Acrossocheilus fasciatus oviposition inducing device and oviposition collecting method

Abstract

The invention relates to the technical field of acrossocheilus fasciatus breeding, in particular to an acrossocheilus fasciatus oviposition inducing device and an oviposition collecting method. Comprising an inducing unit, a collecting unit, a mixing unit and a hatching unit which are sequentially arranged. The induction unit comprises a plurality of induction water tanks which are arranged in parallel, and a sand frame and a lifting gate are arranged in each induction water tank; the sand frames are detachably arranged in the induction water tanks, coarse sand layers are laid at the bottoms in the sand frames, lifting mechanisms are further arranged at the bottoms of the sand frames and used for lifting the sand frames, and the lifting gates of the induction water tanks are independently controlled. The oviposition inducing device is matched with a special oviposition collecting method, so that the problems of frequent trawl in artificial insemination and difficulty in batch obtaining of fertilized oviposition in non-synchronous gonad development of parent fishes can be effectively solved.

Description

Technical Field

[0001] This invention relates to the field of breeding technology for bryophytes, specifically to a device for inducing spawning and a method for collecting eggs in bryophytes. Background Technology

[0002] The broodstock, also known as the stream rockfish, prefers to live in clear, flowing water environments such as mountain streams and pebble creeks in its natural environment. Its eggs are slightly sticky and sink to the gaps between rocks or gravel to hatch. Due to its tender, delicious, and nutritious flesh, the broodstock is a small economic fish with high economic value and can be developed into an industrialized species. In the artificial breeding of broodstock, obtaining a large number of high-quality fertilized eggs has always been a technical problem that has hindered the development of the broodstock aquaculture industry. In early research and practice, people tried to obtain fertilized eggs by artificially inducing spawning, but found that no matter how the spawning hormones were selected and prepared, the parent fish were not sensitive to the hormones. Later, it was found that broodstock prefer to lay their eggs in coarse sand, so the sand frame induction method was tried to obtain fertilized eggs.

[0003] However, traditional sand frame induction methods require manual removal or sifting of fertilized eggs from the sand, which causes significant damage to the fertilized eggs of the Gynostemma pentaphyllum. Furthermore, coarse sand can cause some fertilized eggs to mix with the sand, making complete separation difficult and hindering the acquisition of fertilized eggs in bulk. Therefore, there is an urgent need for a device and method to induce spawning in Gynostemma pentaphyllum. Research and practice have shown that only broodstock with mature gonads will enter the sand frame. The traditional sand frame can be used as a tool to select spawning broodstock. Once the broodstock are in the frame, they can be captured and artificially inseminated. This avoids the problem of difficulty in obtaining fertilized eggs in bulk in traditional egg collection methods and solves the problem of frequent trawls during artificial insemination caused by the asynchronous development of gonads in broodstock. Summary of the Invention

[0004] The purpose of this invention is to solve the problems in the background art by providing a device for inducing spawning and a method for collecting eggs in the broodstock, which can effectively solve the problems of frequent trawling for artificial insemination and difficulty in obtaining fertilized eggs in batches when the gonads of parent fish are not developing synchronously.

[0005] The above-mentioned technical objective of the present invention is achieved through the following technical solution: A device for inducing spawning in a glossy bream includes an induction unit, a collection unit, a mixing unit, and an incubation unit arranged sequentially. The induction unit includes multiple induction tanks arranged in parallel, each of which is equipped with a sand frame and a lifting gate. The sand frame is detachably installed in the induction tank and has a coarse sand layer laid at the bottom. Each sand frame is also equipped with a lifting mechanism at the bottom for lifting the sand frame. The lifting gate of each induction tank is independently controlled.

[0006] Preferably, a transparent partition is provided between the multiple induction tanks for separation. The upper part of the transparent partition is open and the lower part is solid. Each induction tank is also equipped with a sensor for monitoring the behavior of parent fish entering the frame.

[0007] Preferably, the lifting mechanism includes a main body, a lifting rod, and a lifting platform. The main body includes a fixed guide post, on which a movable guide sleeve is movably disposed. The lifting platform is disposed at the end facing away from the main body and is fixedly connected to the movable guide sleeve. The movable guide sleeve is used to restrict the movement of the lifting platform along the direction of the fixed guide post.

[0008] Preferably, a hinged column perpendicular to the fixed guide column is fixedly installed at the bottom of the main body of the mechanism. A mounting part is fixedly installed on the lifting rod, and the lifting rod is hinged to the hinged column through the mounting part. A lifting connecting part is provided at the end of the lifting rod near the lifting platform. A first connecting rod and a second connecting rod are also provided at the bottom of the lifting platform, which are hinged to each other. The first connecting rod is hinged to the lifting platform, and the second connecting rod is hinged to the lifting connecting part.

[0009] Preferably, a lifting block is fixedly installed on the platform of the lifting platform, and a pressing block is installed on the end of the lifting rod away from the lifting platform. The sand frame is detachably installed on the lifting platform, and the position of the pressing block corresponds to the position of the lifting gate.

[0010] Preferably, the collection unit includes an adjustable fixing frame for fixing the parent fish and a negative pressure suction device. The negative pressure suction device includes a suction head, a peristaltic pump and a collection container, which is placed in a collection box with heat preservation function.

[0011] Preferably, the mixing unit includes a stirring container and a low-speed stirring mechanism. The stirring container has a feed inlet, which is connected to the collection container containing semen and the collection container containing eggs via pipelines.

[0012] Preferably, the side wall of the stirring container is provided with a liquid inlet for adding physiological saline or sperm activation solution, and the stirring paddle of the low-speed stirring mechanism is made of flexible material with a rotation speed of 5-20 revolutions per minute.

[0013] Preferably, the incubation unit includes tiered incubation tanks, each of which is equipped with a microporous incubation bed for holding fertilized eggs, and a spray head is also provided above the tank.

[0014] A device for inducing spawning and a method for collecting eggs in a glossy bream, characterized by comprising the following steps: Inducing broodstock: Select healthy, disease-free, and mid-stage gonadal-developing broodstock of the *Scleroderma scoparia* and place them in the induction unit at a female-to-male ratio of 2:1. After intensive cultivation to ensure good gonadal development, use the environment of the induction unit to induce them to enter the sand frame. After identification by sensors, control the lifting gate to fall and isolate the target broodstock. Sperm and eggs were collected. The isolated parent fish were manually caught and fixed on an adjustable support frame. Mature parent fish were wrapped with sterile towels and their abdomens were gently pressed. Sperm and eggs were collected separately using a negative pressure suction device and temporarily stored in collection containers. For artificial insemination, the collected eggs are introduced into the mixing unit's mixing container, and then the collected semen is introduced into the mixing unit's mixing container. At the same time, the low-speed mixing mechanism is turned on to ensure that the sperm and eggs are thoroughly mixed. The amount of semen used is until the sperm and egg fluid is light off-white. Sperm activation: After the sperm and eggs are thoroughly mixed, add a small amount of water to the mixing container through the liquid inlet to activate the sperm. At the same time, turn on the low-speed stirring mechanism to stir the sperm and eggs for 20 to 30 seconds. After standing for 2 to 3 minutes, wash the fertilized eggs with sterile water and wait for incubation. For incubation, transfer the fertilized eggs to the microporous incubation bed in the incubation tank, turn on the spray head for micro-flow incubation, maintain the water temperature at 21℃-24℃, pH value at 6.8-7.6, and dissolved oxygen at 6mg / L or above, and clean the attached substrate and dead or moldy eggs daily; after the fertilized eggs break through the membrane, increase the water flow of the spray head appropriately, and after the fry swim horizontally, transfer them to the rearing pond.

[0015] In summary, the beneficial effects of this invention are as follows: 1. The present invention provides a device and method for inducing spawning of *Scleroderma scoparia*, which, by setting up an induction unit, utilizes the sand-loving habit of *Scleroderma scoparia* and uses a biomimetic sand frame to naturally induce the maturation of gonads in parent fish, thereby achieving precise selection of parent fish and effectively solving the problem of asynchronous gonad development in parent fish during traditional aquaculture. 2. The spawning induction device and egg collection method for the broodstock described in this invention includes a lifting mechanism located below the induction unit. When the broodstock enters and stays in the sand frame, the lifting gate of the induction unit automatically closes, and at the same time, the lifting mechanism is activated to raise the position of the sand frame. This allows the aquaculture personnel to more intuitively observe which sand frame has been entered by the broodstock. This achieves the process of automatic lifting of the sand frame after the broodstock enters the sand frame, and also avoids the problem of frequent trawling required for broodstock harvesting in traditional aquaculture. 3. The spawning induction device and egg collection method for the bryophyll fish described in this invention collects fresh sperm and eggs separately through a negative pressure device and immediately mixes and stirs them under controllable conditions. The entire process of collecting sperm and eggs is gentle, and the fertilization process is carried out in a special container, which completely avoids the physical damage to the eggs caused by traditional sand frame sieving, while ensuring the activity and contact efficiency of sperm and eggs, and realizing the need to obtain fertilized eggs in batches. 4. The spawning induction device and egg collection method for the bryophyll carp described in this invention simulates the natural attachment environment and flowing water conditions in the incubation unit. Combined with the control of the incubation environment conditions in the incubation unit, the accumulation of fertilized eggs and lack of oxygen are effectively avoided, thereby improving the hatching rate and increasing the efficiency of aquaculture production. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the induction unit structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the induction unit of the present invention; Figure 3 This is a schematic diagram of the lifting mechanism structure of the present invention; Figure 4 This is a schematic diagram of the collection unit structure of the present invention; Figure 5 This is a schematic diagram of the hybrid unit structure of the present invention; Figure 6 This is a schematic diagram of the incubation unit structure of the present invention.

[0017] The diagram shows: 1. Induction unit; 11. Induction tank; 12. Sand frame; 13. Lifting gate; 14. Coarse sand layer; 15. Transparent partition; 16. Sensor; 2. Collection unit; 21. Fixing frame; 22. Negative pressure suction device; 221. Suction head; 222. Peristaltic pump; 223. Collection container; 224. Collection box; 3. Mixing unit; 31. Stirring container; 311. Feed inlet; 312. Liquid inlet; 32. Low speed 33. Stirring mechanism; 4. Holding dish; 5. Incubation unit; 6. Incubation tank; 7. Microporous incubation bed; 8. Spray head; 9. Lifting mechanism; 10. Main body of the mechanism; 11. Fixed guide column; 12. Movable guide sleeve; 13. Hinge column; 14. Lifting rod; 15. Mounting part; 16. Lifting connection part; 17. Pressing block; 18. Lifting platform; 19. First connecting rod; 10. Second connecting rod; 11. Lifting block. Detailed Implementation

[0018] The following specific embodiments are merely illustrative of the present invention and are not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to these embodiments without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of the present invention.

[0019] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0020] Example

[0021] according to Figures 1-6As shown, a device for inducing spawning of a bryophyte includes an induction unit 1, a collection unit 2, a mixing unit 3, and a hatching unit 4 arranged in sequence. The induction unit 1 includes multiple induction tanks 11 arranged in parallel. Each induction tank 11 is equipped with a sand frame 12 and a lifting gate 13. The sand frame 12 is detachably installed in the induction tank 11 and a coarse sand layer 14 is laid at the bottom of the frame. Each sand frame 12 is also equipped with a lifting mechanism 5 at the bottom for lifting the sand frame 12. The lifting gate 13 of each induction tank 11 is independently controlled.

[0022] according to Figure 1 , Figure 2 As shown, in this embodiment, a transparent partition 15 is provided between multiple induction tanks 11 for separation. The upper part of the transparent partition 15 is open and the lower part is closed. Each induction tank 11 is also provided with a sensor 16 for monitoring the behavior of parent fish entering the frame.

[0023] The induction unit 1 has multiple induction tanks 11 arranged parallel to the water flow direction. Each channel is separated by a transparent partition 15, forming a transparent tank with an open upper part and a closed lower part. This reduces mutual interference between parent fish in different channels, facilitates observation of the parent fish's condition, and does not affect the overall water circulation. Each induction channel is equipped with a sand frame 12, which has a mesh structure at the bottom and a coarse sand layer 14 inside, simulating a sandy environment by utilizing the parent fish's preference for sand. A sensor 16 is installed at the entrance of each sand frame 12. When a parent fish is detected entering the frame and staying for more than a preset time, the sensor 16 transmits a signal to determine that the parent fish is a mature parent fish to be operated. At this time, the lifting gate 13 in the induction tank 11 will isolate the target parent fish in the sand frame 12 area of ​​that induction tank 11, while other channels remain open. The preset time can be adjusted according to the actual state of the parent fish released. This design realizes automated screening with immediate isolation upon detection and can process multiple parent fish simultaneously, improving screening efficiency.

[0024] according to Figure 2 , Figure 3As shown, in this embodiment, the lifting mechanism 5 includes a mechanism body 51, a lifting rod 52, and a lifting platform 53. The mechanism body 51 includes a fixed guide post 511, on which a movable guide sleeve 512 is movably disposed. The lifting platform 53 is disposed at the end facing away from the mechanism body 51 and is fixedly connected to the movable guide sleeve 512. The movable guide sleeve 512 is used to restrict the movement of the lifting platform 53 along the direction of the fixed guide post 511. A hinge post 513 perpendicular to the fixed guide post 511 is fixedly disposed at the bottom position inside the mechanism body 51. A mounting part 521 is fixedly disposed on the lifting rod 52, and the lifting rod 52 passes through the mounting part 521. Hinged to the hinge column 513, the lifting rod 52 has a lifting connection part 522 at one end near the lifting platform 53. The bottom of the lifting platform 53 also has a first connecting rod 531 and a second connecting rod 532 that are hinged to each other. The first connecting rod 531 is hinged to the lifting platform 53, and the second connecting rod 532 is hinged to the lifting connection part 522. A lifting block 533 is fixedly installed on the platform of the lifting platform 53. A pressing block 523 is installed at the end of the lifting rod 52 away from the lifting platform 53. The sand frame 12 is detachably installed on the lifting platform 53. The position of the pressing block 523 corresponds to the position of the lifting gate 13.

[0025] The sand frame 12 is installed on the lifting platform 53 of the lifting mechanism 5. When the parent fish are detected entering the area of ​​the sand frame 12, the lifting gate 13 on the top of the pressing block 523 will automatically lower. When the lifting gate 13 continues to press down and contacts the pressing block 523, the lifting rod 52 at the bottom of the pressing block 523 will move downward under the pressure of the lifting gate 13. Since the lifting rod 52 is hinged to the fixed hinge column 513, the hinge column 513 will act as a fulcrum to turn the lifting rod 52 into a lever structure. The pressing block 523 at one end of the lifting rod 52 will drive the lifting platform 53 at the other end to rise. Since the end of the lifting rod 52 is connected to the lifting platform 53 through the first connecting rod 531 and the second connecting rod 532, and the lifting platform 53 is fixed on the movable guide sleeve 512, the lifting platform 53 will rise vertically along the direction of the fixed guide column 511. A lifting block 533 is fixedly installed on the lifting platform 53. The vertical rise of the lifting block 533 can drive the sand frame 12 fixed on it to move upward together. At this time, the sand frame 12 will be raised to a position higher than the induction tank 11. The operator can directly observe which sand frame 12 in the induction tank 11 has entered the parent fish. The parent fish in the sand frame 12 after being raised can be caught, avoiding the problem of frequent trawls required in traditional aquaculture. After the parent fish in the sand frame 12 are caught, the lifting gate 13 only needs to be raised. At this time, the sand frame 12 will automatically descend into the induction tank 11 under the action of gravity to continue to induce the parent fish. The lifting mechanism 5 and the lifting gate 13 realize the linkage of the lifting gate 13 descending and automatically raising the sand frame 12, reducing the need for the operator to frequently observe whether the sand frame 12 in the water has entered the parent fish, making the operation of the device more intuitive.

[0026] according to Figure 4 As shown, in this embodiment, the collection unit 2 includes a fixing frame 21 for fixing the parent fish and a negative pressure suction device 22. The negative pressure suction device 22 includes a suction head 221, a peristaltic pump 222 and a collection container 223. The collection container 223 is disposed in a collection box 224 with heat preservation function.

[0027] The fixing frame 21 for securing the parent fish is adjustable to accommodate parent fish of different sizes. The parent fish are held and secured gently using a soft, sterilized towel in conjunction with the fixing frame 21 to reduce stress on the parent fish. At the same time, two independent negative pressure suction devices 22 are configured, one for collecting semen and the other for collecting eggs. The two systems are independent to avoid cross-infection. The collection container 223 is placed in the collection box 224. The temperature inside the collection box 224 is maintained at the temperature that ensures the maximum activity of sperm and eggs to maximize their viability.

[0028] according to Figure 5As shown, in this embodiment, the mixing unit 3 includes a stirring container 31 and a low-speed stirring mechanism 32. The stirring container 31 has a feed inlet 311, which is connected to a collection container 223 containing semen and a collection container 223 containing eggs through pipelines. The stirring container 31 has a liquid inlet 312 on its side wall for adding physiological saline or sperm activation solution. The stirring paddle of the low-speed stirring mechanism 32 is made of flexible material and its rotation speed is 5-20 revolutions per minute.

[0029] The mixing container 31 is made of transparent material for easy observation. The low-speed stirring mechanism 32 inside the mixing container 31 is a flexible stirring paddle whose speed can be adjusted within a low range to ensure that the sperm and eggs are fully mixed in the holding dish 33 without damaging the cells due to excessive shearing force caused by the stirring paddle material being too hard or the speed being too fast. The mixing container 31 is equipped with a liquid inlet 312 for adding a small amount of water or a special sperm activation solution during the mixing process to start the fertilization process.

[0030] according to Figure 6 As shown, in this embodiment, the incubation unit 4 includes a tiered incubation tank 41, each of which is provided with a microporous incubation bed 42 for carrying fertilized eggs, and a spray head 43 is provided above the tank body of the incubation tank 41.

[0031] The incubation unit 4 adopts a multi-layered stepped trough-type flowing water incubation design. Each incubation trough 41 is equipped with a microporous incubation bed 42, such as a nylon mesh. Since the fertilized eggs of the bryophyll carp have a certain degree of stickiness, the mesh on the microporous incubation bed 42 is used to support and fix the sticky fertilized eggs. A spray head 43 is installed above the trough. The spray head 43 is connected to an air pump and a water supply pipe to provide dissolved oxygen water flow. The stepped incubation trough 41 and spray-type flowing water design can simulate the stream environment in the natural environment, providing the most suitable incubation environment for the fertilized eggs. The spray head 43 above the incubation trough 41 can provide sufficient dissolved oxygen and form a micro-water flow environment, which can effectively prevent the eggs from accumulating, lacking oxygen and becoming moldy, thus improving the incubation efficiency of the fertilized eggs.

[0032] In this embodiment, a device for inducing spawning and a method for collecting eggs in a glossy bream include the following steps: Inducing broodstock: Select healthy, disease-free, and mid-stage gonadal-developing broodstock and place them in induction unit 1 at a male-to-female ratio of 2:1. After enhanced cultivation to ensure good gonadal development, use the environment of induction unit 1 to induce them to enter sand frame 12. After identification by sensor 16, control the lifting gate 13 to fall and isolate the target broodstock. Collect sperm and eggs. After the isolated parent fish are manually caught, they are fixed on the adjustable fixing frame 21. The mature parent fish are wrapped with a sterile towel, and the abdomen of the parent fish is gently pressed. The sperm and eggs are collected separately using the negative pressure suction device 22 and temporarily stored in the collection container 223. Artificial insemination involves introducing the collected eggs into the mixing container 31 of the mixing unit 3, followed by introducing the collected semen into the mixing container 31 of the mixing unit 3, while simultaneously turning on the low-speed stirring mechanism 32 to thoroughly mix the sperm and eggs. The amount of semen used should be such that the sperm and egg fluid is light off-white. After sperm activation and thorough mixing of sperm and eggs, a small amount of water is added to the mixing container 31 through the liquid inlet 312 to activate the sperm. At the same time, the low-speed stirring mechanism 32 is turned on to stir the sperm and eggs for 20 to 30 seconds. After standing for 2 to 3 minutes, the fertilized eggs are washed with sterile water and placed for incubation. For incubation, transfer the fertilized eggs to the microporous incubation bed 42 of the incubation tank 41, turn on the spray head 43 for micro-flow incubation, maintain the water temperature at 21℃-24℃, pH value at 6.8-7.6, and dissolved oxygen at 6mg / L or above, and clean the attached substrate and dead or moldy eggs daily; after the fertilized eggs break through the membrane, appropriately increase the water flow of the spray head 43, and after the fry swim horizontally, transfer them to the rearing pond.

Claims

1. A device for inducing spawning in *Gymnocypris spp.*, characterized in that, The system includes an induction unit (1), a collection unit (2), a mixing unit (3), and an incubation unit (4) arranged in sequence. The induction unit (1) includes multiple induction tanks (11) arranged in parallel. Each induction tank (11) is equipped with a sand frame (12) and a lifting gate (13). The sand frame (12) is detachably installed in the induction tank (11) and a coarse sand layer (14) is laid at the bottom of the frame. Each sand frame (12) is also equipped with a lifting mechanism (5) at the bottom for lifting the sand frame (12). The lifting gate (13) of each induction tank (11) is independently controlled.

2. The device for inducing spawning in *Gymnocypris spp.* according to claim 1, characterized in that, A transparent partition (15) is provided between the multiple induction tanks (11) for separation. The transparent partition (15) is open at the top and closed at the bottom. Each induction tank (11) is also provided with a sensor (16) for monitoring the behavior of parent fish entering the frame.

3. The device for inducing spawning in *Gymnocypris spp.* according to claim 1, characterized in that, The lifting mechanism (5) includes a main body (51), a lifting rod (52), and a lifting platform (53). The main body (51) includes a fixed guide post (511), and a movable guide sleeve (512) is movably provided on the fixed guide post (511). The lifting platform (53) is located at one end away from the main body (51) and is fixedly connected to the movable guide sleeve (512). The movable guide sleeve (512) is used to restrict the lifting platform (53) from moving along the direction of the fixed guide post (511).

4. The device for inducing spawning in *Gymnocypris spp.* according to claim 3, characterized in that, A hinge column (513) perpendicular to the fixed guide column (511) is fixedly installed at the bottom position of the main body (51) of the mechanism. An installation part (521) is fixedly installed on the lifting rod (52). The lifting rod (52) is hinged to the hinge column (513) through the installation part (521). A lifting connection part (522) is provided at the end of the lifting rod (52) near the lifting platform (53). A first connecting rod (531) and a second connecting rod (532) are also provided at the bottom position of the lifting platform (53). The first connecting rod (531) is hinged to the lifting platform (53), and the second connecting rod (532) is hinged to the lifting connection part (522).

5. The device for inducing spawning in *Scleroderma scoparia* according to claim 4, characterized in that, A lifting block (533) is fixedly installed on the platform (53). A pressing block (523) is installed on the end of the lifting rod (52) away from the lifting platform (53). The sand frame (12) is detachably installed on the lifting platform (53). The position of the pressing block (523) corresponds to the position of the lifting gate (13).

6. The device for inducing spawning in *Gymnocypris spp.* according to claim 1, characterized in that, The collection unit (2) includes a fixing frame (21) for fixing the parent fish and a negative pressure suction device (22). The negative pressure suction device (22) includes a suction head (221), a peristaltic pump (222) and a collection container (223). The collection container (223) is placed in a collection box (224) with heat preservation function.

7. The device for inducing spawning in *Gymnocypris spp.* according to claim 6, characterized in that, The mixing unit (3) includes a stirring container (31) and a low-speed stirring mechanism (32). The stirring container (31) is provided with a feed inlet (311), which is connected to the collection container (223) containing semen and the collection container (223) containing eggs through pipelines.

8. The device for inducing spawning in *Scleroderma scoparia* according to claim 7, characterized in that, The stirring container (31) has a liquid inlet (312) on its side wall for adding physiological saline or sperm activation solution. The stirring paddle of the low-speed stirring mechanism (32) is made of flexible material and its rotation speed is 5-20 revolutions per minute.

9. The device for inducing spawning in *Gymnocypris spp.* according to claim 1, characterized in that, The incubation unit (4) includes a tiered incubation tank (41), each of which is equipped with a microporous incubation bed (42) for carrying fertilized eggs, and a spray head (43) is provided above the tank body of the incubation tank (41).

10. A device for inducing spawning and a method for collecting eggs in *Sclerodermus fasciatus* according to claims 1-9, characterized in that, Includes the following steps: S1. Inducing parent fish: Select healthy, disease-free, and mid-stage gonadal development broodstock of the broodstock and place them in the induction unit (1) at a male-to-female ratio of 2:

1. After enhanced cultivation, their gonads develop well. Using the environment of the induction unit (1), they are induced to enter the sand frame (12). After identification by the sensor (16), the lifting gate (13) is controlled to fall to isolate the target parent fish. S2. Collect sperm and eggs. After the isolated parent fish are manually caught, they are fixed on an adjustable frame (21). The mature parent fish are wrapped with a sterile towel and the abdomen of the parent fish is gently pressed. The sperm and eggs are collected separately using a negative pressure suction device (22) and temporarily stored in a collection container (223). S3. Artificial insemination: The collected eggs are introduced into the mixing container (31) of the mixing unit (3), and then the collected semen is introduced into the mixing container (31) of the mixing unit (3). At the same time, the low-speed stirring mechanism (32) is turned on to mix the sperm and eggs thoroughly. The amount of semen used is until the sperm and egg fluid is light off-white. S4. Sperm activation: After the sperm and eggs are fully mixed, add a small amount of water to the stirring container (31) through the liquid inlet (312) to activate the sperm. At the same time, turn on the low-speed stirring mechanism (32) to stir the sperm and eggs for 20 to 30 seconds. After standing for 2 to 3 minutes, wash the fertilized eggs with sterile water and wait for incubation. S5. Hatching: Transfer the fertilized eggs to the microporous hatching bed (42) of the hatching tank (41), turn on the spray head (43) for micro-flow hatching, maintain the water temperature at 21℃-24℃, pH value at 6.8-7.6, and dissolved oxygen at 6mg / L or above, and clean the attached substrate and dead and moldy eggs daily; after the fertilized eggs break through the membrane, increase the water flow of the spray head (43) appropriately, and transfer the fry to the rearing pond after they swim horizontally.

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