A confocal detection method for chemical efficacy based on zebrafish model for non-therapeutic purposes and application

Abstract

The application provides a confocal detection method and application of chemical efficacy based on a zebrafish model for non-therapeutic purposes. The application uses KRT4 transgenic red fluorescent strain zebrafish, and carries out UVA damage and image acquisition through a confocal microscope. For sunscreen efficacy detection, the tested chemical is coated on the bottom of a culture dish to form a film, and the sunscreen effect is quantitatively evaluated by comparing the degree of decrease in epidermal fluorescence intensity of the sample group after UVA irradiation with that of the model group. For repair efficacy detection, the zebrafish after damage are incubated with a solution of the tested chemical, and the repair effect is quantitatively evaluated by comparing the degree of recovery of epidermal fluorescence intensity of the sample group after incubation with that of the model group. The application combines the living biological model with the UVA damage and imaging technology of the confocal microscope, realizes direct, quantitative, visual and high-throughput evaluation of the efficacy of chemicals, and overcomes the defects of traditional in vitro methods, such as not close to the biological body, high cost and long cycle of human test.

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