Method for controlling flies by using insecticide

Abstract

The invention discloses a method for controlling flies by using an insecticide, the insecticide is sprayed outdoors, the insecticide comprises pyriproxyfen, deltamethrin, an auxiliary agent and other excipients, the insecticide is prepared into a suspending agent, the weight ratio of the pyriproxyfen to the deltamethrin is 7: 3, the spraying amount is 0.5 L / m < 2 >-5L / m < 2 >, and the outdoors can be a farm, a wasteyard and a fly breeding place. The fly control method can effectively inhibit eclosion of houseflies, and has a good lethal effect on sanitary pests such as fly larvae and mosquitoes.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a method for controlling flies with an insecticide. Background Technology

[0002] Pyriproxyfen, molecular formula: C 20 H 19 NO3, chemical name: 4-phenoxyphenyl(RS)-2-(2-pyridyloxy)propyl ether; pyriproxyphenyl-(RS)-[2-(2-pyridyloxy)propyl] ether. Pyriproxyphenyl is used to control public health pests. It is a phenyl ether insect growth regulator, an inhibitor of chitosan synthesis in the juvenile hormone type. It can also control sweet potato whiteflies and scale insects.

[0003] Deltamethyrin, also known as deltamethrin, is one of the most toxic pyrethroid insecticides. It has both contact and stomach poison effects, with rapid contact action and strong knockdown power. It lacks fumigation and systemic effects, but at high concentrations, it can repel some pests. It has a long residual effect (7-12 days). Formulated as an emulsifiable concentrate or wettable powder, it is a moderately effective insecticide. It has a broad insecticidal spectrum, effective against a variety of pests, including Lepidoptera, Orthoptera, Thysanoptera, Hemiptera, Diptera, and Coleoptera.

[0004] Flies are the fly species most closely related to humans, commonly known as houseflies. They are six to seven millimeters long, densely covered with short hairs, grayish-black in color, with four stripes on their thorax and back, and lack a metallic sheen. Their heads are hemispherical, with licking-sucking mouthparts. They have large compound eyes and three ocelli, and their short, bristle-like antennae have numerous receptors, giving them an extremely sensitive sense of smell. Living in filthy and dirty places, they can carry various bacteria and are one of the "four pests."

[0005] Flies endanger humans by carrying various pathogens and spreading diseases. When flies eat, they vomit and defecate at the same time, expelling the pathogens they ingested along with their digestive fluids, thus contaminating the food they have eaten. If people then eat this food or use the contaminated utensils, they will get sick. The spread of cholera, dysentery, and bacterial food poisoning is directly related to the transmission of diseases by flies.

[0006] Using insecticides to control flies is currently one of the important methods of pest control. Because flies have a short lifespan, high reproductive rate, and wide distribution, and due to increased demands for living environment quality, frequent and excessive use of insecticides has led to flies developing resistance to many chemical insecticides. Physical control methods, such as electronic fly traps, flypaper, and flypaper, are limited in their use and cannot effectively control large numbers of flies. Therefore, fly control remains a challenging and long-term task. Summary of the Invention

[0007] The purpose of this invention is to provide a method for treating flies, which solves the current technical problems of high fly resistance and difficulty in centralized control.

[0008] The technical solution of this invention is:

[0009] A method for controlling flies with an insecticide, characterized in that: monitoring the number of flies outdoors, spraying the insecticide outdoors at a certain spraying amount, wherein the insecticide contains pyriproxyfen, deltamethrin, adjuvants and other excipients, and is made into a suspension, wherein the weight ratio of pyriproxyfen to deltamethrin is 7:3.

[0010] Furthermore, the spraying rate is 0.5 L / m³. 2 -5L / m 2 Optionally, the spraying rate is 1L / m 2 ;

[0011] Furthermore, the outdoor area can be a farm, a garbage dump, or a breeding ground for flies.

[0012] Furthermore, the content of the effective active ingredient in the insecticide is 5%-50%, optionally 5%-30%, and preferably 10%.

[0013] Furthermore, the dosage of the insecticide is 0.25 mL / m 2 -2mL / m 2 The optional dosage is 1 mL / m 2 .

[0014] Furthermore, the fly can be one or more of the following: housefly, green bottle fly, bighead fly, giant-tailed Alyssum, black-tailed flesh fly, stable rot fly, summer toilet fly, and stable stinging fly.

[0015] Furthermore, the additives and excipients are selected from one or more of dispersants, wetting agents, antifreeze agents, preservatives, defoamers, pH adjusters, thickeners, and water.

[0016] The dispersant is selected from one or more of fatty alcohol polyoxyethylene ether phosphate, sodium salt block copolymer of naphthalene sulfonic acid formaldehyde condensate, comb-type polycarboxylate, sodium polycarboxylate, and lignin sulfonate; the wetting agent is selected from one or more of fatty alcohol polyoxyethylene ether, alkyl glycoside, and sodium fatty alcohol polyoxyethylene ether sulfonate; the antifreeze agent is selected from one or more of ethylene glycol, propylene glycol, glycerol, and urea; the preservative is sodium benzoate; the defoamer is a silicone defoamer; the pH adjuster is selected from one or more of glacial acetic acid, citric acid, and triethanolamine; and the thickener is selected from one or more of xanthan gum, magnesium aluminum silicate, and silica.

[0017] Furthermore, the preparation method of the insecticide involves first adding the adjuvants, excipients, active ingredient, and water into a mixing tank and shearing them at high speed to fully disperse them, forming a preliminary dispersion; then, it is transported to a sand mill by a diaphragm pump for pulverization, adjusting the sand mill feed speed to confirm that the particle size meets the requirements; finally, the slurry is transported to a mixing tank, thickeners and defoamers are added to adjust the viscosity of the material, and samples are taken for quality testing to obtain the finished product.

[0018] The insecticide comprises the following components and contents in percentage: pyriproxyfen 3.5%–35%, deltamethrin 1.5%–15%, dispersant 2%–8%, wetting agent 1%–7%, defoamer 0.01%–1%, thickener 0.2%–1%, antifreeze 0%–8%, preservative 0%–1%, pH adjuster 0%–1%, and water as the balance.

[0019] Compared with existing technologies, the method for controlling flies with insecticides described in this invention has the following beneficial effects: it can effectively control the emergence of fly larvae and pupae, has low toxicity, is environmentally safe, and is particularly effective against resistant flies, thus solving the technical problem of the difficulty in controlling flies in a concentrated manner. Detailed Implementation

[0020] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all percentage content ratios, but the present invention is not limited thereto.

[0021] Application Example 1

[0022] Formulation Example 1: 10% Pyriproxyfen·Demeton-methyl Suspension Concentrate

[0023] A 10% pyriproxyfen·deltamethrin suspension was prepared by adding 7% pyriproxyfen technical, 3% deltamethrin technical, 1.5% sodium salt block copolymer of naphthalenesulfonic acid formaldehyde condensate, 1% alkyl glycoside, 1% sodium fatty alcohol polyoxyethylene ether sulfonate, 0.2% silicone defoamer, 0.2% xanthan gum, 2% glycerol, and water to 100%.

[0024] Formulation Example 2: 10% Pyriproxyfen·Demeton-methyl Suspension Concentrate

[0025] A 10% pyriproxyfen·deltamethrin suspension was prepared by adding 7% pyriproxyfen technical, 3% deltamethrin technical, 2% fatty alcohol polyoxyethylene ether phosphate, 3% fatty alcohol polyoxyethylene ether, 0.2% silicone defoamer, 0.2% xanthan gum, 5% ethylene glycol, 0.3% sodium benzoate, and water to 100%.

[0026] Formulation Example 3: 10% Pyriproxyfen·Demeton-methyl Suspension Concentrate

[0027] A 10% pyriproxyfen·deltamethrin suspension was prepared by adding 7% pyriproxyfen technical, 3% deltamethrin technical, 4% lignin sulfonate, 2% sodium fatty alcohol polyoxyethylene ether sulfonate, 0.3% silicone defoamer, 0.2% magnesium aluminum silicate, 4% glycerol, 0.5% sodium benzoate, and water to 100%.

[0028] Formulation Example 4: 10% Pyriproxyfen·Demeton-methyl Suspension Concentrate

[0029] A 10% pyriproxyfen·deltamethrin suspension was prepared by adding 7% pyriproxyfen technical, 3% deltamethrin technical, 4% sodium polycarboxylate, 4% alkyl glycoside, 0.2% silicone defoamer, 0.8% sodium benzoate, 2% urea, 0.2% triethanolamine, 1.2% silica, and water to 100%.

[0030] Formulation Example 5: 50% Pyriproxyfen·Demeton-methyl Suspension Concentrate

[0031] A 50% pyriproxyfen·deltamethrin suspension was prepared by adding 35% pyriproxyfen technical, 15% deltamethrin technical, 3% lignin sulfonate, 2% sodium salt block copolymer of naphthalenesulfonic acid formaldehyde condensate, 3% alkyl glycoside, 3% fatty alcohol polyoxyethylene ether, 0.4% silicone defoamer, 0.1% magnesium aluminum silicate, 4% ethylene glycol, 1% sodium benzoate, and water to 100%.

[0032] Formulation Example 6: 30% Pyriproxyfen·Demeton-methyl Suspension Concentrate

[0033] A 30% pyriproxyfen·deltamethrin suspension was prepared by adding 21% pyriproxyfen technical, 9% deltamethrin technical, 4% comb-type polycarboxylate, 3% sodium fatty alcohol polyoxyethylene ether sulfonate, 0.2% silicone defoamer, 1% magnesium aluminum silicate, 4% tripropylene glycol, 1.2% sodium benzoate, and water to 100%.

[0034] Implementation Example 2:

[0035] Experiment 1: Combined Indoor Toxicity Determination of Pyriproxyfen, Deltamethrin, and Mixed Pairs in Fly 1. Experimental Objective

[0036] Flies (Musca domestica), belonging to the genus Musca in the subfamily Musinae of the family Muscidae in the order Diptera, are a widely distributed sanitary pest and an important vector for intestinal infectious diseases such as bacillary dysentery and cholera. Currently, they are mainly controlled by pesticides. The purpose of this experiment is to determine the toxicity of the combination of pyriproxyfen and deltamethrin to fly larvae and its optimal ratio, providing a basis for enterprise formulation processing and registration applications.

[0037] 2 Experimental Design

[0038] 2.1 Reagents

[0039] 2.1.1 Test reagents

[0040] 97% pyriproxyfen technical grade, provided by the applicant;

[0041] 98% deltamethrin technical grade, provided by the applicant.

[0042] 2.2 Test Treatment

[0043] High-concentration stock solutions were prepared by dissolving pyriproxyfen and deltamethrin technical grade in a small amount of acetone. Five groups of pyriproxyfen and deltamethrin mixtures were then prepared at ratios of 3:7, 5:5, 7:3, 8:2, and 9:1. Based on the preliminary experiments, the pyriproxyfen, deltamethrin stock solutions, and their mixtures were diluted with acetone in a uniform gradient to obtain five series of drug solutions of different mass concentrations.

[0044] 3. Handling methods

[0045] 3.1 Processing time and number of times

[0046] Treat once when the larvae are in the second instar.

[0047] 3.2 Method of administration

[0048] The method of mixing pesticides with artificial feed shall be carried out in accordance with the relevant methods of "Agricultural Industry Standard of the People's Republic of China, Guidelines for Indoor Bioassay Tests of Pesticides, Insecticides, Part 10: Method of Mixing Pesticides with Artificial Feed (NY / T1154.10—2008)".

[0049] 4. Test methods

[0050] 1 ml of the drug solution was transferred using a pipette and added to a mortar containing 100 g of larval feed to prepare a series of toxic feeds at concentrations (64, 32, 16, 8, and 4 mg / kg). Twenty test larvae (2nd instar larvae) were inoculated into 600 mL cans containing 20 g of the toxic feed and then placed in a light incubator for further rearing. Each treatment was repeated four times, with an acetone treatment (without the drug) serving as a control.

[0051] 5. Data Survey and Statistical Analysis

[0052] 5.1 Survey Methodology

[0053] The total number of insects treated was investigated.

[0054] 5.2 Survey Time and Frequency

[0055] Investigate the mortality of test insects 96 hours after treatment and record the total number of insects and the number of dead insects.

[0056] 5.3 Calculation Method

[0057] Based on the survey data, the corrected mortality rate for each treatment was calculated, and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.

[0058] The corrected mortality rate is calculated according to formula (1), and the unit is percentage (%). The calculation result is rounded to two decimal places.

[0059] P=[(Pt-Po)÷(1-Po)]×100…………………………………………(1)

[0060] In the formula: P is the corrected mortality rate; Pt is the treatment mortality rate; Po is the blank control mortality rate.

[0061] The actual toxicity index, theoretical toxicity index, and co-toxicity coefficient are calculated according to equations (2), (3), and (4), respectively.

[0062] Actual toxicity index = LC50 (standard reagent) / LC50 (test reagent) × 100……………(2) Theoretical toxicity index = Actual toxicity index of standard reagent × Percentage of standard reagent in mixture + Actual toxicity index of test reagent × Percentage of test reagent in mixture…………(3) Co-toxicity coefficient (CTC) = Actual toxicity index of mixture / Theoretical toxicity index of mixture × 100…(4)

[0063] Note: The standard reagent referred to in this experiment can be any single agent in the mixture.

[0064] 6 Experimental Results

[0065] Table 1. Combined toxicity of pyriproxyfen and deltamethrin on flies.

[0066]

[0067] Table 1 shows that pyriproxyfen and deltamethrin have different chemical structures and mechanisms of action. Their combined use helps overcome or delay the development of fly resistance and improves control efficacy. Experimental results indicate that different ratios of pyriproxyfen and deltamethrin show synergistic effects on flies (larvae), with the 7:3 ratio exhibiting the strongest activity and co-toxicity coefficients exceeding 158, demonstrating a significant synergistic effect.

[0068] Application Example 3: Indoor application of pesticides to fly larvae as described in Example 1

[0069] 1. Experimental Objective

[0070] Evaluation of the indoor efficacy of 10% pyriproxyfen·deltamethrin suspension against fly larvae.

[0071] 2. Test Basis

[0072] Refer to GB / T 31718-2015 "Technical Specification for Integrated Vector Control", NY / T1964.1-2010 "Indoor Testing of Sanitary Insecticides for Pesticide Registration - Insect Breeding Methods Part 1: Flies", and CEMPS-SOP-716.03 "Bioassay of Fly Larvae (Indoor)".

[0073] 3 Experimental Locations

[0074] This experiment was conducted in an indoor laboratory.

[0075] 4 test subjects

[0076] Experimental subject: flies (Musca domestica), which were raised in the laboratory for a long period of time.

[0077] 5. Experimental Design and Arrangement

[0078] 5.1 Reagent Preparation

[0079] This experiment used 1L / m 2 Spraying rate, formulation dosage 1 mL / m 2 Follow the recommended dosage (1 mL preparation / m 2 Dilute the mother liquor 100 times with water to prepare 5 different concentrations of drug solution (0.02 mL preparation / m 2 0.01 mL formulation / m 2 0.005 mL formulation / m 2 0.0025 mL formulation / m 2 0.00125 mL formulation / m 2 )

[0080] 5.2 Pesticide Spraying

[0081] Following the rearing methods for housefly larvae in NY / 1964.1-2010, wheat bran and milk powder were mixed evenly. A culture medium for housefly larvae was prepared by adding 50 mL of water to every 30 g of the mixture. For each treatment, 30 second-instar housefly larvae in a consistent physiological state were selected, and five different concentrations of pesticide solution were sprayed at 8 ml each. A control group with the same dosage of water was used. The experiment was repeated three times, and the number of pupae and the number of pupae emerging were recorded. The mortality rate was also calculated.

[0082] 5.3 Results Statistics

[0083] Based on the number of adult flies that died in the experiment, the LC was calculated using SPSS 13.0 statistical software. 50 LC 90 The regression equation is y = a + bx. If the mortality rate of the blank control is <5%, no correction is needed; if the mortality rate of the blank control is between 5% and 20%, correction should be performed; if the mortality rate of the blank control is >20%, the trial is invalid.

[0084]

[0085] 6 Experimental Results

[0086] Table 2. Results and evaluation of the efficacy test on housefly larvae in Example 1.

[0087]

[0088]

[0089] Table 2 shows that, in Example 1, five different concentrations of 10% pyriproxyfen·deltamethrin suspension were prepared and used to treat housefly larvae. The results showed that the suspension had a good killing effect on houseflies and a good inhibitory effect on pupa emergence, demonstrating significant control efficacy. Application Example 4: Field efficacy experiment of Example 2 on fly larvae.

[0090] 1. Experimental Location

[0091] Accumulated chicken manure.

[0092] 2. Selection of test subjects, crops and varieties

[0093] Experimental subject: Housefly larvae (Musca domestica), sourced from outdoors.

[0094] 3 Experimental Methods

[0095] Refer to GB / T 27784-2011 "On-site Efficacy Determination and Evaluation of Sanitary Insecticides - General Principles", GB / T 31718-2015 "Technical Specifications for Integrated Vector Control", GB / T 23796-2009 "Determination Methods for Vector Density - Flies" and CEMPS-SOP-715.03 "Efficacy Tests for On-site Control of Fly Larvae (Outdoor)".

[0096] (1) Select breeding farms or garbage dumps, investigate the breeding grounds of flies, and check whether there are fly larvae in the breeding grounds.

[0097] (2) Select 1m 2 The breeding grounds of fly larvae were used as the experimental area.

[0098] (3) Select three test sites within the selected test area to check the number (density) of fly larvae. The inspection method refers to the visual inspection method for larvae in GB / T 23796-2009, specifically points 3 and 4.

[0099] (4) Follow the dosage of 1 mL preparation / m 2 Spraying rate: 0.5 L / m 2 -5 L / m 2 Use a constant volume sprayer to evenly spray 1L of the solution onto the surface to be treated, and cover it with a mesh after spraying.

[0100] (5) Starting on the 8th day after applying the pesticide, check the number of adult flies in the netting. After checking each day, drive away and remove all adult flies from the netting.

[0101] (6) 1m of untreated nearby 2 The area served as a control test area. The experiment was repeated three times.

[0102] 4. Results Statistics

[0103] The relative density decrease rate is calculated according to formulas (1) and (2).

[0104]

[0105] R d = (1-RPI) × 100% (2)

[0106] Where: RPI - Relevant density index; Ta - Average density value of the test area after treatment; Tb - Average density value of the test area before treatment; Ca - Average density value of the control area after treatment; Cb - Average density value of the control area before treatment; Rd - Relative density decrease rate.

[0107] 5 Experimental Results

[0108] Table 3 Example 2 Field Experiment for Controlling Fly Larvae

[0109]

[0110] Table 4. Test data on the efficacy of pesticides in controlling housefly larvae in Example 2 (field trial).

[0111]

[0112] The results of the field experiment show that, according to 1 mL of preparation / m 2 Treatment of housefly larvae has a good control effect.

[0113] Application Example 5: Indoor Efficacy Test for Fly Control (Example 3)

[0114] 1. Experimental Location

[0115] This experiment was conducted in an indoor laboratory.

[0116] 2. Selection of test subjects, crops and varieties

[0117] Experimental subject: Housefly (Musca domestica), 2-3 larvae.

[0118] 3. Experimental Basis

[0119] "Indoor testing of sanitary insecticides for pesticide registration - Insect rearing methods - Part 1: Houseflies" (NY / T1964.1-2010) and "Indoor efficacy testing and evaluation of sanitary insecticides for pesticide registration - Part 5: Mosquito larval control agents" (NY / T1151.5-2014).

[0120] 4. Drug Preparation

[0121] According to the spraying rate of 0.5L / m 2 -5 L / m 2 This experiment uses 1L / m 2 The recommended dosage is 1 mL / m 2 .

[0122] 5 Experimental Steps

[0123] Add fly larvae feed to a white enamel dish, and then add 200±20 2nd-3rd instar housefly larvae to the dish. Following the recommended spraying rate of 1 L / m², evenly spray approximately 60 mL of the test agent onto the feed. Spray an equal amount of water onto the larvae feed. Serve as a crop control group. After 24 hours, observe and record the number of dead larvae daily. Collect fly pupae into a clean container, and observe and record the number of pupae that hatch and the number of adult flies. After 72 hours, remove the pupae and surviving larvae, and raise them normally. Observe and record the number of dead larvae, pupae, and adult flies daily. The test is repeated three times.

[0124] 6 Calculation Methods

[0125] Summarize the data and calculate using the following formula:

[0126]

[0127] 7 Experimental Results

[0128] Table 5. Results and evaluation of the efficacy test on housefly larvae in Example 3.

[0129] test reagents pupation rate (%) Feathering inhibition rate (%) Corrected feathering inhibition rate (%) evaluate Experimental Group 1 99.48 100 100 Significant effect Experimental Group 2 98.93 100 100 Significant effect Experimental Group 3 98.94 100 100 Significant effect control group 99.54 89.04 10.96 —

[0130] As shown in Table 5, the experimental group in Example 3 had a significant inhibitory effect on the emergence of housefly pupae, with an inhibition rate of 100%.

[0131] Application Example 6: Field Efficacy Test for Fly Control (Example 4)

[0132] 1. Experimental Location

[0133] This experiment was conducted at a site where feces were piled up in an experimental base.

[0134] 2. Selection of test subjects, crops and varieties

[0135] Experimental subjects: housefly (Musca domestica) and golden housefly (Chrysomya megacephala), larvae and pupae.

[0136] 3. Drug Preparation

[0137] This experiment uses 1L / m 2 The recommended dosage is 1 mL / m 2 Take 3 mL of the preparation and prepare 3 L for later use.

[0138] 4 Experimental Steps

[0139] According to the experimental design, three experimental groups and one control group were set up. Five 10cm × 10cm sampling points were selected in each treatment group to check and record the number of fly larvae and pupae, and the pre-experimental fly larval density was calculated. Each experimental group was uniformly sprayed with 1L of diluted pesticide solution, while the control group was uniformly sprayed with an equal amount of water. After spraying, the netting was used to cover the netting. Starting on the 8th day after application, the number of adult flies inside the netting was checked and recorded. After each daily check, all adult flies inside the netting were removed.

[0140] 5. Methods for calculating drug efficacy

[0141] The relative density decrease rate is calculated according to formulas (1) and (2).

[0142]

[0143] R d = (1-RPI) × 100% (2)

[0144] Where: RPI - Relevant density index; Ta - Average density value of the test area after treatment; Tb - Average density value of the test area before treatment; Ca - Average density value of the control area after treatment; Cb - Average density value of the control area before treatment; Rd - Relative density decrease rate.

[0145] 6 Experimental Results

[0146] Table 6 Results of field efficacy test for fly control in Example 4

[0147] Treatment agents Correlation density index (%) Relative density decrease rate (%) evaluate Houseflies 8.23 91.77 Significant effect Total number of houseflies and golden flies 7.97 92.03 Significant effect

[0148] As can be seen from Table 6, the 10% pyriproxyfen·deltamethrin suspension in Example 4 showed significant field efficacy against housefly and golden maggot larvae and pupae.

Claims

1. A method for controlling flies with an insecticide, characterized in that: The number of flies outdoors is monitored, and an insecticide is sprayed outdoors at a certain spraying amount. The insecticide contains pyriproxyfen, deltamethrin, adjuvants and other excipients, and is made into a suspension. The weight ratio of pyriproxyfen to deltamethrin is 7:

3. The spraying rate is 0.5 L / m³. 2 -5L / m 2 Optionally, the spraying rate is 1 L / m³. 2 ; The outdoor area can be a farm, a garbage dump, or a breeding ground for flies.

2. The method according to claim 1, characterized in that: The effective active ingredient content of insecticides is 5%-50%; Optionally, the content of effective active ingredients is 5%-30%.

3. The method according to claim 2, characterized in that: The effective active ingredient content of the insecticide is 10%.

4. The method according to any one of claims 1 to 3, characterized in that: The dosage of the insecticide is 0.25 mL / m³. 2 -2mL / m 2 The optional dosage is 1 mL / m 2 .

5. The method according to claim 1, characterized in that: The flies mentioned can be one or more of the following: housefly, green bottle fly, bighead fly, giant-tailed Alyssum fly, black-tailed flesh fly, stable rot fly, summer toilet fly, and stable stinging fly.

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