Method for detecting CAR-T (Chimeric Antigen Receptor-T) cells by combining microfluidics and immunofluorescence

By combining immunomagnetic bead enrichment with microfluidic chip partitioning and three-channel fluorescent labeling, the problem of insufficient sensitivity and operational complexity in detecting CAR-T cell concentration in vivo has been solved, achieving highly sensitive quantitative analysis of CAR-T cells and supporting precise management of CAR-T cell therapy.

CN121577883APending Publication Date: 2026-02-27SHENZHEN TIANSHUO BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511964979.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-24
Publication Date
2026-02-27

AI Technical Summary

Technical Problem

Existing methods for detecting CAR-T cell concentration in vivo are not sensitive enough, are complicated to operate, and lack unified monitoring standards and threshold settings, which limits the precision management and personalized treatment optimization of CAR-T cell therapy.

Method used

CAR-T cells were isolated from blood samples using immunomagnetic bead enrichment and microfluidic chip partitioning. Then, three-channel fluorescent labeling with labeled antigens, anti-TCR-PE, and DAPI was performed to achieve automated quantitative analysis of immunofluorescence imaging.

Benefits of technology

It achieves highly sensitive quantitative analysis of CAR-T cell number, cell morphology and function, with a detection limit of 0.001%, supporting the precision management of CAR-T cell therapy.

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Abstract

The invention relates to the field of CAR-T cell concentration detection, in particular to a method for detecting CAR-T cells through combination of microfluidics and immunofluorescence. Through immunomagnetic bead enrichment and micro-fluidic chip partitioning, CAR-T cells are separated from a blood sample through immunomagnetic beads, then labeling is carried out or enhanced by adding a labeling antigen, anti-TCR-PE and DAPI, three-channel fluorescence labeling is achieved, quantitative analysis can be carried out on the number, the cell morphology and the function of the CAR-T cells through automatic immunofluorescence imaging, and the accuracy of CAR-T cell detection is improved. And the detection limit reaches 0.001%.
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