Dendrobium officinale and cistanche compound ointment with nourishing effect and preparation method of dendrobium officinale and cistanche compound ointment

Abstract

The invention discloses dendrobium officinale and cistanche compound ointment with a nourishing effect and a preparation method thereof, and relates to the technical field of traditional Chinese medicine nourishing preparations. The compound ointment comprises dendrobium officinale, desert cistanche, American ginseng, astragalus membranaceus, fructus lycii, red dates, black sesame, raspberry, folium cortex eucommiae, lucid ganoderma, sharkskin, malt paste and the like, part of the raw materials can be subjected to exclusive pretreatment, and compound natural antioxidant components with specific purity can also be added and subjected to inclusion treatment. The preparation method sequentially comprises the steps of raw material preparation, raw material pretreatment and cleaning, slicing and soaking, decoction treatment, filtration and sterilization, filling operation, packaging treatment and the like, by optimizing the raw material treatment mode and the preparation process, the dissolution efficiency of effective components of the raw materials is improved, it is guaranteed that the ointment is free of fishy smell, the system is stable, the finished product is appropriate in moisture content, and the good nourishing effect is achieved; the industrial stable production can be realized.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine tonic preparations, specifically relating to a compound ointment of Dendrobium officinale and Cistanche deserticola with tonic effects and its preparation method. Background Technology

[0002] In the field of traditional Chinese medicine tonics, ointments have long been favored by the market due to their convenient administration and easy absorption of active ingredients. Dendrobium officinale possesses both yin-nourishing and heat-clearing properties, as well as stomach-tonifying and fluid-generating effects, while Cistanche deserticola tonifies kidney yang and replenishes essence and blood. The synergistic effect of these two ingredients has been recognized by both traditional medical theory and modern research, and compound tonics based on these raw materials are gradually becoming a research hotspot. Currently, most commercially available tonic ointments use single or a few tonic herbs, lacking a systematic approach to component combination and having limited application of specialty ingredients, making it difficult to fully leverage the synergistic tonic effects of multiple components.

[0003] Existing processes for processing raw materials and preparing nourishing pastes have several shortcomings. In the raw material pretreatment stage, the failure to remove the lignified core material from *Cistanche deserticola* often affects the taste and the proportion of active ingredients in the paste. The dense cell wall structure of *Dendrobium officinale* makes it difficult to achieve full dissolution of active ingredients using conventional pulverization methods. Some preparations attempt to introduce marine biological raw materials to enhance nutrition, but due to a lack of targeted pretreatment processes, problems such as residual fishy odor and uneven texture easily occur. In terms of preparation processes, traditional boiling methods easily cause the volatilization and loss of heat-sensitive active ingredients, and the insufficient antioxidant stability of the paste limits its shelf life. Furthermore, the limited variety of raw material slicing methods also restricts the dissolution efficiency of active ingredients.

[0004] These technological shortcomings directly result in a difficulty in simultaneously achieving both the nourishing efficacy and quality stability of existing products. This fails to meet consumer demand for highly effective nourishment and also limits the industrial-scale promotion of such compound ointments. The industry urgently needs a technological solution that enables refined pretreatment of raw materials, scientific formulation, and precise process control. This would improve the utilization rate of effective components, flavor quality, and storage stability of ointments, thereby promoting the quality upgrade of traditional Chinese medicine nourishing ointments. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present invention provide a compound ointment of Dendrobium officinale and Cistanche deserticola with nourishing effects and its preparation method, which solves the problems of insufficient systematic compatibility of related nourishing ointments, inadequate pretreatment of special raw materials, easy loss of effective ingredients and insufficient product stability caused by the preparation process in the prior art.

[0006] To address the above problems, the present invention provides the following technical solution: The Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects contains the following components by weight: 55-65 parts Dendrobium officinale, 95-105 parts Cistanche deserticola, 10-15 parts American ginseng, 8-12 parts Astragalus membranaceus, 18-22 parts Lycium barbarum, 45-55 parts jujube, 55-65 parts black sesame, 23-28 parts raspberry, 8-12 parts Eucommia ulmoides leaf, 8-12 parts Ganoderma lucidum, 23-28 parts shark skin, and 95-105 parts malt extract. The finished compound ointment has a moisture content of 8%-12%.

[0007] Furthermore, the desert Cistanche deserticola undergoes low-temperature freezing and core removal treatment, by freezing the desert Cistanche deserticola at -18℃ for 2 hours and then removing the lignified core material; the Dendrobium officinale undergoes a combined cell wall breaking and enzymatic hydrolysis pretreatment, first using an ultra-micro cell wall breaking machine to process it to a particle size of 10-20μm, and then soaking it in a pectinase solution with a pH of 3.5 for 1 hour, the pectinase solution mass concentration being 0.2% and the material-to-liquid ratio being 1:5.

[0008] Furthermore, the American ginseng undergoes vacuum freeze-drying-low temperature pulverization pretreatment, whereby the American ginseng is freeze-dried in a vacuum environment of 0.08 MPa and a temperature of -40℃ for 12 hours, and then pulverized to 80 mesh; the astragalus undergoes honey-processing-ethanol extraction pretreatment, whereby the astragalus is mixed with refined honey at a weight ratio of 10:1 and moistened, then stir-fried at 120℃ for 10 minutes, and then soaked in a 30% ethanol solution for 2 hours. After soaking, the ethanol is recovered under reduced pressure at a vacuum of 0.06-0.08 MPa and a temperature of 50-60℃ until there is no alcohol taste; the black sesame undergoes microwave cooking pretreatment, whereby it is microwaved at a power of 600W for 3 minutes, cooled to room temperature, and then pulverized to 60 mesh.

[0009] Furthermore, the ointment also includes 0.5-2 parts by weight of a compound natural antioxidant component, which is a mixture of grape seed extract, sea buckthorn extract, and amla extract in a weight ratio of 2:1:1. The grape seed extract contains ≥90% proanthocyanidins, the sea buckthorn extract contains ≥15% total flavonoids, and the amla extract contains ≥20% polyphenols. This component is encapsulated using a β-cyclodextrin saturated aqueous solution method, with an encapsulation ratio of antioxidant component to β-cyclodextrin of 1:5 by weight. The encapsulation process involves dissolving β-cyclodextrin in purified water at 60°C to prepare a saturated solution, adding the antioxidant component, stirring at a constant temperature for 1 hour, cooling to room temperature, refrigerating and standing for 12 hours, filtering, and vacuum drying at 45°C for 6 hours to obtain the inclusion complex. This component is added simultaneously with the malt extract in step S4 during preparation.

[0010] Furthermore, the shark skin needs to be pretreated by soaking it in a 2% sodium bicarbonate solution for 1 hour to remove the fishy smell, and then softening it by enzymatic hydrolysis at 35°C for 30 minutes with a 0.3% neutral protease solution with a pH of 7.0. After washing, it is drained and ready for use.

[0011] This invention relates to a compound ointment containing Dendrobium officinale and Cistanche deserticola, which has nourishing effects. Dendrobium officinale and Cistanche deserticola are the core active ingredients, combined with a variety of nourishing ingredients such as American ginseng, Astragalus membranaceus, wolfberry, red dates, black sesame, raspberry, Eucommia ulmoides leaves, and Ganoderma lucidum. Shark skin is also introduced to expand the nutritional supplementation. Malt extract is used to adjust the properties of the ointment system, and the moisture content of the finished product is controlled within a suitable range to ensure the ointment's formability and storage characteristics. Specific pretreatment methods are designed for Cistanche deserticola and Dendrobium officinale to improve the proportion of effective components and dissolution efficiency, and a targeted treatment scheme is designed for shark skin to eliminate flavor defects. Furthermore, the stability of the ointment system is enhanced by adding compound natural antioxidants of specific purity ratios and encapsulation treatment. Overall, this invention achieves systematic component compatibility, refined raw material utilization, and stable product quality, fully leveraging the synergistic nourishing value of multiple components.

[0012] As a general inventive concept, this invention provides a method for preparing a compound ointment of Dendrobium officinale and Cistanche deserticola with nourishing effects as described above, comprising the following steps: S1. Raw material preparation: Weigh each component raw material according to the proportion; S2. Raw material pretreatment and cleaning: First, the shark skin is pretreated to remove fishy smell and soften it, and then all raw materials are graded and ultrasonically cleaned. S3. Slicing and soaking: After cleaning, slice the raw materials that need to be sliced, and then place the sliced ​​raw materials and other unsliced ​​raw materials together in a soaking device for soaking. S4. Boiling process: Transfer the soaked raw materials to a boiling pot for boiling to obtain the boiling liquid; S5. Filtration and sterilization: Filter the boiled liquid and then sterilize the filtered liquid using a pasteurizer to obtain sterilized liquid. S6. Filling operation: Transfer the sterilized liquid to the filling machine for filling; S7. Packaging process: Package the filled product to obtain the finished compound ointment.

[0013] Furthermore, the acceptance criteria for raw materials in step S1 are as follows: impurity content ≤0.3%, total heavy metal content ≤10mg / kg, total organophosphorus pesticide residue ≤0.5mg / kg, polysaccharide content in Dendrobium officinale ≥25%, total echinacoside and verbascoside content in Cistanche deserticola ≥1.5%, polysaccharide content in Ganoderma lucidum ≥1.0%, collagen content in shark skin ≥80%, and no signs of spoilage or deterioration.

[0014] Further, in step S2, the shark skin deodorization and softening pretreatment process involves soaking the shark skin in a 2% sodium bicarbonate solution for 1 hour to remove the odor, and then softening it by enzymatic hydrolysis at 35°C for 30 minutes with a 0.3% neutral protease solution at pH 7.0. In step S2, the graded ultrasonic cleaning involves first cleaning with 20kHz low-frequency ultrasound combined with flowing water for 12 minutes, and then cleaning with 40kHz high-frequency ultrasound combined with purified water for 6 minutes. After cleaning, the shark skin is placed in a draining device at room temperature (20-25°C) and vacuum (0.05MPa) for 5 minutes to remove residual moisture from the surface.

[0015] Further, in step S3, the raw materials to be sliced ​​are desert Cistanche deserticola, Dendrobium officinale, American ginseng, Astragalus membranaceus, Ganoderma lucidum, jujube, Eucommia ulmoides leaves, and raspberry. The thickness of the slices is 2-3 mm, and the slices are cut at a 45° angle. This angled cutting method can increase the contact area between the raw materials and the soaking liquid and decoction liquid, and improve the dissolution efficiency of the effective ingredients. In step S3, soaking involves adding purified water and compound enzyme preparation to the soaking equipment. First, the pH value of the purified water is adjusted to 4.0, and then the compound enzyme preparation is added. The compound enzyme preparation is a mixture of cellulase and pectinase in a weight ratio of 2:1, wherein the cellulase activity is ≥100,000 U / g and the pectinase activity is ≥50,000 U / g. The amount of compound enzyme preparation added is 0.1%-0.3% of the weight of purified water, the material-liquid ratio is 1:8, the soaking temperature is 40-45℃, the soaking time is 2-3 hours, and the mixture is stirred intermittently for 5 minutes every 30 minutes during the soaking process, with a stirring speed of 40 rpm.

[0016] Furthermore, in step S4, the cooking process is carried out in stages under slight negative pressure. First, the cooking pot is evacuated to a vacuum of 0.02 MPa, and the mixture is boiled at 95-100℃ and maintained for 30 minutes. Then, it is cooked at 60-70℃ for 2-2.5 hours. After that, malt extract is added, and cooking continues at 60-70℃ for 30-40 minutes. During the cooking process, a combination of magnetic stirring and mechanical stirring is used simultaneously, with an overall stirring speed of 30-50 rpm. The stirring direction is changed every 20 minutes, i.e., clockwise. After stirring for 20 minutes, switch to counterclockwise stirring for 20 minutes, and repeat until the cooking is complete; in step S5, the filtration is carried out by sequentially using a 150-200 mesh nylon filter cloth for coarse filtration, a 500 mesh polyester filter cloth for fine filtration, and a 0.22μm polytetrafluoroethylene filter membrane for final filtration; the sterilization process conditions in step S5 are a temperature of 75-80℃, a holding time of 15-20 minutes, and after sterilization, a gradient cooling method is used, first reducing the temperature to 45℃ and holding it for 10 minutes, and then reducing it to below 25℃ at a cooling rate of 5℃ / min.

[0017] The present invention discloses a method for preparing a compound paste containing Dendrobium officinale and Cistanche deserticola with nourishing effects. First, it establishes multi-dimensional acceptance standards from the source of raw materials to ensure the uniformity of raw material quality. Then, it designs differentiated pretreatment schemes based on the physicochemical properties of different raw materials, eliminating flavor defects of specialty raw materials such as shark skin and improving the accessibility of effective components from core raw materials such as Dendrobium officinale and Cistanche deserticola. By optimizing the contact morphology of raw materials and the enzymatic soaking system, it enhances the dissolution efficiency of effective components. It reduces the loss of heat-sensitive components by relying on a micro-negative pressure compound stirring cooking method. Combined with graded filtration and gradient cooling processes, it ensures the uniformity and stability of the paste system. Simultaneously, it precisely controls the feeding points of antioxidant components, achieving synergistic compatibility between the entire preparation process and the paste formulation. Ultimately, it achieves a unity of nourishing effects, flavor quality, and storage stability, and possesses the technical feasibility for stable industrial production.

[0018] Compared with the prior art, the advantages of the present invention are as follows: (1) This invention uses Dendrobium officinale and Cistanche deserticola as the core functional components, combined with a variety of nourishing ingredients and introduces shark skin to expand the nutritional supplementation dimension. The effects of each component are complementary, which can give full play to the synergistic nourishing value of nourishing yin and kidney, replenishing qi and nourishing blood, etc., and meet the diversified nourishing needs. (2) This invention breaks through the structural limitations of the raw materials by removing the core from the desert Cistanche deserticola, performing a combined pretreatment of Dendrobium officinale with cell wall breaking and enzymatic hydrolysis, and using a specific oblique cutting method for the raw materials, thereby increasing the contact area of ​​the effective ingredients and significantly improving the dissolution efficiency and absorbability of the core functional ingredients. (3) The present invention has designed a special deodorizing and softening treatment scheme for shark skin, which completely eliminates its inherent fishy smell defect. At the same time, it uses malt paste to adjust the properties of the paste system and control the moisture content of the finished product, so that the paste has a uniform texture, suitable taste, good formability and is not prone to problems such as layering and crystallization. (4) By adding a complex natural antioxidant component that has been encapsulated, the present invention can effectively delay the oxidation and deterioration of the active ingredients in the ointment. Combined with gradient cooling and graded filtration processes, the ointment system is kept stable, the product storage period is extended, and the active ingredients can be maintained for a long time. (5) This invention establishes a multi-dimensional raw material acceptance standard, controls the purity and active ingredient content of each raw material from the source, and combines it with a standardized preparation process to keep the quality of each batch of products stable and avoid quality differences caused by fluctuations in raw materials or processes. (6) The parameters of each step of the preparation process of the present invention are clear and smoothly connected. The micro-negative pressure cooking and compound stirring processes are not only suitable for the characteristics of multi-component raw materials, but also compatible with existing formulation equipment, which can realize large-scale stable production and meet the market's demand for batch supply. Attached Figure Description

[0019] Figure 1This is a flowchart of the present invention, which describes a compound ointment containing Dendrobium officinale and Cistanche deserticola with nourishing effects and its preparation method. Detailed Implementation

[0020] To facilitate understanding of the present invention, the invention will be described more fully and in detail below with reference to the accompanying drawings and preferred embodiments, but the scope of protection of the present invention is not limited to the following specific embodiments.

[0021] Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of the invention.

[0022] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods. Example 1

[0023] refer to Figure 1The present invention comprises a compound ointment of Dendrobium officinale and Cistanche deserticola with nourishing effects, which, by weight, contains 60 parts of Dendrobium officinale, 100 parts of Cistanche deserticola, 12 parts of American ginseng, 10 parts of Astragalus membranaceus, 20 parts of wolfberry, 50 parts of red dates, 60 parts of black sesame, 25 parts of raspberry, 10 parts of Eucommia ulmoides leaf, 10 parts of Ganoderma lucidum, 25 parts of shark skin, 100 parts of malt extract, and 1 part by weight of compound natural antioxidant components. The compound natural antioxidant component is composed of grape seed extract, sea buckthorn extract, and amla extract in a weight ratio of 2:1:1. The grape seed extract contains ≥90% proanthocyanidins, the sea buckthorn extract contains ≥15% total flavonoids, and the amla extract contains ≥20% polyphenols. This component is encapsulated using a β-cyclodextrin saturated aqueous solution method, with an encapsulation ratio of antioxidant component to β-cyclodextrin of 1:5 by weight. The desert cistanche undergoes low-temperature freezing to remove the core; the desert cistanche is frozen at -18℃ for 2 hours before the lignified core is removed. The dendrobium officinale undergoes a combined cell wall breaking and enzymatic hydrolysis pretreatment; it is first processed using an ultra-micro cell wall breaking machine to a particle size of 15μm, then soaked in a pectinase solution with a pH of 3.5 for 1 hour (pectinase solution mass concentration 0.2%, material-to-liquid ratio 1:5). The American ginseng undergoes vacuum freeze-drying and low-temperature pulverization pretreatment. The astragalus root is freeze-dried for 12 hours at a vacuum of 0.08 MPa and a temperature of -40℃, and then pulverized to 80 mesh. The astragalus root undergoes a honey-processing-ethanol extraction pretreatment: astragalus root and refined honey are mixed at a weight ratio of 10:1, moistened, stir-fried at 120℃ for 10 minutes, and then soaked in a 30% ethanol solution for 2 hours. After soaking, the ethanol is recovered under reduced pressure at a vacuum of 0.07 MPa and a temperature of 55℃ until no alcohol odor remains. The black sesame seeds undergo microwave cooking pretreatment: microwaved at 600W for 3 minutes, cooled to room temperature, and then pulverized to 60 mesh. The shark skin undergoes a deodorizing and softening pretreatment: the shark skin is soaked in a 2% sodium bicarbonate solution for 1 hour to remove the odor, and then softened by enzymatic hydrolysis at 35℃ for 30 minutes with a 0.3% pH 7.0 neutral protease solution. After washing, it is drained and ready for use. The finished compound ointment has a moisture content of 10%.

[0024] The preparation method of the Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects in this embodiment includes the following steps: S1. Raw material preparation: Weigh each component raw material according to the proportion; S2. Raw material pretreatment and cleaning: First, the shark skin is pretreated to remove the fishy smell and soften it. The shark skin is soaked in a 2% sodium bicarbonate solution for 1 hour to remove the fishy smell. Then, it is softened by enzymatic hydrolysis with a 0.3% neutral protease solution with a pH of 7.0 at 35°C for 30 minutes. Then, all raw materials are subjected to graded ultrasonic cleaning. First, 20kHz low-frequency ultrasound is used to clean with flowing water for 12 minutes. Then, 40kHz high-frequency ultrasound is used to clean with purified water for 6 minutes. After cleaning, the raw materials are placed in a draining device at room temperature of 22°C and a vacuum of 0.05MPa for 5 minutes to remove residual moisture on the surface. S3. Slicing and Soaking: After cleaning, slice the raw materials to be sliced. The raw materials to be sliced ​​are desert Cistanche deserticola, Dendrobium officinale, American ginseng, Astragalus membranaceus, Ganoderma lucidum, red dates, Eucommia ulmoides leaves, and raspberries. The slice thickness is 2.5mm, and the slices are cut at a 45° angle. Then, place the sliced ​​raw materials and other unsliced ​​raw materials together in a soaking device for soaking. Add purified water and compound enzyme preparation to the soaking device. First, adjust the pH value of the purified water to 4.0, and then add the compound enzyme preparation. The compound enzyme preparation is a mixture of cellulase and pectinase in a weight ratio of 2:1, wherein the cellulase activity is ≥100,000 U / g and the pectinase activity is ≥50,000 U / g. The amount of compound enzyme preparation added is 0.2% of the weight of purified water, the material-to-liquid ratio is 1:8, the soaking temperature is 42℃, the soaking time is 2.5h, and the mixture is stirred intermittently for 5min every 30min during the soaking process at a stirring speed of 40 rpm. S4. Cooking process: After soaking, the raw materials are transferred to a cooking pot for segmented cooking under slight negative pressure. First, the cooking pot is evacuated to a vacuum degree of 0.02MPa, and the mixture is boiled at 98℃ and maintained for 30 minutes. Then, it is cooked at 65℃ for 2.2 hours. After that, malt extract is added and the complex natural antioxidant components encapsulated by β-cyclodextrin are added simultaneously. The mixture is then cooked at 65℃ for 35 minutes. During the cooking process, a combination of magnetic stirring and mechanical stirring is used. The overall stirring speed is 40 rpm, and the stirring direction is changed every 20 minutes. That is, the stirring is clockwise for 20 minutes and then counterclockwise for 20 minutes. This cycle is repeated until the cooking is completed, and the cooked liquid is obtained. S5. Filtration and sterilization: The boiled liquid is filtered sequentially using a 180-mesh nylon filter cloth for coarse filtration, a 500-mesh polyester filter cloth for fine filtration, and a 0.22μm polytetrafluoroethylene filter membrane for final filtration. Then, a pasteurizer is used to sterilize the filtered liquid. The sterilization process conditions are: temperature 78℃, holding time 18min. After sterilization, a gradient cooling method is used: first, the temperature is lowered to 45℃ and held for 10min, and then the temperature is lowered to below 25℃ at a cooling rate of 5℃ / min to obtain the sterilized liquid. S6. Filling operation: Transfer the sterilized liquid to the filling machine for filling; S7. Packaging process: Package the filled product to obtain the finished compound ointment. Example 2

[0025] The difference between this embodiment and Example 1 is that no compound natural antioxidant component was added, while the remaining components and preparation methods are completely consistent with Example 1.

[0026] Comparative Example 1 The difference between this comparative example and Example 1 is that the desert Cistanche deserticola was not subjected to low-temperature freezing and core removal treatment, while the remaining components and preparation methods are completely consistent with Example 1.

[0027] Comparative Example 2 The difference between this comparative example and Example 1 is that Dendrobium officinale was only processed to a particle size of 15μm using an ultra-micro cell wall disruptor, and was not soaked in pectinase solution. The remaining components and preparation methods are completely consistent with those of Example 1.

[0028] Comparative Example 3 The difference between this comparative example and Example 1 is that the shark skin was not pretreated to remove fishy odor and soften, but was directly subjected to graded ultrasonic cleaning before being put into subsequent processes. The other components and preparation methods are completely consistent with those of Example 1.

[0029] Comparative Example 4 The difference between this comparative example and Example 1 is that the raw material to be sliced ​​was cut in a conventional 2.5mm thickness cross-section without 45° oblique cutting. The other components and preparation methods are completely consistent with Example 1.

[0030] Comparative Example 5 The difference between this comparative example and Example 1 is that the cooking process was carried out under normal pressure with unidirectional mechanical stirring. No micro-negative pressure was used, and magnetic stirring was not turned on. The stirring direction was not changed, and only a clockwise unidirectional stirring of 40 rpm was maintained. The other components and preparation methods were completely consistent with Example 1.

[0031] Comparative Example 6 The difference between this comparative example and Example 1 is that the composite natural antioxidant component was not subjected to the inclusion treatment by the β-cyclodextrin saturated aqueous solution method, but was directly added to the malt extract in step S4. The remaining components and preparation methods are completely consistent with those of Example 1.

[0032] The ointments from Examples 1-2 and Comparative Examples 1-6 were tested using the following methods: Dissolution rate of core functional components of traditional Chinese medicine: High performance liquid chromatography was used to determine the dissolution amount of Dendrobium officinale polysaccharide, Cistanche deserticola echinacoside and verbascoside, and Ganoderma lucidum polysaccharide. The proportion of each component's dissolution amount to the total content of the corresponding component in the raw material was calculated and the weighted average value was taken. Dissolution rate of shark skin collagen: The content of collagen in the finished product was determined by the Kjeldahl method, and the dissolution rate was calculated by combining the initial collagen content in the raw materials. Flavor rating: An evaluation panel consisting of 10 professional sensory evaluators was formed. In accordance with the "Sensory Evaluation Standards for Health Foods", blind evaluations were conducted from dimensions such as fishy smell, smoothness of mouthfeel, and impurity sensation, and the average score was taken. The specific reasons for deductions were also noted. Retention rate of active ingredients after 6 months of accelerated storage: The sample was stored in an accelerated storage environment of 37°C and 75% relative humidity for 6 months. The heat-sensitive components were tracked by ultraviolet spectrophotometry and other core components were determined by high performance liquid chromatography. The ratio of the content of the components after storage to the initial content of the finished product after decoction was calculated. Paste uniformity rating: Professional testing personnel assess whether the paste has layering or crystallization by static observation method, combined with the microscopic distribution under a microscope, and quantitatively score according to the texture uniformity standard and mark the defect type. The relative value of comprehensive nourishing efficacy was calculated by weighting the dissolution rate of the core functional components of traditional Chinese medicine (weight 60%), the dissolution rate of shark skin collagen (weight 20%), and the in vitro DPPH free radical scavenging rate (weight 20%) as indicators, and the relative value was converted based on the calculation results of Example 1.

[0033] Table 1: Experimental Results of Examples 1-2 and Comparative Examples 1-6

[0034] In summary, referring to Table 1, Example 1 showed the best performance in terms of dissolution rate of core active ingredients of traditional Chinese medicine, dissolution rate of shark skin collagen, flavor, retention rate of effective ingredients during accelerated storage, uniformity of paste, and comprehensive nourishing effects.

[0035] Example 2, due to the absence of the compound natural antioxidant component, only showed a significant decrease in storage retention rate, while the other indicators were similar to those of Example 1.

[0036] Comparative Example 1: Because the desert Cistanche deserticola was not subjected to low-temperature freezing and core removal treatment, the remaining lignified core material not only reduced the dissolution efficiency of the core active ingredients of traditional Chinese medicine, but also gave the paste a rough texture and a slight grainy feel, thus lowering the flavor and uniformity scores and overall nourishing efficacy. Comparative Example 2: Only Dendrobium officinale underwent cell wall breaking without enzymatic hydrolysis, failing to fully destroy its dense cell wall structure, resulting in a significant decrease in the dissolution rate of the core active ingredients of traditional Chinese medicine, directly affecting the overall nourishing efficacy. Comparative Example 3: The shark skin was not pre-treated to remove fishy odor and soften, which inhibited the dissolution of collagen, left a noticeable fishy smell, and caused local collagen clumping, resulting in a decrease in flavor and uniformity scores, while also weakening the overall efficacy. Example 4 uses conventional transverse cutting instead of 45° oblique cutting, resulting in insufficient contact area between the raw materials and solvents. This reduces the dissolution rate of the core functional components of traditional Chinese medicine, thus decreasing the overall tonic effect. Comparative Example 5 uses unidirectional stirring and simmering under normal pressure, which causes some heat-sensitive components to volatilize and be lost. Furthermore, uneven mixing of materials leads to localized burnt and bitter tastes and layering and crystallization of the paste. This results in a decrease in the dissolution rate, storage retention rate, flavor, and uniformity scores of the core functional components of traditional Chinese medicine, significantly reducing the overall efficacy. In Comparative Example 6, the compound natural antioxidant components were not treated with β-cyclodextrin inclusion complexation. The antioxidant active ingredients are easily oxidized and deactivated during storage, leading to a significant decrease in the retention rate of effective components after accelerated storage, and a slight decrease in the overall tonic effect.

Claims

1. A compound ointment of Dendrobium officinale and Cistanche deserticola with nourishing effects, characterized in that: The compound ointment contains the following components by weight: 55-65 parts Dendrobium officinale, 95-105 parts Cistanche deserticola, 10-15 parts American ginseng, 8-12 parts Astragalus membranaceus, 18-22 parts Lycium barbarum, 45-55 parts jujube, 55-65 parts black sesame, 23-28 parts raspberry, 8-12 parts Eucommia ulmoides leaf, 8-12 parts Ganoderma lucidum, 23-28 parts shark skin, and 95-105 parts malt extract. The finished product of the compound ointment has a moisture content of 8%-12%.

2. The Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 1, characterized in that: The desert Cistanche deserticola underwent low-temperature freezing and core removal treatment. The desert Cistanche deserticola was frozen at -18℃ for 2 hours and then the lignified core material was removed. The Dendrobium officinale underwent a combined cell wall breaking and enzymatic hydrolysis pretreatment. First, it was treated with an ultra-micro cell wall breaking machine to achieve a particle size of 10-20μm, and then soaked in a pectinase solution with a pH of 3.5 for 1 hour. The pectinase solution had a mass concentration of 0.2% and a material-to-liquid ratio of 1:

5.

3. The Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 1, characterized in that: The American ginseng underwent vacuum freeze-drying-low temperature pulverization pretreatment, whereby the American ginseng was freeze-dried for 12 hours in a vacuum environment of 0.08 MPa and -40°C, and then pulverized to 80 mesh. The astragalus underwent honey-processing-ethanol extraction pretreatment, whereby the astragalus and refined honey were mixed at a weight ratio of 10:1 and moistened, then stir-fried at 120°C for 10 minutes, and then soaked in a 30% ethanol solution for 2 hours. After soaking, the ethanol was recovered under reduced pressure at a vacuum of 0.06-0.08 MPa and a temperature of 50-60°C until no alcohol taste remained. The black sesame seeds underwent microwave cooking pretreatment, where they were microwaved at 600W for 3 minutes, cooled to room temperature, and then pulverized to 60 mesh.

4. The Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 1, characterized in that: The ointment also includes 0.5-2 parts by weight of a compound natural antioxidant component, which is a mixture of grape seed extract, sea buckthorn extract, and amla extract in a weight ratio of 2:1:

1. The grape seed extract contains ≥90% proanthocyanidins, the sea buckthorn extract contains ≥15% total flavonoids, and the amla extract contains ≥20% polyphenols. This component is encapsulated using a β-cyclodextrin saturated aqueous solution method. The encapsulation ratio is 1:5 by weight of antioxidant component to β-cyclodextrin. The encapsulation process involves dissolving β-cyclodextrin in purified water at 60°C to prepare a saturated solution, adding the antioxidant component, stirring at a constant temperature for 1 hour, cooling to room temperature, refrigerating and standing for 12 hours, filtering, and vacuum drying at 45°C for 6 hours to obtain the inclusion complex. This component is added simultaneously with the malt extract in step S4 during preparation.

5. The Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 1, characterized in that: The shark skin needs to be pretreated by soaking it in a 2% sodium bicarbonate solution for 1 hour to remove the fishy smell, and then softening it by enzymatic hydrolysis with a 0.3% neutral protease solution at pH 7.0 at 35°C for 30 minutes. After washing, it is drained and ready for use.

6. A method for preparing a compound ointment of Dendrobium officinale and Cistanche deserticola with nourishing effects, characterized in that: Includes the following steps, S1. Raw material preparation: Weigh each component raw material according to the proportion; S2. Raw material pretreatment and cleaning: First, the shark skin is pretreated to remove fishy smell and soften. Then, all raw materials are graded and ultrasonically cleaned. S3. Slicing and soaking: After cleaning, slice the raw materials that need to be sliced, and then place the sliced ​​raw materials and other unsliced ​​raw materials together in a soaking device for soaking. S4. Boiling process: Transfer the soaked raw materials to a boiling pot for boiling to obtain the boiling liquid; S5. Filtration and sterilization: Filter the boiled liquid and then sterilize the filtered liquid using a pasteurizer to obtain sterilized liquid. S6. Filling operation: Transfer the sterilized liquid to the filling machine for filling; S7. Packaging process: Package the filled product to obtain the finished compound ointment.

7. The preparation method of the Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 6, characterized in that: The acceptance criteria for raw materials in step S1 are as follows: impurity content ≤0.3%, total heavy metal content ≤10mg / kg, total organophosphorus pesticide residue ≤0.5mg / kg, polysaccharide content in Dendrobium officinale ≥25%, total echinacoside and verbascoside content in Cistanche deserticola ≥1.5%, polysaccharide content in Ganoderma lucidum ≥1.0%, collagen content in shark skin ≥80%, and no signs of spoilage or deterioration.

8. The preparation method of the Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 6, characterized in that: The shark skin deodorization and softening pretreatment process in step S2 involves soaking the shark skin in a 2% sodium bicarbonate solution for 1 hour to remove the odor, followed by enzymatic hydrolysis with a 0.3% neutral protease solution at pH 7.0 at 35°C for 30 minutes to soften it. The graded ultrasonic cleaning in step S2 involves first cleaning with 20kHz low-frequency ultrasound combined with flowing water for 12 minutes, then cleaning with 40kHz high-frequency ultrasound combined with purified water for 6 minutes. After cleaning, the shark skin is placed in a draining device at room temperature (20-25°C) and vacuum (0.05MPa) for 5 minutes to remove residual moisture from the surface.

9. The preparation method of the Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 6, characterized in that: The raw materials to be sliced ​​in step S3 are desert Cistanche deserticola, Dendrobium officinale, American ginseng, Astragalus membranaceus, Ganoderma lucidum, jujube, Eucommia ulmoides leaves, and raspberry. The thickness of the slices is 2-3 mm, and the slices are cut at a 45° angle. This angled cutting method can increase the contact area between the raw materials and the soaking liquid and decoction liquid, and improve the dissolution efficiency of the effective ingredients. The soaking in step S3 involves adding purified water and compound enzyme preparation to the soaking equipment. First, the pH value of the purified water is adjusted to 4.0, and then the compound enzyme preparation is added. The compound enzyme preparation is a mixture of cellulase and pectinase in a weight ratio of 2:1, wherein the cellulase activity is ≥100,000 U / g and the pectinase activity is ≥50,000 U / g. The amount of compound enzyme preparation added is 0.1%-0.3% of the weight of purified water, the material-liquid ratio is 1:8, the soaking temperature is 40-45℃, the soaking time is 2-3 hours, and the mixture is stirred intermittently for 5 minutes every 30 minutes during the soaking process, with a stirring speed of 40 rpm.

10. The preparation method of the Dendrobium officinale and Cistanche deserticola compound ointment with nourishing effects according to claim 6, characterized in that: In step S4, the cooking process is carried out in stages under slight negative pressure. First, the cooking pot is evacuated to a vacuum of 0.02 MPa, and the mixture is boiled at 95-100℃ and maintained for 30 minutes. Then, it is cooked at 60-70℃ for 2-2.5 hours. After that, malt extract is added, and cooking continues at 60-70℃ for 30-40 minutes. During the cooking process, a combination of magnetic stirring and mechanical stirring is used simultaneously. The overall stirring speed is 30-50 rpm, and the stirring direction is changed every 20 minutes, i.e., clockwise stirring. After 20 minutes, switch to counterclockwise stirring for 20 minutes, and repeat until the cooking is complete; in step S5, the filtration is carried out by sequentially using a 150-200 mesh nylon filter cloth for coarse filtration, a 500 mesh polyester filter cloth for fine filtration, and a 0.22μm polytetrafluoroethylene filter membrane for final filtration; the sterilization process conditions in step S5 are a temperature of 75-80℃, a holding time of 15-20 minutes, and after sterilization, a gradient cooling method is used, first reducing the temperature to 45℃ and holding it for 10 minutes, and then reducing it to below 25℃ at a cooling rate of 5℃ / min.

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