] Preferably, in the step of soaking the white sedge seedlings in the hormone solution, the soaking time is 15-60 minutes.
[0016] Preferably, the hormone is any one of IBA, NAA, and 6-BA, and the concentration of the hormone solution is 60 mg / L.
[0017] Preferably, the hormone comprises IBA and NAA, and the concentration of both IBA and NAA in the hormone solution is 60 mg / L;
[0018] Alternatively, the hormone comprises IBA and 6-BA, wherein the concentration of both IBA and 6-BA in the hormone solution is 60 mg / L;
[0019] Alternatively, the hormone comprises 6-BA and NAA, and the concentration of both 6-BA and NAA in the hormone solution is 60 mg / L;
[0020] Alternatively, the hormones include IBA, NAA, and 6-BA, and the concentrations of IBA, NAA, and 6-BA in the hormone solution are all 60 mg / L.
[0021] Preferably, the soaking time is 15 minutes.
[0022] Preferably, the white sedge is taken from its growing place, the root soil is cleaned, single plants with intact buds are selected, and the stems and leaves above the rhizome are trimmed to 2cm and the roots below the rhizome to 2cm, thus obtaining white sedge seedlings.
[0023] The method of the present invention for improving the germination rate of *Carex glabra* rhizomes has the following advantages over the prior art:
[0024] The method for improving the germination rate of *Carex glabra* rhizomes of this invention involves soaking *Carex glabra* seedlings in a hormone solution, which can enhance the germination rate of *Carex glabra* rhizomes. NAA, 6-BA, and IBA all showed the highest rhizome germination rate at a concentration of 60 mg / L, significantly higher than other concentrations, with germination rates of 22.2%, 24.8%, and 31.5%, respectively, representing increases of 66.3%, 86.3%, and 136.3% compared to the 0 mg / L treatment. NAA, 6-BA, and IBA all showed the highest rhizome germination rate at a soaking time of 15 min, significantly higher than other soaking times, with germination rates of 22.2%, 24.8%, and 31.5%, respectively, representing increases of 66.3%, 86.3%, and 136.3% compared to the 0 min soaking treatment. At day 12, the germination rate of *Carex glabra* rhizomes treated with IBA+6-BA was 64.7%, significantly higher than other hormone treatments (p<0.05). Compared with the control (i.e., no hormone soaking), the germination rates of *Carex glabra* rhizomes treated with IBA, NAA, 6-BA, IBA+NAA, IBA+6-BA, 6-BA+NAA, and IBA+NAA+6-BA increased by 136.3%, 66.3%, 86.2%, 152.5%, 231.9%, 198.1%, and 151.9%, respectively. In conclusion, to improve the germination rate of *Carex glabra* seedlings, the seedlings should be soaked in 60 mg / L IBA+6-BA for 15 min. Attached Figure Description
[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0026] Figure 1 The effect of different concentrations of hormone NAA on the germination rate of *Carex glabra* rhizomes in Example 1;
[0027] Figure 2 The effect of different concentrations of hormone 6-BA on the germination rate of *Carex glabra* rhizomes in Example 1;
[0028] Figure 3 The effect of different concentrations of hormone IBA on the germination rate of *Carex glabra* rhizomes in Example 1;
[0029] Figure 4 The effect of different soaking times of hormone NAA on the germination rate of *Carex glabra* rhizomes in Example 2;
[0030] Figure 5 The effect of hormone 6-BA at different soaking times on the germination rate of *Carex glabra* rhizomes in Example 2;
[0031] Figure 6 The effect of different soaking times of hormone IBA on the germination rate of *Carex glabra* rhizomes in Example 2;
[0032] Figure 7 The effect of different hormones on the germination rate of *Carex glabra* rhizomes in Example 3. Detailed Implementation
[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.
[0034] It should be noted that the order of description of the following embodiments is not intended to limit the preferred order of embodiments. Furthermore, in the description of this application, the term "comprising" means "including but not limited to". Various embodiments of the present invention may exist in the form of a range; it should be understood that the description in the form of a range is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of the invention; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single numerical values within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single digits within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. Additionally, whenever a numerical range is indicated herein, it means including any referenced number (fraction or integer) within the indicated range.
[0035] This application provides a method for improving the germination rate of *Carex glabra* rhizomes, including the following steps:
[0036] S1. Soak the white sedge seedlings in a hormone solution;
[0037] S2. Place the soaked white sedge seedlings flat on the seedbed;
[0038] Among them, hormones include at least one of IBA, NAA, and 6-BA.
[0039] Specifically, NAA stands for α-naphthaleneacetic acid, with the chemical formula C. 12 H 10O2 promotes cell division and elongation, breaks seed dormancy, accelerates rooting and germination, and improves seedling survival rate; 6-BA, or 6-benzylaminopurine, has the chemical formula C. 12 H 11 N5 promotes cell division, delays aging, enhances seedling resistance, and improves germination uniformity; IBA, or indolebutyric acid, has the chemical formula C5. 12 H 13 NO2 has the effect of strongly promoting root growth, breaking dormancy, and improving the root vitality and germination rate of seedlings; the present invention soaks the seedlings of *Carex glabra* in a hormone solution, which can improve the germination rate of *Carex glabra* rhizomes.
[0040] In some embodiments, if the hormone includes IBA, the concentration of IBA is 20-80 mg / L;
[0041] If the hormone includes NAA, then the concentration of NAA is 20~80 mg / L;
[0042] If the hormone includes 6-BA, then the concentration of 6-BA is 20~80 mg / L.
[0043] Specifically, the hormone is added to distilled water to obtain a hormone solution; 20~80 mg / L means that the weight of hormone added per liter of distilled water is 20~80 mg. If the hormone is IBA, and the IBA concentration is 20~80 mg / L, it means that the weight of IBA added per liter of distilled water is 20~80 mg; specifically, the hormone concentrations are 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L.
[0044] In some embodiments, the soaking time in the step of soaking the white sedge seedlings in the hormone solution is 15 to 60 minutes, such as 15 minutes, 30 minutes, 45 minutes, or 60 minutes.
[0045] In some embodiments, the hormone is any one of IBA, NAA, and 6-BA, and the concentration of the hormone solution is 60 mg / L.
[0046] In some embodiments, the hormones include IBA and NAA, wherein the concentration of IBA is 60 mg / L and the concentration of NAA is 60 mg / L.
[0047] In some embodiments, the hormones include IBA and 6-BA, wherein the concentration of IBA is 60 mg / L and the concentration of 6-BA is 60 mg / L.
[0048] In some embodiments, the hormones include 6-BA and NAA, wherein the concentration of 6-BA is 60 mg / L and the concentration of NAA is 60 mg / L.
[0049] In some embodiments, the hormones include IBA, NAA, and 6-BA, wherein the concentrations of IBA, NAA, and 6-BA are 60 mg / L, respectively.
[0050] In some embodiments, the soaking time is 15 minutes.
[0051] In some embodiments, the white sedge is removed from its growing site, the soil around its roots is cleaned, single plants with intact buds are selected, and the stems and leaves above the rhizome are pruned to 2cm and the roots below the rhizome to 2cm, thus obtaining white sedge seedlings.
[0052] In some embodiments, the white sedge was removed from the experimental site using a sod cutter, the root soil was cleaned, and individual plants with intact buds were pruned, leaving 2 cm of stems and leaves on the rhizome and 2 cm of the lower root system. After completing the above operations, the seedlings with intact buds were transferred to soak in hormones of different concentrations, and the effects of different hormones and hormone combinations on the germination rate of the white sedge rhizomes were investigated.
[0053] After soaking, the white sedge seedlings were laid flat on the seedbed, and the germination rate was counted every 3 days.
[0054] The method for improving the germination rate of *Carex glabra* rhizomes of this invention shows that NAA, 6-BA, and IBA all exhibited the highest germination rates at a concentration of 60 mg / L, significantly higher than other concentrations, with germination rates of 22.2%, 24.8%, and 31.5%, respectively. These rates represent increases of 66.3%, 86.25%, and 136.3% compared to the 0 mg / L treatment. Furthermore, the highest germination rates were observed with a soaking time of 15 min, significantly higher than other soaking times, with germination rates of 22.2%, 24.8%, and 31.5%, respectively. These rates represent increases of 66.3%, 86.3%, and 136.3% compared to the 0 min soaking treatment. Under different stimuli, on day 12, the germination rate of *Carex glabra* rhizomes treated with IBA+6-BA was 64.7%, significantly higher (p<0.05) than other hormone treatments. Compared with the control (i.e., no hormone soaking), the germination rates of *Carex glabra* rhizomes under IBA, NAA, 6-BA, IBA+NAA, IBA+6-BA, 6-BA+NAA, and IBA+NAA+6-BA treatments increased by 136.3%, 66.3%, 86.2%, 152.5%, 231.9%, 198.1%, and 151.9%, respectively. In conclusion, to improve the germination rate of *Carex glabra* seedlings, the seedlings should be soaked in 60 mg / L IBA+6-BA for 15 min.
[0055] The following specific embodiments further illustrate the method for improving the germination rate of *Carex glabra* rhizomes according to this application. This section further illustrates the content of the present invention in conjunction with specific embodiments, but should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in the art.
[0056] Example 1
[0057] This application provides a method for improving the germination rate of *Carex glabra* rhizomes, including the following steps:
[0058] S1. Take the white sedge out of its growing place, clean the soil around the roots, select individual plants with intact buds, and prune them until the stems and leaves above the rootstock are 2cm and the roots below are 2cm. This will give you white sedge seedlings.
[0059] S2. Soak the white sedge seedlings in a hormone solution; the concentration of the hormone solution is 0~80 mg / L, and the hormone is NAA; the soaking time is 15 min.
[0060] S3. Place the soaked white sedge seedlings flat on the seedbed and count the germination rate.
[0061] Figure 1 The effect of different concentrations of hormone NAA on the germination rate of *Carex glabra* rhizomes in Example 1.
[0062] Furthermore, following the method in Example 1, the hormone NAA was replaced with 6-BA, and everything else remained the same as in Example 1. Figure 2 The effect of different concentrations of hormone 6-BA on the germination rate of rhizomes of *Carex glabra*.
[0063] Following the method in Example 1, the hormone NAA was replaced with IBA, and everything else was the same as in Example 1. Figure 3 The effect of different concentrations of the hormone IBA on the germination rate of the rhizomes of *Carex glabra*.
[0064] Figures 1-3 In the figure, 0 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L represent the hormone concentrations used when soaking for 15 minutes, and 3d, 6d, 9d, and 12d represent the time intervals at which the rhizome germination rate of *Carex glabra* was calculated after soaking in different concentrations of hormone. The lowercase letters at the top and bottom of the figure indicate significant differences (P<0.05) at different nitrogen fertilizer levels within the same density; *, **, and *** indicate significant differences at P<0.05, P<0.01, and P<0.001 levels, respectively.
[0065] Depend on Figures 1-3It was found that under the treatment of three hormones at different concentrations, the germination rate of *Carex glabra* gradually increased with the number of days. For NAA hormone, on day 3, the germination rate of *Carex glabra* rhizomes treated with 60 mg / L was significantly higher than that treated with 20 mg / L, 40 mg / L, and 80 mg / L, increasing by 125.0%, 157.1%, and 157.1%, respectively. On day 6, the germination rate of *Carex glabra* rhizomes treated with 60 mg / L was significantly higher than other concentrations, increasing by 60.0%–166.7%. On day 12, the germination rate of *Carex glabra* rhizomes treated with 60 mg / L NAA was 40.7%, significantly higher than the concentrations at other time points. Compared with the control (i.e., 0 mg / L NAA, soaked in distilled water), the germination rate of *Carex glabra* rhizomes increased by 9.4%, 66.3%, and 43.1% under NAA treatments of 40 mg / L, 60 mg / L, and 80 mg / L, respectively. Figure 1 At day 3, the germination rate of *Carex glabra* rhizomes treated with 80 mg / L and 0 mg / L was not significantly different (p>0.05). The germination rates of *Carex glabra* rhizomes treated with 20 mg / L, 40 mg / L, and 60 mg / L were not significantly different (p>0.05), but were significantly higher than 0 mg / L, increasing by 136.4%, 136.4%, and 118.2%, respectively. At day 6, the germination rates of *Carex glabra* rhizomes treated with 20 mg / L, 40 mg / L, and 80 mg / L were not significantly different from 0 mg / L (p>0.05), but the germination rates of 60 mg / L treated with 60 mg / L were significantly higher than 0 mg / L, increasing by 136.4%, 136.4%, and 118.2%, respectively. The germination rate of *Carex glabra* rhizomes treated with 60 mg / L was significantly higher (p<0.05) than that treated with 0 mg / L, increasing by 113.3%. The germination rate of *Carex glabra* rhizomes at day 9 and day 6 showed a consistent trend, with the germination rate at 60 mg / L significantly higher (p<0.05) than that at 0 mg / L, increasing by 74.5%. At day 12, there was no significant difference in the germination rate of *Carex glabra* rhizomes between the 40 mg / L treatment and the other treatments (p>0.05), but the germination rate at 60 mg / L was significantly higher (p<0.05) than that at 0 mg / L, 20 mg / L, and 80 mg / L, increasing by 77.8%, 64.1%, and 96.9%, respectively. On day 12, the germination rate of *Carex glabra* rhizomes treated with 60 mg / L 6-BA was 42.7%, significantly higher than that of other concentrations at different time points. Compared with the control (0 mg / L 6-BA, soaked in distilled water), the germination rate of *Carex glabra* rhizomes increased by 35.0%, 37.5%, 86.3%, and 4.4% respectively under 6-BA treatments of 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L. Figure 2Regarding IBA hormone, on day 3, there was no significant difference in the germination rate of *Carex glabra* rhizomes under different IBA concentrations (p>0.05); on day 6, there was no significant difference in the germination rate of *Carex glabra* rhizomes between the 20 mg / L, 40 mg / L, and 80 mg / L treatments and the 0 mg / L treatment (p>0.05), but the germination rate under the 60 mg / L treatment was significantly higher (p<0.05) than that under the 0 mg / L and 20 mg / L treatments, increasing by 113.3% and 100.0%, respectively; on day 9, there was no significant difference in the germination rate of *Carex glabra* rhizomes between the 20 mg / L, 40 mg / L, and 80 mg / L treatments and the 0 mg / L treatment (p>0.05), but the germination rate under the 60 mg / L treatment was significantly higher (p<0.05) than that under the 0 mg / L and 80 mg / L treatments, increasing by 172.3% and 93.9%, respectively. On day 12, there was no significant difference in the germination rate of *Carex glabra* rhizomes between the 80 mg / L and 0 mg / L treatments (p>0.05). However, the germination rates at 20 mg / L, 40 mg / L, and 60 mg / L treatments were significantly higher (p<0.05) than at 0 mg / L, increasing by 80.6%, 86.1%, and 105.6%, respectively. On day 12, the germination rate of *Carex glabra* rhizomes treated with 60 mg / L IBA was 49.3%, significantly higher (p<0.05) than at other concentrations. Compared to the control (0 mg / L IBA, soaked in distilled water), the germination rate of *Carex glabra* rhizomes increased by 61.3%, 69.4%, 136.3%, and 42.5% at 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L IBA treatments, respectively. Figure 3 ).
[0066] Example 2
[0067] This application provides a method for improving the germination rate of *Carex glabra* rhizomes, including the following steps:
[0068] S1. Take the white sedge out of its growing place, clean the soil around the roots, select individual plants with intact buds, and prune them until the stems and leaves above the rootstock are 2cm and the roots below are 2cm. This will give you white sedge seedlings.
[0069] S2. Soak the white sedge seedlings in a hormone solution; the hormone solution concentration is 60 mg / L, the hormone is NAA; the soaking time is 0~60 min;
[0070] S3. Place the soaked white sedge seedlings flat on the seedbed and count the germination rate.
[0071] Figure 4 The effect of different soaking times of hormone NAA on the germination rate of *Carex glabra* rhizomes in Example 2.
[0072] Furthermore, following the method in Example 2, the hormone NAA was replaced with 6-BA, and everything else remained the same as in Example 2. Figure 5 The effect of different soaking times of hormone 6-BA on the germination rate of *Carex glabra* rhizomes was investigated. Following the method in Example 2, hormone NAA was replaced with IBA, and all other aspects remained the same as in Example 2. Figure 6 The effect of different soaking times of the hormone IBA on the germination rate of the rhizomes of *Carex glabra*.
[0073] Figures 4-6 In the figure, 0 min, 15 min, 30 min, 45 min, and 60 min represent the soaking time of *Carex glabra* in a hormone solution of 60 mg / L, and 3 d, 6 d, 9 d, and 12 d represent the time for calculating the germination rate of *Carex glabra* after different soaking times. The lowercase letters at the top and bottom of the figure indicate significant differences (P < 0.05) at different nitrogen fertilizer levels at the same density; *, **, and *** indicate significant differences at the P < 0.05, P < 0.01, and P < 0.001 levels, respectively.
[0074] Depend on Figures 4-6It was found that the germination rate of *Carex glabra* gradually increased with the number of days under the treatment of three hormones and different soaking times. For NAA hormone, on day 3, there was no significant difference in the germination rate of *Carex glabra* rhizomes treated with different soaking times (p>0.05); on day 6, there was no significant difference in the germination rate of *Carex glabra* rhizomes treated with 15 min and 60 min soaking times, but the germination rate was significantly higher (p<0.05) than that treated with 0 min, 30 min, and 45 min, increasing by 60.0%, 54.8%, and 41.2%, respectively; there was no significant difference in the germination rate of *Carex glabra* rhizomes treated with 0 min, 30 min, 45 min, and 60 min soaking times (p>0.05). On day 9, the germination rate of *Carex glabra* rhizomes soaked for 15 min was not significantly different from that of the 30 min and 45 min treatments (p>0.05), but was significantly higher (p<0.05) than that of 0 min and 60 min, increasing by 66.0% and 39.3%, respectively. The germination rate of *Carex glabra* rhizomes soaked for 30 min was not significantly different from that of the 15 min, 30 min, 45 min and 60 min treatments (p>0.05), but was significantly higher (p<0.05) than that of 0 min, increasing by 53.2%. The germination rate of *Carex glabra* rhizomes soaked for 0 min, 45 min and 60 min treatments was not significantly different (p>0.05). On day 12, the germination rate of *Carex glabra* rhizomes treated with soaking for 15 min and 30 min showed no significant difference (p>0.05) compared to those treated with 45 min and 60 min, but was significantly higher (p<0.05) than that treated with 0 min, increasing by 69.4% and 51.4% respectively. There were no significant differences in germination rates among the 0 min, 45 min, and 60 min soaking treatments. On day 12, the germination rate of *Carex glabra* rhizomes soaked with NAA for 15 min was 40.7%, significantly higher (p<0.05) than other soaking times. Compared to the control (i.e., 0 mg / L NAA, soaked in distilled water), the germination rates of *Carex glabra* rhizomes treated with NAA for 15 min, 30 min, 45 min, and 60 min increased by 66.3%, 42.5%, 27.5%, and 28.1% respectively. Figure 4). 6-BA hormone, on day 3, the germination rate of *Carex glabra* rhizomes treated with 15 min, 30 min, and 45 min soaking was not significantly different from that treated with 60 min (p>0.05), but significantly higher (p<0.05) than that treated with 0 min, increasing by 118.2%, 100.0%, and 109.1%, respectively; there was no significant difference in germination rate between the 0 min and 60 min soaking treatments (p>0.05). On day 6, the germination rate of *Carex glabra* rhizomes soaked for 15 min was not significantly different from that of *Carex glabra* rhizomes soaked for 30 min, 45 min, and 60 min (p>0.05), but was significantly higher (p<0.05) than that of 0 min, increasing by 113.3%. The germination rate of *Carex glabra* rhizomes soaked for 0 min was not significantly different from that of *Carex glabra* rhizomes soaked for 30 min, 45 min, and 60 min (p>0.05). On day 9, the differences in germination rate of *Carex glabra* rhizomes under different soaking time treatments were consistent with those on day 6. The germination rate of *Carex glabra* rhizomes soaked for 15 min increased by 74.5% compared to 0 min. On day 12, the germination rate of *Carex glabra* rhizomes soaked for 15 min showed no significant difference (p>0.05) compared to the germination rates of 45 min and 60 min treatments, but was significantly (p<0.05) higher than that of 0 min and 30 min, increasing by 77.8% and 40.7%, respectively. The germination rate of *Carex glabra* rhizomes soaked for 45 min and 60 min showed no significant difference (p>0.05) compared to the germination rates of 15 min and 30 min treatments, but was significantly (p<0.05) higher than that of 0 min, increasing by 61.1% and 52.8%, respectively. On day 12, the germination rate of *Carex glabra* rhizomes soaked in 6-BA for 15 min was 42.7%, significantly higher than that of other soaking times. Compared with the control (i.e., 0 mg / L 6-BA, soaked in distilled water), the germination rate of *Carex glabra* rhizomes treated with 6-BA for 15 min, 30 min, 45 min, and 60 min increased by 86.3%, 34.3%, 67.5%, and 56.9%, respectively. Figure 5With IBA hormone, on day 3, the germination rate of *Carex glabra* rhizomes treated with 15 min and 30 min showed no significant difference (p>0.05), but was significantly (p<0.05) higher than that treated with 0 min, 45 min, and 60 min, increasing by 245.5%, 58.3%, and 137.5%, respectively. The germination rate of *Carex glabra* rhizomes treated with 30 min showed no significant difference (p>0.05) compared to those treated with 15 min and 45 min, but was significantly (p<0.05) higher than that treated with 0 min and 60 min, increasing by 172.7% and 87.5%, respectively. There was no significant difference in germination rate between 0 min and 60 min treatments. On day 6, the germination rate of *Carex glabra* rhizomes soaked for 15 min showed no significant difference (p>0.05) compared to the germination rates of *Carex glabra* rhizomes soaked for 30 min, 45 min, and 60 min, but was significantly higher (p<0.05) than that of 0 min, increasing by 113.3%. There were no significant differences in germination rates among the 0 min, 30 min, 45 min, and 60 min soaking treatments. On day 9, the germination rate of *Carex glabra* rhizomes soaked for 15 min with IBA was significantly higher (p<0.05) than other soaking times, increasing by 68.4-172.3%. The germination rates of *Carex glabra* rhizomes soaked for 30 min and 45 min showed no significant difference (p>0.05) compared to 60 min, but were significantly higher (p<0.05) than those of 0 min, increasing by 61.7% and 57.4%, respectively. There was no significant difference in germination rates between the 0 min and 60 min soaking treatments. On day 12, the germination rate of *Carex glabra* rhizomes treated with 15 min of soaking showed no significant difference (p>0.05) compared to those treated with 30 min and 45 min, but was significantly (p<0.05) higher than that treated with 0 min and 60 min, increasing by 105.6% and 87.3%, respectively. There were no significant differences (p>0.05) in the germination rates of *Carex glabra* rhizomes treated with 0 min, 30 min, 45 min, and 60 min of soaking. On day 12, the germination rate of *Carex glabra* rhizomes soaked with IBA for 15 min was 49.3%, significantly higher than that of other soaking times. Compared to the control (i.e., 0 mg / L IBA, soaked in distilled water), the germination rates of *Carex glabra* rhizomes treated with IBA for 15 min, 30 min, 45 min, and 60 min increased by 136.3%, 61.3%, 67.5%, and 22.5%, respectively. Figure 6 ).
[0075] Example 3
[0076] This application provides a method for improving the germination rate of *Carex glabra* rhizomes, including the following steps:
[0077] S1. Take the white sedge out of its growing place, clean the soil around the roots, select individual plants with intact buds, and prune them until the stems and leaves above the rootstock are 2cm and the roots below are 2cm. This will give you white sedge seedlings.
[0078] S2. Soak the white sedge seedlings in different hormone solutions for 15 minutes.
[0079] Among them, hormones include IBA and NAA (corresponding to Figure 7 (IBA + NAA), with IBA concentration of 60 mg / L and NAA concentration of 60 mg / L;
[0080] Alternatively, hormones include IBA and 6-BA (corresponding to...) Figure 7 The concentration of IBA was 60 mg / L, and the concentration of 6-BA was 60 mg / L.
[0081] Alternatively, hormones include 6-BA and NAA (corresponding to...) Figure 7 The concentration of 6-BA was 60 mg / L and the concentration of NAA was 60 mg / L.
[0082] Or, hormones include IBA, NAA, and 6-BA (corresponding to...) Figure 7 The concentrations of IBA, NAA, and 6-BA were 60 mg / L, 60 mg / L, and 6-BA, respectively.
[0083] Or, the hormone is IBA (corresponding to...) Figure 7 The concentration of IBA was 60 mg / L.
[0084] Or, the hormone is NAA (corresponding to...) Figure 7 The concentration of NAA was 60 mg / L.
[0085] Or, the hormone is 6-BA (corresponding to...) Figure 7 The concentration of 6-BA was 60 mg / L.
[0086] Meanwhile, the hormone solution was replaced with distilled water as a control (corresponding to...). Figure 7 (China CK).
[0087] The effects of different hormones on the germination rate of *Carex glabra* rhizomes in Example 7 are as follows: Figure 7 As shown.
[0088] Figure 7To illustrate the effect of different hormones on the germination rate of *Carex glabra* rhizomes, 3d, 6d, 9d, and 12d represent the time when the germination rate of *Carex glabra* was calculated after soaking in hormones for 15 minutes. The lowercase letters above the bars in the figure indicate that (P<0.05) the germination rate of *Carex glabra* rhizomes under different hormone treatments reached a significant difference on the same day.
[0089] Depend on Figure 7It can be seen that under different hormone treatments, the germination rate of *Carex glabra* gradually increases with the number of days. On day 3, the germination rate of *Carex glabra* rhizomes treated with IBA+6-BA was not significantly different from that treated with 6-BA+NAA (p>0.05), but significantly higher (p<0.05) than that treated with CK, IBA, NAA, 6-BA, IBA+NAA, and IBA+NAA+6-BA, increasing by 600.0%, 102.6%, 327.8%, 220.8%, 156.7%, and 48.1%, respectively. The germination rate of *Carex glabra* rhizomes treated with 6-BA+NAA was not significantly different from that treated with IBA+NAA and IBA+NAA+6-BA (p>0.05), but significantly higher (p<0.05) than that treated with CK, IBA, NAA, 6-BA, and IBA+NAA, increasing by 518.2%, 78.9%, 277.8%, and 183.3%, respectively. The germination rate of *Carex glabra* rhizomes treated with IBA+NAA+6-BA was not significantly different from that treated with IBA, IBA+NAA, and 6-BA+NAA (p>0.05), but was significantly higher (p<0.05) than CK, NAA, and 6-BA, increasing by 372.7%, 188.9%, and 116.7%, respectively. The germination rate of *Carex glabra* rhizomes treated with IBA was not significantly different from that treated with NAA, 6-BA, and IBA+NAA, but was significantly higher (p<0.05) than CK, increasing by 245.5%. On day 6, the germination rate of *Carex glabra* rhizomes under the IBA+6-BA, 6-BA+NAA treatments was not significantly different from that under the IBA, 6-BA, IBA+NAA, and IBA+NAA+6-BA treatments, but was significantly higher than that under the control (CK) and NAA treatments, increasing by 193.3%, 83.3%, 203.3%, and 89.6%, respectively. The germination rate of *Carex glabra* rhizomes under the IBA, 6-BA, IBA+NAA, and IBA+NAA+6-BA treatments was not significantly different from that under the NAA treatment (p>0.05), but was significantly higher than that under the control (CK), increasing by 113.3%, 113.3%, 120.0%, and 113.3%, respectively.On day 9, there was no significant difference in the rhizome germination rate of *Carex glabra* under the IBA+6-BA and 6-BA+NAA treatments, but the rates were significantly higher than those under the control (CK), IBA, NAA, 6-BA, IBA+NAA, and IBA+NAA+6-BA treatments, increasing by 266.0%, 34.4%, 120.5%, 109.8%, 19.4%, and 30.3%, respectively. There was no significant difference in the rhizome germination rate of *Carex glabra* under the four hormone treatments (IBA, IBA+NAA, 6-BA+NAA, and IBA+NAA+6-BA), but all were significantly higher than those under the control (CK), NAA, and 6-BA treatments, increasing by 64.1%, 56.1%, 84.6%, 75.6%, 93.6%, 84.1%, and 69.2%, respectively. The germination rate of *Carex glabra* rhizomes under IBA+6-BA treatment was 61.0% and 61.0%, respectively. There was no significant difference in germination rate between the NAA and 6-BA treatments, but the rates were significantly higher than those under the control (CK), increasing by 66.0% and 74.5%, respectively. The germination rate of *Carex glabra* rhizomes under hormone treatment was significantly higher than that under the control (CK), increasing by 74.5% to 266.0%. At day 12, the germination rate of *Carex glabra* rhizomes under IBA+6-BA treatment was not significantly different from that under IBA+NAA, 6-BA+NAA, and IBA+NAA+6-BA treatments, but significantly higher than that under the control (CK), IBA, NAA, and 6-BA treatments, increasing by 169.4%, 31.1%, 59.0%, and 51.6%, respectively. The germination rate of *Carex glabra* rhizomes under IBA+NAA and 6-BA+NAA treatments was significantly higher than that under the control (CK), IBA, NAA, and 6-BA treatments, increasing by 169.4%, 31.1%, 59.0%, and 51.6%, respectively. The germination rate of *Carex glabra* rhizomes under NAA+6-BA treatment was not significantly different from that under IBA, but significantly higher than that under NAA, increasing by 34.4% and 36.9%, respectively. The germination rate of *Carex glabra* rhizomes under NAA treatment was not significantly different from that under IBA, 6-BA, and IBA+NAA+6-BA treatments, but significantly higher than that under control (CK), increasing by 64.9%. The germination rate of *Carex glabra* rhizomes under hormone treatment was significantly higher than that under control (CK) (p<0.05), increasing by 64.9-169.4%. On day 12, the germination rate of *Carex glabra* rhizomes under the IBA+6-BA treatment was 64.7%, significantly higher than that under other hormone treatments (p<0.05). Compared with the control (i.e., no hormone soaking), the germination rates of *Carex glabra* rhizomes under the IBA, NAA, 6-BA, IBA+NAA, IBA+6-BA, 6-BA+NAA, and IBA+NAA+6-BA treatments increased by 136.3%, 66.3%, 86.2%, 152.5%, 231.9%, 198.1%, and 151.9%, respectively.
[0090] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for improving the germination rate of *Carex glabra* rhizomes, characterized in that, Includes the following steps: Soak the white sedge seedlings in a hormone solution; Place the soaked white sedge seedlings flat on the seedbed; The hormones mentioned include at least one of IBA, NAA, and 6-BA.
2. The method for improving the germination rate of *Carex glabra* rhizomes as described in claim 1, characterized in that, If the hormone includes IBA, the concentration of IBA is 20-80 mg / L; If the hormone includes NAA, then the concentration of NAA is 20~80 mg / L; If the hormone includes 6-BA, the concentration of 6-BA is 20~80 mg / L.
3. The method for improving the germination rate of *Carex glabra* rhizomes as described in claim 1, characterized in that, In the step of soaking the white sedge seedlings in the hormone solution, the soaking time is 15-60 minutes.
4. The method for improving the germination rate of *Carex glabra* rhizomes as described in claim 2, characterized in that, The hormone is any one of IBA, NAA, and 6-BA, and the concentration of the hormone solution is 60 mg / L.
5. The method for improving the germination rate of *Carex glabra* rhizomes as described in claim 1, characterized in that, The hormones include IBA and NAA, and the concentration of both IBA and NAA in the hormone solution is 60 mg / L; Alternatively, the hormone comprises IBA and 6-BA, wherein the concentration of both IBA and 6-BA in the hormone solution is 60 mg / L; Alternatively, the hormone comprises 6-BA and NAA, and the concentration of both 6-BA and NAA in the hormone solution is 60 mg / L; Alternatively, the hormones include IBA, NAA, and 6-BA, and the concentrations of IBA, NAA, and 6-BA in the hormone solution are all 60 mg / L.
6. The method for improving the germination rate of *Carex glabra* rhizomes as described in claim 3, characterized in that, Soaking time is 15 minutes.
7. The method for improving the germination rate of *Carex glabra* rhizomes as described in claim 1, characterized in that, Remove the white sedge from its growing area, clean the soil around the roots, select individual plants with intact buds, and prune them until the stems and leaves above the rhizome are 2cm long and the roots below are 2cm long, thus obtaining white sedge seedlings.