Bladder cancer detection reagent and application thereof

By designing specific primer combinations to detect METTL3 gene expression and combining them with real-time PCR technology, we provide bladder cancer detection reagents and kits, solving the problem of difficult early diagnosis of bladder cancer and achieving highly sensitive early screening and monitoring.

CN121629050APending Publication Date: 2026-03-10HUNAN PROVINCIAL PEOPLES HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-26
Publication Date
2026-03-10

AI Technical Summary

Technical Problem

Bladder cancer often presents with subtle early symptoms, develops rapidly, and is difficult to diagnose due to pathological findings. As a result, most patients are diagnosed at an advanced stage, and the recurrence rate is high. Current technology lacks effective early screening or prognostic monitoring methods.

Method used

We designed specific primer combinations to detect the expression level of the METTL3 gene, and combined them with real-time PCR technology to detect the m6A methylation modification process in bladder cancer samples, providing bladder cancer detection reagents and kits for auxiliary diagnosis.

Benefits of technology

It achieves specific and highly sensitive detection of bladder cancer, with strong anti-interference ability, enabling early screening and monitoring of bladder cancer, reducing diagnostic difficulties and recurrence rates.

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Abstract

The invention discloses a bladder cancer detection reagent and application thereof. The detection reagent comprises a primer combination for detecting the expression level of METTL3; the primer combination comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2. According to the bladder cancer detection reagent provided by the scheme of the invention, a primer combination is obtained by performing primer design on a key gene METTL3 in an m6A methylation modification process, and the primer combination can effectively detect the expression level of the METTL3 gene in a bladder cancer sample, and is high in specificity, high in sensitivity and good in anti-interference performance.
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