Method for high-temperature efficient maturation of warm water nature of shellfish gonad

Abstract

This invention provides a method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods. Addressing the problem of low gonad maturity in warm-water shellfish spawn during high-temperature periods, this method utilizes the principle that the feeding, growth, and development of poikilothermic animals are positively correlated with environmental water temperature. Based on the biological characteristics of animals exhibiting compensatory feeding and development, the method promotes rapid gonad development in spawning shellfish through alternating alternating temperature culture (6 hours of low-temperature fasting followed by 18 hours of high-temperature feeding culture daily, repeated in cycles). In just two weeks, over 80% of the scallop gonads can develop from the vacuolar stage to maturity, while only 20% of spawning shellfish in natural sea areas reach maturity. This invention effectively solves the problem of low gonad maturity in the production of warm-water shellfish seedlings during high-temperature periods. Therefore, the method of this invention has the advantages of high efficiency, short processing time, and strong applicability.

Description

Technical Field

[0001] This invention relates to the field of marine aquaculture, specifically to the field of shellfish aquaculture, and specifically to a method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods. Background Technology

[0002] The gonadal development of shellfish is generally divided into five stages: vacuolation, formation, proliferation, maturity, and release. Only broodstock shellfish whose gonads have reached maturity can effectively reproduce. The optimal breeding water temperature varies greatly among different shellfish species. For example, the breeding water temperature for the *Ctenopharynx scallop* is 16-22℃, for the *Sinocyclocheilus scallop* it is 8-8.5℃, for the *Sinocyclocheilus balsamina* it is 20-30℃, for the *Ctenopharynx nobilis* it is 20-28℃, and for the *Abalone simonii* it is 20-24℃. Therefore, water temperature is one of the most important environmental factors affecting the gonadal maturity of shellfish. In artificial shellfish breeding, the maturity of the gonads of broodstock shellfish is one of the most crucial aspects. The maturity of the gonads not only determines the yield of seedlings but also affects their quality. Therefore, to improve the yield and quality of shellfish seedlings, the maturity of the gonads of broodstock shellfish has always been a focus for shellfish breeding companies. Generally, the maturity (gona index) of shellfish gonads before they reach maturity is positively correlated with water temperature. Therefore, raising the temperature to improve the gonad maturity of broodstock is a common method in shellfish breeding in northern my country. This involves moving broodstock from the sea to indoor cement tanks during the colder season (winter, when the ambient water temperature is below 10°C). The temperature is then slowly increased (by 1°C every 3-4 days, gradually rising to 23-24°C), and the broodstock are fed single-celled algae. After more than a month of cultivation, over 80% of the broodstock gonads can reach maturity. However, for warm-water shellfish (i.e., those living in habitats with a minimum water temperature of 10°C), the broodstock used in breeding production have traditionally been collected from individuals in marine aquaculture populations whose gonads are at maturity, and artificial maturation of the broodstock gonads is usually unnecessary. However, in recent years, with global climate change and the development of the shellfish farming industry, problems such as low gonad maturity and asynchronous gonad development in shellfish farmed in the southern sea areas of my country during high-temperature periods have become increasingly prominent, seriously affecting the yield and quality of seedlings.

[0003] The noble scallop (Scallop 'Noble') is an important marine aquaculture species in southern my country, naturally distributed in the waters of Thailand, Vietnam, and Hainan, Guangdong, Guangxi, and southern Fujian provinces of my country. It is a typical warm-water scallop, with its breeding season generally from May to October, and a breeding water temperature of 22-28℃. Since successfully cultivating artificial seedlings in the late 1970s, for over 40 years, all noble scallop seedlings farmed in southern my country have come from indoor artificial breeding (conventional breeding). The broodstock used in seed production are generally collected from individuals in the aquaculture population at the stage of gonadal maturity, usually without the need for artificial gonadal maturation. However, in recent years, with the promotion and application of the new noble scallop variety "Nanao Golden Scallop," the noble scallop aquaculture industry has expanded, leading to a growing demand for seedlings, especially high-quality seedlings. Therefore, aquaculture enterprises and farmers require high-quality seedlings year-round. Because the noble scallop is a warm-water shellfish, its breeding season is generally from May to October. During this period, the water temperature is usually between 25-30°C, especially during the high-temperature period from June to September, when the seawater temperature in the south often exceeds 27°C. As a result, the gonadal maturity of noble scallops farmed in these areas is very low, with less than 20% of individuals reaching sexual maturity. This low gonadal maturity problem results in a shortage of broodstock for seedling production, severely impacting the yield and quality of noble scallop seedlings, thus hindering the healthy development of the aquaculture industry. Therefore, to improve the yield and quality of noble scallop seedlings and ensure the healthy development of the aquaculture industry, it is essential to develop a method that can efficiently promote the maturation of gonads in warm-water shellfish during high-temperature periods. Summary of the Invention

[0004] The purpose of this invention is to provide a method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods. This involves transferring broodstock shellfish in the gonadal vacuolation stage to indoor low-temperature seawater (15°C) during a high-temperature period (seawater temperature above 27°C) and fasting them for 6 hours daily. Then, they are fed fresh natural seawater for another 18 hours. This cycle is repeated, and after two weeks, over 80% of the broodstock shellfish gonads reach maturity and can be used for seed production, thus solving the problem of low gonadal maturity in the production of warm-water shellfish seedlings during high-temperature periods.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0006] A method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods includes: conducting alternating hot and cold temperature aquaculture on shellfish to obtain mature gonads.

[0007] The technical principle of this invention is as follows: It utilizes the principle that the feeding, growth, and development of poikilothermic animals are positively correlated with environmental water temperature. Furthermore, based on the biological characteristics of animals exhibiting compensatory feeding and development, by transferring broodstock from low-temperature, fasting rearing to a high-temperature environment and feeding them, compensatory feeding and development can be achieved, leading to rapid gonadal development. This invention can solve the problem of low gonadal maturity in the production of warm-water shellfish seedlings during high-temperature periods.

[0008] Preferably, the alternating hot and cold temperature aquaculture includes: aquaculture at low water temperature, followed by aquaculture at high water temperature, in a cyclical operation; the shellfish includes scallops.

[0009] Preferably, in each cycle, the time spent culturing at the high water temperature is twice the time spent culturing at the low water temperature; the temperature difference between the low water temperature and the high water temperature is not less than 10°C.

[0010] Preferably, in each cycle, the culture time at the low water temperature is 6 hours; the culture time at the high water temperature is 18 hours; the low water temperature is 15℃; and the high water temperature is 27~31℃.

[0011] Preferably, during each cycle of operation, the shellfish are kept from feeding while being cultured at the low water temperature; and the gonads of the shellfish are not yet fully developed.

[0012] Preferably, the shellfish includes broodstock that are in the high-temperature period and whose gonadal development is in the vacuolar stage.

[0013] A method for efficiently promoting the maturation of gonads in warm-water mollusks during high-temperature periods involves culturing broodstock with gonads in the vacuolar stage during high temperatures in indoor low-temperature seawater (15°C) for 6 hours daily without feeding, followed by culturing in fresh natural seawater (above 27°C) for 18 hours daily, repeating this cycle. After two weeks, over 80% of the broodstock's gonads have reached maturity, compared to only 20% in naturally occurring seawater. This invention effectively solves the problem of low gonad maturity in the production of warm-water mollusks during high-temperature periods. Therefore, the method of this invention has advantages such as high efficiency, short processing time, and strong applicability.

[0014] Preferably, the maturation of the gonads of broodstock is promoted during the high-temperature period, the maturation method adopts alternating temperature changes, the gonads of broodstock develop from the vacuolar stage to the mature stage in only 2 weeks, and the maturation efficiency is as high as 80% or more.

[0015] Preferably, the shellfish includes one or more of the following: noble scallop, Gulf scallop, Hepu pearl oyster, and Fujian oyster.

[0016] Preferred, including:

[0017] A. Select the shellfish and raise them in indoor natural seawater under low light conditions. During this period, continuously aerate and feed them, do not change the water or add antibiotics, and monitor the water temperature to obtain domesticated shellfish.

[0018] B. Transfer the domesticated shellfish into a low-temperature recirculating aquaculture system, and raise them at a low temperature of 15℃ for 6 hours / day without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added.

[0019] C. Then transfer them back to fresh natural seawater and feed them for 18 hours / day. During this period, continuously aerate and feed them, do not change the water or add antibiotics, and monitor the water temperature.

[0020] D. Repeat steps B and C for 2 weeks to obtain mature gonadal mollusks.

[0021] Preferred, including:

[0022] A. Select the shellfish and acclimate them in indoor natural seawater under low light for 1 day. During this period, continuously aerate the shellfish, feed them with single-celled diatoms once every 3 hours, do not change the water, do not add antibiotics, and monitor the water temperature once every 8 hours to obtain acclimatized shellfish. During the acclimatization period, sample the gonads of the breeding shellfish to observe their gonadal fullness and color, measure their gonadal index, and observe their gonadal histology.

[0023] B. The domesticated shellfish are transferred into the low-temperature recirculating aquaculture system consisting of a chiller and a 500-L fiberglass culture tank. They are kept at a low temperature of 15℃ for 6 hours / day without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added.

[0024] C. Then transfer them back to fresh natural seawater and feed them for 18 hours / day. During this period, continuously aerate the water, feed them single-celled diatoms once every 3 hours, do not change the water or add antibiotics, and monitor the water temperature once every 6 hours.

[0025] D. Repeat steps B and C for 2 weeks to obtain mature gonadal molluscs. Sampling and observation of gonadal fullness and color, measurement of gonadal index, and observation of gonadal histology are performed.

[0026] Peripheral changes in the gonads are observed visually, focusing on their color and fullness. The gonadal index is calculated by surgically removing the gonads and soft tissue from each individual, weighing them separately using an electronic balance (accuracy 0.01g), and then calculating the gonadal index: Gonadal index = Gonadal wet weight / Soft tissue wet weight × 100. Histological observation of the gonads uses standard methods: HE (hematoxylin-eosin) staining (Howard et al., 2004).

[0027] The above-mentioned method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods is applied to shellfish breeding.

[0028] Implementing this invention has the following beneficial effects:

[0029] 1. High efficiency. This invention can efficiently promote the maturation of mollusc gonads during high-temperature periods, with over 80% of the gonads reaching maturity, far exceeding the 20% in natural sea areas.

[0030] 2. Short time required. The gonads of breeding shellfish develop from the vacuolar stage to maturity in just 2 weeks, while existing methods for promoting gonad maturation in shellfish take more than a month.

[0031] 3. High applicability. This invention can effectively solve the problem of insufficient breeding stock for warm-water shellfish during high-temperature periods, and the mature breeding stock can be directly used in seedling production. Attached Figure Description

[0032] Figure 1 The above-ground and histological photographs of the testes and ovaries of the molluscs are taken using the method step (2) of this invention in Example 1.

[0033] Figure 2 The above are the epitaxial and histological photographs of the testes and ovaries of the molluscs in Example 1, which are obtained by using the method step (7) of the present invention. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to specific embodiments.

[0035] Example 1

[0036] Time: June 12, 2025 - June 28, 2025; Location: Shantou University Nan'ao Coastal Experimental Station; Natural sea temperature: 27.4±0.3℃.

[0037] On June 12, 2025, 300 healthy, undamaged, and immature male and female *Ctenopharynx nobilis* scallops were selected. The shells were cleaned of any attached debris and rinsed thoroughly with natural seawater. They were then placed indoors for 30 minutes. 3 In cement tanks, scallops were acclimatized for one day under low light conditions with natural seawater, followed by 6 hours of low-temperature, fasted rearing, and then transferred back to fresh natural seawater for 18 hours. During this period, they were continuously aerated and fed single-celled diatoms (food) once every 3 hours. This process was repeated for 14 days until 80% of the scallops reached sexual maturity. The specific steps are as follows:

[0038] 1. Selection and rearing of broodstock. Select 300 healthy, undamaged, and immature male and female *Ctenopharynx nobilis* scallops. Clean the shells of any attached organisms and wash them thoroughly with natural seawater. Place them in an indoor cement tank and rear them in natural seawater using conventional methods for one day. During this period, continuously aerate the tank, feed them single-celled diatoms once every 3 hours, do not change the water, and do not add antibiotics. Measure the water temperature once every 8 hours.

[0039] 2. Gonadal Maturity Examination: After domestication, the fullness and color of the gonads were examined. Three male and three female scallops were randomly selected, and their gonadal index was measured and their gonadal histology was observed. Taking the observation results of one male and one female scallop as an example, it was found that both the testes and ovaries were empty, transparent, and colorless. Figure 1 The gonadal indices of the testes and ovaries were 6.60%±0.08% and 8.11%±0.18%, respectively, and both gonadal tissues were in the vacuolar phase. Figure 1 ).

[0040] 3. Low-temperature fasting culture: After domestication, the breeding shellfish are transferred to a low-temperature recirculating aquaculture system consisting of a chiller and 500-L fiberglass culture tanks, with 100 shellfish per tank. They are kept at a low temperature of 15℃ for 6 hours without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added.

[0041] 4. High-temperature seawater feeding culture; after a 6-hour low-temperature fasting culture, the culture is transferred back to fresh natural seawater for feeding culture for 18 hours. During this period, continuous aeration and feeding of single-celled diatoms once every 3 hours are carried out, without water changes or antibiotics; water temperature is measured once every 6 hours.

[0042] 5. Repeat Step 3 ;

[0043] 6. Repeat Step 4 ;

[0044] 7. Repeat Step 2 Examination of the gonads' fullness and color, measurement of the gonadal index, and observation of gonadal histology revealed that 82% of the testes and 87% of the ovaries were in a full state. For example, in the same male and female scallop, the testes were milky white or pale yellow, and the ovaries were yellow. Figure 2 The gonadal indices of the testes and ovaries were 18.32%±0.82% and 23.82%±0.58%, respectively, indicating that the gonadal tissues were in the mature stage. Figure 2 ( ), which can be used as a breeding stock.

[0045] The above embodiments demonstrate that the present invention can efficiently promote the maturation of gonads in *Scallop magnum* broodstock during high-temperature periods. More than 80% of the gonads can develop from the vacuolar stage to maturity within two weeks, far exceeding the 20% in natural sea areas. Gonad-mature broodstock can be directly used for *Scallop magnum* seedling production. Therefore, the present invention has advantages such as high efficiency, short processing time, and strong applicability.

[0046] Example 2

[0047] Time: July 10, 2025 - July 25, 2025; Location: Shantou University Nan'ao Coastal Experimental Station; Natural sea temperature: 28.5±0.4℃.

[0048] On July 10, 2025, 300 healthy, undamaged, and immature male and female *Gnaphalium affine* scallops were selected. Their shells were cleaned of any attached debris and washed thoroughly with natural seawater. They were then raised indoors in natural seawater for one day, followed by a 6-hour period of low-temperature fasting, and then transferred back to fresh natural seawater for 18 hours. During this time, they were continuously aerated and fed single-celled diatoms (food) once every 3 hours. This process was repeated for 14 days until 88% of the scallops reached gonadal maturity. Finally, healthy individuals with mature gonads were selected from this batch for artificial breeding. The specific steps are as follows:

[0049] 1. Selection and rearing of broodstock. Select 300 healthy, undamaged, and immature male and female *Scallop valera* individuals. Clean the shells of any attached organisms and wash them thoroughly with natural seawater. Place them evenly into six 500-L fiberglass culture tanks and rear them in natural seawater indoors using conventional methods for one day. During this period, continuously aerate the tanks, feed them single-celled diatoms once every 3 hours, do not change the water, and do not add antibiotics. Measure the water temperature once every 8 hours.

[0050] 2. Examination of gonadal maturity: After domestication, the fullness and color of the gonads were examined. Three male scallops and three female scallops were randomly selected, and their gonadal index was measured and their gonadal histology was observed. It was found that the testes and ovaries were empty and transparent and colorless. The gonadal indices of the testes and ovaries were 5.80%±0.07% and 7.52%±0.15%, respectively, and the gonadal tissues were in the vacuolar stage.

[0051] 3. Low-temperature fasting culture: After domestication, the breeding shellfish are transferred to a low-temperature recirculating aquaculture system consisting of a chiller and a 500-L fiberglass culture tank. They are kept at a low temperature of 15℃ for 6 hours without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added.

[0052] 4. High-temperature seawater feeding culture; after a 6-hour low-temperature fasting culture, the culture is transferred back to fresh natural seawater for feeding culture for 18 hours. During this period, continuous aeration and feeding of single-celled diatoms once every 3 hours are carried out, without water changes or antibiotics; water temperature is measured once every 6 hours.

[0053] 5. Repeat Step 3 ;

[0054] 6. Repeat Step 4 ;

[0055] 7. Repeat Step 2The fullness and color of the gonads were examined, the gonadal index was measured, and the gonadal histology was observed. It was found that 84% of the testes and 88% of the ovaries were full. The testes were milky white or pale yellow, and the ovaries were yellow. The gonadal indices of the testes and ovaries were 18.54%±0.64% and 24.16%±0.65%, respectively, indicating that the gonadal tissues were all mature and suitable for breeding. Finally, healthy individuals with mature gonads were selected from this batch of scallops as breeding stock (30 males and 120 females). Using conventional methods, 1.4 million healthy seedlings (shell length 0.8mm-1.0mm) were cultivated, and no other problems were found.

[0056] The above embodiments illustrate that the present invention can rapidly mature the gonads of *Scallop luxuriata* broodstock. It can also efficiently mature the gonads of *Scallop luxuriata* broodstock during high-temperature periods, with over 80% of the gonads developing from the vacuolar stage to maturity within two weeks, far exceeding the 20% in natural sea areas. Broodstock with mature gonads can be directly used for *Scallop luxuriata* seedling production. Therefore, the present invention has advantages such as high efficiency, short processing time, and strong applicability.

[0057] Example 3

[0058] Time: August 8, 2025 - August 23, 2025; Location: Shantou University Nan'ao Coastal Experimental Station; Natural sea temperature: 29.5±0.6℃.

[0059] On August 8, 2025, 300 healthy, undamaged, and immature male and female *Gnaphalium affine* scallops were selected. Their shells were cleaned of any attached debris and washed thoroughly with natural seawater. They were then raised indoors in natural seawater for one day, followed by a 6-hour period of low-temperature fasting, and then transferred back to fresh natural seawater for 18 hours. During this time, they were continuously aerated and fed single-celled diatoms (food) once every 3 hours. This process was repeated for 14 days until 85% of the scallops reached gonadal maturity. The specific steps are as follows:

[0060] 1. Selection and rearing of broodstock. Select 300 healthy, undamaged, and immature male and female *Scallop valera* individuals. Clean the shells of any attached organisms and wash them thoroughly with natural seawater. Place them evenly into six 500-L fiberglass culture tanks and rear them in natural seawater indoors using conventional methods for one day. During this period, continuously aerate the tanks, feed them single-celled diatoms once every 3 hours, do not change the water, and do not add antibiotics. Measure the water temperature once every 8 hours.

[0061] 2. Examination of gonadal maturity: After domestication, the fullness and color of the gonads were examined. Three male scallops and three female scallops were randomly selected, and their gonadal index was measured and their gonadal histology was observed. It was found that the testes and ovaries were empty and transparent and colorless. The gonadal indices of the testes and ovaries were 5.02%±0.06% and 7.18%±0.12%, respectively, and the gonadal tissues were in the vacuolar stage.

[0062] 3. Low-temperature fasting culture: After domestication, the breeding shellfish are transferred to a low-temperature recirculating aquaculture system consisting of a chiller and a 500-L fiberglass culture tank. They are kept at a low temperature of 15℃ for 6 hours without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added.

[0063] 4. High-temperature seawater feeding culture; after a 6-hour low-temperature fasting culture, the culture is transferred back to fresh natural seawater for feeding culture for 18 hours. During this period, continuous aeration and feeding of single-celled diatoms once every 3 hours are carried out, without water changes or antibiotics; water temperature is measured once every 6 hours.

[0064] 5. Repeat Step 3 ;

[0065] 6. Repeat Step 4 ;

[0066] 7. Repeat Step 2 The fullness and color of the gonads were examined, the gonadal index was measured, and the gonadal histology was observed. It was found that 84% of the testes and 86% of the ovaries were full. The testes were milky white or pale yellow, and the ovaries were yellow. The gonadal indices of the testes and ovaries were 17.26%±0.58% and 23.44%±0.53%, respectively, indicating that the gonadal tissues were all mature and suitable for breeding. Finally, healthy individuals with mature gonads (35 males and 150 females) were selected from this batch of scallops as breeding stock. Using conventional methods, 2.08 million healthy seedlings (shell length 0.8mm-1.0mm) were cultivated, and no other problems were found.

[0067] The above embodiments demonstrate that the present invention can efficiently promote the maturation of gonads in *Scallop magnum* broodstock during high-temperature periods. More than 80% of the gonads can develop from the vacuolar stage to maturity within two weeks, far exceeding the 20% in natural sea areas. Gonad-mature broodstock can be directly used for *Scallop magnum* seedling production. Therefore, the present invention has advantages such as high efficiency, short processing time, and strong applicability.

[0068] The above-disclosed embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. Therefore, equivalent variations made in accordance with the claims of the present invention are still within the scope of the present invention.

Claims

1. A method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods, characterized in that, include: Shellfish are cultured in alternating hot and cold temperatures to obtain breeding shellfish with mature gonads. The alternating hot and cold temperature aquaculture includes: raising the fish at a low water temperature and then raising it at a high water temperature, and maintaining this cycle for 2 weeks. In each cycle, the culture time at the low water temperature is 6 hours; the culture time at the high water temperature is 18 hours; the low water temperature is 15℃; the high water temperature is 27~31℃; and feeding is prohibited during the culture at the low water temperature in each cycle.

2. The method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 1, characterized in that, The shellfish include scallops.

3. The method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 2, characterized in that, The gonads of the mollusks in question are not fully developed.

4. The method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 2, characterized in that, The shellfish mentioned include broodstock whose gonads are in the vacuolar stage of development.

5. The method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 1, characterized in that, The shellfish mentioned include the noble scallop.

6. The method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 1, characterized in that, include: A. Select the shellfish and raise them in indoor natural seawater under low light conditions. During this period, continuously aerate and feed them, do not change the water or add antibiotics, and monitor the water temperature to obtain domesticated shellfish. B. Transfer the domesticated shellfish into a low-temperature recirculating aquaculture system, and raise them at a low temperature of 15℃ for 6 hours / day without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added. C. Then transfer them back to fresh natural seawater and feed them for 18 hours / day. During this period, continuously aerate and feed them, do not change the water or add antibiotics, and monitor the water temperature. D. Repeat steps B and C for 2 weeks to obtain mature gonadal mollusks.

7. The method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 6, characterized in that, include: A. Select the shellfish and acclimate them in indoor natural seawater with low light for 1 day. During this period, continuously aerate the shellfish, feed them with single-celled diatoms once every 3 hours, do not change the water, do not add antibiotics, and monitor the water temperature once every 8 hours to obtain acclimatized shellfish. B. The domesticated shellfish are transferred into the low-temperature recirculating aquaculture system consisting of a chiller and a 500L fiberglass culture tank. They are kept at a low temperature of 15℃ for 6 hours / day without feeding, during which continuous aeration is provided, the water is not changed, and no antibiotics are added. C. Then transfer them back to fresh natural seawater and feed them for 18 hours / day. During this period, continuously aerate the water, feed them single-celled diatoms once every 3 hours, do not change the water or add antibiotics, and monitor the water temperature once every 6 hours. D. Repeat steps B and C for 2 weeks to obtain mature gonadal mollusks.

8. The application of the method for efficiently promoting the maturation of gonads in warm-water shellfish during high-temperature periods according to claim 1, characterized in that, Used for shellfish seedling cultivation.

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