Burn and scald spray and preparation method thereof

Abstract

The invention belongs to the technical field of medicines for burns and scalds, and particularly relates to a spray for burns and scalds and a preparation method thereof, the spray for burns and scalds comprises pollen typhae, herba cepbalanoplosis segeti, callicarpa nudiflora, humifuse euphorbia herb, verbena, herba seu stipulae, lalang grass rhizome, a natural astringent, azone, bisabolol and a plant extract. The preparation method comprises the following steps: removing impurities from the herba cepbalanoplosis segeti, callicarpa nudiflora, humifuse euphorbia herb, verbena officinalis, herba seu stigmae draconis and lalang grass rhizome, cleaning, drying and crushing, cleaning and drying the pollen typhae, and soaking the pollen typhae in alcohol; filtering to obtain a liquid medicine, adding borneol into the liquid medicine, then adding glycerol, azone, a plant extract, bisabolol and a natural astringent, and diluting the alcohol concentration in the liquid medicine with sterile distilled water; the spray for burns and scalds provided by the invention can timely treat wound surfaces and quickly stop bleeding, and has the characteristics of safety, no irritation, economy, convenience, natural healing, no scars and the like.

Description

Technical Field

[0001] This invention belongs to the field of burn and scald medicine technology, specifically relating to a burn and scald spray and its preparation method. Background Technology

[0002] Following burns, especially large-area burns, the most common complications are painful shock and increased permeability of capillaries in and around the burn wound. If large amounts of plasma-like fluid are not controlled promptly, this can lead to hypovolemic shock. Western medicine's main emergency treatment measures for severe burns include: rapidly replenishing lost fluids, restoring effective circulation, and improving tissue perfusion. Treatment methods generally include full bandaging, partial bandaging, and moist wound dressing. All three methods require bandaging or partial bandaging, and each dressing change inevitably damages the wound, hindering healing. This also causes significant patient discomfort and, more importantly, fails to effectively control wound fluid exudation and pain. Furthermore, the excessive use of antibiotics can fail to control local infection and wound fluid extravasation, potentially leading to sepsis.

[0003] While traditional Chinese medicine formulas for treating burns are widely used in my country, their medicinal components and proportions vary, resulting in inconsistent therapeutic effects. Consequently, there are very few traditional Chinese medicine burn treatments that are rationally formulated, advanced in preparation, of controllable quality, and reliable in efficacy.

[0004] Existing technologies using traditional Chinese medicine formulas, such as application number CN201210253148.7, which describes a cancer-inhibiting and tissue-regenerating drug for treating burns, scalds, internal and external ulcers, and cataracts, and its preparation method, all use traditional Chinese medicinal materials and have no side effects. However, the drug penetration is slow, and the wound healing time is long. Existing technologies also include those that promote rapid healing, such as application number CN03105303.3, which describes a traditional Chinese medicine spray for treating burns. This spray uses high-concentration ethanol to soak traditional Chinese medicine. Although it is very effective for burn wounds, the high-concentration ethanol solution is highly irritating to the skin, causing short-term pain. Due to individual differences, some patients may be allergic to ethanol, resulting in rashes, erythema, etc. Long-term use of ethanol for skin disinfection can lead to degreasing, dryness, and even roughness of the skin. Summary of the Invention

[0005] To address the problems in the prior art, this invention provides a burn spray and its preparation method, which enables timely treatment of wounds, rapid hemostasis, and rapid wound healing while reducing irritation. It is safe, non-irritating, economical, convenient, and promotes natural and rapid healing without leaving scars.

[0006] The technical problem solved by this invention is achieved by the following technical solution: The purpose of this invention is to provide a burn spray, comprising cattail pollen, thistle, callicarpa nudiflora, euphorbia humifusa, verbena, sedge, imperata cylindrica, natural astringent, azone, and plant extracts.

[0007] Further preferred options include bisabolol.

[0008] Further preferred, the natural astringent is palm charcoal extract.

[0009] Further preferred plant extracts include motherwort extract, centella asiatica extract, and hypericum hydropiper extract.

[0010] Further preferred formulations, by weight, include: 4-10 parts of Typha pollen, 2-6 parts of Cirsium japonicum, 1-5 parts of Callicarpa nudiflora, 1-5 parts of Euphorbia humifusa, 3-8 parts of Verbena officinalis, 2-8 parts of Cirsium japonicum, 1-4 parts of Imperata cylindrica root, 1-10 parts of palm charcoal extract, 0.5-4 parts of bisabolol, 1-5 parts of azone, 0.5-2 parts of Leonurus japonicus extract, 0.1-1 parts of Centella asiatica extract, and 0.1-5 parts of Hypericum perforatum water extract.

[0011] Further preferably, the mixture also includes 3-5 parts of borneol, 4-8 parts of glycerin, and 10-40 parts of 70% alcohol by weight.

[0012] A method for preparing a burn spray involves removing impurities from *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Herba Caryophyllida*, and *Imperata cylindrica*, washing, drying, and pulverizing them. *Typha pollen* is washed and dried. The resulting pulverized *Typha pollen*, *Cirsium japonicum*, *Euphorbia humifusa*, *Verbena officinalis*, *Herba Caryophyllida*, and *Imperata cylindrica* are then soaked in alcohol. The solution is filtered, and borneol is added. Glycerin, azone, plant extracts, bisabolol, and a natural astringent are then added. The alcohol concentration in the solution is diluted with sterile distilled water. The solution is then dispensed and sealed.

[0013] Further preferred methods involve soaking crushed cattail pollen, crushed small thistle, crushed pearl bark, crushed euphorbia humifusa, crushed verbena, crushed tiger beard grass, and crushed imperata root in alcohol for 10-15 days, and then diluting the alcohol concentration in the solution with sterile distilled water to 20-30%.

[0014] A further preferred method for cleaning, washing, drying, and pulverizing *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Heliotropium indicum*, and *Imperata cylindrica* includes: removing impurities from *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Heliotropium indicum*, and *Imperata cylindrica*, soaking them in a washing and moistening tank with water 3-5 cm above the herbs for 3-5 hours, then allowing them to remain moist for another 3-5 hours. Afterward, cut the herbs into small, uniform pieces or slices; dry them in an oven at 40-60 degrees Celsius with a conveyor belt speed of 0.3-0.5 meters per minute; after drying, cool to room temperature and pulverize using a pulverizer to obtain pulverized *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Heliotropium indicum*, and *Imperata cylindrica*. The pulverizer mesh size is adjusted to 2-4 millimeters. *Typha orientalis* is already in powder form and does not require pulverization; it only needs to be washed and dried.

[0015] This invention's plant-based formula primarily consists of Typha pollen, Cirsium japonicum, Callicarpa nudiflora, Euphorbia humifusa, Verbena officinalis, Smilax china, and Imperata cylindrica. Smilax china provides antibacterial, anti-inflammatory, and analgesic effects; Typha pollen stops bleeding and disperses blood stasis; Euphorbia humifusa and Verbena officinalis clear heat and detoxify; Cirsium japonicum and Callicarpa nudiflora stop bleeding and relieve pain; and Imperata cylindrica stops bleeding and reduces swelling. Palm charcoal extract provides rapid astringency, and azone acts as a penetration enhancer. Motherwort extract is antibacterial; Centella asiatica extract provides antioxidant benefits, repairs skin, and reduces scar formation; and Hypericum hydropiper extract promotes wound healing. Borneol provides preservative protection, and glycerin moisturizes without drying, preventing the wound from becoming excessively dry.

[0016] Bisabolol has anti-inflammatory and analgesic properties, prevents infection, rapidly promotes wound healing, repairs wound surfaces, regenerates cells, relieves scar-related symptoms, and has an efficacy rate of 92% in relieving scar itching. Through repeated experiments, the addition of bisabolol to this invention has shown significant advantages in anti-inflammatory and analgesic effects, antioxidant properties, antibacterial and anti-infection effects, rapid film formation, moisturizing and repairing, promoting wound healing, relieving scar symptoms, and reducing irritation. Therefore, after using this medicine, it can quickly absorb moisture from the wound and exert the effects of clearing heat and detoxifying, reducing inflammation, stopping bleeding, relieving pain, astringing, and promoting tissue regeneration.

[0017] Furthermore, in this invention, the precise dosage of each ingredient, while working together, can further enhance the efficacy. Through repeated experiments, the optimal wound healing time and effect were achieved when the following ingredients were used: 7 parts of Typha pollen powder, 4 parts of Cirsium japonicum powder, 3 parts of Callicarpa nudiflora powder, 3 parts of Euphorbia humifusa powder, 6 parts of Verbena officinalis powder, 5 parts of Euphorbia tirucalli powder, 2 parts of Imperata cylindrica root powder, 5 parts of palm charcoal extract, 2 parts of bisabolol, 3 parts of azone, 1 part of Leonurus japonicus extract, 0.5 parts of Centella asiatica extract, 2.5 parts of Hypericum perforatum water extract, 4 parts of borneol, 6 parts of glycerin, and 25 parts of 70% alcohol.

[0018] The therapeutic mechanism of this invention's spray is as follows: After application, a protective film quickly forms on the wound, keeping it moist and preventing dryness. This effectively controls fluid leakage, avoids and reduces fluid loss, inhibits bacterial invasion, maintains effective circulation and acid-base balance, does not weaken the body's disease resistance, and avoids damage to new skin caused by bandaging. Therefore, it is highly beneficial for promoting epithelial cell growth. After new skin forms, the medicated film naturally separates, and damaged scabs gradually fall off. For small-area accidental scrapes, the spray can be applied directly to the wound without bandaging, allowing for fully open treatment. After application, the wound quickly stops bleeding, forms a protective film, prevents fluid leakage, inhibits bacterial invasion, and heals within 1-3 days.

[0019] Compared with the prior art, the beneficial technical effects of the present invention are as follows: This invention relates to a plant-based topical spray for treating first-degree, second-degree, and small-area third-degree burns. Employing a fully open treatment approach, it allows for timely wound management and rapid hemostasis. The spray immediately covers and forms a protective film on the wound, effectively controlling bleeding, fluid extravasation, and infection, preventing sepsis. It is safe, non-irritating, economical, convenient, and promotes natural healing without scarring.

[0020] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention, it can be implemented according to the contents of the specification. Furthermore, in order to make the above contents, objectives, features and advantages of the present invention more obvious and understandable, specific embodiments of the present invention are described below. Detailed Implementation

[0021] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanatory of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0022] In addition, unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be obtained by purchasing them from the market or prepared by existing methods.

[0023] This invention relates to a burn spray, a herbal external spray preparation for treating first-degree, second-degree burns, third-degree small-area burns, and accidental scratches. The formulation comprises the following components by weight: 4-10 parts of Typha pollen, 2-6 parts of Cirsium japonicum, 1-5 parts of Callicarpa nudiflora, 1-5 parts of Euphorbia humifusa, 3-8 parts of Verbena officinalis, 2-8 parts of Cirsium japonicum, 1-4 parts of Imperata cylindrica root, 1-10 parts of natural astringent, 0.5-4 parts of bisabolol, 1-5 parts of azone, 0.5-2 parts of Leonurus japonicus extract, 0.1-1 part of Centella asiatica extract, 0.1-5 parts of Hypericum perforatum water extract, 3-5 parts of borneol, 4-8 parts of glycerin, and 10-40 parts of 70% alcohol.

[0024] Preparation method: Remove impurities from the above-mentioned Chinese herbal medicines, wash, dry, and pulverize them to obtain cattail pollen powder, small thistle powder, naked flower purple beauty powder, ground euphorbia powder, verbena powder, tiger beard grass powder, and white grass root powder. Soak them in a container with 70% alcohol for 10-15 days. Filter the liquid, add borneol that has been dissolved in alcohol beforehand to the liquid, and after it is fully dissolved, add glycerin, bisabolol, azone, plant extract, palm charcoal extract, and sterile distilled water to adjust the alcohol concentration of the plant liquid to 20-30%. Dispense the finished product into sterile spray bottles with spray nozzles.

[0025] Usage: After rinsing the burn wound with clean water, absorb the liquid with a sterile cotton ball and quickly spray the medication onto the wound. For completely exposed wound treatment, bandaging is not required. Initially, spray every 20-30 minutes. Once a protective film forms and pain is significantly reduced, spray three times a day (morning, noon, and evening). If infection occurs, pus can be cleaned immediately and medication can be continued. This treatment avoids damage to newly formed granulation tissue and pseudodermis caused by each dressing change and bandaging.

[0026] Note: There may be some pain when spraying the medication on a fresh wound. The pain usually subsides or stops within 5-10 seconds. If the wound is still painful after the medication dries, you can continue spraying to quickly relieve the pain. For completely exposed wound treatment, close observation is essential. After the protective film forms, if there is pus under the scab, remove the scab and clean the pus in time, then continue spraying the medication. Example 1

[0027] A method for preparing a burn spray includes the following steps: 1. Take cattail pollen, thistle, callicarpa nudiflora, euphorbia humifusa, verbena, sedge, and imperata cylindrica and place them in the sorting room in that order. Select herbs that are moldy, insect-eaten, discolored, or oily. After washing the cattail pollen, dry it in a 50°C oven. Place the remaining herbs on the cleaning table and turn on the dust removal device to remove dust. Transfer them to the washing and moistening room, place each herb in a bucket and then transfer it to the washing and moistening pool to soak. The water level should be 3 cm above the herbs. Soak for 4 hours, then let it sit for 3 hours until the herbs are thoroughly soaked. Transfer them to the cutting room. Add each herb to a double slicer and cut it into small pieces or slices of about 1 cm in size. Transfer each herb to the drying room and turn on the microwave drying and sterilization oven. Set the temperature to 50°C and the conveyor belt speed to 0.3 m / min. Spread the dried herbs evenly on stainless steel trays for cooling. Once the herbs have cooled to room temperature, send them to the pulverizing room. Set the pulverizer mesh to 2 mm, turn on the pulverizer and pulverize to obtain small thistle powder, naked flower purple beauty powder, ground euphorbia powder, verbena powder, tiger beard grass powder, and white grass root powder; 2. Weigh out 4 parts of crushed cattail pollen, 2 parts of crushed thistle, 1 part of crushed callicarpa nudiflora, 1 part of crushed creeping euphorbia humifusa, 3 parts of crushed verbena, 2 parts of crushed tiger's beard grass, and 1 part of crushed Imperata cylindrica root, and soak them in 5 parts of 70% alcohol solution for 10 days. Filter the solution through a 120-mesh plate and frame filter to obtain the plant extract. 3. Dissolve 3 parts borneol in 5 parts alcohol to obtain a borneol alcohol solution for later use. During the process, continuously observe whether the borneol is completely dissolved. If it is not completely dissolved, the stirring speed should be increased or the stirring time should be extended. 4. Add 1 part of palm charcoal extract and 0.5 parts of bisabolol to sterile distilled water and stir to homogenize to obtain a film-forming and astringent solution. 5. Add the filtered plant extract to a mixing bowl and start stirring, adjusting the stirring frequency to 50 Hz. Add 4 parts glycerin, 1 part azone, borneol alcohol solution, film-forming astringent solution, 0.5 parts motherwort extract, 0.1 parts centella asiatica extract, 0.1 parts hypericum water extract, and sterile distilled water. The amount of sterile distilled water should be adjusted to control the overall alcohol solution concentration to 20%. Stir for 1.2 hours and then stop stirring. Pass the solution through a 200-mesh filter, take a sample for testing, and after passing inspection, dispense and transport it to the filling workshop. 6. After testing, the solution can be poured into a spray bottle with a spray nozzle to obtain the plant spray for burns and scalds of the present invention. Example 2

[0028] A method for preparing a burn spray includes the following steps: 1. Take cattail pollen, thistle, callicarpa nudiflora, euphorbia humifusa, verbena, sedge, and imperata cylindrica and place them in the sorting room in that order. Select herbs that are moldy, insect-eaten, discolored, or oily. After washing the cattail pollen, dry it in a 40°C oven. Place the remaining herbs on the cleaning table and turn on the dust removal device to remove dust. Transfer them to the washing and moistening room, place each herb in a bucket and then transfer it to the washing and moistening pool to soak. The water level should be 4 cm above the herbs. Soak for 4 hours, then let it sit for another 4 hours until the herbs are thoroughly soaked. Transfer them to the cutting room. Add each herb to a double slicer and cut it into small pieces or slices of about 1 cm in size. Transfer the herbs to the drying room and turn on the microwave drying and sterilization oven. Set the temperature to 40°C and the conveyor belt speed to 0.4 m / min. Spread the dried herbs evenly on stainless steel trays for cooling. Once the herbs have cooled to room temperature, send them to the pulverizing room. Set the pulverizer mesh to 3 mm, turn on the pulverizer and pulverize to obtain small thistle powder, naked flower purple beauty powder, ground euphorbia powder, verbena powder, tiger beard grass powder, and white grass root powder; 2. Weigh out 7 parts of crushed cattail pollen, 4 parts of crushed thistle, 3 parts of crushed Callicarpa nudiflora, 3 parts of crushed Euphorbia humifusa, 6 parts of crushed Verbena officinalis, 5 parts of crushed Euphorbia tirucalli, and 2 parts of crushed Imperata cylindrica root, and soak them in 15 parts of 70% alcohol solution for 12 days. Filter the solution through a 120-mesh plate and frame filter to obtain the plant extract. 3. Dissolve 4 parts of borneol in 10 parts of alcohol to obtain a borneol alcohol solution for later use (method as above). 4. Add 5 parts of palm charcoal extract and 2 parts of bisabolol to sterile distilled water and stir to homogenize to obtain a film-forming and astringent solution; 5. Add the filtered plant extract to a mixing bowl and start stirring, adjusting the stirring frequency to 50 Hz. Add 6 parts glycerin, 3 parts azone, borneol alcohol solution, film-forming astringent solution, 1 part motherwort extract, 0.5 parts centella asiatica extract, 2.5 parts hypericum water extract, and sterile distilled water. The amount of sterile distilled water should be adjusted to control the overall alcohol solution concentration to 25%. Stir for 1.5 hours and then stop stirring. Pass the solution through a 200-mesh filter, take a sample for testing, and after it passes the test, dispense and transport it to the filling workshop. 6. After testing, the solution can be poured into a spray bottle with a spray nozzle to obtain the plant spray for burns and scalds of the present invention. Example 3

[0029] A method for preparing a burn spray includes the following steps: 1. Take cattail pollen, thistle, callicarpa nudiflora, euphorbia humifusa, verbena, sedge, and imperata cylindrica and place them in the sorting room in that order. Select herbs that are moldy, insect-eaten, discolored, or oily. After washing the cattail pollen, dry it in a 60°C oven. Place the remaining herbs on the cleaning table and turn on the dust removal device to remove dust. Transfer them to the washing and moistening room, place each herb in a bucket and then transfer it to the washing and moistening pool to soak. The water level should be 5 cm above the herbs. Soak for 4 hours, then let it sit for 5 hours until the herbs are thoroughly soaked. Transfer them to the cutting room. Add each herb to a double slicer and cut it into small pieces or slices of about 1 cm in size. Transfer the herbs to the drying room and turn on the microwave drying and sterilization oven. Set the temperature to 60°C and the conveyor belt speed to 0.5 m / min. Spread the dried herbs evenly on stainless steel trays for cooling. Once the herbs have cooled to room temperature, send them to the pulverizing room. Set the pulverizer mesh to 4 mm, turn on the pulverizer and pulverize to obtain small thistle powder, naked flower purple beauty powder, ground euphorbia powder, verbena powder, tiger beard grass powder, and white grass root powder; 2. Weigh out 10 parts of Typha pollen, 6 parts of Cirsium japonicum, 5 parts of Callicarpa nudiflora, 5 parts of Euphorbia humifusa, 8 parts of Verbena officinalis, 8 parts of Euphorbia tirucalli, and 4 parts of Imperata cylindrica root, and soak them in 20 parts of 70% alcohol solution for 15 days. Filter the solution through a 120-mesh plate and frame filter to obtain the plant extract. 3. Dissolve 5 parts of borneol in 20 parts of alcohol to obtain a borneol alcohol solution for later use (method as above); 4. Add 10 parts of palm charcoal extract and 4 parts of bisabolol to sterile distilled water and stir to homogenize to obtain a film-forming and astringent solution; 5. Add the filtered plant extract to a mixing bowl and start stirring, adjusting the stirring frequency to 50 Hz. Add 8 parts glycerin, 5 parts azone, 2 parts motherwort extract, 1 part centella asiatica extract, 5 parts hypericum water extract, borneol alcohol solution, film-forming astringent solution, and sterile distilled water. The amount of sterile distilled water should be adjusted to control the overall alcohol solution concentration to 30%. Stir for 2 hours and then stop stirring. Pass the solution through a 200-mesh filter, take a sample for testing, and after it passes the test, dispense and transport it to the filling workshop. 6. After testing, the solution can be poured into a spray bottle with a spray nozzle to obtain the plant spray for burns and scalds of the present invention.

[0030] Comparative Example 1 Comparative Example 1 is a cancer-inhibiting and tissue-regenerating drug for treating burns, internal and external ulcers, and cataracts, based on prior art application number CN201210253148.7.

[0031] A cancer-suppressing and tissue-regenerating medicine for treating burns, scalds, internal and external ulcers, and cataracts, characterized by being prepared from the following raw materials in the indicated weight ratios: Angelica dahurica 25-30 parts, Lithospermum erythrorhizon 15-30 parts, camphor 15-25 parts, and borneol 10 parts. ~15 parts, Sanguisorba officinalis 25-40 parts, Glauber's salt 10-35 parts, Typha pollen 12-30 parts, Nelumbo nucifera rhizome 10-42 parts, Cypress leaf charcoal 10-45 parts, human hair 10-40 parts, Amaranthus trifoliata 12-45 parts, Bletilla striata 10-35 parts, Imperata cylindrica rhizome 10-30 parts, Eclipta prostrata 10-30 parts, Agrimonia pilosa 10-40 parts, Cirsium japonicum 10-40 parts, Cirsium setosum 10-40 parts, Rubia cordifolia 10-35 parts, Dieffenbachia 10-40 parts, Taraxacum mongolicum 1-10 parts, Plantago asiatica 10-40 parts, Lygodium japonicum 10-25 parts, Viola yedoensis 1-30 parts, Lonicera japonica 1-30 parts.

[0032] It is prepared by the following steps: A. According to the weight ratio, take the following raw materials and place them in a heating dish. Heat to 50℃~70℃, stir-fry, and grind to make powder: Angelica dahurica 25~30 parts, Lithospermum erythrorhizon 15~30 parts, Sanguisorba officinalis 25~40 parts, Sodium sulfate 10~35 parts, Typha pollen 12~30 parts, Nelumbo nucifera rhizome 10~42 parts, Cypress leaf charcoal 10~45 parts, human hair charcoal 10~40 parts, Amaranthus trifoliata 12 parts. ~45 parts, Bletilla striata 10~35 parts, Imperata cylindrica 10~30 parts, Eclipta prostrata 10~30 parts, Agrimonia pilosa 10~40 parts, Cirsium japonicum 10~40 parts, Cirsium setosum 10~40 parts, Rubia cordifolia 10~35 parts, Commelina communis 10~40 parts, Taraxacum mongolicum 1~10 parts, Plantago asiatica 10~40 parts, Lygodium japonicum 10~25 parts, Viola yedoensis 1~30 parts, Lonicera japonica 1~30 parts; B. According to the weight ratio, take 15-25 parts of camphor and 10-15 parts of borneol, add them together with the powder made from all the raw materials in step A into the reaction vessel, then add water ten times the total weight of the raw materials in the reaction vessel, heat to 100 degrees, cool and filter to obtain filtrate; then soak the obtained filtrate in cool boiled water ten times its weight, process for 7-15 days, then filter, sterilize, bottle and air dry to obtain aqueous medicine.

[0033] Comparative Example 2 Comparative Example 2 is a plant-based spray for treating burns, based on prior art application number CN03105303.3.

[0034] The following is a combination of medications for burn spray: 300g of *Pterocarya stenoptera* bark, 300g of *Achyranthes bidentata*, 500g of *Scutellaria baicalensis*, 500g of *Phellodendron chinense*, 500g of raw rhubarb, 400g of catechu, 400g of borneol, 800g of glycerin, and 10,000ml of 90% alcohol.

[0035] Preparation method: First, remove the rough skin and impurities from the Chinese herbs and soak them in a container containing 10,000 ml of alcohol for 1 month; filter the solution, add the borneol solution that has been dissolved in alcohol in advance to the medicinal solution, and after it is fully dissolved, add glycerin and sterile distilled water to adjust the alcohol concentration of the plant extract to 60-70%; then decontaminate and package the product into sterile 70 ml bottles to obtain the finished product.

[0036] Comparative Example 3 Unlike Example 2, bisabolol was not added; otherwise, they were the same.

[0037] Comparative Example 4 The ingredients differ from Example 2 in that: 12 parts of crushed cattail pollen, 8 parts of crushed small thistle, 6 parts of crushed golden needle flower, 6 parts of crushed creeping euphorbia, 9 parts of crushed verbena, 9 parts of crushed tiger's beard grass, 5 parts of crushed white grass root, 11 parts of palm charcoal extract, 5 parts of bisabolol, 6 parts of azone, 3 parts of motherwort extract, 2 parts of centella asiatica extract, and 6 parts of water extract of St. John's wort. The rest are the same.

[0038] Experimental Example 1: Quantitative Killing Test of Escherichia coli Suspension Sterilization Test: Test standard: Section 2.1.1.7, 4 of the "Disinfection Technical Specifications" (2002 edition). The test used the sprays from Examples 1-3, with contact times of 1 min, 2 min, and 3 min, and a constant temperature of 20℃. The test was repeated 3 times. Ambient temperature: 22.8℃; relative humidity: 48%.

[0039] Table 1. Killing effect on Escherichia coli

[0040] Note: The negative control showed no sterile growth.

[0041] After three repeated tests under a constant temperature of 20℃, the spray of this invention showed a kill rate of >5.00 against Escherichia coli in the suspension after 1 min, 2 min, and 3 min of application. This meets the disinfection qualification requirements of the "Disinfection Technical Specifications" (2002 edition).

[0042] Experimental Example 2: Quantitative Killing Test of Staphylococcus aureus Suspension Sterilization Test: Test criteria: Section 2.1.1.7, Item 4 of the "Disinfection Technical Specifications" (2002 edition). The test used the sprays from Examples 1-3, with contact times of 1 min, 2 min, and 3 min, and a constant temperature of 20℃. The test was repeated three times. Ambient temperature: 22.8℃; Relative humidity: 48%.

[0043] Table 2 shows the bactericidal effect against Staphylococcus aureus.

[0044] Note: The negative control showed no sterile growth.

[0045] After three repeated tests under a constant temperature of 20℃, the spray of this invention showed a kill logarithmic effect of >5.00 on Staphylococcus aureus in the suspension after 1 min, 2 min, and 3 min of application. This meets the disinfection qualification requirements of the "Disinfection Technical Specifications" (2002 edition).

[0046] Experimental Example 3: Quantitative Killing Test of Pseudomonas aeruginosa Suspension Sterilization Test: Test criteria: Section 2.1.1.7, Item 4 of the "Disinfection Technical Specifications" (2002 edition). The test used the sprays from Examples 1-3, with contact times of 1 min, 2 min, and 3 min, and a constant temperature of 20℃. The test was repeated three times. Ambient temperature: 22.8℃; Relative humidity: 48%.

[0047] Table 3 shows the bactericidal effect against Pseudomonas aeruginosa.

[0048] Note: The negative control showed no sterile growth.

[0049] After three repeated tests under constant temperature conditions of 20℃, the spray of this invention showed a kill logarithmic effect of >5.00 on Pseudomonas aeruginosa in the suspension after 1 min, 2 min, and 3 min. This meets the disinfection qualification requirements of the "Disinfection Technical Specifications" (2002 edition).

[0050] Experimental Example 4: Quantitative Killing Test of Candida albicans Suspension Sterilization Test: Test criteria: Section 2.1.1.7, Item 4 of the "Disinfection Technical Specifications" (2002 edition). The test used the sprays from Examples 1-3, with contact times of 1 min, 2 min, and 3 min, and a constant temperature of 20℃. The test was repeated three times. Ambient temperature: 22.8℃; Relative humidity: 48%.

[0051] Table 4. Killing effect against Candida albicans

[0052] Note: The negative control showed no sterile growth.

[0053] After three repeated tests under a constant temperature of 20℃, the spray of this invention showed a kill rate of >4.00 against Candida albicans in the suspension after 1 min, 2 min, and 3 min of application. This meets the disinfection qualification requirements stipulated in the "Disinfection Technical Specifications" (2002 edition).

[0054] Experimental Example 5: Field Test of Skin Disinfection Experimental method: In accordance with Section 2.1.2.8 of the "Disinfection Technical Specifications" (2002 edition), after thoroughly rubbing the middle of the inner forearms of 30 subjects against each other, a specification board was placed on the surface of the middle of the inner forearm of the subjects' left forearms. A sterile cotton swab was moistened in a test tube containing 10 mL of diluent, squeezed dry on the tube wall, and then rubbed horizontally back and forth 10 times and vertically back and forth 3 times within the area defined by the specification board. After each swab, the cotton swab was rotated once. Using aseptic technique, the sampling end was cut into the original diluent test tube as a positive control group. 3.0 mL of the spray prepared in Example 2 of this invention was used to disinfect the area defined by the specification plate on the right forearm. After 2.0 min of disinfection, a sterile cotton swab was moistened in a test tube containing 10 mL of neutralizing agent solution, and the right forearm was sampled and inoculated using the same method as the experimental group. Unused neutralizing agent and diluent from the same batch were used to inoculate the culture medium as a negative control group. Samples from the experimental group, positive control group, and negative control group were inoculated into Petri dishes, TSA was poured in, and after condensation, the samples were incubated at 37°C for 48 h. The number of surviving colonies was counted, and the kill log value was calculated. The results are shown in Table 5 below.

[0055] Table 5 Results of field tests for skin surface disinfection

[0056] Note: Negative control showed no sterile growth. Residual bacterial count after skin disinfection: 0.00 CFU / cm³ 2 -1.36 CFU / cm 2 .

[0057] Testing revealed that, under ambient temperatures of 22.6℃ and relative humidity of 47%, the spray of this invention, after 2.0 minutes of disinfection, achieved an average log reduction of 1.86 against naturally occurring bacteria on skin samples from 30 subjects, ranging from 1.06 to 3.22. The average log reduction of naturally occurring bacteria was >1.00, and the residual bacterial count after skin disinfection was <5.0 CFU / cm³. 2 It meets the requirements for qualified disinfection as stipulated in the "Disinfection Technical Specifications" (2002 edition).

[0058] Experimental Example 6: Microbial Contamination Indicators 1. Testing basis: Appendix A of GB27951-2021 "General Requirements for Skin Disinfectants" 2. Sample preparation: Take 5.0 mL of the spray prepared in Example 2 of this invention and add it to a TPS solution containing 45.0 mL of neutralizing agent. Mix well to prepare a 1-10 dilution test solution. Then, continue to dilute with TPS solution by 10 times to prepare a 1:100 test solution and a 1:1000 test solution.

[0059] 3. Total colony count determination: Take 1:10 and 1:100 test solutions and inoculate 1 mL onto each Petri dish. Inoculate two sterile Petri dishes for each dilution. Use an empty Petri dish without sample as a negative control. Pour nutrient agar medium onto each Petri dish and, after solidification, incubate at 36℃ for 48 hours.

[0060] 4. Mold and yeast assay: Take 1:10, 1:100, and 1:1000 dilutions of the test solution and inoculate 1 mL onto each Petri dish. Inoculate two sterile Petri dishes for each dilution. Use an empty Petri dish without sample as a negative control. Pour nutrient agar medium onto each Petri dish and, after solidification, incubate at 28°C for 72 hours.

[0061] 5. Detection of pathogenic pyogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, and beta-hemolytic streptococci): Take 10 mL of the 1:10 test solution from step 2 above, enrich and culture the bacteria, and then perform streak isolation culture. When suspicious colonies appear, perform corresponding biochemical identification and make a judgment based on the typical characteristics of each bacterium.

[0062] 6. Ambient temperature: 22.6℃, relative humidity: 48%.

[0063] Table 6 Results of Microbial Contamination Indicators Detection

[0064] Note: The negative control showed no sterile growth.

[0065] Testing revealed that the total bacterial count of microbial contamination in the spray of this invention was <10 CFU / mL, and the levels of molds and yeasts were <10 CFU / mL. Staphylococcus aureus, Pseudomonas aeruginosa, and beta-hemolytic streptococci were not detected. This meets the requirements for microbial contamination stipulated in GB 27951-2021 "General Requirements for Skin Disinfectants".

[0066] Experimental Example 7: Sterility Test 1. Testing basis: Appendix A of GB27951-2021 "General Requirements for Skin Disinfectants".

[0067] 2. Sample preparation: Take 5.0 mL of the spray prepared in Example 2 of this invention, add it to a TPS solution containing 45.0 mL of neutralizing agent, mix well, and prepare a 1:10 dilution solution for testing.

[0068] 3. Sterility Test: 7 mL of the test solution was inoculated into 5 aerobic-anaerobic culture tubes and 2 fungal culture tubes. One of the aerobic-anaerobic culture tubes containing the sample was inoculated with 1.0 mL of diluted Staphylococcus aureus suspension as a positive control. One aerobic-anaerobic culture tube and one fungal culture tube were placed on the test bench with the caps open until the sample sterility test was completed. The caps were then closed, and the tubes were incubated together with the test sample as a negative control. The aerobic-anaerobic culture tubes, positive control tubes, and negative control tubes inoculated with the disinfectant dilution were simultaneously placed in a 30°C incubator for 5 consecutive days, with daily observation of the results. The fungal culture tubes, positive control tubes, and negative control tubes inoculated with the disinfectant dilution were simultaneously placed in a 25°C incubator for 7 consecutive days, with daily observation of the results.

[0069] 4. Ambient temperature: 22.6℃, relative humidity: 48%.

[0070] Table 7 Results of sterility tests

[0071] Note: "+" indicates the presence of bacteria, and "-" indicates the absence of bacteria.

[0072] Testing revealed that the aerobic-anaerobic bacterial culture tubes and fungal culture tubes inoculated with the spray of this invention were clear and sterile, while the positive control tubes showed bacterial growth and the negative control tubes showed no bacterial growth, meeting the sterility requirements of GB 27951-2021 "General Requirements for Skin Disinfectants".

[0073] Test Example 8: Acute Oral Toxicity Test 1. Testing basis: Section 2.3.1 of the "Disinfection Technical Specifications" (2002 edition); 2. Dosage grouping: A dosage group of 5000 mg / kg was set up, consisting of 20 KM mice, half male and half female; 3. Preparation of test sample: Weigh 5.001g of the test sample prepared in Example 2 of this invention, add pure water to 20mL, mix thoroughly to obtain a poisoning solution with a concentration of 250mg / mL; 4. Method of administration: Animals were fasted overnight before administration, but had free access to water. The test sample was administered to the animals once by gavage, with an administration volume of 0.2 mL / 10 g body weight. 5. Observation: After exposure, observe and record the animals' signs of poisoning. Perform an autopsy on animals euthanized at the end of the observation period, observing them visually. If any abnormal tissues or organs are found, conduct further histopathological examination. The observation period is 14 days. 6. LD 50 Calculation method: Calculated according to the method in "One maximum limit test" of "Disinfection Technical Specifications" (2002 edition) 2.3.1.5 2.

[0074] Table 8 Results of Acute Oral Toxicity Test on KM Mice

[0075] After detection, no obvious abnormal symptoms and signs were found in the animals after exposure to the toxin. No other abnormal manifestations or deaths occurred during the continuous observation for 14 days. At the end of the observation period, the animals were sacrificed for autopsy. After macroscopic observation, no abnormalities were found in the main organs. The spray of the present invention was subjected to an acute oral toxicity test on KM mice, and the LD 50 > 5000 mg / kg body weight, and the toxicity classification belongs to practically non-toxic. It meets the requirements of the "Disinfection Technical Specification" (2002 edition).

[0076] Test Example 9: Single-dose Skin Irritation Test 1. Test animals: 7 Japanese white rabbits, female, weighing 2.0 kg - 2.5 kg, provided by Changyang Xishan Farm in Beijing, ordinary-grade animals, production license number SCXK (Beijing) 2021·0008, quality certificate number 110329251100054328. The feed was provided by Spbio (Beijing) Biotechnology Co., Ltd., production license number scxK (Beijing) 2024·0001, quality certificate number H03242500175040. The temperature in the animal house was 19°C - 26°C, and the relative humidity was 40% - 70%. Lighting was for 12 hours and darkness for 12 hours. The animals were allowed to feed and drink freely; 2. Detection basis: Item 2.3.3.3.2 of the "Disinfection Technical Specification" (2002 edition); 3. Preparation of test samples: Examples 1, 2, and 3, numbered 1001, 1002, and 1003 respectively, Comparative Example 1 numbered 1004, Comparative Example 2 numbered 1005, Comparative Example 3 numbered 1006, and Comparative Example 4 numbered 1007. The original test samples were used directly; 4. Poisoning method: 24 hours before the test, the hair on both sides of the spine of the Japanese white rabbits was removed, with an area of about 3 cm × 3 cm. Before applying the test sample, on the depilated skin of 2.5 cm × 2.5 cm, the exposed skin was cleaned and disinfected with 75% alcohol. After the alcohol evaporated, a "well" - shaped wound was made on the depilated skin area with a syringe needle without damaging the dermis, and 0.5 mL of the original test sample was directly dropped into the damaged skin area. Then, it was covered with a layer of non - irritating plastic film and fixed with non - irritating adhesive tape. The depilated skin on the other side was used as a blank control. The application time was 4 hours. After the test ended, the residual test sample was removed with warm water; 5. Observation and evaluation: Observe the local skin reactions and score them at 1 h, 24 h, and 48 h after removing the residual test samples. The scoring criteria and the classification of the irritation intensity refer to Tables 2-11 and 2-12 in "Disinfection Technical Specifications" (2002 Edition), Section 2.3.3, "Skin Irritation Test".

[0077] Table 9 Evaluation Results of the Single Damaged Skin Irritation Reaction of Japanese White Ears Rabbits .

[0078] After detection, observe the local skin reactions at 1 h, 24 h, and 48 h after removing the residual test samples. No skin irritation reactions were found on the poisoned sides and the control sides of the three animals of the present invention. The original liquid of the spray of the present invention was used for the single damaged skin irritation test of Japanese white ears rabbits, and the highest skin irritation index was 0.00 (0 - <0.5), and the irritation intensity was non-irritating. It meets the requirements of "Disinfection Technical Specifications" (2002 Edition). Skin irritation reactions were found on the poisoned sides of the animals in Comparative Example 2.

[0079] Test Example 10: Multiple Intact Skin Irritation Test 1. Animals: 7 Japanese white ears rabbits, female, weighing 2.0 kg - 2.5 kg, provided by Changyang Xishan Farm in Beijing, ordinary-grade animals, production license number SCXK (Beijing) 2021 - 0008, quality certificate number 110329251100054328. The feed is provided by Spbio (Beijing) Biotechnology Co., Ltd., production license number scxK (Beijing) 2024 - 0001, quality certificate number 1103242500175040. The temperature in the animal house is 19°C - 26°C, and the relative humidity is 40% - 70%. 12 h of lighting and 12 h of darkness. The animals are allowed to eat and drink freely; 2. Detection basis: Item 2.3.3.3.3 of "Disinfection Technical Specifications" (2002 Edition); 3. Preparation of test samples: For Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, directly use the original liquid of the test samples; 4. Poisoning method: 24 h before the test, depilate the areas on both sides of the spine on the back of the Japanese white ears rabbits, with an area of about 3 cm × 3 cm. The next day, apply 0.5 mL of the original liquid of the test sample on the left side of the skin, and the right side is used as a blank control. After 4 h of application, wash it with warm water to remove the residual test samples. Apply it once a day, with the same method as described above, and apply it continuously for 14 d. For the convenience of applying the test samples and observing the results, depilate again if necessary; 5. Observation and evaluation: Observe the skin reactions and score them 24 h after each application. The scoring results are shown in Tables 10 - 14 below.

[0080] Table 10 Results of repeated complete skin stimulation responses in Japanese White rabbits in Examples 1-3 .

[0081] Table 11 Results of repeated complete skin stimulation responses in Comparative Example 1 Japanese White Rabbits .

[0082] Table 12. Comparative Example 2: Scoring results of repeated complete skin stimulation responses in Japanese white rabbits. .

[0083] Table 13. Results of repeated complete skin stimulation responses in Comparative Example 3: Japanese White Rabbits. .

[0084] Table 14. Results of repeated complete skin stimulation responses in Comparative Example 4: Japanese White Rabbits. .

[0085] Testing was conducted on seven animals using multiple skin irritation tests, with local skin reactions observed 24 hours after each application. No irritation was observed on the exposed side or control side of the three animals using the spray of this invention at 14 days. Multiple complete skin irritation tests were performed on Japanese White rabbits using the undiluted spray of this invention, with a total average score, i.e., an irritation index, of 0.00 (0-<0.5), indicating non-irritating intensity. This meets the requirements of the "Disinfection Technical Specifications" (2002 edition). Animals in control example 2 showed irritation on the exposed side at 14 days.

[0086] Experimental Example 11: Treatment of a rat model of burns SD rats weighing 200-250g were randomly divided into 7 groups of 6 rats each (half male and half female). After hair removal from their backs, the rats were anesthetized and immersed in a 90℃ water bath for 15 seconds to create a 10% area third-degree burn model. Within 20 minutes of the burn, the drugs prepared in Examples 1-3 and Comparative Examples 1-4 were sprayed. The burned SD rats were housed individually and disinfected three times a day. The bedding was sterilized by high temperature and high pressure and changed daily.

[0087] Table 15. Therapeutic effects on SD rat burn model .

[0088] Conclusion: The three groups of SD rats treated with the spray of the present invention showed significantly better therapeutic effects than the control groups of SD rats. The average treatment time was 1-3 days, and the wound healing time was also significantly shortened. This indicates that the spray of the present invention quickly stops bleeding, forms a protective film, prevents fluid exudation, inhibits bacterial invasion, and promotes rapid wound healing without leaving scars.

[0089] This invention's spray formulation is a traditional Chinese medicine preparation, economical, effective, and easy to use. While ensuring efficacy, it is non-irritating to the skin and also shows significant effect on bleeding from accidental abrasions and scrapes. This invention treats burns using a "fully open therapy," allowing for timely cleaning and appropriate treatment of the wound, quickly relieving local pain. The sprayed solution immediately forms a "protective film" on the wound, effectively controlling bleeding and fluid leakage, while preventing bacterial invasion, which is crucial for preventing infection. For superficial second-degree burns, it can quickly control fluid leakage; for deep second- to third-degree burns, combined with internal administration of traditional Chinese medicine based on individual constitution, it enhances disease resistance and promotes healing, while also effectively controlling local infection and sepsis.

[0090] The sequence numbers of the above embodiments of the present invention are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments.

[0091] The embodiments of the present invention have been described above, but the present invention is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of the present invention without departing from the spirit and scope of the claims, and these forms are all within the protection scope of the present invention.

Claims

1. A burn spray, characterized in that, The ingredients, by weight, include: 4-10 parts of Typha pollen, 2-6 parts of Cirsium japonicum, 1-5 parts of Callicarpa nudiflora, 1-5 parts of Euphorbia humifusa, 3-8 parts of Verbena officinalis, 2-8 parts of Cirsium japonicum, 1-4 parts of Imperata cylindrica root, 1-10 parts of palm charcoal extract, 0.5-4 parts of bisabolol, 1-5 parts of azone, 0.5-2 parts of Leonurus japonicus extract, 0.1-1 parts of Centella asiatica extract, and 0.1-5 parts of Hypericum perforatum water extract.

2. The burn spray as described in claim 1, characterized in that: It also includes 3-5 parts borneol, 4-8 parts glycerin and 10-40 parts 70% alcohol by weight.

3. The method for preparing a burn spray as described in claim 1 or 2, characterized in that: Remove impurities from small thistle, naked-flowered purple beauty, euphorbia humifusa, verbena, tiger's beard grass, and white grass root, wash, dry, and crush them. Wash and dry cattail pollen. Soak the crushed cattail pollen, small thistle, naked-flowered purple beauty, euphorbia humifusa, verbena, tiger's beard grass, and white grass root in alcohol. Filter the liquid, add borneol to the liquid, then add glycerin, azone, plant extracts, bisabolol, and natural astringents. Dilute the alcohol concentration in the liquid with sterile distilled water. Dispense and seal.

4. The method for preparing a burn spray as described in claim 3, characterized in that: Soak the following ingredients in alcohol for 10-15 days: crushed cattail pollen, crushed small thistle, crushed naked flower purple beauty, crushed ground euphorbia, crushed verbena, crushed tiger beard grass, and crushed white grass root. Dilute the alcohol concentration in the solution with sterile distilled water to 20-30%.

5. The method for preparing a burn spray as described in claim 3, characterized in that: The method for cleaning, washing, drying, and pulverizing *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Heliotropium indicum*, and *Imperata cylindrica* includes: removing impurities from *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Heliotropium indicum*, and *Imperata cylindrica*, soaking them in a washing and moistening pool with water 3-5 cm above the herbs for 3-5 hours, then allowing them to remain moist for another 3-5 hours. Afterward, cut the herbs into small, uniform pieces or slices; dry them in an oven at 40-60 degrees Celsius with a conveyor belt speed of 0.3-0.5 meters per minute; after drying, cool to room temperature and pulverize using a pulverizer to obtain pulverized *Cirsium japonicum*, *Callicarpa nudiflora*, *Euphorbia humifusa*, *Verbena officinalis*, *Heliotropium indicum*, and *Imperata cylindrica*.

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