Organic fertilizer for repairing heavy metal contaminated soil and improving quality of white kidney beans and planting method

Abstract

The invention belongs to the field of heavy metal contaminated soil remediation and the field of fermented organic fertilizer. The invention provides a fermented organic fertilizer for repairing heavy metal contaminated soil and improving the quality of white kidney beans, which is prepared by fermenting cow dung, chicken manure, corn straw and exocarpium benincasae as fermentation substrates and bacillus amyloliquefaciens SZ-60 as zymophyte. The invention further provides a planting method for repairing heavy metal contaminated soil and improving the quality of white kidney beans based on the fermented organic fertilizer, and the planting method comprises the steps that seeds are soaked before sowing; carrying out soil preparation on the soil and applying a compound microbial agent; 3-5 days after the compound microbial agent is applied, a soil conditioner is applied to the soil; and then applying a fermented organic fertilizer as a base fertilizer, then sowing, and carrying out topdressing in a pod bearing period. According to the method, the content of the alpha-amylase inhibitor in the white kidney beans can be remarkably increased; the yield per mu of the white kidney beans can reach 446 kg / mu at most, and the white kidney beans have good resistance to heavy metal cadmium.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of remediation of heavy metal contaminated soil and the field of fermented organic fertilizer, relates to the field of white kidney bean planting, and in particular to an organic fertilizer for remediation of heavy metal contaminated soil and improvement of white kidney bean quality and a planting method. BACKGROUND

[0002] White kidney bean is a characteristic crop in high-cold and cool mountainous areas and a traditional crop with both medicinal and edible values, and has high medicinal value. Yunnan Province is the largest main producing area in the world in terms of planting area and commodity scale. The known medicinal functions of white kidney bean include reducing blood sugar, reducing insulin secretion and reducing fat, etc. Up to now, a variety of medicinal ingredients have been isolated from white kidney bean, among which α-amylase inhibitors have attracted the most attention. It has been proved in the literature that α-amylase inhibitors have the effects of weight loss and blood sugar reduction [1] .

[0003] At present, the research on the planting technology of white kidney bean is relatively less, and the related research mainly focuses on the fine adjustment of cultivation management, for example, the research of Gong Jiankang [2] , Yang Haijie [3] , Guo Aimin [4] , Tang Zhongxing [5] , Li Chaoying [6] , Liu Qingju [7] , etc. only summarizes the cultivation scheme and the basic planting matters needing attention, but does not propose a specific scheme for how to solve the important deficiencies in the white kidney bean planting technology (for example, how to improve the planting quality of white kidney bean), and the fertilizers and agents used are conventional. Therefore, according to the observation of the inventors, for many years, the planting technology of white kidney bean has not been able to break through the existing level, and the related research results are rarely focused on the improvement of the content of the core medicinal ingredients of white kidney bean, so that the economic nature of its planting cannot be obviously improved. The inventors have also noticed that researchers have carried out research on the interplanting of white kidney bean with other crops [8-11] , but the results of these researches also do not focus on the improvement of the content of the core medicinal ingredients of white kidney bean.

[0004] One of the main means for people to improve the medicinal economy of white kidney bean planting is to select varieties with high content of medicinal ingredients, for example, Zhang Silu

[12] , etc. found in recent research that the content of α-amylase inhibitors in different varieties of white kidney bean is quite different, among which the content of Baiyun 1 and YWL1 is the highest. Therefore, it is believed that selecting varieties with high content is beneficial to the production of α-amylase inhibitors.

[0005] To the best of the inventors' knowledge, the field has not yet carried out research on how to improve the planting technology of alpha-amylase inhibitor content in white kidney beans. In addition, the field has not yet carried out research on how to repair white kidney bean farmland contaminated by heavy metals to promote white kidney bean production. Therefore, in view of the difficulties faced by the aforementioned white kidney bean planting field and the wide application and huge demand of alpha-amylase inhibitor in white kidney beans in food and medicine, it is important to develop a planting technology that can improve the content of alpha-amylase inhibitor in white kidney beans and make white kidney beans grow well in heavy metal contaminated soil, improve the economic value of farmers planting white kidney beans.

[0006] [1] Wang J Y, Liu Z Z, Hou Z Y, et al. Extraction of alpha-amylase inhibitor from white kidney beans and its product development [J]. Journal of Beijing Agricultural College, 2022, 37(04): 114-117. DOI:10.13473 / j.cnki.issn.1002-3186.2022.0423. [2] Gong J K. White kidney bean high yield cultivation [J]. Yunnan Agriculture, 2001, (04): 8. DOI:CNKI:SUN:YNNY.0.2001-04-007. [3] Yang H J. White kidney bean high yield cultivation technology [J]. Modern Agriculture, 2008, (12): 20-21. DOI:CNKI:SUN:XDHY.0.2008-12-016. [4] Guo A M. White kidney bean cultivation technology [J]. Farmer's Friend, 2013, (07): 11. DOI:CNKI:SUN:NMZF.0.2013-07-010. [5] Tang Z X. White kidney bean cultivation technology [J]. Farmer's Friend, 2012, (09): 3. DOI:CNKI:SUN:NMZF.0.2012-09-002. [6] Li Z Y, Yang H F, Dong X H. Key points of white kidney bean cultivation technology [J]. Vegetables, 2016, (06): 54-55. DOI:CNKI:SUN:SCZZ.0.2016-06-024. [7] Liu Q J. Defects and improvement measures of existing white kidney bean planting technology [J]. Agriculture and Technology, 2017, 37(17): 120-121+146. DOI:CNKI:SUN:NYYS.0.2017-17-052. [8] Yang H W, Yang Y S. High-quality and high-yield cultivation technology of small white kidney beans [J]. Farmer's Friend, 2006, (06): 16. DOI:CNKI:SUN:NMZF.0.2006-06-022. [9] Gao G. High yield cultivation of corn and white kidney beans in intercropping [J]. Agriculture and Technology, 2013, 33(06): 83. DOI: CNKI: SUN:NYYS.0.2013-06-065.

[10] Li Z. High yield and efficient cultivation technology of corn-white kidney bean intercropping [C] / / The First Academic Conference of Suihua City, Heilongjiang Province. [Publisher Unknown], 2009: 62-63.

[11] Wu Y. Efficient and dense planting of white kidney beans intercropped with corn [J]. China Horticulture Digest, 2009, 25(04): 118. DOI: CNKI: SUN:YUWZ.0.2009-04-062.

[12] Zhang S, Tang B, Geng Z, et al. Analysis of the activity and content differences of alpha-amylase inhibitors in different varieties of white kidney beans [J]. Jiangsu Journal of Agricultural Sciences, 2024, 52(07): 179-183. DOI: 10.15889 / j.issn.1002-1302.2024.07.024.

[13] Chi Y, Sun Y, Zhong Y, et al. Extraction and performance of alpha-amylase inhibitors from white kidney beans [J]. Food Science and Technology, 2017, 42(12): 225-230. DOI: 10.13684 / j.cnki.spkj.2017.12.041. SUMMARY

[0007] In view of the defects of the prior art, the purpose of the present application is to provide a fermented organic fertilizer for repairing heavy metal contaminated soil and improving the quality of white kidney beans, and a planting method, to significantly improve the yield, alpha-amylase inhibitor content and resistance to heavy metals of white kidney beans.

[0008] To achieve the above purpose, the present application provides the following technical solutions: A fermented organic fertilizer for repairing heavy metal contaminated soil and improving the quality of white kidney beans, wherein the fermented organic fertilizer is obtained by fermenting cow dung, chicken manure, corn straw and winter melon skin as fermentation substrates, and Bacillus amyloliquefaciens SZ-60 as fermentation bacteria, in a proportion of 10:6~9:2~4:1 by weight. The preservation number of Bacillus amyloliquefaciens SZ-60 is CGMCC No. 8277, and the preservation number of Bacillus subtilis 9A is CCTCC No. M2012493.

[0009] A planting method for repairing heavy metal contaminated soil and improving the quality of white kidney beans, comprising: (1) Soaking the seeds before sowing, and preparing the soil and applying the compound microbial inoculant; (2) 3-5 days after the application of the compound microbial agent, apply soil conditioner to the soil; (3) After the application of the soil conditioner, apply fermented organic fertilizer as base fertilizer, then sow, and apply topdressing during the pod setting period; In step (1), when the seed is soaked, the soaking agent used is a water solution of a wheat straw water extract and a water extract of wax gourd skin mixture, wherein the weight ratio of the wheat straw water extract and the water extract of wax gourd skin mixture is 1:3-6; In step (1), the compound microbial agent used is vermiculite powder adsorbed with Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A; the weight ratio of the Bacillus amyloliquefaciens SZ-60 and the Bacillus subtilis 9A is 1:3, and the weight ratio of the total weight of the Bacillus amyloliquefaciens SZ-60 and the Bacillus subtilis 9A to the vermiculite powder is 1:50; In step (2), the soil conditioner comprises the following components in parts by weight: 5 parts of red mud, 2 parts of biochar, 2 parts of limestone, 1 part of silicon oxide, and 20 parts of humic acid; In step (3), the fermented organic fertilizer is obtained by fermenting cow dung, chicken manure, corn straw, and wax gourd skin as fermentation substrates and Bacillus amyloliquefaciens SZ-60 as fermentation bacteria at a ratio of 10:6-9:2-4:1 by weight; The preservation number of the Bacillus amyloliquefaciens SZ-60 is CGMCC No.8277, and the preservation number of the Bacillus subtilis 9A is CCTCC No.M2012493.

[0010] Preferably, in the soaking agent, the weight ratio of the wheat straw water extract and the water extract of wax gourd skin mixture is 1:4.

[0011] Preferably, in the soaking agent, the weight content of the wheat straw water extract and the water extract of wax gourd skin mixture is 2%.

[0012] Preferably, in step (1), when the seed is soaked with the soaking agent, the soaking time is 2 hours.

[0013] Preferably, in step (3), the fermented organic fertilizer is obtained by fermenting cow dung, chicken manure, corn straw, and wax gourd skin as fermentation substrates at a ratio of 10:8:3:1 by weight.

[0014] Preferably, in step (3), when fermentation is performed, the inoculation amount of the Bacillus amyloliquefaciens SZ-60 is 2%.

[0015] Preferably, in step (3), when fermentation is performed, the fermentation temperature is 35°C, and the fermentation time is 72 hours.

[0016] Preferably, the application amount of the complex microbial inoculant is 10 kg / acre.

[0017] Preferably, the application amount of the soil conditioner is 110-130 kg / acre.

[0018] Preferably, the application amount of the fermented organic fertilizer is 180-200 kg / acre as base fertilizer, and 10 kg / acre as topdressing.

[0019] Advantages of the present application: The present application can significantly increase the content of alpha-amylase inhibitor in white kidney beans, and the content of alpha-amylase inhibitor in the obtained white kidney beans can reach more than 13%. Meanwhile, the present application can make the yield of white kidney beans per acre reach up to 446 kg / acre. In addition, the present application can make white kidney beans have good resistance to heavy metal cadmium in heavy metal contaminated soil. The present application significantly improves the economic value of white kidney bean planting, and has good popularization and application prospect. DETAILED DESCRIPTION

[0020] The present application will be specifically described below through examples. It is necessary to point out here that the following examples are only used to further illustrate the present application, and cannot be understood as limiting the protection scope of the present application. Some non-essential improvements and adjustments made by skilled persons in the art according to the above application content still belong to the protection scope of the present application.

[0021] In the present application, the information of raw materials used is as follows: White kidney bean seeds: Yunbai 1 variety; Bacillus amyloliquefaciens SZ-60: preservation number CGMCC No.8277, purchased from China General Microbiological Culture Collection Center; Bacillus subtilis 9A: preservation number CCTCC No.M2012493, purchased from China Center for Type Culture Collection; Bacillus amyloliquefaciens strain RCD-1: preservation number CGMCC No.7502, purchased from China General Microbiological Culture Collection Center; Vermiculite powder: purchased from Lingshou County Yongqi Mineral Products Co., Ltd.; Wheat straw: collected from local farmland; Winter melon peel: collected from local market; Red mud: collected by the inventor, the composition is shown in Table 1; Table 1

[0022] The heavy metals (Pb, Cd) in the red mud meet the risk screening value of the Soil Environmental Quality Risk Control Standard for Agricultural Soil Pollution (Trial) (GB15618-2018).

[0023] Chitosan, biochar, limestone, silicon oxide: self-owned by the inventor's laboratory; Humic acid: purchased from Wuhan Jiyesheng Chemical Co., Ltd.; Cow dung, chicken manure: collected from local farmers.

[0024] Wheat straw water extract: the wheat straw was crushed, and the wheat straw and water were mixed at a weight ratio of 1:10, and extracted at 80°C for 2 hours. After solid-liquid separation, the filtrate was taken, and the filter residue was mixed with water at a weight ratio of 1:10, and extracted at 80°C for 2 hours. The filtrate was taken and mixed with the filtrate obtained in the first extraction. The filtrate was rotary evaporated to dryness, and the powder was taken.

[0025] Winter melon peel water extract: the winter melon peel was crushed, and the winter melon peel and water were mixed at a weight ratio of 1:8, and extracted at 80°C for 2 hours. After solid-liquid separation, the filtrate was taken, and the filter residue was mixed with water at a weight ratio of 1:8, and extracted at 80°C for 2 hours. The filtrate was taken and mixed with the filtrate obtained in the first extraction. The filtrate was rotary evaporated to dryness, and the powder was taken.

[0026] Example 1

[0027] 1. Test site: the planting site is a white kidney bean planting demonstration garden in a certain place in Yunnan Province, with an altitude of 1620 meters, an average annual temperature of 17.6°C, soil of limestone red soil not contaminated by heavy metals, medium fertility, and the previous crop being wheat.

[0028] 2. Preparation of raw materials and reagents: Preparation of soaking agent: the wheat straw water extract and the winter melon peel water extract were mixed at a weight ratio of 1:4, and then water was added to make the weight content of the wheat straw water extract and the winter melon peel water extract mixture 2%.

[0029] Compound microbial agent: Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A were cultured to the plateau stage respectively, centrifuged, resuspended with PBS, and then centrifuged to obtain the bacterial bodies. The bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A were mixed at a weight ratio of 1:3, and then mixed with vermiculite powder, so that the weight ratio of the total bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to the vermiculite powder was 1:50.

[0030] Preparation of soil conditioner: the following components were mixed by weight: 5 parts of red mud, 2 parts of biochar, 2 parts of limestone, 1 part of silicon oxide, and 20 parts of humic acid.

[0031] Preparation of fermented organic fertilizer: take cow dung, chicken manure, corn straw and winter melon skin as fermentation substrate in the ratio of 10:8:3:1 by weight, inoculate with Bacillus amyloliquefaciens SZ-60 at an inoculation amount of 2%, and ferment at 35°C for 72 hours to obtain the fermented organic fertilizer.

[0032] 3. Planting scheme: 3.1 Before sowing, soak the white kidney bean seeds with the soaking agent for 2 hours at a soaking temperature of 25°C.

[0033] 3.2 Prepare the soil for ploughing to a depth of 20-30 cm, and then evenly apply the compound microbial agent at a rate of 10 kg / acre.

[0034] 3.3 Five days after applying the compound microbial agent, apply the soil conditioner to the soil at a rate of 130 kg / acre.

[0035] 3.4 Two days after applying the soil conditioner, apply the fermented organic fertilizer as basal fertilizer at a rate of 130 kg / acre, and then sow the seeds, sowing 2 seeds per hole at 2000 holes per acre; 3.5 Apply the fermented organic fertilizer as topdressing at the podding stage at a rate of 10 kg / acre.

[0036] Example 2

[0037] 1. Test site: the planting site is a white kidney bean planting demonstration garden in a certain place in Yunnan Province, with an altitude of 1620 meters, an average annual temperature of 17.6°C, soil that is not contaminated by heavy metals, limestone red soil, medium fertility, and the previous crop is wheat.

[0038] 2. Preparation of raw materials and reagents: Preparation of soaking agent: mix wheat straw water extract and winter melon skin water extract in the ratio of 1:3 by weight, and then add water to make the weight content of the mixture of wheat straw water extract and winter melon skin water extract 2%.

[0039] Compound microbial agent: culture Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to the plateau phase respectively, resuspend with PBS after centrifugation, and then centrifuge to obtain the bacterial bodies to prepare dry bacterial bodies; mix the bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A in the ratio of 1:3 by weight, and then mix with vermiculite powder to make the weight ratio of the total bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to vermiculite powder 1:50.

[0040] Preparation of soil conditioner: mix the following components by weight: 5 parts of red mud, 2 parts of biochar, 2 parts of limestone, 1 part of silicon oxide and 20 parts of humic acid.

[0041] Preparation of fermented organic fertilizer: take cow dung, chicken manure, corn straw and winter melon skin as fermentation substrate in the ratio of 10:9:4:1, inoculate with Bacillus amyloliquefaciens SZ-60 at an inoculation amount of 2%, and ferment at 35°C for 72 hours to obtain the fermented organic fertilizer.

[0042] 3. Planting scheme: 3.1 Before sowing, soak the white kidney bean seeds with the soaking agent for 2 hours at a soaking temperature of 25°C.

[0043] 3.2 Prepare the soil by plowing to a depth of 20-30 cm, and then evenly apply the compound microbial agent at a rate of 10 kg / acre.

[0044] 3.3 Three days after applying the compound microbial agent, apply the soil conditioner to the soil at a rate of 130 kg / acre.

[0045] 3.4 Two days after applying the soil conditioner, apply the fermented organic fertilizer as basal fertilizer at a rate of 130 kg / acre, and then sow the seeds, sowing 2 seeds per hole at 2000 holes per acre; 3.5 Apply the fermented organic fertilizer as topdressing at the podding stage at a rate of 10 kg / acre.

[0046] Example 3

[0047] 1. Test site: the planting site is a white kidney bean planting demonstration garden in a certain place in Yunnan Province, with an altitude of 1620 meters, an average annual temperature of 17.6°C, soil that is not contaminated by heavy metals, limestone red soil, medium fertility, and the previous crop is wheat.

[0048] 2. Preparation of raw materials and reagents: Preparation of soaking agent: mix wheat straw water extract and winter melon skin water extract in a weight ratio of 1:6, then add water to make the weight content of the mixture of wheat straw water extract and winter melon skin water extract 2%.

[0049] Compound microbial agent: culture Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to the stationary phase, centrifuge, resuspend in PBS, centrifuge again to obtain the bacterial bodies, and prepare dry bacterial bodies; mix the bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A in a weight ratio of 1:3, and then mix with vermiculite powder to make the weight ratio of the total bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to vermiculite powder 1:50.

[0050] Preparation of soil conditioner: mix the following components by weight: 5 parts of red mud, 2 parts of biochar, 2 parts of limestone, 1 part of silicon oxide and 20 parts of humic acid.

[0051] Preparation of fermented organic fertilizer: take cow dung, chicken manure, corn straw and winter melon skin as fermentation substrate in the ratio of 10:6:2:1 by weight, inoculate with Bacillus amyloliquefaciens SZ-60 at an inoculation amount of 2%, and ferment at 35°C for 72 hours to obtain the fermented organic fertilizer.

[0052] 3. Planting scheme: 3.1 Before sowing, soak the white kidney bean seeds in the soaking agent for 2 hours, and the soaking temperature is 25°C.

[0053] 3.2 Prepare the soil by plowing to a depth of 20-30 cm, and then evenly apply the compound microbial agent at a rate of 10 kg / acre.

[0054] 3.3 Four days after applying the compound microbial agent, apply the soil conditioner to the soil at a rate of 130 kg / acre.

[0055] 3.4 Two days after applying the soil conditioner, apply the fermented organic fertilizer as basal fertilizer at a rate of 130 kg / acre, and then sow the seeds, sowing 2000 holes per acre, and sowing 2 seeds per hole; 3.5 Apply the fermented organic fertilizer as topdressing at the podding stage at a rate of 10 kg / acre.

[0056] Comparative Example 1 This comparative example 1 refers to the cultivation scheme of Li Zhaoying [6] , that is, during seed treatment, first expose the seeds to sunlight for 2 days, and before sowing, mix the seeds with 0.3% of 50% carbendazim wettable powder by seed weight, use 3000 kg / acre of farmyard manure (local human and animal manure) and 30 kg / acre of superphosphate as base fertilizer, apply 8 kg / acre of urea and 15 kg / acre of potassium sulfate at the initial flowering stage. The rest of the scheme is the same as example 1.

[0057] Comparative Example 2 Except that the soaking of seeds with soaking solution is replaced by mixing the seeds with 0.3% of 50% carbendazim wettable powder by seed weight, the rest is the same as example 1.

[0058] Comparative Example 3 Except that Bacillus subtilis 9A is used instead of Bacillus amyloliquefaciens SZ-60 in the preparation of fermented organic fertilizer, the rest is the same as example 1.

[0059] Comparative Example 4 Except that winter melon skin is not added to the fermentation substrate in the preparation of fermented organic fertilizer, the rest is the same as example 1.

[0060] Comparative Example 5 Except that chicken manure is not added to the fermentation substrate in the preparation of fermented organic fertilizer, the rest is the same as example 1.

[0061] Comparative Example 6 Except that Bacillus amyloliquefaciens SZ-60 was not inoculated in the preparation of the fermented organic fertilizer, and natural composting was carried out for 15 days beside the white kidney bean planting demonstration garden, the rest was consistent with Example 1.

[0062] Comparative Example 7 Except that Bacillus amyloliquefaciens SZ-60 was replaced by Bacillus amyloliquefaciens strain RCD-1 in the preparation of the fermented organic fertilizer, the rest was consistent with Example 1.

[0063] Comparative Example 8 Except that no soil conditioner was applied, the rest was consistent with Example 1. Specifically: 1. Test site: The planting site was a white kidney bean planting demonstration garden in a certain place in Yunnan Province, with an altitude of 1620 meters, an average annual temperature of 17.6°C, and a soil of limestone red soil not contaminated by heavy metals, with medium fertility, and the previous crop was wheat.

[0064] 2. Preparation of raw materials and reagents: Preparation of soaking agent: Mix wheat straw water extract and winter melon peel water extract according to a weight ratio of 1:4, then add water to make the weight content of the mixture of wheat straw water extract and winter melon peel water extract 2%.

[0065] Compound microbial agent: Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A were respectively cultured to the plateau phase, centrifuged, resuspended with PBS, and then centrifuged to obtain the bacterial bodies, which were then dried. The bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A were mixed according to a weight ratio of 1:3, and then mixed with vermiculite powder to make the weight ratio of the total bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to vermiculite powder 1:50.

[0066] Preparation of soil conditioner: Mix the following components by weight: 5 parts of red mud, 2 parts of biochar, 2 parts of limestone, 1 part of silicon oxide, and 20 parts of humic acid.

[0067] Preparation of fermented organic fertilizer: Cow dung, chicken manure, corn straw and winter melon peel were used as fermentation substrate in a weight ratio of 10:8:3:1, Bacillus amyloliquefaciens SZ-60 was inoculated at an inoculation amount of 2%, and then fermented at 35°C for 72 hours to obtain the fermented organic fertilizer.

[0068] 3. Planting scheme: 3.1 Before sowing, the white kidney bean seeds were soaked with the soaking agent for 2 hours at a temperature of 25°C.

[0069] 3.2 The soil was plowed to a depth of 20-30 cm, and then the compound microbial agent was uniformly applied at a rate of 10 kg / acre.

[0070] 3.3 5 days after the application of the complex microbial agent, apply the fermented organic fertilizer as base fertilizer at a dosage of 130 kg / mu, and then sow, 2000 holes per mu, 2 seeds per hole; 3.4 Apply the fermented organic fertilizer as topdressing during the podding period at a dosage of 10 kg / mu.

[0071] Experimental Example 1 This experimental example investigates the yield of white kidney beans and the content of α-amylase inhibitors in white kidney beans. When measuring the content of α-amylase inhibitors, the white kidney beans are extracted for α-amylase inhibitors, and the extraction method is based on the scheme of

[13] .

[0072] As shown in Table 2, the present application can significantly increase the yield of white kidney beans and the content of α-amylase inhibitors in white kidney beans.

[0073] Table 2

[0074] This experimental example is an influence factor investigation experiment based on the technical solution of "extract immersion + microbial fertilizer + soil conditioner + fermented organic fertilizer" obtained by the inventors after years of research.

[0075] The scheme adopted by Comparative Example 1 is currently commonly used in the field, and the main purpose is to provide farmers with more standardized planting guidance to ensure that white kidney beans do not reduce yield, but the content of the core medicinal ingredient α-amylase inhibitors in white kidney beans obtained by this method is low. As shown in Comparative Example 2, using the extract developed by the present application for seed soaking has a certain effect on the content of α-amylase inhibitors. In particular, as shown in Comparative Examples 3-7, the configuration of fermented organic fertilizer has a significant effect on the content of α-amylase inhibitors in white kidney beans. Therefore, it can be seen that the extract for seed soaking and the fermented organic fertilizer developed by the present application are the key to increasing the content of α-amylase inhibitors in white kidney beans. At the same time, it can also be seen from Table 1 that the present application can also increase the yield of white kidney beans. According to the inventors' knowledge, using traditional planting technology, the yield of white kidney beans per mu is usually 130-250 kg. In some places, it can reach 300-400 kg / mu, for example, Sanjia Village, Maojie Town, Wuding County, Yunnan Province, achieved a planting effect of nearly 400 kg / mu in 2022.

[0076] In particular, as shown in Comparative Example 8, compared with Example 1, the content of α-amylase inhibitors in the obtained white kidney beans decreased by a lower margin, but the yield of white kidney beans decreased significantly. This indicates that whether to use the soil conditioner of the present application has no obvious effect on the content of α-amylase inhibitors in white kidney beans, but has an important effect on the yield of white kidney beans.

[0077] Experimental Example 2 On the basis of Experimental Example 1, the inventors carried out relevant tests on cadmium contaminated land. The test land was 5 mu of farmland around a lead-zinc mine area in Yunnan Province contaminated by heavy metals, with an altitude of 2500 meters and an average annual temperature of 17.6°C. The pH value of the sampled soil in the obstacle farmland was 5.82, the Cd content was 1.16 mg / kg, which exceeded the risk screening value of the “Soil Environmental Quality Risk Control Standard for Agricultural Land (Trial)” (GB15618-2018), but was lower than the risk control value, belonging to light to moderate pollution obstacle farmland, and the previous crop was barley.

[0078] The test land was divided into 5 areas on average, and each area was tested using the schemes of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 5 and Comparative Example 8 respectively. As shown in Table 3, when the conventional planting scheme shown in Comparative Example 1 was used to plant white kidney beans in soil contaminated by cadmium, the yield of white kidney beans showed a significant decline. However, when the planting scheme of Example 1 was used, the decline in the yield of white kidney beans was smaller, indicating that the planting scheme of the present application made white kidney beans have higher resistance to heavy metal cadmium. In particular, as shown in Comparative Example 8, when the soil conditioner of the present application was not added, the decline in the yield of white kidney beans was very obvious, which indicated that the soil conditioner used in the present application had an important influence on improving the resistance of white kidney beans to cadmium.

[0079] Table 3

[0080] As shown in Table 2, in Comparative Example 1, Comparative Example 2, Comparative Example 5 and Comparative Example 8, the Cd content of white kidney beans exceeded the “Food Safety Standard for Limitation of Contaminants in Food” (GB2762-2022) (Cd≤0.2 mg / kg), while the cadmium content in Example 1 of the present application was much lower than the limit.

Claims

1. A fermented organic fertilizer for repairing heavy metal contaminated soil and improving white kidney bean quality, characterized in that, The fermentation organic fertilizer is obtained by fermentation of cow dung, chicken manure, corn straw and winter melon skin as fermentation substrates and Bacillus amyloliquefaciens SZ-60 as fermentation bacteria in a proportion of 10:6-9:2-4:1 by weight. The preservation number of the Bacillus amyloliquefaciens SZ-60 is CGMCC No.8277, and the preservation number of the Bacillus subtilis 9A is CCTCC No.M2012493.

2. A planting method for repairing heavy metal contaminated soil and improving white kidney bean quality, characterized in that, The planting method comprises: (1) soaking the seeds before sowing, and ploughing the soil and applying the compound microbial agent; (2) applying the soil conditioner to the soil 3-5 days after applying the compound microbial agent; (3) applying the fermentation organic fertilizer as base fertilizer, then sowing, and topdressing during the pod setting period; In step (1), the soaking agent used for soaking the seeds is a water solution of a wheat straw water extract and a winter melon skin water extract mixture, wherein the weight ratio of the wheat straw water extract and the winter melon skin water extract mixture is 1:3-6; In step (1), the compound microbial agent used is vermiculite powder adsorbed with the bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A, wherein the weight ratio of the bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A is 1:3, and the weight ratio of the total weight of the bacterial bodies of Bacillus amyloliquefaciens SZ-60 and Bacillus subtilis 9A to the vermiculite powder is 1:50; In step (2), the soil conditioner comprises the following components in parts by weight: 5 parts of red mud, 2 parts of biochar, 2 parts of limestone, 1 part of silicon oxide and 20 parts of humic acid; In step (3), the fermentation organic fertilizer is obtained by fermentation of cow dung, chicken manure, corn straw and winter melon skin as fermentation substrates and Bacillus amyloliquefaciens SZ-60 as fermentation bacteria in a proportion of 10:6-9:2-4:1 by weight. The preservation number of the Bacillus amyloliquefaciens SZ-60 is CGMCC No.8277, and the preservation number of the Bacillus subtilis 9A is CCTCC No.M2012493.

3. The planting method for repairing heavy metal contaminated soil and improving white kidney bean quality according to claim 2, characterized in that, In the soaking agent, the weight ratio of the wheat straw water extract and the winter melon skin water extract mixture is 1:

4.

4. The planting method for repairing heavy metal contaminated soil and improving white kidney bean quality according to claim 3, characterized in that, In the soaking agent, the weight content of the wheat straw water extract and the winter melon skin water extract mixture is 2%.

5. The method according to claim 4, wherein the method is characterized by, In step (1), when the seeds are soaked with the soaking agent, the soaking time is 2 hours.

6. The method according to claim 2, wherein the method is characterized by, In step (3), the fermentation organic fertilizer is obtained by fermentation of cow dung, chicken manure, corn straw and winter melon skin as fermentation substrates in a proportion of 10:8:3:1 by weight.

7. The method according to claim 2, wherein the method is characterized by, In step (3), when fermentation is performed, the inoculation amount of Bacillus amyloliquefaciens SZ-60 is 2%.

8. The method according to claim 7, wherein the method is characterized by, In step (3), when fermentation is performed, the fermentation temperature is 35℃, and the fermentation time is 72 hours.

9. The method according to claim 2, wherein the method is characterized by, The application amount of the compound microbial agent is 10 kg / acre, and the application amount of the soil conditioner is 110-130 kg / acre.

10. The method for planting white kidney beans in contaminated soil with heavy metals for improving the quality of white kidney beans according to claim 2, characterized in that, When the fermentation organic fertilizer is used as base fertilizer, the application amount is 180-200 kg / acre, and when topdressing is performed, the application amount is 10 kg / acre.

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