A dual-labeled primer system for nucleic acid library source quality control and uses thereof
By using a dual-label primer system targeting the PCARE gene and the IFT140 gene transcript variant X25, the problem of sequence origin differentiation in DNA/RNA co-construction libraries was solved, achieving high-accuracy quality control, reducing costs and improving detection efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HANGZHOU MATRIDX BIOTECH CO LTD
- Filing Date
- 2026-02-10
- Publication Date
- 2026-06-05
AI Technical Summary
Existing DNA/RNA co-construction library technologies cannot effectively distinguish the source of sequences, leading to errors in pathogen identification, quantification distortion, and problems such as species interference, sequencing resource consumption, poor degradation tolerance, and low economic efficiency in exogenous quality control schemes.
Using a first primer pair targeting the PCARE gene and a second primer pair targeting the IFT140 gene transcript variant X25, nucleic acid fragments derived from DNA and RNA were specifically amplified, respectively. By detecting the depth ratio of the amplicon in high-throughput sequencing, qualitative discrimination and quantitative monitoring of DNA and RNA-derived nucleic acid fragments were achieved.
No exogenous sequences need to be added, thus avoiding species interference, reducing costs, improving detection accuracy, ensuring the effective use of sequencing resources, and accurately reflecting the initial state of the sample, eliminating quantitative distortion.
Smart Images
Figure CN121674543B_ABST