A method for dispensing liquid by using a pipette with wide-range automatic and precise control

Abstract

The application discloses a large-range automatic precise control liquid dispensing method of a pipette, and belongs to the technical field of the pipette. In the application, a single device is combined with a double peristaltic pump mode, so that the wide-range liquid dispensing demand is covered, a plurality of pipettes are not needed to be matched, cost and space are saved, integer dispensing plus a high-precision iteration of a residual amount is adopted, the minimum precision reaches 0.01 muL, non-integer volume demand is accurately adapted, three modes of automatic, high-precision and manual are provided, different use scenes are adapted, and the operation threshold is reduced, manual contact is reduced, liquid pollution and volatilization risk are reduced, and the application is suitable for precise liquid dispensing in multiple fields.

Description

Technical Field

[0001] This invention relates to the field of pipette technology, and more specifically to a pipette dispensing method with wide-range automatic and precise control. Background Technology

[0002] As a core tool in fields such as chemical analysis, the accuracy and efficiency of pipette dispensing directly affect the reliability of experimental results, the accuracy of detection data, and the stability of production processes. In practical applications, pipette dispensing needs to meet the requirements for liquid transfer of different volumes (from microliters to milliliters).

[0003] Existing pipette solutions typically require multiple pipettes with different capacities to work together, necessitating multiple dispensing operations. These methods are mostly manual, making them time-consuming, labor-intensive, and inefficient. Therefore, this paper proposes a pipette solution preparation method with wide-range, automated, and precise control. Summary of the Invention

[0004] The technical problem to be solved by this invention is: how to solve the problem that existing pipettes generally require multiple pipettes with different capacities to work together, require multiple liquid collections, are mostly in manual mode, are time-consuming and labor-intensive, and have low work efficiency when preparing liquids, and provide a pipette preparation method with large-scale automatic and precise control.

[0005] The present invention solves the above-mentioned technical problems through the following technical solution, and the present invention includes the following steps:

[0006] S1: Obtain the volume of the liquid to be prepared as the target value;

[0007] S2: Select the working mode, which includes automatic mode, high precision mode and manual mode;

[0008] S3: When the working mode is set to automatic mode, the target value is rounded down multiple times to obtain the corresponding rounded value. Based on each rounded value and according to the liquid volume of each peristaltic pump, the rotation volume of each pump is allocated until the remaining amount of the target value is zero, and the liquid extraction is completed.

[0009] S4: When the working mode is selected as high precision mode, the minimum precision is directly calculated based on the target value. The rotation amount of each pump is allocated according to the minimum precision quantity and the liquid volume of each peristaltic pump per revolution. Liquid collection is completed after the rotation ends.

[0010] S5: When the working mode is selected as manual mode, it is used to directly input the number of rotations and direction of the two peristaltic pumps. The two peristaltic pumps will rotate according to the input number of rotations and direction, and the liquid collection will be completed after the rotation is finished.

[0011] Furthermore, the pipette includes two peristaltic pumps and an air chamber. The two peristaltic pumps are connected to the air chamber via a hose. An inlet is located below the air chamber. The two peristaltic pumps are controlled by motors to rotate. A pipette tip is installed on the inlet.

[0012] Furthermore, in step S1, the target value is obtained by direct manual input or voice input.

[0013] Furthermore, in step S3, the specific processing procedure is as follows:

[0014] S31: First, round the target value down. Then, based on the rounded integer value, distribute the rotation of each pump according to the liquid volume of each peristaltic pump.

[0015] S32: Continue to round down the remaining value of the target value to the tenths place. Based on the tenths place value, continue to repeatedly distribute the rotation amount of each pump according to the single-turn liquid volume of the two peristaltic pumps.

[0016] S33: Continue to round down the remaining value of the target value to obtain the percentile value. Based on the percentile value, continue to repeatedly distribute the rotation amount of each pump according to the single-turn liquid volume of the two peristaltic pumps.

[0017] S34: Continue until the remaining amount of the target value is zero, then complete the liquid extraction.

[0018] Furthermore, in steps S31 and S32, when the two peristaltic pumps are of different sizes, after obtaining the corresponding rounded values, the allocation rule is as follows: if the rounded value is less than the volume of one revolution of the large peristaltic pump, then the volume allocated to both the large and small peristaltic pumps is 1 / 2; if the rounded value is odd, then the large peristaltic pump occupies an extra 1 μL; if it is greater than or equal to the volume of one revolution of the large peristaltic pump, then the large peristaltic pump is allocated first, and the remaining volume is completed by the small peristaltic pump.

[0019] Furthermore, in step S33, after obtaining the percentile integer value, the minimum precision is extracted, the number of minimum precision values ​​is calculated, and one motor is controlled to intake air and the other to exhaust air. At this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, thus completing one extraction. After repeating multiple times, the liquid extraction is completed.

[0020] Furthermore, in step S4, after calculating the minimum precision quantity, one motor is controlled to intake air and the other to exhaust air. At this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, thus completing one extraction. After repeating this process multiple times, the liquid extraction work is completed.

[0021] Compared with existing technologies, this invention has the following advantages: This wide-range automatic and precise control pipette dispensing method, relying on a dual peristaltic pump combination mode, covers a wide range of dispensing needs without the need for multiple pipettes, saving costs and space; through integer allocation plus high-precision iteration with residual volume, the minimum accuracy reaches 0.01μL, accurately adapting to non-integer volume requirements; it provides three modes: automatic, high-precision, and manual, adapting to different usage scenarios and lowering the operation threshold; it reduces manual contact, reduces the risk of liquid contamination and volatilization, and is suitable for precise dispensing in multiple fields. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the hardware design structure in an embodiment of the present invention.

[0023] Figure 2 This is a schematic flowchart of a pipette liquid preparation method with large-scale automatic and precise control in an embodiment of the present invention;

[0024] In the diagram: 1. Peristaltic pump; 2. Hose; 3. Air chamber. Detailed Implementation

[0025] The embodiments of the present invention are described in detail below. These embodiments are implemented based on the technical solution of the present invention, and provide detailed implementation methods and specific operation processes. However, the scope of protection of the present invention is not limited to the following embodiments.

[0026] Example 1

[0027] like Figure 1 As shown, this embodiment provides a technical solution: a pipette structure, whose hardware design mainly includes two peristaltic pumps and an air chamber. When liquid needs to be drawn, the rotation speed of the two peristaltic pumps is controlled by an algorithm to precisely control the amount of air expelled each time. During liquid drawing, there are multiple combination modes depending on the size of the pumps. For example, if the two pumps are of the same size, one rotation will extract 10 μL of the target liquid. If the target value is 10.1 μL, the rotation speed and direction of the two pumps are adjusted, with one pump expelling air and the other inhaling air. One pump rotates forward one revolution (expelling air), while the other rotates in the opposite direction 0.99 revolutions (inhaling air), entering the precise extraction mode, extracting 0.1 μL of the target liquid each time until the target value is reached. By controlling the rotation speed of the two pumps, the minimum extraction value can be adjusted. Compared to current pipettes that require manual operation and multiple pipettes with different volume ranges, this method only requires a single pipette to solve the problem of different volume ranges, and it offers high precision and automatic control.

[0028] like Figure 2As shown, this embodiment also provides a technical solution: a large-range automatic and precise control method for pipette liquid preparation. First, the target value is obtained (generally by direct input or voice input). The target value is then decomposed. Taking 10.1 μL as an example, it is first rounded down to 10 μL. At this time, the motor is controlled to make each of the two pumps rotate half a turn, thus drawing 10 μL of liquid. Then, a high-precision mode is adopted, and the number of pump rotations and repetitions are obtained by dividing the margin value by the minimum precision value. At this time, one pump motor is controlled to rotate forward 1 turn, and the other pump rotates in the reverse direction 0.99 turns, repeating once to obtain the final target value. If the margin is 0.5, then it is repeated 5 times.

[0029] Example 2

[0030] In this embodiment, it is assumed that the two existing peristaltic pumps have an accuracy of 0.01 μL, and the pumps can draw 10 μL and 5 μL of liquid per revolution, respectively. Now, it is necessary to draw 8.88 μL of liquid, 9.88 μL of liquid, and 11.0 μL of liquid.

[0031] 1: The pipetting volumes obtained were 8.88 μL, 9.88 μL, and 11 μL.

[0032] 2: Select one of the three calculation modes, assuming it is automatic mode (the principle of automatic mode is that both pumps should be used, while manual mode can be used if only one pump is used).

[0033] 2-1: First, round down the target value of 8.88 (this rounding doesn't mean taking the exact integer, but rather rounding down each place value; for 8.88, this means rounding down three times: 8, 0.8, and 0.08). This gives a result of 8 μL. Since this is an even number, it's allocated as 4 μL and 4 μL respectively. Similarly, if 9.88 μL is rounded down to 9 μL, it will be allocated as 5 μL and 4 μL. If it's 11 μL, it will be allocated as 10 μL and 1 μL. The allocation rule is based on the following: if the liquid volume is less than the volume of one revolution of the large pump, both the large and small pumps receive half the volume. If the volume is odd, the large pump receives an extra 1 μL. If the volume is greater than or equal to the volume of one revolution of the large pump, the large pump receives the first portion, and the remaining volume is handled by the small pump (if the pump volumes are different).

[0034] 2-2: After the above operations, the integer part has been allocated. Now there is 0.88μL of liquid. We still round it to 0.8μL. After allocating to both sides, there are 0.4μL respectively. That is, we need to rotate 0.04 times and 0.08 times respectively. After reaching the above position, extract 0.01μL. At this time, according to the motor control, one motor is for air intake and the other is for air exhaust. At this time, the large pump rotates 0.999 times for air intake and the small pump needs to rotate 2 times for air exhaust. This completes one extraction. Repeat 8 times to complete the liquid extraction.

[0035] 2-3: After rounding up repeatedly, the number of circles is allocated, the remainder is 0, and the calculation ends.

[0036] 3; Select high precision mode

[0037] If the minimum precision mode is used directly, that is, if it is 8.88μL, the minimum precision will be calculated first, which is 0.01μL. At this time, the rotation amount of the two pumps is calculated according to 0.01μL, that is, the small pump rotates 2 times to exhaust air and the large pump rotates 0.999 times to intake air. Then the number of rotations is calculated, and the two pumps will rotate according to the number of rotations.

[0038] 4: Manual Mode

[0039] This part mainly involves the user inputting the number of revolutions and direction of the two pumps, and the machine controls the pumps to rotate according to the input number of revolutions and direction.

[0040] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A method for large-scale, automated, and precise control of pipette liquid preparation, characterized in that, Includes the following steps: S1: Obtain the volume of the liquid to be prepared as the target value; S2: Select the working mode, which includes automatic mode, high precision mode and manual mode; S3: When the working mode is set to automatic mode, the target value is rounded down multiple times to obtain the corresponding rounded value. Based on each rounded value and according to the liquid volume of each peristaltic pump, the rotation volume of each pump is allocated until the remaining amount of the target value is zero, and the liquid extraction is completed. S4: When the working mode is selected as high precision mode, the minimum precision is directly calculated based on the target value. The rotation amount of each pump is allocated according to the minimum precision quantity and the liquid volume of each peristaltic pump per revolution. Liquid collection is completed after the rotation ends. S5: When the working mode is selected as manual mode, it is used to directly input the number of rotations and direction of the two peristaltic pumps. The two peristaltic pumps will rotate according to the input number of rotations and direction, and the liquid collection will be completed after the rotation is finished. In step S3, the specific processing procedure is as follows: S31: First, round the target value down. Then, based on the rounded integer value, distribute the rotation of each pump according to the liquid volume of each peristaltic pump. S32: Continue to round down the remaining value of the target value to the tenths place. Based on the tenths place value, continue to repeatedly distribute the rotation amount of each pump according to the single-turn liquid volume of the two peristaltic pumps. S33: Continue to round down the remaining value of the target value to obtain the percentile value. Based on the percentile value, continue to repeatedly distribute the rotation amount of each pump according to the single-turn liquid volume of the two peristaltic pumps. S34: Continue taking liquid until the remaining amount of the target value is zero; In steps S31 and S32, when the two peristaltic pumps are of different sizes, after obtaining the corresponding rounded values, the allocation rule is as follows: if the rounded value is less than the volume of one revolution of the large peristaltic pump, then the volume allocated to both the large and small peristaltic pumps is 1 / 2; if the rounded value is odd, then the large peristaltic pump occupies an extra 1 μL; if it is greater than or equal to the volume of one revolution of the large peristaltic pump, then the large peristaltic pump is allocated first, and the remaining volume is completed by the small peristaltic pump. In step S33, after obtaining the percentile integer value, the minimum precision is extracted, the number of minimum precision values ​​is calculated, and one motor is controlled to intake air and the other to exhaust air. At this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, thus completing one extraction. After repeating multiple times, the liquid extraction is completed.

2. The method for large-scale automatic and precise control of pipette liquid preparation according to claim 1, characterized in that, The pipette includes two peristaltic pumps and an air chamber. The two peristaltic pumps are connected to the air chamber through a hose. The air chamber has an inlet at the bottom. The two peristaltic pumps are controlled by motors. A pipette tip is installed on the inlet.

3. The method for large-scale automatic and precise control of pipette liquid preparation according to claim 1, characterized in that, In step S1, the target value is obtained by direct manual input or voice input.

4. The method for large-scale automatic and precise control of pipette liquid preparation according to claim 1, characterized in that, In step S4, after calculating the minimum precision quantity, one motor is controlled to intake air and the other to exhaust air. At this time, the large peristaltic pump rotates m times to intake air and the small peristaltic pump needs to rotate n times to exhaust air, thus completing one extraction. After repeating multiple times, the liquid extraction work is completed.

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