High-speed scanning method for acquiring large-depth-of-field high-definition digital slice

Abstract

The invention discloses a high-speed scanning method for obtaining a large-depth-of-field high-definition digital slice, and relates to the technical field of high-speed scanning and image processing of digital slices. Aligning all the shot view images by using an image matching algorithm; and the clear parts of the aligned view images on different layers are fused to one image to obtain a panoramic image with a large depth of field. According to the invention, the problems of mechanical complexity, long scanning time and image dislocation caused by sample vibration or displacement which are caused by multiple focusing scanning depending on a high-precision three-dimensional displacement platform in the traditional method are avoided; the scanning speed, the imaging quality and the system stability are improved, and the strict requirements of modern pathological diagnosis for detail definition and large-range focusing consistency are met.

Description

Technical Field

[0001] This invention relates to the field of high-speed scanning and image processing technology for digital slicing, and in particular to a high-speed scanning method for acquiring high-resolution digital slices with large depth of field. Background Technology

[0002] Currently, in digital pathology slide scanners, the improvement in optical resolution and the decrease in depth of field are essentially a trade-off between the physical parameters of the optical system. According to classical microscopic optics theory, there is a significant negative correlation between optical resolution and depth of field, a phenomenon particularly prominent in digital pathology slide scanners. To achieve greater depth of field while maintaining resolution, existing technologies mainly employ the following methods: scanning images at different focal planes multiple times and using software algorithms to fuse multiple images into a panoramic image, thereby expanding the effective depth of field; or increasing the depth of field by reducing the numerical aperture of the objective lens or condenser lens.

[0003] Currently, existing technologies have many problems. They require multiple scans and rely on high-precision electric three-dimensional displacement platforms. Small vibrations or displacements of the sample can cause image misalignment, affecting the fusion effect. Low numerical apertures sacrifice resolution, which contradicts the current demand for high resolution and cannot meet the detail requirements of pathological diagnosis. Summary of the Invention

[0004] In view of the problems existing in the high-speed scanning methods for acquiring high-resolution digital slices with large depth of field, this invention is proposed.

[0005] Therefore, the problem to be solved by the present invention is that the present invention solves the problem that existing digital pathological slide scanning requires multiple scans, relies on a precision displacement platform, or sacrifices image resolution due to the difficulty in balancing high resolution and large depth of field.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: In a first aspect, embodiments of the present invention provide a high-speed scanning method for acquiring high-definition digital slices with large depth of field, comprising tilting the slice at an angle so that the slice forms an angle with the horizontal plane and capturing a field-of-view image; Based on the captured field-of-view images, an image matching algorithm is used to align all captured field-of-view images. Using a multifocal image fusion algorithm, the clear parts of the field-of-view images aligned at different levels are fused into a single image to obtain a panoramic image with a large depth of field.

[0007] As a preferred embodiment of the high-speed scanning method for acquiring high-definition digital slices with large depth of field according to the present invention, the high-speed scanning includes acquiring high-definition digital slices with large depth of field using a scanning system, wherein the scanning system comprises an area array camera, a tube lens, an objective lens, a condenser lens and a light source.

[0008] As a preferred embodiment of the high-speed scanning method for acquiring large depth-of-field high-definition digital slices according to the present invention, wherein: the angle between the slice and the horizontal plane is . The pixel size of the area array camera is The number of pixels is w*h, and the optical magnification is [missing information]. The slice holds an angle with the horizontal plane. 0° < ≤5°; The captured field-of-view image includes information from multiple focal planes along the vertical direction of the slice. Therefore: By aligning the scanning direction perpendicular to the width direction of the area array camera, the pixel size is obtained as follows: / .

[0009] As a preferred embodiment of the high-speed scanning method for acquiring large depth-of-field high-definition digital slices according to the present invention, wherein: the scanning direction includes single-view imaging based on the scanning direction, and the formula for calculating the depth-of-field range that can be captured by a single view is:

[0010] in, This indicates the angle between the slice and the horizontal plane, which is a sinusoidal value. This indicates the height of the image sensor in the area array camera. This indicates the pixel size of an area array camera. Indicates optical magnification.

[0011] As a preferred embodiment of the high-speed scanning method for acquiring high-definition digital slices with large depth of field according to the present invention, wherein: the information obtained by capturing multiple focal planes in the vertical direction of the slice includes calculating the step size of the depth of field range for each capture based on the depth of field range that can be captured by a single field of view, and the formula for the step size is:

[0012] in, This indicates the step size of the depth of field for each shot. Indicates the desired number of scan layers; At this point, the number of scanned layers This means that a complete single-view image needs to be segmented into The same physical location on the same layer will not be allowed. Image fusion.

[0013] As a preferred embodiment of the high-speed scanning method for obtaining large depth-of-field high-definition digital slices according to the present invention, the step of aligning all captured field-of-view images using an image matching algorithm includes the following steps: Let the number of field-of-view images acquired after shooting be , If the field of view number is , then the field of view range of the field of view number is . Then the first The first perspective and the second The fixed offset of each field of view in the scanning direction is: Pixel; Use image matching algorithm to calculate the first The first perspective and the second The offset between each field of view is denoted as ; Each field of view is divided into sections perpendicular to the scanning direction. Part, No. The first perspective Record as ,but , , As a group, a group of images according to Perform offset correction.

[0014] As a preferred embodiment of the high-speed scanning method for acquiring large depth-of-field high-definition digital slices according to the present invention, the multi-focal image fusion algorithm includes performing fusion algorithm processing on each group of multi-focal images separately, and the resulting multi-focal image is denoted as... ; The step of performing fusion algorithm processing on each group of multifocal images includes calculating the fusion result using a linear weighted average, and the calculation formula is as follows:

[0015] in, This represents the merged image. This represents the previous set of fused images. This represents the second set of fused images. Indicates the weighting coefficient; After all the images in the group have been fused, a single image fusion result is obtained.

[0016] Secondly, embodiments of the present invention provide a high-speed scanning system for acquiring high-definition digital slices with large depth of field, comprising: a tilting slice imaging module, which tilts the slice at an angle so that the slice forms an angle with the horizontal plane and captures a field-of-view image; an image alignment processing module, which aligns all captured field-of-view images using an image matching algorithm based on the captured field-of-view images; and a multi-focus fusion imaging module, which uses a multi-focus image fusion algorithm to fuse the clear parts of the aligned field-of-view images at different levels into a single image to obtain a panoramic image with large depth of field.

[0017] Thirdly, embodiments of the present invention provide a computer device, including a memory and a processor, wherein the memory stores a computer program, and the processor executes the computer program to implement any step of the above-described high-speed scanning method for acquiring large depth-of-field high-definition digital slices.

[0018] Fourthly, embodiments of the present invention provide a computer-readable storage medium having a computer program stored thereon, wherein: when the computer program is executed by a processor, it implements any step of the above-described high-speed scanning method for acquiring large depth-of-field high-definition digital slices.

[0019] The beneficial effects of this invention are as follows: By placing the pathological slide at a certain angle, this invention can acquire information from multiple focal planes in the vertical direction within a single field of view during a single scan. This avoids the mechanical complexity, long scanning time, and image misalignment caused by sample vibration or displacement resulting from multiple focusing scans using a high-precision three-dimensional displacement platform, which is a problem in traditional methods. At the same time, it does not require reducing the numerical aperture of the objective lens, thus effectively expanding the depth of field while maintaining high optical resolution. Combined with subsequent image matching and multifocal fusion algorithms, it can efficiently and accurately synthesize a large depth-of-field, high-definition digital pathological panoramic image, significantly improving scanning speed, imaging quality, and system stability, and better meeting the stringent requirements of modern pathological diagnosis for detail clarity and wide-range focusing consistency. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein: Figure 1 This is a schematic diagram of an area scan camera used in a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, as provided in an embodiment of the present invention.

[0021] Figure 2 This is a schematic diagram of an objective lens for a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, provided in an embodiment of the present invention.

[0022] Figure 3 This is a schematic diagram of the focal length and scanning direction of a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, provided in an embodiment of the present invention.

[0023] Figure 4 This is an image fusion flowchart of a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, provided as an embodiment of the present invention.

[0024] Figure 5This image shows the effect before image fusion of a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, as provided in an embodiment of the present invention.

[0025] Figure 6 This is an image fusion result of a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, provided in an embodiment of the present invention.

[0026] Figure 7 This is a schematic diagram of the structure of a medium used in a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, as provided in an embodiment of the present invention.

[0027] Figure 8 This is a schematic diagram of a computing device for a high-speed scanning method for acquiring large depth-of-field high-definition digital slices, as provided in an embodiment of the present invention. Detailed Implementation

[0028] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of the present invention.

[0029] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0031] This invention is described in detail with reference to the schematic diagrams. When detailing the embodiments of this invention, for ease of explanation, the cross-sectional views illustrating the device structure may be partially enlarged, not adhering to the usual scale. Furthermore, the schematic diagrams are merely examples and should not be construed as limiting the scope of protection of this invention. In actual fabrication, the three-dimensional spatial dimensions of length, width, and depth should be included.

[0032] Furthermore, in the description of this invention, it should be noted that the terms "upper," "lower," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are used solely for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. In addition, the terms "first," "second," or "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0033] Unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" in this invention should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; similarly, they can refer to mechanical connections, electrical connections, or direct connections, or indirect connections through an intermediate medium, or internal connections between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0034] Example Reference Figures 1-4 This is the first embodiment of the present invention, which provides a high-speed scanning method for acquiring large depth-of-field high-resolution digital slices, including: S1: Tilt the slice at an angle so that it forms an angle with the horizontal plane and capture the field of view image.

[0035] High-speed scanning includes acquiring high-resolution digital slices with large depth of field using a scanning system, which consists of an area array camera, a tube lens, an objective lens, a condenser lens, and a light source.

[0036] The angle between the slice and the horizontal plane is The pixel size of the area scan camera is The number of pixels is w*h, and the optical magnification is [missing information]. ; If capturing a field-of-view image includes information from multiple focal planes along the vertical direction of the slice, then: By aligning the scanning direction perpendicular to the width direction of the area array camera, the pixel size is obtained as follows: / .

[0037] Furthermore, in a specific implementation of the present invention, the pathological slides are first tilted at a certain angle. The slice holds an angle with the horizontal plane. 0° < ≤5°.

[0038] S1.1: The scanning direction includes single-field-of-view imaging based on the scanning direction. The formula for calculating the depth-of-field range that can be captured by a single field of view is:

[0039] in, This indicates the angle between the slice and the horizontal plane, which is a sinusoidal value. This indicates the height of the image sensor in the area array camera. This indicates the pixel size of an area array camera. Indicates optical magnification; the angle between the slice and the horizontal plane. 0° < With an angle ≤5°, the slice is tilted and fixed on the stage, allowing different depth structures of the slice to sequentially enter the focal plane range, thus effectively expanding the depth of field during optical imaging. A scanning system consisting of an area array camera (e.g., 3.45μm pixel size, 4096×3000 pixels resolution), a tube lens, high numerical aperture objectives (e.g., 20× or 40×, corresponding to a total optical magnification of 20 or 40), a condenser lens, and a high-brightness LED light source performs high-speed linear scanning along a direction perpendicular to the camera's width direction to acquire a series of field-of-view images. In this configuration, the actual size of a single pixel on the sample plane is calculated as the pixel size divided by the magnification (e.g., at a magnification of 20, the pixel size is approximately 0.1725μm), while the physical length of a single field of view in the scanning direction is approximately 517.5μm (at a resolution of 3000 pixels). Although the depth of field expansion resulting from a small tilt angle is limited, by combining image matching algorithms and multifocal image fusion techniques, this method can still effectively fuse the sharp portions of field-of-view images aligned at different levels to generate high-definition panoramic images with a large depth of field. This method not only improves scanning efficiency but also reduces the mechanical errors and time consumption that may be caused by traditional Z-axis stack scanning, making it particularly suitable for applications requiring fine resolution.

[0040] S1.2: Information from multiple focal planes in the vertical direction of the slice is obtained by capturing images, including the depth-of-field range that can be captured based on a single field of view. The step size of the depth-of-field range for each capture is calculated, and the formula for the step size is:

[0041] in, This indicates the step size of the depth of field for each shot. Indicates the desired number of scan layers.

[0042] S2: Based on the captured field-of-view images, use an image matching algorithm to align all captured field-of-view images.

[0043] The process of aligning all captured field-of-view images using an image matching algorithm includes the following steps: Let the number of field-of-view images acquired after shooting be , If the field of view number is , then the field of view range of the field of view number is . Then the first The first perspective and the second The fixed offset of each field of view in the scanning direction is: Pixel; Use image matching algorithm to calculate the first The first perspective and the second The offset between each field of view is denoted as ; Each field of view is divided into sections perpendicular to the scanning direction. Part, No. The first perspective Record as ,but , , As a group, a group of images according to Perform offset correction.

[0044] S3: Using a multi-focal image fusion algorithm, the clear parts of the field-of-view images aligned at different levels are fused into a single image to obtain a panoramic image with a large depth of field.

[0045] The multifocal image fusion algorithm includes performing fusion algorithm processing on each group of multifocal images separately, and the resulting multifocal image is denoted as... ; Each group of multifocal images is processed by a fusion algorithm, including calculation using the linearly weighted fusion result. The calculation formula is as follows:

[0046] in, This represents the merged image. This represents the previous set of fused images. This represents the second set of fused images. Indicates the weighting coefficient; After all the images in the group have been fused, a single image fusion result is obtained.

[0047] In a preferred embodiment, a high-speed scanning system for acquiring high-definition digital slices with large depth of field includes a tilting slice imaging module, which tilts the slice at an angle so that the slice makes an angle with the horizontal plane to capture a field-of-view image; an image alignment processing module, which aligns all captured field-of-view images using an image matching algorithm based on the captured field-of-view images; and a multi-focus fusion imaging module, which uses a multi-focus image fusion algorithm to fuse the clear parts of the aligned field-of-view images at different levels into a single image to obtain a panoramic image with large depth of field.

[0048] The above-mentioned unit modules can be embedded in the processor of the computer device in hardware form or independent of it, or they can be stored in the memory of the computer device in software form, so that the processor can call and execute the corresponding operations of the above modules.

[0049] In one embodiment, a computer device is provided, which may be a terminal. The computer device includes a processor, memory, a communication interface, a display screen, and an input device connected via a system bus. The processor of the computer device provides computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and internal memory. The non-volatile storage medium stores an operating system and computer programs. The internal memory provides an environment for the operation of the operating system and computer programs stored in the non-volatile storage medium. The communication interface of the computer device is used for wired or wireless communication with external terminals. Wireless communication can be achieved through Wi-Fi, carrier networks, NFC (Near Field Communication), or other technologies. The display screen of the computer device may be an LCD screen or an e-ink display screen. The input device of the computer device may be a touch layer covering the display screen, or buttons, a trackball, or a touchpad located on the casing of the computer device, or an external keyboard, touchpad, or mouse, etc.

[0050] In summary, this invention, by placing pathological slides at a certain angle, can acquire information from multiple focal planes in the vertical direction within a single field of view during a single scan. This avoids the mechanical complexity, long scanning time, and image misalignment caused by sample vibration or displacement resulting from multiple focusing scans using a high-precision three-dimensional displacement platform, which are inherent problems in traditional methods. Simultaneously, it eliminates the need to reduce the numerical aperture of the objective lens, thus effectively expanding the depth of field while maintaining high optical resolution. Combined with subsequent image matching and multifocal fusion algorithms, it can efficiently and accurately synthesize a large depth-of-field, high-definition digital pathological panoramic image, significantly improving scanning speed, imaging quality, and system stability. This better meets the stringent requirements of modern pathological diagnosis for detail clarity and wide-range focusing consistency.

[0051] After introducing the method and system of exemplary embodiments of the present invention, the following references are made. Figure 3 A computer-readable storage medium according to exemplary embodiments of the present invention will be described, please refer to... Figure 3 The computer-readable storage medium shown is an optical disc 30, on which a computer program (i.e., a program product) is stored. When the computer program is run by a processor, it implements the steps described in the above method implementation, such as tilting the slice at an angle so that the slice makes an angle with the horizontal plane and capturing a field-of-view image; based on the captured field-of-view images, using an image matching algorithm to align all captured field-of-view images; and using a multi-focus image fusion algorithm to fuse the clear parts of the aligned field-of-view images at different levels into a single image to obtain a panoramic image with a large depth of field. The specific implementation methods of each step will not be repeated here.

[0052] It should be noted that examples of computer-readable storage media may also include, but are not limited to, phase-change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), flash memory or other optical and magnetic storage media, which will not be elaborated here.

[0053] After introducing the methods and media of exemplary embodiments of the present invention, the following references are made. Figure 4 A computational device for adaptive recovery of low-voltage power grid self-healing control according to an exemplary embodiment of the present invention.

[0054] Figure 4 A block diagram is shown of an exemplary computing device 40 suitable for implementing embodiments of the present invention. The computing device 40 may be a computer system or a server. Figure 4 The computing device 40 shown is merely an example and should not impose any limitation on the functionality and scope of use of the embodiments of the present invention.

[0055] like Figure 4 As shown, the components of computing device 40 may include, but are not limited to: one or more processors or processing units 401, system memory 402, and bus 403 connecting different system components (including system memory 402 and processing unit 401).

[0056] The computing device 40 typically includes a variety of computer system readable media. These media can be any available media that can be accessed by the computing device 40, including volatile and non-volatile media, and removable and non-removable media.

[0057] System memory 402 may include computer system readable media in the form of volatile memory, such as random access memory (RAM) 4021 and / or cache memory 4022. Computing device 40 may further include other removable / non-removable, volatile / non-volatile computer system storage media. By way of example only, ROM 4023 may be used to read and write non-removable, non-volatile magnetic media (…). Figure 4 Not shown in the image (usually referred to as a "hard drive"). Although not shown in Figure 4The diagram illustrates that disk drives for reading and writing to removable non-volatile disks (e.g., "floppy disks") and optical disc drives for reading and writing to removable non-volatile optical discs (e.g., CD-ROMs, DVD-ROMs, or other optical media) can be provided. In these cases, each drive can be connected to bus 403 via one or more data media interfaces. System memory 402 may include at least one program product having a set (e.g., at least one) of program modules configured to perform the functions of the embodiments of the present invention.

[0058] A program / utility 4025 having a set (at least one) of program modules 4024 may be stored, for example, in system memory 402, and such program modules 4024 include, but are not limited to, an operating system, one or more application programs, other program modules, and program data, each or some combination of these examples may include an implementation of a network environment. Program modules 4024 typically perform the functions and / or methods described in the embodiments of the present invention.

[0059] The computing device 40 can also communicate with one or more external devices 404 (such as a keyboard, pointing device, display, etc.). This communication can be performed via the input / output (I / O) interface 405. Furthermore, the computing device 40 can also communicate with one or more networks (e.g., local area networks (LANs), wide area networks (WANs), and / or public networks, such as the Internet) via a network adapter 406. Figure 4 As shown, network adapter 406 communicates with other modules of computing device 40 (such as processing unit 401) via bus 403. It should be understood that, although... Figure 4 As not shown, it can be used in conjunction with computing device 40 with other hardware and / or software modules.

[0060] The processing unit 401 executes various functional applications and data processing by running programs stored in the system memory 402. For example, it tilts the slice at an angle so that the slice makes an angle with the horizontal plane and captures a field-of-view image; based on the captured field-of-view image, it uses an image matching algorithm to align all captured field-of-view images; and uses a multi-focus image fusion algorithm to fuse the clear parts of the aligned field-of-view images at different levels into a single image to obtain a panoramic image with a large depth of field.

[0061] Those skilled in the art will understand that, for the sake of convenience and brevity, the specific working processes of the systems, devices, and units described above can be referred to the corresponding processes in the foregoing method embodiments, and will not be repeated here.

[0062] In the several embodiments provided by this invention, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. The apparatus embodiments described above are merely illustrative. For example, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. Furthermore, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Additionally, the coupling or direct coupling or communication connection shown or discussed may be through some communication interface; the indirect coupling or communication connection between apparatuses or units may be electrical, mechanical, or other forms.

[0063] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0064] In addition, the functional units in the various embodiments of the present invention can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit.

[0065] If the functionality is implemented as a software functional unit and sold or used as an independent product, it can be stored in a processor-executable, non-volatile, computer-readable storage medium. Based on this understanding, the technical solution of this invention, or the part that contributes to the prior art, or a portion of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods of the various embodiments of this invention. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

[0066] Finally, it should be noted that the above embodiments are merely specific implementations of the present invention, used to illustrate the technical solutions of the present invention, and not to limit it. The scope of protection of the present invention is not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments within the technical scope disclosed in the present invention, or make equivalent substitutions for some of the technical features; and these modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention, and should all be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

[0067] Furthermore, although the operations of the method of the present invention are described in a specific order in the accompanying drawings, this does not require or imply that these operations must be performed in that specific order, or that all the operations shown must be performed to achieve the desired result. Additionally or alternatively, certain steps may be omitted, multiple steps may be combined into one step, and / or one step may be broken down into multiple steps.

[0068] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A high-speed scanning method for acquiring high-resolution digital slices with large depth of field, characterized in that: include, Tilt the slice at an angle so that it forms an angle with the horizontal plane and capture the field of view image; Based on the captured field-of-view images, an image matching algorithm is used to align all captured field-of-view images. Using a multifocal image fusion algorithm, the clear parts of the field-of-view images aligned at different levels are fused into a single image to obtain a panoramic image with a large depth of field.

2. The high-speed scanning method for acquiring large depth-of-field high-resolution digital slices as described in claim 1, characterized in that: The high-speed scanning includes acquiring high-resolution digital slices with large depth of field using a scanning system, which consists of an area array camera, a tube lens, an objective lens, a condenser lens, and a light source.

3. The high-speed scanning method for acquiring large depth-of-field high-resolution digital slices as described in claim 2, characterized in that: The angle between the slice and the horizontal plane is... The pixel size of the area array camera is The number of pixels is w*h, and the optical magnification is [missing information]. The slice holds an angle with the horizontal plane. 0° < ≤5°; The captured field-of-view image includes information from multiple focal planes along the vertical direction of the slice. Therefore: By aligning the scanning direction perpendicular to the width direction of the area array camera, the pixel size is obtained as follows: / .

4. The high-speed scanning method for acquiring large depth-of-field high-resolution digital slices as described in claim 3, characterized in that: The scanning direction includes single-view imaging based on the scanning direction, and the formula for calculating the depth range that can be captured by a single view is: in, This indicates the angle between the slice and the horizontal plane, which is a sinusoidal value. This indicates the height of the image sensor in the area array camera. This indicates the pixel size of an area array camera. Indicates optical magnification.

5. The high-speed scanning method for acquiring large depth-of-field high-definition digital slices as described in claim 4, characterized in that: The information obtained from the shooting of the slice in multiple focal planes along the vertical direction includes calculating the step size of the depth of field range for each shot based on the depth of field range that can be captured by a single field of view. The formula for the step size is: in, This indicates the step size of the depth of field for each shot. Indicates the desired number of scan layers; At this point, the number of scanned layers This means that a complete single-view image needs to be segmented into The same physical location on the same layer will not be allowed. Image fusion.

6. The high-speed scanning method for acquiring large depth-of-field high-definition digital slices as described in claim 5, characterized in that: The process of aligning all captured field-of-view images using an image matching algorithm includes the following steps: Let the number of field-of-view images acquired after shooting be , If the field of view number is , then the field of view range of the field of view number is . Then the first The first perspective and the second The fixed offset of each field of view in the scanning direction is: Pixel; Use image matching algorithm to calculate the first The first perspective and the second The offset between each field of view is denoted as ; Each field of view is divided into sections perpendicular to the scanning direction. Part, No. The first perspective Record as ,but , , As a group, a group of images according to Perform offset correction.

7. The high-speed scanning method for acquiring large depth-of-field high-resolution digital slices as described in claim 6, characterized in that: The multifocal image fusion algorithm includes performing fusion algorithm processing on each group of multifocal images separately, and the resulting multifocal images are denoted as follows: ; The step of performing fusion algorithm processing on each group of multifocal images includes calculating the fusion result using a linear weighted average, and the calculation formula is as follows: in, This represents the merged image. This represents the previous set of fused images. This represents the second set of fused images. Indicates the weighting coefficient; After all the images in the group have been fused, a single image fusion result is obtained.

8. A high-speed scanning system for acquiring high-resolution digital slices with large depth of field, based on the high-speed scanning method for acquiring high-resolution digital slices with large depth of field as described in any one of claims 1 to 7, characterized in that: include, The tilting slice imaging module tilts the slice at an angle so that the slice makes an angle with the horizontal plane and captures the field of view image; The image alignment processing module aligns all captured field-of-view images using an image matching algorithm. The multifocal fusion imaging module uses a multifocal image fusion algorithm to fuse the clear parts of different aligned viewpoint images into a single image, resulting in a panoramic image with a large depth of field.

9. A computer device comprising a memory and a processor, wherein the memory stores a computer program, characterized in that: When the processor executes the computer program, it implements the steps of the high-speed scanning method for acquiring large depth-of-field high-definition digital slices as described in any one of claims 1 to 7.

10. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by the processor, it implements the steps of the high-speed scanning method for acquiring large depth-of-field high-definition digital slices as described in any one of claims 1 to 7.

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