Composition helpful for improving athletic performance and relieving fatigue

Abstract

The invention discloses a composition helpful for improving athletic performance and relieving fatigue, which comprises the following components in parts by weight: 10 to 140 parts of gallocatechin gallate, 10 to 75 parts of sophora flower bud extract, 10 to 80 parts of red spinach powder and 35 to 115 parts of cordyceps militaris extract. The composition is prepared from the following components in parts by weight: 10 to 140 parts of gallocatechin gallate, 10 to 75 parts of sophora flower bud extract, 10 to 80 parts of red spinach powder and 35 to 115 parts of cordyceps militaris extract. Meanwhile, the red amaranth powder, the cordyceps militaris extract, the gallocatechin gallate and the sophora flower bud extract are combined for use, so that the antioxidant activity is further enhanced, and the effect is far better than that of a single component.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of nutraceutical technology, in particular to a composition for improving sports performance and relieving fatigue. BACKGROUND

[0002] Fatigue refers to a temporary decline in function caused by physical or mental overconsumption, which manifests as an inability to maintain the original exercise intensity and leads to reduced sports performance. According to its causes and characteristics, fatigue can be divided into the following types: secondary fatigue: induced by sleep disorders, depression, overwork or drug side effects; physiological fatigue: caused by insufficient rest, physical load or mental stress; chronic fatigue: a state of fatigue formed by long-term accumulation, which can significantly affect an individual's physiological function and exercise capacity, and in severe cases can lead to overwork (extreme physiological failure caused by overwork).

[0003] Patent CN 116869160 A discloses a raw material composition for improving ATP energy metabolism and its preparation method, which contains green tea powder, green coffee powder, red beet powder, red amaranth powder and moringa leaf powder, as well as L-carnitine, water-soluble vitamins, ginger oil microcapsule powder, minerals, black pepper extract and guarana extract, which can inhibit lactic acid accumulation, improve exercise endurance and improve anti-fatigue ability. The above patent lists the formula raw materials, but does not explain "why they are composed in this way" and what kind of technical effects beyond known functions are brought about by such composition; Patent CN201510442567.9 discloses a composition for improving sports performance and relieving fatigue, which is composed of the following raw materials in mass percentage: maca: 13.3-36.4, oat: 13.3-36.4, yohimbine: 6.7-27.3, L-arginine-alpha-ketoglutarate: 26.7-54.5. The composition provided by the invention can activate the autophagy signaling pathway in muscle tissue, reduce serum urea nitrogen content, improve superoxide dismutase activity and strengthen the body's antioxidant stress response, thereby significantly improving sports performance and having a significant effect on relieving fatigue and accelerating fatigue elimination. The above patent only emphasizes a single antioxidant mechanism, does not introduce other mechanisms, does not explain the interaction between different components, and does not find a direct impact of fatigue improvement on sports performance. Yohimbine has potential side effects (including gastrointestinal discomfort, increased heart rate, anxiety and high blood pressure, and in some cases even life-threatening conditions such as heart attack, seizure and acute kidney injury), and dietary supplements are strictly limited in some countries. SUMMARY

[0004] The purpose of this invention is to provide a composition that helps improve athletic performance and relieve fatigue. By simultaneously using red amaranth powder, cordyceps militaris extract, gallic catechin gallate, and sophora japonica extract, the antioxidant activity is further enhanced, and the effect is far greater than that of a single ingredient, thereby solving the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a composition that helps improve athletic performance and relieve fatigue, comprising gallic catechin gallate, sophora japonica extract, red amaranth powder, and cordyceps militaris extract, wherein the weight parts of gallic catechin gallate, sophora japonica extract, red amaranth powder, and cordyceps militaris extract are 10-140 parts, 10-75 parts, 10-80 parts, and 35-115 parts, respectively.

[0006] Preferably, the weight parts of gallic catechin gallate, Sophora japonica extract, red amaranth powder, and Cordyceps militaris extract are 55-140 parts, 10-40 parts, 20-80 parts, and 35-80 parts, respectively.

[0007] Preferably, the composition contains 68-136 parts by weight of gallic catechin gallate, 19-38 parts by weight of sophora japonica extract, red amaranth powder, and cordyceps militaris extract, respectively.

[0008] Preferably, the content of gallocatechin gallate is above 90%, the content of quercetin in the Sophora japonica extract is above 30%, the content of red amaranth powder is 9% nitrate and 13% potassium ions, and the content of cordycepin in the Cordyceps militaris extract is above 0.2%.

[0009] Preferably, the composition can be applied to relieve exercise fatigue, increase the body's antioxidant activity, and improve athletic performance.

[0010] Preferably, the composition can be prepared into various dosage forms, including but not limited to powders, tablets, direct-drinking powders, hard capsules, soft capsules, gummies, oral liquids, etc.

[0011] Preferably, the powder product comprises, by weight: 68 parts EGCG, 38 parts Sophora japonica extract, 44 parts red amaranth powder, 58 parts Cordyceps militaris extract, 200 parts dried tangerine peel powder, 60 parts bitterness masking agent, 8 parts steviol glycosides, 0.4 parts vitamin B2, 0.4 parts vitamin B1, and 11.2 parts inulin.

[0012] Preferably, the composition can be prepared into different dosage forms for use alone, and the composition can be added in the form of a mixing packet to sports supplements containing vitamin B1 and vitamin B2, peptides, creatine, branched-chain amino acids, protein powder, etc.

[0013] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention is the first to discover that the combined use of red amaranth powder and cordyceps militaris extract has a synergistic antioxidant effect. Furthermore, the combined use of red amaranth powder, cordyceps militaris extract, gallic catechin gallate, and sophora japonica extract further enhances the antioxidant activity, with effects far greater than those of a single ingredient. 2. The invention integrates three core pathways—NO-mediated blood flow improvement, antioxidant / anti-inflammatory defense, and energy regulation—through multi-pathway synergy, rather than simply adding up the raw materials; 3. This invention is the first to discover that the combined use of red amaranth powder, cordyceps militaris extract, gallic catechin gallate, and sophora japonica extract mainly works by synergistically resisting oxidation, scavenging ROS, increasing SOD enzyme activity, increasing ATP energy reserves, relieving fatigue, and thus increasing exercise distance. 4. All raw materials used in this invention are new food ingredients or derived from natural food ingredients, with no side effects and good safety. Attached Figure Description

[0014] Figure 1 The DPPH radical scavenging rate of the sample of this invention; Figure 2 The results of the DPPH free radical scavenging activity assay of the sample of this invention; Figure 3 The effect of the sample of this invention on oxidative stress in zebrafish; Figure 4 The effect of the sample of this invention on SOD activity in zebrafish; Figure 5 The effect of the invention's sample on the movement distance of fatigued zebrafish; Figure 6 The effect of the sample on the ATP content in fatigued zebrafish. Detailed Implementation

[0015] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0016] Please see Figures 1-6This invention provides a technical solution: a composition that helps improve athletic performance and alleviate fatigue, comprising gallic acid gallate, Sophora japonica extract, red amaranth powder, and Cordyceps militaris extract. The oxidative stress mechanism of exercise fatigue: During strenuous exercise, the oxygen flux to active skeletal muscles increases, leading to the production and accumulation of excessive reactive oxygen species (ROS). The accumulation of ROS puts the body in a state of oxidative stress, causing exercise fatigue through the following pathways: directly damaging mitochondrial membrane structure and respiratory chain proteins, inhibiting ATP synthesis; causing intracellular Ca ion regulation imbalance, reducing muscle contractility and endurance; stimulating the NF-κB pathway, triggering an inflammatory response, and delaying post-exercise recovery. These physiological changes ultimately contribute to the occurrence of exercise fatigue and a decline in athletic performance. Nitric oxide (NO) is a signaling molecule involved in multiple physiological processes, including... Skeletal muscle contraction, endothelial function, mitochondrial biosynthesis and respiration, muscle repair, and antioxidant defense are all crucial functions during exercise (especially aerobic exercise). Therefore, improving nitric oxide production to enhance endurance performance has become a current research hotspot. Gallic catechin gallate (EGCG) has been reported to possess antioxidant properties, inhibit obesity, protect the liver, and have anti-cancer effects. Some studies have reported that it can increase serum creatine kinase and liver antioxidant enzymes, and reduce blood whey levels, thereby prolonging exhaustion swimming time. Sophora japonica flower extract (with quercetin as its main active ingredient) has strong antioxidant activity, anti-inflammatory, and immunomodulatory effects. Reports indicate that quercetin can enhance the body's antioxidant capacity, regulate immunity (such as reducing TNF-α and increasing IL-10 levels), reduce muscle damage, promote glucose metabolism and muscle function, and improve anti-fatigue effects. Cordyceps militaris extract, whose main active components are cordycepin and adenosine, can activate the AMPK and PPAR-γ pathways, increase mitochondrial synthesis and function, promote ATP production and inhibit excessive ATP consumption, and maintain energy homeostasis. Some studies also suggest that it can increase NO synthesis, improve oxygen uptake, thereby alleviating exercise fatigue and improving athletic performance. Red amaranth is a high-quality source of nitrates in the diet and can act as a precursor to exogenous NO, rapidly increasing NO levels in the body, thereby dilating blood vessels, improving muscle blood flow and oxygen perfusion, reducing exercise oxygen consumption, and improving exercise efficiency. Furthermore, it can accelerate the excretion of metabolic wastes such as lactic acid, relieve muscle soreness, and promote recovery. It is also rich in K+, which helps maintain electrolyte balance during exercise and prevent muscle cramps. The weight parts of gallic catechin gallate, sophora japonica extract, red amaranth powder and cordyceps militaris extract are 10-140 parts, 10-75 parts, 10-80 parts and 35-115 parts, respectively.

[0017] The weight parts of gallic catechin gallate, Sophora japonica extract, red amaranth powder and Cordyceps militaris extract are 55-140 parts, 10-40 parts, 20-80 parts, and 35-80 parts, respectively. The composition contains 68-136 parts by weight of gallic catechin gallate, 19-38 parts by weight of Sophora japonica extract, red amaranth powder, and Cordyceps militaris extract, respectively. The content of gallic catechin gallate is over 90%, the content of sophora japonica extract and quercetin is over 30%, the content of red amaranth powder is 9% nitrate and 13% potassium ions, and the content of cordycepin is over 0.2% in cordycepin extract. The composition can be applied to relieve exercise fatigue, increase the body's antioxidant activity, and improve athletic performance; The composition can be prepared into various dosage forms, including but not limited to powders, tablets, direct drinking powders, hard capsules, soft capsules, gummies, oral liquids, etc. The powder products, by weight, include: 68 parts EGCG, 38 parts Sophora japonica extract, 44 parts red amaranth powder, 58 parts Cordyceps militaris extract, 200 parts dried tangerine peel powder, 60 parts bitterness masking agent, 8 parts steviol glycosides, 0.4 parts vitamin B2, 0.4 parts vitamin B1, and 11.2 parts inulin. The composition can be prepared into different dosage forms for use alone, or it can be added in the form of a mix pack to sports supplements containing vitamin B1 and vitamin B2, peptides, creatine, branched-chain amino acids, protein powder, etc., as an additional active ingredient.

[0018] Working principle: When using this invention: Experimental Example 1: Screening of In Vitro DPPH Free Radical Scavenging Activity Experimental materials and instruments: Samples and reagents: EGCG, Sophora japonica flower bud extract, red amaranth powder, Cordyceps militaris extract, DPPH (2,2-biphenyl-1-picrylhydrazine), anhydrous ethanol, distilled water, dimethyl sulfoxide (DMSO). Instruments: Ultrasonic cleaner, UV-VIS ultraviolet spectrophotometer, analytical balance, vortex oscillator.

[0019] Experimental methods Preparation of main reagents: Prepare DPPH solution, store in the dark, and use immediately after preparation; Determination of the DPPH radical scavenging activity of the sample: DPPH radicals have a strong absorption peak at 517 nm in the visible light region. The sample reacts with DPPH radicals, causing the characteristic purple color of DPPH radicals to disappear, turning it colorless or pale yellow. The absorbance after the reaction was measured using a UV-Vis spectrophotometer.

[0020] The amounts of each solution added to the experimental system are shown in the table below: Group DPPH solution (ml) Anhydrous ethanol (ml) Sample (ml) Blank group (Ab) 3 1 0 Sample to be tested group (As) 3 0 1 Add the test solution according to Table 1, and measure the absorbance value of each group at 517 nm. Calculate the DPPH free radical scavenging rate of each group of samples according to the following formula.

[0021] P (%) = ((Ab-As) / Ab)×100% Ab: Absorbance value of the blank group; As: Absorbance value of the sample group to be tested.

[0022] A linear equation (R²≥0.99) was established between the amount of the sample solution to be tested and the clearance rate (R²≥0.99) to calculate the amount of sample required to clear 50% of DPPH free radicals (IC50).

[0023] Sample information is shown in the table below: Group Formulation composition Weight fraction Comparative example 1 Red amaranth powder 211 Sample 1 Comparative example 2 Cordyceps militaris extract 196 Sample 2 Comparative example 3 Red amaranth powder extract: Cordyceps militaris extract 105：98 Sample 3 Comparative example 4 EGCG: red amaranth powder: Cordyceps militaris extract 45：89：77 Sample 4 Comparative example 5 Sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 38：89：77 Sample 5 Example 1 EGCG: sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 11：75：11：96 Sample 6 Example 2 EGCG: sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 136：19：22：38 Sample 7 Example 3 EGCG: sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 68：19：44：77 Sample 8 Example 4 EGCG: sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 57：9：78：67 Sample 9 Example 5 EGCG: sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 34：28：22：115 Sample 10 Example 6 EGCG: sophora flower bud extract: red amaranth powder: Cordyceps militaris extract 68：38：44：58 Sample 11 Experimental results Depend on Figure 1 It can be seen that red amaranth powder and Cordyceps militaris extract alone have a certain DPPH free radical scavenging rate in vitro. However, when red amaranth powder and Cordyceps militaris extract are used in combination, the DPPH free radical scavenging of sample 3 is significantly increased, approximately twice that of sample 1 (red amaranth powder) and 4.35 times that of sample 2 (Cordyceps militaris extract). This indicates that the combined use of Cordyceps militaris extract and red amaranth powder has a strong antioxidant synergistic effect.

[0024] Experimental Example 2. Functional Activity Experiment of Samples on Zebrafish Oxidative Stress Model Experimental methods: Experimental animals: wild-type AB strain zebrafish, incubated in a 28°C incubator; Experimental model: Except for the normal group, all other groups established a zebrafish oxidative stress model by administering menadione in water. Experimental groups: There were a total of 5 experimental groups, including 1 normal group, 1 model control group, and 3 sample groups, with 30 zebrafish in each experimental group.

[0025] Detection methods: Cell ROX Green Reagent staining was used to analyze the fluorescence intensity of the yolk sac; SOD activity in zebrafish was tested using an SOD activity assay kit. Data processing: Statistical results are expressed as mean ± SD. Statistical analysis was performed using SPSS IBM software. P < 0.05 was considered statistically significant.

[0026] Experimental results: Effects of the samples on oxidative stress in zebrafish: such as Figure 3As shown, compared with the normal group, the fluorescence intensity of the yolk sac in the model control group was significantly increased (P<0.001), indicating that the oxidative stress model was established. Compared with the model control group, the fluorescence intensity of samples 6, 7, and 11 were all significantly decreased (P<0.001), showing a dose-response relationship (data not shown). This demonstrates that all samples can exert good antioxidant activity in vivo and effectively reduce damage caused by oxidative stress. The effect of samples on SOD activity: such as Figure 4 As shown, compared with the normal group, the SOD activity in the model control group was significantly decreased (P<0.001). Compared with the model group, all three samples significantly increased the SOD activity in zebrafish (P<0.01), indicating that samples 6, 7, and 11 can effectively improve the antioxidant activity in zebrafish, thereby alleviating oxidative stress damage.

[0027] Experiment Example 2: Functional Activity Experiment of Samples on Zebrafish Fatigue Model Experimental methods: Experimental animals: wild-type AB strain zebrafish, incubated in a 28°C incubator; Experimental model: Except for the normal group, all other groups established a zebrafish fatigue model by administering anhydrous sodium sulfite in water. Experimental groups: There were a total of 5 experimental groups, including 1 normal group, 1 model control group, and 3 sample groups, with 30 zebrafish in each experimental group.

[0028] Detection methods: The total distance traveled by zebrafish over 1 hour was measured using a behavior analyzer; the ATP content of each group of zebrafish was measured using an ATP assay kit. Data processing: Statistical results are expressed as mean ± SD. Statistical analysis was performed using SPSS IBM software. P < 0.05 was considered statistically significant.

[0029] Experimental results: Effect of the sample on the movement distance of fatigued zebrafish Figure 5 It was found that, compared with the normal group, the total movement distance of zebrafish in the model control group was significantly reduced, by approximately 45% (P<0.001). Compared with the model control group, the total movement distance of samples 6, 7, and 11 was significantly increased, with sample 11 showing the largest increase, 1.67 times that of the model control group (P<0.001). Samples 6, 7, and 11 all helped alleviate fatigue and improve exercise endurance performance, with sample 11 showing the best effect. The effect of the sample on ATP content is determined by Figure 6It was found that, compared with the normal group, the ATP content in the zebrafish of the model control group was significantly decreased, with a decrease of approximately 43% (P<0.001). Compared with the model control group, the ATP content in zebrafish of samples 6, 7, and 11 was significantly increased, approximately 1.2 times that of the model control group (P<0.001), indicating that samples 6, 7, and 11 could significantly increase the ATP content in fatigued zebrafish, increase energy reserves, and thus improve athletic performance.

[0030] Example 4. Preparation of a powder product with functions of relieving fatigue and improving athletic performance. A powder formulation (parts by weight) for relieving fatigue and improving athletic performance: EGCG 68 parts, Sophora japonica extract 38 parts, red amaranth powder 44 parts, Cordyceps militaris extract 58 parts, tangerine peel powder 200 parts, bitterness masking agent 60 parts, steviol glycosides 8 parts, vitamin B2 0.4 parts, vitamin B1 0.4 parts, inulin 11.2 parts.

[0031] The preparation method of the powder is as follows: S1 Premix: Weigh out 68 parts of EGCG, 38 parts of Sophora japonica extract, 44 parts of red amaranth powder, 58 parts of Cordyceps militaris extract, and 200 parts of dried tangerine peel powder according to the formula ratio, and mix them to obtain a dry premix. S2 Mixing: Weigh 60 parts of bitterness masking agent, 8 parts of steviol glycosides, 0.4 parts of vitamin B2, 0.4 parts of vitamin B1, and 11.2 parts of inulin, put them into a mixing tank, mix, add the dried premix from step 1, pass through a 40-mesh sieve to prevent clumping, and mix evenly; S3 Filling: Package the material obtained in step 2, 1.2g / packet, to obtain the finished product, once a day.

[0032] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0033] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A composition that helps improve athletic performance and relieve fatigue, comprising gallic acid catechin gallate, sophora japonica extract, red amaranth powder, and cordyceps militaris extract, characterized in that: The weight parts of gallic catechin gallate, Sophora japonica extract, red amaranth powder, and Cordyceps militaris extract are 10-140 parts, 10-75 parts, 10-80 parts, and 35-115 parts, respectively.

2. The composition according to claim 1 that helps improve athletic performance and relieve fatigue, characterized in that: The weight parts of gallic catechin gallate, Sophora japonica extract, red amaranth powder, and Cordyceps militaris extract are 55-140 parts, 10-40 parts, 20-80 parts, and 35-80 parts, respectively.

3. The composition according to claim 2 that helps improve athletic performance and relieve fatigue, characterized in that: The composition contains 68-136 parts by weight of gallic catechin gallate, 19-38 parts by weight of sophora japonica extract, red amaranth powder, and cordyceps militaris extract, respectively.

4. The composition according to claim 1 that helps improve athletic performance and relieve fatigue, characterized in that: The content of gallic catechin gallate is above 90%, the content of quercetin in the Sophora japonica extract is above 30%, the content of red amaranth powder is 9% nitrate and 13% potassium ions, and the content of cordycepin in the Cordyceps militaris extract is above 0.2%.

5. A composition according to claims 1-4 that helps improve athletic performance and relieve fatigue, characterized in that: The composition can be applied to relieve exercise fatigue, increase the body's antioxidant activity, and improve athletic performance.

6. A composition according to claims 1-4 that helps improve athletic performance and relieve fatigue, characterized in that: The composition can be prepared into various dosage forms, including but not limited to powders, tablets, direct-drinking powders, hard capsules, soft capsules, gummies, oral liquids, etc.

7. The composition according to claim 6 that helps improve athletic performance and relieve fatigue, characterized in that: The powder product comprises, by weight: 68 parts EGCG, 38 parts Sophora japonica extract, 44 parts red amaranth powder, 58 parts Cordyceps militaris extract, 200 parts dried tangerine peel powder, 60 parts bitterness masking agent, 8 parts steviol glycosides, 0.4 parts vitamin B2, 0.4 parts vitamin B1, and 11.2 parts inulin.

8. A composition according to claims 1-4 that helps improve athletic performance and relieve fatigue, characterized in that: The composition can be prepared into different dosage forms for use alone, and the composition can be added in the form of a mixing packet to sports supplements containing vitamin B1 and vitamin B2, peptides, creatine, branched-chain amino acids, protein powder, etc.

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